AT1G50240

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0004502
Physical Location & Seq
Forward (+)
18606511 .. 18614500
7990 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G50240.2

Sequence Viewer

Length: 3969 bp
ATGGGTGTCGAGGATTATCATGTGATAGAGCTTGTCGGTGAAGGATCTTTTGGGAGGGTTTACAAAGGAAGACGCAAGTATACCGGCCAGACGGTGGCGATGAAGTTCATCATGAAACAAGGAAAGACTGACAAGGATATACATAGTCTAAGGCAAGAAATCGAGATATTGCGGAAGCTCAAGCATGAGAATATCATTGAAATGCTCGACTCCTTTGAAAACGCACGAGAGTTCTGTGTCGTCACTGAGTTTGCCCAAGGTGAATTGTTTGAGATTCTTGAAGATGACAAGTGCCTTCCCGAAGAGCAAGTTCAAGCAATCGCAAAGCAATTGGTAAAAGCATTAGATTACTTGCATTCCAACCGTATAATTCACCGTGACATGAAGCCACAAAACATTCTTATTGGTGCTGGCTCTGTCGTTAAGCTATGTGATTTTGGTTTTGCTCGAGCTATGTCCACCAACACTGTGGTTTTGCGATCCATTAAAGGCACTCCGCTGTACATGGCACCAGAGCTTGTGAAAGAACAGCCATACGATCGCACTGTCGACCTGTGGTCTCTTGGTGTTATATTGTACGAGTTATATGTAGGTCAGCCTCCCTTCTACACCAATTCTGTGTATGCTCTCATTCGTCACATTGTTAAGGATCCTGTCAAATATCCTGATGAAATGAGTACTTATTTCGAAAGCTTTTTGAAAGGATTGCTCAACAAGGAACCCCACAGTCGATTAACCTGGCCTGCTCTTCGTGAGCACCCTTTCGTTAAAGAAACACAAGAAGAAGTTGAAGCCAGGGAAATCCATACTGCAGTAGTTGACAATAAGGCAGCTTGGATGCTCAAAGGAAATGGAGGGCAACAGAGAAATGAAAAGTGCGACTCAGTGACCCTGGTTGAGGACATGTCTGCTACAAAAGGTCTAGCTGACGTTCAGTCAGATATGAAGAGTGCTGTCAAAGTAAATTCTCCACCCACTGAGGATTTTGTGGGATTCCCAACCCAAGAGGAGATAAAAAGTTCAGGTAACCCAACACTGGACAAATTGGAAAATACATCCCGCACTGTTAAAGGTGCACAAGTTATAGGTGAAAATGATAAAGCATTGGACCTTGTTTTGCTGTCACTGGAAAGATTTTCAAAATCGCCAGATTCTAAAAGGGACAAAGATGTTGCTTGCTCTGTTCAGTCCCTCAGGATCATCTCCAATTTGGTTGCAACTCGTGCCATAGTTTCAGTTGGACTGATTGAGAAAATAACATGTGCACTGCTAGACTTTACTGACGCTCTTGTTGGGATGAAATCACCCGAGTTCAACAACATAATACCAAAGAGTCTTTCAGTCACTAAAAACTTGGTAGGGCATGTTGAAGGCAATAACATACATAGTTCTTACATCAGGCACTGGACCAAAGTAGTGGAAATCTTCATACAGGTCGTTAGATGGGAAGAAGAGGGAACTGGTAGAATTATATATGAGGCCTGTTCTTGCATCACAACAATGTTATCTCGAGTTGCCCAAGATCTTAAATCTTCAACTCCTGATTCTGTTTCTAAACAGATTTTGGAGCATGCTAACATGTCTCGCATAGTTGATCATTTGTGTCTTTGTCTGGCTTCTTCTGGGTCGAGTTTAACTTCCGGCTCTTCGCAAATGTTAGCAGCTGCCTGTGAAGCTTGTCGAGCAATATGGATTCTCATAGATACTTCCGAAACATTCTTCAAGAACGATGATGTAAACATATTACCTCTAGATGCCTTGCAGAACCGTCTCTCTCAGCATGACATAGGAAACAGTGAGTGGGGTCCGTTATCTGAGAAACTTGTCGATACAGTGACAAGAGCATATCTCCGATCAAAGCATGTGCAAGTTGCTGTTGGTCATTGCCTTCACCAACGAGTGGAGGCTCCATTGGTTTCTGCAATTCAGCTCTTGTCAAGATGCTGCCTTCATAATGGAATTCTGCCTAGCATGCTTTGTGGTCTTCCCAGTTCACTGCCTATTACGACCGTAGTCAGTGGTGGAGAGGATGGTACAGTTATTTCAGAAATATTTTCTATACTATCATACGCAACATTATCAAGCAAAGACCAGCAAACAGGAGAAAAAGATAACTTCGAGGGCAGACTAAACAATCTGGTGTTCCATTCATGCCTTATGTTGGCAACAGTTGCTCAATGTTTGAAGTTAACTGGGAGAAATTCTGTTCTGTTAATGCTTACAACTTCTCCAAAGAAGCATCAACATCGTCTTTCTGCTATAGCCAATCACATTGCCTCAGATGATAAAATCGAAGCTTCTCTTCAGAATCACTCTGCTTCAGCTATGCTTGCTCTCGCATCTATTCTCGCTCTTGAAAAAGGATCTTCTGCTGGATCTTCTGTCTCTGAGCTAGTGGTGTCTCTGATTCCTCGAGCTACTAAGCTTTGTTATCATCTTAGGCCTATGCCAAGTAATGAAGGTGAAGTCATCTCTCATTCTGCCAATTATGCCAAATGGCACGGACTTCTGGATGGATGTATCGGTTTATTAGAATCCAGATTGAAGTGGGGTGGACCTCTAGCTGTGCAGCAGCTTATTGCTAGTGGTACGCCATTGCTTCTCATAAATCTGTTAGCTGGCAAACTTTCAAATGCTTCCCCAGAGGACATCAAGAAGACATCCAACCGGATTGGCTTGTCCCCTATTGGCGTTGTATGGACTATTTCGTCCATATGCCACTGTCTTTCGGGTGGCACAACTTTCCGTCAGGTTCTTGTGAAAATCGAAACCATGAAGCTAATTACTTGTTTACTATCTGATGCTCATATCAAGCTAGTAAAGAGCTGGGGAGGTCCGGGGGGAGGAAAAGATGGGGTCAGAGAGACAATAAATGTGATAATTGATCTCCTAGCATTTCCTTTTGTTGCTCTGCAAAGTCAACCAGGTTCGTTATCTGCCACAGCTTCTGTAAATAGTGGATTCATTCTCAACATTGGCTCTCCTGGTGTGAGAGTATGCATGGAAGATAGAGATTTGCTAAAAGCTATAGAAGAGGATATGGACAAATACATAATAGTCCTCCTGGAGGTGGGAGTGCCGAGTTTAATCCTACGTTGCTTGGATCATTTGGAATTAAAAGATTTAGTAAGGCCCGTTGCTTTTCTTGCCAAAATGGTGGGTCGTCCACGTCTTGCTGTAGATCTCGTGAGCAAAGGTCTGTTAGATCCTAATAGAATGAAAAAATTACTCAACCAATCAAGTCCAAGAGAAGTCATACTTGATATTTTGATGATCATATCTGATCTATCGAGGATGGATAAGGCTTTTTATAAATACATCGGCGAGGCTTCTGTTTTACAGCCTTTGAAAGAATATCTTACCCATGTGGATCCAAATATACGTGCAAAAGCTTGTAGTGCTCTTGGCAATATGTGCAGACACAATGGATATTTCTACAGCGCTCTTGCGGAACATCAAATCATCGGTCTCCTCATTGATCGATGCGCTGATCCGGACAAACGAACACAGAAATTCGCTTGTTTTGCTATTGGAAATGCGGCTTACCATAACGACACGCTGTATGAAGAGCTAAGAAGATCTATAACGCAGCTAGCAAACGTTTTGACCACGGCTGAAGAAGACAAGACAAAAGCAAACGCGGCTGGTGCATTGAGCAACCTTGTCCGAAACTCCAACAAACTCTGTGAAGATATAGTCTCCAAGGGAGCTTTACAAACGTTACTGAGGCTAGTTGCGGATTGCTCGACTCTTGCCTTGAACCCAAGCAAGAAAGAGACAGCGAGTGAGTCACCGCTCAAGATTGCACTCTTCTCATTGGCTAAAATGTGTTCGAACCACCAGATTTGCAGACAGTTTGTGAAGTCATCAGAGTTGTTCCCGGTCATTGCAAGGCTTAAACAATCCCCTGAGGCTAACATTGCTCACTATGCTTCAGTTATTGTCGCCAAAGTCAGTGGTGAGTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000166 GO:0000278 GO:0000280 GO:0000281 GO:0000910 GO:0000911 GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005856 GO:0005874 GO:0005881 GO:0006355 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006935 GO:0006996 GO:0007049 GO:0007112 GO:0007140 GO:0007154 GO:0007163 GO:0007165 GO:0007166 GO:0007224 GO:0007227 GO:0007228 GO:0007275 GO:0007276 GO:0007349 GO:0007584 GO:0008064 GO:0008092 GO:0008134 GO:0008144 GO:0008150 GO:0008152 GO:0008589 GO:0009524 GO:0009553 GO:0009558 GO:0009561 GO:0009605 GO:0009653 GO:0009791 GO:0009889 GO:0009966 GO:0009967 GO:0009987 GO:0009991 GO:0010033 GO:0010243 GO:0010468 GO:0010556 GO:0010639 GO:0010646 GO:0010647 GO:0014070 GO:0015630 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017076 GO:0019219 GO:0019222 GO:0019538 GO:0019894 GO:0019953 GO:0022402 GO:0022412 GO:0022414 GO:0022607 GO:0023051 GO:0023052 GO:0023056 GO:0030010 GO:0030030 GO:0030031 GO:0030554 GO:0030832 GO:0030833 GO:0030837 GO:0031156 GO:0031272 GO:0031323 GO:0031326 GO:0031333 GO:0031344 GO:0031667 GO:0032101 GO:0032104 GO:0032107 GO:0032271 GO:0032272 GO:0032501 GO:0032502 GO:0032504 GO:0032506 GO:0032535 GO:0032553 GO:0032555 GO:0032559 GO:0032956 GO:0032970 GO:0033043 GO:0033206 GO:0033273 GO:0035556 GO:0035639 GO:0036094 GO:0036211 