Prupe.2G324200_v2.0.a1

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0004502
Physical Location & Seq
Forward (+)
29994750 .. 30003989
9240 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G324200.1

Sequence Viewer

Length: 4047 bp
ATGGGTGTGGAGAACTACCATGTGATTGAGCTTGTAGGCGAAGGTTCCTTTGGGAAGGTATACAAGGGAAGGCGCAAGTACACCGGGCAGACTGTTGCAATGAAATTCATCATGAAACATGGGAAAAGCGACAAAGATATTCACAATTTAAGACAAGAAATTGAGATCCTAAGAAAGTTGAAGCATGAAAATATAATTGAAATGCTTGATTCCTTTGAAAGCCCACAAGAGTTCTGTGTTGTCACAGAATTTGCACAAGGTGAGCTATTTGAAATTCTTGAGGATGATAAGTGCCTTCCCGAAGAACAAGTTCAAGCAATTGCAAAGCAGTTGGTGAGAGCGTTGCATTATCTGCATTCCAACCGTATCATTCATCGTGACATGAAACCACAAAACATTCTAATTGGTGCTGGTTCTATTGTCAAGCTCTGTGATTTTGGGTTTGCACGCGCAATGTCCACAAATACTGTTGTTTTGCGATCCATTAAAGGTACTCCTCTGTACATGGCACCTGAACTAGTACGGGAACAACCCTACAATCACACTGCGGATTTATGGTCTCTTGGAGTAATATTATATGAATTATTTGTTGGCCAGCCTCCATTTTATACAAATTCTGTATATGCACTCATCAGGCACATTGTTAAGGATCCGGTTAAATACCCTGACAACATGAGTCCAAGTTTTAAAAGCTTTCTGAAGGGATTGCTCAATAAGGTACCTCAAAATCGATTGACTTGGCCAGCTCTTCTGGAGCACCCATTTGTTAAAGAGATGCCTCATGAAGTGGAGGCTAGGGAAATGCGTTCTGCAACTGCTGCAGAAAGAGGATGTGTTGCGGCATGGAGGGGTGAAGGAAACACAGTTCAAACATCAGTTGGTTTGGCTGTTTCTTCTCCTGTCAACTCTCCAGATTCTTCAGAAAACAACAGTGGAATAAGTTTTCAGGGTGATGCTCAATCAGACATTCCTGACTGCACAGCAGTCAATTCTTCCCCAAATGAGTTCCCAGGATTTGCAAATCCTAATGAAGTTAAACAGTCAGGTTGCCAAATTTTGGACAGATTGGAAAATAACTCTCGTACAGTTAAAGGTGCACAAATAATTAGTCAAGATAATGAAGCAGTAGCACATGTTTTGCTACCACTTAAAAGATGTTCCCAAGGATCCCCAAATTCGTGCAGAGATCAAGATATTCTTAATTCAAACCAGTCTTTGAGGATCCTTTCAAACTTAGTTGCGGCTGGTGCCATCCATTCCAGTGGGCTACTTGATGAAATAATACATGAACTTCTTGTTTACACTGGCATCATAGTTAGCATGAAAGCTTCTGAAGTGAATGAGTTAAAAGCAAAGAGTTTCTCAATAATTAAAATTTTGGTAGACAATGCCGGAAGTGGCGCTGGAGGCTCATATTTCAGGCACTGGGTTACGTTTGCTGATATTTTTTCGCAAGTTGTTGGTTGCAGCGAAGATGCATCCGGACGAGTTCTGTATGAGTCCATTGCTTGCATAACTGTTGTGTTAACCAGAGTCACTCAGGGCCTTAAAGCAGTTTCTTCAACTTCAGTTCCTGAGGCGGTTTCTGATCCTAATGAAACATGGAAACAGATTTTGGATCACGCTAAAACATCTGGTTTAGTGGATCAACTTTGTCTCTGCTTAGTTACTGCGGGGTCAAGTCTTATTTCAGGTTCTTCAAATATGCTGCGTGCAGCTTGTGAAGCATGTAGGGCTATTTGGTTGCTGGTAGATGCATCAGAAAATCTTTCTATGAAAAGAAATGCCTATTCATTTCCACTTAACACTATGCGCAGTCCTTCACTCCAACTTGGCATTAGGGATCAAGATCAAAGTTCTTTAATTGGGACAGAATCAGCAAAACTTGTTGCTGCAGTGACTAGGGCATTCCTTAGATCAAAAGCAGTACAGGTTGCTATACATTATTGCCTTCATCAACGACTTGAGGCTTCACTGTATGCTAGCATTCAGCTACTGTTGAGGTGCTGCTTGCATAATGGAATTGTTCCTGGTATGCTCTGTGGCCTGCCTAGTTCCCTACCTGTAACTACTGTTGTCAGTGGTGGGGGTGATGGAACCATTATTTCAGAGATATTCTCGTTGTTGTCTTTGTGCATTTCATCTCAAAATAGAGATCCACAAGCAATTGAAACAACCAACTTGAAGTGCAAATTAACTAATCCCACTACCTTGGTTCTGCATTCGTGCCTCATCCTTGCAACAATTGCACAATGTTTGAAGGCAACTGGAAGAAATTCTGCATTATTTATGTTGACAACCTCCCCAAAGAAGCAGCTTTCTCGACTTTCTGTCCTCGCTCATCATTTTTCTTCTGATGAGAGCACAAACACTTCCTTTCAAACTCATACAGCATCGGCCATGTTGGCACTGGCTTCCATATTGTCGCTTGAATCTGGAGCTTCTGTTGGGTCCTCTGTCTCCGAAGTAGCAGTGCCTTTGATTCCTCGAAGTGCCACACTATGTGACTACCTCAAAGTTTCACCAGGCAGTGGAATTGAATTGGGTCCTAATGGCACCAAGAGTGCTCTCTCATACTGGCATGGTCTTAGGGATGGATGTGTTGGCTTGCTGGAGTCCAGACTGAGGTGGGGAGGACCCTTAGTTGTCAAACAGCTGTGTACGAGCAATATCCCTCTTCTTCTAGTCAGTTTGTTAGCCAAAAACCAACAAAATGTGTCTCCACAAGAAGTTGACAGCACAAATGATCAAGTTGGGCTTTCCCCTATCGGAGTTGTGTGGACAATATCGTCAATATGTCACTGCCTCTCAGGTGGAGCCTTAACTTTTCGTCAGATTTTGCTCAGAAGTGATCACATCAAGCTCATCTCTGACCTGATATCTGATATGCATCTCAAGCTCGTAAAGTCCTGGGTTGGGCCTGGTGGAGGGAAGGATGGTGTGAGAGATATAATAAATGCAGTAATTGATCTCTTGGCATTTCCTTTTGTGGCCGTACAGAATGCTCCGGGATTGCTATCAGCCACTGCTTCAGTGAACAGCGGGGCCCTTCTCAACATGGGTTCACCAGGTGTGAGAGTTGGCATGGAAGACAGAGACATGGTGAAAGTAATTGAGGAAGACCTGGGAAAGTATATTAAAAACCTTTTGGAGGTGGGAGTGCCAGGCATCATTCTTCGATGTTTAGATAATTTGGAGTTAAAAGATATAGGAAGGCCTGTGGCCTTTCTTGCTAAGATGATTGGTCACCGACCATTGGCAGTTCAACTTGTGGGTAAAGGCTTGTTGGATCCCACTAGGATGAGAAGATTGCTCGATTGTTCAAGCCCAAGGGAGGTGATGCTGGATGTTCTGATGATTGTTTCTGATTTGGCTCGCATGGACAAGGGATTCTACGAATACATTAACGGGGCTTCTGTACTGGAGTTCTTTAAGGAATTTCTTACCCATGAGGATCCTAATGTGCGCTCAAAGACTTGCAGTGCTCTAGGCAATATGTGCCGGCACAGCTCCTACTTTTATAGTGCCCTAGCAAAGCATCAAATCATTGGCCTCCTTATTGATCGATGCTCTGATCCAGACAAACGGACACGAAAATTTGCTTGCTTTGCTATCGGAAATGCTGCCTACCATAATGACATGTTATATGATGAGCTACGAAGATCTATACCCCATCTTGCCAATTTGCTGCTTTCAACCGAAGAAGACAAGACAAAAGCAAATGCTGCCGGTGCACTAAGCAATCTTGTCCGCAACTCCAACAAACTTTGTGAAGACATTGTATCTAAAGGAGCGATGCAGTCTTTACTGAAGTTGGTGGCTGACTGTTCGGTGGTAGCACTGAACCCAGGTAGGAAAGATTCAGTAAATGAGTCACCTCTAAAAATAGCTCTCTTTTCATTGGCAAAGATGTGTTCGCATCCGCCGTGCAAACAATTCCTCCGTTCATCAGAGTTGTTCTCAGTGATCGGACGGCTTCGGCAATCTCCAGAATCTACAATTGCCAATTATGCCTCCGTCATCATCACCAAAGTTGCTGATTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000166 GO:0000278 GO:0000280 GO:0000281 GO:0000910 GO:0000911 GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005856 GO:0005874 GO:0005881 