FvH4_7g33760

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0004502
Physical Location & Seq
Forward (+)
23933550 .. 23943029
9480 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g33760.t3

Sequence Viewer

Length: 4041 bp
ATGGGTGTCGAGAATTATCATGTAATTGAGCTTGTAGGTGAAGGTTCCTTCGGGAAGGTATACAAGGGAAGACGCAAGTTCACAGGCCAGACTGTTGCGATGAAATTCATTATGAAACATGGGAAAAGTGACAAGGATATCCACAATTTAAGACAAGAAATTGAGATTCTAAGGAAGCTGAAGCATGAAAATATCATTGAAATGCTTGATTCCTTTGAAAGCCCACAAGAGTTTTGTGTTGTCACAGAGTTTGCACAAGGTGAACTATTTGAAATTCTTGAGGATGATAAGCACCTTCCAGAAGAACAAGTTCAAGCAATTGCTAAGCAATTGGTGAGAGCATTGCATTATCTCCATTCAAACCGCATCATCCACCGTGACATGAAACCACAAAACATTCTCATTGGTGCTGGTTCTATTGTTAAGCTTTGTGACTTTGGGTTTGCACGAGCAATGTCCACAAATACTGTTGTTTTGCGATCCATAAAAGGTACTCCCCTGTACATGGCTCCTGAACTAGTGCGGGAACAACCTTACAACCACACTGCAGATTTATGGTCTCTCGGAGTTATATTATATGAATTATTTGTTGGCCAGCCTCCATTCTATACAAATTCAGTATATGCACTAGTTCGGCATATAGTGAAGGATCCTGTCAAATACCCTGACAACATGAGTTCAAGTTTTAAAAACTTTCTGAAGGGGTTGCTCAATAAGGTGCCTCAAAATCGTTTGACTTGGCCAGCTCTTTTGGAACACCCATTTGTTAAGGAGACAGCTGAAGAACTGGAAGCTAGGGAAATGCGGTCTGCTACTGCTGCAGAAAGGGGGTGCGTTGCAGCATGGAGGGGTGAGGGAAACAAAATCCAAACACCAGGTGGCTTGGCCGTTTCTAGTCCTGTAGGTATAGTCACATCTACAGCTTCTTCAGAGGACAACAGTGGAATAAGTTTTCAGGATGATGCTCAATCAAATATTCCAGACTCCAAAGCAGTCAATTCTTCTCCTAATGAGGAGTTTCCTGGGTTTGCAAATCCTGATGAAGTTAAACAGTCAGGTTGTCAAATTCTCGACAGACTGGAAAATAACTCGCGTACGGTTAAAGGTGCACTAATAATTGGTCAAGATAATGAAGCATTAGCACATGTTTTGCTACCCATAAAACGATGCTCGCAAGGATCCCAAAATTCCAGCAGGGATCAAGATATTCTTACTTCAAATCAATCCTTAAGAATCCTTTCAAACTTAGTTGCAGTTGGTGCCATCACTTCCAGTGGGCTGCTTGATGAAATCATACATGAAATTCTTGTTTATACCACCTTCATTGTGAGCATCAAATCTTCTGAACTGAATGAGTTAAGAGCAAAGAGTTTCTCAATAATCAAAGTTTTGGTAGACAATGTTGGACATGACATTGGAAGCTCATATTTCAGGCACTGGGTTGCTTTAGCTGAGATTTTTTCACAGGTTGTTGGTTGCAGTGAAGATGCATCTGGAAGAGTTATGCTTGAATCCATAGCTTGCATTACTGCCATGTTAACAAGAGTTAATGAGGGCCTTAAAGTGTTATTCTCAACTCCAGCTCGCCAGGAGGTTTGTGGTCCTAATGAAGCAGTGAAACAGATTTTGGATCATGCTAAAACATCTGGTTTAGCGGATCAGCTTTGTCTCTGCTTAGCCACTGCTGGTGCAAGTCTTATTTCAGGTTCTTCAAATATGTTGCGCTCAGCGTGTGAAGCATGCAGGGCTATTTGGTTATTGGTAGATGCATCAGAGTTTTTTTCTACAAAAGGAAATGTCGTTTCCTTTCCGCTGAACACTATGGCTAGTCCAGCAAGGGATCAAGATCGGGGAGGTTCTGTGATTTTGATAGAATCAGCAAAACTTGTAGCTGTAGTTACTAGAGCATTAGTTAGATCTAAACCAGTTCAGGTTGCCATCCATTATTGCCTTCATCAACGTCTTGAGGCTTCACTCTATGCTGGTATTCAGCTCTTGTTGAGGTGCTGCTTGCAGAGTGGAATTGTTCCTGGCATTCTCTGTGGCCTGCCTAGTTCCTTACCTGTAACTACTGTTGTCAGTGGTGGAGGTGATAGGACCATTATTTCAGAGATATTCTCTTTGTTGTCTTTATGCATTTCATCTCAAAATAAAGATCCACAAGCAATTGAGACAACCAACTTGAAGAGCAAAATAAGCGATCCCAATACATTGGTCATGCATTCCTGCCTCATCCTTGCATCAGTGGCACAATGTTTAAAGGCGACTGGAAGAAATTCTGCATTGTTTATGCTTACTACATCCTCGAAGAATCAGCTTTCTCGACTTTCTGTCCTTGCTCATCACTTTTCTTCTGGTGAGAGCACAAATACTTCCTCTCGAGCTCACAGTGCATCAGCCATGTTGGCACTGGCATCTATTTTGTCCCTTGAATCTGGAGCTTCTGTTGGTTCCTCTGTCTTTGAAGTAGCTGTGCCTTTGATTCCTCAAACCACCACACTTTGTGAATACCTCAAACTTCCATCAAACAGTGAGATTGTCTCAGGTCCTAATGGCACCAATGGTGTTCTCTCAAATTGGCATGGCCTTAGGGATGGATGTGTTGGCCTTCTGGAGGCGAGATTGAGGTGGGGAGGACCTTCAGCTGTCCAACAAATGTGTGCAAGCAATATCCCTCTGCTTCTAATCAATTTGTTAGCCAAAAACCAACAATATTCTTCTCCTGAAGAAGAATCCAGTGCAAGTGATCAAGTTGGGCTTTCCCCTATAGGAGTTGTGTGGACAGTATCATCTATTTGTCAGTGCCTTTCAGGTGGAGCTTTAACCTTTCGTCAGATTCTGCTTAGAAGTGATCATATCAAACTCTTCTCTGACTTGATATCCGATACGCATCTCAAACTTGTAAAGTCCTGGGCTGGGCCTGGTGGAGGAATGGATGGCGTAAGAGATATAACAAACGCAATAATTGACCTCTTGGCATTTCCTTTTGTGGCTGTTCAGAATGCTCCAGGATTGCCAGCTGCAACTGCCTCAGTTAATAGTGGGATCCTTCTAAATATGGGGTCACCAGGTGTTAAAGTCGGCATGGAAGACAGAGACATGGTGAAAGTGATTGAGGAGGACCTGGGAAAGTATATCAAGATCCTCTTGGAGGTAGGAGTGCCTGGTATCATTCTTTGGTGTCTAGAACATTTGGAGTTAAAAGATTTGGGAAGACCTGTGGCCTTTCTGGCTAAGATGATAGCTCAGCGACCATTGGCAGTTCAACTTGTGGGTAAAGGCTTGTTGGATCCTACTAAGATGAGAAGATTGCTGGATCGTTCAAGCCCAAAAGAAGTCATTCTGGATGGTCTGATGATTGTTTCTGATTTGGCTCGCATGGACAAGGGATTCTATGAATACATTAACCGGGCTTCTCTGCTGGAGTTCTTCAAGGGATTTCTTGTTCATGAAGATCCTAGTGTGCGTTCGAAGGCTTGCAGTGCTCTAGGCAACATGTGCCGGCACAGCTCCTATTTTTACAGTTCCCTTGCAAGGAATCAAATCATTGGCCTCCTTATTGATCGATGCTCTGACCCAGACAAACGTACACGGAAATTTGCTTGTTTTGCTATTGGAAATGCCGCTTACCATAACGATATGTTGTACGAAGAGCTGCGGCGATCTATCCCCAAGCTTGCCAATTTGTTGCTTTCATCTGAGGAAGACAAGACAAAAGCAAATGCAGCAGGTGCACTAAGCAATCTTATCCGCAACTCCAACAAGCTTTGCGAAGACATTGTATCCAAAGGAGCCATGCAGTCTTTGCTGAAGTTGGTGGCAGAGTGTTCAGAGCTAGCTCTGAATCCAAGTAGGAGAGATTCGGCGCATGAGTCACCTCTCAAAATAGCTCTGTTTTCATTGGCAAAGATGTGCTCACATCCGCCGTGCAGAGACTTCCTCCGTTCATCTGACTTGTTCCCTGTGATCGGACGGCTGCGGCAATCCCCAGAATCAACAATTGCCAACTACGCATCTGCCATTATCAACAAAGTTGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000166 GO:0000278 GO:0000280 GO:0000281 GO:0000910 GO:0000911 GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005856 GO:0005874 GO:0005881 GO:0006355 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006935 GO:0006996 GO:0007049 GO:0007112 GO:0007140 GO:0007154 GO:0007163 GO:0007165 GO:0007166 GO:0007224 GO:0007227 GO:0007228 GO:0007275 GO:0007276 GO:0007349 GO:0007584 GO:0008064 GO:0008092 GO:0008134 GO:0008144 GO:0008150 GO:0008152 GO:0008589 GO:0009524 GO:0009553 GO:0009558 GO:0009561 GO:0009605 GO:0009653 GO:0009791 GO:0009889 GO:0009966 GO:0009967 GO:0009987 GO:0009991 GO:0010033 GO:0010243 GO:0010468 GO:0010556 GO:0010639 GO:0010646 GO:0010647 GO:0014070 GO:0015630 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017076 GO:0019219 GO:0019222 GO:0019538 GO:0019894 GO:0019953 GO:0022402 GO:0022412 GO:0022414 GO:0022607 GO:0023051 GO:0023052 GO:0023056 GO:0030010 GO:0030030 GO:0030031 GO:0030554 GO:0030832 GO:0030833 GO:0030837 GO:0031156 GO:0031272 GO:0031323 GO:0031326 GO:0031333 GO:0031344 GO:0031667 GO:0032101 GO:0032104 GO:0032107 GO:0032271 GO:0032272 GO:0032501 GO:0032502 GO:0032504 GO:0032506 GO:0032535 GO:0032553 GO:0032555 GO:0032559 GO:0032956 GO:0032970 GO:0033043 GO:0033206 GO:0033273 GO:0035556 GO:0035639 GO:0036094 GO:0036211 GO:0040011 GO:0042221 GO:0042330 GO:0042493 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043254 GO:0043326 GO:0043327 GO:0043412 GO:0043900 GO:0044085 GO:0044087 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0044703 GO:0044782 GO:0045880 GO:0048229 GO:0048232 GO:0048285 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048609 GO:0048646 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051090 GO:0051091 GO:0051128 GO:0051129 GO:0051171 GO:0051252 GO:0051301 GO:0051321 GO:0051493 GO:0051494 GO:0051593 GO:0051704 GO:0051716 GO:0060176 GO:0060255 GO:0060271 GO:0060491 GO:0061640 GO:0065007 GO:0065008 GO:0065009 GO:0070925 GO:0071704 GO:0071840 GO:0080090 GO:0080134 GO:0080135 GO:0090066 GO:0097159 GO:0097367 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0110053 GO:0120031 GO:0120032 GO:0120035 GO:0120036 GO:0140013 GO:0140096 GO:1901265 GO:1901363 GO:1901564 GO:1901698 GO:1901700 GO:1902410 GO:1902903 GO:1902904 GO:1903046 GO:1903047 GO:1903506 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04341 map04341
Pfam Domains
Protein Families
Arm HEAT HEAT_2 Pkinase

Protein Analysis

1347

Amino Acids

146.41

Weight (kDa)

7.31

Isoelectric Point (pI)

49.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 27 - 195 8.9e-29 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 29 - 200 5.2e-52 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 3719
Acc36I ACCTGC 1 cut(s) 3719
AccB1I GGYRCC 3 cut(s) 718, 1259, 2555
AccI GTMKAC 2 cut(s) 60, 1395
AccII CGCG 1 cut(s) 1093
AcoI YGGCCR 3 cut(s) 592, 740, 885
AcsI RAATTY 8 cut(s) 104, 273, 613, 1065, 1186, 1302, 2275, 3596
AcuI CTGAAG 7 cut(s) 200, 719, 801, 912, 2625, 2745, 3830
