Prupe.3G137600_v2.0.a1

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0004502
Physical Location & Seq
Forward (+)
14527314 .. 14536935
9622 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G137600.3

Sequence Viewer

Length: 4047 bp
ATGGGTGTGGAGGGCTACCATGTGATTGAGCTTGTAGGCGAAGGTTCATTTGGGAAGGTATACAAGGGAAGGCGCAAGTATACTGGGCAGACAGTTGCAATGAAATTCATTATGAAACATGGGAAAACCGACAAAGATATTCACAATTTAAGACAAGAAATTGAGATCCTAAGAAAGTTGAAGCATGAGAATATAATTGAAATGCTTGATTCCTTTGAAAGCCCACAAGAGTTTTGTGTTGTCACAGAATTTGCACAAGGTGAATTATTTGAAATTGTTGAGGATGATAAGTGCCTTCATGAAGAACAAGTTCAAGCAATTGCAAAGCAGTTGGTCAGAGCATTGCATTATTTGCATTCCAACCGTATCATCCATCGTGACATGAAACCACAAAACATTCTCATTGGTGCTGGCTCTATTGTTAAGCTCTGTGATTTTGGGTTTGCACGTGCAATGTCCACAAATACTGTTGTTTTGCGATCCATTAAAGGTACTCCTCTGTACATGGCACCTGAACTAGTACGGGAACAACCCTACAATCACACCGCAGATTTATGGTCTCTTGGAGTAATCTTATATGAATTATTTGTTGGCCAGCCTCCTTTCTATACAAATTCTATATATGCACTCATCAGGCACATTGTTAAGGATCCGGTTAAATACCCTGACAACATGAGTCCAAGTTTTAAAAGCTTTCTGAAGGGATTGCTCAATAAGGTACCTCAAAATCGATTGACTTGGCCAGCTCTTCTTGAACACCCATTTGTTAAAGAGACGTCTCATGAACTGGAGGCTAGGGAAATGCATTCTGCAACTGCTGCAGAAAGAGGATGTGTTTCTGCATGGAGGCGTGAAGGAAACACAGTTCAAACATCAGTTGGTTTGGCTGTTTCTTCTTCTGTCAACTCTACAGCTTCTTCAGAGAACAGTGGAATAAGTTTTCAGAATGATGCTCAATCAGACATTTATGACTGCACGACAGTCAATTATTCCCCAAATGAGTTCCCAGGATTTGCAAATCTTAAGGAAGTTAAACAGTCAGGTTGCCAAATTTTGGACAGATTGGAAAATAACTCTGGTACAGTCAAAGGTGCACAAGTAATTGGTCAAGACAATGAAGCATTAGCACAGGTTTTGCTACCACTAAAAAGATGTTCCCAAGGATCCCTAGATTCTTCCTGGGATGAAGATATTCTTAATTCTAACCAGTCTTTGAGAATCCTTTCAAATTTAGTTGCAGCTGGTGCCATCCATTCCAGTGCGCTGCTTGATGAAATTATACATGAACTTCTTGTTTACACTGGCATCATTGTGAGCATGAAAACTTCTGAAGTAAATGAGTTAAAAGAAAAGAGCTTCTCAATAATTAAATTTTTGGTAGACAATGCCGGAATTGGCACTGGAGACTCATATTTCAGGCACTGGGTTGCTTTAACTGATATTTTTTCGCAGGTTGTTGGTTGCAGTGAAGATGCGTCTGGAAGAGTACTGTATGCGTCAATTGCTTGCATTACTGTAGTGTTAACAAGACTCACTCAGGGCCTTAAAGCATGTTCTTCAACTTCAGCTCCTGAGGCGGTTTCTGATCTTAATGGAACGTTGAAACGTATTTTGGATCGCGCTAAAACATGTGGTCTAGTGGATCAACTTTGTCTCTGCTTAGTTACTGCAGGGTCAAGTCTTATTTCTGGTTCTTCAAATATGCTGTGTGCAGCTTGTGAAGCATGTAGGGCTATTTGGTTGCTGATAGATGCATCCGAAAATATTTGTACAAAAAGAAATGCCTATTCATTTCCCTTAAGCACTTTGCGCAGTCCTTCACTCCAATTTGACATTAGGGATCAAGATCAAATTTCATTAATTGGGACAGAATCCACAAAACTTGTTGCTGCAGTGACTAGAGCATTCCTTAGATCAGAAGCAGTACAGGTTGCTATCCGTTATTGTCTTCATCAACGACTTGAAGCTTCACTGTATGCTAGCATTCAGCTGTTGTTGAGGTGCTGCTTGCATAATGGAACTGTTCCCGGTATGCTCTGTGGCCTGCCTAGTTCCCTACCTGTAACTACTGTTGTCAGTGGTGGGGGTGATGGAACCATTATTTCAGAGATATTCTCTTTGTTGTCTTTGTGCATGTCATCTCAAAATAAAGATCCACAAGCAATGGAGACAACCAACTTCAAGTGCAAATTAACCAATCCCACTACCTTCATTCTGCATTCATGCCTCATCCTTGCAACAATTGCACAATGTTTGAAGGCAACTGGTAGACATTCTGCATTATTTATGTTGACAACCTCCCCAAAGAAGCAGCTTTCTCGACTTTCTGTCCTTGCTCATCATTTTTCTTCTGATGAGACCACAAACACTTCCTTTCAAACTCATACTGCATCAGCCATGCTGGCACTGGCTTCCATATTGTCGCTTGAATCTGGAGCTTCTGTTGGCTCATCTGTCTCCAGGGTAGCAGTGCCTTTGATTCCTCGAAGTACCACACTATGTGAATACCTCAAACTTTCATCAGGCAATGGAATTGAATTGGGTCCTAATGACACCAATGGTGCTCTCTCATACTGGCATGGTCTTCGGGATGGATGTGTTGGCTTGCTGGAGTCCAGACTGAGGTGGGGAGGACCCGTATCCATAAAACAGCTGTGTGCAAGCAATATCCCTCTGCTTCTAGTCAGCTTGTTAACCAAAAACCAACAAAATGTGTCTCCACAAGAAGTTGACAGCACAAATGATCAAGTTGGGCTTTCCCCTATAGGAGTTGTGTCGACAATATCGTCAATATGTCACTGCCTCTCAGGTGGAGCCTTAACTTTTCGTCAGGTTCTGCTCAGAAGTGATCACATCAAGAATATCTCTGACTTGATATCTGATATGCATCTCAAGCTTGTAAAGTCCTGGGTTGGGCCTGGTGGAGGGAAGGATGGTGTGAGAGATATAATAAATACAGTAATTGATCTCTTGGCATTTCCTTTTGTGACCGTACAGAATGCTCCAGGATTTCCATCAGCCACTGCTTCAGTGAACAGCGGGGCCCTTCTCAACATGGGTTCACCAGGTGTGAGAGTTGGCATGGAAGACAAAGACATGGTGAAAGTAATTGAGGAAGACCTGGGAAAGTATATTAAAAACCTTTTGGAGGTAAGAGTGCCAGGCATCATTCTTCGATGTTTAGAGCATCTGGAGTTAAAGGATACAGGAAGGCCTGTGGCCTTTCTTGCTAAAATGATTGGTCACCAATCATTGGCACTTCAACTTGTGCGTAAAGAAGGCTTGTTGGAGCCTACTAGAATGAGAAGATTACTCGATTGTTCAAGCCCAAGAGAGGTGGTGCTGGATGTTCTGATGATTGTTTCTGATTTAGCTCGCAAGGATGAGGGATTCTACGGATGTATTAATGGAGCTTCTGTACTGGAGTTCTTCAAGGAATTTCTTACCCATGAAGATCCCAATGTGCGGTCAAAGGCTTGCAGTGCTCTGGGGAATATGTGCCGGCACAGCTCCTACTTTTATAGTGCCCTAGCAAGGCATCAAATTATTGGCCTCCTTCTTGATCGATGCTCTGATCCAGACAAACGGACACGAAAATTTGCTTGCTTTGCTATTGGGAATGCTGCCTACCATAGTGACATGTTATATGATGAGCTACGAAGATCTATACCCCATCTTGCCAAATTTCTGGTTTCAACTGAAGAAGACAAGACTAAAGCAAATGCTGCAGCTGCACTAAGCAATCTTGTCCGCAACTCCGACAAACTTTGTGAAGATATTGTATCAAAAGGAGCCATGCAGTCTTTACTGAAGTTGGTGGCGGACTGTTCAGTGGTAGCACTGAACCCAGGTAGGAAAGATTCGGTAAATGAGTCACCTTTAAAAATAGCTCTCCTTTCATTGGCAAAGATGTGTTCGCATCCGCCCTGCAGACAATTCCTCCGTTCATCAGAGTTGTTCTCAGTGATTGGACGGCTTCAGCAATCTCCAGAATCAAGAATTGCCAATTACGCCACTGACATTATCTCCAAAATTGCTGATTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000166 GO:0000278 GO:0000280 GO:0000281 GO:0000910 GO:0000911 GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005856 GO:0005874 GO:0005881 GO:0006355 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006935 GO:0006996 GO:0007049 GO:0007112 GO:0007140 GO:0007154 GO:0007163 GO:0007165 GO:0007166 GO:0007224 GO:0007227 GO:0007228 GO:0007275 GO:0007276 GO:0007349 GO:0007584 GO:0008064 GO:0008092 GO:0008134 GO:0008144 GO:0008150 GO:0008152 GO:0008589 GO:0009524 GO:0009553 GO:0009558 