pycom01g24230

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0004502
Physical Location & Seq
Forward (+)
21206120 .. 21210856
4737 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g24230.17

Sequence Viewer

Length: 2202 bp
ATGGGTGTCGAGAACTACCACGTGATTGAGCTTGTAGGCGAAGGTTCCTTTGGGAAGGTATACAAGGGAAGGCGAAAGCACACGGGTCAGACTGTTGCGATGAAATTCATTATGAAGCATGGCAAAAGTGACAAAGATATCCACAATTTAAGACAAGAAATTGAGATCCTAAGAAAGTTGAAGCACGAAAACATCATTGAAATGCTTGATTCATTTGAAAGCCCACAAGAGTTCTGTGTTGTCACAGAATTTGCACAAGGTGAACTATTTGAAATTCTTGAGGATGATAAGTGCCTTCCTGAAGAACAAGTTCAAGCTATTGCAAAGCAGCTGGTGAGAGCATTACATTATCTGCATTCCAACCGTATCATCCATCGTGACATGAAACCACAAAACATTCTCATTGGTGCTGGTTCTATTGTTAAGCTCTGTGATTTTGGTTTTGCACGTGCAATGTCCACAAACACTGTTGTTTTGCGATCCATTAAAGGTACTCCTCTGTACATGGCGCCTGAACTAGTACGGGAACAACCCTACAACCACACTGCAGATCTGTGGTCTCTAGGAGTAATATTATATGAATTATTTGTAGGCCAGCCTCCATTTTATACAAACTCTGTATATGCACTCATCAGGCACATTGTTAAGGATCCAGTTAAATATCCTGACAACATAAGTCCAAGTTTTAAAAACTTTCTTAAGGGATTGCTTAATAAGGTGCCTCAAAATCGATTGACTTGGCCAGCTCTTCTGGAACACCCATTTGTCAAAGAGACGCCTCCCGAACTGGAGTCTAGGGAAATGCGTTCTGCAACTACTGCAGAAAGGGGATGTGTTGTGGCATGGAGGGGTGAAGGAAACAAAGTTCAAACATCAGATGGTCTGGCTGTTTCATCTCCTGTCCACTCTCCAGCTTCTTCAGAGAACAACAGTGGGATATGTTTTCAGAATAATGCTCAAGCAAATACTCCTGAATCCACAACAGTCAATTCTTCCCCAAATGAGTTCCCAGGATTCACAAATCCTAGTGAAGTCAAACAGTCAGGTTGCCAAATTTTGGACAGACTGGAAAATAACTCTCGTACAGTCAAGGGTGCACAAATAATAAGTCAGGATAATGAAGCATTAGCACATGTTTTGTTACCACTAAAAAGATGCTCCAATGGACCCCCAGATTCATGCAGGGATGAAGATATTCTTAATTCAAACCAGTCTTTTAGAATTCTTTCAAACTTAGTTGCAGCTGGTGCCTTCCATTCCAGCGAGCTGCTTGATGAAATAATACATGAACTTCTTGCTTACTCTGCCATCATTGTGAGCATGAAATCTTCTGAAGTAAATGAGTTAAAAGCGAAGAGTTTCTCAATAATTAAAATTTTGGTTGACAATGCTGGAAGCAGCATTGGTGGCTCATATTTCAGGCACTGGGTTGTCTTATCAGATATTTTTTCACAGGTTGTTGGTTGTAGTGAAGATGCATCTGGAAGAGTTTTGTATGAGTCCATTACTTGCATTGCTGTCATGCTAAGAAGAGTCACACAGGGCCTTAAAGCTTTTTCCTCAACTTCAGTTCCTGAGGCAGCTTCTGATCCTAATGAAGCACTGAAACAGATTCTGAATCACACTAAAACATCTGGTTTAGTGGACCAACTTTGTCTCTGCTTAGTAACCGCAGGGTCAAGTCTTATTTCAGGCTCTTCAAATATGCTGCGTGCGGCTTGTGAGGCATGTACGGCAATTTGGTTTCTGATAGATGCATCAGAAAATCTTTCTATGAAAAGAAATGCCTATTTATTCCCCCTAAACACTTTGCGCCGGCCTTCACCCCAACTTGACATTAGGGATCAAGATCAAGATTCTTTGATTGGGACAGAAGCAGCAAAACTTGTTGCTGTAATTACGAGAGCATTCCTTAGATCAAAAGCAGTACAAGTTGCTATTCATTATTGCCTTCATCAACGACTTGAAGCTTCGCTATATGCTGGCGTTCAGTTATTGTTGAGGTGCTGCTTGCATAATGGAATTGTTCCAGGTGTTCTCTGTGGCCTGCCTAGTTCCCTACCTGTAACTACGGTTGTTAGTGGTGGAGGTGATGGAACCATTATTTCAGAGATATTCTCTTTGTTGTCTTTGTGCATTTCATCTCAATTTCTAATAACGATCCCCAAGCAGTTGAGACAGCCACCTTCAAGAACAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000166 