AdeI CACNNNGTG 4 cut(s) 260, 878, 2504, 3071
AfaI GTAC 5 cut(s) 493, 503, 1096, 3589, 3647
AfiI CCNNNNNNNGG 7 cut(s) 505, 2614, 2916, 2927, 3151, 3534, 3968
AflII CTTAAG 1 cut(s) 1228
AflIII ACRYGT 2 cut(s) 1144, 3495
AhdI GACNNNNNGTC 1 cut(s) 2330
AhlI ACTAGT 2 cut(s) 517, 628
AjuI GAANNNNNNNTTGG 4 cut(s) 573, 605, 1610, 1642
AloI GAACNNNNNNTCC 2 cut(s) 3429, 3461
Alw21I GWGCWC 6 cut(s) 1111, 2366, 2386, 3487, 3736, 3917
Alw26I GTCTC 7 cut(s) 564, 767, 1673, 2165, 2545, 3090, 3927
Alw44I GTGCAC 2 cut(s) 1107, 3732
AlwNI CAGNNNCTG 2 cut(s) 1437, 2839
Ama87I CYCGRG 1 cut(s) 2379
ApaLI GTGCAC 2 cut(s) 1107, 3732
ApeKI GCWGC 8 cut(s) 818, 839, 1279, 2007, 3020, 3655, 3725, 3976
ApoI RAATTY 8 cut(s) 104, 273, 613, 1065, 1186, 1302, 2275, 3596
ArsI GACNNNNNNTTYG 2 cut(s) 425, 457
Asp700I GAANNNNTTC 4 cut(s) 309, 1237, 2275, 3339
AspLEI GCGC 2 cut(s) 1725, 3868
AspS9I GGNCC 7 cut(s) 1555, 1601, 2097, 2546, 2636, 2918, 3121
AsuC2I CCSGG 1 cut(s) 3410
AsuHPI GGTGA 9 cut(s) 50, 272, 346, 863, 2102, 2369, 3057, 3115, 3867
AsuII TTCGAA 1 cut(s) 3470
AsuNHI GCTAGC 1 cut(s) 3835
AvaI CYCGRG 1 cut(s) 2379
AvaII GGWCC 5 cut(s) 1601, 2097, 2546, 2636, 3121
AxyI CCTNAGG 1 cut(s) 2588
BaeGI GKGCMC 2 cut(s) 1111, 3736
BalI TGGCCA 2 cut(s) 594, 742
BamHI GGATCC 4 cut(s) 649, 1178, 3045, 3289
BanI GGYRCC 3 cut(s) 718, 1259, 2555
BanII GRGCYC 1 cut(s) 2386
BauI CACGAG 1 cut(s) 447
BbsI GAAGAC 5 cut(s) 76, 3096, 3220, 3711, 3780
Bbv12I GWGCWC 6 cut(s) 1111, 2366, 2386, 3487, 3736, 3917
BbvI GCAGC 8 cut(s) 805, 851, 1266, 1994, 3007, 3642, 3737, 3963
BccI CCATC 6 cut(s) 1271, 1946, 2530, 2588, 2930, 3341
BceAI ACGGC 3 cut(s) 872, 3910, 3989
BciVI GTATCC 1 cut(s) 3793
BclI TGATCA 2 cut(s) 2746, 2851
BcnI CCSGG 1 cut(s) 3410
BcoDI GTCTC 7 cut(s) 564, 767, 1673, 2165, 2545, 3090, 3927
BcuI ACTAGT 2 cut(s) 517, 628
BfmI CTRYAG 6 cut(s) 546, 819, 900, 918, 1893, 2766
BfrI CTTAAG 1 cut(s) 1228
BfuAI ACCTGC 1 cut(s) 3719
BfuI GTATCC 1 cut(s) 3793
BglI GCCNNNNNGGC 2 cut(s) 2405, 3230
BglII AGATCT 1 cut(s) 1916
BlpI GCTNAGC 4 cut(s) 324, 1675, 1726, 3246
Bme18I GGWCC 5 cut(s) 1601, 2097, 2546, 2636, 3121
BmeRI GACNNNNNGTC 1 cut(s) 2330
BmeT110I CYCGRG 1 cut(s) 2379
BmgT120I GGNCC 7 cut(s) 1555, 1601, 2097, 2546, 2636, 2918, 3121
BmrI ACTGGG 1 cut(s) 1447
BmtI GCTAGC 1 cut(s) 3839
BmuI ACTGGG 1 cut(s) 1447
BpiI GAAGAC 5 cut(s) 76, 3096, 3220, 3711, 3780
BplI GAGNNNNNCTC 6 cut(s) 2102, 2134, 2525, 2557, 3924, 3956
