Rh1DG459300

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0004502
Physical Location & Seq
Forward (+)
66350643 .. 66359560
8918 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG459300.1

Sequence Viewer

Length: 3996 bp
ATGGGTGTCGAGAATTATCATGTAATTGAGCTTGTAGGTGAAGGTTCCTTTGGGAAGGTATACAAGGGAAGACGCAAGTTCACTGGGCAGACCGTTGCAATGAAATTTATTATGAAACATGGGAAAAGCGACAAGGATATCCACAATTTAAGACAAGAAATAGAGATTCTAAGGAAGCTAAAGCATGAAAATATCATTGAAATGCTTGATTCCTTTGAAAGCCCACAAGAATTCTGTGTTGTCACAGAGTTTGCACAAGGTGAACTATTTGAAATTCTTGAAGATGATAAGCACCTTCCAGAAGAACAAGTTCAAGCAATTGCTAAGCAATTGGTGAGAGCATTGCATTATCTCCATTCAAACCGTATCATCCATCGTGACATGAAACCACAAAACATTCTCATTGGTGCTGGTTCTATTGTTAAGCTCTGTGACTTTGGGTTTGCACGTGCAATGTCCACAAATACTGTTGTTTTGCGATCCATAAAAGGTACTCCCCTGTACATGGCTCCTGAACTAGTACGGGAACAACCTTACAACCACACTGCAGATTTATGGTCTCTCGGAGTTATATTATATGAATTATTTGTTGGCCAGCCTCCATTTTATACAAATTCAGTATATGCACTCATTCGGCATATTGTGAAGGATCCAGTTAAATACCCTGATAACATGAGTTCAAATTTTAAAAACTTTCTGAAGGGATTGCTCAATAAGATGCCTCAAAATCGTTTGACTTGGCCAGCTCTTTTGGAACACCCATTTGTTAAGGAGACAACTGAAGAACTGGAGGCTAGGGAAATGCGTTCTGCTACTTCTGCAGAAAGGGGGTGCGTTGCAGCATGGAGGGGTGAAGGAAACAAAATCCAAACATCAGGTGGCTTGGCTGTTTCTAGTCCTGCTTCTTCAGAGAACAACAGTGGAATAAGTTTCCAAAATGATGCTCAAACAAATATTCCAGACTCTAAAGCAGTCAATTCTTCCCCAAATGAGGAGTTTCCAGGGTTTGCAGATCCTGATAAAGTTAAACAGTCAGGTTGTCAAATTCTCGACAGACTGGAAAATAACTCTCGTACAGTTAAAGGTGCACAAATAATTGGTCAAGATAATGAAGCATTAGCACATGTTTTGCTACCACTAAAAAGATGCTCGCAAGGATCCCAAAACTCTAGCAGGGATCAAGATATTCTTACTTCAAACCAGTCTTTAAGAATCCTTTCAAACTTAGTTGCAGCTGGTGCCATCACTTCCAGTGGGCTACTTGATGAAATCATACATGAAATTCTTGTTTATACCACCTTCATTGTGGGCATCAAATCTTCTGAACTGAATGAGTTTAGAGCAAAGAGTTTCTCAGTAATTAAAGTTTTGGTAGACATTGCTGGACATGGCATTGGAGGCTCATATTTCAGGCACTGGGTTGCTTTAGCTGATATTTTTTCACAGGTTGTTGGTGGCAGTGAAGATGCATCTGGAAGAGTTTTGTTTGAGTCCATAGCTTGCATTACTGTCATGTTAACAAGAGTTAATCAGGGTCTGAAACAGGCTGTTTCTGATCCTAATGAAGCAGTGAAACAGATTTTGGATCATGCTAAAACATCTGGTTTAGTGGATCAGCTTTGTCTCTGCTTAGCTACTGCTGGAGCAAGTCTTATTTCAGGTCCTTCAAATATGTCGCGTTTAGCCTGTGAAGCATGCAAGGCTATTTGGTTGCTGGTAGATGCATCAGAGTTTTTTTCGACAAAAGGAAATGCCATTTCCTTTCCGCTAAACACTTTGCGCAGTTCATCAAGGGCTCAAGATCGAGGAGGTTCTCTGATTGGAACAGAATCAGCAAGACTAGTAGCTGTAGTTACTAGAGCATTAGTTAGATCTAAACCAGTTCAGGTTGCCATCCATTATTGCCTTCGTCAACGTCTTGAGGCTTCACTCTGTGCTGGTATTCAGCTATTGTTGAGGTGCTGCTTGCAGAGTGGAATTGTTCCTGGCATTCTTTGTGGCCTGCCTAGTTCTTTACCTGTAACTACTGTTGTCAGTGGTGGAGGTGATGGAACCATAATTTTTGAGATATTCTCTTTGCTGTCTGTGTGCATTTCATCTCAAAATAAAGATCCACAAGCAATTGAGACAACCAACTTGAAGTGCAAAATAACCGATCCCACCATGTTGGTTCTGCATTCCTGCCTCATCCTTGCATCAGTTGCACAATGTTTAAAGGCAACTGGAAGAAATTCTGCATTGTTTATGCTTACTACATCCTCGAAGAAGCAGCTTTCTCGACTTTCTGCCCTTGCTCATCACTTTTCTTCCGGTGAGAGCACAAATACTTCCTCTCAAACTCACAGTGCAGCAGCCATGTTGGCACTGGCATCTATTTTGTCCCTTGAATCTGGAGCTTCTGTTGGGTCCTCTGTCTTTGAGGTAGCAGTGCCTTTGATTCCTCAAACCGCGACACTTTGTGAATGCCTCAAACTTTCATCAAACAATGAGAGTGTATCGGGTCCTAATGGCACCAACGGTGTTCTCTCAAACTGGCATGGTCTTAGGGATGGATGTGTTGGCCTGCTGGAGGCCAGACTGAGATGGGGAGGGCCCTTGGCTGTTCAACAACTTTGTGCAAGCAATATCCCTCTGCTTCTAATCAATTTGTTAGCCAAAAACCAACAAAATGCTTCTCCTCAAGAAGTTGCCAGCACAAATGATCAAGTTGGGCTTTCCCCTATTGGAGTTGTATGGACAGTATCATCTATTTGTCAGTGCCTTTCTGGTGGAGCCTTAACCTTTCGTCAGATTCTGCTTAGAAGTGAGCATATCAAACTCCTCTCTGACTTGATATCTGACATGCATCTCAAACTTGTAAAGTCCTGGGTTGGGCCTGGTGGAGGAATGGATGGCGTAAGAGATATAACAAACGCAGTAATTGACCTCTTGGCATTTCCTTTTGTGGCTGTTCAGAATGCTCCTGGATTGCCATCAGCGACTGCCTCAGTGAACAGTGGGATCCTTCTGAACATGGGTTCACCAGGTGTGAAAGTTGGCGTGGAAGACAGAGACATGGTGAAGGTGATTGAGGAAGACATCGGAAAATATATTAAAATCCTTTTGGAGGTTGGAGTGCCTGGTATCATTCTTTGGTGTCTAGAACATTTGGAGTTAAAAGATTTGGGAAGACCTGTGGCCTTTCTGGCTAAAATGATTGGTCACCGACCATTGGCAGTTCAACTTGTGGGTAAAGGCTTGTTGGATCCTACTAGGATGAGAAGATTGCTCGATCGTTCAAGCCCAAAAGAAGTCATGCTGGATGTTCTGATGATTGTTTCTGATTTGGCACGCATGGACAAGGGATTCTATGAATACATTAATCGAGCTTCTCTGCTGGAGTTCTTCAAGGGATTTCTTACCCATGAAGATCCTAGTGTGCGTTCGAAGGCTTGCAGTGCTCTAGGCAATATGTGCCGGCACAGCTCCTACTTTTACAGTTCCCTAGCAAAGAATCAAATCATTGGTCTCCTTATTGATCGATGCTCTGACCCAGACAAACGTACACGGAAATTTGCTTGTTTTGCTATTGGCAATGCCGCTTACCATAATGATATGTTGTATGAAGAGCTGCGGAGATCTATCCCCCAGCTTGCCAATTTGTTGCTTTCATCTGAGGAAGACAAGACTAAAGCAAATGCAGCAGGTGCACTAAGCAATCTTATCCGCAACTCCAACAAACTTTGCGAAGACATTGTCTCTAGAGGAGCCATGCAGTCTTTGCTGAAGTTGGTGGCAGAGTGCTCAGTGTTGGCTCTGAACCCAAGTAGGAAAGATTCAGCGCAAGAGTCACCTCTCAAAATAGCTCTGTTTTCATTGGCAAAAATGTGCTCGCATCCGCCCTGCAGAGATTTCCTCCGTTCATCTGAGTTGTTCCCCGTGATCGGACGGCTCCGCCAATCCCCGGAATCAACAATTGCCAACTACGCCTCCGCCATCATCAACAAAGTTGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000166 GO:0000278 GO:0000280 GO:0000281 GO:0000910 GO:0000911 GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005856 GO:0005874 GO:0005881 GO:0006355 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006935 GO:0006996 GO:0007049 GO:0007112 GO:0007140 GO:0007154 GO:0007163 GO:0007165 GO:0007166 GO:0007224 GO:0007227 GO:0007228 GO:0007275 GO:0007276 GO:0007349 GO:0007584 GO:0008064 GO:0008092 GO:0008134 GO:0008144 GO:0008150 GO:0008152 GO:0008589 GO:0009524 GO:0009553 GO:0009558 GO:0009561 GO:0009605 GO:0009653 GO:0009791 GO:0009889 GO:0009966 GO:0009967 GO:0009987 GO:0009991 GO:0010033 GO:0010243 GO:0010468 GO:0010556 GO:0010639 GO:0010646 GO:0010647 GO:0014070 GO:0015630 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017076 GO:0019219 GO:0019222 GO:0019538 GO:0019894 GO:0019953 GO:0022402 GO:0022412 GO:0022414 GO:0022607 GO:0023051 GO:0023052 