RchiOBHm_Chr1g0383891

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0004502
Physical Location & Seq
Forward (+)
68017500 .. 68026576
9077 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ60677

Sequence Viewer

Length: 4008 bp
ATGGGTGTCGAGAATTATCATGTAATTGAGCTTGTAGGCGAAGGTTCCTTTGGGAAGGTATACAAGGGAAGACGCAAGTTCACAGGCCAGACCGTTGCAATGAAATTTATTATGAAACACGGGAAAAGCGATAAGGATATCCACAATTTAAGACAAGAAATAGAGATTCTAAGGAAGCTAAAGCATGAAAATATCATTGAAATGCTTGATTCCTTTGAAAGCCCACAAGAATTCTGTGTTGTCACAGAGTTTGCACAAGGTGAACTATTTGAAATTCTTGAAGATGATAAGCACCTTCCAGAAGAACAAGTTCAAGCAATTGCTAAGCAATTGGTGAGAGCATTGCATTATCTCCATTCAAACCGTATCATCCATCGTGACATGAAACCACAAAACATTCTCATTGGTGCTGGTTCTATTGTTAAGCTCTGTGACTTTGGGTTTGCACGTGCAATGTCCACAAATACTGTTGTTTTGCGATCCATAAAAGGTACTCCCCTGTACATGGCTCCTGAACTAGTCCGGGAACAACCTTACAACCACACTGCAGATTTATGGTCTCTCGGAGTTATATTATATGAATTATTTGTTGGCCAGCCTCCATTTTATACAAATTCAGTATATGCACTCATTCGGCATATTGTGAAGGATCCAGTTAAATACCCTGATAACATGAGTTCAAATTTTAAAAACTTTCTGAAGGGATTGCTCAATAAGATGCCTCAAAATCGTTTGACTTGGCCAGCTCTTTTGGAACACCCATTTGTTAAGGAGACAACTGAAGAACTGGAGGCCAGGGAAATGCGTTCTGCTACTTCTGCAGAAAGGGGGTGCGTTGCAGCATGGAGGGGTGAAGGAAACAAAATCCAAACATCAGGTGGCTTGGCTGTTTCTAGTCCTGTCAACTCTCCAGCTTCTTCAGAGAACAACAGTGGAATAAGTTTCCAAAATGATGCTCAAACAAATATTCCAGACTCAAAAGCAGTCAATTCTTCTCCAAATGAGGAGTTTCCAGGGTTTGCAGATCCTGATAAAGTTAAACAGTCAGGTTGTCAAATTCTCGACAGACTGGAAAATAACTCTCGTACAGTTAAAGGTGCACAAATAATTGGTCAAGATAATGAAGCATTAGCACATGTTTTGCTACCACTAAAAAGATGCTCGCAAGGATCCCAAAACTCTAGCAGGGATCAAGATATTCTTAGTTCAAACCAGTCTTTAAGAATCCTTTCAAACTTAGTTGCAGCTGGAGCCATCACTTCCAGTGGGCTAGTTGATGAAATCATACATGAAATTCTTGTTTATACCACCTTCATTGTGGGCATCAAATCTTCTGAACTGAATGAGTTTAGAGCAAAGAGTTTCTCAGTAATTAAAGTTTTGGTAGACATTGCTGGACATGGCATTGGAGGCTCATATTTCAGGCACTGGGTTGCTTTAGCTGATATTTTTTCACAGGTTGTTGGTGGCAGTGAAGATGCATCTGGAAGAGTTTTGTTTGAGTCCATAGCTTGCATTACTGTCATGTTAACAAGAGTTAATCAGGGTCTGAAACAGGCTGTTTCTGATCCTAATGAAGCAGTGAAACAGATTTTGGATCATGCTAAAACATCTGGTTTAGTGGATCAGCTTTGTCTCTGCTTAGCTACTGCTGGAGCAAGTCTTATTTCAGGTCCTTCAAATATGTCGCGTTCAGCCTGTGAAGCATGCAGGGCTATTTGGTTGCTGGTAGATGCATCAGAGTTTTTTTCAACAAAAGGAAATGCCATTTCCTTTCCGCTAAACACTTTGCGCAGTTCATCAAGGGCTCAAGATCGAGGAGGTTCTCAGATTGGAACAGAATCAGCAAGACTAGTAGCTGTAGTTACTAGAGCATTAGTTAGATCTAAACCAGTTCAGGTTGCCATCCATTATTGCCTTCGTCAACGTCTTGAGGCTTCACTCTGTGCTGGTATTCAGCTATTGTTGAGGTGCTGCTTGCAGAGTGGAATTGTTCCTGGCATTCTTTGTGGCCTGCCTAGTTCTTTACCTGTAACTACTGTTGTCAGTGGTGGAGGTGATGGAACCATAATTTTTGAGATATTCTCTTTGTTGTCTGTGTGCATTTCATCTCAAAATAAAGATCCACAAGCAATTGAGACAACCAACTTGAAGAGCAAAATAACCGATCCCAATATGTTGGTTCTGCATTCCTGCCTCATCCTTGCATCAGTTGCACAGTGTTTAAAGGCAACTGGAAGAAATTCTGCATTGTTTATGCTTACTACATCCTCGAAGAAGCAGCTTTCTCGACTTTCTGCCCTTGCTCATCACTTTTCTTCCGGTGAGAGCACAAATACTTCCTCTCAAACTCACAGTGCAGCAGCCATGTTGGCACTGGCATCTATTTTGTCCCTTGAATCTGGAGCTTCTGTTGGGTCCTCTGTCTTTGAGGTAGCAGTGCCTTTGATTCCTCAAACCGCGACACTTTGTGAATGCCTCAAACTTTCATCAAACAGTGAGATTGTATCGGGTCCTAATGGCACCAACGGTGTTCTCTCAAACTGGCATGGTCTTAGGGATGGATGTGTTGGCCTGCTGGAGGCCAGACTGAGATGGGGGGGACCCTTGGCTGTTCAACAACTTTGTGCAAGCAATATCCCTCTGCTTCTAATCAATTTGTTAGCCAAAAACCAACAAAATGCTTCTCCTCAAGAAGTTGCCAGCACAAATGATCAAGTTGGGCTTTCCCCTATTGGAGTTGTATGGACAGTATCATCTATTTGTCAGTGCCTTTCTGGTGGAGCCTTAACCTTTCGTCAGATTCTGCTTAGAAGTGAGCATATCAAACTCCTCTCTGACTTGATATCTGACATGCATCTCAAACTTGTAAAGTCCTGGGTTGGGCCTGGTGGAGGAATGGATGGCGTAAGAGATATAACAAACGCAGTAATTGACCTCTTGGCATTTCCTTTTGTGGCTGTTCAGAATGCTCCAGGATTGCCATCAGCGACTGCCTCCGTGAACAGTGGGATCCTTCTGAACATGGGTTCACCAGGTGTGAAAGTTGGCGTGGAAGACAGAGACATGGTGAAGGTGATTGAGGAAGACCTCGGAAAATATATTAAAATCCTTTTGGAGGTTGGAGTGCCTGGTATCATTCTTTGGTGTCTAGAACATTTGGAGTTAAAAGATTTGGGAAGACCTGTGGCCTTTCTGGCTAAAATGATTGGTCACCGACCATTGGCAGTTCAACTTGTGGGTAAAGGCTTGTTGGATCCTACTAGGATGAGAAGATTGCTCGATCGTTCAAGCCCAAAAGAAGTCATGCTGGATGTTCTGATGATTGTTTCTGATTTGGCACGCATGGACAAGGGATTCTATGAATACATTAATCGAGCTTCTCTGCTGGAGTTCTTCAAGGGATTTCTTACCCATGAAGATCCTAGTGTGCGTTCGAAGGCTTGCAGTGCTCTAGGCAATATGTGCCGGCACAGCTCCTACTTTTACAGTTCCCTAGCAAAGAATCAAATCATTGGTCTCCTTATTGATCGATGCTCTGACCCAGACAAACGTACACGGAAATTTGCTTGTTTTGCTATTGGCAATGCCGCTTACCATAATGATATGTTGTATGAAGAGCTGCGGAGATCTATCCCCCAGCTTGCCAATTTGTTGCTTTCATCTGAGGAAGACAAGACTAAAGCAAATGCAGCAGGTGCACTAAGCAATCTTATCCGCAACTCCAACAAACTTTGCGAAGACATTGTCTCTAGAGGAGCCATGCAGTCTTTGCTGAAGTTGGTGGCAGAGTGCTCAGTGTTGGCTCTGAACCCAAGTAGGAAAGATTCAGCGCAAGAGTCACCTCTCAAAATAGCTCTGTTTTCATTGGCAAAAATGTGCTCGCATCCGCCCTGCAGAGATTTCCTCCGTTCATCTGAGTTGTTCCCCGTATTCGGACGGCTCCGCCAATCCCCGGAATCAACAATTGCCAACTACGCCTCCGCCATCATCAACAAAGTTGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000166 GO:0000278 GO:0000280 GO:0000281 GO:0000910 GO:0000911 GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005856 GO:0005874 GO:0005881 GO:0006355 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006935 GO:0006996 GO:0007049 GO:0007112 GO:0007140 GO:0007154 GO:0007163 GO:0007165 GO:0007166 GO:0007224 GO:0007227 GO:0007228 GO:0007275 GO:0007276 GO:0007349 GO:0007584 GO:0008064 GO:0008092 GO:0008134 GO:0008144 GO:0008150 GO:0008152 GO:0008589 GO:0009524 GO:0009553 GO:0009558 GO:0009561 GO:0009605 GO:0009653 GO:0009791 GO:0009889 GO:0009966 GO:0009967 GO:0009987 GO:0009991 GO:0010033 GO:0010243 GO:0010468 GO:0010556 GO:0010639 GO:0010646 GO:0010647 GO:0014070 GO:0015630 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017076 GO:0019219 GO:0019222 GO:0019538 GO:0019894 GO:0019953 