MD01G1234000.v1.1

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0004502
Physical Location & Seq
Forward (+)
32171471 .. 32180233
8763 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1234000.v1.1.491

Sequence Viewer

Length: 4035 bp
ATGGGTGTCGAGAACTACCACGTGATTGAGCTTGTAGGCGAAGGTTCCTTTGGGAAGGTATACAAGGGAAGGCGAAAGAACACGGGCCAGACTGTTGCGATGAAATTCATTATGAAGCACGGCAAAAGTGACAAAGATATCCACAATTTAAGACAAGAAATTGAGATCCTAAGAAAGTTGAAGCACGAAAACATCATTGAAATGCTTGATTCATTTGAAAGCCCACAAGAGTTTTGTGTTGTTACAGAATTTGCACAAGGTGAACTATTTGAAATTCTTGAGGATGATAAGTGCCTTCCTGAAGAACAAGTTCAAGCTATTGCAAAGCAGCTGGTGAGAGCATTGCATTATCTGCATTCCAACCGTATCATCCATCGTGACATGAAACCACAAAACATTCTCATTGGTGCTGGTTCTATTGTTAAGCTCTGTGATTTTGGTTTTGCACGTGCAATGTCCACAAACACTGTTGTTTTGCGATCCATTAAAGGTACTCCTCTGTACATGGCGCCTGAACTAGTACGGGAACAACCCTACAACCACACTGCAGATCTGTGGTCTCTAGGAGTAATATTATATGAATTATTTGTAGGCCAGCCTCCATTTTATACAAACTCTGTATATGCACTCATCAGGCACATTGTTAAGGATCCAGTTAAATATCCTGACAACATAAGTCCAAGTTTTAAAAACTTTCTTAAGGGATTGCTTAATAAGGTGCCTCAAAATCGATTGACTTGGCCAGCTCTTCTGGAACACCCATTTGTCAAAGAGACGCCTCCCGAACTGGAGGCTAGGGAAATGCGTTCTGCAACTGCTGCAGAAAGGGGATGTGTTGCGGCATGGAGGAGTGAAGGAAACAAAGTTCAAACATCAGATGGTCTGGCTGTTTCATCTCCTGTCCACTCTTCAGCTTCTTCAGAGAACAACAGTGGGATAAGTTTTCAGAATGATGCTCAAGCAAATACTCCTGAATCCACAACAGTCAATTCTTCCCCAAATGAGTTCCCAGGATTAACAAATCCTAGTGAAGTCAAACAGTCAGGTTGCCAAATTTTGGACAGATTGGAAAATAACTCTCGTACAGTCAAGGGTGCACAAATAATAAGTCAAGATAATGAAGCATTAGCACATGTTTTGTTGCCACTAAAAAGATGCTCCAATGGACCCCCAGATTCATGCAGGGATGAAGATATTCTTAATTCAAAACAGTCTTTGAGAATTCTTTCAAACTTAGTTGCAGCTGGTGCCTTCCATTCCAGCGAGCTGTTTGATGAAATAATACATGAATTTCTTGCTTACTCTGCTATCATTGTGAGCATGAAATCTTCTGAAGTAAATGAGTTAAAAGCGAAGAGTTTCTCAATAATTAAAATTTTGGTTGACAATGCTGGAAGCAGCATTGGTGGCTCATATTTCAGGCACTGGGTTGTCTTATCAGATATTTTTTCACAGGTTGTTGGTTGTTGTGAAGATGCATCTGGAAGAGTTTTGTATGAGTCCATTGCTTGCATTACTGTCATGTTAAGAAGAGTCACACAGGGCCTTAAAGCTTTTTCCTCAACTTCAGTTCCTGAGGCAGCTTCTGATCCTAATGAAGCACTGAAACAGATTCTGGATCACACTAAAACATCTGGTTTAGTGGACCAACTTTGTCTCTGCTTAGTAACTGCAGGGTCAAGTCTTATTTCAGGCTCTTCAAATATGCTGTGTGCGGCTTGTGAGGCATGTACGGCAATTTGGTTTCTGATAGATGCATCAGAAAATCTTTCTATGAAAAGAAATGCCTATTTATTCCCCCTAAACACTTTGCGCCGGCCTTCACCCCAACTTGACATTAGGGATCAAGATCAAGGTTCTTTGATTGGGACAGAAGCAGCAAAACTTGTTGCTGTAATTACGAGAGCATTCCTTAGATCAAAAGCAGTACAAGTTGCTATCCATTATTGCCTTCATCAACGACTTGAAGCTTCGCTATATGCTGGCATTCAGTTATTGTTGAGGTGCTGCTTGCATAATGGAATTGTTCCTGGTGTTCTCTGTGGCCTGCCTAGTTCCCTACCTGTAACTACTGTTGTTAGTGGTGGAGGTGATGGAACCATTATTTCAGAGATATTCTCTTTGTTGTCTTTGTGCATTTCATCTCAAAATAAAGATCCCCAAGCAATTGAGACAGCCACCTTCAAGAACAAATTAACCAATCCCACTACCTTGGTTCTGCATTCATGCCTCATCCTTGCAACAGTTGCACAATGTTTGAAGGCAACTGGAAGAAATTCTGCACTATTTATGCTAACAACATCTCCAAAGAAGCAGCTTTCTCGACTTTCTGTTCTTGCTCATCATTTTTCTTCTGATGAGAGCAAAAACGCCTCCATTCAAGCTCATGCTGCATCGGCCATGCTGGCACTGGCTTCCATTTTGTACCTTGAACCTGGCAGTTCTGTTGAGTCTTCTATCCTCGAGGTAGCAGTGCCTTTGATTCCTAGAAGTGCCACACTATGTGAATACCTGAAGCTTTCATCAGGCAATGGAATTGGATTGCATTCGAATGGTCCTAGTGGTACTCTCTCATATTGGCATGGTCTAAGGGATGGATGTGTTGGCTTGCTGGAGTCTAGACTGAGGTGGGGAGGACCCTTAGCTGTTAAACAGCTGTGTGCAAGCAATATGCCTCTGCTTCTAGTGGATTTGTTAGCCAACAACCAACCAGAAGTTGGTAGCAACAATGATCAAGTTGGGCTTACCCCTATAGGAGTCATATGGACAATATCGTCAATATGTCACTGCCTTTCAGGTGGAGCCTTAACTTTTCGTCAGATCCTGCTTAGATGTAATCATATCAAGCTCATCTCCGACTTGATAGCTAATATGCATCTCAAGCTTGTAAAATCTTGGGTTGGGCCCGGTGGAGGGAAAGATGGTGTAAGAGATATAACAAATGCAGTAATTGACGTCTTGGCATTTCCTTTTGTGGCTGTACAGAATGCTTCAGGATTGCCATCAGCCACTGCTTCAGTTAATAGCGGGGCCCTTCTCAACCTGGGTTCACCAGGGGGTAGAGTTTGCATGAAAGACAAAGACATGGTGAAAGTAATTGAAGAAGACCTTGGGAAGTTTATTAAAAACCTTTTGGAGGTAGGAGTGCCAGGCATCGTTCTTTGGTGTTTAGAACACTTGGATTTAAAGGATATGGGAAGACCAGTGGCCTTTCTTGCTAAAATGATTGGTTACCGACCATTGGCAGTTCAAATTGTGGGTAAAGGCTTGTTGGATCCTAAAACGATGAGAAGATTGCTGGACTGTTCTAGTCCAAGAGAGGTGATGCTGGATGTTCTAATGATTGTTTCTGATTTGGCTCGCATGGACAAGGGATTCTACGAATACATTAATGGAGCATCTGTATTGGAGCTCTTCAAGGAATTTCTCACCCATGAAAATCCTAATATGCGCTCCAAGGCTTGTAGTGCTCTAGGAAATATGTGCCGGCACAGCTCCTACTTTTATAGTTCCCTGGCAAGGAATCAAATCATTGATCTCCTTATTGATCGATGTTCTGATCCTGATAAACGGACACGAAAATTTGCTTGCTTTGCTATTGGGAATGCTGCCTACCATGACAAGATGTTATATGAAGAGCTACGAAGATCCATACCCTCTCTTGTGAATTTGTTGGTTTCGTCCGAAGAAGACAAGACTAAAGCAAATGCAGCAGGTGCACTAAGCAATCTTGTCCGCCACTCCGATCAGCTTTGTGAAGACATTGTATCCAAAGGAGCCATGCAGTCTTTACTGAAGTTGGTGGCTGATTGTTCGGTGGTAGCACTGAACCCGCCGAGTAGAAAAGACTCAGCAAACGAGTCACCTCTTAAAATAGCTCTGATTGCATTGGCAAATATGTGTTCGCATCAGCCCTGCAGACAATTCCTCGGTTCATCACCGTTGTTCACTGTGATCGGACGCCTTCGGCAGTCTCCGGAATCAACAATTGCCAATCACGCCTCGCTGATATTCGCCAAAGTTGCTGATTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000166 GO:0000278 GO:0000280 GO:0000281 GO:0000910 GO:0000911 GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005856 GO:0005874 GO:0005881 GO:0006355 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006935 GO:0006996 GO:0007049 GO:0007112 GO:0007140 GO:0007154 GO:0007163 GO:0007165 GO:0007166 GO:0007224 GO:0007227 GO:0007228 GO:0007275 GO:0007276 GO:0007349 GO:0007584 GO:0008064 GO:0008092 GO:0008134 GO:0008144 GO:0008150 GO:0008152 GO:0008589 GO:0009524 GO:0009553 GO:0009558 GO:0009561 GO:0009605 GO:0009653 GO:0009791 GO:0009889 GO:0009966 GO:0009967 GO:0009987 GO:0009991 GO:0010033 GO:0010243 GO:0010468 GO:0010556 GO:0010639 GO:0010646 GO:0010647 GO:0014070 GO:0015630 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017076 GO:0019219 GO:0019222 GO:0019538 GO:0019894 GO:0019953 GO:0022402 GO:0022412 GO:0022414 GO:0022607 GO:0023051 GO:0023052 GO:0023056 GO:0030010 GO:0030030 GO:0030031 GO:0030554 GO:0030832 GO:0030833 