GO:0023056 GO:0030010 GO:0030030 GO:0030031 GO:0030554 GO:0030832 GO:0030833 GO:0030837 GO:0031156 GO:0031272 GO:0031323 GO:0031326 GO:0031333 GO:0031344 GO:0031667 GO:0032101 GO:0032104 GO:0032107 GO:0032271 GO:0032272 GO:0032501 GO:0032502 GO:0032504 GO:0032506 GO:0032535 GO:0032553 GO:0032555 GO:0032559 GO:0032956 GO:0032970 GO:0033043 GO:0033206 GO:0033273 GO:0035556 GO:0035639 GO:0036094 GO:0036211 GO:0040011 GO:0042221 GO:0042330 GO:0042493 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043254 GO:0043326 GO:0043327 GO:0043412 GO:0043900 GO:0044085 GO:0044087 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0044703 GO:0044782 GO:0045880 GO:0048229 GO:0048232 GO:0048285 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048609 GO:0048646 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051090 GO:0051091 GO:0051128 GO:0051129 GO:0051171 GO:0051252 GO:0051301 GO:0051321 GO:0051493 GO:0051494 GO:0051593 GO:0051704 GO:0051716 GO:0060176 GO:0060255 GO:0060271 GO:0060491 GO:0061640 GO:0065007 GO:0065008 GO:0065009 GO:0070925 GO:0071704 GO:0071840 GO:0080090 GO:0080134 GO:0080135 GO:0090066 GO:0097159 GO:0097367 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0110053 GO:0120031 GO:0120032 GO:0120035 GO:0120036 GO:0140013 GO:0140096 GO:1901265 GO:1901363 GO:1901564 GO:1901698 GO:1901700 GO:1902410 GO:1902903 GO:1902904 GO:1903046 GO:1903047 GO:1903506 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04341 map04341
Pfam Domains
Protein Families
Arm HEAT HEAT_2 Pkinase

Protein Analysis

1331

Amino Acids

144.63

Weight (kDa)

8.29

Isoelectric Point (pI)

48.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 6 - 256 1.1e-78 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 8 - 251 3.4e-47 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 3674
Acc16I TGCGCA 1 cut(s) 1781
Acc36I ACCTGC 1 cut(s) 3674
AccB1I GGYRCC 2 cut(s) 1238, 2510
AccI GTMKAC 2 cut(s) 60, 1374
AccII CGCG 2 cut(s) 1678, 2450
AciI CCGC 8 cut(s) 1766, 2448, 3579, 3613, 3706, 3878, 3934, 3972
AcoI YGGCCR 2 cut(s) 592, 740
AcsI RAATTY 9 cut(s) 104, 230, 273, 613, 682, 1044, 1281, 2230, 3551
AcuI CTGAAG 4 cut(s) 719, 801, 891, 3785
AcvI CACGTG 1 cut(s) 449
AdeI CACNNNGTG 4 cut(s) 260, 1934, 2459, 3026
AfaI GTAC 5 cut(s) 493, 503, 522, 1075, 3544
AfiI CCNNNNNNNGG 9 cut(s) 505, 991, 2569, 2871, 2882, 3106, 3211, 3923, 3943
AflIII ACRYGT 1 cut(s) 1123
AhlI ACTAGT 2 cut(s) 517, 1840
AjnI CCWGG 7 cut(s) 1000, 1984, 2864, 2875, 2962, 3022, 3118
AjuI GAANNNNNNNTTGG 6 cut(s) 33, 65, 573, 605, 1565, 1597
Alw21I GWGCWC 6 cut(s) 1090, 2321, 3442, 3691, 3785, 3872
