Rh1AG468800

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0004502
Physical Location & Seq
Forward (+)
68220987 .. 68230031
9045 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG468800.1

Sequence Viewer

Length: 4095 bp
ATGGGTGTCAAGAATTATCATGTAATTGAGCTTGTAGGCGAAGGTTCCTTTGGGAAGGTATACAAGGGAAGACGCAAGTTCACTGGGCAGACCGTTGCAATGAAATTTATTATGAAACATGGGAAAAGCGACAAGGATATCCACAATTTAAGACAAGAAATAGAGATTCTAAGGAAGCTAAAACATGAAAATATCATTGAAATGCTTGATTCCTTTGAAAGCCCACAAGAATTCTGTGTTGTCACAGAGTTTGCACAAGGTGAACTATTTGAAATTCTTGAAGATGATAAGCACCTTCCAGAAGAACAAGTTCAAGCAATTGCTAAGCAATTGGTGAGAGCATTGCATTATCTCCATTCAAACCGCATCATCCATCGTGACATGAAACCACAAAACATTCTCATTGGTGCTGGTTCTATTGTTAAGCTCTGTGACTTTGGGTTTGCACGTGCAATGTCCACAAATACTGTTGTTTTGCGATCCATAAAAGGTACTCCCCTGTACATGGCTCCTGAACTAGTACGGGAACAACCTTACAACCACACTGCAGATTTATGGTCTCTCGGAGTTATATTATATGAATTATTTGTTGGCCAGCCTCCATTTTATACAAATTCAGTATATGCACTCATTCGGCATATTGTGAAGGATCCAGTTAAATACCCTGACAACATGAGTTCAAATTTTAAAAACTTTCTGAAGGGATTGCTCAATAAGATGCCTCAAAATCGTTTGACTTGGCCAGCTCTTTTGGAACACCCATTTGTTAAGGAGACAACTGAAGAACTGGAGGCTAGGGAAATGCGTTCTGCTACTGCTGCAGAAAGGGGGTGCGTTGCAGCATGGAGGGGTGAAGGAAACAAAATCCAAACATCAGGTGGCTTGGCTGTTTCTAGTCCTGGTAACACGTTATTCTGTTCTTTAAGTGTTTCTTTTTTTTTCTTTTTCTTTCAGTTGTCATTAACTTCTTACCATGTAATTGGTAAAGTCAACTCTCCAGCTTCTTCAGAGAACAACAGTGGAATAAGTTTCCAAAATGATGCTCAAACAGATATTCCAGACTCTAAAGCAGTCAATTCTTCTCCAAATGAGGAGTTTCCAGGGTTTGCAAATCCTGATAAAGTTAAACAGTCAGGTTGTCAAATTCTCGACAGACTGGAAAATAACTCTCGTACAGTTAAAGGTGCACAAATAATTGGTCAAGATAATGAAGCATTAGCACATGTTTTGCTACCACTAAAAAGATGCTCGCAAGGATCCCAAAACTCTAGCAGGGATCAAGATATTCTTACTTCAAACCAGTCTTTAAGAATCCTTTCAAACTTAGTTGCAGCTGGTGCCATCACTTCCAGTGACCTACTTGATGAAATCATACATGAAATTCTTGTTTATACCACCTTCATTGTGGGCATCAAATCTTCTGAACTGAATGAGTTTAGAGCAAAGAGTTTCTCAGTAATTAAAGTTTTGGTAGACATTGCTGGACATGGCATTGGAGGCTCATATTTCAGGCACTGGGTTGCTTTAGCTGATATTTTTTCACAGGTTGTTGGTGGCAGTGAAGATGCATCTGGAAGAGTTTTGTTTGAGTCCATAGCTTGCATTACTGTCATGTTAACAAGAGTTAATCAGGGTCTGAAACAGGCTGTTTCTGATCCTAATGAAGCAGTGAAACAGATTTTGGATCATGCTAAAACATCTGGTTTAGTGGATCAGCTTTGTCTCTGCTTAGCTACTGCTGGAGCAAGTCTTATTTCGGGTCCTTCAAATATGTCGCGTTCAGCCTGTGAAGCATGCATGGCTATTTGGTTGCTGGTAGATACATCAGAGTTTTTTTCGACAAAAGGAAATGCCATTTCCTTTCCGCTAAACACTTTGCGCAGTCCATCAAGGGCTCAAGATCGAGGAGGTTCTTTGATTGGAACAGAATCAGCAAGACTAGTAGCTGTAGTTACTAGAGCATTAGTTAGATCTAAACCAGTTCAGGTTGCCATCCATTATTGCCTTCGTCAACGTCTTGAGGCTTCACTCTGTGCTGGTATTCAGCTATTGTTGAGGTGCTGCTTGCAGAGTGGAATTGTTCCTGGCATTCTTTGTGGCCTGCCTAGTTCTTTACCTGTAACTACTGTTGTCAGTGGTGGAGGTGATGGAACCATAATTTTTGAGATATTCTCTTTGTTGTCTGTGTGCATTTCATCTCAAAATAAAGATCCACAAGCAATTGAGACAACCAACTTGAAGAGCAAAATAACCGATCCCACTATGTTGGTTCTGCATTCCTGCCTCATCCTTGCATCAGTTGCACAATGTTTAAAGGCAACTGGAAGAAATTCTGCATTGTTTATGCTTACTACATCCTCGAAGAAGCAGCTTTCTCGACTTTCTGCCCTTGCTCATCACTTTTCTTCCGGTGAGAGCACAAATACTTCCTCTCAAACTCACAGTGCAGCAGCCATGTTGGCACTGGCATCTATTTTGTCCCTTGAATCTGGAGCTTCTGTTGGGTCCTCTGTCTTTGAGGTAGCAGTGCCTTTGATTCCTCAAACTGCGACACTTTGTGAATGCCTCAAACTTTCATCAAACAATGAGATTGTATCGGGTCCTAATGGCACCAACGGTGTTCTCTCAAACTGGCATGGTCTTAGGGATGGATGTGTTGGCTTGCTGGAGGCCAGACTGAGATGGGGAGGACCCTTAGCTGTTCAACAACTTTGTGCAAGCAATATCCCTCTGCTTCTAATCAATTTGTTAGCCAAAAACCAACAAAATGCTTCTCCTCAAGAAGTTGCCAGCACAAATGATCAAGTTGGGCTTTCCCCTATTGGAGTTGTATGGACAGTATCATCTATTTGTCAGTGCCTTTCTGGTGGAGCATTAACCTTTCGTCAGATTCTGCTTAGAAGTGAGCATATCAAACTCCTCTCTGACTTGATATCTGACATGCATCTCAAACTTGTAAAGTCCTGGGTTGGGCCTGGTGGAGGAATGGATGGCGTAAGAGATATAACAAATGCAGTAATTGACCTCTTGGCATTTCCTTTTGTGGCTGTTCAGAATGCTCCAGGATTGCCATCAGCGACTGCCTCAGTGAACAGTGGGATCCTTCTGAACATGGGTTCACCAGGTGTGAAAGTTGGCGTTGAAGACAGAGACATGGTGAAGGTGATTGAGGAAGACCTGGGAAAATATATTAAAATCCTTTTGGAGGTTGGAGTGCCTGGTATCATTCTTTGGTGTCTAGAACATTTGGAGTTAAAAGATTTGGGAAGACCTGTGGCCTTTCTGGCTAAAATGATTGGTCACCGACCATTGGCAGTTCAACTTGTGGGTAAGGGCTTGTTGGATCCTACTAGGATGAGAAGATTGCTCGATCGTTCAAGCCCAAAAGAAGTCATGCTGGATGTTCTGATGATTGTTTCTGATTTGGCACGCATGGACAAGGGATTCTATGAATACATTAATCGAGCTTCTCTGCTGGAGTTCTTCAAGGGATTTCTTACCCATGAAGATCCTAGTGTGCGTTCGAAGGCTTGCAGTGCTCTAGGCAATATGTGCCGGCACAGCTCCTACTTTTACAGTTCCCTAGCAAAGAATCAAATCATTGGTCTCCTTATTGATCGATGCTCTGACCCAGACAAACGTACACGGAAATTTGCTTGTTTTGCTATTGGCAATGCCGCTTACCATAATGATATGTTGTATGAAGAGCTGCGGAGATCTATCCCCCAGCTTGCCAATTTGTTGCTTTCATCTGAGGAAGACAAGACTAAAGCAAATGCAGCAGGTGCACTAAGCAATCTTATCCGCAACTCCAACAAACTTTGCGAAGACATTGTCTCTAGAGGAGCCATGCAGTCTTTGCTGAAGTTGGTGGCAGAGTGCTCAGTGTTGGCTCTGAACCCAAGTAGGAAAGATTCAGCGCAAGAGTCACCTCTCAAAATAGCTCTGTTTTCATTGGCAAAAATGTGCTCGCATCCGCCCTGCAGAGATTTCCTCCGTTCATCTGAGTTGTTCCCCGTGATCGGACGGCTCCGCCAATCCCCGGAATCAACAATTGCCAACTACGCCTCCGCCATCATCAACAAAGTTGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000166 GO:0000278 GO:0000280 GO:0000281 GO:0000910 GO:0000911 GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005856 GO:0005874 GO:0005881 GO:0006355 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006935 GO:0006996 GO:0007049 GO:0007112 GO:0007140 GO:0007154 GO:0007163 GO:0007165 GO:0007166 GO:0007224 GO:0007227 GO:0007228 GO:0007275 GO:0007276 GO:0007349 GO:0007584 GO:0008064 GO:0008092 GO:0008134 GO:0008144 GO:0008150 GO:0008152 GO:0008589 GO:0009524 GO:0009553 GO:0009558 GO:0009561 GO:0009605 