GO:0009561 GO:0009605 GO:0009653 GO:0009791 GO:0009889 GO:0009966 GO:0009967 GO:0009987 GO:0009991 GO:0010033 GO:0010243 GO:0010468 GO:0010556 GO:0010639 GO:0010646 GO:0010647 GO:0014070 GO:0015630 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017076 GO:0019219 GO:0019222 GO:0019538 GO:0019894 GO:0019953 GO:0022402 GO:0022412 GO:0022414 GO:0022607 GO:0023051 GO:0023052 GO:0023056 GO:0030010 GO:0030030 GO:0030031 GO:0030554 GO:0030832 GO:0030833 GO:0030837 GO:0031156 GO:0031272 GO:0031323 GO:0031326 GO:0031333 GO:0031344 GO:0031667 GO:0032101 GO:0032104 GO:0032107 GO:0032271 GO:0032272 GO:0032501 GO:0032502 GO:0032504 GO:0032506 GO:0032535 GO:0032553 GO:0032555 GO:0032559 GO:0032956 GO:0032970 GO:0033043 GO:0033206 GO:0033273 GO:0035556 GO:0035639 GO:0036094 GO:0036211 GO:0040011 GO:0042221 GO:0042330 GO:0042493 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043254 GO:0043326 GO:0043327 GO:0043412 GO:0043900 GO:0044085 GO:0044087 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0044703 GO:0044782 GO:0045880 GO:0048229 GO:0048232 GO:0048285 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048609 GO:0048646 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051090 GO:0051091 GO:0051128 GO:0051129 GO:0051171 GO:0051252 GO:0051301 GO:0051321 GO:0051493 GO:0051494 GO:0051593 GO:0051704 GO:0051716 GO:0060176 GO:0060255 GO:0060271 GO:0060491 GO:0061640 GO:0065007 GO:0065008 GO:0065009 GO:0070925 GO:0071704 GO:0071840 GO:0080090 GO:0080134 GO:0080135 GO:0090066 GO:0097159 GO:0097367 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0110053 GO:0120031 GO:0120032 GO:0120035 GO:0120036 GO:0140013 GO:0140096 GO:1901265 GO:1901363 GO:1901564 GO:1901698 GO:1901700 GO:1902410 GO:1902903 GO:1902904 GO:1903046 GO:1903047 GO:1903506 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04341 map04341
Pfam Domains
Protein Families
Arm HEAT HEAT_2 Pkinase

Protein Analysis

1349

Amino Acids

147.32

Weight (kDa)

7.31

Isoelectric Point (pI)

47.07

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2785
AatII GACGTC 1 cut(s) 779
Acc16I TGCGCA 1 cut(s) 1811
Acc36I ACCTGC 1 cut(s) 1441
Acc65I GGTACC 1 cut(s) 718
AccB1I GGYRCC 3 cut(s) 508, 718, 1244
AccB7I CCANNNNNTGG 2 cut(s) 1054, 3253
AccI GTMKAC 5 cut(s) 60, 80, 1380, 2269, 2777
AccII CGCG 1 cut(s) 1620
AciI CCGC 7 cut(s) 546, 1577, 3039, 3466, 3751, 3821, 3923
AclI AACGTT 1 cut(s) 1598
AcoI YGGCCR 2 cut(s) 592, 740
