pycom01g24240

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0004502
Physical Location & Seq
Forward (+)
21210949 .. 21213530
2582 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g24240.2

Sequence Viewer

Length: 1746 bp
ATGCTAACAACATCTCCAAAGAAGCAGCTTTCTCGACTTTCTGTTCTTGCTCATCATTTTTCTTCTGATGAGAGCAAAAGTGCCTCCATTCAAGCTCATGCTGCATCGGCCATGCTAGCACTGGCTTCCATTTTGTCCCTTGAATCTGGCAGTTCTGTTGAGTCTTCTATCCTCGAGGTAGCAGTGCCTTTGATTCCTAGAAATGCCACACTATGTGAATACCTGAAGCTTTCATCAGGCAATGGAATTGAATTGCATTCGAATGGCCCTAGTGGTACTCTCTCATACTGGCATGGTCTAAGGGATGGATGTGTTGGCTTGCTGGAGTCTAGACTGAGGTGGGGAGGACCCTTAGCTGTTAAACAGCTGTGTGCAAGCAATATCCCTCTGCTTCTAGTGGATTTGTTAGCCAACAACCAACCAGAAGTTGGTAGCACCAATGATCAAGTTGGGCTTACCCCTATAGGAGTCATATGGACAATATCGTCAATATGTCACTGCCTTTCAGGTGGAGCCTTAACTTTTCGTCAGATTCTGCTTAGATGTAATCATATCAAGCTCATCTCTGACTTGATAGCTGATATGCATCTCAAGCTTGTAAAGTCTTGGGTTGGGCCTGGTGGAGGGAAAGATGGTGTAAGAGATATAACAAATGCAGTAATTGATGTCTTGGCATTTCCTTTTGTGGCCGTACAGAATGCTCCAGAATTGCCATCAGCCACTGCTTCAGTTAATAGCGGGGCCCTTCTCAACCTGGGTTCACCAGGGCTGAGAGTTGGCATGGAAGACAAAGACATGGTGAAAGTAATTGAAGAAGACCTTGGGAAGTATATTAAAAACCTTTTTGAGGTAGGAGTGCCAGGCATCGTTGTTTGGTGTTTAGGACACTTGGATTTAAAGGACATGGGAAGACCGGTGGCCTTTCTTGCAAAAATGGTTGGTTACCAACCATTGGCATTTCAAATTGTGGGTAAAGGCTTGCTGGATCCTAAAAGGATGAGAAGATTGCTGGACCGTTCTAGTCCAAGAGAGGTGATGCTGGATGTTCTGATGATTGTTTCTGATTTGGCTCGCATGGACAAGGGATTCTACAAATACATTAATGGAGCATCTGTATTGGAGTTCTTCAAGGAATTTCTCACCCATGAAGATCCTAATGTGCGCTCCAAGGCTTGCAGTGCTCTAGGAAATATGTGCCGGCACAGCTCCTACTTTTATAGTTCCCTGGCAAGGAATCAAATCATTGATCTCCTTATTGATCGATGTACTGATCCAGATAAACGGACACGAAAATTTGCTTGCTTTGCTATTGGCAATGCCGCCTACCATGACAAGATGTTATATGAAGAGCTACGAAGATCCATACCCTCTCTTGCGAATTTGTTGGTTTCGTCTGAAGAAGACAAGACTAAAGCAAATGCAGCAGGTGCACTAAGCAATCTTGTCCGCCACTCCGATCAGCTTTGGGAAGACATTGTATCCAAAGGAGCCATGCAGTCTTTACTGAAGTTGGTGGCTGATTGTTCGGTGGTAGCACTGAACCCGCCTAGTAGAAAAGATTCACTAAACGAGTCACCTCTTAAAATAGCTCTGATTGCATTGGCAAATATGTGTTCGCATCAGCCTTGCAGACAATTCCTCGGTTCATCACCGTTGTTCACTGTGATCGGACGGCTTCGGCAGTCTCCGGAATCAACAATTGCCAATCATGCCTCCCTGATATTCGCCAAAGTTGCTGATTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000166 GO:0000278 GO:0000280 GO:0000281 GO:0000910 GO:0000911 GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005856 