GO:0000278 GO:0000280 GO:0000281 GO:0000910 GO:0000911 GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005856 GO:0005874 GO:0005881 GO:0006355 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006935 GO:0006996 GO:0007049 GO:0007112 GO:0007140 GO:0007154 GO:0007163 GO:0007165 GO:0007166 GO:0007224 GO:0007227 GO:0007228 GO:0007275 GO:0007276 GO:0007349 GO:0007584 GO:0008064 GO:0008092 GO:0008134 GO:0008144 GO:0008150 GO:0008152 GO:0008589 GO:0009524 GO:0009553 GO:0009558 GO:0009561 GO:0009605 GO:0009653 GO:0009791 GO:0009889 GO:0009966 GO:0009967 GO:0009987 GO:0009991 GO:0010033 GO:0010243 GO:0010468 GO:0010556 GO:0010639 GO:0010646 GO:0010647 GO:0014070 GO:0015630 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017076 GO:0019219 GO:0019222 GO:0019538 GO:0019894 GO:0019953 GO:0022402 GO:0022412 GO:0022414 GO:0022607 GO:0023051 GO:0023052 GO:0023056 GO:0030010 GO:0030030 GO:0030031 GO:0030554 GO:0030832 GO:0030833 GO:0030837 GO:0031156 GO:0031272 GO:0031323 GO:0031326 GO:0031333 GO:0031344 GO:0031667 GO:0032101 GO:0032104 GO:0032107 GO:0032271 GO:0032272 GO:0032501 GO:0032502 GO:0032504 GO:0032506 GO:0032535 GO:0032553 GO:0032555 GO:0032559 GO:0032956 GO:0032970 GO:0033043 GO:0033206 GO:0033273 GO:0035556 GO:0035639 GO:0036094 GO:0036211 GO:0040011 GO:0042221 GO:0042330 GO:0042493 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043254 GO:0043326 GO:0043327 GO:0043412 GO:0043900 GO:0044085 GO:0044087 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0044703 GO:0044782 GO:0045880 GO:0048229 GO:0048232 GO:0048285 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048609 GO:0048646 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051090 GO:0051091 GO:0051128 GO:0051129 GO:0051171 GO:0051252 GO:0051301 GO:0051321 GO:0051493 GO:0051494 GO:0051593 GO:0051704 GO:0051716 GO:0060176 GO:0060255 GO:0060271 GO:0060491 GO:0061640 GO:0065007 GO:0065008 GO:0065009 