GO:0009653 GO:0009791 GO:0009889 GO:0009966 GO:0009967 GO:0009987 GO:0009991 GO:0010033 GO:0010243 GO:0010468 GO:0010556 GO:0010639 GO:0010646 GO:0010647 GO:0014070 GO:0015630 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017076 GO:0019219 GO:0019222 GO:0019538 GO:0019894 GO:0019953 GO:0022402 GO:0022412 GO:0022414 GO:0022607 GO:0023051 GO:0023052 GO:0023056 GO:0030010 GO:0030030 GO:0030031 GO:0030554 GO:0030832 GO:0030833 GO:0030837 GO:0031156 GO:0031272 GO:0031323 GO:0031326 GO:0031333 GO:0031344 GO:0031667 GO:0032101 GO:0032104 GO:0032107 GO:0032271 GO:0032272 GO:0032501 GO:0032502 GO:0032504 GO:0032506 GO:0032535 GO:0032553 GO:0032555 GO:0032559 GO:0032956 GO:0032970 GO:0033043 GO:0033206 GO:0033273 GO:0035556 GO:0035639 GO:0036094 GO:0036211 GO:0040011 GO:0042221 GO:0042330 GO:0042493 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043254 GO:0043326 GO:0043327 GO:0043412 GO:0043900 GO:0044085 GO:0044087 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0044703 GO:0044782 GO:0045880 GO:0048229 GO:0048232 GO:0048285 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048609 GO:0048646 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051090 GO:0051091 GO:0051128 GO:0051129 GO:0051171 GO:0051252 GO:0051301 GO:0051321 GO:0051493 GO:0051494 GO:0051593 GO:0051704 GO:0051716 GO:0060176 GO:0060255 GO:0060271 GO:0060491 GO:0061640 GO:0065007 GO:0065008 GO:0065009 GO:0070925 GO:0071704 GO:0071840 GO:0080090 GO:0080134 GO:0080135 GO:0090066 GO:0097159 GO:0097367 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0110053 GO:0120031 GO:0120032 GO:0120035 GO:0120036 GO:0140013 GO:0140096 GO:1901265 GO:1901363 GO:1901564 GO:1901698 GO:1901700 GO:1902410 GO:1902903 GO:1902904 GO:1903046 GO:1903047 GO:1903506 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04341 map04341
Pfam Domains
Protein Families
Arm HEAT HEAT_2 Pkinase

Protein Analysis

1364

Amino Acids

148.41

Weight (kDa)

8.35

Isoelectric Point (pI)

48.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 6 - 256 1.2e-78 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 8 - 251 3.5e-47 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 3773
Acc16I TGCGCA 1 cut(s) 1880
Acc36I ACCTGC 1 cut(s) 3773
AccB1I GGYRCC 2 cut(s) 1337, 2609
AccI GTMKAC 2 cut(s) 60, 1473
AccII CGCG 1 cut(s) 1777
AciI CCGC 8 cut(s) 364, 1865, 3678, 3712, 3805, 3977, 4033, 4071
AcoI YGGCCR 2 cut(s) 592, 740
AcsI RAATTY 9 cut(s) 104, 230, 273, 613, 682, 1143, 1380, 2329, 3650
