Rh7AG291800

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0002928
Physical Location & Seq
Reverse (-)
31834954 .. 31839167
4214 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG291800.1

Sequence Viewer

Length: 450 bp
ATGTCAACCAGAACCAGTTTTGTAGACAGCTTTGCTGATACAAAAGATGGCCGCTACAATCCAAAAATGGATCCTGTTATTCCTGTACAAAACTGGAGAAAAGGATCTCAGTGGGTTGTATTGACGAGGAAGCATGCAGAGGTTGTAGTGAAAGACGATACAGTGTTTCCTATGTTCCAGTTGTATTGTAAGAGGAAGTCGTTACCTGAGTTTTGGCGGGATCATCCTGTTCCGACCGATAACTCCAAGGATCACAATTGCATTCCAGACGAGCATTATGTTCAGACATTACTGGCTGATGTAGATAATATCTACTATGAGACCGAGTATCGAAGAGAATGGGGAGATTGTGCAAATTTGGTCTCTTTCTTTGAGGGGTCAGGTCACACAAACAAGAAGAAGAAGAAGAAGAAGCACAAGAAAGTTCTCCTGAGGCATGGCTGCAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

149

Amino Acids

17.59

Weight (kDa)

9.13

Isoelectric Point (pI)

31.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 3 - 100 3.1e-23 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 24
AciI CCGC 2 cut(s) 52, 217
AclWI GGATC 5 cut(s) 65, 78, 112, 228, 258
AcoI YGGCCR 1 cut(s) 49
AcsI RAATTY 1 cut(s) 355
AfaI GTAC 1 cut(s) 87
AluBI AGCT 1 cut(s) 30
AluI AGCT 1 cut(s) 30
Alw26I GTCTC 2 cut(s) 314, 367
AlwI GGATC 5 cut(s) 65, 78, 112, 228, 258
AoxI GGCC 1 cut(s) 49
ApeKI GCWGC 1 cut(s) 441
ApoI RAATTY 1 cut(s) 355
AxyI CCTNAGG 1 cut(s) 431
BamHI GGATCC 1 cut(s) 70
BbvI GCAGC 1 cut(s) 428
BccI CCATC 1 cut(s) 41
BcoDI GTCTC 2 cut(s) 314, 367
BisI GCNGC 2 cut(s) 52, 442
BlsI GCNGC 2 cut(s) 53, 443
BmiI GGNNCC 1 cut(s) 72
BpmI CTGGAG 1 cut(s) 115
BsaI GGTCTC 2 cut(s) 314, 367
BsaJI CCNNGG 1 cut(s) 246
Bse1I ACTGG 4 cut(s) 15, 98, 178, 297
Bse21I CCTNAGG 1 cut(s) 431
BseDI CCNNGG 1 cut(s) 246
BseGI GGATG 1 cut(s) 223
BseMII CTCAG 3 cut(s) 122, 198, 422
BseNI ACTGG 4 cut(s) 15, 98, 178, 297
BseXI GCAGC 1 cut(s) 428
Bsh1285I CGRYCG 1 cut(s) 237
BshFI GGCC 1 cut(s) 51
BsiEI CGRYCG 1 cut(s) 237
BsmAI GTCTC 2 cut(s) 314, 367
BsmI GAATGC 1 cut(s) 261
BsnI GGCC 1 cut(s) 51
Bso31I GGTCTC 2 cut(s) 314, 367
Bsp1407I TGTACA 1 cut(s) 85
Bsp143I GATC 4 cut(s) 70, 104, 220, 250
BspACI CCGC 2 cut(s) 52, 217
BspANI GGCC 1 cut(s) 51
BspCNI CTCAG 3 cut(s) 121, 199, 423
BspLI GGNNCC 1 cut(s) 72
BspPI GGATC 5 cut(s) 65, 78, 112, 228, 258
BspTNI GGTCTC 2 cut(s) 314, 367
BsrGI TGTACA 1 cut(s) 85
BsrI ACTGG 4 cut(s) 15, 98, 178, 297
BssECI CCNNGG 1 cut(s) 246
BssMI GATC 4 cut(s) 70, 104, 220, 250
BssT1I CCWWGG 1 cut(s) 246
Bst4CI ACNGT 1 cut(s) 163
Bst6I CTCTTC 1 cut(s) 328
BstAUI TGTACA 1 cut(s) 85
BstC8I GCNNGC 1 cut(s) 135
BstDEI CTNAG 3 cut(s) 108, 207, 431
BstF5I GGATG 1 cut(s) 223
BstKTI GATC 4 cut(s) 73, 107, 223, 253
BstMAI GTCTC 2 cut(s) 314, 367
BstMBI GATC 4 cut(s) 70, 104, 220, 250
BstMCI CGRYCG 1 cut(s) 237
BstNSI RCATGY 1 cut(s) 137
BstV1I GCAGC 1 cut(s) 428
