Rmu_sc0005139.1_g000029

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005139.1
OG0004502
Physical Location & Seq
Reverse (-)
144852 .. 150649
5798 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0005139.1_g000029.1.cds

Sequence Viewer

Length: 3150 bp
atggcgacgggtacgaaggttagggtttcggttttattgtcatatcaggatctcgaagttatagttagatttggtaaaatcgtgattgcaattgatgtgcgaatcgttatatttgactcaaaagcagtcaattcttctccaaatgaggagtttccagggtttgcagatcctgataaagttaaacagtcaggttgtcaaattctcgacagactggaaaataactctcgtacagttaaaggtgcacaaataattggtcaagataatgaagcattagcacatgttttgctaccactaaaaagatgctcgcaaggatcccaaaactctagcagggatcaagatattcttacttcaaaccagtctttaagaatcctttcaaacttagttgcagctggtgccatcacttccagtgggctacttgatgaaatcatacatgaaattcttgtttataccaccttcattgtgggcatcaaatcttctgaactgaatgagtttagagcaaagagtttctcagtaattaaagttttggtagacattgctggacatggcattggaggctcatatttcaggcactgggttgctttagctgatattttttcacaggttgttggtggcagtgaagatgcatctggaagagttttgtttgagtccatagcttgcattactgtcatgttaacaagagttaatcagggtctgaaacaggctgtttctgatcctaatgaagcagtgaaacagattttggatcatgctaaaacatctggtttagtggatcagctttgtctctgcttagctactgctggagcaagtcttatttcaggtccttcaaatatgtcgcgttcagcctgtgaagcatgcagggctatttggttgctggtagatgcatcagagtttttttcgacaaaaggaaatgccatttcctttccgctaaacactttgcgcagttcatcaagggctcaagatcgaggaggttctcagattggaacagaatcagcaagactagtagctgtagttactagagcattagttagatctaaaccagttcaggttgccatccattattgccttcgtcaacgtcttgaggcttcactctgtgctggtattcagctattgttgaggtgctgcttgcagagtggaattgttcctggcattctttgtggcctgcctagttctttacctgtaactactgttgtcagtggtggaggtgatggaaccataatttttgagatattctctttgttgtctgtgtgcatttcatctcaaaataaagatccacaggcaattgagacaaccaacttgaaaagcaaaataaccgatcccactatgttggttctgcattcctgcctcatccttgcatcagttgcacagtgtttaaaggcaactggaagaaattctgcattgtttatgcttactacatcctcgaagaagcagctttctcgactttctgcccttgctcatcacttttcttccggtgagagcacaaatacttcctctcaaactcacagtgcagcagccatgttggcactggcatctattttgtcccttgaatctggagcttctgttgggtcctctgtctttgaggtagcagtgcctttgattcctcaaaccgcgacactttgtgaatgcctcaaactttcatcaaacaatgagattgtatcgggtcctaatggcaccaacggtgttctctcaaactggcatggtcttagggatggatgtgttggcctgctggaggccagactgagatggggaggaccgttggctgttcaacaactttgtgcaagcaatattcctctgcttctaatcaatttgttagccaaaaaccaacaaaatgcttctcctcaagaagttgccagcacaaatgatcaagttgggctttcccctattggagttgtatggacagtatcatctatttgtcagtgcctttctggtggagccttaacctttcgtcagattctgcttagaagtgagcatatcaaactcctctctgacttgatatctgacatgcatctcaaacttgtaaagtcctgggttgggcctggtggaggaatggatggcgtaagagatataacaaacgcagtaattgacctcttggcatttccttttgtggctgttcagaatgctccaggattgccatcagcgactgcctccgtgaacagtgggatccttctgaacatgggttcaccaggtgtgaaagttggcgtggaagacagagacatggtgaaggtgattgaggaagacctcggaaaatatattaaaatccttttggaggttggagtgcctggtatcattctttggtgtctagaacatttggagttaaaagatttgggaagacctgtggcctttctggctaaaatgattggtcaccgaccattggcagttcaacttgtgggtaaaggcttgttggatcctactaggatgagaagattgctcgatcgttcaagcccaaaagaagtcatgctggatgttctgatgattgtttctgatttggcacgcatggacaagggattctatgaatacattaatcgagcttctctgctggagttcttcaagggatttcttacccatgaagatcctagtgtgcgttcgaaggcttgcagtgctctaggcaatatgtgccggcacagctcctacttttacagttccctagcaaagaatcaaatcattggtctccttattgatcgatgctctgacccagacaaacgtacacggaaatttgcttgttttgctattggcaatgccgcttaccataatgatatgttgtatgaagagctgcggagatctatcccccagcttgccaatttgttgctttcatctgaggaagacaagactaaagcaaatgcagcaggtgcactaagcaatcttatccgcaactccaacaaactttgcgaagacattgtctctagaggagccatgcagtctttgctgaagttggtggcagagtgctcagtgttggctctgaacccaagtaggaaagattcagcgcaagagtcacctctcaaaatagctctgttttcattggcaaaaatgtgctcgcatccgccctgcagagatttcctccgttcatctgagttgttccccgtgatcggacggctccgccaatccccggaatcaacaattgccaactacgcctccgccatcatcaacaaagttgcctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000166 GO:0000278 GO:0000280 GO:0000281 GO:0000910 GO:0000911 GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005856 GO:0005874 GO:0005881 GO:0006355 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006935 GO:0006996 GO:0007049 GO:0007112 GO:0007140 GO:0007154 GO:0007163 GO:0007165 GO:0007166 GO:0007224 GO:0007227 GO:0007228 GO:0007275 GO:0007276 GO:0007349 GO:0007584 GO:0008064 GO:0008092 GO:0008134 GO:0008144 GO:0008150 GO:0008152 GO:0008589 GO:0009524 GO:0009553 GO:0009558 GO:0009561 GO:0009605 GO:0009653 GO:0009791 GO:0009889 GO:0009966 GO:0009967 GO:0009987 GO:0009991 GO:0010033 GO:0010243 GO:0010468 GO:0010556 GO:0010639 GO:0010646 GO:0010647 GO:0014070 GO:0015630 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017076 GO:0019219 GO:0019222 GO:0019538 GO:0019894 GO:0019953 GO:0022402 GO:0022412 GO:0022414 GO:0022607 GO:0023051 GO:0023052 GO:0023056 GO:0030010 GO:0030030 GO:0030031 GO:0030554 GO:0030832 GO:0030833 GO:0030837 GO:0031156 GO:0031272 GO:0031323 GO:0031326 GO:0031333 GO:0031344 GO:0031667 GO:0032101 GO:0032104 GO:0032107 GO:0032271 GO:0032272 GO:0032501 GO:0032502 GO:0032504 GO:0032506 GO:0032535 GO:0032553 