GO:0030837 GO:0031156 GO:0031272 GO:0031323 GO:0031326 GO:0031333 GO:0031344 GO:0031667 GO:0032101 GO:0032104 GO:0032107 GO:0032271 GO:0032272 GO:0032501 GO:0032502 GO:0032504 GO:0032506 GO:0032535 GO:0032553 GO:0032555 GO:0032559 GO:0032956 GO:0032970 GO:0033043 GO:0033206 GO:0033273 GO:0035556 GO:0035639 GO:0036094 GO:0036211 GO:0040011 GO:0042221 GO:0042330 GO:0042493 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043254 GO:0043326 GO:0043327 GO:0043412 GO:0043900 GO:0044085 GO:0044087 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0044703 GO:0044782 GO:0045880 GO:0048229 GO:0048232 GO:0048285 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048609 GO:0048646 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051090 GO:0051091 GO:0051128 GO:0051129 GO:0051171 GO:0051252 GO:0051301 GO:0051321 GO:0051493 GO:0051494 GO:0051593 GO:0051704 GO:0051716 GO:0060176 GO:0060255 GO:0060271 GO:0060491 GO:0061640 GO:0065007 GO:0065008 GO:0065009 GO:0070925 GO:0071704 GO:0071840 GO:0080090 GO:0080134 GO:0080135 GO:0090066 GO:0097159 GO:0097367 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0110053 GO:0120031 GO:0120032 GO:0120035 GO:0120036 GO:0140013 GO:0140096 GO:1901265 GO:1901363 GO:1901564 GO:1901698 GO:1901700 GO:1902410 GO:1902903 GO:1902904 GO:1903046 GO:1903047 GO:1903506 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04341 map04341
Pfam Domains
Protein Families
Arm HEAT HEAT_2 Pkinase

Protein Analysis

1345

Amino Acids

146.4

Weight (kDa)

7.77

Isoelectric Point (pI)

47.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 6 - 256 1.6e-79 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 8 - 251 5.6e-47 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 3704
AasI GACNNNNNNGTC 1 cut(s) 2773
AatII GACGTC 1 cut(s) 2958
AbsI CCTCGAGG 1 cut(s) 2462
Acc36I ACCTGC 1 cut(s) 3704
AccB1I GGYRCC 3 cut(s) 508, 718, 1247
AccB7I CCANNNNNTGG 2 cut(s) 1057, 2717
AccI GTMKAC 1 cut(s) 60
AccIII TCCGGA 1 cut(s) 3976
AciI CCGC 5 cut(s) 839, 1715, 3027, 3736, 3833
AcoI YGGCCR 2 cut(s) 740, 2397
AcuI CTGAAG 9 cut(s) 321, 894, 903, 1353, 1551, 2534, 2976, 3000, 3815
AcvI CACGTG 2 cut(s) 22, 449
AcyI GRCGYC 4 cut(s) 509, 776, 2955, 3961
AdeI CACNNNGTG 2 cut(s) 260, 2504
AfaI GTAC 9 cut(s) 493, 503, 522, 1084, 1732, 1929, 2426, 2566, 2982
AfiI CCNNNNNNNGG 7 cut(s) 1057, 2717, 2912, 2913, 3136, 3241, 3519
AflII CTTAAG 1 cut(s) 698
AflIII ACRYGT 1 cut(s) 1132
AhlI ACTAGT 1 cut(s) 517
AjnI CCWGG 7 cut(s) 1009, 2029, 2434, 3042, 3052, 3148, 3513
AjuI GAANNNNNNNTTGG 4 cut(s) 33, 65, 3093, 3125
AloI GAACNNNNNNTCC 2 cut(s) 849, 881
Alw21I GWGCWC 4 cut(s) 1099, 3414, 3472, 3721