GO:0006355 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006935 GO:0006996 GO:0007049 GO:0007112 GO:0007140 GO:0007154 GO:0007163 GO:0007165 GO:0007166 GO:0007224 GO:0007227 GO:0007228 GO:0007275 GO:0007276 GO:0007349 GO:0007584 GO:0008064 GO:0008092 GO:0008134 GO:0008144 GO:0008150 GO:0008152 GO:0008589 GO:0009524 GO:0009553 GO:0009558 GO:0009561 GO:0009605 GO:0009653 GO:0009791 GO:0009889 GO:0009966 GO:0009967 GO:0009987 GO:0009991 GO:0010033 GO:0010243 GO:0010468 GO:0010556 GO:0010639 GO:0010646 GO:0010647 GO:0014070 GO:0015630 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017076 GO:0019219 GO:0019222 GO:0019538 GO:0019894 GO:0019953 GO:0022402 GO:0022412 GO:0022414 GO:0022607 GO:0023051 GO:0023052 GO:0023056 GO:0030010 GO:0030030 GO:0030031 GO:0030554 GO:0030832 GO:0030833 GO:0030837 GO:0031156 GO:0031272 GO:0031323 GO:0031326 GO:0031333 GO:0031344 GO:0031667 GO:0032101 GO:0032104 GO:0032107 GO:0032271 GO:0032272 GO:0032501 GO:0032502 GO:0032504 GO:0032506 GO:0032535 GO:0032553 GO:0032555 GO:0032559 GO:0032956 GO:0032970 GO:0033043 GO:0033206 GO:0033273 GO:0035556 GO:0035639 GO:0036094 GO:0036211 GO:0040011 GO:0042221 GO:0042330 GO:0042493 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043254 GO:0043326 GO:0043327 GO:0043412 GO:0043900 GO:0044085 GO:0044087 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0044703 GO:0044782 GO:0045880 GO:0048229 GO:0048232 GO:0048285 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048609 GO:0048646 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051090 GO:0051091 GO:0051128 GO:0051129 GO:0051171 GO:0051252 GO:0051301 GO:0051321 GO:0051493 GO:0051494 GO:0051593 GO:0051704 GO:0051716 GO:0060176 GO:0060255 GO:0060271 GO:0060491 GO:0061640 GO:0065007 GO:0065008 GO:0065009 GO:0070925 GO:0071704 GO:0071840 GO:0080090 GO:0080134 GO:0080135 GO:0090066 GO:0097159 GO:0097367 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0110053 GO:0120031 GO:0120032 GO:0120035 GO:0120036 GO:0140013 GO:0140096 GO:1901265 GO:1901363 GO:1901564 GO:1901698 GO:1901700 GO:1902410 GO:1902903 GO:1902904 GO:1903046 GO:1903047 GO:1903506 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04341 map04341
Pfam Domains
Protein Families
Arm HEAT HEAT_2 Pkinase