GO:0070925 GO:0071704 GO:0071840 GO:0080090 GO:0080134 GO:0080135 GO:0090066 GO:0097159 GO:0097367 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0110053 GO:0120031 GO:0120032 GO:0120035 GO:0120036 GO:0140013 GO:0140096 GO:1901265 GO:1901363 GO:1901564 GO:1901698 GO:1901700 GO:1902410 GO:1902903 GO:1902904 GO:1903046 GO:1903047 GO:1903506 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04341 map04341
Pfam Domains
Protein Families
Arm HEAT HEAT_2 Pkinase

Protein Analysis

734

Amino Acids

80.71

Weight (kDa)

6.71

Isoelectric Point (pI)

53.27

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 508, 718, 1247
AccB7I CCANNNNNTGG 1 cut(s) 1057
AccI GTMKAC 1 cut(s) 60
AciI CCGC 2 cut(s) 1671, 1715
AclWI GGATC 7 cut(s) 160, 474, 644, 657, 1583, 1851, 2155
AcoI YGGCCR 1 cut(s) 740
AcsI RAATTY 6 cut(s) 104, 248, 273, 1053, 1221, 1374
AcuI CTGAAG 4 cut(s) 321, 903, 1353, 1551
AcvI CACGTG 2 cut(s) 22, 449
AcyI GRCGYC 2 cut(s) 509, 776
AdeI CACNNNGTG 1 cut(s) 260
AfaI GTAC 6 cut(s) 493, 503, 522, 1084, 1732, 1929
AfiI CCNNNNNNNGG 1 cut(s) 1057
AflII CTTAAG 1 cut(s) 698
AflIII ACRYGT 1 cut(s) 1132
AhlI ACTAGT 1 cut(s) 517
AjnI CCWGG 2 cut(s) 1009, 2029
AjuI GAANNNNNNNTTGG 2 cut(s) 33, 65
AloI GAACNNNNNNTCC 2 cut(s) 849, 881
Alw21I GWGCWC 1 cut(s) 1099
Alw26I GTCTC 4 cut(s) 564, 767, 1661, 2170
Alw44I GTGCAC 1 cut(s) 1095
AlwI GGATC 7 cut(s) 160, 474, 644, 657, 1583, 1851, 2155
AlwNI CAGNNNCTG 4 cut(s) 1425, 1574, 1586, 1615
AoxI GGCC 5 cut(s) 592, 740, 1543, 1817, 2044
ApaLI GTGCAC 1 cut(s) 1095
ApeKI GCWGC 8 cut(s) 328, 1241, 1267, 1398, 1580, 1708, 1877, 2007
ApoI RAATTY 6 cut(s) 104, 248, 273, 1053, 1221, 1374
ArsI GACNNNNNNTTYG 2 cut(s) 1093, 1125
Asp700I GAANNNNTTC 4 cut(s) 309, 1194, 1225, 1358
AspLEI GCGC 2 cut(s) 511, 1815
AspS9I GGNCC 3 cut(s) 1166, 1543, 1645
AsuHPI GGTGA 5 cut(s) 272, 346, 863, 1815, 2102
AvaII GGWCC 2 cut(s) 1166, 1645
AxyI CCTNAGG 1 cut(s) 1575
BaeGI GKGCMC 1 cut(s) 1099
BalI TGGCCA 1 cut(s) 742
BamHI GGATCC 1 cut(s) 649
BanI GGYRCC 3 cut(s) 508, 718, 1247
BbrPI CACGTG 2 cut(s) 22, 449
Bbv12I GWGCWC 1 cut(s) 1099
BbvI GCAGC 8 cut(s) 340, 1253, 1254, 1410, 1592, 1695, 1889, 1994
BccI CCATC 4 cut(s) 381, 872, 1316, 2087