GO:0040011 GO:0042221 GO:0042330 GO:0042493 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043254 GO:0043326 GO:0043327 GO:0043412 GO:0043900 GO:0044085 GO:0044087 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0044703 GO:0044782 GO:0045880 GO:0048229 GO:0048232 GO:0048285 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048609 GO:0048646 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051090 GO:0051091 GO:0051128 GO:0051129 GO:0051171 GO:0051252 GO:0051301 GO:0051321 GO:0051493 GO:0051494 GO:0051593 GO:0051704 GO:0051716 GO:0060176 GO:0060255 GO:0060271 GO:0060491 GO:0061640 GO:0065007 GO:0065008 GO:0065009 GO:0070925 GO:0071704 GO:0071840 GO:0080090 GO:0080134 GO:0080135 GO:0090066 GO:0097159 GO:0097367 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0110053 GO:0120031 GO:0120032 GO:0120035 GO:0120036 GO:0140013 GO:0140096 GO:1901265 GO:1901363 GO:1901564 GO:1901698 GO:1901700 GO:1902410 GO:1902903 GO:1902904 GO:1903046 GO:1903047 GO:1903506 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04341 map04341
Pfam Domains
Protein Families
Arm HEAT HEAT_2 Pkinase

Protein Analysis

1322

Amino Acids

145.41

Weight (kDa)

7.19

Isoelectric Point (pI)

47.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 6 - 256 1.5e-77 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 8 - 251 1.4e-45 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3312
AasI GACNNNNNNGTC 1 cut(s) 2707
AccB1I GGYRCC 1 cut(s) 508
AccB7I CCANNNNNTGG 2 cut(s) 94, 1900
AccBSI CCGCTC 1 cut(s) 3796
AccI GTMKAC 2 cut(s) 80, 549
AccII CGCG 1 cut(s) 3641
AccIII TCCGGA 1 cut(s) 3493
AciI CCGC 8 cut(s) 172, 497, 1060, 3449, 3539, 3641, 3737, 3794
AclI AACGTT 2 cut(s) 3600, 3719
AcoI YGGCCR 1 cut(s) 85
AcsI RAATTY 4 cut(s) 964, 1959, 2200, 3512
AcuI CTGAAG 4 cut(s) 2288, 2304, 3636, 3918
AfaI GTAC 5 cut(s) 503, 578, 679, 2035, 2590
AfeI AGCGCT 1 cut(s) 3442
AfiI CCNNNNNNNGG 8 cut(s) 94, 898, 1036, 1900, 2161, 2843, 3067, 3070
AflIII ACRYGT 3 cut(s) 903, 1259, 1578
AhdI GACNNNNNGTC 2 cut(s) 556, 934
AjiI CACGTC 1 cut(s) 3170
AjnI CCWGG 6 cut(s) 737, 794, 891, 2923, 2983, 3063
AjuI GAANNNNNNNTTGG 4 cut(s) 33, 65, 1321, 1353
Alw21I GWGCWC 4 cut(s) 759, 1078, 1267, 3403
Alw26I GTCTC 9 cut(s) 564, 1587, 1775, 2389, 2406, 2858, 3473, 3703, 3770
Alw44I GTGCAC 2 cut(s) 1074, 1263
AlwNI CAGNNNCTG 2 cut(s) 1404, 2948
Ama87I CYCGRG 4 cut(s) 447, 1307, 1509, 2412
Aor13HI TCCGGA 1 cut(s) 3493
Aor51HI AGCGCT 1 cut(s) 3442
AoxI GGCC 5 cut(s) 85, 740, 1479, 2441, 3131
ApaLI GTGCAC 2 cut(s) 1074, 1263
ApeKI GCWGC 7 cut(s) 830, 1661, 1664, 1944, 2569, 2572, 3589
ApoI RAATTY 4 cut(s) 964, 1959, 2200, 3512
ArsI GACNNNNNNTTYG 2 cut(s) 3451, 3483
AspLEI GCGC 2 cut(s) 3443, 3488
AspS9I GGNCC 6 cut(s) 1108, 1407, 1805, 2555, 2834, 3132