GO:0032555 GO:0032559 GO:0032956 GO:0032970 GO:0033043 GO:0033206 GO:0033273 GO:0035556 GO:0035639 GO:0036094 GO:0036211 GO:0040011 GO:0042221 GO:0042330 GO:0042493 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043254 GO:0043326 GO:0043327 GO:0043412 GO:0043900 GO:0044085 GO:0044087 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0044703 GO:0044782 GO:0045880 GO:0048229 GO:0048232 GO:0048285 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048609 GO:0048646 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051090 GO:0051091 GO:0051128 GO:0051129 GO:0051171 GO:0051252 GO:0051301 GO:0051321 GO:0051493 GO:0051494 GO:0051593 GO:0051704 GO:0051716 GO:0060176 GO:0060255 GO:0060271 GO:0060491 GO:0061640 GO:0065007 GO:0065008 GO:0065009 GO:0070925 GO:0071704 GO:0071840 GO:0080090 GO:0080134 GO:0080135 GO:0090066 GO:0097159 GO:0097367 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0110053 GO:0120031 GO:0120032 GO:0120035 GO:0120036 GO:0140013 GO:0140096 GO:1901265 GO:1901363 GO:1901564 GO:1901698 GO:1901700 GO:1902410 GO:1902903 GO:1902904 GO:1903046 GO:1903047 GO:1903506 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04341 map04341
Pfam Domains
Protein Families
Arm HEAT HEAT_2 Pkinase

Protein Analysis

1049

Amino Acids

112.68

Weight (kDa)

8.56

Isoelectric Point (pI)

46.25

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2828
Acc16I TGCGCA 1 cut(s) 935
Acc36I ACCTGC 1 cut(s) 2828
AccB1I GGYRCC 2 cut(s) 392, 1664
AccI GTMKAC 1 cut(s) 528
AccII CGCG 2 cut(s) 832, 1604
AciI CCGC 8 cut(s) 920, 1602, 2733, 2767, 2860, 3032, 3088, 3126
AcsI RAATTY 4 cut(s) 198, 435, 1384, 2705
AcuI CTGAAG 1 cut(s) 2939
AdeI CACNNNGTG 3 cut(s) 1088, 1613, 2180
AfaI GTAC 3 cut(s) 13, 229, 2698
AfiI CCNNNNNNNGG 7 cut(s) 1723, 2025, 2036, 2260, 2365, 3077, 3097
AflIII ACRYGT 1 cut(s) 277
AgsI TTSAA 9 cut(s) 351, 375, 822, 1294, 1541, 1760, 2375, 2433, 2542
AhlI ACTAGT 1 cut(s) 994
AjnI CCWGG 7 cut(s) 154, 1138, 2018, 2029, 2116, 2176, 2272
AjuI GAANNNNNNNTTGG 2 cut(s) 719, 751
Alw21I GWGCWC 6 cut(s) 244, 1475, 2596, 2845, 2939, 3026
Alw26I GTCTC 5 cut(s) 782, 1274, 2199, 2666, 2896
Alw44I GTGCAC 2 cut(s) 240, 2841
AlwNI CAGNNNCTG 5 cut(s) 170, 570, 691, 1948, 2135
AoxI GGCC 5 cut(s) 1153, 1714, 1725, 2027, 2331
ApaLI GTGCAC 2 cut(s) 240, 2841
ApeKI GCWGC 7 cut(s) 386, 1116, 1423, 1502, 1505, 2764, 2834
ApoI RAATTY 4 cut(s) 198, 435, 1384, 2705
ArsI GACNNNNNNTTYG 6 cut(s) 238, 270, 2644, 2676, 2874, 2906
AseI ATTAAT 1 cut(s) 2514
Asp700I GAANNNNTTC 2 cut(s) 370, 1384
AspLEI GCGC 2 cut(s) 936, 2977
AspS9I GGNCC 5 cut(s) 815, 1560, 1655, 1745, 2027
AsuC2I CCSGG 1 cut(s) 3098
AsuHPI GGTGA 7 cut(s) 1211, 1478, 2166, 2224, 2230, 2348, 2976
AsuII TTCGAA 1 cut(s) 2579
AvaII GGWCC 4 cut(s) 815, 1560, 1655, 1745
BaeGI GKGCMC 2 cut(s) 244, 2845
BaeI ACNNNNGTAYC 1 cut(s) 36
BamHI GGATCC 3 cut(s) 311, 2154, 2398
BanI GGYRCC 2 cut(s) 392, 1664
BanII GRGCYC 1 cut(s) 952
BbsI GAAGAC 5 cut(s) 2205, 2235, 2329, 2820, 2889
Bbv12I GWGCWC 6 cut(s) 244, 1475, 2596, 2845, 2939, 3026
BbvI GCAGC 7 cut(s) 398, 1103, 1435, 1514, 1517, 2751, 2846
BccI CCATC 8 cut(s) 404, 1055, 1196, 1697, 1731, 2039, 2134, 3137
BceAI ACGGC 1 cut(s) 3098
BcgI CGANNNNNNTGC 2 cut(s) 1412, 1446
BciT130I CCWGG 7 cut(s) 156, 1140, 2020, 2031, 2118, 2178, 2274
BclI TGATCA 1 cut(s) 1855
BcnI CCSGG 1 cut(s) 3098
BcoDI GTCTC 5 cut(s) 782, 1274, 2199, 2666, 2896
BcuI ACTAGT 1 cut(s) 994
BfmI CTRYAG 2 cut(s) 1002, 3037
BfuAI ACCTGC 1 cut(s) 2828
BglI GCCNNNNNGGC 2 cut(s) 1514, 2339
BglII AGATCT 2 cut(s) 1025, 2771
BisI GCNGC 8 cut(s) 387, 1117, 1424, 1503, 1506, 2733, 2765, 2835
BlpI GCTNAGC 1 cut(s) 784
BlsI GCNGC 8 cut(s) 388, 1118, 1425, 1504, 1507, 2734, 2766, 2836
Bme1390I CCNGG 8 cut(s) 156, 1140, 2020, 2031, 2118, 2178, 2274, 3098
Bme18I GGWCC 4 cut(s) 815, 1560, 1655, 1745
BmgT120I GGNCC 5 cut(s) 815, 1560, 1655, 1745, 2027
BmrFI CCNGG 8 cut(s) 156, 1140, 2020, 2031, 2118, 2178, 2274, 3098
BmrI ACTGGG 1 cut(s) 580
BmuI ACTGGG 1 cut(s) 580
BpiI GAAGAC 5 cut(s) 2205, 2235, 2329, 2820, 2889
BplI GAGNNNNNCTC 4 cut(s) 1211, 1243, 3033, 3065
BpmI CTGGAG 5 cut(s) 816, 1566, 1742, 2100, 2552
Bpu1102I GCTNAGC 1 cut(s) 784
Bpu14I TTCGAA 1 cut(s) 2579
BpuEI CTTGAG 3 cut(s) 936, 1094, 1818
BpuMI CCSGG 1 cut(s) 3098
Bsa29I ATCGAT 1 cut(s) 2674
BsaBI GATNNNNATC 1 cut(s) 2125
BsaI GGTCTC 1 cut(s) 2666
BsaJI CCNNGG 4 cut(s) 155, 2019, 2233, 3096
BsaWI WCCGGW 1 cut(s) 1463
BsaXI ACNNNNNCTCC 4 cut(s) 1539, 1569, 3107, 3137
Bsc4I CCNNNNNNNGG 7 cut(s) 1723, 2025, 2036, 2260, 2365, 3077, 3097
Bse118I RCCGGY 1 cut(s) 2610
Bse1I ACTGG 8 cut(s) 216, 355, 405, 575, 1034, 1381, 1524, 1691
Bse3DI GCAATG 2 cut(s) 531, 2734