BpmI CTGGAG 5 cut(s) 1563, 2457, 2633, 2991, 3443
Bpu1102I GCTNAGC 4 cut(s) 324, 1675, 1726, 3246
Bpu14I TTCGAA 1 cut(s) 3470
BpuEI CTTGAG 2 cut(s) 299, 1985
BpuMI CCSGG 1 cut(s) 3410
Bsa29I ATCGAT 1 cut(s) 3565
BsaBI GATNNNNATC 2 cut(s) 1845, 2889
BsaI GGTCTC 1 cut(s) 564
BsaJI CCNNGG 3 cut(s) 1022, 2910, 3124
BsaXI ACNNNNNCTCC 4 cut(s) 1845, 1875, 2430, 2460
Bsc4I CCNNNNNNNGG 7 cut(s) 505, 2614, 2916, 2927, 3151, 3534, 3968
Bse118I RCCGGY 1 cut(s) 3501
Bse1I ACTGG 8 cut(s) 792, 1083, 1272, 1442, 1925, 2272, 2415, 2736
Bse21I CCTNAGG 1 cut(s) 2588
Bse3DI GCAATG 2 cut(s) 341, 459
Bse8I GATNNNNATC 2 cut(s) 1845, 2889
BseCI ATCGAT 1 cut(s) 3565
BseDI CCNNGG 3 cut(s) 1022, 2910, 3124
BseJI GATNNNNATC 2 cut(s) 1845, 2889
BseLI CCNNNNNNNGG 7 cut(s) 505, 2614, 2916, 2927, 3151, 3534, 3968
BseMI GCAATG 2 cut(s) 341, 459
BseMII CTCAG 6 cut(s) 1443, 1740, 2556, 3045, 3260, 3690
BseNI ACTGG 8 cut(s) 792, 1083, 1272, 1442, 1925, 2272, 2415, 2736
BseRI GAGGAG 2 cut(s) 1028, 3131
BseSI GKGCMC 2 cut(s) 1111, 3736
BseXI GCAGC 8 cut(s) 805, 851, 1266, 1994, 3007, 3642, 3737, 3963
BseYI CCCAGC 1 cut(s) 2915
BsgI GTGCAG 1 cut(s) 3949
Bsh1236I CGCG 1 cut(s) 1093
BshNI GGYRCC 3 cut(s) 718, 1259, 2555
BshVI ATCGAT 1 cut(s) 3565
BsiHKAI GWGCWC 6 cut(s) 1111, 2366, 2386, 3487, 3736, 3917
BsiHKCI CYCGRG 1 cut(s) 2379
BsiSI CCGG 2 cut(s) 3409, 3502
BsiWI CGTACG 1 cut(s) 1094
BslFI GGGAC 1 cut(s) 2410
BslI CCNNNNNNNGG 7 cut(s) 505, 2614, 2916, 2927, 3151, 3534, 3968
BsmAI GTCTC 7 cut(s) 564, 767, 1673, 2165, 2545, 3090, 3927
BsmFI GGGAC 1 cut(s) 2410
BsmI GAATGC 3 cut(s) 2034, 2221, 3007
Bso31I GGTCTC 1 cut(s) 564
BsoBI CYCGRG 1 cut(s) 2379
Bsp119I TTCGAA 1 cut(s) 3470
Bsp1286I GDGCHC 6 cut(s) 1111, 2366, 2386, 3487, 3736, 3917
Bsp1407I TGTACA 1 cut(s) 501
Bsp1720I GCTNAGC 4 cut(s) 324, 1675, 1726, 3246
BspCNI CTCAG 6 cut(s) 1444, 1739, 2555, 3044, 3259, 3691
BspDI ATCGAT 1 cut(s) 3565
BspFNI CGCG 1 cut(s) 1093
BspHI TCATGA 1 cut(s) 3448
BspMAI CTGCAG 2 cut(s) 550, 823
BspMI ACCTGC 1 cut(s) 3719
BspOI GCTAGC 1 cut(s) 3839
BspQI GCTCTTC 2 cut(s) 2180, 3645
BspT104I TTCGAA 1 cut(s) 3470
BspT107I GGYRCC 3 cut(s) 718, 1259, 2555
BspTI CTTAAG 1 cut(s) 1228
BspTNI GGTCTC 1 cut(s) 564
BsrDI GCAATG 2 cut(s) 341, 459
BsrFI RCCGGY 1 cut(s) 3501
BsrGI TGTACA 1 cut(s) 501
BsrI ACTGG 8 cut(s) 792, 1083, 1272, 1442, 1925, 2272, 2415, 2736
BssAI RCCGGY 1 cut(s) 3501
BssECI CCNNGG 3 cut(s) 1022, 2910, 3124