GO:0005874 GO:0005881 GO:0006355 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006935 GO:0006996 GO:0007049 GO:0007112 GO:0007140 GO:0007154 GO:0007163 GO:0007165 GO:0007166 GO:0007224 GO:0007227 GO:0007228 GO:0007275 GO:0007276 GO:0007349 GO:0007584 GO:0008064 GO:0008092 GO:0008134 GO:0008144 GO:0008150 GO:0008152 GO:0008589 GO:0009524 GO:0009553 GO:0009558 GO:0009561 GO:0009605 GO:0009653 GO:0009791 GO:0009889 GO:0009966 GO:0009967 GO:0009987 GO:0009991 GO:0010033 GO:0010243 GO:0010468 GO:0010556 GO:0010639 GO:0010646 GO:0010647 GO:0014070 GO:0015630 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017076 GO:0019219 GO:0019222 GO:0019538 GO:0019894 GO:0019953 GO:0022402 GO:0022412 GO:0022414 GO:0022607 GO:0023051 GO:0023052 GO:0023056 GO:0030010 GO:0030030 GO:0030031 GO:0030554 GO:0030832 GO:0030833 GO:0030837 GO:0031156 GO:0031272 GO:0031323 GO:0031326 GO:0031333 GO:0031344 GO:0031667 GO:0032101 GO:0032104 GO:0032107 GO:0032271 GO:0032272 GO:0032501 GO:0032502 GO:0032504 GO:0032506 GO:0032535 GO:0032553 GO:0032555 GO:0032559 GO:0032956 GO:0032970 GO:0033043 GO:0033206 GO:0033273 GO:0035556 GO:0035639 GO:0036094 GO:0036211 GO:0040011 GO:0042221 GO:0042330 GO:0042493 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043254 GO:0043326 GO:0043327 GO:0043412 GO:0043900 GO:0044085 GO:0044087 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0044703 GO:0044782 GO:0045880 GO:0048229 GO:0048232 GO:0048285 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048609 GO:0048646 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051090 GO:0051091 GO:0051128 GO:0051129 GO:0051171 GO:0051252 GO:0051301 GO:0051321 GO:0051493 GO:0051494 GO:0051593 GO:0051704 GO:0051716 GO:0060176 GO:0060255 GO:0060271 GO:0060491 GO:0061640 GO:0065007 GO:0065008 GO:0065009 GO:0070925 GO:0071704 GO:0071840 GO:0080090 GO:0080134 GO:0080135 GO:0090066 GO:0097159 GO:0097367 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0110053 GO:0120031 GO:0120032 GO:0120035 GO:0120036 GO:0140013 GO:0140096 GO:1901265 GO:1901363 GO:1901564 GO:1901698 GO:1901700 GO:1902410 GO:1902903 GO:1902904 GO:1903046 GO:1903047 GO:1903506 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04341 map04341