Bse8I GATNNNNATC 1 cut(s) 2125
BseBI CCWGG 7 cut(s) 156, 1140, 2020, 2031, 2118, 2178, 2274
BseCI ATCGAT 1 cut(s) 2674
BseDI CCNNGG 4 cut(s) 155, 2019, 2233, 3096
BseGI GGATG 9 cut(s) 1047, 1341, 1409, 1708, 1712, 2050, 2415, 2461, 3028
BseJI GATNNNNATC 1 cut(s) 2125
BseLI CCNNNNNNNGG 7 cut(s) 1723, 2025, 2036, 2260, 2365, 3077, 3097
BseMI GCAATG 2 cut(s) 531, 2734
BseMII CTCAG 6 cut(s) 522, 983, 1724, 2799, 2952, 3051
BseNI ACTGG 8 cut(s) 216, 355, 405, 575, 1034, 1381, 1524, 1691
BseRI GAGGAG 5 cut(s) 161, 975, 1821, 1964, 2913
BseSI GKGCMC 2 cut(s) 244, 2845
BseXI GCAGC 7 cut(s) 398, 1103, 1435, 1514, 1517, 2751, 2846
BseYI CCCAGC 1 cut(s) 2781
BsgI GTGCAG 1 cut(s) 1521
Bsh1236I CGCG 2 cut(s) 832, 1604
Bsh1285I CGRYCG 1 cut(s) 2428
BshFI GGCC 5 cut(s) 1155, 1716, 1727, 2029, 2333
BshNI GGYRCC 2 cut(s) 392, 1664
BshVI ATCGAT 1 cut(s) 2674
BsiEI CGRYCG 1 cut(s) 2428
BsiHKAI GWGCWC 6 cut(s) 244, 1475, 2596, 2845, 2939, 3026
BsiSI CCGG 3 cut(s) 1464, 2611, 3098
BslFI GGGAC 1 cut(s) 1519
BslI CCNNNNNNNGG 7 cut(s) 1723, 2025, 2036, 2260, 2365, 3077, 3097
BsmAI GTCTC 5 cut(s) 782, 1274, 2199, 2666, 2896
BsmFI GGGAC 1 cut(s) 1519
BsmI GAATGC 4 cut(s) 1143, 1330, 1622, 2116
BsnI GGCC 5 cut(s) 1155, 1716, 1727, 2029, 2333
Bso31I GGTCTC 1 cut(s) 2666
Bsp119I TTCGAA 1 cut(s) 2579
Bsp1286I GDGCHC 7 cut(s) 244, 952, 1475, 2596, 2845, 2939, 3026
Bsp1720I GCTNAGC 1 cut(s) 784
BspACI CCGC 8 cut(s) 920, 1602, 2733, 2767, 2860, 3032, 3088, 3126
BspANI GGCC 5 cut(s) 1155, 1716, 1727, 2029, 2333
BspCNI CTCAG 6 cut(s) 521, 982, 1725, 2800, 2951, 3052
BspDI ATCGAT 1 cut(s) 2674
BspFNI CGCG 2 cut(s) 832, 1604
BspMAI CTGCAG 1 cut(s) 3041
BspMI ACCTGC 1 cut(s) 2828
BspQI GCTCTTC 1 cut(s) 2754
BspT104I TTCGAA 1 cut(s) 2579
BspT107I GGYRCC 2 cut(s) 392, 1664
BspTNI GGTCTC 1 cut(s) 2666
BsrDI GCAATG 2 cut(s) 531, 2734
BsrFI RCCGGY 1 cut(s) 2610
BsrI ACTGG 8 cut(s) 216, 355, 405, 575, 1034, 1381, 1524, 1691
BssAI RCCGGY 1 cut(s) 2610
BssECI CCNNGG 4 cut(s) 155, 2019, 2233, 3096
Bst2UI CCWGG 7 cut(s) 156, 1140, 2020, 2031, 2118, 2178, 2274
Bst6I CTCTTC 2 cut(s) 625, 2754
BstAPI GCANNNNNTGC 5 cut(s) 392, 1355, 2607, 2840, 2914
BstBI TTCGAA 1 cut(s) 2579
BstEII GGTNACC 1 cut(s) 2354
BstENI CCTNNNNNAGG 3 cut(s) 1721, 2034, 2258
BstF5I GGATG 9 cut(s) 1047, 1341, 1409, 1708, 1712, 2050, 2415, 2461, 3028
