MD07G1306200.v1.1

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0004502
Physical Location & Seq
Forward (+)
36273968 .. 36282408
8441 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1306200.v1.1.491

Sequence Viewer

Length: 4002 bp
ATGGGAGTCGAGAACTACCATGTGATTGAGCTTGTAGGTGAAGGTTCCTTTGGGAAGGTATACAAGGGAAGGCGAAAGCACACCGGTCAGACCGTTGCAATGAAATTCATCATGAAGCATGGCAAAAGTGACAAAGATATCCACAATTTAAGACAAGAAATTGAGATCCTACGAAAGTTGAAGCACGAAAACATCATTGAAATGCTTGATTCCTTTGAAAGCCCACAAGAGTTCTGTGTTGTCACAGAATTTGCACAAGGTGAACTATTTGAAATTCTTGAGGATGATAAGTGCCTTCCTGAAGAACAAGTTCAAGCTATTGCAAAGCAGCTGGTGAGGGCATTGCATTATCTGCATTCCAACCGTATCATCCATCGTGACATGAAACCACAAAACATTCTCATTGGTGCTGGTTCTATTGTTAAGCTCTGTGATTTTGGGTTTGCACGTGCAATGTCCACAAACACTGTTGTTTTGCGATCCATTAAAGGTACTCCTCTGTACATGGCACCTGAACTAGTACGGGAACAACCTTACAACCACACTGCAGATCTCTGGTCTCTTGGAGTAATATTATATGAATTATTTGTAGGCCAGCCTCCCTTTTATACAAACTCTGTATATGCACTCATCAGGCACATTGTCAAGGATCCAGTTAAATATCCTGACAACATGAGTCCAAGTTTTAAAAACTTTCTTAAGGGACTGCTCAATAAGGTACCTCAAAATCGATTGACTTGGCCAGCTCTTCTGGAACACCCATTTGTTAAAGAGACTCCTCATGAACTGGAGGCTAGGGAAATGCGTTCAGCTACTGCTGCAGATAGGGGATGTGTTGCGGCATGGAGGGGTGAAGGAAACAAAGTTCAAACATCAGTTGTGGCTGTTTCATCTCCTGCTTCTTCAGAGAACAACAGTGGAATAAGTTTTCAGAATGATGCTCAAGCAAATACTCCTGAATCCACAACAGTCAATTCTTCCCCAAATGAGTTCCCAGGATTTGCAAATCCTAATGAAGTTAAACAGTCAGGTTTCCAAATTTTGGACAGATTGGAAAATAACTCTCGTACAGTCAAAGGTGCACAAATAATAAGTCAAGATAATGAAGCATTAGCACATGTTTTGGTACCACTAAAAAGATGCTCCAATGGACCCCCAAATTCTTGCAAGGATGAAGATATTATTAATTCAAACCAGTCTTTGAGAATCCTTTCAAACTTAGTTGCAGCTGGTGCCTTCCAGTCCAGCGGACTGCTTGATGAAATAATACACGAACTTCTTGCTTACACTGCCATCATTGTGAGCATGAAATCTTCTGAAGTAAATGAGTTAAAGGCGAAGAGTTTCTCAGTGATTAAAATTTTGGTTGACAATGCTGGAAGCAGCATTGGTGGCTCATATTTCAGGCACTGGGTTGCCTTATCTGATATTTTTTCACAGGTTGTGGATTGTAGTGAAGATGCATCTGGAAGAGTTCTGTATGAGTCCATTGCTTGCATTACTATCATGTTAAGAAGAGTCACTCAGGGCCTTAAATCTTTTTCCTCAACCTCTGATCCTAATGAAGCACTGAAACAGATTCTGGATCACGCTAAAAGATCTGGTTTAGTGGACCAACTTTGTCTCTGCTTAGTAACCGCAGGGTCAAGTCTTATTTCAGGCTCTTCAAATATGCTGCGTGCAGCTTGTGAAGCATGTGTGGCAATTTGGTTTCTGATAGATGCATCAGAAAATCTTTGTATGAAAAGAAATGCCTATTTATTCCCGCTAAACACTATGCGCCGGCCTTCACCCCAACTTGACATTAGGGATCAAGATCAAGGTTCTTTGATTGGGACAGAAGCATCAAAACTTGTTGCTGTAGTTACAAGAGCATTCCTTAGATCAAAAGCAGTACAAGTTGCTATCCATTATTGCCTTCACCAACGACTTGAAGCTTCACTGTATGCTGTCATTCAGTTGTTGTTGAGGTGCTGCTTGCATAATGGAATTGTTCCAGGTGTTCTATGTGGCCTGCCTACTTCCCTACCTGTAACTACTGTTGTTAGTGGCGGAGGTGATGGAACCATTATTTCAGAGATATTCTCTTTGTTGTCTTTGTGCATTTCGTCTCAAAATAAAGATCCCCAAGCAGTTGAGACAACCACCTTCATGAGCAAATTAACCAATCCCACTACCTTGGTTCTGCATTCATGCCTCATCCTTGCAACAGTTGCACAATGTTTGAAGGCAACTGGAAGAAATTCTGCACTATTTATGCTAACGACCTCTCCAAAGAAGCAGCTTTCTCGACTTTCTGTCCTTTCTCATCATTTTTCTTCTGAAGAGAGCAAAAATGCCTCCTTTCAAGCTCATACTTCATCAGCCATGCTGGCACTGGCTTCTATTTTGTTCCTTGAATCTGGCAGTTCTGTTGAGTCTTCTATCTTTGAGGTAGCAGTGCCCTTGATTCCTAAAAGTGCCATGCTATGTGAATACCTGAAGCTTTCATCAGGCAAAGGAAATGAATTGCGTTTGAATGGCCCCAGTGGTGCTCTCTCATACTGGCATGGTCTAAGGGATGGATGTGTTGGCTTGCTGGAGTCCAGACTGAGGTGGGGAGGACCCTTAGCTGTTAAACAGCTGTGTGCAAGCAATATCCCTCTGCTTCTAGTGGATTTGTTAGCCAACAACCAACCAGAAGTTGACAGCACCAATGATCAAGTTGGGCTTTCCCCTATAGGAGTCGTATGGACAGTATTGTCAATATGTCAGTGCCTTTCCGGTGGAGCCTTAACTTTTCGGCAGATTCTGCTTAGATGTAATCATATCAAGCTCATCTCTGACTTGATATCTGATATGCATCTCAAGCTTGTAAAGTCCTGGGTTGGGCCTGGTGGAGGGAAGGATGGTGTAAGAGATATAACAAACGCAGTAATTGATGTCTTGGCATTTCCTTTTGTGGCCGTACAGAATGTTCCAGGATTGCCATCAACCACTGCTTCAGTGAATAGTGGGGCCCTTCTCAACCTGGGTTCACCAGGTGTGAGAGTTGGCATGGAAGACAAAGACATGGTGAAAGTAATTGAAGAAGACCTTGGAAAGTATATTAAAAACCTTTTGGAGGTAGGAGTGCCAGGCATCATTCTTTGGTGTTTAGAGCACTTGGACTTAAAGGATATGGGAAGGCCTGTGGCCTTCCTTGCAAAAATGATTGGTTACCGACCATTGGCAGTTCAACTTGTGGGTAAAGGCTTGTTGGATCCTAAAAGGATGAGAAGATTGCTCGACTGTTCAAGCCCAAGAGAGGTGATGCTGGATGTTCTGATGATTGTTTCTGATTTGGCTCGCATGGACAAGGGATTCTACGAATACATTAATGGAGCATCTGTATTGGAGTTCTTCAAGGAATTTCTTACCCATGAAGATCCTAATGTGCGCTCAAAGGCTTGCAGTGCTCTAGGAAATATGTGCCGGCACAGCTCCTACTTTTATAGTTCCCTAGCAAGGCATCAAATCATTGGTCTCCTTATTGATCGATGTTCTGATCCAGATAAACGGACACGGAAATTTGCTTGCTTTGCTATTGGGAATGCTGCCTACCATGACAAGATATTATATGAAGAGCTACGAAGATCTATACCCTCTCTTGCGAATTTGTTGGTTTCGTCCGAAGAAGACAAGACTAAAGCAAATGCTGCAGGTGCACTAAGCAATCTCGTCCGCCACTCCGACCAGCTTTGCGAAGATATTGTGTCTAAGGGAGCCATGCAGTCTCTACTGAAGTTGGTGGCTGATTGTTCTGTGGTGGCACTCAACCCGCCGGGTAGGAAAGATTCAGTAAACGAGTCACCTCTGAAAATAGCTCTGTTCTCATTGGCAAAGATGTGTTCGCATCAGCCCTGCAGACAATTCCTCCGTTCATCACCGCTGTTCTCTGTGATCGGACGCCTTCGGCAATCTCCGGAATCAACAATTGCCAACTATGCCTCCCTTATTTTCACCAAAGTTGCTGATTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000166 GO:0000278 GO:0000280 GO:0000281 GO:0000910 GO:0000911 GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005856 GO:0005874 GO:0005881 GO:0006355 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006935 GO:0006996 GO:0007049 GO:0007112 GO:0007140 GO:0007154 GO:0007163 GO:0007165 GO:0007166 GO:0007224 GO:0007227 GO:0007228 GO:0007275 GO:0007276 GO:0007349 GO:0007584 GO:0008064 GO:0008092 GO:0008134 GO:0008144 GO:0008150 GO:0008152 GO:0008589 GO:0009524 GO:0009553 GO:0009558 GO:0009561 GO:0009605 GO:0009653 GO:0009791 GO:0009889 GO:0009966 GO:0009967 GO:0009987 GO:0009991 GO:0010033 GO:0010243 GO:0010468 GO:0010556 GO:0010639 GO:0010646 GO:0010647 GO:0014070 GO:0015630 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017076 GO:0019219 GO:0019222 GO:0019538 GO:0019894 GO:0019953 GO:0022402 GO:0022412 