Pfam Domains
Protein Families
Arm HEAT HEAT_2 Pkinase

Protein Analysis

582

Amino Acids

63.05

Weight (kDa)

8.44

Isoelectric Point (pI)

43.75

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1415
AasI GACNNNNNNGTC 1 cut(s) 484
AbsI CCTCGAGG 1 cut(s) 173
Acc36I ACCTGC 1 cut(s) 1415
AccB7I CCANNNNNTGG 2 cut(s) 428, 952
AccIII TCCGGA 1 cut(s) 1687
AciI CCGC 4 cut(s) 738, 1320, 1447, 1544
AclWI GGATC 5 cut(s) 980, 993, 1145, 1265, 1353
AcoI YGGCCR 2 cut(s) 108, 687
AcsI RAATTY 3 cut(s) 1133, 1292, 1378
AcuI CTGAAG 4 cut(s) 245, 711, 1416, 1526
AdeI CACNNNGTG 1 cut(s) 215
AfaI GTAC 3 cut(s) 277, 693, 1267
AfiI CCNNNNNNNGG 5 cut(s) 428, 623, 847, 952, 1230
AgeI ACCGGT 1 cut(s) 913
AgsI TTSAA 6 cut(s) 92, 143, 251, 812, 962, 1129
AjnI CCWGG 5 cut(s) 616, 753, 763, 859, 1224
AjuI GAANNNNNNNTTGG 2 cut(s) 804, 836
Alw21I GWGCWC 2 cut(s) 1183, 1432
Alw26I GTCTC 1 cut(s) 1689
Alw44I GTGCAC 1 cut(s) 1428
AlwI GGATC 5 cut(s) 980, 993, 1145, 1265, 1353
AlwNI CAGNNNCTG 2 cut(s) 535, 722
Ama87I CYCGRG 1 cut(s) 173
Aor13HI TCCGGA 1 cut(s) 1687
AoxI GGCC 6 cut(s) 108, 265, 614, 687, 741, 918
ApaI GGGCCC 1 cut(s) 745
ApaLI GTGCAC 1 cut(s) 1428
ApeKI GCWGC 3 cut(s) 25, 101, 1421
ApoI RAATTY 3 cut(s) 1133, 1292, 1378
AseI ATTAAT 1 cut(s) 1101
AsiGI ACCGGT 1 cut(s) 913
Asp700I GAANNNNTTC 1 cut(s) 1558
AspLEI GCGC 1 cut(s) 1164
AspS9I GGNCC 6 cut(s) 266, 347, 614, 741, 742, 1012
AsuHPI GGTGA 6 cut(s) 753, 811, 1045, 1132, 1566, 1641
AsuII TTCGAA 1 cut(s) 260
AsuNHI GCTAGC 1 cut(s) 115
AvaI CYCGRG 1 cut(s) 173
AvaII GGWCC 2 cut(s) 347, 1012
BaeGI GKGCMC 2 cut(s) 745, 1432
BamHI GGATCC 1 cut(s) 985
BanII GRGCYC 1 cut(s) 745
BbsI GAAGAC 6 cut(s) 156, 792, 822, 916, 1407, 1476
Bbv12I GWGCWC 2 cut(s) 1183, 1432
BbvI GCAGC 3 cut(s) 37, 88, 1433
BccI CCATC 3 cut(s) 299, 626, 721
BceAI ACGGC 2 cut(s) 674, 1688
BcgI CGANNNNNNTGC 2 cut(s) 14, 48
BciT130I CCWGG 5 cut(s) 618, 755, 765, 861, 1226
BciVI GTATCC 1 cut(s) 1489
BclI TGATCA 1 cut(s) 442
BcoDI GTCTC 1 cut(s) 1689
BfaI CTAG 8 cut(s) 116, 198, 270, 330, 395, 1020, 1184, 1548