Alw26I GTCTC 7 cut(s) 564, 767, 1628, 2120, 3045, 3512, 3742
Alw44I GTGCAC 2 cut(s) 1086, 3687
AlwNI CAGNNNCTG 5 cut(s) 1016, 1416, 1537, 2794, 2981
AoxI GGCC 8 cut(s) 592, 740, 1999, 2560, 2571, 2591, 2873, 3177
ApaI GGGCCC 1 cut(s) 2595
ApaLI GTGCAC 2 cut(s) 1086, 3687
ApeKI GCWGC 8 cut(s) 839, 1232, 1962, 2269, 2348, 2351, 3610, 3680
ApoI RAATTY 9 cut(s) 104, 230, 273, 613, 682, 1044, 1281, 2230, 3551
ArsI GACNNNNNNTTYG 8 cut(s) 425, 457, 1084, 1116, 3490, 3522, 3720, 3752
AseI ATTAAT 1 cut(s) 3360
Asp700I GAANNNNTTC 3 cut(s) 309, 1216, 2230
AspLEI GCGC 2 cut(s) 1782, 3823
AspS9I GGNCC 6 cut(s) 1661, 2406, 2501, 2591, 2592, 2873
AsuC2I CCSGG 1 cut(s) 3944
AsuII TTCGAA 1 cut(s) 3425
AvaII GGWCC 3 cut(s) 1661, 2406, 2501
BaeGI GKGCMC 3 cut(s) 1090, 2595, 3691
BalI TGGCCA 2 cut(s) 594, 742
BamHI GGATCC 4 cut(s) 649, 1157, 3000, 3244
BanI GGYRCC 2 cut(s) 1238, 2510
BanII GRGCYC 2 cut(s) 1798, 2595
BbrPI CACGTG 1 cut(s) 449
BbsI GAAGAC 6 cut(s) 76, 3051, 3081, 3175, 3666, 3735
Bbv12I GWGCWC 6 cut(s) 1090, 2321, 3442, 3691, 3785, 3872
BbvI GCAGC 8 cut(s) 851, 1244, 1949, 2281, 2360, 2363, 3597, 3692
BccI CCATC 9 cut(s) 381, 1250, 1901, 2042, 2543, 2577, 2885, 2980, 3983
BceAI ACGGC 1 cut(s) 3944
BcgI CGANNNNNNTGC 2 cut(s) 2258, 2292
BciT130I CCWGG 7 cut(s) 1002, 1986, 2866, 2877, 2964, 3024, 3120
BclI TGATCA 1 cut(s) 2701
BcnI CCSGG 1 cut(s) 3944
BcoDI GTCTC 7 cut(s) 564, 767, 1628, 2120, 3045, 3512, 3742
BcuI ACTAGT 2 cut(s) 517, 1840
BfmI CTRYAG 4 cut(s) 546, 819, 1848, 3883
BfuAI ACCTGC 1 cut(s) 3674
BglI GCCNNNNNGGC 2 cut(s) 2360, 3185
BglII AGATCT 2 cut(s) 1871, 3617
BisI GCNGC 9 cut(s) 840, 1233, 1963, 2270, 2349, 2352, 3579, 3611, 3681
BlpI GCTNAGC 2 cut(s) 324, 1630
BlsI GCNGC 9 cut(s) 841, 1234, 1964, 2271, 2350, 2353, 3580, 3612, 3682
Bme1390I CCNGG 8 cut(s) 1002, 1986, 2866, 2877, 2964, 3024, 3120, 3944
Bme18I GGWCC 3 cut(s) 1661, 2406, 2501
BmgT120I GGNCC 6 cut(s) 1661, 2406, 2501, 2591, 2592, 2873
BmrFI CCNGG 8 cut(s) 1002, 1986, 2866, 2877, 2964, 3024, 3120, 3944
BmrI ACTGGG 2 cut(s) 93, 1426
BmuI ACTGGG 2 cut(s) 93, 1426
BpiI GAAGAC 6 cut(s) 76, 3051, 3081, 3175, 3666, 3735
BplI GAGNNNNNCTC 4 cut(s) 2057, 2089, 3879, 3911
BpmI CTGGAG 5 cut(s) 809, 1662, 2412, 2588, 3398
Bpu1102I GCTNAGC 2 cut(s) 324, 1630
Bpu14I TTCGAA 1 cut(s) 3425
BpuEI CTTGAG 3 cut(s) 1782, 1940, 2664
BpuMI CCSGG 1 cut(s) 3944
Bsa29I ATCGAT 1 cut(s) 3520
BsaAI YACGTR 1 cut(s) 449
BsaBI GATNNNNATC 1 cut(s) 2971
BsaI GGTCTC 2 cut(s) 564, 3512
BsaJI CCNNGG 4 cut(s) 1001, 2595, 2865, 3942
BsaWI WCCGGW 1 cut(s) 2309
BsaXI ACNNNNNCTCC 4 cut(s) 2385, 2415, 3953, 3983