BssNAI GTATAC 1 cut(s) 61
BssSI CACGAG 1 cut(s) 447
Bst1107I GTATAC 1 cut(s) 61
Bst2BI CACGAG 1 cut(s) 447
Bst6I CTCTTC 4 cut(s) 1492, 2180, 2870, 3645
BstAFI CTTAAG 1 cut(s) 1228
BstAPI GCANNNNNTGC 4 cut(s) 1259, 3498, 3731, 3805
BstAUI TGTACA 1 cut(s) 501
BstBI TTCGAA 1 cut(s) 3470
BstEII GGTNACC 1 cut(s) 3063
BstENI CCTNNNNNAGG 3 cut(s) 2612, 2925, 3149
BstFNI CGCG 1 cut(s) 1093
BstHHI GCGC 2 cut(s) 1725, 3868
BstMAI GTCTC 7 cut(s) 564, 767, 1673, 2165, 2545, 3090, 3927
BstNSI RCATGY 3 cut(s) 1148, 1743, 3499
BstPI GGTNACC 1 cut(s) 3063
BstSFI CTRYAG 6 cut(s) 546, 819, 900, 918, 1893, 2766
BstSLI GKGCMC 2 cut(s) 1111, 3736
BstUI CGCG 1 cut(s) 1093
BstV1I GCAGC 8 cut(s) 805, 851, 1266, 1994, 3007, 3642, 3737, 3963
BstV2I GAAGAC 5 cut(s) 76, 3096, 3220, 3711, 3780
BstX2I RGATCY 8 cut(s) 649, 1178, 1916, 2155, 3045, 3141, 3289, 3454
BstXI CCANNNNNNTGG 1 cut(s) 2212
BstYI RGATCY 8 cut(s) 649, 1178, 1916, 2155, 3045, 3141, 3289, 3454
BstZ17I GTATAC 1 cut(s) 61
Bsu15I ATCGAT 1 cut(s) 3565
Bsu36I CCTNAGG 1 cut(s) 2588
BsuI GTATCC 1 cut(s) 3793
BsuTUI ATCGAT 1 cut(s) 3565
BtgZI GCGATG 1 cut(s) 113
BtsI GCAGTG 5 cut(s) 543, 1486, 1620, 1680, 3487
BveI ACCTGC 1 cut(s) 3719
CaiI CAGNNNCTG 2 cut(s) 1437, 2839
CciI TCATGA 1 cut(s) 3448
CfoI GCGC 2 cut(s) 1725, 3868
Cfr10I RCCGGY 1 cut(s) 3501
Cfr13I GGNCC 7 cut(s) 1555, 1601, 2097, 2546, 2636, 2918, 3121
ClaI ATCGAT 1 cut(s) 3565
CseI GACGC 1 cut(s) 81
CsiI ACCWGGT 2 cut(s) 874, 3067
Csp6I GTAC 5 cut(s) 492, 502, 1095, 3588, 3646
CspCI CAANNNNNGTGG 2 cut(s) 1306, 1341
CviQI GTAC 5 cut(s) 492, 502, 1095, 3588, 3646
DraI TTTAAA 2 cut(s) 688, 2259
DraIII CACNNNGTG 4 cut(s) 260, 878, 2504, 3071
DriI GACNNNNNGTC 1 cut(s) 2330
EaeI YGGCCR 3 cut(s) 592, 740, 885
Eam1104I CTCTTC 4 cut(s) 1492, 2180, 2870, 3645
Eam1105I GACNNNNNGTC 1 cut(s) 2330
EarI CTCTTC 4 cut(s) 1492, 2180, 2870, 3645
EciI GGCGGA 1 cut(s) 3912
Ecl136II GAGCTC 1 cut(s) 2384
Eco24I GRGCYC 1 cut(s) 2386
Eco31I GGTCTC 1 cut(s) 564
Eco32I GATATC 2 cut(s) 139, 2880
Eco47I GGWCC 5 cut(s) 1601, 2097, 2546, 2636, 3121
Eco53kI GAGCTC 1 cut(s) 2384
Eco57I CTGAAG 7 cut(s) 200, 719, 801, 912, 2625, 2745, 3830
Eco81I CCTNAGG 1 cut(s) 2588
Eco88I CYCGRG 1 cut(s) 2379
Eco91I GGTNACC 1 cut(s) 3063
EcoICRI GAGCTC 1 cut(s) 2384
EcoNI CCTNNNNNAGG 3 cut(s) 2612, 2925, 3149
EcoO109I RGGNCCY 4 cut(s) 1555, 2546, 2636, 3121
EcoO65I GGTNACC 1 cut(s) 3063
EcoRV GATATC 2 cut(s) 139, 2880