AcuI CTGAAG 8 cut(s) 719, 903, 1350, 1548, 3012, 3720, 3830, 3962
AcvI CACGTG 1 cut(s) 449
AcyI GRCGYC 1 cut(s) 776
AdeI CACNNNGTG 3 cut(s) 260, 2501, 3068
AflII CTTAAG 2 cut(s) 1022, 1798
AflIII ACRYGT 2 cut(s) 1628, 3639
AhdI GACNNNNNGTC 1 cut(s) 2327
AhlI ACTAGT 1 cut(s) 517
AjuI GAANNNNNNNTTGG 6 cut(s) 33, 65, 573, 605, 1595, 1627
AloI GAACNNNNNNTCC 2 cut(s) 849, 881
Alw21I GWGCWC 3 cut(s) 1096, 2566, 3487
Alw26I GTCTC 9 cut(s) 564, 767, 783, 1398, 1658, 2162, 2351, 2461, 2721
Alw44I GTGCAC 1 cut(s) 1092
AlwNI CAGNNNCTG 4 cut(s) 1422, 1571, 2836, 3023
AoxI GGCC 9 cut(s) 592, 740, 1540, 2041, 2915, 3042, 3212, 3219, 3550
ApaI GGGCCC 1 cut(s) 3046
ApaLI GTGCAC 1 cut(s) 1092
AseI ATTAAT 2 cut(s) 1859, 3405
Asp700I GAANNNNTTC 3 cut(s) 309, 1191, 1222
Asp718I GGTACC 1 cut(s) 718
AspLEI GCGC 4 cut(s) 75, 1264, 1622, 1812
AspS9I GGNCC 6 cut(s) 1540, 2543, 2633, 2915, 3042, 3043
AsuC2I CCSGG 1 cut(s) 2028
AsuHPI GGTGA 6 cut(s) 272, 2099, 3054, 3112, 3236, 3867
AsuNHI GCTAGC 1 cut(s) 1979
AvaII GGWCC 2 cut(s) 2543, 2633
AxyI CCTNAGG 1 cut(s) 1572
BaeGI GKGCMC 3 cut(s) 1096, 3046, 3529
BalI TGGCCA 2 cut(s) 594, 742
BamHI GGATCC 2 cut(s) 649, 1163
BanI GGYRCC 3 cut(s) 508, 718, 1244
BanII GRGCYC 1 cut(s) 3046
BbrPI CACGTG 1 cut(s) 449
BbsI GAAGAC 5 cut(s) 1940, 2576, 3093, 3123, 3711
Bbv12I GWGCWC 3 cut(s) 1096, 2566, 3487
BccI CCATC 7 cut(s) 381, 1256, 2084, 2585, 2927, 3022, 3681
BceAI ACGGC 1 cut(s) 3989
BcgI CGANNNNNNTGC 4 cut(s) 2300, 2334, 2567, 2601
BciVI GTATCC 2 cut(s) 2650, 3196
BclI TGATCA 2 cut(s) 2743, 2848
BcnI CCSGG 1 cut(s) 2028
BcoDI GTCTC 9 cut(s) 564, 767, 783, 1398, 1658, 2162, 2351, 2461, 2721
BcuI ACTAGT 1 cut(s) 517
BfmI CTRYAG 8 cut(s) 819, 909, 1515, 1668, 1890, 2763, 3726, 3928
BfrI CTTAAG 2 cut(s) 1022, 1798
BfuAI ACCTGC 1 cut(s) 1441
BfuI GTATCC 2 cut(s) 2650, 3196
BglI GCCNNNNNGGC 1 cut(s) 2402
BglII AGATCT 1 cut(s) 3662
BmcAI AGTACT 1 cut(s) 1488
Bme18I GGWCC 2 cut(s) 2543, 2633
BmeRI GACNNNNNGTC 1 cut(s) 2327
BmgT120I GGNCC 6 cut(s) 1540, 2543, 2633, 2915, 3042, 3043
BmrI ACTGGG 2 cut(s) 93, 1432
BmtI GCTAGC 1 cut(s) 1983
BmuI ACTGGG 2 cut(s) 93, 1432
BpiI GAAGAC 5 cut(s) 1940, 2576, 3093, 3123, 3711
BplI GAGNNNNNCTC 4 cut(s) 2099, 2131, 3944, 3976
BpmI CTGGAG 9 cut(s) 809, 1422, 2443, 2454, 2630, 2988, 3212, 3443, 3972
BpuEI CTTGAG 1 cut(s) 2876
BpuMI CCSGG 1 cut(s) 2028
Bsa29I ATCGAT 2 cut(s) 730, 3565
BsaAI YACGTR 1 cut(s) 449