Bsc4I CCNNNNNNNGG 9 cut(s) 505, 991, 2569, 2871, 2882, 3106, 3211, 3923, 3943
Bse118I RCCGGY 1 cut(s) 3456
Bse3DI GCAATG 5 cut(s) 105, 341, 459, 1377, 3580
Bse8I GATNNNNATC 1 cut(s) 2971
BseBI CCWGG 7 cut(s) 1002, 1986, 2866, 2877, 2964, 3024, 3120
BseCI ATCGAT 1 cut(s) 3520
BseDI CCNNGG 4 cut(s) 1001, 2595, 2865, 3942
BseJI GATNNNNATC 1 cut(s) 2971
BseLI CCNNNNNNNGG 9 cut(s) 505, 991, 2569, 2871, 2882, 3106, 3211, 3923, 3943
BseMI GCAATG 5 cut(s) 105, 341, 459, 1377, 3580
BseMII CTCAG 6 cut(s) 1368, 2570, 3000, 3645, 3798, 3897
BseRI GAGGAG 5 cut(s) 1007, 1821, 2667, 2810, 3759
BseSI GKGCMC 3 cut(s) 1090, 2595, 3691
BseXI GCAGC 8 cut(s) 851, 1244, 1949, 2281, 2360, 2363, 3597, 3692
BseYI CCCAGC 1 cut(s) 3627
BsgI GTGCAG 1 cut(s) 2367
Bsh1236I CGCG 2 cut(s) 1678, 2450
Bsh1285I CGRYCG 1 cut(s) 3274
BshFI GGCC 8 cut(s) 594, 742, 2001, 2562, 2573, 2593, 2875, 3179
BshNI GGYRCC 2 cut(s) 1238, 2510
BshVI ATCGAT 1 cut(s) 3520
BsiEI CGRYCG 1 cut(s) 3274
BsiHKAI GWGCWC 6 cut(s) 1090, 2321, 3442, 3691, 3785, 3872
BsiSI CCGG 3 cut(s) 2310, 3457, 3944
BslFI GGGAC 1 cut(s) 2365
BslI CCNNNNNNNGG 9 cut(s) 505, 991, 2569, 2871, 2882, 3106, 3211, 3923, 3943
BsmAI GTCTC 7 cut(s) 564, 767, 1628, 2120, 3045, 3512, 3742
BsmFI GGGAC 1 cut(s) 2365
BsmI GAATGC 4 cut(s) 1989, 2176, 2468, 2962
BsnI GGCC 8 cut(s) 594, 742, 2001, 2562, 2573, 2593, 2875, 3179
Bso31I GGTCTC 2 cut(s) 564, 3512
Bsp119I TTCGAA 1 cut(s) 3425
Bsp120I GGGCCC 1 cut(s) 2591
Bsp1286I GDGCHC 8 cut(s) 1090, 1798, 2321, 2595, 3442, 3691, 3785, 3872
Bsp1407I TGTACA 1 cut(s) 501
Bsp1720I GCTNAGC 2 cut(s) 324, 1630
BspACI CCGC 8 cut(s) 1766, 2448, 3579, 3613, 3706, 3878, 3934, 3972
BspANI GGCC 8 cut(s) 594, 742, 2001, 2562, 2573, 2593, 2875, 3179
BspCNI CTCAG 6 cut(s) 1367, 2571, 2999, 3646, 3797, 3898
BspDI ATCGAT 1 cut(s) 3520
BspFNI CGCG 2 cut(s) 1678, 2450
BspMAI CTGCAG 3 cut(s) 550, 823, 3887
BspMI ACCTGC 1 cut(s) 3674
BspQI GCTCTTC 1 cut(s) 3600
BspT104I TTCGAA 1 cut(s) 3425
BspT107I GGYRCC 2 cut(s) 1238, 2510
BspTNI GGTCTC 2 cut(s) 564, 3512
BsrDI GCAATG 5 cut(s) 105, 341, 459, 1377, 3580
BsrFI RCCGGY 1 cut(s) 3456
BsrGI TGTACA 1 cut(s) 501
BssAI RCCGGY 1 cut(s) 3456
BssECI CCNNGG 4 cut(s) 1001, 2595, 2865, 3942
BssNAI GTATAC 1 cut(s) 61
BssT1I CCWWGG 1 cut(s) 2595
Bst1107I GTATAC 1 cut(s) 61
Bst2UI CCWGG 7 cut(s) 1002, 1986, 2866, 2877, 2964, 3024, 3120
Bst6I CTCTTC 2 cut(s) 1471, 3600
BstAPI GCANNNNNTGC 5 cut(s) 1238, 2201, 3453, 3686, 3760
BstAUI TGTACA 1 cut(s) 501
BstBAI YACGTR 1 cut(s) 449
BstBI TTCGAA 1 cut(s) 3425
BstEII GGTNACC 1 cut(s) 3200
