AT5G14550

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0002928
Physical Location & Seq
Reverse (-)
4691006 .. 4694055
3050 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G14550.3

Sequence Viewer

Length: 1134 bp
ATGAAGAAGAAGGTGTCTCAGCAGAAGTTACTGTACAGATGGAAGAGGAAGGTATACGCCACGTTGATGTTCGCTTTCTGCTTTGGGACTTTCGTATTTATACAAGCTCGTTTCGCATCTATACAAGCTCGTTTCAATCGAATCTCTGCGTCTCTCGATTCGCTTAAAAAGCCTCGTCTAGATCAGAGACCACAGATTGCCTTCCTCTTCATTGCCCGGAATCGACTCCCTCTCGAGTTTGTCTGGGATGCTTTCTTTAAGGGTGAGGATGGAAAGTTCTCAATATATGTTCATTCTAGACCTGGATTTGTTCTCAACGAGGCTACAACGCGATCCAAGTACTTTTTGGATCGGCAACTTAATGACAGTATACAGGTAGATTGGGGTGAATCAACCATGATTGAAGCAGAACGTGTATTGCTTAGACATGCACTTAGAGATTCATTTAATCACCGCTTTGTTTTTCTTTCTGATAGCTGCATACCTCTGTACAGTTTCAGCTACACGTATAACTACATCATGTCAACACCAACTAGTTTCGTTGATAGCTTTGCAGATACAAAAGATAGCCGTTATAATCCTAGAATGAATCCCATTATTCCTGTTCGTAACTGGAGAAAAGGATCACAGTGGGTCGTTCTGAATAGAAAACACGCAGAAATTGTGGTGAATGATACCTCTGTCTTTCCTATGTTTCAGCAGCATTGCAGGAGAAAATCACTTCCAGAGTTTTGGCGAGATCGTCCTGTACCAGCTGAAGGTTGGAAGGAACACAACTGTATACCTGATGAGCACTATGTTCAGACATTGCTATCTCAAAAGGGTGTAGATAGCGAACTCACACGAAGATCACTGACACACTCAGCTTGGGACCTTTCATCCTCGAAAAGTAATGAACGTCGTGGATGGCATCCTATGACTTACAAGTTTTCTGATGCTACTCCTGATCTTATACAGTCCATTAAGGGAATCGACAATATCAACTACGAGACTGAATACCGGCGAGAATGGTGTAGCAGTAAAGGGAAACCATCACCGTGCTTCCTCTTCGCCAGGAAGTTCACTCGTCCCGCCGCTCTCCGCCTACTCCGTGAAACTATCTTGTTAGAGGGCAAAGAGCATGACAATAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

377

Amino Acids

44.55

Weight (kDa)

9.75

Isoelectric Point (pI)