GO:0022402 GO:0022412 GO:0022414 GO:0022607 GO:0023051 GO:0023052 GO:0023056 GO:0030010 GO:0030030 GO:0030031 GO:0030554 GO:0030832 GO:0030833 GO:0030837 GO:0031156 GO:0031272 GO:0031323 GO:0031326 GO:0031333 GO:0031344 GO:0031667 GO:0032101 GO:0032104 GO:0032107 GO:0032271 GO:0032272 GO:0032501 GO:0032502 GO:0032504 GO:0032506 GO:0032535 GO:0032553 GO:0032555 GO:0032559 GO:0032956 GO:0032970 GO:0033043 GO:0033206 GO:0033273 GO:0035556 GO:0035639 GO:0036094 GO:0036211 GO:0040011 GO:0042221 GO:0042330 GO:0042493 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043254 GO:0043326 GO:0043327 GO:0043412 GO:0043900 GO:0044085 GO:0044087 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0044703 GO:0044782 GO:0045880 GO:0048229 GO:0048232 GO:0048285 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048609 GO:0048646 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051090 GO:0051091 GO:0051128 GO:0051129 GO:0051171 GO:0051252 GO:0051301 GO:0051321 GO:0051493 GO:0051494 GO:0051593 GO:0051704 GO:0051716 GO:0060176 GO:0060255 GO:0060271 GO:0060491 GO:0061640 GO:0065007 GO:0065008 GO:0065009 GO:0070925 GO:0071704 GO:0071840 GO:0080090 GO:0080134 GO:0080135 GO:0090066 GO:0097159 GO:0097367 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0110053 GO:0120031 GO:0120032 GO:0120035 GO:0120036 GO:0140013 GO:0140096 GO:1901265 GO:1901363 GO:1901564 GO:1901698 GO:1901700 GO:1902410 GO:1902903 GO:1902904 GO:1903046 GO:1903047 GO:1903506 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04341 map04341
Pfam Domains
Protein Families
Arm HEAT HEAT_2 Pkinase

Protein Analysis

1335

Amino Acids

145.04

Weight (kDa)

8.3

Isoelectric Point (pI)

49.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 6 - 256 1.1e-78 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 8 - 251 3.4e-47 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 3686
Acc16I TGCGCA 1 cut(s) 1793
Acc36I ACCTGC 1 cut(s) 3686
AccB1I GGYRCC 1 cut(s) 2522
AccI GTMKAC 2 cut(s) 60, 1386
AccII CGCG 2 cut(s) 1690, 2462
AciI CCGC 8 cut(s) 1778, 2460, 3591, 3625, 3718, 3890, 3946, 3984
AcoI YGGCCR 2 cut(s) 592, 740
AcsI RAATTY 9 cut(s) 104, 230, 273, 613, 682, 1056, 1293, 2242, 3563
AcuI CTGAAG 4 cut(s) 719, 801, 903, 3797
AcvI CACGTG 1 cut(s) 449
AdeI CACNNNGTG 4 cut(s) 260, 1946, 2471, 3038
AfaI GTAC 4 cut(s) 493, 503, 1087, 3556
AfiI CCNNNNNNNGG 8 cut(s) 505, 2581, 2883, 2894, 3118, 3223, 3935, 3955
AflIII ACRYGT 1 cut(s) 1135
AhlI ACTAGT 2 cut(s) 517, 1852
AjnI CCWGG 8 cut(s) 794, 1012, 1996, 2876, 2887, 2974, 3034, 3130