BsaBI GATNNNNATC 3 cut(s) 1845, 2886, 3013
BsaHI GRCGYC 1 cut(s) 776
BsaI GGTCTC 2 cut(s) 564, 2351
BsaJI CCNNGG 7 cut(s) 1006, 1159, 1179, 2460, 2907, 3121, 3847
BsaWI WCCGGW 1 cut(s) 652
Bse118I RCCGGY 1 cut(s) 3501
Bse21I CCTNAGG 1 cut(s) 1572
Bse3DI GCAATG 5 cut(s) 105, 341, 459, 2169, 2533
Bse8I GATNNNNATC 3 cut(s) 1845, 2886, 3013
BseCI ATCGAT 2 cut(s) 730, 3565
BseDI CCNNGG 7 cut(s) 1006, 1159, 1179, 2460, 2907, 3121, 3847
BseJI GATNNNNATC 3 cut(s) 1845, 2886, 3013
BseMI GCAATG 5 cut(s) 105, 341, 459, 2169, 2533
BseMII CTCAG 6 cut(s) 1550, 1563, 2612, 2820, 2854, 3975
BseRI GAGGAG 1 cut(s) 486
BseSI GKGCMC 3 cut(s) 1096, 3046, 3529
BsgI GTGCAG 3 cut(s) 958, 1731, 3717
Bsh1236I CGCG 1 cut(s) 1620
BshFI GGCC 9 cut(s) 594, 742, 1542, 2043, 2917, 3044, 3214, 3221, 3552
BshNI GGYRCC 3 cut(s) 508, 718, 1244
BshVI ATCGAT 2 cut(s) 730, 3565
BsiHKAI GWGCWC 3 cut(s) 1096, 2566, 3487
BsiSI CCGG 4 cut(s) 653, 1389, 2028, 3502
BslFI GGGAC 1 cut(s) 1879
BsmAI GTCTC 9 cut(s) 564, 767, 783, 1398, 1658, 2162, 2351, 2461, 2721
BsmBI CGTCTC 2 cut(s) 767, 783
BsmFI GGGAC 1 cut(s) 1879
BsmI GAATGC 7 cut(s) 355, 805, 1904, 1983, 2218, 3004, 3625
BsnI GGCC 9 cut(s) 594, 742, 1542, 2043, 2917, 3044, 3214, 3221, 3552
Bso31I GGTCTC 2 cut(s) 564, 2351
Bsp120I GGGCCC 1 cut(s) 3042
Bsp1286I GDGCHC 5 cut(s) 1096, 2566, 3046, 3487, 3529
Bsp1407I TGTACA 2 cut(s) 501, 1769
BspACI CCGC 7 cut(s) 546, 1577, 3039, 3466, 3751, 3821, 3923
BspANI GGCC 9 cut(s) 594, 742, 1542, 2043, 2917, 3044, 3214, 3221, 3552
BspCNI CTCAG 6 cut(s) 1549, 1564, 2613, 2819, 2853, 3974
BspDI ATCGAT 2 cut(s) 730, 3565
BspFNI CGCG 1 cut(s) 1620
BspHI TCATGA 2 cut(s) 298, 781
BspMAI CTGCAG 5 cut(s) 823, 1672, 1894, 3730, 3932
BspMI ACCTGC 1 cut(s) 1441
BspOI GCTAGC 1 cut(s) 1983
BspQI GCTCTTC 1 cut(s) 753
BspT107I GGYRCC 3 cut(s) 508, 718, 1244
BspTI CTTAAG 2 cut(s) 1022, 1798
BspTNI GGTCTC 2 cut(s) 564, 2351
BsrDI GCAATG 5 cut(s) 105, 341, 459, 2169, 2533
BsrFI RCCGGY 1 cut(s) 3501
BsrGI TGTACA 2 cut(s) 501, 1769
BssAI RCCGGY 1 cut(s) 3501
BssECI CCNNGG 7 cut(s) 1006, 1159, 1179, 2460, 2907, 3121, 3847
BssNAI GTATAC 2 cut(s) 61, 81
BssNI GRCGYC 1 cut(s) 776
BssT1I CCWWGG 1 cut(s) 1159
Bst1107I GTATAC 2 cut(s) 61, 81
Bst6I CTCTTC 2 cut(s) 753, 1477
BstACI GRCGYC 1 cut(s) 776
BstAFI CTTAAG 2 cut(s) 1022, 1798
BstAPI GCANNNNNTGC 5 cut(s) 352, 818, 1244, 2243, 3725
BstAUI TGTACA 2 cut(s) 501, 1769
BstBAI YACGTR 1 cut(s) 449
BstEII GGTNACC 1 cut(s) 3242
