Rh4AG023800

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0002928
Physical Location & Seq
Forward (+)
4670487 .. 4671999
1513 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG023800.1

Sequence Viewer

Length: 303 bp
ATGTCAACCAGAACCAGTTTTGTAGACAGCTTTGCTGATACAAAAGATGGCCACTACAATCCAAAAATGGATCCTGTTATTCCTGTACAAAACTGGAGAAAAGGATCTCAGTGGGTTGTATTGAAGAGGAAGCATGCAGAGGTTGTAGTGAAAGACGATACAGTGTTTCCTATATTTCAGTTGTATTGTAAGAGGAAGTCGTTACCTGAGTTTTGGCGGGATCATCCTGTTCCGGCCGATAACTCCAAGGAGCACAATTGCATTCCACTGCAGACCGTGAACGCCGTGGATGGCACCCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

100

Amino Acids

11.55

Weight (kDa)

8.62

Isoelectric Point (pI)

38.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 3 - 72 5.5e-16 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 293
AccI GTMKAC 1 cut(s) 24
AciI CCGC 1 cut(s) 217
AclWI GGATC 4 cut(s) 65, 78, 112, 228
AcoI YGGCCR 2 cut(s) 49, 234
AfaI GTAC 1 cut(s) 87
AgsI TTSAA 1 cut(s) 124
AluBI AGCT 1 cut(s) 30
AluI AGCT 1 cut(s) 30
Alw21I GWGCWC 1 cut(s) 255
AlwI GGATC 4 cut(s) 65, 78, 112, 228
AoxI GGCC 2 cut(s) 49, 234
BalI TGGCCA 1 cut(s) 51
BamHI GGATCC 1 cut(s) 70
BanI GGYRCC 1 cut(s) 293
Bbv12I GWGCWC 1 cut(s) 255
BccI CCATC 2 cut(s) 41, 284
BceAI ACGGC 1 cut(s) 269
BfmI CTRYAG 1 cut(s) 269
BmiI GGNNCC 2 cut(s) 72, 295
BpmI CTGGAG 1 cut(s) 115
BsaJI CCNNGG 2 cut(s) 246, 285
Bse1I ACTGG 2 cut(s) 15, 98
BseDI CCNNGG 2 cut(s) 246, 285
BseGI GGATG 2 cut(s) 223, 295
BseMII CTCAG 2 cut(s) 122, 198
BseNI ACTGG 2 cut(s) 15, 98
BseX3I CGGCCG 1 cut(s) 234
Bsh1285I CGRYCG 1 cut(s) 237
BshFI GGCC 2 cut(s) 51, 236
BshNI GGYRCC 1 cut(s) 293
BsiEI CGRYCG 1 cut(s) 237
BsiHKAI GWGCWC 1 cut(s) 255
BsiSI CCGG 1 cut(s) 233
BsmI GAATGC 1 cut(s) 261
BsnI GGCC 2 cut(s) 51, 236
Bsp1286I GDGCHC 1 cut(s) 255
Bsp1407I TGTACA 1 cut(s) 85
Bsp143I GATC 3 cut(s) 70, 104, 220
BspACI CCGC 1 cut(s) 217
BspANI GGCC 2 cut(s) 51, 236
BspCNI CTCAG 2 cut(s) 121, 199
BspLI GGNNCC 2 cut(s) 72, 295
BspMAI CTGCAG 1 cut(s) 273
BspPI GGATC 4 cut(s) 65, 78, 112, 228
BspT107I GGYRCC 1 cut(s) 293
BsrGI TGTACA 1 cut(s) 85
BsrI ACTGG 2 cut(s) 15, 98
BssECI CCNNGG 2 cut(s) 246, 285
BssMI GATC 3 cut(s) 70, 104, 220
BssT1I CCWWGG 1 cut(s) 246
Bst4CI ACNGT 2 cut(s) 163, 277
Bst6I CTCTTC 1 cut(s) 119
BstAUI TGTACA 1 cut(s) 85
BstC8I GCNNGC 1 cut(s) 135
BstDEI CTNAG 2 cut(s) 108, 207
BstDSI CCRYGG 1 cut(s) 285
BstF5I GGATG 2 cut(s) 223, 295
BstKTI GATC 3 cut(s) 73, 107, 223
BstMBI GATC 3 cut(s) 70, 104, 220
BstMCI CGRYCG 1 cut(s) 237
BstNSI RCATGY 1 cut(s) 137
BstSFI CTRYAG 1 cut(s) 269
BstX2I RGATCY 2 cut(s) 70, 104