Alw26I GTCTC 5 cut(s) 564, 767, 1661, 2165, 3978
Alw44I GTGCAC 2 cut(s) 1095, 3717
AlwNI CAGNNNCTG 6 cut(s) 1425, 1574, 1586, 1615, 2824, 3011
Ama87I CYCGRG 1 cut(s) 2462
Aor13HI TCCGGA 1 cut(s) 3976
ApaI GGGCCC 2 cut(s) 2907, 3034
ApaLI GTGCAC 2 cut(s) 1095, 3717
ArsI GACNNNNNNTTYG 2 cut(s) 1093, 1125
AseI ATTAAT 1 cut(s) 3390
Asp700I GAANNNNTTC 5 cut(s) 309, 1194, 1225, 1358, 2275
AspLEI GCGC 3 cut(s) 511, 1815, 3453
AsuC2I CCSGG 1 cut(s) 2907
AsuII TTCGAA 1 cut(s) 2549
AvaI CYCGRG 1 cut(s) 2462
AvaII GGWCC 4 cut(s) 1166, 1645, 2555, 2636
AxyI CCTNAGG 1 cut(s) 1575
BaeGI GKGCMC 4 cut(s) 1099, 2907, 3034, 3721
BalI TGGCCA 1 cut(s) 742
BamHI GGATCC 2 cut(s) 649, 3274
BanI GGYRCC 3 cut(s) 508, 718, 1247
BanII GRGCYC 3 cut(s) 2907, 3034, 3414
BbrPI CACGTG 2 cut(s) 22, 449
BbsI GAAGAC 5 cut(s) 2445, 3111, 3205, 3696, 3765
Bbv12I GWGCWC 4 cut(s) 1099, 3414, 3472, 3721
BccI CCATC 6 cut(s) 381, 872, 2087, 2588, 2915, 3010
BceAI ACGGC 2 cut(s) 136, 1749
BcgI CGANNNNNNTGC 2 cut(s) 2303, 2337
BciT130I CCWGG 7 cut(s) 1011, 2031, 2436, 3044, 3054, 3150, 3515
BciVI GTATCC 1 cut(s) 3778
BclI TGATCA 1 cut(s) 2731
BcnI CCSGG 1 cut(s) 2907
BcoDI GTCTC 5 cut(s) 564, 767, 1661, 2165, 3978
BcuI ACTAGT 1 cut(s) 517
BfmI CTRYAG 5 cut(s) 546, 819, 1671, 2751, 3916
BfoI RGCGCY 1 cut(s) 512
BfrI CTTAAG 1 cut(s) 698
BfuAI ACCTGC 1 cut(s) 3704
BfuI GTATCC 1 cut(s) 3778
BglI GCCNNNNNGGC 1 cut(s) 2405
BglII AGATCT 1 cut(s) 550
Bme1390I CCNGG 8 cut(s) 1011, 2031, 2436, 2907, 3044, 3054, 3150, 3515
Bme18I GGWCC 4 cut(s) 1166, 1645, 2555, 2636
BmeT110I CYCGRG 1 cut(s) 2462
BmrFI CCNGG 8 cut(s) 1011, 2031, 2436, 2907, 3044, 3054, 3150, 3515
BmrI ACTGGG 1 cut(s) 1435
BmuI ACTGGG 1 cut(s) 1435
BpiI GAAGAC 5 cut(s) 2445, 3111, 3205, 3696, 3765
BplI GAGNNNNNCTC 2 cut(s) 2102, 2134
BpmI CTGGAG 2 cut(s) 809, 2633
Bpu10I CCTNAGC 1 cut(s) 2641
Bpu14I TTCGAA 1 cut(s) 2549
BpuEI CTTGAG 3 cut(s) 299, 942, 2864
BpuMI CCSGG 1 cut(s) 2907
Bsa29I ATCGAT 2 cut(s) 730, 3550
BsaAI YACGTR 2 cut(s) 22, 449
BsaBI GATNNNNATC 2 cut(s) 1848, 3001
BsaHI GRCGYC 4 cut(s) 509, 776, 2955, 3961
BsaI GGTCTC 1 cut(s) 564
BsaJI CCNNGG 8 cut(s) 1009, 2211, 3043, 3053, 3109, 3456, 3513, 3928
BsaWI WCCGGW 1 cut(s) 3976
BsaXI ACNNNNNCTCC 4 cut(s) 2287, 2317, 3387, 3417
Bsc4I CCNNNNNNNGG 7 cut(s) 1057, 2717, 2912, 2913, 3136, 3241, 3519
Bse118I RCCGGY 2 cut(s) 1815, 3486
Bse1I ACTGG 6 cut(s) 653, 792, 1430, 2272, 2415, 3203
Bse21I CCTNAGG 1 cut(s) 1575
Bse3DI GCAATG 4 cut(s) 341, 459, 1503, 2536
Bse8I GATNNNNATC 2 cut(s) 1848, 3001
BseAI TCCGGA 1 cut(s) 3976
BseBI CCWGG 7 cut(s) 1011, 2031, 2436, 3044, 3054, 3150, 3515
BseCI ATCGAT 2 cut(s) 730, 3550