BstENI CCTNNNNNAGG 3 cut(s) 2567, 2880, 3104
BstFNI CGCG 2 cut(s) 1678, 2450
BstHHI GCGC 2 cut(s) 1782, 3823
BstMAI GTCTC 7 cut(s) 564, 767, 1628, 2120, 3045, 3512, 3742
BstMCI CGRYCG 1 cut(s) 3274
BstNI CCWGG 7 cut(s) 1002, 1986, 2866, 2877, 2964, 3024, 3120
BstNSI RCATGY 3 cut(s) 1127, 1698, 2845
BstPI GGTNACC 1 cut(s) 3200
BstSCI CCNGG 8 cut(s) 1000, 1984, 2864, 2875, 2962, 3022, 3118, 3942
BstSFI CTRYAG 4 cut(s) 546, 819, 1848, 3883
BstSLI GKGCMC 3 cut(s) 1090, 2595, 3691
BstUI CGCG 2 cut(s) 1678, 2450
BstV1I GCAGC 8 cut(s) 851, 1244, 1949, 2281, 2360, 2363, 3597, 3692
BstV2I GAAGAC 6 cut(s) 76, 3051, 3081, 3175, 3666, 3735
BstX2I RGATCY 9 cut(s) 649, 1012, 1157, 1871, 2110, 3000, 3244, 3409, 3617
BstXI CCANNNNNNTGG 1 cut(s) 2167
BstYI RGATCY 9 cut(s) 649, 1012, 1157, 1871, 2110, 3000, 3244, 3409, 3617
BstZ17I GTATAC 1 cut(s) 61
Bsu15I ATCGAT 1 cut(s) 3520
BsuRI GGCC 8 cut(s) 594, 742, 2001, 2562, 2573, 2593, 2875, 3179
BsuTUI ATCGAT 1 cut(s) 3520
BtsI GCAGTG 5 cut(s) 543, 1465, 1575, 2433, 3442
BveI ACCTGC 1 cut(s) 3674
CaiI CAGNNNCTG 5 cut(s) 1016, 1416, 1537, 2794, 2981
CfoI GCGC 2 cut(s) 1782, 3823
Cfr10I RCCGGY 1 cut(s) 3456
Cfr13I GGNCC 6 cut(s) 1661, 2406, 2501, 2591, 2592, 2873
ClaI ATCGAT 1 cut(s) 3520
CseI GACGC 1 cut(s) 81
CsiI ACCWGGT 1 cut(s) 3022
Csp6I GTAC 5 cut(s) 492, 502, 521, 1074, 3543
CspCI CAANNNNNGTGG 2 cut(s) 1285, 1320
CviQI GTAC 5 cut(s) 492, 502, 521, 1074, 3543
DraI TTTAAA 2 cut(s) 688, 2214
DraIII CACNNNGTG 4 cut(s) 260, 1934, 2459, 3026
EaeI YGGCCR 2 cut(s) 592, 740
Eam1104I CTCTTC 2 cut(s) 1471, 3600
EarI CTCTTC 2 cut(s) 1471, 3600
EciI GGCGGA 3 cut(s) 3867, 3923, 3961
Eco130I CCWWGG 1 cut(s) 2595
Eco24I GRGCYC 2 cut(s) 1798, 2595
Eco31I GGTCTC 2 cut(s) 564, 3512
Eco32I GATATC 2 cut(s) 139, 2835
Eco47I GGWCC 3 cut(s) 1661, 2406, 2501
Eco57I CTGAAG 4 cut(s) 719, 801, 891, 3785
Eco72I CACGTG 1 cut(s) 449
Eco91I GGTNACC 1 cut(s) 3200
EcoNI CCTNNNNNAGG 3 cut(s) 2567, 2880, 3104
EcoO109I RGGNCCY 5 cut(s) 1661, 2406, 2501, 2591, 2592
EcoO65I GGTNACC 1 cut(s) 3200
EcoRI GAATTC 1 cut(s) 230
EcoRII CCWGG 7 cut(s) 1000, 1984, 2864, 2875, 2962, 3022, 3118
EcoRV GATATC 2 cut(s) 139, 2835
EcoT14I CCWWGG 1 cut(s) 2595
EcoT22I ATGCAT 3 cut(s) 1471, 1726, 2847
EcoT38I GRGCYC 2 cut(s) 1798, 2595
ErhI CCWWGG 1 cut(s) 2595
FalI AAGNNNNNCTT 4 cut(s) 1173, 1205, 2697, 2729
FaqI GGGAC 1 cut(s) 2365
FbaI TGATCA 1 cut(s) 2701
FblI GTMKAC 2 cut(s) 60, 1374
Fnu4HI GCNGC 9 cut(s) 840, 1233, 1963, 2270, 2349, 2352, 3579, 3611, 3681
FriOI GRGCYC 2 cut(s) 1798, 2595
Fsp4HI GCNGC 9 cut(s) 840, 1233, 1963, 2270, 2349, 2352, 3579, 3611, 3681