BstYI RGATCY 2 cut(s) 70, 104
BstZI CGGCCG 1 cut(s) 234
BsuRI GGCC 2 cut(s) 51, 236
BtgI CCRYGG 1 cut(s) 285
BtsCI GGATG 2 cut(s) 223, 295
BtsI GCAGTG 1 cut(s) 266
BtsIMutI CAGTG 3 cut(s) 116, 168, 266
Cac8I GCNNGC 1 cut(s) 135
Csp6I GTAC 1 cut(s) 86
CviAII CATG 1 cut(s) 134
CviJI RGCY 3 cut(s) 30, 51, 236
CviKI_1 RGCY 3 cut(s) 30, 51, 236
CviQI GTAC 1 cut(s) 86
DdeI CTNAG 2 cut(s) 108, 207
DpnI GATC 3 cut(s) 72, 106, 222
DpnII GATC 3 cut(s) 70, 104, 220
EaeI YGGCCR 2 cut(s) 49, 234
EagI CGGCCG 1 cut(s) 234
Eam1104I CTCTTC 1 cut(s) 119
EarI CTCTTC 1 cut(s) 119
EclXI CGGCCG 1 cut(s) 234
Eco130I CCWWGG 1 cut(s) 246
Eco52I CGGCCG 1 cut(s) 234
EcoT14I CCWWGG 1 cut(s) 246
ErhI CCWWGG 1 cut(s) 246
FaeI CATG 1 cut(s) 137
FaiI YATR 3 cut(s) 135, 173, 301
FatI CATG 1 cut(s) 133
FauI CCCGC 1 cut(s) 210
FblI GTMKAC 1 cut(s) 24
FokI GGATG 1 cut(s) 210
GsuI CTGGAG 1 cut(s) 115
HaeIII GGCC 2 cut(s) 51, 236
HapII CCGG 1 cut(s) 233
Hin1II CATG 1 cut(s) 137
HincII GTYRAC 1 cut(s) 6
HindII GTYRAC 1 cut(s) 6
HpaII CCGG 1 cut(s) 233
Hpy166II GTNNAC 3 cut(s) 6, 25, 280
Hpy8I GTNNAC 3 cut(s) 6, 25, 280
HpyCH4III ACNGT 2 cut(s) 163, 277
HpyCH4V TGCA 3 cut(s) 137, 261, 271
HpyF3I CTNAG 2 cut(s) 108, 207
Hsp92II CATG 1 cut(s) 137
Kzo9I GATC 3 cut(s) 70, 104, 220
LmnI GCTCC 1 cut(s) 250
LpnPI CCDG 8 cut(s) 22, 28, 79, 87, 96, 219, 240, 246
MaeIII GTNAC 1 cut(s) 201
MalI GATC 3 cut(s) 72, 106, 222
MboI GATC 3 cut(s) 70, 104, 220
MboII GAAGA 1 cut(s) 136
MfeI CAATTG 1 cut(s) 256
MflI RGATCY 2 cut(s) 70, 104
MhlI GDGCHC 1 cut(s) 255
MlsI TGGCCA 1 cut(s) 51
MluCI AATT 1 cut(s) 256
MluNI TGGCCA 1 cut(s) 51
MnlI CCTC 3 cut(s) 120, 133, 186
Mox20I TGGCCA 1 cut(s) 51
MscI TGGCCA 1 cut(s) 51
Msp20I TGGCCA 1 cut(s) 51
MspI CCGG 1 cut(s) 233
MunI CAATTG 1 cut(s) 256
Mva1269I GAATGC 1 cut(s) 261
NdeII GATC 3 cut(s) 70, 104, 220
NlaIII CATG 1 cut(s) 137
NlaIV GGNNCC 2 cut(s) 72, 295
NspI RCATGY 1 cut(s) 137
PaeI GCATGC 1 cut(s) 137
PctI GAATGC 1 cut(s) 261
PspN4I GGNNCC 2 cut(s) 72, 295
PstI CTGCAG 1 cut(s) 273
PsuI RGATCY 2 cut(s) 70, 104
RsaI GTAC 1 cut(s) 87
RsaNI GTAC 1 cut(s) 86
Sau3AI GATC 3 cut(s) 70, 104, 220
SduI GDGCHC 1 cut(s) 255
SetI ASST 3 cut(s) 32, 144, 208
SfcI CTRYAG 1 cut(s) 269
SphI GCATGC 1 cut(s) 137
Sse9I AATT 1 cut(s) 256
SsiI CCGC 1 cut(s) 217
StyI CCWWGG 1 cut(s) 246
TaaI ACNGT 2 cut(s) 163, 277
TasI AATT 1 cut(s) 256
TatI WGTACW 1 cut(s) 85
TscAI CASTG 3 cut(s) 116, 168, 273
TspRI CASTG 3 cut(s) 116, 168, 273
XceI RCATGY 1 cut(s) 137
XmiI GTMKAC 1 cut(s) 24
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.