GO:0022414 GO:0022607 GO:0023051 GO:0023052 GO:0023056 GO:0030010 GO:0030030 GO:0030031 GO:0030554 GO:0030832 GO:0030833 GO:0030837 GO:0031156 GO:0031272 GO:0031323 GO:0031326 GO:0031333 GO:0031344 GO:0031667 GO:0032101 GO:0032104 GO:0032107 GO:0032271 GO:0032272 GO:0032501 GO:0032502 GO:0032504 GO:0032506 GO:0032535 GO:0032553 GO:0032555 GO:0032559 GO:0032956 GO:0032970 GO:0033043 GO:0033206 GO:0033273 GO:0035556 GO:0035639 GO:0036094 GO:0036211 GO:0040011 GO:0042221 GO:0042330 GO:0042493 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043254 GO:0043326 GO:0043327 GO:0043412 GO:0043900 GO:0044085 GO:0044087 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0044703 GO:0044782 GO:0045880 GO:0048229 GO:0048232 GO:0048285 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048609 GO:0048646 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051090 GO:0051091 GO:0051128 GO:0051129 GO:0051171 GO:0051252 GO:0051301 GO:0051321 GO:0051493 GO:0051494 GO:0051593 GO:0051704 GO:0051716 GO:0060176 GO:0060255 GO:0060271 GO:0060491 GO:0061640 GO:0065007 GO:0065008 GO:0065009 GO:0070925 GO:0071704 GO:0071840 GO:0080090 GO:0080134 GO:0080135 GO:0090066 GO:0097159 GO:0097367 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0110053 GO:0120031 GO:0120032 GO:0120035 GO:0120036 GO:0140013 GO:0140096 GO:1901265 GO:1901363 GO:1901564 GO:1901698 GO:1901700 GO:1902410 GO:1902903 GO:1902904 GO:1903046 GO:1903047 GO:1903506 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04341 map04341
Pfam Domains
Protein Families
Arm HEAT HEAT_2 Pkinase

Protein Analysis

1334

Amino Acids

145.97

Weight (kDa)

8.11

Isoelectric Point (pI)

47.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 6 - 256 2.1e-79 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 8 - 251 5.6e-47 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 3671
AasI GACNNNNNNGTC 1 cut(s) 2740
Acc36I ACCTGC 1 cut(s) 3671
Acc65I GGTACC 2 cut(s) 718, 1126
AccB1I GGYRCC 4 cut(s) 508, 718, 1126, 1232
AccB7I CCANNNNNTGG 1 cut(s) 1042
AccI GTMKAC 1 cut(s) 60
AccIII TCCGGA 1 cut(s) 3943
AciI CCGC 8 cut(s) 839, 1248, 1638, 1766, 2052, 3703, 3800, 3908
AcoI YGGCCR 2 cut(s) 740, 2943
AcuI CTGAAG 7 cut(s) 321, 888, 1338, 2343, 2501, 2967, 3782
AcvI CACGTG 1 cut(s) 449
AcyI GRCGYC 1 cut(s) 3928
AdeI CACNNNGTG 2 cut(s) 260, 3023
AfaI GTAC 8 cut(s) 493, 503, 522, 720, 1069, 1128, 1896, 2949
AfiI CCNNNNNNNGG 8 cut(s) 1042, 2592, 2868, 2879, 3103, 3208, 3286, 3486
AflII CTTAAG 1 cut(s) 698
AflIII ACRYGT 1 cut(s) 1117
AgeI ACCGGT 1 cut(s) 83
AhdI GACNNNNNGTC 1 cut(s) 2297