FspI TGCGCA 1 cut(s) 1781
GlaI GCGC 2 cut(s) 1781, 3822
GluI GCNGC 9 cut(s) 840, 1233, 1963, 2270, 2349, 2352, 3579, 3611, 3681
GsaI CCCAGC 1 cut(s) 3631
GsuI CTGGAG 5 cut(s) 809, 1662, 2412, 2588, 3398
HaeIII GGCC 8 cut(s) 594, 742, 2001, 2562, 2573, 2593, 2875, 3179
HapII CCGG 3 cut(s) 2310, 3457, 3944
HgaI GACGC 1 cut(s) 81
HhaI GCGC 2 cut(s) 1782, 3823
Hin6I GCGC 2 cut(s) 1780, 3821
HinP1I GCGC 2 cut(s) 1780, 3821
HincII GTYRAC 2 cut(s) 1518, 1913
HindII GTYRAC 2 cut(s) 1518, 1913
HpaI GTTAAC 1 cut(s) 1518
HpaII CCGG 3 cut(s) 2310, 3457, 3944
HpyCH4IV ACGT 3 cut(s) 448, 1915, 3541
HpySE526I ACGT 3 cut(s) 448, 1915, 3541
HspAI GCGC 2 cut(s) 1780, 3821
KroI GCCGGC 1 cut(s) 3456
KroNI GCCGGC 1 cut(s) 3458
Ksp22I TGATCA 1 cut(s) 2701
KspAI GTTAAC 1 cut(s) 1518
LguI GCTCTTC 1 cut(s) 3600
LmnI GCTCC 8 cut(s) 514, 1643, 2393, 2771, 2965, 3470, 3746, 3936
Lsp1109I GCAGC 8 cut(s) 851, 1244, 1949, 2281, 2360, 2363, 3597, 3692
MabI ACCWGGT 1 cut(s) 3022
MaeII ACGT 3 cut(s) 448, 1915, 3541
MaeIII GTNAC 7 cut(s) 241, 377, 431, 1852, 2020, 3200, 3828
MfeI CAATTG 4 cut(s) 318, 329, 2121, 3954
MflI RGATCY 9 cut(s) 649, 1012, 1157, 1871, 2110, 3000, 3244, 3409, 3617
MhlI GDGCHC 8 cut(s) 1090, 1798, 2321, 2595, 3442, 3691, 3785, 3872
MlsI TGGCCA 2 cut(s) 594, 742
MluNI TGGCCA 2 cut(s) 594, 742
MlyI GAGTC 3 cut(s) 956, 1499, 3836
MmeI TCCRAC 3 cut(s) 3091, 3222, 3738
Mox20I TGGCCA 2 cut(s) 594, 742
Mph1103I ATGCAT 3 cut(s) 1471, 1726, 2847
MroNI GCCGGC 1 cut(s) 3456
MroXI GAANNNNTTC 3 cut(s) 309, 1216, 2230
MscI TGGCCA 2 cut(s) 594, 742
MslI CAYNNNNRTG 1 cut(s) 200
Msp20I TGGCCA 2 cut(s) 594, 742
MspA1I CMGCKG 1 cut(s) 1235
MspI CCGG 3 cut(s) 2310, 3457, 3944
MspR9I CCNGG 8 cut(s) 1002, 1986, 2866, 2877, 2964, 3024, 3120, 3944
MunI CAATTG 4 cut(s) 318, 329, 2121, 3954
Mva1269I GAATGC 4 cut(s) 1989, 2176, 2468, 2962
MvaI CCWGG 7 cut(s) 1002, 1986, 2866, 2877, 2964, 3024, 3120
MvnI CGCG 2 cut(s) 1678, 2450
NaeI GCCGGC 1 cut(s) 3458
NciI CCSGG 1 cut(s) 3944
NgoMIV GCCGGC 1 cut(s) 3456
NmuCI GTSAC 5 cut(s) 241, 377, 431, 3200, 3828
NsbI TGCGCA 1 cut(s) 1781
NsiI ATGCAT 3 cut(s) 1471, 1726, 2847
NspI RCATGY 3 cut(s) 1127, 1698, 2845
NspV TTCGAA 1 cut(s) 3425
PaeI GCATGC 1 cut(s) 1698
PaqCI CACCTGC 1 cut(s) 3674
PciI ACATGT 1 cut(s) 1123
PciSI GCTCTTC 1 cut(s) 3600
PctI GAATGC 4 cut(s) 1989, 2176, 2468, 2962
PdiI GCCGGC 1 cut(s) 3458
PdmI GAANNNNTTC 3 cut(s) 309, 1216, 2230
PflFI GACNNNGTC 1 cut(s) 3734
PfoI TCCNGGA 1 cut(s) 2962
PkrI GCNGC 9 cut(s) 841, 1234, 1964, 2271, 2350, 2353, 3580, 3612, 3682
Ple19I CGATCG 1 cut(s) 3274