AcuI CTGAAG 4 cut(s) 719, 801, 990, 3884
AcvI CACGTG 1 cut(s) 449
AdeI CACNNNGTG 4 cut(s) 260, 2033, 2558, 3125
AfaI GTAC 5 cut(s) 493, 503, 522, 1174, 3643
AfiI CCNNNNNNNGG 7 cut(s) 505, 2970, 2981, 3205, 3310, 4022, 4042
AflIII ACRYGT 2 cut(s) 906, 1222
AhlI ACTAGT 2 cut(s) 517, 1939
AjnI CCWGG 9 cut(s) 898, 1099, 2083, 2963, 2974, 3061, 3121, 3177, 3217
AjuI GAANNNNNNNTTGG 6 cut(s) 33, 65, 573, 605, 1664, 1696
Alw21I GWGCWC 6 cut(s) 1189, 2420, 3541, 3790, 3884, 3971
Alw26I GTCTC 7 cut(s) 564, 767, 1727, 2219, 3144, 3611, 3841
Alw44I GTGCAC 2 cut(s) 1185, 3786
AlwNI CAGNNNCTG 4 cut(s) 1515, 1636, 2893, 3080
AoxI GGCC 6 cut(s) 592, 740, 2098, 2670, 2972, 3276
ApaLI GTGCAC 2 cut(s) 1185, 3786
ApeKI GCWGC 9 cut(s) 818, 839, 1331, 2061, 2368, 2447, 2450, 3709, 3779
ApoI RAATTY 9 cut(s) 104, 230, 273, 613, 682, 1143, 1380, 2329, 3650
ArsI GACNNNNNNTTYG 8 cut(s) 425, 457, 1183, 1215, 3589, 3621, 3819, 3851
AseI ATTAAT 1 cut(s) 3459
Asp700I GAANNNNTTC 3 cut(s) 309, 1315, 2329
AspLEI GCGC 2 cut(s) 1881, 3922
AspS9I GGNCC 5 cut(s) 1760, 2505, 2600, 2690, 2972
AsuC2I CCSGG 1 cut(s) 4043
AsuII TTCGAA 1 cut(s) 3524
AvaII GGWCC 4 cut(s) 1760, 2505, 2600, 2690
BaeGI GKGCMC 2 cut(s) 1189, 3790
BalI TGGCCA 2 cut(s) 594, 742
BamHI GGATCC 4 cut(s) 649, 1256, 3099, 3343
BanI GGYRCC 2 cut(s) 1337, 2609
BanII GRGCYC 1 cut(s) 1897
BbrPI CACGTG 1 cut(s) 449
BbsI GAAGAC 6 cut(s) 76, 3150, 3180, 3274, 3765, 3834
Bbv12I GWGCWC 6 cut(s) 1189, 2420, 3541, 3790, 3884, 3971
BbvI GCAGC 9 cut(s) 805, 851, 1343, 2048, 2380, 2459, 2462, 3696, 3791
BceAI ACGGC 1 cut(s) 4043
BcgI CGANNNNNNTGC 2 cut(s) 2357, 2391
BciT130I CCWGG 9 cut(s) 900, 1101, 2085, 2965, 2976, 3063, 3123, 3179, 3219
BclI TGATCA 1 cut(s) 2800
BcnI CCSGG 1 cut(s) 4043
BcoDI GTCTC 7 cut(s) 564, 767, 1727, 2219, 3144, 3611, 3841
BcuI ACTAGT 2 cut(s) 517, 1939
BfmI CTRYAG 4 cut(s) 546, 819, 1947, 3982
BfuAI ACCTGC 1 cut(s) 3773
BglI GCCNNNNNGGC 2 cut(s) 2459, 3284
BglII AGATCT 2 cut(s) 1970, 3716
BlpI GCTNAGC 2 cut(s) 324, 1729
Bme18I GGWCC 4 cut(s) 1760, 2505, 2600, 2690
BmgT120I GGNCC 5 cut(s) 1760, 2505, 2600, 2690, 2972
BmrI ACTGGG 2 cut(s) 93, 1525
BmuI ACTGGG 2 cut(s) 93, 1525
BpiI GAAGAC 6 cut(s) 76, 3150, 3180, 3274, 3765, 3834
BplI GAGNNNNNCTC 4 cut(s) 2156, 2188, 3978, 4010
BpmI CTGGAG 7 cut(s) 809, 981, 1761, 2511, 2687, 3045, 3497
Bpu10I CCTNAGC 1 cut(s) 2695
Bpu1102I GCTNAGC 2 cut(s) 324, 1729
Bpu14I TTCGAA 1 cut(s) 3524