AhlI ACTAGT 1 cut(s) 517
AjnI CCWGG 8 cut(s) 994, 1996, 2861, 2872, 2959, 3009, 3019, 3115
AjuI GAANNNNNNNTTGG 4 cut(s) 33, 65, 3060, 3092
AloI GAACNNNNNNTCC 2 cut(s) 849, 881
Alw21I GWGCWC 5 cut(s) 1084, 2536, 3144, 3439, 3688
Alw26I GTCTC 7 cut(s) 564, 767, 1628, 2115, 2132, 3509, 3759
Alw44I GTGCAC 2 cut(s) 1080, 3684
AlwNI CAGNNNCTG 4 cut(s) 815, 1410, 1582, 2791
Aor13HI TCCGGA 1 cut(s) 3943
ApaI GGGCCC 1 cut(s) 3001
ApaLI GTGCAC 2 cut(s) 1080, 3684
ArsI GACNNNNNNTTYG 4 cut(s) 1078, 1110, 3487, 3519
AseI ATTAAT 2 cut(s) 1185, 3357
AsiGI ACCGGT 1 cut(s) 83
Asp700I GAANNNNTTC 4 cut(s) 309, 1210, 1343, 2242
Asp718I GGTACC 2 cut(s) 718, 1126
AspLEI GCGC 2 cut(s) 1782, 3420
AspS9I GGNCC 8 cut(s) 1151, 1528, 1612, 2522, 2603, 2870, 2997, 2998
AsuC2I CCSGG 1 cut(s) 3804
AvaII GGWCC 3 cut(s) 1151, 1612, 2603
BaeGI GKGCMC 4 cut(s) 1084, 2445, 3001, 3688
BalI TGGCCA 1 cut(s) 742
BamHI GGATCC 2 cut(s) 649, 3241
BanI GGYRCC 4 cut(s) 508, 718, 1126, 1232
BanII GRGCYC 1 cut(s) 3001
BbrPI CACGTG 1 cut(s) 449
BbsI GAAGAC 4 cut(s) 2412, 3048, 3078, 3663
Bbv12I GWGCWC 5 cut(s) 1084, 2536, 3144, 3439, 3688
BccI CCATC 6 cut(s) 381, 1301, 2054, 2555, 2882, 2977
BceAI ACGGC 1 cut(s) 2930
BcgI CGANNNNNNTGC 4 cut(s) 1262, 1296, 2270, 2304
BciT130I CCWGG 8 cut(s) 996, 1998, 2863, 2874, 2961, 3011, 3021, 3117
BclI TGATCA 1 cut(s) 2698
BcnI CCSGG 1 cut(s) 3804
BcoDI GTCTC 7 cut(s) 564, 767, 1628, 2115, 2132, 3509, 3759
BcuI ACTAGT 1 cut(s) 517
BfaI CTAG 5 cut(s) 518, 795, 2651, 3440, 3482
BfmI CTRYAG 6 cut(s) 546, 819, 1860, 2718, 3678, 3883
BfrI CTTAAG 1 cut(s) 698
BfuAI ACCTGC 1 cut(s) 3671
BglI GCCNNNNNGGC 1 cut(s) 2372
BglII AGATCT 3 cut(s) 550, 1598, 3614
Bme1390I CCNGG 9 cut(s) 996, 1998, 2863, 2874, 2961, 3011, 3021, 3117, 3804
Bme18I GGWCC 3 cut(s) 1151, 1612, 2603
BmeRI GACNNNNNGTC 1 cut(s) 2297
BmgT120I GGNCC 8 cut(s) 1151, 1528, 1612, 2522, 2603, 2870, 2997, 2998
BmrFI CCNGG 9 cut(s) 996, 1998, 2863, 2874, 2961, 3011, 3021, 3117, 3804
BmrI ACTGGG 2 cut(s) 1420, 2520
BmuI ACTGGG 2 cut(s) 1420, 2520
BpiI GAAGAC 4 cut(s) 2412, 3048, 3078, 3663
BplI GAGNNNNNCTC 2 cut(s) 2069, 2101
BpmI CTGGAG 2 cut(s) 809, 2600
Bpu10I CCTNAGC 1 cut(s) 2608
BpuEI CTTGAG 3 cut(s) 299, 927, 2831
BpuMI CCSGG 1 cut(s) 3804
Bsa29I ATCGAT 2 cut(s) 730, 3517
BsaAI YACGTR 1 cut(s) 449
BsaBI GATNNNNATC 3 cut(s) 1815, 2841, 2968
BsaHI GRCGYC 1 cut(s) 3928
BsaI GGTCTC 2 cut(s) 564, 3509
BsaJI CCNNGG 5 cut(s) 994, 2178, 2862, 3010, 3076
BsaWI WCCGGW 3 cut(s) 83, 2762, 3943
BsaXI ACNNNNNCTCC 8 cut(s) 2254, 2284, 3354, 3384, 3879, 3909, 3953, 3983