BceAI ACGGC 1 cut(s) 1749
BciT130I CCWGG 2 cut(s) 1011, 2031
BcoDI GTCTC 4 cut(s) 564, 767, 1661, 2170
BcuI ACTAGT 1 cut(s) 517
BfaI CTAG 5 cut(s) 518, 563, 795, 1026, 2052
BfmI CTRYAG 2 cut(s) 546, 819
BfoI RGCGCY 1 cut(s) 512
BfrI CTTAAG 1 cut(s) 698
BglII AGATCT 1 cut(s) 550
BisI GCNGC 9 cut(s) 329, 1242, 1268, 1399, 1581, 1709, 1716, 1878, 2008
BlsI GCNGC 9 cut(s) 330, 1243, 1269, 1400, 1582, 1710, 1717, 1879, 2009
Bme1390I CCNGG 2 cut(s) 1011, 2031
Bme18I GGWCC 2 cut(s) 1166, 1645
BmgT120I GGNCC 3 cut(s) 1166, 1543, 1645
BmiI GGNNCC 7 cut(s) 46, 510, 651, 720, 1168, 1249, 2098
BmrFI CCNGG 2 cut(s) 1011, 2031
BmrI ACTGGG 1 cut(s) 1435
BmsI GCATC 5 cut(s) 1145, 1465, 1487, 1744, 1766
BmuI ACTGGG 1 cut(s) 1435
BplI GAGNNNNNCTC 2 cut(s) 2102, 2134
BpmI CTGGAG 2 cut(s) 809, 894
BpuEI CTTGAG 2 cut(s) 299, 942
Bsa29I ATCGAT 1 cut(s) 730
BsaAI YACGTR 2 cut(s) 22, 449
BsaBI GATNNNNATC 1 cut(s) 1848
BsaHI GRCGYC 2 cut(s) 509, 776
BsaI GGTCTC 1 cut(s) 564
BsaJI CCNNGG 1 cut(s) 1009
Bsc4I CCNNNNNNNGG 1 cut(s) 1057
Bse118I RCCGGY 1 cut(s) 1815
Bse1I ACTGG 5 cut(s) 653, 792, 1071, 1210, 1430
Bse21I CCTNAGG 1 cut(s) 1575
Bse3DI GCAATG 2 cut(s) 459, 1512
Bse8I GATNNNNATC 1 cut(s) 1848
BseBI CCWGG 2 cut(s) 1011, 2031
BseCI ATCGAT 1 cut(s) 730
BseDI CCNNGG 1 cut(s) 1009
BseGI GGATG 4 cut(s) 289, 369, 836, 1192
BseJI GATNNNNATC 1 cut(s) 1848
BseLI CCNNNNNNNGG 1 cut(s) 1057
BseMI GCAATG 2 cut(s) 459, 1512
BseMII CTCAG 1 cut(s) 1566
BseNI ACTGG 5 cut(s) 653, 792, 1071, 1210, 1430
BseRI GAGGAG 1 cut(s) 486
BseSI GKGCMC 1 cut(s) 1099
BseXI GCAGC 8 cut(s) 340, 1253, 1254, 1410, 1592, 1695, 1889, 1994
BshFI GGCC 5 cut(s) 594, 742, 1545, 1819, 2046
BshNI GGYRCC 3 cut(s) 508, 718, 1247
BshVI ATCGAT 1 cut(s) 730
BsiHKAI GWGCWC 1 cut(s) 1099
BsiSI CCGG 1 cut(s) 1816
BslFI GGGAC 1 cut(s) 1882
BslI CCNNNNNNNGG 1 cut(s) 1057
BsmAI GTCTC 4 cut(s) 564, 767, 1661, 2170
BsmBI CGTCTC 1 cut(s) 767
BsmFI GGGAC 1 cut(s) 1882
BsmI GAATGC 2 cut(s) 355, 1907
BsnI GGCC 5 cut(s) 594, 742, 1545, 1819, 2046
Bso31I GGTCTC 1 cut(s) 564
Bsp1286I GDGCHC 1 cut(s) 1099
Bsp1407I TGTACA 1 cut(s) 501
Bsp143I GATC 9 cut(s) 165, 479, 550, 649, 1588, 1843, 1849, 1916, 2160
BspACI CCGC 2 cut(s) 1671, 1715