BpuEI CTTGAG 3 cut(s) 1881, 2039, 2763
BpuMI CCSGG 1 cut(s) 4043
Bsa29I ATCGAT 1 cut(s) 3619
BsaAI YACGTR 1 cut(s) 449
BsaBI GATNNNNATC 1 cut(s) 3070
BsaI GGTCTC 2 cut(s) 564, 3611
BsaJI CCNNGG 4 cut(s) 1100, 2964, 3178, 4041
BsaWI WCCGGW 1 cut(s) 2408
BsaXI ACNNNNNCTCC 4 cut(s) 2484, 2514, 4052, 4082
Bsc4I CCNNNNNNNGG 7 cut(s) 505, 2970, 2981, 3205, 3310, 4022, 4042
Bse118I RCCGGY 1 cut(s) 3555
Bse3DI GCAATG 5 cut(s) 105, 341, 459, 1476, 3679
Bse8I GATNNNNATC 1 cut(s) 3070
BseBI CCWGG 9 cut(s) 900, 1101, 2085, 2965, 2976, 3063, 3123, 3179, 3219
BseCI ATCGAT 1 cut(s) 3619
BseDI CCNNGG 4 cut(s) 1100, 2964, 3178, 4041
BseJI GATNNNNATC 1 cut(s) 3070
BseLI CCNNNNNNNGG 7 cut(s) 505, 2970, 2981, 3205, 3310, 4022, 4042
BseMI GCAATG 5 cut(s) 105, 341, 459, 1476, 3679
BseMII CTCAG 6 cut(s) 1467, 2669, 3099, 3744, 3897, 3996
BseRI GAGGAG 5 cut(s) 1106, 1920, 2766, 2909, 3858
BseSI GKGCMC 2 cut(s) 1189, 3790
BseXI GCAGC 9 cut(s) 805, 851, 1343, 2048, 2380, 2459, 2462, 3696, 3791
BseYI CCCAGC 1 cut(s) 3726
BsgI GTGCAG 1 cut(s) 2466
Bsh1236I CGCG 1 cut(s) 1777
Bsh1285I CGRYCG 1 cut(s) 3373
BshFI GGCC 6 cut(s) 594, 742, 2100, 2672, 2974, 3278
BshNI GGYRCC 2 cut(s) 1337, 2609
BshVI ATCGAT 1 cut(s) 3619
BsiEI CGRYCG 1 cut(s) 3373
BsiHKAI GWGCWC 6 cut(s) 1189, 2420, 3541, 3790, 3884, 3971
BsiSI CCGG 3 cut(s) 2409, 3556, 4043
BslFI GGGAC 1 cut(s) 2464
BslI CCNNNNNNNGG 7 cut(s) 505, 2970, 2981, 3205, 3310, 4022, 4042
BsmAI GTCTC 7 cut(s) 564, 767, 1727, 2219, 3144, 3611, 3841
BsmFI GGGAC 1 cut(s) 2464
BsmI GAATGC 4 cut(s) 2088, 2275, 2567, 3061
BsnI GGCC 6 cut(s) 594, 742, 2100, 2672, 2974, 3278
Bso31I GGTCTC 2 cut(s) 564, 3611
Bsp119I TTCGAA 1 cut(s) 3524
Bsp1286I GDGCHC 7 cut(s) 1189, 1897, 2420, 3541, 3790, 3884, 3971
Bsp1407I TGTACA 1 cut(s) 501
Bsp1720I GCTNAGC 2 cut(s) 324, 1729
BspACI CCGC 8 cut(s) 364, 1865, 3678, 3712, 3805, 3977, 4033, 4071
BspANI GGCC 6 cut(s) 594, 742, 2100, 2672, 2974, 3278
BspCNI CTCAG 6 cut(s) 1466, 2670, 3098, 3745, 3896, 3997
BspDI ATCGAT 1 cut(s) 3619
BspFNI CGCG 1 cut(s) 1777
BspMAI CTGCAG 3 cut(s) 550, 823, 3986
BspMI ACCTGC 1 cut(s) 3773
BspQI GCTCTTC 2 cut(s) 2234, 3699
BspT104I TTCGAA 1 cut(s) 3524
BspT107I GGYRCC 2 cut(s) 1337, 2609
BspTNI GGTCTC 2 cut(s) 564, 3611
BsrDI GCAATG 5 cut(s) 105, 341, 459, 1476, 3679
BsrFI RCCGGY 1 cut(s) 3555
BsrGI TGTACA 1 cut(s) 501
BssAI RCCGGY 1 cut(s) 3555
BssECI CCNNGG 4 cut(s) 1100, 2964, 3178, 4041
BssNAI GTATAC 1 cut(s) 61
Bst1107I GTATAC 1 cut(s) 61