BstFNI CGCG 2 cut(s) 832, 1604
BstHHI GCGC 2 cut(s) 936, 2977
BstMAI GTCTC 5 cut(s) 782, 1274, 2199, 2666, 2896
BstMCI CGRYCG 1 cut(s) 2428
BstNI CCWGG 7 cut(s) 156, 1140, 2020, 2031, 2118, 2178, 2274
BstNSI RCATGY 3 cut(s) 281, 852, 1999
BstPI GGTNACC 1 cut(s) 2354
BstSCI CCNGG 8 cut(s) 154, 1138, 2018, 2029, 2116, 2176, 2272, 3096
BstSFI CTRYAG 2 cut(s) 1002, 3037
BstSLI GKGCMC 2 cut(s) 244, 2845
BstUI CGCG 2 cut(s) 832, 1604
BstV1I GCAGC 7 cut(s) 398, 1103, 1435, 1514, 1517, 2751, 2846
BstV2I GAAGAC 5 cut(s) 2205, 2235, 2329, 2820, 2889
BstX2I RGATCY 9 cut(s) 49, 166, 311, 1025, 1264, 2154, 2398, 2563, 2771
BstXI CCANNNNNNTGG 1 cut(s) 1321
BstYI RGATCY 9 cut(s) 49, 166, 311, 1025, 1264, 2154, 2398, 2563, 2771
Bsu15I ATCGAT 1 cut(s) 2674
BsuRI GGCC 5 cut(s) 1155, 1716, 1727, 2029, 2333
BsuTUI ATCGAT 1 cut(s) 2674
BtsCI GGATG 9 cut(s) 1047, 1341, 1409, 1708, 1712, 2050, 2415, 2461, 3028
BtsI GCAGTG 4 cut(s) 619, 729, 1587, 2596
BveI ACCTGC 1 cut(s) 2828
CaiI CAGNNNCTG 5 cut(s) 170, 570, 691, 1948, 2135
CfoI GCGC 2 cut(s) 936, 2977
Cfr10I RCCGGY 1 cut(s) 2610
Cfr13I GGNCC 5 cut(s) 815, 1560, 1655, 1745, 2027
ClaI ATCGAT 1 cut(s) 2674
CsiI ACCWGGT 1 cut(s) 2176
Csp6I GTAC 3 cut(s) 12, 228, 2697
CspCI CAANNNNNGTGG 2 cut(s) 439, 474
CviQI GTAC 3 cut(s) 12, 228, 2697
DraI TTTAAA 1 cut(s) 1368
DraIII CACNNNGTG 3 cut(s) 1088, 1613, 2180
Eam1104I CTCTTC 2 cut(s) 625, 2754
EarI CTCTTC 2 cut(s) 625, 2754
EciI GGCGGA 3 cut(s) 3021, 3077, 3115
Eco24I GRGCYC 1 cut(s) 952
Eco31I GGTCTC 1 cut(s) 2666
Eco32I GATATC 1 cut(s) 1989
Eco47I GGWCC 4 cut(s) 815, 1560, 1655, 1745
Eco57I CTGAAG 1 cut(s) 2939
Eco91I GGTNACC 1 cut(s) 2354
EcoNI CCTNNNNNAGG 3 cut(s) 1721, 2034, 2258
EcoO109I RGGNCCY 3 cut(s) 815, 1560, 1655
EcoO65I GGTNACC 1 cut(s) 2354
EcoRII CCWGG 7 cut(s) 154, 1138, 2018, 2029, 2116, 2176, 2272
EcoRV GATATC 1 cut(s) 1989
EcoT22I ATGCAT 3 cut(s) 625, 880, 2001
EcoT38I GRGCYC 1 cut(s) 952
FalI AAGNNNNNCTT 4 cut(s) 327, 359, 1851, 1883
FaqI GGGAC 1 cut(s) 1519
FbaI TGATCA 1 cut(s) 1855
FblI GTMKAC 1 cut(s) 528
Fnu4HI GCNGC 8 cut(s) 387, 1117, 1424, 1503, 1506, 2733, 2765, 2835
FokI GGATG 9 cut(s) 1034, 1328, 1396, 1715, 1719, 2057, 2422, 2468, 3015
FriOI GRGCYC 1 cut(s) 952
Fsp4HI GCNGC 8 cut(s) 387, 1117, 1424, 1503, 1506, 2733, 2765, 2835
FspI TGCGCA 1 cut(s) 935