Bsc4I CCNNNNNNNGG 8 cut(s) 1042, 2592, 2868, 2879, 3103, 3208, 3286, 3486
Bse118I RCCGGY 3 cut(s) 83, 1782, 3453
Bse1I ACTGG 9 cut(s) 653, 792, 1195, 1240, 1415, 2239, 2382, 2526, 2549
Bse3DI GCAATG 4 cut(s) 105, 341, 459, 1488
Bse8I GATNNNNATC 3 cut(s) 1815, 2841, 2968
BseAI TCCGGA 1 cut(s) 3943
BseBI CCWGG 8 cut(s) 996, 1998, 2863, 2874, 2961, 3011, 3021, 3117
BseCI ATCGAT 2 cut(s) 730, 3517
BseDI CCNNGG 5 cut(s) 994, 2178, 2862, 3010, 3076
BseJI GATNNNNATC 3 cut(s) 1815, 2841, 2968
BseLI CCNNNNNNNGG 8 cut(s) 1042, 2592, 2868, 2879, 3103, 3208, 3286, 3486
BseMI GCAATG 4 cut(s) 105, 341, 459, 1488
BseMII CTCAG 3 cut(s) 1362, 1538, 2582
BseNI ACTGG 9 cut(s) 653, 792, 1195, 1240, 1415, 2239, 2382, 2526, 2549
BseRI GAGGAG 2 cut(s) 486, 768
BseSI GKGCMC 4 cut(s) 1084, 2445, 3001, 3688
BsgI GTGCAG 2 cut(s) 1701, 2232
BshNI GGYRCC 4 cut(s) 508, 718, 1126, 1232
BshTI ACCGGT 1 cut(s) 83
BshVI ATCGAT 2 cut(s) 730, 3517
BsiHKAI GWGCWC 5 cut(s) 1084, 2536, 3144, 3439, 3688
BsiSI CCGG 6 cut(s) 84, 1783, 2763, 3454, 3803, 3944
BslFI GGGAC 2 cut(s) 717, 1849
BslI CCNNNNNNNGG 8 cut(s) 1042, 2592, 2868, 2879, 3103, 3208, 3286, 3486
BsmAI GTCTC 7 cut(s) 564, 767, 1628, 2115, 2132, 3509, 3759
BsmBI CGTCTC 1 cut(s) 2115
BsmFI GGGAC 2 cut(s) 717, 1849
BsmI GAATGC 4 cut(s) 355, 1874, 2188, 3577
Bso31I GGTCTC 2 cut(s) 564, 3509
Bsp120I GGGCCC 1 cut(s) 2997
Bsp1286I GDGCHC 7 cut(s) 1084, 2445, 2536, 3001, 3144, 3439, 3688
Bsp13I TCCGGA 1 cut(s) 3943
Bsp1407I TGTACA 1 cut(s) 501
BspACI CCGC 8 cut(s) 839, 1248, 1638, 1766, 2052, 3703, 3800, 3908
BspCNI CTCAG 3 cut(s) 1361, 1537, 2583
BspDI ATCGAT 2 cut(s) 730, 3517
BspEI TCCGGA 1 cut(s) 3943
BspHI TCATGA 3 cut(s) 111, 781, 2151
BspMAI CTGCAG 4 cut(s) 550, 823, 3682, 3887
BspMI ACCTGC 1 cut(s) 3671
BspQI GCTCTTC 3 cut(s) 753, 1669, 3597
BspT107I GGYRCC 4 cut(s) 508, 718, 1126, 1232
BspTI CTTAAG 1 cut(s) 698
BspTNI GGTCTC 2 cut(s) 564, 3509
BsrDI GCAATG 4 cut(s) 105, 341, 459, 1488
BsrFI RCCGGY 3 cut(s) 83, 1782, 3453
BsrGI TGTACA 1 cut(s) 501
BsrI ACTGG 9 cut(s) 653, 792, 1195, 1240, 1415, 2239, 2382, 2526, 2549
BssAI RCCGGY 3 cut(s) 83, 1782, 3453
BssECI CCNNGG 5 cut(s) 994, 2178, 2862, 3010, 3076
BssNAI GTATAC 1 cut(s) 61
BssNI GRCGYC 1 cut(s) 3928
BssT1I CCWWGG 2 cut(s) 2178, 3076
Bst1107I GTATAC 1 cut(s) 61
Bst2UI CCWGG 8 cut(s) 996, 1998, 2863, 2874, 2961, 3011, 3021, 3117
Bst6I CTCTTC 7 cut(s) 753, 1334, 1465, 1510, 1669, 2319, 3597
BstACI GRCGYC 1 cut(s) 3928
BstAFI CTTAAG 1 cut(s) 698
BstAPI GCANNNNNTGC 5 cut(s) 352, 1232, 2213, 2791, 3677
BstAUI TGTACA 1 cut(s) 501
BstBAI YACGTR 1 cut(s) 449