AjuI GAANNNNNNNTTGG 6 cut(s) 33, 65, 573, 605, 1577, 1609
Alw21I GWGCWC 6 cut(s) 1102, 2333, 3454, 3703, 3797, 3884
Alw26I GTCTC 7 cut(s) 564, 767, 1640, 2132, 3057, 3524, 3754
Alw44I GTGCAC 2 cut(s) 1098, 3699
AlwNI CAGNNNCTG 5 cut(s) 1028, 1428, 1549, 2806, 2993
AoxI GGCC 9 cut(s) 85, 592, 740, 792, 2011, 2572, 2583, 2885, 3189
ApaLI GTGCAC 2 cut(s) 1098, 3699
ApeKI GCWGC 8 cut(s) 839, 1244, 1974, 2281, 2360, 2363, 3622, 3692
ApoI RAATTY 9 cut(s) 104, 230, 273, 613, 682, 1056, 1293, 2242, 3563
ArsI GACNNNNNNTTYG 8 cut(s) 425, 457, 1096, 1128, 3502, 3534, 3732, 3764
AseI ATTAAT 1 cut(s) 3372
Asp700I GAANNNNTTC 3 cut(s) 309, 1228, 2242
AspLEI GCGC 2 cut(s) 1794, 3835
AspS9I GGNCC 5 cut(s) 1673, 2418, 2513, 2603, 2885
AsuC2I CCSGG 2 cut(s) 524, 3956
AsuII TTCGAA 1 cut(s) 3437
AvaII GGWCC 4 cut(s) 1673, 2418, 2513, 2603
BaeGI GKGCMC 2 cut(s) 1102, 3703
BalI TGGCCA 2 cut(s) 594, 742
BamHI GGATCC 4 cut(s) 649, 1169, 3012, 3256
BanI GGYRCC 1 cut(s) 2522
BanII GRGCYC 1 cut(s) 1810
BbrPI CACGTG 1 cut(s) 449
BbsI GAAGAC 6 cut(s) 76, 3063, 3093, 3187, 3678, 3747
Bbv12I GWGCWC 6 cut(s) 1102, 2333, 3454, 3703, 3797, 3884
BbvI GCAGC 8 cut(s) 851, 1256, 1961, 2293, 2372, 2375, 3609, 3704
BccI CCATC 9 cut(s) 381, 1262, 1913, 2054, 2555, 2589, 2897, 2992, 3995
BceAI ACGGC 1 cut(s) 3956
BcgI CGANNNNNNTGC 2 cut(s) 2270, 2304
BciT130I CCWGG 8 cut(s) 796, 1014, 1998, 2878, 2889, 2976, 3036, 3132
BclI TGATCA 1 cut(s) 2713
BcnI CCSGG 2 cut(s) 524, 3956
BcoDI GTCTC 7 cut(s) 564, 767, 1640, 2132, 3057, 3524, 3754
BcuI ACTAGT 2 cut(s) 517, 1852
BfmI CTRYAG 4 cut(s) 546, 819, 1860, 3895
BfuAI ACCTGC 1 cut(s) 3686
BglI GCCNNNNNGGC 2 cut(s) 2372, 3197
BglII AGATCT 2 cut(s) 1883, 3629
BisI GCNGC 9 cut(s) 840, 1245, 1975, 2282, 2361, 2364, 3591, 3623, 3693
BlpI GCTNAGC 2 cut(s) 324, 1642
BlsI GCNGC 9 cut(s) 841, 1246, 1976, 2283, 2362, 2365, 3592, 3624, 3694
Bme18I GGWCC 4 cut(s) 1673, 2418, 2513, 2603
BmgT120I GGNCC 5 cut(s) 1673, 2418, 2513, 2603, 2885
BmrI ACTGGG 1 cut(s) 1438
BmuI ACTGGG 1 cut(s) 1438
BpiI GAAGAC 6 cut(s) 76, 3063, 3093, 3187, 3678, 3747
BplI GAGNNNNNCTC 4 cut(s) 2069, 2101, 3891, 3923
BpmI CTGGAG 8 cut(s) 809, 894, 1269, 1674, 2424, 2600, 2958, 3410
Bpu1102I GCTNAGC 2 cut(s) 324, 1642
Bpu14I TTCGAA 1 cut(s) 3437
BpuEI CTTGAG 3 cut(s) 1794, 1952, 2676
BpuMI CCSGG 2 cut(s) 524, 3956
Bsa29I ATCGAT 1 cut(s) 3532
BsaAI YACGTR 1 cut(s) 449
BsaBI GATNNNNATC 1 cut(s) 2983
BsaI GGTCTC 2 cut(s) 564, 3524
BsaJI CCNNGG 6 cut(s) 795, 1013, 2607, 2877, 3091, 3954
BsaWI WCCGGW 1 cut(s) 2321
BsaXI ACNNNNNCTCC 4 cut(s) 2397, 2427, 3965, 3995