BfmI CTRYAG 1 cut(s) 462
BfuAI ACCTGC 1 cut(s) 1415
BfuI GTATCC 1 cut(s) 1489
BisI GCNGC 4 cut(s) 26, 102, 1320, 1422
BlsI GCNGC 4 cut(s) 27, 103, 1321, 1423
Bme1390I CCNGG 5 cut(s) 618, 755, 765, 861, 1226
Bme18I GGWCC 2 cut(s) 347, 1012
BmeT110I CYCGRG 1 cut(s) 173
BmgT120I GGNCC 6 cut(s) 266, 347, 614, 741, 742, 1012
BmiI GGNNCC 6 cut(s) 349, 514, 742, 743, 987, 1489
BmrFI CCNGG 5 cut(s) 618, 755, 765, 861, 1226
BmsI GCATC 6 cut(s) 113, 595, 873, 1026, 1118, 1627
BmtI GCTAGC 1 cut(s) 119
BpiI GAAGAC 6 cut(s) 156, 792, 822, 916, 1407, 1476
BpmI CTGGAG 2 cut(s) 344, 687
Bpu10I CCTNAGC 1 cut(s) 352
Bpu14I TTCGAA 1 cut(s) 260
BpuEI CTTGAG 1 cut(s) 575
Bsa29I ATCGAT 1 cut(s) 1261
BsaBI GATNNNNATC 1 cut(s) 585
BsaJI CCNNGG 6 cut(s) 754, 764, 820, 1167, 1224, 1639
BsaWI WCCGGW 2 cut(s) 913, 1687
BsaXI ACNNNNNCTCC 3 cut(s) 28, 1098, 1128
Bsc4I CCNNNNNNNGG 5 cut(s) 428, 623, 847, 952, 1230
Bse118I RCCGGY 2 cut(s) 913, 1197
Bse1I ACTGG 2 cut(s) 126, 293
Bse3DI GCAATG 2 cut(s) 247, 1321
Bse8I GATNNNNATC 1 cut(s) 585
BseAI TCCGGA 1 cut(s) 1687
BseBI CCWGG 5 cut(s) 618, 755, 765, 861, 1226
BseCI ATCGAT 1 cut(s) 1261
BseDI CCNNGG 6 cut(s) 754, 764, 820, 1167, 1224, 1639
BseGI GGATG 4 cut(s) 310, 314, 1002, 1048
BseJI GATNNNNATC 1 cut(s) 585
BseLI CCNNNNNNNGG 5 cut(s) 428, 623, 847, 952, 1230
BseMI GCAATG 2 cut(s) 247, 1321
BseMII CTCAG 2 cut(s) 326, 761
BseNI ACTGG 2 cut(s) 126, 293
BseSI GKGCMC 2 cut(s) 745, 1432
BseXI GCAGC 3 cut(s) 37, 88, 1433
BshFI GGCC 6 cut(s) 110, 267, 616, 689, 743, 920
BshTI ACCGGT 1 cut(s) 913
BshVI ATCGAT 1 cut(s) 1261
BsiHKAI GWGCWC 2 cut(s) 1183, 1432
BsiHKCI CYCGRG 1 cut(s) 173
BsiSI CCGG 3 cut(s) 914, 1198, 1688
BslFI GGGAC 1 cut(s) 121
BslI CCNNNNNNNGG 5 cut(s) 428, 623, 847, 952, 1230
BsmAI GTCTC 1 cut(s) 1689
BsmFI GGGAC 1 cut(s) 121
BsmI GAATGC 2 cut(s) 256, 703
BsnI GGCC 6 cut(s) 110, 267, 616, 689, 743, 920
BsoBI CYCGRG 1 cut(s) 173
Bsp119I TTCGAA 1 cut(s) 260
Bsp120I GGGCCC 1 cut(s) 741
Bsp1286I GDGCHC 3 cut(s) 745, 1183, 1432
Bsp13I TCCGGA 1 cut(s) 1687
Bsp143I GATC 9 cut(s) 442, 985, 1150, 1246, 1258, 1270, 1358, 1456, 1665