BstEII GGTNACC 1 cut(s) 3197
BstENI CCTNNNNNAGG 2 cut(s) 2877, 3101
BstHHI GCGC 2 cut(s) 1782, 3420
BstMAI GTCTC 7 cut(s) 564, 767, 1628, 2115, 2132, 3509, 3759
BstNI CCWGG 8 cut(s) 996, 1998, 2863, 2874, 2961, 3011, 3021, 3117
BstNSI RCATGY 2 cut(s) 1121, 1698
BstPI GGTNACC 1 cut(s) 3197
BstSCI CCNGG 9 cut(s) 994, 1996, 2861, 2872, 2959, 3009, 3019, 3115, 3802
BstSFI CTRYAG 6 cut(s) 546, 819, 1860, 2718, 3678, 3883
BstSLI GKGCMC 4 cut(s) 1084, 2445, 3001, 3688
BstV2I GAAGAC 4 cut(s) 2412, 3048, 3078, 3663
BstX2I RGATCY 8 cut(s) 165, 550, 649, 1598, 2122, 3241, 3406, 3614
BstXI CCANNNNNNTGG 1 cut(s) 2179
BstYI RGATCY 8 cut(s) 165, 550, 649, 1598, 2122, 3241, 3406, 3614
BstZ17I GTATAC 1 cut(s) 61
Bsu15I ATCGAT 2 cut(s) 730, 3517
BsuTUI ATCGAT 2 cut(s) 730, 3517
BtsI GCAGTG 5 cut(s) 543, 1287, 2445, 2976, 3439
BveI ACCTGC 1 cut(s) 3671
CaiI CAGNNNCTG 4 cut(s) 815, 1410, 1582, 2791
CciI TCATGA 3 cut(s) 111, 781, 2151
CfoI GCGC 2 cut(s) 1782, 3420
Cfr10I RCCGGY 3 cut(s) 83, 1782, 3453
Cfr13I GGNCC 8 cut(s) 1151, 1528, 1612, 2522, 2603, 2870, 2997, 2998
ClaI ATCGAT 2 cut(s) 730, 3517
CseI GACGC 1 cut(s) 3936
CsiI ACCWGGT 1 cut(s) 3019
Csp6I GTAC 8 cut(s) 492, 502, 521, 719, 1068, 1127, 1895, 2948
CspAI ACCGGT 1 cut(s) 83
CviQI GTAC 8 cut(s) 492, 502, 521, 719, 1068, 1127, 1895, 2948
DraI TTTAAA 1 cut(s) 688
DraIII CACNNNGTG 2 cut(s) 260, 3023
DrdI GACNNNNNNGTC 1 cut(s) 2740
DriI GACNNNNNGTC 1 cut(s) 2297
DseDI GACNNNNNNGTC 1 cut(s) 2740
EaeI YGGCCR 2 cut(s) 740, 2943
Eam1104I CTCTTC 7 cut(s) 753, 1334, 1465, 1510, 1669, 2319, 3597
Eam1105I GACNNNNNGTC 1 cut(s) 2297
EarI CTCTTC 7 cut(s) 753, 1334, 1465, 1510, 1669, 2319, 3597
EciI GGCGGA 2 cut(s) 2067, 3692
Eco130I CCWWGG 2 cut(s) 2178, 3076
Eco147I AGGCCT 1 cut(s) 3169
Eco24I GRGCYC 1 cut(s) 3001
Eco31I GGTCTC 2 cut(s) 564, 3509
Eco32I GATATC 2 cut(s) 139, 2832
Eco47I GGWCC 3 cut(s) 1151, 1612, 2603
Eco57I CTGAAG 7 cut(s) 321, 888, 1338, 2343, 2501, 2967, 3782
Eco72I CACGTG 1 cut(s) 449
Eco91I GGTNACC 1 cut(s) 3197
EcoNI CCTNNNNNAGG 2 cut(s) 2877, 3101
EcoO109I RGGNCCY 4 cut(s) 1528, 2603, 2997, 2998
EcoO65I GGTNACC 1 cut(s) 3197
EcoRII CCWGG 8 cut(s) 994, 1996, 2861, 2872, 2959, 3009, 3019, 3115
EcoRV GATATC 2 cut(s) 139, 2832
EcoT14I CCWWGG 2 cut(s) 2178, 3076
EcoT22I ATGCAT 3 cut(s) 1465, 1726, 2844
EcoT38I GRGCYC 1 cut(s) 3001
ErhI CCWWGG 2 cut(s) 2178, 3076
Esp3I CGTCTC 1 cut(s) 2115
FalI AAGNNNNNCTT 4 cut(s) 2694, 2726, 3060, 3092
FaqI GGGAC 2 cut(s) 717, 1849
FauI CCCGC 2 cut(s) 1773, 3807
FbaI TGATCA 1 cut(s) 2698
FblI GTMKAC 1 cut(s) 60
FriOI GRGCYC 1 cut(s) 3001