Bst2UI CCWGG 9 cut(s) 900, 1101, 2085, 2965, 2976, 3063, 3123, 3179, 3219
Bst6I CTCTTC 3 cut(s) 1570, 2234, 3699
BstAPI GCANNNNNTGC 5 cut(s) 1337, 2300, 3552, 3785, 3859
BstAUI TGTACA 1 cut(s) 501
BstBAI YACGTR 1 cut(s) 449
BstBI TTCGAA 1 cut(s) 3524
BstEII GGTNACC 1 cut(s) 3299
BstENI CCTNNNNNAGG 2 cut(s) 2979, 3203
BstFNI CGCG 1 cut(s) 1777
BstHHI GCGC 2 cut(s) 1881, 3922
BstMAI GTCTC 7 cut(s) 564, 767, 1727, 2219, 3144, 3611, 3841
BstMCI CGRYCG 1 cut(s) 3373
BstNI CCWGG 9 cut(s) 900, 1101, 2085, 2965, 2976, 3063, 3123, 3179, 3219
BstNSI RCATGY 3 cut(s) 1226, 1797, 2944
BstPI GGTNACC 1 cut(s) 3299
BstSFI CTRYAG 4 cut(s) 546, 819, 1947, 3982
BstSLI GKGCMC 2 cut(s) 1189, 3790
BstUI CGCG 1 cut(s) 1777
BstV1I GCAGC 9 cut(s) 805, 851, 1343, 2048, 2380, 2459, 2462, 3696, 3791
BstV2I GAAGAC 6 cut(s) 76, 3150, 3180, 3274, 3765, 3834
BstX2I RGATCY 8 cut(s) 649, 1256, 1970, 2209, 3099, 3343, 3508, 3716
BstXI CCANNNNNNTGG 2 cut(s) 980, 2266
BstYI RGATCY 8 cut(s) 649, 1256, 1970, 2209, 3099, 3343, 3508, 3716
BstZ17I GTATAC 1 cut(s) 61
Bsu15I ATCGAT 1 cut(s) 3619
BsuRI GGCC 6 cut(s) 594, 742, 2100, 2672, 2974, 3278
BsuTUI ATCGAT 1 cut(s) 3619
BtsI GCAGTG 5 cut(s) 543, 1564, 1674, 2532, 3541
BveI ACCTGC 1 cut(s) 3773
CaiI CAGNNNCTG 4 cut(s) 1515, 1636, 2893, 3080
CfoI GCGC 2 cut(s) 1881, 3922
Cfr10I RCCGGY 1 cut(s) 3555
Cfr13I GGNCC 5 cut(s) 1760, 2505, 2600, 2690, 2972
ClaI ATCGAT 1 cut(s) 3619
CseI GACGC 1 cut(s) 81
CsiI ACCWGGT 1 cut(s) 3121
Csp6I GTAC 5 cut(s) 492, 502, 521, 1173, 3642
CspCI CAANNNNNGTGG 2 cut(s) 1384, 1419
CviQI GTAC 5 cut(s) 492, 502, 521, 1173, 3642
DraI TTTAAA 2 cut(s) 688, 2313
DraIII CACNNNGTG 4 cut(s) 260, 2033, 2558, 3125
EaeI YGGCCR 2 cut(s) 592, 740
Eam1104I CTCTTC 3 cut(s) 1570, 2234, 3699
EarI CTCTTC 3 cut(s) 1570, 2234, 3699
EciI GGCGGA 3 cut(s) 3966, 4022, 4060
Eco24I GRGCYC 1 cut(s) 1897
Eco31I GGTCTC 2 cut(s) 564, 3611
Eco32I GATATC 2 cut(s) 139, 2934
Eco47I GGWCC 4 cut(s) 1760, 2505, 2600, 2690
Eco57I CTGAAG 4 cut(s) 719, 801, 990, 3884
Eco72I CACGTG 1 cut(s) 449
Eco91I GGTNACC 1 cut(s) 3299
EcoNI CCTNNNNNAGG 2 cut(s) 2979, 3203
EcoO109I RGGNCCY 4 cut(s) 1760, 2505, 2600, 2690
EcoO65I GGTNACC 1 cut(s) 3299
EcoRI GAATTC 1 cut(s) 230
EcoRII CCWGG 9 cut(s) 898, 1099, 2083, 2963, 2974, 3061, 3121, 3177, 3217
EcoRV GATATC 2 cut(s) 139, 2934
EcoT22I ATGCAT 3 cut(s) 1570, 1799, 2946
EcoT38I GRGCYC 1 cut(s) 1897
FalI AAGNNNNNCTT 6 cut(s) 916, 948, 1272, 1304, 2796, 2828
FaqI GGGAC 1 cut(s) 2464