41.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 66 - 321 1.2e-74 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 576
AccBSI CCGCTC 1 cut(s) 1076
AccI GTMKAC 3 cut(s) 54, 370, 781
AccII CGCG 1 cut(s) 331
AciI CCGC 4 cut(s) 454, 1071, 1074, 1081
AclWI GGATC 3 cut(s) 327, 357, 631
AcuI CTGAAG 1 cut(s) 777
AfaI GTAC 4 cut(s) 35, 341, 491, 750
AfiI CCNNNNNNNGG 1 cut(s) 758
AflIII ACRYGT 2 cut(s) 412, 504
AgsI TTSAA 2 cut(s) 136, 404
AhlI ACTAGT 1 cut(s) 533
AjnI CCWGG 2 cut(s) 301, 1052
AloI GAACNNNNNNTCC 2 cut(s) 260, 292
AluBI AGCT 7 cut(s) 107, 128, 477, 501, 549, 755, 866
AluI AGCT 7 cut(s) 107, 128, 477, 501, 549, 755, 866
Alw21I GWGCWC 1 cut(s) 795
Alw26I GTCTC 4 cut(s) 21, 156, 181, 983
AlwI GGATC 3 cut(s) 327, 357, 631
Ama87I CYCGRG 1 cut(s) 233
ApeKI GCWGC 2 cut(s) 477, 700
AspS9I GGNCC 1 cut(s) 871
AsuC2I CCSGG 1 cut(s) 217
AsuHPI GGTGA 5 cut(s) 275, 398, 443, 679, 1026
AvaI CYCGRG 1 cut(s) 233
AvaII GGWCC 1 cut(s) 871
BaeI ACNNNNGTAYC 2 cut(s) 666, 699
BarI GAAGNNNNNNTAC 2 cut(s) 17, 49
Bbv12I GWGCWC 1 cut(s) 795
BbvI GCAGC 2 cut(s) 464, 712
BccI CCATC 4 cut(s) 33, 263, 900, 1039
BceAI ACGGC 1 cut(s) 555
BciT130I CCWGG 2 cut(s) 303, 1054
BcnI CCSGG 1 cut(s) 217
BcoDI GTCTC 4 cut(s) 21, 156, 181, 983
BcuI ACTAGT 1 cut(s) 533
BfaI CTAG 4 cut(s) 179, 297, 534, 582
BisI GCNGC 3 cut(s) 478, 701, 1074
BlsI GCNGC 3 cut(s) 479, 702, 1075
BmcAI AGTACT 1 cut(s) 341
Bme1390I CCNGG 3 cut(s) 217, 303, 1054
Bme18I GGWCC 1 cut(s) 871
BmeT110I CYCGRG 1 cut(s) 233
BmgT120I GGNCC 1 cut(s) 871
BmiI GGNNCC 1 cut(s) 872
BmrFI CCNGG 3 cut(s) 217, 303, 1054
BmsI GCATC 4 cut(s) 125, 238, 919, 925
BpmI CTGGAG 1 cut(s) 634
BpuMI CCSGG 1 cut(s) 217
BsaAI YACGTR 1 cut(s) 507
BsaI GGTCTC 1 cut(s) 181
Bsc4I CCNNNNNNNGG 1 cut(s) 758
Bse118I RCCGGY 1 cut(s) 999
Bse1I ACTGG 1 cut(s) 617
Bse3DI GCAATG 3 cut(s) 210, 703, 806
BseBI CCWGG 2 cut(s) 303, 1054
BseGI GGATG 5 cut(s) 253, 274, 878, 910, 911
BseLI CCNNNNNNNGG 1 cut(s) 758
BseMI GCAATG 3 cut(s) 210, 703, 806
BseMII CTCAG 2 cut(s) 32, 876
BseNI ACTGG 1 cut(s) 617
BseXI GCAGC 2 cut(s) 464, 712
Bsh1236I CGCG 1 cut(s) 331
BsiHKAI GWGCWC 1 cut(s) 795
BsiHKCI CYCGRG 1 cut(s) 233
BsiSI CCGG 2 cut(s) 217, 1000
BslFI GGGAC 3 cut(s) 100, 884, 1053
BslI CCNNNNNNNGG 1 cut(s) 758
BsmAI GTCTC 4 cut(s) 21, 156, 181, 983
BsmBI CGTCTC 1 cut(s) 156
BsmFI GGGAC 3 cut(s) 100, 884, 1053
Bso31I GGTCTC 1 cut(s) 181
BsoBI CYCGRG 1 cut(s) 233
Bsp1286I GDGCHC 1 cut(s) 795
Bsp1407I TGTACA 2 cut(s) 33, 489
Bsp143I GATC 7 cut(s) 181, 332, 349, 623, 739, 848, 946
BspACI CCGC 4 cut(s) 454, 1071, 1074, 1081
BspCNI CTCAG 2 cut(s) 31, 875
BspFNI CGCG 1 cut(s) 331
BspLI GGNNCC 1 cut(s) 872
BspPI GGATC 3 cut(s) 327, 357, 631
BspTNI GGTCTC 1 cut(s) 181
BsrBI CCGCTC 1 cut(s) 1076
BsrDI GCAATG 3 cut(s) 210, 703, 806
BsrFI RCCGGY 1 cut(s) 999
BsrGI TGTACA 2 cut(s) 33, 489
BsrI ACTGG 1 cut(s) 617
BssAI RCCGGY 1 cut(s) 999