AsuC2I CCSGG 2 cut(s) 2838, 3881
AsuHPI GGTGA 8 cut(s) 50, 272, 365, 1100, 1296, 1883, 2474, 3783
AsuII TTCGAA 2 cut(s) 687, 3833
AsuNHI GCTAGC 1 cut(s) 3592
AvaI CYCGRG 4 cut(s) 447, 1307, 1509, 2412
AvaII GGWCC 5 cut(s) 1108, 1407, 1805, 2555, 2834
AxyI CCTNAGG 2 cut(s) 1193, 3909
BaeGI GKGCMC 2 cut(s) 1078, 1267
BamHI GGATCC 2 cut(s) 649, 3370
BanI GGYRCC 1 cut(s) 508
BauI CACGAG 3 cut(s) 225, 1221, 3185
BbsI GAAGAC 4 cut(s) 76, 1976, 2663, 3627
Bbv12I GWGCWC 4 cut(s) 759, 1078, 1267, 3403
BbvI GCAGC 7 cut(s) 842, 1651, 1673, 1931, 2581, 2584, 3601
BccI CCATC 5 cut(s) 1437, 2024, 2507, 2846, 3289
BceAI ACGGC 1 cut(s) 3627
BcgI CGANNNNNNTGC 2 cut(s) 2228, 2262
BciT130I CCWGG 6 cut(s) 739, 796, 893, 2925, 2985, 3065
BclI TGATCA 2 cut(s) 1594, 3273
BcnI CCSGG 2 cut(s) 2838, 3881
BcoDI GTCTC 9 cut(s) 564, 1587, 1775, 2389, 2406, 2858, 3473, 3703, 3770
BfmI CTRYAG 5 cut(s) 810, 2259, 3027, 3177, 3436
BfoI RGCGCY 1 cut(s) 3444
BglII AGATCT 3 cut(s) 1522, 3181, 3578
BisI GCNGC 9 cut(s) 831, 1662, 1665, 1945, 2570, 2573, 3540, 3590, 3642
BlsI GCNGC 9 cut(s) 832, 1663, 1666, 1946, 2571, 2574, 3541, 3591, 3643
BmcAI AGTACT 1 cut(s) 679
Bme1390I CCNGG 8 cut(s) 739, 796, 893, 2838, 2925, 2985, 3065, 3881
Bme18I GGWCC 5 cut(s) 1108, 1407, 1805, 2555, 2834
BmeRI GACNNNNNGTC 2 cut(s) 556, 934
BmeT110I CYCGRG 4 cut(s) 447, 1307, 1509, 2412
BmgBI CACGTC 1 cut(s) 3170
BmgT120I GGNCC 6 cut(s) 1108, 1407, 1805, 2555, 2834, 3132
BmiI GGNNCC 6 cut(s) 510, 651, 720, 1806, 1908, 3372
BmrFI CCNGG 8 cut(s) 739, 796, 893, 2838, 2925, 2985, 3065, 3881
BmrI ACTGGG 2 cut(s) 1983, 2202
BmsI GCATC 8 cut(s) 828, 1500, 1744, 1931, 2248, 2348, 2791, 3473
BmtI GCTAGC 1 cut(s) 3596
BmuI ACTGGG 2 cut(s) 1983, 2202
BoxI GACNNNNGTC 1 cut(s) 2012
BpiI GAAGAC 4 cut(s) 76, 1976, 2663, 3627
BplI GAGNNNNNCTC 2 cut(s) 1833, 1865
BpmI CTGGAG 1 cut(s) 3086
Bpu14I TTCGAA 2 cut(s) 687, 3833
BpuEI CTTGAG 2 cut(s) 164, 3782
BpuMI CCSGG 2 cut(s) 2838, 3881
Bsa29I ATCGAT 1 cut(s) 3481
BsaAI YACGTR 1 cut(s) 3383
BsaI GGTCTC 2 cut(s) 564, 3473
BsaJI CCNNGG 6 cut(s) 256, 795, 891, 2837, 3609, 3702
BsaWI WCCGGW 2 cut(s) 2667, 3493
BsaXI ACNNNNNCTCC 2 cut(s) 2212, 2242
Bsc4I CCNNNNNNNGG 8 cut(s) 94, 898, 1036, 1900, 2161, 2843, 3067, 3070
Bse118I RCCGGY 1 cut(s) 83
Bse1I ACTGG 6 cut(s) 1041, 1131, 1409, 1465, 1989, 2197
Bse21I CCTNAGG 2 cut(s) 1193, 3909
Bse3DI GCAATG 5 cut(s) 1882, 2271, 2594, 3885, 3918
BseAI TCCGGA 1 cut(s) 3493
BseBI CCWGG 6 cut(s) 739, 796, 893, 2925, 2985, 3065
BseCI ATCGAT 1 cut(s) 3481
BseDI CCNNGG 6 cut(s) 256, 795, 891, 2837, 3609, 3702
BseGI GGATG 8 cut(s) 843, 1055, 1302, 2035, 2518, 2522, 2660, 3300
BseLI CCNNNNNNNGG 8 cut(s) 94, 898, 1036, 1900, 2161, 2843, 3067, 3070
BseMI GCAATG 5 cut(s) 1882, 2271, 2594, 3885, 3918
BseNI ACTGG 6 cut(s) 1041, 1131, 1409, 1465, 1989, 2197