FbaI TGATCA 1 cut(s) 2800
FblI GTMKAC 2 cut(s) 60, 1473
FriOI GRGCYC 1 cut(s) 1897
FspI TGCGCA 1 cut(s) 1880
GlaI GCGC 2 cut(s) 1880, 3921
GsaI CCCAGC 1 cut(s) 3730
GsuI CTGGAG 7 cut(s) 809, 981, 1761, 2511, 2687, 3045, 3497
HaeIII GGCC 6 cut(s) 594, 742, 2100, 2672, 2974, 3278
HapII CCGG 3 cut(s) 2409, 3556, 4043
HgaI GACGC 1 cut(s) 81
HhaI GCGC 2 cut(s) 1881, 3922
Hin6I GCGC 2 cut(s) 1879, 3920
HinP1I GCGC 2 cut(s) 1879, 3920
HincII GTYRAC 3 cut(s) 991, 1617, 2012
HindII GTYRAC 3 cut(s) 991, 1617, 2012
HpaI GTTAAC 1 cut(s) 1617
HpaII CCGG 3 cut(s) 2409, 3556, 4043
HpyCH4IV ACGT 4 cut(s) 448, 908, 2014, 3640
HpySE526I ACGT 4 cut(s) 448, 908, 2014, 3640
HspAI GCGC 2 cut(s) 1879, 3920
KroI GCCGGC 1 cut(s) 3555
KroNI GCCGGC 1 cut(s) 3557
Ksp22I TGATCA 1 cut(s) 2800
KspAI GTTAAC 1 cut(s) 1617
LguI GCTCTTC 2 cut(s) 2234, 3699
LmnI GCTCC 8 cut(s) 514, 1742, 2492, 2870, 3064, 3569, 3845, 4035
Lsp1109I GCAGC 9 cut(s) 805, 851, 1343, 2048, 2380, 2459, 2462, 3696, 3791
MabI ACCWGGT 1 cut(s) 3121
MaeII ACGT 4 cut(s) 448, 908, 2014, 3640
MaeIII GTNAC 9 cut(s) 241, 377, 431, 902, 1352, 1951, 2119, 3299, 3927
MfeI CAATTG 4 cut(s) 318, 329, 2220, 4053
MflI RGATCY 8 cut(s) 649, 1256, 1970, 2209, 3099, 3343, 3508, 3716
MhlI GDGCHC 7 cut(s) 1189, 1897, 2420, 3541, 3790, 3884, 3971
MlsI TGGCCA 2 cut(s) 594, 742
MluNI TGGCCA 2 cut(s) 594, 742
MlyI GAGTC 3 cut(s) 1055, 1598, 3935
MmeI TCCRAC 3 cut(s) 3190, 3321, 3837
Mox20I TGGCCA 2 cut(s) 594, 742
Mph1103I ATGCAT 3 cut(s) 1570, 1799, 2946
MroNI GCCGGC 1 cut(s) 3555
MroXI GAANNNNTTC 3 cut(s) 309, 1315, 2329
MscI TGGCCA 2 cut(s) 594, 742
MslI CAYNNNNRTG 1 cut(s) 200
Msp20I TGGCCA 2 cut(s) 594, 742
MspA1I CMGCKG 1 cut(s) 1334
MspI CCGG 3 cut(s) 2409, 3556, 4043
MunI CAATTG 4 cut(s) 318, 329, 2220, 4053
Mva1269I GAATGC 4 cut(s) 2088, 2275, 2567, 3061
MvaI CCWGG 9 cut(s) 900, 1101, 2085, 2965, 2976, 3063, 3123, 3179, 3219
MvnI CGCG 1 cut(s) 1777
NaeI GCCGGC 1 cut(s) 3557
NciI CCSGG 1 cut(s) 4043
NgoMIV GCCGGC 1 cut(s) 3555
NmuCI GTSAC 6 cut(s) 241, 377, 431, 1352, 3299, 3927
NsbI TGCGCA 1 cut(s) 1880
NsiI ATGCAT 3 cut(s) 1570, 1799, 2946
NspI RCATGY 3 cut(s) 1226, 1797, 2944
NspV TTCGAA 1 cut(s) 3524
PaeI GCATGC 1 cut(s) 1797
PaqCI CACCTGC 1 cut(s) 3773
PciI ACATGT 1 cut(s) 1222
PciSI GCTCTTC 2 cut(s) 2234, 3699
PctI GAATGC 4 cut(s) 2088, 2275, 2567, 3061
PdiI GCCGGC 1 cut(s) 3557
PdmI GAANNNNTTC 3 cut(s) 309, 1315, 2329
PflFI GACNNNGTC 1 cut(s) 3833
PfoI TCCNGGA 1 cut(s) 3061