BssMI GATC 7 cut(s) 181, 332, 349, 623, 739, 848, 946
BssNAI GTATAC 3 cut(s) 55, 371, 782
Bst1107I GTATAC 3 cut(s) 55, 371, 782
Bst2UI CCWGG 2 cut(s) 303, 1054
Bst4CI ACNGT 7 cut(s) 33, 368, 494, 630, 779, 957, 1038
Bst6I CTCTTC 3 cut(s) 38, 212, 1052
BstAUI TGTACA 2 cut(s) 33, 489
BstBAI YACGTR 1 cut(s) 507
BstDEI CTNAG 4 cut(s) 18, 422, 434, 862
BstF5I GGATG 5 cut(s) 253, 274, 878, 910, 911
BstFNI CGCG 1 cut(s) 331
BstKTI GATC 7 cut(s) 184, 335, 352, 626, 742, 851, 949
BstMAI GTCTC 4 cut(s) 21, 156, 181, 983
BstMBI GATC 7 cut(s) 181, 332, 349, 623, 739, 848, 946
BstMWI GCNNNNNNNGC 2 cut(s) 113, 169
BstNI CCWGG 2 cut(s) 303, 1054
BstNSI RCATGY 1 cut(s) 431
BstSCI CCNGG 3 cut(s) 215, 301, 1052
BstUI CGCG 1 cut(s) 331
BstV1I GCAGC 2 cut(s) 464, 712
BstXI CCANNNNNNTGG 1 cut(s) 732
BstZ17I GTATAC 3 cut(s) 55, 371, 782
BtsCI GGATG 5 cut(s) 253, 274, 878, 910, 911
BtsIMutI CAGTG 2 cut(s) 635, 851
Cfr10I RCCGGY 1 cut(s) 999
Cfr13I GGNCC 1 cut(s) 871
CseI GACGC 1 cut(s) 138
Csp6I GTAC 4 cut(s) 34, 340, 490, 749
CviAII CATG 4 cut(s) 397, 428, 520, 1121
CviQI GTAC 4 cut(s) 34, 340, 490, 749
DdeI CTNAG 4 cut(s) 18, 422, 434, 862
DpnI GATC 7 cut(s) 183, 334, 351, 625, 741, 850, 948
DpnII GATC 7 cut(s) 181, 332, 349, 623, 739, 848, 946
Eam1104I CTCTTC 3 cut(s) 38, 212, 1052
EarI CTCTTC 3 cut(s) 38, 212, 1052
EciI GGCGGA 1 cut(s) 1070
Eco31I GGTCTC 1 cut(s) 181
Eco47I GGWCC 1 cut(s) 871
Eco57I CTGAAG 1 cut(s) 777
Eco88I CYCGRG 1 cut(s) 233
EcoO109I RGGNCCY 1 cut(s) 871
EcoRII CCWGG 2 cut(s) 301, 1052
Esp3I CGTCTC 1 cut(s) 156
FaeI CATG 4 cut(s) 400, 431, 523, 1124
FaqI GGGAC 3 cut(s) 100, 884, 1053
FatI CATG 4 cut(s) 396, 427, 519, 1120
FauI CCCGC 1 cut(s) 1078
FblI GTMKAC 3 cut(s) 54, 370, 781
Fnu4HI GCNGC 3 cut(s) 478, 701, 1074
FokI GGATG 5 cut(s) 260, 281, 865, 897, 918
Fsp4HI GCNGC 3 cut(s) 478, 701, 1074
FspBI CTAG 4 cut(s) 179, 297, 534, 582
GluI GCNGC 3 cut(s) 478, 701, 1074
GsuI CTGGAG 1 cut(s) 634
HapII CCGG 2 cut(s) 217, 1000
HgaI GACGC 1 cut(s) 138
Hin1II CATG 4 cut(s) 400, 431, 523, 1124
HincII GTYRAC 1 cut(s) 525
HindII GTYRAC 1 cut(s) 525
HinfI GANTC 8 cut(s) 141, 158, 220, 225, 389, 440, 589, 969
HpaII CCGG 2 cut(s) 217, 1000
HphI GGTGA 5 cut(s) 275, 398, 443, 679, 1026
Hpy166II GTNNAC 5 cut(s) 55, 371, 525, 782, 1062
Hpy188I TCNGA 5 cut(s) 186, 472, 642, 804, 934
Hpy188III TCNNGA 6 cut(s) 155, 179, 233, 297, 725, 944
Hpy8I GTNNAC 5 cut(s) 55, 371, 525, 782, 1062
Hpy99I CGWCG 1 cut(s) 903
HpyAV CCTTC 5 cut(s) 4, 43, 211, 752, 760
HpyCH4III ACNGT 7 cut(s) 33, 368, 494, 630, 779, 957, 1038
HpyCH4IV ACGT 4 cut(s) 62, 412, 506, 898
HpyCH4V TGCA 4 cut(s) 431, 480, 554, 708
HpyF10VI GCNNNNNNNGC 2 cut(s) 113, 169
HpyF3I CTNAG 4 cut(s) 18, 422, 434, 862
HpySE526I ACGT 4 cut(s) 62, 412, 506, 898
Hsp92II CATG 4 cut(s) 400, 431, 523, 1124
Kzo9I GATC 7 cut(s) 181, 332, 349, 623, 739, 848, 946
Lsp1109I GCAGC 2 cut(s) 464, 712
