RchiOBHm_Chr2g0129161

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0002928
Physical Location & Seq
Forward (+)
44855122 .. 44857462
2341 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ50085

Sequence Viewer

Length: 510 bp
ATGTCAACCAGAACCAGTTTTGTAGACAGCTTTGCTGATACAAAAGATGGCCGCTACAATCCAAAAATGGATCCTGTTATTCCTGTACAAAACTGGAGAAAAGGATCTCAGTGGGTTGTATTGACGAGGAAGCATGCAGAGGTTGTAGTGAAAGACGATACAGTGTTTCCTATGTTTCAGTTGTATTGTAAGAGGAAGTCATTACCTGAGTTTTGGCGGGATCATCCTGTTCCGGCCGATAACTCCAAGGAGCACAATTGCATTCCAGACGAGCATTATGTTCAGACATTACTGGCTGATGTAGATAATATCTACTATGAGACCGAGTATCGAAGAGAATGGTGTAGCAGCAAGGAGAAACCCTCTCCATGCTTTCTTTTTGCCATAAAATTTACCCGGCCTGCTGCTCTCAGGCTTCTCAACACGGTAACATTCAAAATTTTTGTCAATGTTCTAGCTTTTGCTAGGATTTTTTCTAATGTATTTTTGTCAAGGAGTCCCATAGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

169

Amino Acids

19.8

Weight (kDa)

9.06

Isoelectric Point (pI)