BseDI CCNNGG 8 cut(s) 1009, 2211, 3043, 3053, 3109, 3456, 3513, 3928
BseGI GGATG 8 cut(s) 289, 369, 836, 1192, 2232, 2599, 2603, 3337
BseJI GATNNNNATC 2 cut(s) 1848, 3001
BseLI CCNNNNNNNGG 7 cut(s) 1057, 2717, 2912, 2913, 3136, 3241, 3519
BseMI GCAATG 4 cut(s) 341, 459, 1503, 2536
BseMII CTCAG 3 cut(s) 1566, 2615, 3864
BseNI ACTGG 6 cut(s) 653, 792, 1430, 2272, 2415, 3203
BseRI GAGGAG 2 cut(s) 486, 862
BseSI GKGCMC 4 cut(s) 1099, 2907, 3034, 3721
BsgI GTGCAG 1 cut(s) 2265
BshNI GGYRCC 3 cut(s) 508, 718, 1247
BshVI ATCGAT 2 cut(s) 730, 3550
BsiHKAI GWGCWC 4 cut(s) 1099, 3414, 3472, 3721
BsiHKCI CYCGRG 1 cut(s) 2462
BsiSI CCGG 4 cut(s) 1816, 2907, 3487, 3977
BslFI GGGAC 1 cut(s) 1882
BslI CCNNNNNNNGG 7 cut(s) 1057, 2717, 2912, 2913, 3136, 3241, 3519
BsmAI GTCTC 5 cut(s) 564, 767, 1661, 2165, 3978
BsmBI CGTCTC 1 cut(s) 767
BsmFI GGGAC 1 cut(s) 1882
BsmI GAATGC 7 cut(s) 355, 1907, 1986, 2221, 2545, 2992, 3610
Bso31I GGTCTC 1 cut(s) 564
BsoBI CYCGRG 1 cut(s) 2462
Bsp119I TTCGAA 1 cut(s) 2549
Bsp120I GGGCCC 2 cut(s) 2903, 3030
Bsp1286I GDGCHC 6 cut(s) 1099, 2907, 3034, 3414, 3472, 3721
Bsp13I TCCGGA 1 cut(s) 3976
Bsp1407I TGTACA 2 cut(s) 501, 2980
BspACI CCGC 5 cut(s) 839, 1715, 3027, 3736, 3833
BspCNI CTCAG 3 cut(s) 1567, 2616, 3863
BspDI ATCGAT 2 cut(s) 730, 3550
BspEI TCCGGA 1 cut(s) 3976
BspMAI CTGCAG 4 cut(s) 550, 823, 1675, 3920
BspMI ACCTGC 1 cut(s) 3704
BspQI GCTCTTC 4 cut(s) 753, 1702, 3419, 3630
BspT104I TTCGAA 1 cut(s) 2549
BspT107I GGYRCC 3 cut(s) 508, 718, 1247
BspTI CTTAAG 1 cut(s) 698
BspTNI GGTCTC 1 cut(s) 564
BsrDI GCAATG 4 cut(s) 341, 459, 1503, 2536
BsrFI RCCGGY 2 cut(s) 1815, 3486
BsrGI TGTACA 2 cut(s) 501, 2980
BsrI ACTGG 6 cut(s) 653, 792, 1430, 2272, 2415, 3203
BssAI RCCGGY 2 cut(s) 1815, 3486
BssECI CCNNGG 8 cut(s) 1009, 2211, 3043, 3053, 3109, 3456, 3513, 3928
BssNAI GTATAC 1 cut(s) 61
BssNI GRCGYC 4 cut(s) 509, 776, 2955, 3961
BssT1I CCWWGG 3 cut(s) 2211, 3109, 3456
Bst1107I GTATAC 1 cut(s) 61
Bst2UI CCWGG 7 cut(s) 1011, 2031, 2436, 3044, 3054, 3150, 3515
Bst6I CTCTTC 8 cut(s) 753, 913, 1349, 1480, 1525, 1702, 3419, 3630
BstACI GRCGYC 4 cut(s) 509, 776, 2955, 3961
BstAFI CTTAAG 1 cut(s) 698
BstAPI GCANNNNNTGC 5 cut(s) 352, 818, 1247, 2246, 3716
BstAUI TGTACA 2 cut(s) 501, 2980
BstBAI YACGTR 2 cut(s) 22, 449
BstBI TTCGAA 1 cut(s) 2549
BstEII GGTNACC 1 cut(s) 3230
BstENI CCTNNNNNAGG 1 cut(s) 3134
BstF5I GGATG 8 cut(s) 289, 369, 836, 1192, 2232, 2599, 2603, 3337
BstH2I RGCGCY 1 cut(s) 512
BstHHI GCGC 3 cut(s) 511, 1815, 3453
BstMAI GTCTC 5 cut(s) 564, 767, 1661, 2165, 3978
BstNI CCWGG 7 cut(s) 1011, 2031, 2436, 3044, 3054, 3150, 3515
BstNSI RCATGY 2 cut(s) 1136, 1731
BstPI GGTNACC 1 cut(s) 3230