BstENI CCTNNNNNAGG 2 cut(s) 2922, 3146
BstFNI CGCG 1 cut(s) 1620
BstHHI GCGC 4 cut(s) 75, 1264, 1622, 1812
BstMAI GTCTC 9 cut(s) 564, 767, 783, 1398, 1658, 2162, 2351, 2461, 2721
BstNSI RCATGY 5 cut(s) 1554, 1632, 1728, 2137, 3643
BstPI GGTNACC 1 cut(s) 3242
BstSFI CTRYAG 8 cut(s) 819, 909, 1515, 1668, 1890, 2763, 3726, 3928
BstSLI GKGCMC 3 cut(s) 1096, 3046, 3529
BstUI CGCG 1 cut(s) 1620
BstV2I GAAGAC 5 cut(s) 1940, 2576, 3093, 3123, 3711
BstX2I RGATCY 6 cut(s) 165, 649, 1163, 2152, 3454, 3662
BstXI CCANNNNNNTGG 1 cut(s) 3688
BstYI RGATCY 6 cut(s) 165, 649, 1163, 2152, 3454, 3662
BstZ17I GTATAC 2 cut(s) 61, 81
Bsu15I ATCGAT 2 cut(s) 730, 3565
Bsu36I CCTNAGG 1 cut(s) 1572
BsuI GTATCC 2 cut(s) 2650, 3196
BsuRI GGCC 9 cut(s) 594, 742, 1542, 2043, 2917, 3044, 3214, 3221, 3552
BsuTUI ATCGAT 2 cut(s) 730, 3565
BtsI GCAGTG 6 cut(s) 1471, 1899, 2475, 2797, 3021, 3487
BveI ACCTGC 1 cut(s) 1441
CaiI CAGNNNCTG 4 cut(s) 1422, 1571, 2836, 3023
CciI TCATGA 2 cut(s) 298, 781
CfoI GCGC 4 cut(s) 75, 1264, 1622, 1812
Cfr10I RCCGGY 1 cut(s) 3501
Cfr13I GGNCC 6 cut(s) 1540, 2543, 2633, 2915, 3042, 3043
ClaI ATCGAT 2 cut(s) 730, 3565
CseI GACGC 2 cut(s) 1464, 1485
CsiI ACCWGGT 1 cut(s) 3064
CspCI CAANNNNNGTGG 4 cut(s) 1864, 1899, 3318, 3353
DraI TTTAAA 2 cut(s) 688, 3882
DraIII CACNNNGTG 3 cut(s) 260, 2501, 3068
DrdI GACNNNNNNGTC 1 cut(s) 2785
DriI GACNNNNNGTC 1 cut(s) 2327
DseDI GACNNNNNNGTC 1 cut(s) 2785
EaeI YGGCCR 2 cut(s) 592, 740
Eam1104I CTCTTC 2 cut(s) 753, 1477
Eam1105I GACNNNNNGTC 1 cut(s) 2327
EarI CTCTTC 2 cut(s) 753, 1477
EciI GGCGGA 2 cut(s) 3836, 3912
Eco130I CCWWGG 1 cut(s) 1159
Eco147I AGGCCT 1 cut(s) 3214
Eco24I GRGCYC 1 cut(s) 3046
Eco31I GGTCTC 2 cut(s) 564, 2351
Eco32I GATATC 1 cut(s) 2877
Eco47I GGWCC 2 cut(s) 2543, 2633
Eco57I CTGAAG 8 cut(s) 719, 903, 1350, 1548, 3012, 3720, 3830, 3962
Eco72I CACGTG 1 cut(s) 449
Eco81I CCTNAGG 1 cut(s) 1572
Eco91I GGTNACC 1 cut(s) 3242
EcoNI CCTNNNNNAGG 2 cut(s) 2922, 3146
EcoO109I RGGNCCY 5 cut(s) 1540, 2543, 2633, 3042, 3043
EcoO65I GGTNACC 1 cut(s) 3242
EcoRV GATATC 1 cut(s) 2877
EcoT14I CCWWGG 1 cut(s) 1159
EcoT22I ATGCAT 3 cut(s) 807, 1756, 2889
EcoT38I GRGCYC 1 cut(s) 3046
ErhI CCWWGG 1 cut(s) 1159
Esp3I CGTCTC 2 cut(s) 767, 783
FalI AAGNNNNNCTT 4 cut(s) 1179, 1211, 2739, 2771
FaqI GGGAC 1 cut(s) 1879
FauI CCCGC 1 cut(s) 3032
FbaI TGATCA 2 cut(s) 2743, 2848
FblI GTMKAC 5 cut(s) 60, 80, 1380, 2269, 2777
FriOI GRGCYC 1 cut(s) 3046