EcoT22I ATGCAT 4 cut(s) 1492, 1771, 2138, 2223
EcoT38I GRGCYC 1 cut(s) 2386
FalI AAGNNNNNCTT 6 cut(s) 1194, 1226, 2742, 2774, 3131, 3163
FaqI GGGAC 1 cut(s) 2410
FauI CCCGC 1 cut(s) 516
FbaI TGATCA 2 cut(s) 2746, 2851
FblI GTMKAC 2 cut(s) 60, 1395
FriOI GRGCYC 1 cut(s) 2386
GlaI GCGC 2 cut(s) 1724, 3867
GsaI CCCAGC 1 cut(s) 2919
GsuI CTGGAG 5 cut(s) 1563, 2457, 2633, 2991, 3443
HapII CCGG 2 cut(s) 3409, 3502
HgaI GACGC 1 cut(s) 81
HhaI GCGC 2 cut(s) 1725, 3868
Hin6I GCGC 2 cut(s) 1723, 3866
HinP1I GCGC 2 cut(s) 1723, 3866
HincII GTYRAC 1 cut(s) 1539
HindII GTYRAC 1 cut(s) 1539
HindIII AAGCTT 3 cut(s) 425, 3674, 3764
HpaI GTTAAC 1 cut(s) 1539
HpaII CCGG 2 cut(s) 3409, 3502
HphI GGTGA 9 cut(s) 50, 272, 346, 863, 2102, 2369, 3057, 3115, 3867
HpyCH4IV ACGT 2 cut(s) 1960, 3586
HpySE526I ACGT 2 cut(s) 1960, 3586
HspAI GCGC 2 cut(s) 1723, 3866
KroI GCCGGC 1 cut(s) 3501
KroNI GCCGGC 1 cut(s) 3503
Ksp22I TGATCA 2 cut(s) 2746, 2851
KspAI GTTAAC 1 cut(s) 1539
LguI GCTCTTC 2 cut(s) 2180, 3645
LmnI GCTCC 6 cut(s) 514, 2438, 2816, 3010, 3515, 3791
Lsp1109I GCAGC 8 cut(s) 805, 851, 1266, 1994, 3007, 3642, 3737, 3963
MabI ACCWGGT 2 cut(s) 874, 3067
MaeII ACGT 2 cut(s) 1960, 3586
MaeIII GTNAC 9 cut(s) 128, 241, 377, 431, 910, 1897, 2065, 3063, 3873
MfeI CAATTG 4 cut(s) 318, 329, 2166, 3999
MflI RGATCY 8 cut(s) 649, 1178, 1916, 2155, 3045, 3141, 3289, 3454
MhlI GDGCHC 6 cut(s) 1111, 2366, 2386, 3487, 3736, 3917
MlsI TGGCCA 2 cut(s) 594, 742
MluNI TGGCCA 2 cut(s) 594, 742
MlyI GAGTC 2 cut(s) 977, 3881
MmeI TCCRAC 4 cut(s) 1384, 2674, 3267, 3783
Mox20I TGGCCA 2 cut(s) 594, 742
Mph1103I ATGCAT 4 cut(s) 1492, 1771, 2138, 2223
MroNI GCCGGC 1 cut(s) 3501
MroXI GAANNNNTTC 4 cut(s) 309, 1237, 2275, 3339
MscI TGGCCA 2 cut(s) 594, 742
MslI CAYNNNNRTG 1 cut(s) 200
Msp20I TGGCCA 2 cut(s) 594, 742
MspA1I CMGCKG 4 cut(s) 779, 1813, 2645, 3020
MspCI CTTAAG 1 cut(s) 1228
MspI CCGG 2 cut(s) 3409, 3502
MunI CAATTG 4 cut(s) 318, 329, 2166, 3999
Mva1269I GAATGC 3 cut(s) 2034, 2221, 3007
MvnI CGCG 1 cut(s) 1093
NaeI GCCGGC 1 cut(s) 3503
NciI CCSGG 1 cut(s) 3410
NgoMIV GCCGGC 1 cut(s) 3501
NheI GCTAGC 1 cut(s) 3835
NmuCI GTSAC 7 cut(s) 128, 241, 377, 431, 910, 3063, 3873
NsiI ATGCAT 4 cut(s) 1492, 1771, 2138, 2223
NspI RCATGY 3 cut(s) 1148, 1743, 3499
NspV TTCGAA 1 cut(s) 3470
PaeI GCATGC 1 cut(s) 1743
PaeR7I CTCGAG 1 cut(s) 2379
PagI TCATGA 1 cut(s) 3448
PaqCI CACCTGC 1 cut(s) 3719
PciI ACATGT 2 cut(s) 1144, 3495