BspACI CCGC 4 cut(s) 738, 1320, 1447, 1544
BspANI GGCC 6 cut(s) 110, 267, 616, 689, 743, 920
BspCNI CTCAG 2 cut(s) 327, 762
BspDI ATCGAT 1 cut(s) 1261
BspEI TCCGGA 1 cut(s) 1687
BspLI GGNNCC 6 cut(s) 349, 514, 742, 743, 987, 1489
BspMI ACCTGC 1 cut(s) 1415
BspOI GCTAGC 1 cut(s) 119
BspPI GGATC 5 cut(s) 980, 993, 1145, 1265, 1353
BspQI GCTCTTC 1 cut(s) 1341
BspT104I TTCGAA 1 cut(s) 260
BsrDI GCAATG 2 cut(s) 247, 1321
BsrFI RCCGGY 2 cut(s) 913, 1197
BsrI ACTGG 2 cut(s) 126, 293
BssAI RCCGGY 2 cut(s) 913, 1197
BssECI CCNNGG 6 cut(s) 754, 764, 820, 1167, 1224, 1639
BssMI GATC 9 cut(s) 442, 985, 1150, 1246, 1258, 1270, 1358, 1456, 1665
BssT1I CCWWGG 2 cut(s) 820, 1167
Bst2UI CCWGG 5 cut(s) 618, 755, 765, 861, 1226
Bst4CI ACNGT 3 cut(s) 1016, 1653, 1663
Bst6I CTCTTC 1 cut(s) 1341
BstAPI GCANNNNNTGC 1 cut(s) 1427
BstBI TTCGAA 1 cut(s) 260
BstC8I GCNNGC 8 cut(s) 117, 320, 376, 980, 1072, 1174, 1199, 1300
BstDEI CTNAG 6 cut(s) 299, 335, 352, 539, 770, 1433
BstEII GGTNACC 1 cut(s) 941
BstENI CCTNNNNNAGG 2 cut(s) 621, 845
BstF5I GGATG 4 cut(s) 310, 314, 1002, 1048
BstHHI GCGC 1 cut(s) 1164
BstKTI GATC 9 cut(s) 445, 988, 1153, 1249, 1261, 1273, 1361, 1459, 1668
BstMAI GTCTC 1 cut(s) 1689
BstMBI GATC 9 cut(s) 442, 985, 1150, 1246, 1258, 1270, 1358, 1456, 1665
BstNI CCWGG 5 cut(s) 618, 755, 765, 861, 1226
BstPI GGTNACC 1 cut(s) 941
BstSCI CCNGG 5 cut(s) 616, 753, 763, 859, 1224
BstSFI CTRYAG 1 cut(s) 462
BstSLI GKGCMC 2 cut(s) 745, 1432
BstV1I GCAGC 3 cut(s) 37, 88, 1433
BstV2I GAAGAC 6 cut(s) 156, 792, 822, 916, 1407, 1476
BstX2I RGATCY 3 cut(s) 985, 1150, 1358
BstYI RGATCY 3 cut(s) 985, 1150, 1358
Bsu15I ATCGAT 1 cut(s) 1261
BsuI GTATCC 1 cut(s) 1489
BsuRI GGCC 6 cut(s) 110, 267, 616, 689, 743, 920
BsuTUI ATCGAT 1 cut(s) 1261
BtsCI GGATG 4 cut(s) 310, 314, 1002, 1048
BtsI GCAGTG 4 cut(s) 189, 496, 720, 1183
BtsIMutI CAGTG 7 cut(s) 119, 189, 496, 720, 1183, 1535, 1659
BveI ACCTGC 1 cut(s) 1415
Cac8I GCNNGC 8 cut(s) 117, 320, 376, 980, 1072, 1174, 1199, 1300
CaiI CAGNNNCTG 2 cut(s) 535, 722
CfoI GCGC 1 cut(s) 1164
Cfr10I RCCGGY 2 cut(s) 913, 1197
Cfr13I GGNCC 6 cut(s) 266, 347, 614, 741, 742, 1012
ClaI ATCGAT 1 cut(s) 1261