BseRI GAGGAG 2 cut(s) 1022, 3461
BseSI GKGCMC 2 cut(s) 1078, 1267
BseXI GCAGC 7 cut(s) 842, 1651, 1673, 1931, 2581, 2584, 3601
BseYI CCCAGC 1 cut(s) 2826
BsgI GTGCAG 2 cut(s) 2588, 3436
Bsh1236I CGCG 1 cut(s) 3641
Bsh1285I CGRYCG 2 cut(s) 541, 2010
BshFI GGCC 5 cut(s) 87, 742, 1481, 2443, 3133
BshNI GGYRCC 1 cut(s) 508
BshVI ATCGAT 1 cut(s) 3481
BsiEI CGRYCG 2 cut(s) 541, 2010
BsiHKAI GWGCWC 4 cut(s) 759, 1078, 1267, 3403
BsiHKCI CYCGRG 4 cut(s) 447, 1307, 1509, 2412
BsiSI CCGG 6 cut(s) 84, 1641, 2668, 2837, 3494, 3881
BslFI GGGAC 3 cut(s) 1174, 1175, 2665
BslI CCNNNNNNNGG 8 cut(s) 94, 898, 1036, 1900, 2161, 2843, 3067, 3070
BsmAI GTCTC 9 cut(s) 564, 1587, 1775, 2389, 2406, 2858, 3473, 3703, 3770
BsmBI CGTCTC 1 cut(s) 1775
BsmFI GGGAC 3 cut(s) 1174, 1175, 2665
BsmI GAATGC 1 cut(s) 355
BsnI GGCC 5 cut(s) 87, 742, 1481, 2443, 3133
Bso31I GGTCTC 2 cut(s) 564, 3473
BsoBI CYCGRG 4 cut(s) 447, 1307, 1509, 2412
Bsp119I TTCGAA 2 cut(s) 687, 3833
Bsp1286I GDGCHC 4 cut(s) 759, 1078, 1267, 3403
Bsp13I TCCGGA 1 cut(s) 3493
Bsp1407I TGTACA 1 cut(s) 501
BspACI CCGC 8 cut(s) 172, 497, 1060, 3449, 3539, 3641, 3737, 3794
BspANI GGCC 5 cut(s) 87, 742, 1481, 2443, 3133
BspDI ATCGAT 1 cut(s) 3481
BspEI TCCGGA 1 cut(s) 3493
BspFNI CGCG 1 cut(s) 3641
BspHI TCATGA 1 cut(s) 111
BspLI GGNNCC 6 cut(s) 510, 651, 720, 1806, 1908, 3372
BspMAI CTGCAG 1 cut(s) 814
BspOI GCTAGC 1 cut(s) 3596
BspQI GCTCTTC 4 cut(s) 297, 753, 1651, 3561
BspT104I TTCGAA 2 cut(s) 687, 3833
BspT107I GGYRCC 1 cut(s) 508
BspTNI GGTCTC 2 cut(s) 564, 3473
BsrBI CCGCTC 1 cut(s) 3796
BsrDI GCAATG 5 cut(s) 1882, 2271, 2594, 3885, 3918
BsrFI RCCGGY 1 cut(s) 83
BsrGI TGTACA 1 cut(s) 501
BsrI ACTGG 6 cut(s) 1041, 1131, 1409, 1465, 1989, 2197
BssAI RCCGGY 1 cut(s) 83
BssECI CCNNGG 6 cut(s) 256, 795, 891, 2837, 3609, 3702
BssNAI GTATAC 1 cut(s) 81
BssSI CACGAG 3 cut(s) 225, 1221, 3185
BssT1I CCWWGG 2 cut(s) 256, 3702
Bst1107I GTATAC 1 cut(s) 81
Bst2BI CACGAG 3 cut(s) 225, 1221, 3185
Bst2UI CCWGG 6 cut(s) 739, 796, 893, 2925, 2985, 3065
Bst6I CTCTTC 9 cut(s) 297, 753, 941, 1446, 1651, 2307, 3027, 3561, 3815
BstAPI GCANNNNNTGC 3 cut(s) 1223, 2171, 3414
BstAUI TGTACA 1 cut(s) 501
BstBAI YACGTR 1 cut(s) 3383
BstBI TTCGAA 2 cut(s) 687, 3833
BstC8I GCNNGC 8 cut(s) 412, 744, 1177, 1572, 1973, 2331, 2620, 3594
BstDSI CCRYGG 1 cut(s) 3609
BstEII GGTNACC 1 cut(s) 1025
BstENI CCTNNNNNAGG 2 cut(s) 896, 3065
BstF5I GGATG 8 cut(s) 843, 1055, 1302, 2035, 2518, 2522, 2660, 3300
BstFNI CGCG 1 cut(s) 3641
BstH2I RGCGCY 1 cut(s) 3444
BstHHI GCGC 2 cut(s) 3443, 3488
BstMAI GTCTC 9 cut(s) 564, 1587, 1775, 2389, 2406, 2858, 3473, 3703, 3770
BstMCI CGRYCG 2 cut(s) 541, 2010
BstNI CCWGG 6 cut(s) 739, 796, 893, 2925, 2985, 3065
BstNSI RCATGY 7 cut(s) 907, 1263, 1367, 1574, 1582, 1865, 1975