38.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 3 - 100 5.9e-23 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 24
AciI CCGC 2 cut(s) 52, 217
AclWI GGATC 4 cut(s) 65, 78, 112, 228
AcoI YGGCCR 2 cut(s) 49, 234
AcsI RAATTY 2 cut(s) 389, 438
AfaI GTAC 1 cut(s) 87
AgsI TTSAA 1 cut(s) 436
AluBI AGCT 2 cut(s) 30, 458
AluI AGCT 2 cut(s) 30, 458
Alw21I GWGCWC 1 cut(s) 255
Alw26I GTCTC 1 cut(s) 314
AlwI GGATC 4 cut(s) 65, 78, 112, 228
AoxI GGCC 3 cut(s) 49, 234, 398
ApeKI GCWGC 2 cut(s) 348, 404
ApoI RAATTY 2 cut(s) 389, 438
ArsI GACNNNNNNTTYG 2 cut(s) 429, 461
AsuC2I CCSGG 1 cut(s) 397
BamHI GGATCC 1 cut(s) 70
Bbv12I GWGCWC 1 cut(s) 255
BbvI GCAGC 2 cut(s) 360, 391
BccI CCATC 1 cut(s) 41
BcnI CCSGG 1 cut(s) 397
BcoDI GTCTC 1 cut(s) 314
BfaI CTAG 2 cut(s) 455, 465
BisI GCNGC 3 cut(s) 52, 349, 405
BlsI GCNGC 3 cut(s) 53, 350, 406
Bme1390I CCNGG 1 cut(s) 397
BmiI GGNNCC 1 cut(s) 72
BmrFI CCNGG 1 cut(s) 397
BplI GAGNNNNNCTC 2 cut(s) 347, 379
BpmI CTGGAG 1 cut(s) 115
BpuMI CCSGG 1 cut(s) 397
BsaI GGTCTC 1 cut(s) 314
BsaJI CCNNGG 1 cut(s) 246
Bse1I ACTGG 3 cut(s) 15, 98, 297
BseDI CCNNGG 1 cut(s) 246
BseGI GGATG 1 cut(s) 223
BseMII CTCAG 3 cut(s) 122, 198, 424
BseNI ACTGG 3 cut(s) 15, 98, 297
BseX3I CGGCCG 1 cut(s) 234
BseXI GCAGC 2 cut(s) 360, 391
Bsh1285I CGRYCG 1 cut(s) 237
BshFI GGCC 3 cut(s) 51, 236, 400
BsiEI CGRYCG 1 cut(s) 237
BsiHKAI GWGCWC 1 cut(s) 255
BsiSI CCGG 2 cut(s) 233, 397
BslFI GGGAC 1 cut(s) 483
BsmAI GTCTC 1 cut(s) 314
BsmFI GGGAC 1 cut(s) 483
BsmI GAATGC 1 cut(s) 261
BsnI GGCC 3 cut(s) 51, 236, 400
Bso31I GGTCTC 1 cut(s) 314
Bsp1286I GDGCHC 1 cut(s) 255
Bsp1407I TGTACA 1 cut(s) 85
Bsp143I GATC 3 cut(s) 70, 104, 220
BspACI CCGC 2 cut(s) 52, 217
BspANI GGCC 3 cut(s) 51, 236, 400
BspCNI CTCAG 3 cut(s) 121, 199, 423
BspLI GGNNCC 1 cut(s) 72
BspPI GGATC 4 cut(s) 65, 78, 112, 228
BspTNI GGTCTC 1 cut(s) 314
BsrGI TGTACA 1 cut(s) 85
BsrI ACTGG 3 cut(s) 15, 98, 297
BssECI CCNNGG 1 cut(s) 246
BssMI GATC 3 cut(s) 70, 104, 220
BssT1I CCWWGG 1 cut(s) 246
Bst4CI ACNGT 2 cut(s) 163, 427
Bst6I CTCTTC 1 cut(s) 328
BstAUI TGTACA 1 cut(s) 85
BstC8I GCNNGC 2 cut(s) 135, 402
BstDEI CTNAG 3 cut(s) 108, 207, 410
BstF5I GGATG 1 cut(s) 223
BstKTI GATC 3 cut(s) 73, 107, 223
BstMAI GTCTC 1 cut(s) 314
BstMBI GATC 3 cut(s) 70, 104, 220
BstMCI CGRYCG 1 cut(s) 237
BstNSI RCATGY 1 cut(s) 137
BstSCI CCNGG 1 cut(s) 395
BstV1I GCAGC 2 cut(s) 360, 391
BstX2I RGATCY 2 cut(s) 70, 104
BstYI RGATCY 2 cut(s) 70, 104
BstZI CGGCCG 1 cut(s) 234
BsuRI GGCC 3 cut(s) 51, 236, 400
BtsCI GGATG 1 cut(s) 223
BtsIMutI CAGTG 2 cut(s) 116, 168
Cac8I GCNNGC 2 cut(s) 135, 402
Csp6I GTAC 1 cut(s) 86
CviAII CATG 2 cut(s) 134, 369
CviJI RGCY 7 cut(s) 30, 51, 236, 296, 400, 415, 458
CviKI_1 RGCY 7 cut(s) 30, 51, 236, 296, 400, 415, 458
CviQI GTAC 1 cut(s) 86
DdeI CTNAG 3 cut(s) 108, 207, 410