Csp6I GTAC 3 cut(s) 276, 692, 1266
CspAI ACCGGT 1 cut(s) 913
CviQI GTAC 3 cut(s) 276, 692, 1266
DdeI CTNAG 6 cut(s) 299, 335, 352, 539, 770, 1433
DpnI GATC 9 cut(s) 444, 987, 1152, 1248, 1260, 1272, 1360, 1458, 1667
DpnII GATC 9 cut(s) 442, 985, 1150, 1246, 1258, 1270, 1358, 1456, 1665
DraI TTTAAA 1 cut(s) 897
DraIII CACNNNGTG 1 cut(s) 215
DrdI GACNNNNNNGTC 1 cut(s) 484
DseDI GACNNNNNNGTC 1 cut(s) 484
EaeI YGGCCR 2 cut(s) 108, 687
Eam1104I CTCTTC 1 cut(s) 1341
EarI CTCTTC 1 cut(s) 1341
EciI GGCGGA 1 cut(s) 1436
Eco130I CCWWGG 2 cut(s) 820, 1167
Eco24I GRGCYC 1 cut(s) 745
Eco47I GGWCC 2 cut(s) 347, 1012
Eco57I CTGAAG 4 cut(s) 245, 711, 1416, 1526
Eco88I CYCGRG 1 cut(s) 173
Eco91I GGTNACC 1 cut(s) 941
EcoNI CCTNNNNNAGG 2 cut(s) 621, 845
EcoO109I RGGNCCY 3 cut(s) 347, 741, 742
EcoO65I GGTNACC 1 cut(s) 941
EcoRII CCWGG 5 cut(s) 616, 753, 763, 859, 1224
EcoT14I CCWWGG 2 cut(s) 820, 1167
EcoT22I ATGCAT 1 cut(s) 588
EcoT38I GRGCYC 1 cut(s) 745
ErhI CCWWGG 2 cut(s) 820, 1167
FalI AAGNNNNNCTT 4 cut(s) 438, 470, 804, 836
FaqI GGGAC 1 cut(s) 121
FauI CCCGC 2 cut(s) 731, 1551
FauNDI CATATG 1 cut(s) 473
FbaI TGATCA 1 cut(s) 442
Fnu4HI GCNGC 4 cut(s) 26, 102, 1320, 1422
FokI GGATG 4 cut(s) 317, 321, 1009, 1055
FriOI GRGCYC 1 cut(s) 745
Fsp4HI GCNGC 4 cut(s) 26, 102, 1320, 1422
FspBI CTAG 8 cut(s) 116, 198, 270, 330, 395, 1020, 1184, 1548
GlaI GCGC 1 cut(s) 1163
GluI GCNGC 4 cut(s) 26, 102, 1320, 1422
GsuI CTGGAG 2 cut(s) 344, 687
HaeIII GGCC 6 cut(s) 110, 267, 616, 689, 743, 920
HapII CCGG 3 cut(s) 914, 1198, 1688
HhaI GCGC 1 cut(s) 1164
Hin6I GCGC 1 cut(s) 1162
HinP1I GCGC 1 cut(s) 1162
HindIII AAGCTT 2 cut(s) 227, 593
HpaII CCGG 3 cut(s) 914, 1198, 1688
HphI GGTGA 6 cut(s) 753, 811, 1045, 1132, 1566, 1641
Hpy166II GTNNAC 3 cut(s) 761, 1430, 1659
Hpy188I TCNGA 9 cut(s) 67, 531, 568, 1050, 1063, 1396, 1456, 1593, 1670
Hpy188III TCNNGA 6 cut(s) 33, 330, 704, 1274, 1688, 1743
Hpy8I GTNNAC 3 cut(s) 761, 1430, 1659
HpyAV CCTTC 1 cut(s) 755
HpyCH4III ACNGT 3 cut(s) 1016, 1653, 1663
HpyF3I CTNAG 6 cut(s) 299, 335, 352, 539, 770, 1433
HspAI GCGC 1 cut(s) 1162
Kpn2I TCCGGA 1 cut(s) 1687
KroI GCCGGC 1 cut(s) 1197