FspBI CTAG 5 cut(s) 518, 795, 2651, 3440, 3482
GlaI GCGC 2 cut(s) 1781, 3419
GsuI CTGGAG 2 cut(s) 809, 2600
HapII CCGG 6 cut(s) 84, 1783, 2763, 3454, 3803, 3944
HgaI GACGC 1 cut(s) 3936
HhaI GCGC 2 cut(s) 1782, 3420
Hin1I GRCGYC 1 cut(s) 3928
Hin6I GCGC 2 cut(s) 1780, 3418
HinP1I GCGC 2 cut(s) 1780, 3418
HincII GTYRAC 2 cut(s) 1369, 2686
HindII GTYRAC 2 cut(s) 1369, 2686
HindIII AAGCTT 3 cut(s) 1935, 2483, 2849
HpaII CCGG 6 cut(s) 84, 1783, 2763, 3454, 3803, 3944
HpyCH4IV ACGT 1 cut(s) 448
HpySE526I ACGT 1 cut(s) 448
Hsp92I GRCGYC 1 cut(s) 3928
HspAI GCGC 2 cut(s) 1780, 3418
Kpn2I TCCGGA 1 cut(s) 3943
KpnI GGTACC 2 cut(s) 722, 1130
KroI GCCGGC 2 cut(s) 1782, 3453
KroNI GCCGGC 2 cut(s) 1784, 3455
Ksp22I TGATCA 1 cut(s) 2698
LguI GCTCTTC 3 cut(s) 753, 1669, 3597
LmnI GCTCC 5 cut(s) 1148, 2768, 3362, 3467, 3743
MabI ACCWGGT 1 cut(s) 3019
MaeI CTAG 5 cut(s) 518, 795, 2651, 3440, 3482
MaeII ACGT 1 cut(s) 448
MaeIII GTNAC 9 cut(s) 128, 241, 377, 1519, 1633, 1864, 2032, 3197, 3828
MfeI CAATTG 1 cut(s) 3954
MflI RGATCY 8 cut(s) 165, 550, 649, 1598, 2122, 3241, 3406, 3614
MhlI GDGCHC 7 cut(s) 1084, 2445, 2536, 3001, 3144, 3439, 3688
MlsI TGGCCA 1 cut(s) 742
MluNI TGGCCA 1 cut(s) 742
MlyI GAGTC 9 cut(s) 15, 685, 769, 1493, 1527, 2426, 2591, 2733, 3836
MmeI TCCRAC 3 cut(s) 384, 3219, 3735
Mox20I TGGCCA 1 cut(s) 742
Mph1103I ATGCAT 3 cut(s) 1465, 1726, 2844
MroI TCCGGA 1 cut(s) 3943
MroNI GCCGGC 2 cut(s) 1782, 3453
MroXI GAANNNNTTC 4 cut(s) 309, 1210, 1343, 2242
MscI TGGCCA 1 cut(s) 742
MslI CAYNNNNRTG 3 cut(s) 200, 1298, 2150
Msp20I TGGCCA 1 cut(s) 742
MspA1I CMGCKG 5 cut(s) 331, 1229, 1248, 2623, 3910
MspCI CTTAAG 1 cut(s) 698
MspI CCGG 6 cut(s) 84, 1783, 2763, 3454, 3803, 3944
MspR9I CCNGG 9 cut(s) 996, 1998, 2863, 2874, 2961, 3011, 3021, 3117, 3804
MunI CAATTG 1 cut(s) 3954
Mva1269I GAATGC 4 cut(s) 355, 1874, 2188, 3577
MvaI CCWGG 8 cut(s) 996, 1998, 2863, 2874, 2961, 3011, 3021, 3117
NaeI GCCGGC 2 cut(s) 1784, 3455
NciI CCSGG 1 cut(s) 3804
NgoMIV GCCGGC 2 cut(s) 1782, 3453
NmuCI GTSAC 5 cut(s) 128, 241, 377, 1519, 3828
NsiI ATGCAT 3 cut(s) 1465, 1726, 2844
NspI RCATGY 2 cut(s) 1121, 1698
PagI TCATGA 3 cut(s) 111, 781, 2151
PaqCI CACCTGC 1 cut(s) 3671
PceI AGGCCT 1 cut(s) 3169
PciI ACATGT 1 cut(s) 1117
PciSI GCTCTTC 3 cut(s) 753, 1669, 3597
PctI GAATGC 4 cut(s) 355, 1874, 2188, 3577
PdiI GCCGGC 2 cut(s) 1784, 3455
PdmI GAANNNNTTC 4 cut(s) 309, 1210, 1343, 2242
PflMI CCANNNNNTGG 1 cut(s) 1042
PfoI TCCNGGA 1 cut(s) 2959
PinAI ACCGGT 1 cut(s) 83
PleI GAGTC 9 cut(s) 14, 684, 769, 1492, 1526, 2425, 2590, 2732, 3835
PmaCI CACGTG 1 cut(s) 449