Protein Analysis

1349

Amino Acids

146.71

Weight (kDa)

7.91

Isoelectric Point (pI)

47.1

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2788
Acc16I TGCGCA 1 cut(s) 1814
Acc65I GGTACC 1 cut(s) 718
AccB1I GGYRCC 4 cut(s) 508, 718, 1247, 2555
AccB7I CCANNNNNTGG 2 cut(s) 1057, 2531
AccI GTMKAC 2 cut(s) 60, 1383
AccII CGCG 1 cut(s) 450
AccIII TCCGGA 1 cut(s) 1481
AciI CCGC 8 cut(s) 548, 839, 1241, 1580, 1673, 3042, 3751, 3923
AcoI YGGCCR 4 cut(s) 592, 740, 2397, 2991
AcuI CTGAAG 6 cut(s) 719, 903, 1353, 1551, 3015, 3830
AdeI CACNNNGTG 3 cut(s) 260, 2504, 3071
AfiI CCNNNNNNNGG 8 cut(s) 1057, 2531, 2625, 2916, 2927, 3151, 3256, 3334
AflIII ACRYGT 2 cut(s) 1132, 3639
AhdI GACNNNNNGTC 1 cut(s) 2330
AhlI ACTAGT 1 cut(s) 517
AjnI CCWGG 9 cut(s) 1009, 2029, 2524, 2909, 2920, 3067, 3123, 3163, 3847
AjuI GAANNNNNNNTTGG 6 cut(s) 33, 65, 573, 605, 1598, 1630
AloI GAACNNNNNNTCC 2 cut(s) 849, 881
Alw21I GWGCWC 6 cut(s) 759, 1099, 2366, 2569, 3487, 3736
Alw26I GTCTC 5 cut(s) 564, 1661, 2464, 2724, 3090
Alw44I GTGCAC 2 cut(s) 1095, 3732
AlwNI CAGNNNCTG 4 cut(s) 1425, 1574, 1996, 3026
Aor13HI TCCGGA 1 cut(s) 1481
ApaI GGGCCC 1 cut(s) 3049
ApaLI GTGCAC 2 cut(s) 1095, 3732
ArsI GACNNNNNNTTYG 2 cut(s) 1093, 1125
Asp700I GAANNNNTTC 2 cut(s) 309, 2275
Asp718I GGTACC 1 cut(s) 718
AspLEI GCGC 5 cut(s) 75, 452, 1403, 1815, 3468
AspS9I GGNCC 7 cut(s) 1543, 2451, 2546, 2636, 2918, 3045, 3046
AsuC2I CCSGG 2 cut(s) 85, 3009
AsuNHI GCTAGC 1 cut(s) 1982
AvaII GGWCC 3 cut(s) 2451, 2546, 2636
AxyI CCTNAGG 1 cut(s) 1575
BaeGI GKGCMC 4 cut(s) 1099, 3049, 3529, 3736
BalI TGGCCA 2 cut(s) 594, 742
BamHI GGATCC 5 cut(s) 649, 1166, 1221, 3289, 3454
BanI GGYRCC 4 cut(s) 508, 718, 1247, 2555
BanII GRGCYC 1 cut(s) 3049
BbsI GAAGAC 4 cut(s) 3096, 3126, 3711, 3780
Bbv12I GWGCWC 6 cut(s) 759, 1099, 2366, 2569, 3487, 3736
BccI CCATC 5 cut(s) 1259, 2087, 2588, 2930, 3681
BceAI ACGGC 3 cut(s) 2978, 3910, 3989
BcgI CGANNNNNNTGC 2 cut(s) 2303, 2337
BciT130I CCWGG 9 cut(s) 1011, 2031, 2526, 2911, 2922, 3069, 3125, 3165, 3849
BclI TGATCA 2 cut(s) 2746, 2851
BcnI CCSGG 2 cut(s) 85, 3009
BcoDI GTCTC 5 cut(s) 564, 1661, 2464, 2724, 3090
BcuI ACTAGT 1 cut(s) 517
BfaI CTAG 9 cut(s) 518, 795, 1902, 1983, 2052, 2684, 3297, 3488, 3530
BfmI CTRYAG 2 cut(s) 819, 1893
BfoI RGCGCY 1 cut(s) 1404