BstX2I RGATCY 2 cut(s) 70, 104
BstYI RGATCY 2 cut(s) 70, 104
Bsu36I CCTNAGG 1 cut(s) 431
BsuRI GGCC 1 cut(s) 51
BtsCI GGATG 1 cut(s) 223
BtsIMutI CAGTG 2 cut(s) 116, 168
Cac8I GCNNGC 1 cut(s) 135
Csp6I GTAC 1 cut(s) 86
CviAII CATG 2 cut(s) 134, 437
CviJI RGCY 4 cut(s) 30, 51, 296, 441
CviKI_1 RGCY 4 cut(s) 30, 51, 296, 441
CviQI GTAC 1 cut(s) 86
DdeI CTNAG 3 cut(s) 108, 207, 431
DpnI GATC 4 cut(s) 72, 106, 222, 252
DpnII GATC 4 cut(s) 70, 104, 220, 250
EaeI YGGCCR 1 cut(s) 49
Eam1104I CTCTTC 1 cut(s) 328
EarI CTCTTC 1 cut(s) 328
Eco130I CCWWGG 1 cut(s) 246
Eco31I GGTCTC 2 cut(s) 314, 367
Eco81I CCTNAGG 1 cut(s) 431
EcoT14I CCWWGG 1 cut(s) 246
ErhI CCWWGG 1 cut(s) 246
FaeI CATG 2 cut(s) 137, 440
FaiI YATR 5 cut(s) 135, 173, 279, 318, 438
FatI CATG 2 cut(s) 133, 436
FauI CCCGC 1 cut(s) 210
FblI GTMKAC 1 cut(s) 24
Fnu4HI GCNGC 2 cut(s) 52, 442
FokI GGATG 1 cut(s) 210
Fsp4HI GCNGC 2 cut(s) 52, 442
GluI GCNGC 2 cut(s) 52, 442
GsuI CTGGAG 1 cut(s) 115
HaeIII GGCC 1 cut(s) 51
Hin1II CATG 2 cut(s) 137, 440
HincII GTYRAC 1 cut(s) 6
HindII GTYRAC 1 cut(s) 6
Hpy166II GTNNAC 2 cut(s) 6, 25
Hpy188I TCNGA 2 cut(s) 234, 285
Hpy188III TCNNGA 2 cut(s) 266, 430
Hpy8I GTNNAC 2 cut(s) 6, 25
HpyCH4III ACNGT 1 cut(s) 163
HpyCH4V TGCA 4 cut(s) 137, 261, 353, 444
HpyF3I CTNAG 3 cut(s) 108, 207, 431
Hsp92II CATG 2 cut(s) 137, 440
Kzo9I GATC 4 cut(s) 70, 104, 220, 250
Lsp1109I GCAGC 1 cut(s) 428
MaeIII GTNAC 2 cut(s) 201, 383
MalI GATC 4 cut(s) 72, 106, 222, 252
MboI GATC 4 cut(s) 70, 104, 220, 250
MboII GAAGA 6 cut(s) 345, 409, 412, 415, 418, 421
MfeI CAATTG 2 cut(s) 256, 445
MflI RGATCY 2 cut(s) 70, 104
MluCI AATT 3 cut(s) 256, 355, 445
MmeI TCCRAC 1 cut(s) 257
MnlI CCTC 5 cut(s) 120, 133, 186, 367, 426
MunI CAATTG 2 cut(s) 256, 445
Mva1269I GAATGC 1 cut(s) 261
NdeII GATC 4 cut(s) 70, 104, 220, 250
NlaIII CATG 2 cut(s) 137, 440
NlaIV GGNNCC 1 cut(s) 72
NmuCI GTSAC 1 cut(s) 383
NspI RCATGY 1 cut(s) 137
PaeI GCATGC 1 cut(s) 137
PctI GAATGC 1 cut(s) 261
PkrI GCNGC 2 cut(s) 53, 443
PspN4I GGNNCC 1 cut(s) 72
PsuI RGATCY 2 cut(s) 70, 104
RsaI GTAC 1 cut(s) 87
RsaNI GTAC 1 cut(s) 86
SatI GCNGC 2 cut(s) 52, 442
Sau3AI GATC 4 cut(s) 70, 104, 220, 250
SetI ASST 4 cut(s) 32, 144, 208, 385
SphI GCATGC 1 cut(s) 137
Sse9I AATT 3 cut(s) 256, 355, 445
SsiI CCGC 2 cut(s) 52, 217
StyI CCWWGG 1 cut(s) 246
TaaI ACNGT 1 cut(s) 163
TaqI TCGA 1 cut(s) 331
TaqII GACCGA 2 cut(s) 251, 338
TasI AATT 3 cut(s) 256, 355, 445
TatI WGTACW 1 cut(s) 85
TauI GCSGC 1 cut(s) 54
TscAI CASTG 2 cut(s) 116, 168
TseFI GTSAC 1 cut(s) 383
TseI GCWGC 1 cut(s) 441
Tsp45I GTSAC 1 cut(s) 383
TspRI CASTG 2 cut(s) 116, 168
XapI RAATTY 1 cut(s) 355
XceI RCATGY 1 cut(s) 137
XmiI GTMKAC 1 cut(s) 24
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.