BstPAI GACNNNNGTC 1 cut(s) 2012
BstPI GGTNACC 1 cut(s) 1025
BstSCI CCNGG 8 cut(s) 737, 794, 891, 2836, 2923, 2983, 3063, 3879
BstSFI CTRYAG 5 cut(s) 810, 2259, 3027, 3177, 3436
BstSLI GKGCMC 2 cut(s) 1078, 1267
BstUI CGCG 1 cut(s) 3641
BstV1I GCAGC 7 cut(s) 842, 1651, 1673, 1931, 2581, 2584, 3601
BstV2I GAAGAC 4 cut(s) 76, 1976, 2663, 3627
BstX2I RGATCY 9 cut(s) 44, 649, 1522, 2363, 2375, 3181, 3205, 3370, 3578
BstXI CCANNNNNNTGG 3 cut(s) 469, 1417, 3157
BstYI RGATCY 9 cut(s) 44, 649, 1522, 2363, 2375, 3181, 3205, 3370, 3578
BstZ17I GTATAC 1 cut(s) 81
Bsu15I ATCGAT 1 cut(s) 3481
Bsu36I CCTNAGG 2 cut(s) 1193, 3909
BsuRI GGCC 5 cut(s) 87, 742, 1481, 2443, 3133
BsuTUI ATCGAT 1 cut(s) 3481
BtgI CCRYGG 1 cut(s) 3609
BtgZI GCGATG 1 cut(s) 113
BtrI CACGTC 1 cut(s) 3170
BtsCI GGATG 8 cut(s) 843, 1055, 1302, 2035, 2518, 2522, 2660, 3300
BtsI GCAGTG 2 cut(s) 1265, 1994
Cac8I GCNNGC 8 cut(s) 412, 744, 1177, 1572, 1973, 2331, 2620, 3594
CaiI CAGNNNCTG 2 cut(s) 1404, 2948
CciI TCATGA 1 cut(s) 111
CfoI GCGC 2 cut(s) 3443, 3488
Cfr10I RCCGGY 1 cut(s) 83
Cfr13I GGNCC 6 cut(s) 1108, 1407, 1805, 2555, 2834, 3132
ClaI ATCGAT 1 cut(s) 3481
CseI GACGC 2 cut(s) 81, 1292
CsiI ACCWGGT 1 cut(s) 2923
Csp6I GTAC 5 cut(s) 502, 577, 678, 2034, 2589
CspCI CAANNNNNGTGG 3 cut(s) 3827, 3862, 3937
CviQI GTAC 5 cut(s) 502, 577, 678, 2034, 2589
DrdI GACNNNNNNGTC 1 cut(s) 2707
DriI GACNNNNNGTC 2 cut(s) 556, 934
DseDI GACNNNNNNGTC 1 cut(s) 2707
EaeI YGGCCR 1 cut(s) 85
Eam1104I CTCTTC 9 cut(s) 297, 753, 941, 1446, 1651, 2307, 3027, 3561, 3815
Eam1105I GACNNNNNGTC 2 cut(s) 556, 934
EarI CTCTTC 9 cut(s) 297, 753, 941, 1446, 1651, 2307, 3027, 3561, 3815
Eco130I CCWWGG 2 cut(s) 256, 3702
Eco147I AGGCCT 2 cut(s) 1481, 2443
Eco31I GGTCTC 2 cut(s) 564, 3473
Eco47I GGWCC 5 cut(s) 1108, 1407, 1805, 2555, 2834
Eco47III AGCGCT 1 cut(s) 3442
Eco57I CTGAAG 4 cut(s) 2288, 2304, 3636, 3918
Eco81I CCTNAGG 2 cut(s) 1193, 3909
Eco88I CYCGRG 4 cut(s) 447, 1307, 1509, 2412
Eco91I GGTNACC 1 cut(s) 1025
EcoNI CCTNNNNNAGG 2 cut(s) 896, 3065
EcoO65I GGTNACC 1 cut(s) 1025
EcoRI GAATTC 1 cut(s) 1959
EcoRII CCWGG 6 cut(s) 737, 794, 891, 2923, 2983, 3063
EcoT14I CCWWGG 2 cut(s) 256, 3702
EcoT22I ATGCAT 1 cut(s) 3002
ErhI CCWWGG 2 cut(s) 256, 3702
Esp3I CGTCTC 1 cut(s) 1775
FalI AAGNNNNNCTT 6 cut(s) 2286, 2318, 3243, 3275, 3342, 3374
FaqI GGGAC 3 cut(s) 1174, 1175, 2665
FauI CCCGC 1 cut(s) 1067
FauNDI CATATG 1 cut(s) 2714
FbaI TGATCA 2 cut(s) 1594, 3273
FblI GTMKAC 2 cut(s) 80, 549
Fnu4HI GCNGC 9 cut(s) 831, 1662, 1665, 1945, 2570, 2573, 3540, 3590, 3642
FokI GGATG 8 cut(s) 850, 1042, 1309, 2042, 2525, 2529, 2647, 3307
Fsp4HI GCNGC 9 cut(s) 831, 1662, 1665, 1945, 2570, 2573, 3540, 3590, 3642
GlaI GCGC 2 cut(s) 3442, 3487
GluI GCNGC 9 cut(s) 831, 1662, 1665, 1945, 2570, 2573, 3540, 3590, 3642