BstSCI CCNGG 8 cut(s) 1009, 2029, 2434, 2905, 3042, 3052, 3148, 3513
BstSFI CTRYAG 5 cut(s) 546, 819, 1671, 2751, 3916
BstSLI GKGCMC 4 cut(s) 1099, 2907, 3034, 3721
BstV2I GAAGAC 5 cut(s) 2445, 3111, 3205, 3696, 3765
BstX2I RGATCY 7 cut(s) 165, 550, 649, 2155, 2820, 3274, 3647
BstXI CCANNNNNNTGG 1 cut(s) 2212
BstYI RGATCY 7 cut(s) 165, 550, 649, 2155, 2820, 3274, 3647
BstZ17I GTATAC 1 cut(s) 61
Bsu15I ATCGAT 2 cut(s) 730, 3550
Bsu36I CCTNAGG 1 cut(s) 1575
BsuI GTATCC 1 cut(s) 3778
BsuTUI ATCGAT 2 cut(s) 730, 3550
BtgZI GCGATG 1 cut(s) 113
BtsCI GGATG 8 cut(s) 289, 369, 836, 1192, 2232, 2599, 2603, 3337
BtsI GCAGTG 4 cut(s) 543, 2478, 2785, 3009
BveI ACCTGC 1 cut(s) 3704
CaiI CAGNNNCTG 6 cut(s) 1425, 1574, 1586, 1615, 2824, 3011
CfoI GCGC 3 cut(s) 511, 1815, 3453
Cfr10I RCCGGY 2 cut(s) 1815, 3486
ClaI ATCGAT 2 cut(s) 730, 3550
CseI GACGC 2 cut(s) 784, 3969
Csp6I GTAC 9 cut(s) 492, 502, 521, 1083, 1731, 1928, 2425, 2565, 2981
CviQI GTAC 9 cut(s) 492, 502, 521, 1083, 1731, 1928, 2425, 2565, 2981
DinI GGCGCC 1 cut(s) 510
DraI TTTAAA 2 cut(s) 688, 3186
DraIII CACNNNGTG 2 cut(s) 260, 2504
DrdI GACNNNNNNGTC 1 cut(s) 2773
DseDI GACNNNNNNGTC 1 cut(s) 2773
EaeI YGGCCR 2 cut(s) 740, 2397
Eam1104I CTCTTC 8 cut(s) 753, 913, 1349, 1480, 1525, 1702, 3419, 3630
EarI CTCTTC 8 cut(s) 753, 913, 1349, 1480, 1525, 1702, 3419, 3630
EciI GGCGGA 1 cut(s) 3725
Ecl136II GAGCTC 1 cut(s) 3412
Eco130I CCWWGG 3 cut(s) 2211, 3109, 3456
Eco24I GRGCYC 3 cut(s) 2907, 3034, 3414
Eco31I GGTCTC 1 cut(s) 564
Eco32I GATATC 1 cut(s) 139
Eco47I GGWCC 4 cut(s) 1166, 1645, 2555, 2636
Eco53kI GAGCTC 1 cut(s) 3412
Eco57I CTGAAG 9 cut(s) 321, 894, 903, 1353, 1551, 2534, 2976, 3000, 3815
Eco72I CACGTG 2 cut(s) 22, 449
Eco81I CCTNAGG 1 cut(s) 1575
Eco88I CYCGRG 1 cut(s) 2462
Eco91I GGTNACC 1 cut(s) 3230
EcoICRI GAGCTC 1 cut(s) 3412
EcoNI CCTNNNNNAGG 1 cut(s) 3134
EcoO109I RGGNCCY 4 cut(s) 1543, 2636, 3030, 3031
EcoO65I GGTNACC 1 cut(s) 3230
EcoRI GAATTC 1 cut(s) 1221
EcoRII CCWGG 7 cut(s) 1009, 2029, 2434, 3042, 3052, 3148, 3513
EcoRV GATATC 1 cut(s) 139
EcoT14I CCWWGG 3 cut(s) 2211, 3109, 3456
EcoT22I ATGCAT 3 cut(s) 1480, 1759, 2877
EcoT38I GRGCYC 3 cut(s) 2907, 3034, 3414
EgeI GGCGCC 1 cut(s) 510
EheI GGCGCC 1 cut(s) 510
ErhI CCWWGG 3 cut(s) 2211, 3109, 3456
Esp3I CGTCTC 1 cut(s) 767
FalI AAGNNNNNCTT 6 cut(s) 1182, 1214, 2727, 2759, 3093, 3125
FaqI GGGAC 1 cut(s) 1882
FauI CCCGC 2 cut(s) 3020, 3840
FauNDI CATATG 1 cut(s) 2762
FbaI TGATCA 1 cut(s) 2731
FblI GTMKAC 1 cut(s) 60
FokI GGATG 8 cut(s) 296, 356, 843, 1199, 2219, 2606, 2610, 3344
FriOI GRGCYC 3 cut(s) 2907, 3034, 3414
GlaI GCGC 3 cut(s) 510, 1814, 3452
GsuI CTGGAG 2 cut(s) 809, 2633