PmlI CACGTG 1 cut(s) 449
PpsI GAGTC 9 cut(s) 14, 684, 769, 1492, 1526, 2425, 2590, 2732, 3835
Ppu21I YACGTR 1 cut(s) 449
PpuMI RGGWCCY 1 cut(s) 2603
PscI ACATGT 1 cut(s) 1117
PshBI ATTAAT 2 cut(s) 1185, 3357
Psp5II RGGWCCY 1 cut(s) 2603
Psp6I CCWGG 8 cut(s) 994, 1996, 2861, 2872, 2959, 3009, 3019, 3115
PspCI CACGTG 1 cut(s) 449
PspEI GGTNACC 1 cut(s) 3197
PspGI CCWGG 8 cut(s) 994, 1996, 2861, 2872, 2959, 3009, 3019, 3115
PspOMI GGGCCC 1 cut(s) 2997
PspPI GGNCC 8 cut(s) 1151, 1528, 1612, 2522, 2603, 2870, 2997, 2998
PspPPI RGGWCCY 1 cut(s) 2603
PsrI GAACNNNNNNTAC 2 cut(s) 2940, 2972
PstI CTGCAG 4 cut(s) 550, 823, 3682, 3887
PstNI CAGNNNCTG 4 cut(s) 815, 1410, 1582, 2791
PsuI RGATCY 8 cut(s) 165, 550, 649, 1598, 2122, 3241, 3406, 3614
PvuII CAGCTG 3 cut(s) 331, 1229, 2623
RsaI GTAC 8 cut(s) 493, 503, 522, 720, 1069, 1128, 1896, 2949
RsaNI GTAC 8 cut(s) 492, 502, 521, 719, 1068, 1127, 1895, 2948
RseI CAYNNNNRTG 3 cut(s) 200, 1298, 2150
SapI GCTCTTC 3 cut(s) 753, 1669, 3597
Sau96I GGNCC 8 cut(s) 1151, 1528, 1612, 2522, 2603, 2870, 2997, 2998
SchI GAGTC 9 cut(s) 15, 685, 769, 1493, 1527, 2426, 2591, 2733, 3836
ScrFI CCNGG 9 cut(s) 996, 1998, 2863, 2874, 2961, 3011, 3021, 3117, 3804
SduI GDGCHC 7 cut(s) 1084, 2445, 2536, 3001, 3144, 3439, 3688
SexAI ACCWGGT 1 cut(s) 3019
SfcI CTRYAG 6 cut(s) 546, 819, 1860, 2718, 3678, 3883
SinI GGWCC 3 cut(s) 1151, 1612, 2603
SmiMI CAYNNNNRTG 3 cut(s) 200, 1298, 2150
SmlI CTYRAG 4 cut(s) 278, 698, 942, 2846
SmoI CTYRAG 4 cut(s) 278, 698, 942, 2846
SpeI ACTAGT 1 cut(s) 517
SseBI AGGCCT 1 cut(s) 3169
SsiI CCGC 8 cut(s) 839, 1248, 1638, 1766, 2052, 3703, 3800, 3908
SspI AATATT 1 cut(s) 573
SspMI CTAG 5 cut(s) 518, 795, 2651, 3440, 3482
StuI AGGCCT 1 cut(s) 3169
StyD4I CCNGG 9 cut(s) 994, 1996, 2861, 2872, 2959, 3009, 3019, 3115, 3802
StyI CCWWGG 2 cut(s) 2178, 3076
TaiI ACGT 1 cut(s) 451
TaqI TCGA 5 cut(s) 9, 730, 2290, 3267, 3517
TatI WGTACW 2 cut(s) 501, 1894
TauI GCSGC 1 cut(s) 842
TseFI GTSAC 5 cut(s) 128, 241, 377, 1519, 3828
Tsp45I GTSAC 5 cut(s) 128, 241, 377, 1519, 3828
TspGWI ACGGA 3 cut(s) 3553, 3559, 3887
Van91I CCANNNNNTGG 1 cut(s) 1042
Vha464I CTTAAG 1 cut(s) 698
VneI GTGCAC 2 cut(s) 1080, 3684
VpaK11BI GGWCC 3 cut(s) 1151, 1612, 2603
VspI ATTAAT 2 cut(s) 1185, 3357
XagI CCTNNNNNAGG 2 cut(s) 2877, 3101
XceI RCATGY 2 cut(s) 1121, 1698
XcmI CCANNNNNNNNNTGG 1 cut(s) 2374
XmiI GTMKAC 1 cut(s) 60
XmnI GAANNNNTTC 4 cut(s) 309, 1210, 1343, 2242
XspI CTAG 5 cut(s) 518, 795, 2651, 3440, 3482
Zsp2I ATGCAT 3 cut(s) 1465, 1726, 2844
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.