Bsc4I CCNNNNNNNGG 8 cut(s) 505, 2581, 2883, 2894, 3118, 3223, 3935, 3955
Bse118I RCCGGY 1 cut(s) 3468
Bse3DI GCAATG 5 cut(s) 105, 341, 459, 1389, 3592
Bse8I GATNNNNATC 1 cut(s) 2983
BseBI CCWGG 8 cut(s) 796, 1014, 1998, 2878, 2889, 2976, 3036, 3132
BseCI ATCGAT 1 cut(s) 3532
BseDI CCNNGG 6 cut(s) 795, 1013, 2607, 2877, 3091, 3954
BseJI GATNNNNATC 1 cut(s) 2983
BseLI CCNNNNNNNGG 8 cut(s) 505, 2581, 2883, 2894, 3118, 3223, 3935, 3955
BseMI GCAATG 5 cut(s) 105, 341, 459, 1389, 3592
BseMII CTCAG 6 cut(s) 1380, 1841, 2582, 3657, 3810, 3909
BseRI GAGGAG 5 cut(s) 1019, 1833, 2679, 2822, 3771
BseSI GKGCMC 2 cut(s) 1102, 3703
BseXI GCAGC 8 cut(s) 851, 1256, 1961, 2293, 2372, 2375, 3609, 3704
BseYI CCCAGC 1 cut(s) 3639
BsgI GTGCAG 1 cut(s) 2379
Bsh1236I CGCG 2 cut(s) 1690, 2462
Bsh1285I CGRYCG 1 cut(s) 3286
BshFI GGCC 9 cut(s) 87, 594, 742, 794, 2013, 2574, 2585, 2887, 3191
BshNI GGYRCC 1 cut(s) 2522
BshVI ATCGAT 1 cut(s) 3532
BsiEI CGRYCG 1 cut(s) 3286
BsiHKAI GWGCWC 6 cut(s) 1102, 2333, 3454, 3703, 3797, 3884
BsiSI CCGG 4 cut(s) 523, 2322, 3469, 3956
BslFI GGGAC 2 cut(s) 2377, 2616
BslI CCNNNNNNNGG 8 cut(s) 505, 2581, 2883, 2894, 3118, 3223, 3935, 3955
BsmAI GTCTC 7 cut(s) 564, 767, 1640, 2132, 3057, 3524, 3754
BsmFI GGGAC 2 cut(s) 2377, 2616
BsmI GAATGC 4 cut(s) 2001, 2188, 2480, 2974
BsnI GGCC 9 cut(s) 87, 594, 742, 794, 2013, 2574, 2585, 2887, 3191
Bso31I GGTCTC 2 cut(s) 564, 3524
Bsp119I TTCGAA 1 cut(s) 3437
Bsp1286I GDGCHC 7 cut(s) 1102, 1810, 2333, 3454, 3703, 3797, 3884
Bsp1407I TGTACA 1 cut(s) 501
Bsp1720I GCTNAGC 2 cut(s) 324, 1642
BspACI CCGC 8 cut(s) 1778, 2460, 3591, 3625, 3718, 3890, 3946, 3984
BspANI GGCC 9 cut(s) 87, 594, 742, 794, 2013, 2574, 2585, 2887, 3191
BspCNI CTCAG 6 cut(s) 1379, 1840, 2583, 3658, 3809, 3910
BspDI ATCGAT 1 cut(s) 3532
BspFNI CGCG 2 cut(s) 1690, 2462
BspMAI CTGCAG 3 cut(s) 550, 823, 3899
BspMI ACCTGC 1 cut(s) 3686
BspQI GCTCTTC 2 cut(s) 2147, 3612
BspT104I TTCGAA 1 cut(s) 3437
BspT107I GGYRCC 1 cut(s) 2522
BspTNI GGTCTC 2 cut(s) 564, 3524
BsrDI GCAATG 5 cut(s) 105, 341, 459, 1389, 3592
BsrFI RCCGGY 1 cut(s) 3468
BsrGI TGTACA 1 cut(s) 501
BssAI RCCGGY 1 cut(s) 3468
BssECI CCNNGG 6 cut(s) 795, 1013, 2607, 2877, 3091, 3954
BssNAI GTATAC 1 cut(s) 61
BssT1I CCWWGG 1 cut(s) 2607
Bst1107I GTATAC 1 cut(s) 61
Bst2UI CCWGG 8 cut(s) 796, 1014, 1998, 2878, 2889, 2976, 3036, 3132
Bst6I CTCTTC 3 cut(s) 1483, 2147, 3612
BstAPI GCANNNNNTGC 4 cut(s) 2213, 3465, 3698, 3772
BstAUI TGTACA 1 cut(s) 501
BstBAI YACGTR 1 cut(s) 449
BstBI TTCGAA 1 cut(s) 3437
BstEII GGTNACC 1 cut(s) 3212