GsaI CCCAGC 1 cut(s) 2830
GsuI CTGGAG 1 cut(s) 3086
HaeII RGCGCY 1 cut(s) 3444
HaeIII GGCC 5 cut(s) 87, 742, 1481, 2443, 3133
HapII CCGG 6 cut(s) 84, 1641, 2668, 2837, 3494, 3881
HgaI GACGC 2 cut(s) 81, 1292
HhaI GCGC 2 cut(s) 3443, 3488
Hin6I GCGC 2 cut(s) 3441, 3486
HinP1I GCGC 2 cut(s) 3441, 3486
HincII GTYRAC 4 cut(s) 550, 820, 2190, 2921
HindII GTYRAC 4 cut(s) 550, 820, 2190, 2921
HindIII AAGCTT 5 cut(s) 691, 1674, 2295, 2423, 3390
HpaI GTTAAC 1 cut(s) 2190
HpaII CCGG 6 cut(s) 84, 1641, 2668, 2837, 3494, 3881
HphI GGTGA 8 cut(s) 50, 272, 365, 1100, 1296, 1883, 2474, 3783
HpyAV CCTTC 7 cut(s) 35, 305, 613, 1364, 1898, 1958, 2453
HpyCH4IV ACGT 6 cut(s) 930, 3094, 3169, 3382, 3600, 3719
HpySE526I ACGT 6 cut(s) 930, 3094, 3169, 3382, 3600, 3719
HspAI GCGC 2 cut(s) 3441, 3486
Kpn2I TCCGGA 1 cut(s) 3493
Ksp22I TGATCA 2 cut(s) 1594, 3273
KspAI GTTAAC 1 cut(s) 2190
LguI GCTCTTC 4 cut(s) 297, 753, 1651, 3561
LmnI GCTCC 3 cut(s) 1567, 1912, 3707
Lsp1109I GCAGC 7 cut(s) 842, 1651, 1673, 1931, 2581, 2584, 3601
LweI GCATC 8 cut(s) 828, 1500, 1744, 1931, 2248, 2348, 2791, 3473
MabI ACCWGGT 1 cut(s) 2923
MaeII ACGT 6 cut(s) 930, 3094, 3169, 3382, 3600, 3719
MbiI CCGCTC 1 cut(s) 3796
MfeI CAATTG 1 cut(s) 329
MflI RGATCY 9 cut(s) 44, 649, 1522, 2363, 2375, 3181, 3205, 3370, 3578
MhlI GDGCHC 4 cut(s) 759, 1078, 1267, 3403
MlyI GAGTC 5 cut(s) 203, 875, 1342, 3742, 3797
MmeI TCCRAC 4 cut(s) 384, 1219, 2688, 3699
Mph1103I ATGCAT 1 cut(s) 3002
MroI TCCGGA 1 cut(s) 3493
MslI CAYNNNNRTG 2 cut(s) 200, 1499
MspA1I CMGCKG 2 cut(s) 499, 1664
MspI CCGG 6 cut(s) 84, 1641, 2668, 2837, 3494, 3881
MspR9I CCNGG 8 cut(s) 739, 796, 893, 2838, 2925, 2985, 3065, 3881
MunI CAATTG 1 cut(s) 329
Mva1269I GAATGC 1 cut(s) 355
MvaI CCWGG 6 cut(s) 739, 796, 893, 2925, 2985, 3065
MvnI CGCG 1 cut(s) 3641
NciI CCSGG 2 cut(s) 2838, 3881
NdeI CATATG 1 cut(s) 2714
NheI GCTAGC 1 cut(s) 3592
NlaIV GGNNCC 6 cut(s) 510, 651, 720, 1806, 1908, 3372
NmeAIII GCCGAG 1 cut(s) 3105
NmuCI GTSAC 8 cut(s) 241, 377, 635, 886, 1122, 1342, 1834, 3789
NsiI ATGCAT 1 cut(s) 3002
NspI RCATGY 7 cut(s) 907, 1263, 1367, 1574, 1582, 1865, 1975
NspV TTCGAA 2 cut(s) 687, 3833
PaeI GCATGC 2 cut(s) 1574, 1975
PaeR7I CTCGAG 3 cut(s) 447, 1509, 2412
PagI TCATGA 1 cut(s) 111
PceI AGGCCT 2 cut(s) 1481, 2443
PciI ACATGT 3 cut(s) 903, 1259, 1578
PciSI GCTCTTC 4 cut(s) 297, 753, 1651, 3561
PctI GAATGC 1 cut(s) 355
PfeI GAWTC 9 cut(s) 274, 993, 1151, 1544, 1693, 2308, 2407, 2534, 2961
PflMI CCANNNNNTGG 2 cut(s) 94, 1900
PfoI TCCNGGA 1 cut(s) 3063
PkrI GCNGC 9 cut(s) 832, 1663, 1666, 1946, 2571, 2574, 3541, 3591, 3643
Ple19I CGATCG 1 cut(s) 541
PleI GAGTC 5 cut(s) 203, 875, 1341, 3742, 3796
PpsI GAGTC 5 cut(s) 203, 875, 1341, 3742, 3796
Ppu21I YACGTR 1 cut(s) 3383