HaeII RGCGCY 1 cut(s) 512
HapII CCGG 4 cut(s) 1816, 2907, 3487, 3977
HgaI GACGC 2 cut(s) 784, 3969
HhaI GCGC 3 cut(s) 511, 1815, 3453
Hin1I GRCGYC 4 cut(s) 509, 776, 2955, 3961
Hin6I GCGC 3 cut(s) 509, 1813, 3451
HinP1I GCGC 3 cut(s) 509, 1813, 3451
HincII GTYRAC 1 cut(s) 1384
HindII GTYRAC 1 cut(s) 1384
HindIII AAGCTT 4 cut(s) 1551, 1968, 2516, 2882
HpaII CCGG 4 cut(s) 1816, 2907, 3487, 3977
HpyCH4IV ACGT 3 cut(s) 21, 448, 2955
HpySE526I ACGT 3 cut(s) 21, 448, 2955
Hsp92I GRCGYC 4 cut(s) 509, 776, 2955, 3961
HspAI GCGC 3 cut(s) 509, 1813, 3451
KasI GGCGCC 1 cut(s) 508
Kpn2I TCCGGA 1 cut(s) 3976
KroI GCCGGC 2 cut(s) 1815, 3486
KroNI GCCGGC 2 cut(s) 1817, 3488
Ksp22I TGATCA 1 cut(s) 2731
LguI GCTCTTC 4 cut(s) 753, 1702, 3419, 3630
LmnI GCTCC 7 cut(s) 1163, 2801, 3395, 3409, 3458, 3500, 3776
MaeII ACGT 3 cut(s) 21, 448, 2955
MaeIII GTNAC 9 cut(s) 128, 241, 377, 1534, 1666, 2065, 2783, 3230, 3861
MfeI CAATTG 2 cut(s) 2166, 3987
MflI RGATCY 7 cut(s) 165, 550, 649, 2155, 2820, 3274, 3647
MhlI GDGCHC 6 cut(s) 1099, 2907, 3034, 3414, 3472, 3721
MlsI TGGCCA 1 cut(s) 742
MluNI TGGCCA 1 cut(s) 742
Mly113I GGCGCC 1 cut(s) 509
MlyI GAGTC 7 cut(s) 1508, 1542, 2459, 2624, 2766, 3842, 3869
MmeI TCCRAC 3 cut(s) 384, 2880, 3252
Mox20I TGGCCA 1 cut(s) 742
Mph1103I ATGCAT 3 cut(s) 1480, 1759, 2877
MroI TCCGGA 1 cut(s) 3976
MroNI GCCGGC 2 cut(s) 1815, 3486
MroXI GAANNNNTTC 5 cut(s) 309, 1194, 1225, 1358, 2275
MscI TGGCCA 1 cut(s) 742
MslI CAYNNNNRTG 2 cut(s) 200, 2550
Msp20I TGGCCA 1 cut(s) 742
MspA1I CMGCKG 3 cut(s) 331, 1244, 2656
MspCI CTTAAG 1 cut(s) 698
MspI CCGG 4 cut(s) 1816, 2907, 3487, 3977
MspR9I CCNGG 8 cut(s) 1011, 2031, 2436, 2907, 3044, 3054, 3150, 3515
MunI CAATTG 2 cut(s) 2166, 3987
Mva1269I GAATGC 7 cut(s) 355, 1907, 1986, 2221, 2545, 2992, 3610
MvaI CCWGG 7 cut(s) 1011, 2031, 2436, 3044, 3054, 3150, 3515
NaeI GCCGGC 2 cut(s) 1817, 3488
NarI GGCGCC 1 cut(s) 509
NciI CCSGG 1 cut(s) 2907
NdeI CATATG 1 cut(s) 2762
NgoMIV GCCGGC 2 cut(s) 1815, 3486
NmeAIII GCCGAG 1 cut(s) 3861
NmuCI GTSAC 5 cut(s) 128, 377, 1534, 2783, 3861
NsiI ATGCAT 3 cut(s) 1480, 1759, 2877
NspI RCATGY 2 cut(s) 1136, 1731
NspV TTCGAA 1 cut(s) 2549
PaeR7I CTCGAG 1 cut(s) 2462
PaqCI CACCTGC 1 cut(s) 3704
PciI ACATGT 1 cut(s) 1132
PciSI GCTCTTC 4 cut(s) 753, 1702, 3419, 3630
PctI GAATGC 7 cut(s) 355, 1907, 1986, 2221, 2545, 2992, 3610
PdiI GCCGGC 2 cut(s) 1817, 3488
PdmI GAANNNNTTC 5 cut(s) 309, 1194, 1225, 1358, 2275
PfeI GAWTC 9 cut(s) 209, 974, 1175, 1612, 2482, 3375, 3523, 3980, 4028
PflMI CCANNNNNTGG 2 cut(s) 1057, 2717
PleI GAGTC 7 cut(s) 1507, 1541, 2458, 2623, 2765, 3842, 3868
PluTI GGCGCC 1 cut(s) 512