Ple19I CGATCG 1 cut(s) 3373
PleI GAGTC 3 cut(s) 1055, 1597, 3934
PmaCI CACGTG 1 cut(s) 449
PmlI CACGTG 1 cut(s) 449
PpsI GAGTC 3 cut(s) 1055, 1597, 3934
Ppu21I YACGTR 1 cut(s) 449
PpuMI RGGWCCY 4 cut(s) 1760, 2505, 2600, 2690
PscI ACATGT 1 cut(s) 1222
PshBI ATTAAT 1 cut(s) 3459
Psp5II RGGWCCY 4 cut(s) 1760, 2505, 2600, 2690
Psp6I CCWGG 9 cut(s) 898, 1099, 2083, 2963, 2974, 3061, 3121, 3177, 3217
PspCI CACGTG 1 cut(s) 449
PspEI GGTNACC 1 cut(s) 3299
PspFI CCCAGC 1 cut(s) 3726
PspGI CCWGG 9 cut(s) 898, 1099, 2083, 2963, 2974, 3061, 3121, 3177, 3217
PspPI GGNCC 5 cut(s) 1760, 2505, 2600, 2690, 2972
PspPPI RGGWCCY 4 cut(s) 1760, 2505, 2600, 2690
PstI CTGCAG 3 cut(s) 550, 823, 3986
PstNI CAGNNNCTG 4 cut(s) 1515, 1636, 2893, 3080
PsuI RGATCY 8 cut(s) 649, 1256, 1970, 2209, 3099, 3343, 3508, 3716
PsyI GACNNNGTC 1 cut(s) 3833
PvuI CGATCG 1 cut(s) 3373
PvuII CAGCTG 1 cut(s) 1334
RsaI GTAC 5 cut(s) 493, 503, 522, 1174, 3643
RsaNI GTAC 5 cut(s) 492, 502, 521, 1173, 3642
RseI CAYNNNNRTG 1 cut(s) 200
SapI GCTCTTC 2 cut(s) 2234, 3699
Sau96I GGNCC 5 cut(s) 1760, 2505, 2600, 2690, 2972
SchI GAGTC 3 cut(s) 1055, 1598, 3935
SduI GDGCHC 7 cut(s) 1189, 1897, 2420, 3541, 3790, 3884, 3971
SexAI ACCWGGT 1 cut(s) 3121
SfcI CTRYAG 4 cut(s) 546, 819, 1947, 3982
SfuI TTCGAA 1 cut(s) 3524
SinI GGWCC 4 cut(s) 1760, 2505, 2600, 2690
SmiMI CAYNNNNRTG 1 cut(s) 200
SmlI CTYRAG 3 cut(s) 1896, 2018, 2778
SmoI CTYRAG 3 cut(s) 1896, 2018, 2778
SpeI ACTAGT 2 cut(s) 517, 1939
SphI GCATGC 1 cut(s) 1797
SsiI CCGC 8 cut(s) 364, 1865, 3678, 3712, 3805, 3977, 4033, 4071
TaiI ACGT 4 cut(s) 451, 911, 2017, 3643
TaqI TCGA 9 cut(s) 1149, 1838, 1903, 2360, 2377, 3369, 3463, 3524, 3619
TatI WGTACW 1 cut(s) 501
TauI GCSGC 1 cut(s) 3680
TseFI GTSAC 6 cut(s) 241, 377, 431, 1352, 3299, 3927
TseI GCWGC 9 cut(s) 818, 839, 1331, 2061, 2368, 2447, 2450, 3709, 3779
Tsp45I GTSAC 6 cut(s) 241, 377, 431, 1352, 3299, 3927
TspGWI ACGGA 2 cut(s) 3661, 3986
Tth111I GACNNNGTC 1 cut(s) 3833
VneI GTGCAC 2 cut(s) 1185, 3786
VpaK11BI GGWCC 4 cut(s) 1760, 2505, 2600, 2690
VspI ATTAAT 1 cut(s) 3459
XagI CCTNNNNNAGG 2 cut(s) 2979, 3203
XapI RAATTY 9 cut(s) 104, 230, 273, 613, 682, 1143, 1380, 2329, 3650
XbaI TCTAGA 2 cut(s) 3238, 3839
XceI RCATGY 3 cut(s) 1226, 1797, 2944
XcmI CCANNNNNNNNNTGG 3 cut(s) 875, 1402, 2461
XmiI GTMKAC 2 cut(s) 60, 1473
XmnI GAANNNNTTC 3 cut(s) 309, 1315, 2329
Zsp2I ATGCAT 3 cut(s) 1570, 1799, 2946
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.