FspI TGCGCA 1 cut(s) 1811
GlaI GCGC 4 cut(s) 74, 1263, 1621, 1811
GsuI CTGGAG 9 cut(s) 809, 1422, 2443, 2454, 2630, 2988, 3212, 3443, 3972
HaeIII GGCC 9 cut(s) 594, 742, 1542, 2043, 2917, 3044, 3214, 3221, 3552
HapII CCGG 4 cut(s) 653, 1389, 2028, 3502
HgaI GACGC 2 cut(s) 1464, 1485
HhaI GCGC 4 cut(s) 75, 1264, 1622, 1812
Hin1I GRCGYC 1 cut(s) 776
Hin6I GCGC 4 cut(s) 73, 1262, 1620, 1810
HinP1I GCGC 4 cut(s) 73, 1262, 1620, 1810
HincII GTYRAC 6 cut(s) 904, 1524, 2292, 2694, 2731, 2778
HindII GTYRAC 6 cut(s) 904, 1524, 2292, 2694, 2731, 2778
HindIII AAGCTT 3 cut(s) 691, 1965, 2894
HpaI GTTAAC 2 cut(s) 1524, 2694
HpaII CCGG 4 cut(s) 653, 1389, 2028, 3502
HphI GGTGA 6 cut(s) 272, 2099, 3054, 3112, 3236, 3867
HpyCH4IV ACGT 4 cut(s) 448, 776, 1598, 1606
HpySE526I ACGT 4 cut(s) 448, 776, 1598, 1606
Hsp92I GRCGYC 1 cut(s) 776
HspAI GCGC 4 cut(s) 73, 1262, 1620, 1810
KpnI GGTACC 1 cut(s) 722
KroI GCCGGC 1 cut(s) 3501
KroNI GCCGGC 1 cut(s) 3503
Ksp22I TGATCA 2 cut(s) 2743, 2848
KspAI GTTAAC 2 cut(s) 1524, 2694
LguI GCTCTTC 1 cut(s) 753
LmnI GCTCC 8 cut(s) 1573, 2435, 2813, 3007, 3289, 3410, 3515, 3791
MabI ACCWGGT 1 cut(s) 3064
MaeII ACGT 4 cut(s) 448, 776, 1598, 1606
MfeI CAATTG 3 cut(s) 318, 1500, 2241
MflI RGATCY 6 cut(s) 165, 649, 1163, 2152, 3454, 3662
MhlI GDGCHC 5 cut(s) 1096, 2566, 3046, 3487, 3529
MlsI TGGCCA 2 cut(s) 594, 742
MluNI TGGCCA 2 cut(s) 594, 742
MlyI GAGTC 5 cut(s) 685, 1400, 1524, 2621, 3881
MmeI TCCRAC 3 cut(s) 384, 3267, 3783
Mox20I TGGCCA 2 cut(s) 594, 742
Mph1103I ATGCAT 3 cut(s) 807, 1756, 2889
MroNI GCCGGC 1 cut(s) 3501
MroXI GAANNNNTTC 3 cut(s) 309, 1191, 1222
MscI TGGCCA 2 cut(s) 594, 742
MslI CAYNNNNRTG 2 cut(s) 1257, 1310
Msp20I TGGCCA 2 cut(s) 594, 742
MspA1I CMGCKG 5 cut(s) 1241, 1990, 2653, 3039, 3731
MspCI CTTAAG 2 cut(s) 1022, 1798
MspI CCGG 4 cut(s) 653, 1389, 2028, 3502
MunI CAATTG 3 cut(s) 318, 1500, 2241
Mva1269I GAATGC 7 cut(s) 355, 805, 1904, 1983, 2218, 3004, 3625
MvnI CGCG 1 cut(s) 1620
NaeI GCCGGC 1 cut(s) 3503
NciI CCSGG 1 cut(s) 2028
NgoMIV GCCGGC 1 cut(s) 3501
NheI GCTAGC 1 cut(s) 1979
NmuCI GTSAC 8 cut(s) 241, 377, 1894, 2795, 2986, 3242, 3635, 3873
NsbI TGCGCA 1 cut(s) 1811
NsiI ATGCAT 3 cut(s) 807, 1756, 2889
NspI RCATGY 5 cut(s) 1554, 1632, 1728, 2137, 3643
PagI TCATGA 2 cut(s) 298, 781
PceI AGGCCT 1 cut(s) 3214
PciI ACATGT 2 cut(s) 1628, 3639
PciSI GCTCTTC 1 cut(s) 753
PcsI WCGNNNNNNNCGW 1 cut(s) 2783