GlaI GCGC 2 cut(s) 935, 2976
GluI GCNGC 8 cut(s) 387, 1117, 1424, 1503, 1506, 2733, 2765, 2835
GsaI CCCAGC 1 cut(s) 2785
GsuI CTGGAG 5 cut(s) 816, 1566, 1742, 2100, 2552
HaeIII GGCC 5 cut(s) 1155, 1716, 1727, 2029, 2333
HapII CCGG 3 cut(s) 1464, 2611, 3098
HhaI GCGC 2 cut(s) 936, 2977
Hin6I GCGC 2 cut(s) 934, 2975
HinP1I GCGC 2 cut(s) 934, 2975
HincII GTYRAC 2 cut(s) 672, 1067
HindII GTYRAC 2 cut(s) 672, 1067
HpaI GTTAAC 1 cut(s) 672
HpaII CCGG 3 cut(s) 1464, 2611, 3098
HphI GGTGA 7 cut(s) 1211, 1478, 2166, 2224, 2230, 2348, 2976
Hpy166II GTNNAC 8 cut(s) 242, 529, 672, 1067, 2146, 2174, 2699, 2843
Hpy8I GTNNAC 8 cut(s) 242, 529, 672, 1067, 2146, 2174, 2699, 2843
Hpy99I CGWCG 1 cut(s) 10
HpyAV CCTTC 7 cut(s) 10, 463, 828, 1070, 2168, 2209, 2575
HpyCH4IV ACGT 2 cut(s) 1069, 2695
HpySE526I ACGT 2 cut(s) 1069, 2695
HspAI GCGC 2 cut(s) 934, 2975
KroI GCCGGC 1 cut(s) 2610
KroNI GCCGGC 1 cut(s) 2612
Ksp22I TGATCA 1 cut(s) 1855
KspAI GTTAAC 1 cut(s) 672
LguI GCTCTTC 1 cut(s) 2754
LmnI GCTCC 7 cut(s) 797, 1547, 1925, 2119, 2624, 2900, 3090
Lsp1109I GCAGC 7 cut(s) 398, 1103, 1435, 1514, 1517, 2751, 2846
MabI ACCWGGT 1 cut(s) 2176
MaeII ACGT 2 cut(s) 1069, 2695
MaeIII GTNAC 4 cut(s) 1006, 1174, 2354, 2982
MfeI CAATTG 3 cut(s) 90, 1275, 3108
MflI RGATCY 9 cut(s) 49, 166, 311, 1025, 1264, 2154, 2398, 2563, 2771
MhlI GDGCHC 7 cut(s) 244, 952, 1475, 2596, 2845, 2939, 3026
MlyI GAGTC 3 cut(s) 110, 653, 2990
MmeI TCCRAC 3 cut(s) 2245, 2376, 2892
Mph1103I ATGCAT 3 cut(s) 625, 880, 2001
MroNI GCCGGC 1 cut(s) 2610
MroXI GAANNNNTTC 2 cut(s) 370, 1384
MspA1I CMGCKG 1 cut(s) 389
MspI CCGG 3 cut(s) 1464, 2611, 3098
MspR9I CCNGG 8 cut(s) 156, 1140, 2020, 2031, 2118, 2178, 2274, 3098
MunI CAATTG 3 cut(s) 90, 1275, 3108
Mva1269I GAATGC 4 cut(s) 1143, 1330, 1622, 2116
MvaI CCWGG 7 cut(s) 156, 1140, 2020, 2031, 2118, 2178, 2274
MvnI CGCG 2 cut(s) 832, 1604
NaeI GCCGGC 1 cut(s) 2612
NciI CCSGG 1 cut(s) 3098
NgoMIV GCCGGC 1 cut(s) 2610
NmuCI GTSAC 2 cut(s) 2354, 2982
NsbI TGCGCA 1 cut(s) 935
NsiI ATGCAT 3 cut(s) 625, 880, 2001
NspI RCATGY 3 cut(s) 281, 852, 1999
NspV TTCGAA 1 cut(s) 2579
PaeI GCATGC 1 cut(s) 852
PaqCI CACCTGC 1 cut(s) 2828
PciI ACATGT 1 cut(s) 277
PciSI GCTCTTC 1 cut(s) 2754
PctI GAATGC 4 cut(s) 1143, 1330, 1622, 2116
PdiI GCCGGC 1 cut(s) 2612
PdmI GAANNNNTTC 2 cut(s) 370, 1384