PleI GAGTC 3 cut(s) 956, 1498, 3835
PmaCI CACGTG 1 cut(s) 449
PmlI CACGTG 1 cut(s) 449
PpsI GAGTC 3 cut(s) 956, 1498, 3835
Ppu21I YACGTR 1 cut(s) 449
PpuMI RGGWCCY 3 cut(s) 1661, 2406, 2501
PscI ACATGT 1 cut(s) 1123
PshBI ATTAAT 1 cut(s) 3360
Psp5II RGGWCCY 3 cut(s) 1661, 2406, 2501
Psp6I CCWGG 7 cut(s) 1000, 1984, 2864, 2875, 2962, 3022, 3118
PspCI CACGTG 1 cut(s) 449
PspEI GGTNACC 1 cut(s) 3200
PspFI CCCAGC 1 cut(s) 3627
PspGI CCWGG 7 cut(s) 1000, 1984, 2864, 2875, 2962, 3022, 3118
PspOMI GGGCCC 1 cut(s) 2591
PspPI GGNCC 6 cut(s) 1661, 2406, 2501, 2591, 2592, 2873
PspPPI RGGWCCY 3 cut(s) 1661, 2406, 2501
PstI CTGCAG 3 cut(s) 550, 823, 3887
PstNI CAGNNNCTG 5 cut(s) 1016, 1416, 1537, 2794, 2981
PsuI RGATCY 9 cut(s) 649, 1012, 1157, 1871, 2110, 3000, 3244, 3409, 3617
PsyI GACNNNGTC 1 cut(s) 3734
PvuI CGATCG 1 cut(s) 3274
PvuII CAGCTG 1 cut(s) 1235
RsaI GTAC 5 cut(s) 493, 503, 522, 1075, 3544
RsaNI GTAC 5 cut(s) 492, 502, 521, 1074, 3543
RseI CAYNNNNRTG 1 cut(s) 200
SapI GCTCTTC 1 cut(s) 3600
SatI GCNGC 9 cut(s) 840, 1233, 1963, 2270, 2349, 2352, 3579, 3611, 3681
Sau96I GGNCC 6 cut(s) 1661, 2406, 2501, 2591, 2592, 2873
SchI GAGTC 3 cut(s) 956, 1499, 3836
ScrFI CCNGG 8 cut(s) 1002, 1986, 2866, 2877, 2964, 3024, 3120, 3944
SduI GDGCHC 8 cut(s) 1090, 1798, 2321, 2595, 3442, 3691, 3785, 3872
SexAI ACCWGGT 1 cut(s) 3022
SfcI CTRYAG 4 cut(s) 546, 819, 1848, 3883
SfuI TTCGAA 1 cut(s) 3425
SinI GGWCC 3 cut(s) 1661, 2406, 2501
SmiMI CAYNNNNRTG 1 cut(s) 200
SmlI CTYRAG 3 cut(s) 1797, 1919, 2679
SmoI CTYRAG 3 cut(s) 1797, 1919, 2679
SpeI ACTAGT 2 cut(s) 517, 1840
SphI GCATGC 1 cut(s) 1698
SsiI CCGC 8 cut(s) 1766, 2448, 3579, 3613, 3706, 3878, 3934, 3972
SspI AATATT 1 cut(s) 955
StyD4I CCNGG 8 cut(s) 1000, 1984, 2864, 2875, 2962, 3022, 3118, 3942
StyI CCWWGG 1 cut(s) 2595
TaiI ACGT 3 cut(s) 451, 1918, 3544
TatI WGTACW 1 cut(s) 501
TauI GCSGC 1 cut(s) 3581
TseFI GTSAC 5 cut(s) 241, 377, 431, 3200, 3828
TseI GCWGC 8 cut(s) 839, 1232, 1962, 2269, 2348, 2351, 3610, 3680
Tsp45I GTSAC 5 cut(s) 241, 377, 431, 3200, 3828
TspGWI ACGGA 2 cut(s) 3562, 3887
Tth111I GACNNNGTC 1 cut(s) 3734
VneI GTGCAC 2 cut(s) 1086, 3687
VpaK11BI GGWCC 3 cut(s) 1661, 2406, 2501
VspI ATTAAT 1 cut(s) 3360
XagI CCTNNNNNAGG 3 cut(s) 2567, 2880, 3104
XapI RAATTY 9 cut(s) 104, 230, 273, 613, 682, 1044, 1281, 2230, 3551
XbaI TCTAGA 2 cut(s) 3139, 3740
XceI RCATGY 3 cut(s) 1127, 1698, 2845
XcmI CCANNNNNNNNNTGG 3 cut(s) 875, 1303, 2362
XmiI GTMKAC 2 cut(s) 60, 1374
XmnI GAANNNNTTC 3 cut(s) 309, 1216, 2230
Zsp2I ATGCAT 3 cut(s) 1471, 1726, 2847
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.