MD17G1030200.v1.1

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0002928
Physical Location & Seq
Reverse (-)
2187350 .. 2191607
4258 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1030200.v1.1.491

Sequence Viewer

Length: 1107 bp
ATGAAGCGGAAGGCGGGTCCGCAGAAAGCTCAGCACAAATGGAAGAGGAAGCTGTTCACTTTGATTTTGGTGGGGATCTGCTTCGGGACGTTGGTGCTGCTGATGCACACTCAGTACAGTCGGATTATGACGCTCGCTTCGTTGCGAAATTCTCAGCAAAATCAACGGCCCAAGATCGCCTTCTTGTTCATCGCTCGCAATCGCATTCCGTTGGACATTCTTTGGGATGTTTTCTTTCAGGGAGGGGAGAGTAGGTTTTCGATTTATGTTCACTCAAGGCCGGGGTTTCTGTTCAACAAGGCCACCACAAGGTCGGTTTTTTTCTTGGATCGTCAAGTCAATGATAGTATACAGGTAGATTGGGGAGAAGCAAGCATGATTGAGGCAGAGCGCATATTACTAAGACATGCACTCGAGGATCCTCTTAATCAACGCTTTGCCTTTGTTTCCGATAGCTGCATTCCCCTTTACAATTTCAGCTACATATATGACTACATCATGTCGACGAGAACCAGTTTTGTAGACAGCTTTGCTGATACGAAAGAGGGCCGCTACAATCCAAAAATGCATCCTGTTATTCCTGTTCATAACTGGAGAAAAGGATCACAGTGGGTTGTCTTGACCAGGAAGCATGCAGAGGTCGTAGTGAAAGACGATAGAGTCTTTCCTATGTTTCAATTGCATTGCAAGACAAAATCGCTACCTGAGTTTTGGCGGAATCGTCCCATTCCTGCTGATACATCCAAGCAGCACAATTGTATCCCGGACGAGCATTATGTTCAGACATTACTGGCTCAACAAGGCCTTGAGGGAGAACTCACGCGTAGATCGCTGACACACACGTCATGGGATTTATCATCCTCTAGAAACCGTGAACGCCGTGGATGGCACCCTTTGACTTACAAATTTTCAGATGCTACTCCCATGCTTATTAAATCTATAAAGGAGATAGATAATGTCTACTACGAGACTGAATACCGGAGAGAATGGTGTAGCAGCAAGGGAAAACCATCTCGGTGCTTTCTTTTTGCTCGAAAATTTACCCGTCCTGCTGCTCTCAGGCTTCTTAACACGACTGCACTGGAACTTAACCGGAGCAATGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

369

Amino Acids

43.32

Weight (kDa)

9.87

Isoelectric Point (pI)