DpnI GATC 3 cut(s) 72, 106, 222
DpnII GATC 3 cut(s) 70, 104, 220
EaeI YGGCCR 2 cut(s) 49, 234
EagI CGGCCG 1 cut(s) 234
Eam1104I CTCTTC 1 cut(s) 328
EarI CTCTTC 1 cut(s) 328
EclXI CGGCCG 1 cut(s) 234
Eco130I CCWWGG 1 cut(s) 246
Eco31I GGTCTC 1 cut(s) 314
Eco52I CGGCCG 1 cut(s) 234
EcoT14I CCWWGG 1 cut(s) 246
ErhI CCWWGG 1 cut(s) 246
FaeI CATG 2 cut(s) 137, 372
FaiI YATR 7 cut(s) 135, 173, 279, 318, 370, 386, 503
FaqI GGGAC 1 cut(s) 483
FatI CATG 2 cut(s) 133, 368
FauI CCCGC 1 cut(s) 210
FblI GTMKAC 1 cut(s) 24
Fnu4HI GCNGC 3 cut(s) 52, 349, 405
FokI GGATG 1 cut(s) 210
Fsp4HI GCNGC 3 cut(s) 52, 349, 405
FspBI CTAG 2 cut(s) 455, 465
GluI GCNGC 3 cut(s) 52, 349, 405
GsuI CTGGAG 1 cut(s) 115
HaeIII GGCC 3 cut(s) 51, 236, 400
HapII CCGG 2 cut(s) 233, 397
Hin1II CATG 2 cut(s) 137, 372
HincII GTYRAC 1 cut(s) 6
HindII GTYRAC 1 cut(s) 6
HinfI GANTC 1 cut(s) 496
HpaII CCGG 2 cut(s) 233, 397
Hpy166II GTNNAC 2 cut(s) 6, 25
Hpy188I TCNGA 1 cut(s) 285
Hpy188III TCNNGA 1 cut(s) 266
Hpy8I GTNNAC 2 cut(s) 6, 25
HpyCH4III ACNGT 2 cut(s) 163, 427
HpyCH4V TGCA 2 cut(s) 137, 261
HpyF3I CTNAG 3 cut(s) 108, 207, 410
Hsp92II CATG 2 cut(s) 137, 372
Kzo9I GATC 3 cut(s) 70, 104, 220
LmnI GCTCC 1 cut(s) 250
Lsp1109I GCAGC 2 cut(s) 360, 391
MaeI CTAG 2 cut(s) 455, 465
MaeIII GTNAC 1 cut(s) 427
MalI GATC 3 cut(s) 72, 106, 222
MboI GATC 3 cut(s) 70, 104, 220
MboII GAAGA 1 cut(s) 345
MfeI CAATTG 1 cut(s) 256
MflI RGATCY 2 cut(s) 70, 104
MhlI GDGCHC 1 cut(s) 255
MluCI AATT 3 cut(s) 256, 389, 438
MlyI GAGTC 1 cut(s) 505
MnlI CCTC 4 cut(s) 120, 133, 186, 373
MspI CCGG 2 cut(s) 233, 397
MspR9I CCNGG 1 cut(s) 397
MunI CAATTG 1 cut(s) 256
Mva1269I GAATGC 1 cut(s) 261
NciI CCSGG 1 cut(s) 397
NdeII GATC 3 cut(s) 70, 104, 220
NlaIII CATG 2 cut(s) 137, 372
NlaIV GGNNCC 1 cut(s) 72
NspI RCATGY 1 cut(s) 137
PaeI GCATGC 1 cut(s) 137
PctI GAATGC 1 cut(s) 261
PkrI GCNGC 3 cut(s) 53, 350, 406
PleI GAGTC 1 cut(s) 504
PpsI GAGTC 1 cut(s) 504
PspN4I GGNNCC 1 cut(s) 72
PsuI RGATCY 2 cut(s) 70, 104
RsaI GTAC 1 cut(s) 87
RsaNI GTAC 1 cut(s) 86
SatI GCNGC 3 cut(s) 52, 349, 405
Sau3AI GATC 3 cut(s) 70, 104, 220
SchI GAGTC 1 cut(s) 505
ScrFI CCNGG 1 cut(s) 397
SduI GDGCHC 1 cut(s) 255
SetI ASST 4 cut(s) 32, 144, 208, 460
SphI GCATGC 1 cut(s) 137
Sse9I AATT 3 cut(s) 256, 389, 438
SsiI CCGC 2 cut(s) 52, 217
SspMI CTAG 2 cut(s) 455, 465
StyD4I CCNGG 1 cut(s) 395
StyI CCWWGG 1 cut(s) 246
TaaI ACNGT 2 cut(s) 163, 427
TaqI TCGA 1 cut(s) 331
TaqII GACCGA 1 cut(s) 338
TasI AATT 3 cut(s) 256, 389, 438
TatI WGTACW 1 cut(s) 85
TauI GCSGC 1 cut(s) 54
TscAI CASTG 2 cut(s) 116, 168
TseI GCWGC 2 cut(s) 348, 404
TspRI CASTG 2 cut(s) 116, 168
XapI RAATTY 2 cut(s) 389, 438
XceI RCATGY 1 cut(s) 137
XmiI GTMKAC 1 cut(s) 24
XspI CTAG 2 cut(s) 455, 465
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.