KroNI GCCGGC 1 cut(s) 1199
Ksp22I TGATCA 1 cut(s) 442
Kzo9I GATC 9 cut(s) 442, 985, 1150, 1246, 1258, 1270, 1358, 1456, 1665
LguI GCTCTTC 1 cut(s) 1341
LmnI GCTCC 6 cut(s) 512, 706, 1106, 1169, 1211, 1487
Lsp1109I GCAGC 3 cut(s) 37, 88, 1433
LweI GCATC 6 cut(s) 113, 595, 873, 1026, 1118, 1627
MaeI CTAG 8 cut(s) 116, 198, 270, 330, 395, 1020, 1184, 1548
MaeIII GTNAC 3 cut(s) 494, 941, 1572
MalI GATC 9 cut(s) 444, 987, 1152, 1248, 1260, 1272, 1360, 1458, 1667
MboI GATC 9 cut(s) 442, 985, 1150, 1246, 1258, 1270, 1358, 1456, 1665
MfeI CAATTG 1 cut(s) 1698
MflI RGATCY 3 cut(s) 985, 1150, 1358
MhlI GDGCHC 3 cut(s) 745, 1183, 1432
MlyI GAGTC 4 cut(s) 170, 335, 477, 1580
Mph1103I ATGCAT 1 cut(s) 588
MroI TCCGGA 1 cut(s) 1687
MroNI GCCGGC 1 cut(s) 1197
MroXI GAANNNNTTC 1 cut(s) 1558
MseI TTAA 7 cut(s) 360, 518, 732, 834, 896, 1101, 1581
MslI CAYNNNNRTG 1 cut(s) 261
MspA1I CMGCKG 1 cut(s) 367
MspI CCGG 3 cut(s) 914, 1198, 1688
MspR9I CCNGG 5 cut(s) 618, 755, 765, 861, 1226
MunI CAATTG 1 cut(s) 1698
Mva1269I GAATGC 2 cut(s) 256, 703
MvaI CCWGG 5 cut(s) 618, 755, 765, 861, 1226
NaeI GCCGGC 1 cut(s) 1199
NdeI CATATG 1 cut(s) 473
NdeII GATC 9 cut(s) 442, 985, 1150, 1246, 1258, 1270, 1358, 1456, 1665
NgoMIV GCCGGC 1 cut(s) 1197
NheI GCTAGC 1 cut(s) 115
NlaIV GGNNCC 6 cut(s) 349, 514, 742, 743, 987, 1489
NmuCI GTSAC 2 cut(s) 494, 1572
NsiI ATGCAT 1 cut(s) 588
NspV TTCGAA 1 cut(s) 260
PaeR7I CTCGAG 1 cut(s) 173
PaqCI CACCTGC 1 cut(s) 1415
PciSI GCTCTTC 1 cut(s) 1341
PctI GAATGC 2 cut(s) 256, 703
PdiI GCCGGC 1 cut(s) 1199
PdmI GAANNNNTTC 1 cut(s) 1558
PfeI GAWTC 8 cut(s) 143, 193, 532, 1086, 1234, 1559, 1691, 1739
PflMI CCANNNNNTGG 2 cut(s) 428, 952
PinAI ACCGGT 1 cut(s) 913
PkrI GCNGC 4 cut(s) 27, 103, 1321, 1423
PleI GAGTC 4 cut(s) 169, 334, 476, 1579
PpsI GAGTC 4 cut(s) 169, 334, 476, 1579
PpuMI RGGWCCY 1 cut(s) 347
PshBI ATTAAT 1 cut(s) 1101
Psp5II RGGWCCY 1 cut(s) 347
Psp6I CCWGG 5 cut(s) 616, 753, 763, 859, 1224
PspEI GGTNACC 1 cut(s) 941
PspGI CCWGG 5 cut(s) 616, 753, 763, 859, 1224
PspN4I GGNNCC 6 cut(s) 349, 514, 742, 743, 987, 1489
PspOMI GGGCCC 1 cut(s) 741
PspPI GGNCC 6 cut(s) 266, 347, 614, 741, 742, 1012