BspANI GGCC 5 cut(s) 594, 742, 1545, 1819, 2046
BspCNI CTCAG 1 cut(s) 1567
BspDI ATCGAT 1 cut(s) 730
BspLI GGNNCC 7 cut(s) 46, 510, 651, 720, 1168, 1249, 2098
BspMAI CTGCAG 2 cut(s) 550, 823
BspPI GGATC 7 cut(s) 160, 474, 644, 657, 1583, 1851, 2155
BspQI GCTCTTC 2 cut(s) 753, 1702
BspT107I GGYRCC 3 cut(s) 508, 718, 1247
BspTI CTTAAG 1 cut(s) 698
BspTNI GGTCTC 1 cut(s) 564
BsrDI GCAATG 2 cut(s) 459, 1512
BsrFI RCCGGY 1 cut(s) 1815
BsrGI TGTACA 1 cut(s) 501
BsrI ACTGG 5 cut(s) 653, 792, 1071, 1210, 1430
BssAI RCCGGY 1 cut(s) 1815
BssECI CCNNGG 1 cut(s) 1009
BssMI GATC 9 cut(s) 165, 479, 550, 649, 1588, 1843, 1849, 1916, 2160
BssNAI GTATAC 1 cut(s) 61
BssNI GRCGYC 2 cut(s) 509, 776
Bst1107I GTATAC 1 cut(s) 61
Bst2UI CCWGG 2 cut(s) 1011, 2031
Bst4CI ACNGT 8 cut(s) 94, 365, 469, 932, 985, 1041, 1087, 2074
Bst6I CTCTTC 5 cut(s) 753, 1349, 1480, 1525, 1702
BstACI GRCGYC 2 cut(s) 509, 776
BstAFI CTTAAG 1 cut(s) 698
BstAPI GCANNNNNTGC 2 cut(s) 818, 1247
BstAUI TGTACA 1 cut(s) 501
BstBAI YACGTR 2 cut(s) 22, 449
BstC8I GCNNGC 8 cut(s) 596, 744, 1265, 1713, 1817, 1984, 2012, 2048
BstDEI CTNAG 6 cut(s) 170, 1234, 1526, 1575, 1663, 1913
BstF5I GGATG 4 cut(s) 289, 369, 836, 1192
BstH2I RGCGCY 1 cut(s) 512
BstHHI GCGC 2 cut(s) 511, 1815
BstKTI GATC 9 cut(s) 168, 482, 553, 652, 1591, 1846, 1852, 1919, 2163
BstMAI GTCTC 4 cut(s) 564, 767, 1661, 2170
BstMBI GATC 9 cut(s) 165, 479, 550, 649, 1588, 1843, 1849, 1916, 2160
BstMWI GCNNNNNNNGC 6 cut(s) 818, 1247, 1304, 1407, 1724, 1733
BstNI CCWGG 2 cut(s) 1011, 2031
BstNSI RCATGY 2 cut(s) 1136, 1731
BstSCI CCNGG 2 cut(s) 1009, 2029
BstSFI CTRYAG 2 cut(s) 546, 819
BstSLI GKGCMC 1 cut(s) 1099
BstV1I GCAGC 8 cut(s) 340, 1253, 1254, 1410, 1592, 1695, 1889, 1994
BstX2I RGATCY 3 cut(s) 165, 550, 649
BstYI RGATCY 3 cut(s) 165, 550, 649
BstZ17I GTATAC 1 cut(s) 61
Bsu15I ATCGAT 1 cut(s) 730
Bsu36I CCTNAGG 1 cut(s) 1575
BsuRI GGCC 5 cut(s) 594, 742, 1545, 1819, 2046
BsuTUI ATCGAT 1 cut(s) 730
BtgZI GCGATG 1 cut(s) 113
BtsCI GGATG 4 cut(s) 289, 369, 836, 1192
BtsI GCAGTG 1 cut(s) 543
BtsIMutI CAGTG 5 cut(s) 465, 543, 937, 1423, 1601
Cac8I GCNNGC 8 cut(s) 596, 744, 1265, 1713, 1817, 1984, 2012, 2048
CaiI CAGNNNCTG 4 cut(s) 1425, 1574, 1586, 1615
CfoI GCGC 2 cut(s) 511, 1815