PflFI GACNNNGTC 1 cut(s) 2888
PfoI TCCNGGA 1 cut(s) 2116
PkrI GCNGC 8 cut(s) 388, 1118, 1425, 1504, 1507, 2734, 2766, 2836
Ple19I CGATCG 1 cut(s) 2428
PleI GAGTC 3 cut(s) 110, 652, 2989
PpsI GAGTC 3 cut(s) 110, 652, 2989
PpuMI RGGWCCY 3 cut(s) 815, 1560, 1655
PscI ACATGT 1 cut(s) 277
PshBI ATTAAT 1 cut(s) 2514
Psp5II RGGWCCY 3 cut(s) 815, 1560, 1655
Psp6I CCWGG 7 cut(s) 154, 1138, 2018, 2029, 2116, 2176, 2272
PspEI GGTNACC 1 cut(s) 2354
PspFI CCCAGC 1 cut(s) 2781
PspGI CCWGG 7 cut(s) 154, 1138, 2018, 2029, 2116, 2176, 2272
PspPI GGNCC 5 cut(s) 815, 1560, 1655, 1745, 2027
PspPPI RGGWCCY 3 cut(s) 815, 1560, 1655
PstI CTGCAG 1 cut(s) 3041
PstNI CAGNNNCTG 5 cut(s) 170, 570, 691, 1948, 2135
PsuI RGATCY 9 cut(s) 49, 166, 311, 1025, 1264, 2154, 2398, 2563, 2771
PsyI GACNNNGTC 1 cut(s) 2888
PvuI CGATCG 1 cut(s) 2428
PvuII CAGCTG 1 cut(s) 389
RsaI GTAC 3 cut(s) 13, 229, 2698
RsaNI GTAC 3 cut(s) 12, 228, 2697
SapI GCTCTTC 1 cut(s) 2754
SatI GCNGC 8 cut(s) 387, 1117, 1424, 1503, 1506, 2733, 2765, 2835
Sau96I GGNCC 5 cut(s) 815, 1560, 1655, 1745, 2027
SchI GAGTC 3 cut(s) 110, 653, 2990
ScrFI CCNGG 8 cut(s) 156, 1140, 2020, 2031, 2118, 2178, 2274, 3098
SduI GDGCHC 7 cut(s) 244, 952, 1475, 2596, 2845, 2939, 3026
SexAI ACCWGGT 1 cut(s) 2176
SfcI CTRYAG 2 cut(s) 1002, 3037
SfuI TTCGAA 1 cut(s) 2579
SinI GGWCC 4 cut(s) 815, 1560, 1655, 1745
SmlI CTYRAG 3 cut(s) 951, 1073, 1833
SmoI CTYRAG 3 cut(s) 951, 1073, 1833
SpeI ACTAGT 1 cut(s) 994
SphI GCATGC 1 cut(s) 852
SsiI CCGC 8 cut(s) 920, 1602, 2733, 2767, 2860, 3032, 3088, 3126
SspI AATATT 1 cut(s) 1780
StyD4I CCNGG 8 cut(s) 154, 1138, 2018, 2029, 2116, 2176, 2272, 3096
TaiI ACGT 2 cut(s) 1072, 2698
TauI GCSGC 1 cut(s) 2735
TseFI GTSAC 2 cut(s) 2354, 2982
TseI GCWGC 7 cut(s) 386, 1116, 1423, 1502, 1505, 2764, 2834
Tsp45I GTSAC 2 cut(s) 2354, 2982
TspGWI ACGGA 3 cut(s) 2131, 2716, 3041
Tth111I GACNNNGTC 1 cut(s) 2888
VneI GTGCAC 2 cut(s) 240, 2841
VpaK11BI GGWCC 4 cut(s) 815, 1560, 1655, 1745
VspI ATTAAT 1 cut(s) 2514
XagI CCTNNNNNAGG 3 cut(s) 1721, 2034, 2258
XapI RAATTY 4 cut(s) 198, 435, 1384, 2705
XbaI TCTAGA 2 cut(s) 2293, 2894
XceI RCATGY 3 cut(s) 281, 852, 1999
XcmI CCANNNNNNNNNTGG 2 cut(s) 457, 1516
XmiI GTMKAC 1 cut(s) 528
XmnI GAANNNNTTC 2 cut(s) 370, 1384
Zsp2I ATGCAT 3 cut(s) 625, 880, 2001
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.