44.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 59 - 314 1.6e-70 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 659, 841
AccB1I GGYRCC 1 cut(s) 888
AccI GTMKAC 4 cut(s) 349, 503, 522, 960
AccII CGCG 1 cut(s) 823
AciI CCGC 5 cut(s) 7, 14, 20, 550, 715
AclWI GGATC 5 cut(s) 83, 336, 413, 426, 610
AcsI RAATTY 3 cut(s) 148, 905, 1037
AfaI GTAC 1 cut(s) 116
AfiI CCNNNNNNNGG 1 cut(s) 309
AflIII ACRYGT 2 cut(s) 821, 840
AgsI TTSAA 2 cut(s) 295, 677
AjiI CACGTC 1 cut(s) 843
AjnI CCWGG 1 cut(s) 623
AjuI GAANNNNNNNTTGG 2 cut(s) 164, 196
AluBI AGCT 5 cut(s) 29, 52, 456, 480, 528
AluI AGCT 5 cut(s) 29, 52, 456, 480, 528
Alw26I GTCTC 1 cut(s) 962
AlwI GGATC 5 cut(s) 83, 336, 413, 426, 610
Ama87I CYCGRG 1 cut(s) 413
AoxI GGCC 5 cut(s) 167, 278, 300, 547, 802
ApeKI GCWGC 5 cut(s) 97, 456, 748, 996, 1052
ApoI RAATTY 3 cut(s) 148, 905, 1037
ArsI GACNNNNNNTTYG 2 cut(s) 121, 153
Asp700I GAANNNNTTC 1 cut(s) 53
AspLEI GCGC 1 cut(s) 393
AspS9I GGNCC 3 cut(s) 17, 168, 547
AsuC2I CCSGG 2 cut(s) 282, 764
AvaI CYCGRG 1 cut(s) 413
AvaII GGWCC 1 cut(s) 17
BaeI ACNNNNGTAYC 2 cut(s) 742, 775
BamHI GGATCC 1 cut(s) 418
BanI GGYRCC 1 cut(s) 888
BbvI GCAGC 5 cut(s) 84, 443, 760, 1008, 1039
BccI CCATC 2 cut(s) 879, 1018
BceAI ACGGC 2 cut(s) 182, 864
BciT130I CCWGG 1 cut(s) 625
BciVI GTATCC 1 cut(s) 770
BcnI CCSGG 2 cut(s) 282, 764
BcoDI GTCTC 1 cut(s) 962
BfaI CTAG 1 cut(s) 864
BfuI GTATCC 1 cut(s) 770
BisI GCNGC 6 cut(s) 98, 457, 550, 749, 997, 1053
BlpI GCTNAGC 1 cut(s) 30
BlsI GCNGC 6 cut(s) 99, 458, 551, 750, 998, 1054
Bme1390I CCNGG 3 cut(s) 282, 625, 764
Bme18I GGWCC 1 cut(s) 17
BmeT110I CYCGRG 1 cut(s) 413
BmgBI CACGTC 1 cut(s) 843
BmgT120I GGNCC 3 cut(s) 17, 168, 547
BmiI GGNNCC 3 cut(s) 18, 420, 890
BmrFI CCNGG 3 cut(s) 282, 625, 764
BmsI GCATC 3 cut(s) 93, 577, 904
BpmI CTGGAG 1 cut(s) 613
Bpu1102I GCTNAGC 1 cut(s) 30
BpuEI CTTGAG 2 cut(s) 259, 827
BpuMI CCSGG 2 cut(s) 282, 764
BsaJI CCNNGG 2 cut(s) 281, 880
BsaWI WCCGGW 2 cut(s) 978, 1092
BsaXI ACNNNNNCTCC 2 cut(s) 239, 269
Bsc4I CCNNNNNNNGG 1 cut(s) 309
Bse1I ACTGG 4 cut(s) 513, 596, 795, 1086
Bse3DI GCAATG 2 cut(s) 682, 1105
BseBI CCWGG 1 cut(s) 625
BseDI CCNNGG 2 cut(s) 281, 880
BseGI GGATG 5 cut(s) 232, 568, 740, 857, 890
BseLI CCNNNNNNNGG 1 cut(s) 309
BseMI GCAATG 2 cut(s) 682, 1105
BseMII CTCAG 5 cut(s) 44, 125, 167, 696, 1072
BseNI ACTGG 4 cut(s) 513, 596, 795, 1086
BseXI GCAGC 5 cut(s) 84, 443, 760, 1008, 1039