BglI GCCNNNNNGGC 1 cut(s) 2405
BglII AGATCT 1 cut(s) 3662
Bme18I GGWCC 3 cut(s) 2451, 2546, 2636
BmeRI GACNNNNNGTC 1 cut(s) 2330
BmgT120I GGNCC 7 cut(s) 1543, 2451, 2546, 2636, 2918, 3045, 3046
BmrI ACTGGG 1 cut(s) 1435
BmtI GCTAGC 1 cut(s) 1986
BmuI ACTGGG 1 cut(s) 1435
BpiI GAAGAC 4 cut(s) 3096, 3126, 3711, 3780
BplI GAGNNNNNCTC 4 cut(s) 2102, 2134, 3944, 3976
BpmI CTGGAG 7 cut(s) 773, 894, 1425, 2457, 2633, 3443, 3972
BpuEI CTTGAG 3 cut(s) 299, 1985, 2879
BpuMI CCSGG 2 cut(s) 85, 3009
Bsa29I ATCGAT 2 cut(s) 730, 3565
BsaBI GATNNNNATC 3 cut(s) 1848, 2889, 3016
BsaI GGTCTC 1 cut(s) 564
BsaJI CCNNGG 7 cut(s) 1009, 1162, 2211, 2910, 3124, 3329, 3847
BsaWI WCCGGW 2 cut(s) 652, 1481
BsaXI ACNNNNNCTCC 4 cut(s) 2430, 2460, 3924, 3954
Bsc4I CCNNNNNNNGG 8 cut(s) 1057, 2531, 2625, 2916, 2927, 3151, 3256, 3334
Bse118I RCCGGY 2 cut(s) 3501, 3728
Bse1I ACTGG 8 cut(s) 1210, 1260, 1309, 1430, 2272, 2415, 2582, 3426
Bse21I CCTNAGG 1 cut(s) 1575
Bse3DI GCAATG 3 cut(s) 105, 459, 1503
Bse8I GATNNNNATC 3 cut(s) 1848, 2889, 3016
BseAI TCCGGA 1 cut(s) 1481
BseBI CCWGG 9 cut(s) 1011, 2031, 2526, 2911, 2922, 3069, 3125, 3165, 3849
BseCI ATCGAT 2 cut(s) 730, 3565
BseDI CCNNGG 7 cut(s) 1009, 1162, 2211, 2910, 3124, 3329, 3847
BseJI GATNNNNATC 3 cut(s) 1848, 2889, 3016
BseLI CCNNNNNNNGG 8 cut(s) 1057, 2531, 2625, 2916, 2927, 3151, 3256, 3334
BseMI GCAATG 3 cut(s) 105, 459, 1503
BseMII CTCAG 6 cut(s) 1553, 1566, 2615, 2823, 2857, 3975
BseNI ACTGG 8 cut(s) 1210, 1260, 1309, 1430, 2272, 2415, 2582, 3426
BseRI GAGGAG 1 cut(s) 486
BseSI GKGCMC 4 cut(s) 1099, 3049, 3529, 3736
BsgI GTGCAG 3 cut(s) 961, 1201, 1734
Bsh1236I CGCG 1 cut(s) 450
BshNI GGYRCC 4 cut(s) 508, 718, 1247, 2555
BshVI ATCGAT 2 cut(s) 730, 3565
BsiHKAI GWGCWC 6 cut(s) 759, 1099, 2366, 2569, 3487, 3736
BsiSI CCGG 7 cut(s) 84, 653, 1392, 1482, 3008, 3502, 3729
BslFI GGGAC 1 cut(s) 1882
BslI CCNNNNNNNGG 8 cut(s) 1057, 2531, 2625, 2916, 2927, 3151, 3256, 3334
BsmAI GTCTC 5 cut(s) 564, 1661, 2464, 2724, 3090
BsmFI GGGAC 1 cut(s) 1882
BsmI GAATGC 5 cut(s) 355, 1907, 1986, 2221, 3007
Bso31I GGTCTC 1 cut(s) 564
Bsp120I GGGCCC 1 cut(s) 3045
Bsp1286I GDGCHC 8 cut(s) 759, 1099, 2366, 2569, 3049, 3487, 3529, 3736
Bsp13I TCCGGA 1 cut(s) 1481
Bsp1407I TGTACA 1 cut(s) 501