BstENI CCTNNNNNAGG 3 cut(s) 2579, 2892, 3116
BstFNI CGCG 2 cut(s) 1690, 2462
BstHHI GCGC 2 cut(s) 1794, 3835
BstMAI GTCTC 7 cut(s) 564, 767, 1640, 2132, 3057, 3524, 3754
BstMCI CGRYCG 1 cut(s) 3286
BstNI CCWGG 8 cut(s) 796, 1014, 1998, 2878, 2889, 2976, 3036, 3132
BstNSI RCATGY 3 cut(s) 1139, 1710, 2857
BstPI GGTNACC 1 cut(s) 3212
BstSFI CTRYAG 4 cut(s) 546, 819, 1860, 3895
BstSLI GKGCMC 2 cut(s) 1102, 3703
BstUI CGCG 2 cut(s) 1690, 2462
BstV1I GCAGC 8 cut(s) 851, 1256, 1961, 2293, 2372, 2375, 3609, 3704
BstV2I GAAGAC 6 cut(s) 76, 3063, 3093, 3187, 3678, 3747
BstX2I RGATCY 9 cut(s) 649, 1024, 1169, 1883, 2122, 3012, 3256, 3421, 3629
BstXI CCANNNNNNTGG 1 cut(s) 2179
BstYI RGATCY 9 cut(s) 649, 1024, 1169, 1883, 2122, 3012, 3256, 3421, 3629
BstZ17I GTATAC 1 cut(s) 61
Bsu15I ATCGAT 1 cut(s) 3532
BsuRI GGCC 9 cut(s) 87, 594, 742, 794, 2013, 2574, 2585, 2887, 3191
BsuTUI ATCGAT 1 cut(s) 3532
BtsI GCAGTG 5 cut(s) 543, 1477, 1587, 2445, 3454
BveI ACCTGC 1 cut(s) 3686
CaiI CAGNNNCTG 5 cut(s) 1028, 1428, 1549, 2806, 2993
CfoI GCGC 2 cut(s) 1794, 3835
Cfr10I RCCGGY 1 cut(s) 3468
Cfr13I GGNCC 5 cut(s) 1673, 2418, 2513, 2603, 2885
ClaI ATCGAT 1 cut(s) 3532
CseI GACGC 1 cut(s) 81
CsiI ACCWGGT 1 cut(s) 3034
Csp6I GTAC 4 cut(s) 492, 502, 1086, 3555
CspCI CAANNNNNGTGG 2 cut(s) 1297, 1332
CviQI GTAC 4 cut(s) 492, 502, 1086, 3555
DraI TTTAAA 2 cut(s) 688, 2226
DraIII CACNNNGTG 4 cut(s) 260, 1946, 2471, 3038
EaeI YGGCCR 2 cut(s) 592, 740
Eam1104I CTCTTC 3 cut(s) 1483, 2147, 3612
EarI CTCTTC 3 cut(s) 1483, 2147, 3612
EciI GGCGGA 3 cut(s) 3879, 3935, 3973
Eco130I CCWWGG 1 cut(s) 2607
Eco24I GRGCYC 1 cut(s) 1810
Eco31I GGTCTC 2 cut(s) 564, 3524
Eco32I GATATC 2 cut(s) 139, 2847
Eco47I GGWCC 4 cut(s) 1673, 2418, 2513, 2603
Eco57I CTGAAG 4 cut(s) 719, 801, 903, 3797
Eco72I CACGTG 1 cut(s) 449
Eco91I GGTNACC 1 cut(s) 3212
EcoNI CCTNNNNNAGG 3 cut(s) 2579, 2892, 3116
EcoO109I RGGNCCY 4 cut(s) 1673, 2418, 2513, 2603
EcoO65I GGTNACC 1 cut(s) 3212
EcoRI GAATTC 1 cut(s) 230
EcoRII CCWGG 8 cut(s) 794, 1012, 1996, 2876, 2887, 2974, 3034, 3130
EcoRV GATATC 2 cut(s) 139, 2847
EcoT14I CCWWGG 1 cut(s) 2607
EcoT22I ATGCAT 3 cut(s) 1483, 1738, 2859
EcoT38I GRGCYC 1 cut(s) 1810
ErhI CCWWGG 1 cut(s) 2607
FalI AAGNNNNNCTT 4 cut(s) 1185, 1217, 2709, 2741
FaqI GGGAC 2 cut(s) 2377, 2616
FbaI TGATCA 1 cut(s) 2713
FblI GTMKAC 2 cut(s) 60, 1386
Fnu4HI GCNGC 9 cut(s) 840, 1245, 1975, 2282, 2361, 2364, 3591, 3623, 3693
FriOI GRGCYC 1 cut(s) 1810
Fsp4HI GCNGC 9 cut(s) 840, 1245, 1975, 2282, 2361, 2364, 3591, 3623, 3693
FspI TGCGCA 1 cut(s) 1793