PctI GAATGC 7 cut(s) 355, 805, 1904, 1983, 2218, 3004, 3625
PdiI GCCGGC 1 cut(s) 3503
PdmI GAANNNNTTC 3 cut(s) 309, 1191, 1222
PflMI CCANNNNNTGG 2 cut(s) 1054, 3253
PfoI TCCNGGA 1 cut(s) 3004
PleI GAGTC 5 cut(s) 684, 1400, 1524, 2620, 3880
PmaCI CACGTG 1 cut(s) 449
PmlI CACGTG 1 cut(s) 449
PpsI GAGTC 5 cut(s) 684, 1400, 1524, 2620, 3880
Ppu21I YACGTR 1 cut(s) 449
PpuMI RGGWCCY 2 cut(s) 2543, 2633
PscI ACATGT 2 cut(s) 1628, 3639
PshBI ATTAAT 2 cut(s) 1859, 3405
Psp1406I AACGTT 1 cut(s) 1598
Psp5II RGGWCCY 2 cut(s) 2543, 2633
PspCI CACGTG 1 cut(s) 449
PspEI GGTNACC 1 cut(s) 3242
PspOMI GGGCCC 1 cut(s) 3042
PspPI GGNCC 6 cut(s) 1540, 2543, 2633, 2915, 3042, 3043
PspPPI RGGWCCY 2 cut(s) 2543, 2633
PstI CTGCAG 5 cut(s) 823, 1672, 1894, 3730, 3932
PstNI CAGNNNCTG 4 cut(s) 1422, 1571, 2836, 3023
PsuI RGATCY 6 cut(s) 165, 649, 1163, 2152, 3454, 3662
PvuII CAGCTG 4 cut(s) 1241, 1990, 2653, 3731
RseI CAYNNNNRTG 2 cut(s) 1257, 1310
SalI GTCGAC 1 cut(s) 2776
SapI GCTCTTC 1 cut(s) 753
Sau96I GGNCC 6 cut(s) 1540, 2543, 2633, 2915, 3042, 3043
ScaI AGTACT 1 cut(s) 1488
SchI GAGTC 5 cut(s) 685, 1400, 1524, 2621, 3881
SduI GDGCHC 5 cut(s) 1096, 2566, 3046, 3487, 3529
SexAI ACCWGGT 1 cut(s) 3064
SfcI CTRYAG 8 cut(s) 819, 909, 1515, 1668, 1890, 2763, 3726, 3928
SinI GGWCC 2 cut(s) 2543, 2633
SmiMI CAYNNNNRTG 2 cut(s) 1257, 1310
SmlI CTYRAG 3 cut(s) 1022, 1798, 2891
SmoI CTYRAG 3 cut(s) 1022, 1798, 2891
SpeI ACTAGT 1 cut(s) 517
SseBI AGGCCT 1 cut(s) 3214
SsiI CCGC 7 cut(s) 546, 1577, 3039, 3466, 3751, 3821, 3923
SspI AATATT 1 cut(s) 1765
StuI AGGCCT 1 cut(s) 3214
StyI CCWWGG 1 cut(s) 1159
TaiI ACGT 4 cut(s) 451, 779, 1601, 1609
TaqI TCGA 7 cut(s) 730, 2320, 2485, 2777, 3175, 3315, 3565
TatI WGTACW 5 cut(s) 501, 1486, 1769, 1924, 3418
TseFI GTSAC 8 cut(s) 241, 377, 1894, 2795, 2986, 3242, 3635, 3873
Tsp45I GTSAC 8 cut(s) 241, 377, 1894, 2795, 2986, 3242, 3635, 3873
TspGWI ACGGA 4 cut(s) 1928, 3411, 3601, 3932
Van91I CCANNNNNTGG 2 cut(s) 1054, 3253
Vha464I CTTAAG 2 cut(s) 1022, 1798
VneI GTGCAC 1 cut(s) 1092
VpaK11BI GGWCC 2 cut(s) 2543, 2633
VspI ATTAAT 2 cut(s) 1859, 3405
XagI CCTNNNNNAGG 2 cut(s) 2922, 3146
XceI RCATGY 5 cut(s) 1554, 1632, 1728, 2137, 3643
XcmI CCANNNNNNNNNTGG 1 cut(s) 2404
XmiI GTMKAC 5 cut(s) 60, 80, 1380, 2269, 2777
XmnI GAANNNNTTC 3 cut(s) 309, 1191, 1222
ZraI GACGTC 1 cut(s) 777
ZrmI AGTACT 1 cut(s) 1488
Zsp2I ATGCAT 3 cut(s) 807, 1756, 2889
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.