PciSI GCTCTTC 2 cut(s) 2180, 3645
PctI GAATGC 3 cut(s) 2034, 2221, 3007
PdiI GCCGGC 1 cut(s) 3503
PdmI GAANNNNTTC 4 cut(s) 309, 1237, 2275, 3339
Pfl23II CGTACG 1 cut(s) 1094
PfoI TCCNGGA 1 cut(s) 3007
PleI GAGTC 2 cut(s) 977, 3880
PpsI GAGTC 2 cut(s) 977, 3880
PpuMI RGGWCCY 3 cut(s) 2546, 2636, 3121
PscI ACATGT 2 cut(s) 1144, 3495
Psp124BI GAGCTC 1 cut(s) 2386
Psp5II RGGWCCY 3 cut(s) 2546, 2636, 3121
PspEI GGTNACC 1 cut(s) 3063
PspFI CCCAGC 1 cut(s) 2915
PspLI CGTACG 1 cut(s) 1094
PspPI GGNCC 7 cut(s) 1555, 1601, 2097, 2546, 2636, 2918, 3121
PspPPI RGGWCCY 3 cut(s) 2546, 2636, 3121
PstI CTGCAG 2 cut(s) 550, 823
PstNI CAGNNNCTG 2 cut(s) 1437, 2839
PsuI RGATCY 8 cut(s) 649, 1178, 1916, 2155, 3045, 3141, 3289, 3454
PvuII CAGCTG 3 cut(s) 779, 2645, 3020
RsaI GTAC 5 cut(s) 493, 503, 1096, 3589, 3647
RsaNI GTAC 5 cut(s) 492, 502, 1095, 3588, 3646
RseI CAYNNNNRTG 1 cut(s) 200
SacI GAGCTC 1 cut(s) 2386
SapI GCTCTTC 2 cut(s) 2180, 3645
Sau96I GGNCC 7 cut(s) 1555, 1601, 2097, 2546, 2636, 2918, 3121
SchI GAGTC 2 cut(s) 977, 3881
SduI GDGCHC 6 cut(s) 1111, 2366, 2386, 3487, 3736, 3917
SexAI ACCWGGT 2 cut(s) 874, 3067
SfcI CTRYAG 6 cut(s) 546, 819, 900, 918, 1893, 2766
Sfr274I CTCGAG 1 cut(s) 2379
SfuI TTCGAA 1 cut(s) 3470
SinI GGWCC 5 cut(s) 1601, 2097, 2546, 2636, 3121
SlaI CTCGAG 1 cut(s) 2379
SmiMI CAYNNNNRTG 1 cut(s) 200
SmlI CTYRAG 4 cut(s) 278, 1228, 1964, 2379
SmoI CTYRAG 4 cut(s) 278, 1228, 1964, 2379
SpeI ACTAGT 2 cut(s) 517, 628
SphI GCATGC 1 cut(s) 1743
SspI AATATT 2 cut(s) 976, 2714
SstI GAGCTC 1 cut(s) 2386
TaiI ACGT 2 cut(s) 1963, 3589
TaqI TCGA 7 cut(s) 9, 1071, 2306, 2323, 2380, 3470, 3565
TatI WGTACW 1 cut(s) 501
TauI GCSGC 3 cut(s) 3626, 3661, 3982
TseFI GTSAC 7 cut(s) 128, 241, 377, 431, 910, 3063, 3873
TseI GCWGC 8 cut(s) 818, 839, 1279, 2007, 3020, 3655, 3725, 3976
Tsp45I GTSAC 7 cut(s) 128, 241, 377, 431, 910, 3063, 3873
TspGWI ACGGA 2 cut(s) 3607, 3932
Vha464I CTTAAG 1 cut(s) 1228
VneI GTGCAC 2 cut(s) 1107, 3732
VpaK11BI GGWCC 5 cut(s) 1601, 2097, 2546, 2636, 3121
XagI CCTNNNNNAGG 3 cut(s) 2612, 2925, 3149
XapI RAATTY 8 cut(s) 104, 273, 613, 1065, 1186, 1302, 2275, 3596
XbaI TCTAGA 1 cut(s) 3184
XceI RCATGY 3 cut(s) 1148, 1743, 3499
XcmI CCANNNNNNNNNTGG 3 cut(s) 875, 1595, 2407
XhoI CTCGAG 1 cut(s) 2379
XmiI GTMKAC 2 cut(s) 60, 1395
XmnI GAANNNNTTC 4 cut(s) 309, 1237, 2275, 3339
Zsp2I ATGCAT 4 cut(s) 1492, 1771, 2138, 2223
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.