BsgI GTGCAG 1 cut(s) 1062
Bsh1236I CGCG 1 cut(s) 823
BshFI GGCC 5 cut(s) 169, 280, 302, 549, 804
BshNI GGYRCC 1 cut(s) 888
BsiHKCI CYCGRG 1 cut(s) 413
BsiSI CCGG 4 cut(s) 281, 764, 979, 1093
BslFI GGGAC 2 cut(s) 100, 708
BslI CCNNNNNNNGG 1 cut(s) 309
BsmAI GTCTC 1 cut(s) 962
BsmFI GGGAC 2 cut(s) 100, 708
BsmI GAATGC 2 cut(s) 204, 459
BsnI GGCC 5 cut(s) 169, 280, 302, 549, 804
BsoBI CYCGRG 1 cut(s) 413
Bsp143I GATC 6 cut(s) 75, 174, 328, 418, 602, 827
Bsp1720I GCTNAGC 1 cut(s) 30
BspACI CCGC 5 cut(s) 7, 14, 20, 550, 715
BspANI GGCC 5 cut(s) 169, 280, 302, 549, 804
BspCNI CTCAG 5 cut(s) 43, 124, 166, 697, 1071
BspFNI CGCG 1 cut(s) 823
BspLI GGNNCC 3 cut(s) 18, 420, 890
BspPI GGATC 5 cut(s) 83, 336, 413, 426, 610
BspT107I GGYRCC 1 cut(s) 888
BsrDI GCAATG 2 cut(s) 682, 1105
BsrI ACTGG 4 cut(s) 513, 596, 795, 1086
BssECI CCNNGG 2 cut(s) 281, 880
BssMI GATC 6 cut(s) 75, 174, 328, 418, 602, 827
BssNAI GTATAC 1 cut(s) 350
Bst1107I GTATAC 1 cut(s) 350
Bst2UI CCWGG 1 cut(s) 625
Bst4CI ACNGT 3 cut(s) 119, 609, 872
Bst6I CTCTTC 1 cut(s) 38
BstC8I GCNNGC 4 cut(s) 135, 196, 373, 633
BstDEI CTNAG 6 cut(s) 30, 111, 153, 401, 705, 1058
BstDSI CCRYGG 1 cut(s) 880
BstF5I GGATG 5 cut(s) 232, 568, 740, 857, 890
BstFNI CGCG 1 cut(s) 823
BstHHI GCGC 1 cut(s) 393
BstKTI GATC 6 cut(s) 78, 177, 331, 421, 605, 830
BstMAI GTCTC 1 cut(s) 962
BstMBI GATC 6 cut(s) 75, 174, 328, 418, 602, 827
BstMWI GCNNNNNNNGC 2 cut(s) 103, 829
BstNI CCWGG 1 cut(s) 625
BstNSI RCATGY 2 cut(s) 410, 635
BstSCI CCNGG 3 cut(s) 280, 623, 762
BstUI CGCG 1 cut(s) 823
BstV1I GCAGC 5 cut(s) 84, 443, 760, 1008, 1039
BstX2I RGATCY 2 cut(s) 75, 418
BstYI RGATCY 2 cut(s) 75, 418
BstZ17I GTATAC 1 cut(s) 350
BsuI GTATCC 1 cut(s) 770
BsuRI GGCC 5 cut(s) 169, 280, 302, 549, 804
BtgI CCRYGG 1 cut(s) 880
BtgZI GCGATG 1 cut(s) 175
BtrI CACGTC 1 cut(s) 843
BtsCI GGATG 5 cut(s) 232, 568, 740, 857, 890
BtsIMutI CAGTG 2 cut(s) 614, 1079
Cac8I GCNNGC 4 cut(s) 135, 196, 373, 633
CfoI GCGC 1 cut(s) 393
Cfr13I GGNCC 3 cut(s) 17, 168, 547
CseI GACGC 1 cut(s) 139
Csp6I GTAC 1 cut(s) 115
CviAII CATG 6 cut(s) 376, 407, 499, 632, 846, 925
CviQI GTAC 1 cut(s) 115
DdeI CTNAG 6 cut(s) 30, 111, 153, 401, 705, 1058
DpnI GATC 6 cut(s) 77, 176, 330, 420, 604, 829
DpnII GATC 6 cut(s) 75, 174, 328, 418, 602, 827
DrdI GACNNNNNNGTC 2 cut(s) 659, 841
DseDI GACNNNNNNGTC 2 cut(s) 659, 841
Eam1104I CTCTTC 1 cut(s) 38
EarI CTCTTC 1 cut(s) 38