GlaI GCGC 2 cut(s) 1793, 3834
GluI GCNGC 9 cut(s) 840, 1245, 1975, 2282, 2361, 2364, 3591, 3623, 3693
GsaI CCCAGC 1 cut(s) 3643
GsuI CTGGAG 8 cut(s) 809, 894, 1269, 1674, 2424, 2600, 2958, 3410
HaeIII GGCC 9 cut(s) 87, 594, 742, 794, 2013, 2574, 2585, 2887, 3191
HapII CCGG 4 cut(s) 523, 2322, 3469, 3956
HgaI GACGC 1 cut(s) 81
HhaI GCGC 2 cut(s) 1794, 3835
Hin6I GCGC 2 cut(s) 1792, 3833
HinP1I GCGC 2 cut(s) 1792, 3833
HincII GTYRAC 3 cut(s) 904, 1530, 1925
HindII GTYRAC 3 cut(s) 904, 1530, 1925
HpaI GTTAAC 1 cut(s) 1530
HpaII CCGG 4 cut(s) 523, 2322, 3469, 3956
HpyCH4IV ACGT 3 cut(s) 448, 1927, 3553
HpySE526I ACGT 3 cut(s) 448, 1927, 3553
HspAI GCGC 2 cut(s) 1792, 3833
KflI GGGWCCC 1 cut(s) 2603
KroI GCCGGC 1 cut(s) 3468
KroNI GCCGGC 1 cut(s) 3470
Ksp22I TGATCA 1 cut(s) 2713
KspAI GTTAAC 1 cut(s) 1530
LguI GCTCTTC 2 cut(s) 2147, 3612
LmnI GCTCC 9 cut(s) 514, 1250, 1655, 2405, 2783, 2977, 3482, 3758, 3948
Lsp1109I GCAGC 8 cut(s) 851, 1256, 1961, 2293, 2372, 2375, 3609, 3704
MabI ACCWGGT 1 cut(s) 3034
MaeII ACGT 3 cut(s) 448, 1927, 3553
MaeIII GTNAC 7 cut(s) 241, 377, 431, 1864, 2032, 3212, 3840
MfeI CAATTG 4 cut(s) 318, 329, 2133, 3966
MflI RGATCY 9 cut(s) 649, 1024, 1169, 1883, 2122, 3012, 3256, 3421, 3629
MhlI GDGCHC 7 cut(s) 1102, 1810, 2333, 3454, 3703, 3797, 3884
MlsI TGGCCA 2 cut(s) 594, 742
MluNI TGGCCA 2 cut(s) 594, 742
MlyI GAGTC 3 cut(s) 968, 1511, 3848
MmeI TCCRAC 3 cut(s) 3103, 3234, 3750
Mox20I TGGCCA 2 cut(s) 594, 742
Mph1103I ATGCAT 3 cut(s) 1483, 1738, 2859
MroNI GCCGGC 1 cut(s) 3468
MroXI GAANNNNTTC 3 cut(s) 309, 1228, 2242
MscI TGGCCA 2 cut(s) 594, 742
MslI CAYNNNNRTG 1 cut(s) 200
Msp20I TGGCCA 2 cut(s) 594, 742
MspA1I CMGCKG 1 cut(s) 1247
MspI CCGG 4 cut(s) 523, 2322, 3469, 3956
MunI CAATTG 4 cut(s) 318, 329, 2133, 3966
Mva1269I GAATGC 4 cut(s) 2001, 2188, 2480, 2974
MvaI CCWGG 8 cut(s) 796, 1014, 1998, 2878, 2889, 2976, 3036, 3132
MvnI CGCG 2 cut(s) 1690, 2462
NaeI GCCGGC 1 cut(s) 3470
NciI CCSGG 2 cut(s) 524, 3956
NgoMIV GCCGGC 1 cut(s) 3468
NmuCI GTSAC 5 cut(s) 241, 377, 431, 3212, 3840
NsbI TGCGCA 1 cut(s) 1793
NsiI ATGCAT 3 cut(s) 1483, 1738, 2859
NspI RCATGY 3 cut(s) 1139, 1710, 2857
NspV TTCGAA 1 cut(s) 3437
PaeI GCATGC 1 cut(s) 1710
PaqCI CACCTGC 1 cut(s) 3686
PciI ACATGT 1 cut(s) 1135
PciSI GCTCTTC 2 cut(s) 2147, 3612
PcsI WCGNNNNNNNCGW 1 cut(s) 126
PctI GAATGC 4 cut(s) 2001, 2188, 2480, 2974
PdiI GCCGGC 1 cut(s) 3470
PdmI GAANNNNTTC 3 cut(s) 309, 1228, 2242
PflFI GACNNNGTC 1 cut(s) 3746
PfoI TCCNGGA 2 cut(s) 522, 2974
PkrI GCNGC 9 cut(s) 841, 1246, 1976, 2283, 2362, 2365, 3592, 3624, 3694