PscI ACATGT 3 cut(s) 903, 1259, 1578
PshAI GACNNNNGTC 1 cut(s) 2012
PsiI TTATAA 1 cut(s) 3312
Psp1406I AACGTT 2 cut(s) 3600, 3719
Psp6I CCWGG 6 cut(s) 737, 794, 891, 2923, 2983, 3063
PspEI GGTNACC 1 cut(s) 1025
PspFI CCCAGC 1 cut(s) 2826
PspGI CCWGG 6 cut(s) 737, 794, 891, 2923, 2983, 3063
PspN4I GGNNCC 6 cut(s) 510, 651, 720, 1806, 1908, 3372
PspPI GGNCC 6 cut(s) 1108, 1407, 1805, 2555, 2834, 3132
PspXI VCTCGAGB 2 cut(s) 447, 2412
PstI CTGCAG 1 cut(s) 814
PstNI CAGNNNCTG 2 cut(s) 1404, 2948
PsuI RGATCY 9 cut(s) 44, 649, 1522, 2363, 2375, 3181, 3205, 3370, 3578
PvuI CGATCG 1 cut(s) 541
PvuII CAGCTG 1 cut(s) 1664
RsaI GTAC 5 cut(s) 503, 578, 679, 2035, 2590
RsaNI GTAC 5 cut(s) 502, 577, 678, 2034, 2589
RseI CAYNNNNRTG 2 cut(s) 200, 1499
SalI GTCGAC 1 cut(s) 548
SapI GCTCTTC 4 cut(s) 297, 753, 1651, 3561
SatI GCNGC 9 cut(s) 831, 1662, 1665, 1945, 2570, 2573, 3540, 3590, 3642
Sau96I GGNCC 6 cut(s) 1108, 1407, 1805, 2555, 2834, 3132
ScaI AGTACT 1 cut(s) 679
SchI GAGTC 5 cut(s) 203, 875, 1342, 3742, 3797
ScrFI CCNGG 8 cut(s) 739, 796, 893, 2838, 2925, 2985, 3065, 3881
SduI GDGCHC 4 cut(s) 759, 1078, 1267, 3403
SexAI ACCWGGT 1 cut(s) 2923
SfaNI GCATC 8 cut(s) 828, 1500, 1744, 1931, 2248, 2348, 2791, 3473
SfcI CTRYAG 5 cut(s) 810, 2259, 3027, 3177, 3436
Sfr274I CTCGAG 3 cut(s) 447, 1509, 2412
SfuI TTCGAA 2 cut(s) 687, 3833
SinI GGWCC 5 cut(s) 1108, 1407, 1805, 2555, 2834
SlaI CTCGAG 3 cut(s) 447, 1509, 2412
SmiMI CAYNNNNRTG 2 cut(s) 200, 1499
SmlI CTYRAG 5 cut(s) 179, 447, 1509, 2412, 3797
SmoI CTYRAG 5 cut(s) 179, 447, 1509, 2412, 3797
SphI GCATGC 2 cut(s) 1574, 1975
SseBI AGGCCT 2 cut(s) 1481, 2443
SsiI CCGC 8 cut(s) 172, 497, 1060, 3449, 3539, 3641, 3737, 3794
SspI AATATT 1 cut(s) 2052
StuI AGGCCT 2 cut(s) 1481, 2443
StyD4I CCNGG 8 cut(s) 737, 794, 891, 2836, 2923, 2983, 3063, 3879
StyI CCWWGG 2 cut(s) 256, 3702
TaiI ACGT 6 cut(s) 933, 3097, 3172, 3385, 3603, 3722
TaqII GACCGA 1 cut(s) 3455
TatI WGTACW 2 cut(s) 501, 677
TauI GCSGC 2 cut(s) 3542, 3644
TfiI GAWTC 9 cut(s) 274, 993, 1151, 1544, 1693, 2308, 2407, 2534, 2961
TseFI GTSAC 8 cut(s) 241, 377, 635, 886, 1122, 1342, 1834, 3789
TseI GCWGC 7 cut(s) 830, 1661, 1664, 1944, 2569, 2572, 3589
Tsp45I GTSAC 8 cut(s) 241, 377, 635, 886, 1122, 1342, 1834, 3789
TspGWI ACGGA 3 cut(s) 1797, 2517, 2735
Van91I CCANNNNNTGG 2 cut(s) 94, 1900
VneI GTGCAC 2 cut(s) 1074, 1263
VpaK11BI GGWCC 5 cut(s) 1108, 1407, 1805, 2555, 2834
XagI CCTNNNNNAGG 2 cut(s) 896, 3065
XapI RAATTY 4 cut(s) 964, 1959, 2200, 3512
XbaI TCTAGA 1 cut(s) 1750
XceI RCATGY 7 cut(s) 907, 1263, 1367, 1574, 1582, 1865, 1975
XcmI CCANNNNNNNNNTGG 1 cut(s) 466
XhoI CTCGAG 3 cut(s) 447, 1509, 2412
XmiI GTMKAC 2 cut(s) 80, 549
ZrmI AGTACT 1 cut(s) 679
Zsp2I ATGCAT 1 cut(s) 3002
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.