EciI GGCGGA 1 cut(s) 730
Eco147I AGGCCT 1 cut(s) 804
Eco47I GGWCC 1 cut(s) 17
Eco88I CYCGRG 1 cut(s) 413
EcoRII CCWGG 1 cut(s) 623
EcoT22I ATGCAT 1 cut(s) 570
FaeI CATG 6 cut(s) 379, 410, 502, 635, 849, 928
FalI AAGNNNNNCTT 2 cut(s) 164, 196
FaqI GGGAC 2 cut(s) 100, 708
FatI CATG 6 cut(s) 375, 406, 498, 631, 845, 924
FauI CCCGC 1 cut(s) 7
FblI GTMKAC 4 cut(s) 349, 503, 522, 960
Fnu4HI GCNGC 6 cut(s) 98, 457, 550, 749, 997, 1053
FokI GGATG 5 cut(s) 239, 555, 727, 844, 897
Fsp4HI GCNGC 6 cut(s) 98, 457, 550, 749, 997, 1053
FspBI CTAG 1 cut(s) 864
GlaI GCGC 1 cut(s) 392
GluI GCNGC 6 cut(s) 98, 457, 550, 749, 997, 1053
GsuI CTGGAG 1 cut(s) 613
HaeIII GGCC 5 cut(s) 169, 280, 302, 549, 804
HapII CCGG 4 cut(s) 281, 764, 979, 1093
HgaI GACGC 1 cut(s) 139
HhaI GCGC 1 cut(s) 393
Hin1II CATG 6 cut(s) 379, 410, 502, 635, 849, 928
Hin6I GCGC 1 cut(s) 391
HinP1I GCGC 1 cut(s) 391
HincII GTYRAC 1 cut(s) 504
HindII GTYRAC 1 cut(s) 504
HinfI GANTC 2 cut(s) 660, 718
HpaII CCGG 4 cut(s) 281, 764, 979, 1093
Hpy166II GTNNAC 7 cut(s) 57, 271, 350, 504, 523, 875, 961
Hpy188I TCNGA 4 cut(s) 123, 451, 783, 913
Hpy188III TCNNGA 3 cut(s) 85, 619, 864
Hpy8I GTNNAC 7 cut(s) 57, 271, 350, 504, 523, 875, 961
Hpy99I CGWCG 1 cut(s) 508
HpyAV CCTTC 2 cut(s) 4, 190
HpyCH4III ACNGT 3 cut(s) 119, 609, 872
HpyCH4IV ACGT 2 cut(s) 89, 842
HpyCH4V TGCA 8 cut(s) 106, 410, 459, 568, 635, 682, 687, 1079
HpyF10VI GCNNNNNNNGC 2 cut(s) 103, 829
HpyF3I CTNAG 6 cut(s) 30, 111, 153, 401, 705, 1058
HpySE526I ACGT 2 cut(s) 89, 842
Hsp92II CATG 6 cut(s) 379, 410, 502, 635, 849, 928
HspAI GCGC 1 cut(s) 391
Kzo9I GATC 6 cut(s) 75, 174, 328, 418, 602, 827
LmnI GCTCC 1 cut(s) 1095
Lsp1109I GCAGC 5 cut(s) 84, 443, 760, 1008, 1039
LweI GCATC 3 cut(s) 93, 577, 904
MaeI CTAG 1 cut(s) 864
MaeII ACGT 2 cut(s) 89, 842
MalI GATC 6 cut(s) 77, 176, 330, 420, 604, 829
MboI GATC 6 cut(s) 75, 174, 328, 418, 602, 827
MboII GAAGA 1 cut(s) 55
MfeI CAATTG 2 cut(s) 677, 754
MflI RGATCY 2 cut(s) 75, 418
MluCI AATT 6 cut(s) 148, 472, 677, 754, 905, 1037
MluI ACGCGT 1 cut(s) 821
MlyI GAGTC 1 cut(s) 669
MmeI TCCRAC 2 cut(s) 101, 192
MnlI CCTC 9 cut(s) 39, 236, 376, 409, 432, 538, 631, 802, 871
Mph1103I ATGCAT 1 cut(s) 570
MroXI GAANNNNTTC 1 cut(s) 53
MseI TTAA 5 cut(s) 426, 933, 1068, 1089, 1105
MslI CAYNNNNRTG 1 cut(s) 1015
MspI CCGG 4 cut(s) 281, 764, 979, 1093
MspR9I CCNGG 3 cut(s) 282, 625, 764
MunI CAATTG 2 cut(s) 677, 754