Cfr10I RCCGGY 1 cut(s) 1815
Cfr13I GGNCC 3 cut(s) 1166, 1543, 1645
ClaI ATCGAT 1 cut(s) 730
CseI GACGC 1 cut(s) 784
Csp6I GTAC 6 cut(s) 492, 502, 521, 1083, 1731, 1928
CviQI GTAC 6 cut(s) 492, 502, 521, 1083, 1731, 1928
DdeI CTNAG 6 cut(s) 170, 1234, 1526, 1575, 1663, 1913
DinI GGCGCC 1 cut(s) 510
DpnI GATC 9 cut(s) 167, 481, 552, 651, 1590, 1845, 1851, 1918, 2162
DpnII GATC 9 cut(s) 165, 479, 550, 649, 1588, 1843, 1849, 1916, 2160
DraI TTTAAA 1 cut(s) 688
DraIII CACNNNGTG 1 cut(s) 260
EaeI YGGCCR 1 cut(s) 740
Eam1104I CTCTTC 5 cut(s) 753, 1349, 1480, 1525, 1702
EarI CTCTTC 5 cut(s) 753, 1349, 1480, 1525, 1702
Eco31I GGTCTC 1 cut(s) 564
Eco32I GATATC 1 cut(s) 139
Eco47I GGWCC 2 cut(s) 1166, 1645
Eco57I CTGAAG 4 cut(s) 321, 903, 1353, 1551
Eco72I CACGTG 2 cut(s) 22, 449
Eco81I CCTNAGG 1 cut(s) 1575
EcoO109I RGGNCCY 1 cut(s) 1543
EcoRI GAATTC 1 cut(s) 1221
EcoRII CCWGG 2 cut(s) 1009, 2029
EcoRV GATATC 1 cut(s) 139
EcoT22I ATGCAT 2 cut(s) 1480, 1759
EgeI GGCGCC 1 cut(s) 510
EheI GGCGCC 1 cut(s) 510
Esp3I CGTCTC 1 cut(s) 767
FalI AAGNNNNNCTT 2 cut(s) 1182, 1214
FaqI GGGAC 1 cut(s) 1882
FblI GTMKAC 1 cut(s) 60
Fnu4HI GCNGC 9 cut(s) 329, 1242, 1268, 1399, 1581, 1709, 1716, 1878, 2008
FokI GGATG 4 cut(s) 296, 356, 843, 1199
Fsp4HI GCNGC 9 cut(s) 329, 1242, 1268, 1399, 1581, 1709, 1716, 1878, 2008
FspBI CTAG 5 cut(s) 518, 563, 795, 1026, 2052
GlaI GCGC 2 cut(s) 510, 1814
GluI GCNGC 9 cut(s) 329, 1242, 1268, 1399, 1581, 1709, 1716, 1878, 2008
GsuI CTGGAG 2 cut(s) 809, 894
HaeII RGCGCY 1 cut(s) 512
HaeIII GGCC 5 cut(s) 594, 742, 1545, 1819, 2046
HapII CCGG 1 cut(s) 1816
HgaI GACGC 1 cut(s) 784
HhaI GCGC 2 cut(s) 511, 1815
Hin1I GRCGYC 2 cut(s) 509, 776
Hin6I GCGC 2 cut(s) 509, 1813
HinP1I GCGC 2 cut(s) 509, 1813
HincII GTYRAC 1 cut(s) 1384
HindII GTYRAC 1 cut(s) 1384
HindIII AAGCTT 2 cut(s) 1551, 1968
HpaII CCGG 1 cut(s) 1816
HphI GGTGA 5 cut(s) 272, 346, 863, 1815, 2102
Hpy166II GTNNAC 7 cut(s) 61, 263, 459, 904, 1097, 1384, 1645
Hpy8I GTNNAC 7 cut(s) 61, 263, 459, 904, 1097, 1384, 1645
HpyAV CCTTC 9 cut(s) 35, 49, 63, 305, 848, 1261, 1830, 1961, 2196
HpyCH4III ACNGT 8 cut(s) 94, 365, 469, 932, 985, 1041, 1087, 2074
HpyCH4IV ACGT 2 cut(s) 21, 448