PspPPI RGGWCCY 1 cut(s) 347
PspXI VCTCGAGB 1 cut(s) 173
PstNI CAGNNNCTG 2 cut(s) 535, 722
PsuI RGATCY 3 cut(s) 985, 1150, 1358
PvuII CAGCTG 1 cut(s) 367
RsaI GTAC 3 cut(s) 277, 693, 1267
RsaNI GTAC 3 cut(s) 276, 692, 1266
RseI CAYNNNNRTG 1 cut(s) 261
SapI GCTCTTC 1 cut(s) 1341
SaqAI TTAA 7 cut(s) 360, 518, 732, 834, 896, 1101, 1581
SatI GCNGC 4 cut(s) 26, 102, 1320, 1422
Sau3AI GATC 9 cut(s) 442, 985, 1150, 1246, 1258, 1270, 1358, 1456, 1665
Sau96I GGNCC 6 cut(s) 266, 347, 614, 741, 742, 1012
SchI GAGTC 4 cut(s) 170, 335, 477, 1580
ScrFI CCNGG 5 cut(s) 618, 755, 765, 861, 1226
SduI GDGCHC 3 cut(s) 745, 1183, 1432
SfaNI GCATC 6 cut(s) 113, 595, 873, 1026, 1118, 1627
SfcI CTRYAG 1 cut(s) 462
Sfr274I CTCGAG 1 cut(s) 173
SfuI TTCGAA 1 cut(s) 260
SinI GGWCC 2 cut(s) 347, 1012
SlaI CTCGAG 1 cut(s) 173
SmiMI CAYNNNNRTG 1 cut(s) 261
SmlI CTYRAG 2 cut(s) 173, 590
SmoI CTYRAG 2 cut(s) 173, 590
SsiI CCGC 4 cut(s) 738, 1320, 1447, 1544
SspMI CTAG 8 cut(s) 116, 198, 270, 330, 395, 1020, 1184, 1548
StyD4I CCNGG 5 cut(s) 616, 753, 763, 859, 1224
StyI CCWWGG 2 cut(s) 820, 1167
TaaI ACNGT 3 cut(s) 1016, 1653, 1663
TaqI TCGA 4 cut(s) 34, 174, 260, 1261
TatI WGTACW 1 cut(s) 1265
TauI GCSGC 1 cut(s) 1322
TfiI GAWTC 8 cut(s) 143, 193, 532, 1086, 1234, 1559, 1691, 1739
Tru1I TTAA 7 cut(s) 360, 518, 732, 834, 896, 1101, 1581
Tru9I TTAA 7 cut(s) 360, 518, 732, 834, 896, 1101, 1581
TscAI CASTG 7 cut(s) 126, 189, 503, 727, 1183, 1542, 1666
TseFI GTSAC 2 cut(s) 494, 1572
TseI GCWGC 3 cut(s) 25, 101, 1421
Tsp45I GTSAC 2 cut(s) 494, 1572
TspDTI ATGAA 4 cut(s) 222, 1161, 1359, 1635
TspGWI ACGGA 1 cut(s) 1297
TspRI CASTG 7 cut(s) 126, 189, 503, 727, 1183, 1542, 1666
Van91I CCANNNNNTGG 2 cut(s) 428, 952
VneI GTGCAC 1 cut(s) 1428
VpaK11BI GGWCC 2 cut(s) 347, 1012
VspI ATTAAT 1 cut(s) 1101
XagI CCTNNNNNAGG 2 cut(s) 621, 845
XapI RAATTY 3 cut(s) 1133, 1292, 1378
XbaI TCTAGA 1 cut(s) 329
XcmI CCANNNNNNNNNTGG 2 cut(s) 118, 425
XhoI CTCGAG 1 cut(s) 173
XmnI GAANNNNTTC 1 cut(s) 1558
XspI CTAG 8 cut(s) 116, 198, 270, 330, 395, 1020, 1184, 1548
Zsp2I ATGCAT 1 cut(s) 588
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.