Ple19I CGATCG 1 cut(s) 3286
PleI GAGTC 3 cut(s) 968, 1510, 3847
PmaCI CACGTG 1 cut(s) 449
PmlI CACGTG 1 cut(s) 449
PpsI GAGTC 3 cut(s) 968, 1510, 3847
Ppu21I YACGTR 1 cut(s) 449
PpuMI RGGWCCY 4 cut(s) 1673, 2418, 2513, 2603
PscI ACATGT 1 cut(s) 1135
PshBI ATTAAT 1 cut(s) 3372
Psp5II RGGWCCY 4 cut(s) 1673, 2418, 2513, 2603
Psp6I CCWGG 8 cut(s) 794, 1012, 1996, 2876, 2887, 2974, 3034, 3130
PspCI CACGTG 1 cut(s) 449
PspEI GGTNACC 1 cut(s) 3212
PspFI CCCAGC 1 cut(s) 3639
PspGI CCWGG 8 cut(s) 794, 1012, 1996, 2876, 2887, 2974, 3034, 3130
PspPI GGNCC 5 cut(s) 1673, 2418, 2513, 2603, 2885
PspPPI RGGWCCY 4 cut(s) 1673, 2418, 2513, 2603
PstI CTGCAG 3 cut(s) 550, 823, 3899
PstNI CAGNNNCTG 5 cut(s) 1028, 1428, 1549, 2806, 2993
PsuI RGATCY 9 cut(s) 649, 1024, 1169, 1883, 2122, 3012, 3256, 3421, 3629
PsyI GACNNNGTC 1 cut(s) 3746
PvuI CGATCG 1 cut(s) 3286
PvuII CAGCTG 1 cut(s) 1247
RsaI GTAC 4 cut(s) 493, 503, 1087, 3556
RsaNI GTAC 4 cut(s) 492, 502, 1086, 3555
RseI CAYNNNNRTG 1 cut(s) 200
SapI GCTCTTC 2 cut(s) 2147, 3612
SatI GCNGC 9 cut(s) 840, 1245, 1975, 2282, 2361, 2364, 3591, 3623, 3693
Sau96I GGNCC 5 cut(s) 1673, 2418, 2513, 2603, 2885
SchI GAGTC 3 cut(s) 968, 1511, 3848
SduI GDGCHC 7 cut(s) 1102, 1810, 2333, 3454, 3703, 3797, 3884
SexAI ACCWGGT 1 cut(s) 3034
SfcI CTRYAG 4 cut(s) 546, 819, 1860, 3895
SfuI TTCGAA 1 cut(s) 3437
SinI GGWCC 4 cut(s) 1673, 2418, 2513, 2603
SmiMI CAYNNNNRTG 1 cut(s) 200
SmlI CTYRAG 3 cut(s) 1809, 1931, 2691
SmoI CTYRAG 3 cut(s) 1809, 1931, 2691
SpeI ACTAGT 2 cut(s) 517, 1852
SphI GCATGC 1 cut(s) 1710
SsiI CCGC 8 cut(s) 1778, 2460, 3591, 3625, 3718, 3890, 3946, 3984
SspI AATATT 1 cut(s) 967
StyI CCWWGG 1 cut(s) 2607
TaiI ACGT 3 cut(s) 451, 1930, 3556
TaqI TCGA 9 cut(s) 9, 1062, 1816, 2273, 2290, 3282, 3376, 3437, 3532
TatI WGTACW 1 cut(s) 501
TauI GCSGC 1 cut(s) 3593
TseFI GTSAC 5 cut(s) 241, 377, 431, 3212, 3840
TseI GCWGC 8 cut(s) 839, 1244, 1974, 2281, 2360, 2363, 3622, 3692
Tsp45I GTSAC 5 cut(s) 241, 377, 431, 3212, 3840
TspGWI ACGGA 3 cut(s) 2989, 3574, 3899
Tth111I GACNNNGTC 1 cut(s) 3746
VneI GTGCAC 2 cut(s) 1098, 3699
VpaK11BI GGWCC 4 cut(s) 1673, 2418, 2513, 2603
VspI ATTAAT 1 cut(s) 3372
XagI CCTNNNNNAGG 3 cut(s) 2579, 2892, 3116
XapI RAATTY 9 cut(s) 104, 230, 273, 613, 682, 1056, 1293, 2242, 3563
XbaI TCTAGA 2 cut(s) 3151, 3752
XceI RCATGY 3 cut(s) 1139, 1710, 2857
XcmI CCANNNNNNNNNTGG 3 cut(s) 875, 1315, 2374
XmiI GTMKAC 2 cut(s) 60, 1386
XmnI GAANNNNTTC 3 cut(s) 309, 1228, 2242
Zsp2I ATGCAT 3 cut(s) 1483, 1738, 2859
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.