HpyF10VI GCNNNNNNNGC 6 cut(s) 818, 1247, 1304, 1407, 1724, 1733
HpyF3I CTNAG 6 cut(s) 170, 1234, 1526, 1575, 1663, 1913
HpySE526I ACGT 2 cut(s) 21, 448
Hsp92I GRCGYC 2 cut(s) 509, 776
HspAI GCGC 2 cut(s) 509, 1813
KasI GGCGCC 1 cut(s) 508
KroI GCCGGC 1 cut(s) 1815
KroNI GCCGGC 1 cut(s) 1817
Kzo9I GATC 9 cut(s) 165, 479, 550, 649, 1588, 1843, 1849, 1916, 2160
LguI GCTCTTC 2 cut(s) 753, 1702
LmnI GCTCC 1 cut(s) 1163
Lsp1109I GCAGC 8 cut(s) 340, 1253, 1254, 1410, 1592, 1695, 1889, 1994
LweI GCATC 5 cut(s) 1145, 1465, 1487, 1744, 1766
MaeI CTAG 5 cut(s) 518, 563, 795, 1026, 2052
MaeII ACGT 2 cut(s) 21, 448
MaeIII GTNAC 7 cut(s) 128, 241, 377, 1140, 1534, 1666, 2065
MalI GATC 9 cut(s) 167, 481, 552, 651, 1590, 1845, 1851, 1918, 2162
MboI GATC 9 cut(s) 165, 479, 550, 649, 1588, 1843, 1849, 1916, 2160
MflI RGATCY 3 cut(s) 165, 550, 649
MhlI GDGCHC 1 cut(s) 1099
MlsI TGGCCA 1 cut(s) 742
MluNI TGGCCA 1 cut(s) 742
Mly113I GGCGCC 1 cut(s) 509
MlyI GAGTC 3 cut(s) 800, 1508, 1542
MmeI TCCRAC 1 cut(s) 384
Mox20I TGGCCA 1 cut(s) 742
Mph1103I ATGCAT 2 cut(s) 1480, 1759
MroNI GCCGGC 1 cut(s) 1815
MroXI GAANNNNTTC 4 cut(s) 309, 1194, 1225, 1358
MscI TGGCCA 1 cut(s) 742
MslI CAYNNNNRTG 2 cut(s) 200, 1313
Msp20I TGGCCA 1 cut(s) 742
MspA1I CMGCKG 2 cut(s) 331, 1244
MspCI CTTAAG 1 cut(s) 698
MspI CCGG 1 cut(s) 1816
MspR9I CCNGG 2 cut(s) 1011, 2031
Mva1269I GAATGC 2 cut(s) 355, 1907
MvaI CCWGG 2 cut(s) 1011, 2031
MwoI GCNNNNNNNGC 6 cut(s) 818, 1247, 1304, 1407, 1724, 1733
NaeI GCCGGC 1 cut(s) 1817
NarI GGCGCC 1 cut(s) 509
NdeII GATC 9 cut(s) 165, 479, 550, 649, 1588, 1843, 1849, 1916, 2160
NgoMIV GCCGGC 1 cut(s) 1815
NlaIV GGNNCC 7 cut(s) 46, 510, 651, 720, 1168, 1249, 2098
NmuCI GTSAC 4 cut(s) 128, 241, 377, 1534
NsiI ATGCAT 2 cut(s) 1480, 1759
NspI RCATGY 2 cut(s) 1136, 1731
PciI ACATGT 1 cut(s) 1132
PciSI GCTCTTC 2 cut(s) 753, 1702
PctI GAATGC 2 cut(s) 355, 1907
PdiI GCCGGC 1 cut(s) 1817
PdmI GAANNNNTTC 4 cut(s) 309, 1194, 1225, 1358
PfeI GAWTC 7 cut(s) 209, 974, 1014, 1175, 1612, 1618, 1856
PflMI CCANNNNNTGG 1 cut(s) 1057
PkrI GCNGC 9 cut(s) 330, 1243, 1269, 1400, 1582, 1710, 1717, 1879, 2009
PleI GAGTC 3 cut(s) 799, 1507, 1541
PluTI GGCGCC 1 cut(s) 512
PmaCI CACGTG 2 cut(s) 22, 449