PmaCI CACGTG 2 cut(s) 22, 449
PmlI CACGTG 2 cut(s) 22, 449
PpsI GAGTC 7 cut(s) 1507, 1541, 2458, 2623, 2765, 3842, 3868
Ppu21I YACGTR 2 cut(s) 22, 449
PpuMI RGGWCCY 1 cut(s) 2636
PscI ACATGT 1 cut(s) 1132
PshBI ATTAAT 1 cut(s) 3390
Psp124BI GAGCTC 1 cut(s) 3414
Psp5II RGGWCCY 1 cut(s) 2636
Psp6I CCWGG 7 cut(s) 1009, 2029, 2434, 3042, 3052, 3148, 3513
PspCI CACGTG 2 cut(s) 22, 449
PspEI GGTNACC 1 cut(s) 3230
PspGI CCWGG 7 cut(s) 1009, 2029, 2434, 3042, 3052, 3148, 3513
PspOMI GGGCCC 2 cut(s) 2903, 3030
PspPPI RGGWCCY 1 cut(s) 2636
PspXI VCTCGAGB 1 cut(s) 2462
PstI CTGCAG 4 cut(s) 550, 823, 1675, 3920
PstNI CAGNNNCTG 6 cut(s) 1425, 1574, 1586, 1615, 2824, 3011
PsuI RGATCY 7 cut(s) 165, 550, 649, 2155, 2820, 3274, 3647
PvuII CAGCTG 3 cut(s) 331, 1244, 2656
RsaI GTAC 9 cut(s) 493, 503, 522, 1084, 1732, 1929, 2426, 2566, 2982
RsaNI GTAC 9 cut(s) 492, 502, 521, 1083, 1731, 1928, 2425, 2565, 2981
RseI CAYNNNNRTG 2 cut(s) 200, 2550
SacI GAGCTC 1 cut(s) 3414
SapI GCTCTTC 4 cut(s) 753, 1702, 3419, 3630
SchI GAGTC 7 cut(s) 1508, 1542, 2459, 2624, 2766, 3842, 3869
ScrFI CCNGG 8 cut(s) 1011, 2031, 2436, 2907, 3044, 3054, 3150, 3515
SduI GDGCHC 6 cut(s) 1099, 2907, 3034, 3414, 3472, 3721
SfcI CTRYAG 5 cut(s) 546, 819, 1671, 2751, 3916
SfoI GGCGCC 1 cut(s) 510
Sfr274I CTCGAG 1 cut(s) 2462
SfuI TTCGAA 1 cut(s) 2549
SinI GGWCC 4 cut(s) 1166, 1645, 2555, 2636
SlaI CTCGAG 1 cut(s) 2462
SmiMI CAYNNNNRTG 2 cut(s) 200, 2550
SmlI CTYRAG 5 cut(s) 278, 698, 957, 2462, 2879
SmoI CTYRAG 5 cut(s) 278, 698, 957, 2462, 2879
SpeI ACTAGT 1 cut(s) 517
SsiI CCGC 5 cut(s) 839, 1715, 3027, 3736, 3833
SspDI GGCGCC 1 cut(s) 508
SspI AATATT 1 cut(s) 573
SstI GAGCTC 1 cut(s) 3414
StyD4I CCNGG 8 cut(s) 1009, 2029, 2434, 2905, 3042, 3052, 3148, 3513
StyI CCWWGG 3 cut(s) 2211, 3109, 3456
TaiI ACGT 3 cut(s) 24, 451, 2958
TaqI TCGA 6 cut(s) 9, 730, 2323, 2463, 2549, 3550
TatI WGTACW 3 cut(s) 501, 1927, 2980
TauI GCSGC 2 cut(s) 842, 1718
TfiI GAWTC 9 cut(s) 209, 974, 1175, 1612, 2482, 3375, 3523, 3980, 4028
TseFI GTSAC 5 cut(s) 128, 377, 1534, 2783, 3861
Tsp45I GTSAC 5 cut(s) 128, 377, 1534, 2783, 3861
TspGWI ACGGA 1 cut(s) 3586
Van91I CCANNNNNTGG 2 cut(s) 1057, 2717
Vha464I CTTAAG 1 cut(s) 698
VneI GTGCAC 2 cut(s) 1095, 3717
VpaK11BI GGWCC 4 cut(s) 1166, 1645, 2555, 2636
VspI ATTAAT 1 cut(s) 3390
XagI CCTNNNNNAGG 1 cut(s) 3134
XbaI TCTAGA 1 cut(s) 2618
XceI RCATGY 2 cut(s) 1136, 1731
XcmI CCANNNNNNNNNTGG 2 cut(s) 2407, 2714
XhoI CTCGAG 1 cut(s) 2462
XmiI GTMKAC 1 cut(s) 60
XmnI GAANNNNTTC 5 cut(s) 309, 1194, 1225, 1358, 2275
ZraI GACGTC 1 cut(s) 2956
Zsp2I ATGCAT 3 cut(s) 1480, 1759, 2877
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.