Mva1269I GAATGC 2 cut(s) 204, 459
MvaI CCWGG 1 cut(s) 625
MvnI CGCG 1 cut(s) 823
MwoI GCNNNNNNNGC 2 cut(s) 103, 829
NciI CCSGG 2 cut(s) 282, 764
NdeII GATC 6 cut(s) 75, 174, 328, 418, 602, 827
NlaIII CATG 6 cut(s) 379, 410, 502, 635, 849, 928
NlaIV GGNNCC 3 cut(s) 18, 420, 890
NsiI ATGCAT 1 cut(s) 570
NspI RCATGY 2 cut(s) 410, 635
PaeI GCATGC 1 cut(s) 635
PaeR7I CTCGAG 1 cut(s) 413
PceI AGGCCT 1 cut(s) 804
PcsI WCGNNNNNNNCGW 1 cut(s) 137
PctI GAATGC 2 cut(s) 204, 459
PdmI GAANNNNTTC 1 cut(s) 53
PfeI GAWTC 1 cut(s) 718
PfoI TCCNGGA 1 cut(s) 762
PkrI GCNGC 6 cut(s) 99, 458, 551, 750, 998, 1054
PleI GAGTC 1 cut(s) 668
PpsI GAGTC 1 cut(s) 668
Psp6I CCWGG 1 cut(s) 623
PspGI CCWGG 1 cut(s) 623
PspN4I GGNNCC 3 cut(s) 18, 420, 890
PspPI GGNCC 3 cut(s) 17, 168, 547
PspXI VCTCGAGB 1 cut(s) 413
PsuI RGATCY 2 cut(s) 75, 418
RsaI GTAC 1 cut(s) 116
RsaNI GTAC 1 cut(s) 115
RseI CAYNNNNRTG 1 cut(s) 1015
SalI GTCGAC 1 cut(s) 502
SaqAI TTAA 5 cut(s) 426, 933, 1068, 1089, 1105
SatI GCNGC 6 cut(s) 98, 457, 550, 749, 997, 1053
Sau3AI GATC 6 cut(s) 75, 174, 328, 418, 602, 827
Sau96I GGNCC 3 cut(s) 17, 168, 547
SchI GAGTC 1 cut(s) 669
ScrFI CCNGG 3 cut(s) 282, 625, 764
SfaNI GCATC 3 cut(s) 93, 577, 904
Sfr274I CTCGAG 1 cut(s) 413
SinI GGWCC 1 cut(s) 17
SlaI CTCGAG 1 cut(s) 413
SmiMI CAYNNNNRTG 1 cut(s) 1015
SmlI CTYRAG 3 cut(s) 274, 413, 806
SmoI CTYRAG 3 cut(s) 274, 413, 806
SphI GCATGC 1 cut(s) 635
Sse9I AATT 6 cut(s) 148, 472, 677, 754, 905, 1037
SseBI AGGCCT 1 cut(s) 804
SsiI CCGC 5 cut(s) 7, 14, 20, 550, 715
SspMI CTAG 1 cut(s) 864
StuI AGGCCT 1 cut(s) 804
StyD4I CCNGG 3 cut(s) 280, 623, 762
TaaI ACNGT 3 cut(s) 119, 609, 872
TaiI ACGT 2 cut(s) 92, 845
TaqI TCGA 4 cut(s) 260, 414, 503, 1033
TasI AATT 6 cut(s) 148, 472, 677, 754, 905, 1037
TatI WGTACW 1 cut(s) 114
TauI GCSGC 1 cut(s) 552
TfiI GAWTC 1 cut(s) 718
Tru1I TTAA 5 cut(s) 426, 933, 1068, 1089, 1105
Tru9I TTAA 5 cut(s) 426, 933, 1068, 1089, 1105
TscAI CASTG 2 cut(s) 614, 1086
TseI GCWGC 5 cut(s) 97, 456, 748, 996, 1052
TspDTI ATGAA 3 cut(s) 17, 178, 575
TspGWI ACGGA 1 cut(s) 198
TspRI CASTG 2 cut(s) 614, 1086
VpaK11BI GGWCC 1 cut(s) 17
XapI RAATTY 3 cut(s) 148, 905, 1037
XbaI TCTAGA 1 cut(s) 863
XceI RCATGY 2 cut(s) 410, 635
XhoI CTCGAG 1 cut(s) 413
XmiI GTMKAC 4 cut(s) 349, 503, 522, 960
XmnI GAANNNNTTC 1 cut(s) 53
XspI CTAG 1 cut(s) 864
Zsp2I ATGCAT 1 cut(s) 570
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.