LweI GCATC 4 cut(s) 125, 238, 919, 925
MaeI CTAG 4 cut(s) 179, 297, 534, 582
MaeII ACGT 4 cut(s) 62, 412, 506, 898
MaeIII GTNAC 2 cut(s) 27, 608
MalI GATC 7 cut(s) 183, 334, 351, 625, 741, 850, 948
MbiI CCGCTC 1 cut(s) 1076
MboI GATC 7 cut(s) 181, 332, 349, 623, 739, 848, 946
MboII GAAGA 6 cut(s) 16, 19, 55, 199, 858, 1039
MhlI GDGCHC 1 cut(s) 795
MluCI AATT 1 cut(s) 660
MlyI GAGTC 1 cut(s) 219
MmeI TCCRAC 1 cut(s) 743
MseI TTAA 5 cut(s) 165, 258, 360, 447, 963
MslI CAYNNNNRTG 2 cut(s) 65, 1036
MspA1I CMGCKG 1 cut(s) 755
MspI CCGG 2 cut(s) 217, 1000
MspR9I CCNGG 3 cut(s) 217, 303, 1054
MvaI CCWGG 2 cut(s) 303, 1054
MvnI CGCG 1 cut(s) 331
MwoI GCNNNNNNNGC 2 cut(s) 113, 169
NciI CCSGG 1 cut(s) 217
NdeII GATC 7 cut(s) 181, 332, 349, 623, 739, 848, 946
NlaIII CATG 4 cut(s) 400, 431, 523, 1124
NlaIV GGNNCC 1 cut(s) 872
NspI RCATGY 1 cut(s) 431
PaeR7I CTCGAG 1 cut(s) 233
PcsI WCGNNNNNNNCGW 1 cut(s) 136
PfeI GAWTC 7 cut(s) 141, 158, 220, 389, 440, 589, 969
PkrI GCNGC 3 cut(s) 479, 702, 1075
PleI GAGTC 1 cut(s) 219
PpsI GAGTC 1 cut(s) 219
Ppu21I YACGTR 1 cut(s) 507
PpuMI RGGWCCY 1 cut(s) 871
PsiI TTATAA 1 cut(s) 576
Psp5II RGGWCCY 1 cut(s) 871
Psp6I CCWGG 2 cut(s) 301, 1052
PspGI CCWGG 2 cut(s) 301, 1052
PspN4I GGNNCC 1 cut(s) 872
PspPI GGNCC 1 cut(s) 871
PspPPI RGGWCCY 1 cut(s) 871
PvuII CAGCTG 1 cut(s) 755
RsaI GTAC 4 cut(s) 35, 341, 491, 750
RsaNI GTAC 4 cut(s) 34, 340, 490, 749
RseI CAYNNNNRTG 2 cut(s) 65, 1036
SaqAI TTAA 5 cut(s) 165, 258, 360, 447, 963
SatI GCNGC 3 cut(s) 478, 701, 1074
Sau3AI GATC 7 cut(s) 181, 332, 349, 623, 739, 848, 946
Sau96I GGNCC 1 cut(s) 871
ScaI AGTACT 1 cut(s) 341
SchI GAGTC 1 cut(s) 219
ScrFI CCNGG 3 cut(s) 217, 303, 1054
SduI GDGCHC 1 cut(s) 795
SfaNI GCATC 4 cut(s) 125, 238, 919, 925
Sfr274I CTCGAG 1 cut(s) 233
SinI GGWCC 1 cut(s) 871
SlaI CTCGAG 1 cut(s) 233
SmiMI CAYNNNNRTG 2 cut(s) 65, 1036
SmlI CTYRAG 1 cut(s) 233
SmoI CTYRAG 1 cut(s) 233
SpeI ACTAGT 1 cut(s) 533
Sse9I AATT 1 cut(s) 660
SsiI CCGC 4 cut(s) 454, 1071, 1074, 1081
SspMI CTAG 4 cut(s) 179, 297, 534, 582
StyD4I CCNGG 3 cut(s) 215, 301, 1052
TaaI ACNGT 7 cut(s) 33, 368, 494, 630, 779, 957, 1038
TaiI ACGT 4 cut(s) 65, 415, 509, 901
TaqI TCGA 6 cut(s) 139, 156, 223, 234, 884, 972
TasI AATT 1 cut(s) 660
TatI WGTACW 3 cut(s) 33, 339, 489
TauI GCSGC 1 cut(s) 1076
TfiI GAWTC 7 cut(s) 141, 158, 220, 389, 440, 589, 969
Tru1I TTAA 5 cut(s) 165, 258, 360, 447, 963
Tru9I TTAA 5 cut(s) 165, 258, 360, 447, 963
TscAI CASTG 2 cut(s) 635, 858
TseI GCWGC 2 cut(s) 477, 700
TspDTI ATGAA 7 cut(s) 17, 199, 281, 432, 602, 867, 909
TspGWI ACGGA 1 cut(s) 1079
TspRI CASTG 2 cut(s) 635, 858
VpaK11BI GGWCC 1 cut(s) 871
XbaI TCTAGA 2 cut(s) 178, 296
XceI RCATGY 1 cut(s) 431
XcmI CCANNNNNNNNNTGG 2 cut(s) 343, 759
XhoI CTCGAG 1 cut(s) 233
XmiI GTMKAC 3 cut(s) 54, 370, 781
XspI CTAG 4 cut(s) 179, 297, 534, 582
ZrmI AGTACT 1 cut(s) 341
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.