PmlI CACGTG 2 cut(s) 22, 449
PpsI GAGTC 3 cut(s) 799, 1507, 1541
Ppu21I YACGTR 2 cut(s) 22, 449
PscI ACATGT 1 cut(s) 1132
Psp6I CCWGG 2 cut(s) 1009, 2029
PspCI CACGTG 2 cut(s) 22, 449
PspGI CCWGG 2 cut(s) 1009, 2029
PspN4I GGNNCC 7 cut(s) 46, 510, 651, 720, 1168, 1249, 2098
PspPI GGNCC 3 cut(s) 1166, 1543, 1645
PstI CTGCAG 2 cut(s) 550, 823
PstNI CAGNNNCTG 4 cut(s) 1425, 1574, 1586, 1615
PsuI RGATCY 3 cut(s) 165, 550, 649
PvuII CAGCTG 2 cut(s) 331, 1244
RsaI GTAC 6 cut(s) 493, 503, 522, 1084, 1732, 1929
RsaNI GTAC 6 cut(s) 492, 502, 521, 1083, 1731, 1928
RseI CAYNNNNRTG 2 cut(s) 200, 1313
SapI GCTCTTC 2 cut(s) 753, 1702
SatI GCNGC 9 cut(s) 329, 1242, 1268, 1399, 1581, 1709, 1716, 1878, 2008
Sau3AI GATC 9 cut(s) 165, 479, 550, 649, 1588, 1843, 1849, 1916, 2160
Sau96I GGNCC 3 cut(s) 1166, 1543, 1645
SchI GAGTC 3 cut(s) 800, 1508, 1542
ScrFI CCNGG 2 cut(s) 1011, 2031
SduI GDGCHC 1 cut(s) 1099
SfaNI GCATC 5 cut(s) 1145, 1465, 1487, 1744, 1766
SfcI CTRYAG 2 cut(s) 546, 819
SfoI GGCGCC 1 cut(s) 510
SinI GGWCC 2 cut(s) 1166, 1645
SmiMI CAYNNNNRTG 2 cut(s) 200, 1313
SmlI CTYRAG 3 cut(s) 278, 698, 957
SmoI CTYRAG 3 cut(s) 278, 698, 957
SpeI ACTAGT 1 cut(s) 517
SsiI CCGC 2 cut(s) 1671, 1715
SspDI GGCGCC 1 cut(s) 508
SspI AATATT 1 cut(s) 573
SspMI CTAG 5 cut(s) 518, 563, 795, 1026, 2052
StyD4I CCNGG 2 cut(s) 1009, 2029
TaaI ACNGT 8 cut(s) 94, 365, 469, 932, 985, 1041, 1087, 2074
TaiI ACGT 2 cut(s) 24, 451
TaqI TCGA 2 cut(s) 9, 730
TatI WGTACW 2 cut(s) 501, 1927
TauI GCSGC 1 cut(s) 1718
TfiI GAWTC 7 cut(s) 209, 974, 1014, 1175, 1612, 1618, 1856
TscAI CASTG 5 cut(s) 472, 550, 937, 1430, 1608
TseFI GTSAC 4 cut(s) 128, 241, 377, 1534
TseI GCWGC 8 cut(s) 328, 1241, 1267, 1398, 1580, 1708, 1877, 2007
Tsp45I GTSAC 4 cut(s) 128, 241, 377, 1534
TspRI CASTG 5 cut(s) 472, 550, 937, 1430, 1608
Van91I CCANNNNNTGG 1 cut(s) 1057
Vha464I CTTAAG 1 cut(s) 698
VneI GTGCAC 1 cut(s) 1095
VpaK11BI GGWCC 2 cut(s) 1166, 1645
XapI RAATTY 6 cut(s) 104, 248, 273, 1053, 1221, 1374
XceI RCATGY 2 cut(s) 1136, 1731
XmiI GTMKAC 1 cut(s) 60
XmnI GAANNNNTTC 4 cut(s) 309, 1194, 1225, 1358
XspI CTAG 5 cut(s) 518, 563, 795, 1026, 2052
Zsp2I ATGCAT 2 cut(s) 1480, 1759
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.