BspACI CCGC 8 cut(s) 548, 839, 1241, 1580, 1673, 3042, 3751, 3923
BspCNI CTCAG 6 cut(s) 1552, 1567, 2616, 2822, 2856, 3974
BspDI ATCGAT 2 cut(s) 730, 3565
BspEI TCCGGA 1 cut(s) 1481
BspFNI CGCG 1 cut(s) 450
BspHI TCATGA 2 cut(s) 111, 781
BspMAI CTGCAG 2 cut(s) 823, 1897
BspOI GCTAGC 1 cut(s) 1986
BspQI GCTCTTC 1 cut(s) 753
BspT107I GGYRCC 4 cut(s) 508, 718, 1247, 2555
BspTNI GGTCTC 1 cut(s) 564
BsrDI GCAATG 3 cut(s) 105, 459, 1503
BsrFI RCCGGY 2 cut(s) 3501, 3728
BsrGI TGTACA 1 cut(s) 501
BsrI ACTGG 8 cut(s) 1210, 1260, 1309, 1430, 2272, 2415, 2582, 3426
BssAI RCCGGY 2 cut(s) 3501, 3728
BssECI CCNNGG 7 cut(s) 1009, 1162, 2211, 2910, 3124, 3329, 3847
BssNAI GTATAC 1 cut(s) 61
BssT1I CCWWGG 3 cut(s) 1162, 2211, 3329
Bst1107I GTATAC 1 cut(s) 61
Bst2UI CCWGG 9 cut(s) 1011, 2031, 2526, 2911, 2922, 3069, 3125, 3165, 3849
Bst6I CTCTTC 2 cut(s) 753, 2682
BstAPI GCANNNNNTGC 5 cut(s) 352, 818, 2246, 3498, 3725
BstAUI TGTACA 1 cut(s) 501
BstEII GGTNACC 1 cut(s) 3245
BstENI CCTNNNNNAGG 2 cut(s) 2925, 3149
BstFNI CGCG 1 cut(s) 450
BstH2I RGCGCY 1 cut(s) 1404
BstHHI GCGC 5 cut(s) 75, 452, 1403, 1815, 3468
BstMAI GTCTC 5 cut(s) 564, 1661, 2464, 2724, 3090
BstNI CCWGG 9 cut(s) 1011, 2031, 2526, 2911, 2922, 3069, 3125, 3165, 3849
BstNSI RCATGY 3 cut(s) 1136, 1731, 3643
BstPI GGTNACC 1 cut(s) 3245
BstSFI CTRYAG 2 cut(s) 819, 1893
BstSLI GKGCMC 4 cut(s) 1099, 3049, 3529, 3736
BstUI CGCG 1 cut(s) 450
BstV2I GAAGAC 4 cut(s) 3096, 3126, 3711, 3780
BstX2I RGATCY 8 cut(s) 165, 649, 1166, 1221, 2155, 3289, 3454, 3662
BstXI CCANNNNNNTGG 2 cut(s) 1262, 2212
BstYI RGATCY 8 cut(s) 165, 649, 1166, 1221, 2155, 3289, 3454, 3662
BstZ17I GTATAC 1 cut(s) 61
Bsu15I ATCGAT 2 cut(s) 730, 3565
Bsu36I CCTNAGG 1 cut(s) 1575
BsuTUI ATCGAT 2 cut(s) 730, 3565
BtgZI GCGATG 1 cut(s) 3809
BtsI GCAGTG 7 cut(s) 543, 1902, 2478, 2536, 2800, 3024, 3487
CaiI CAGNNNCTG 4 cut(s) 1425, 1574, 1996, 3026
CciI TCATGA 2 cut(s) 111, 781
CfoI GCGC 5 cut(s) 75, 452, 1403, 1815, 3468
Cfr10I RCCGGY 2 cut(s) 3501, 3728
Cfr13I GGNCC 7 cut(s) 1543, 2451, 2546, 2636, 2918, 3045, 3046
ClaI ATCGAT 2 cut(s) 730, 3565
CsiI ACCWGGT 1 cut(s) 3067
DraI TTTAAA 1 cut(s) 688
DraIII CACNNNGTG 3 cut(s) 260, 2504, 3071
DrdI GACNNNNNNGTC 1 cut(s) 2788
DriI GACNNNNNGTC 1 cut(s) 2330
DseDI GACNNNNNNGTC 1 cut(s) 2788
EaeI YGGCCR 4 cut(s) 592, 740, 2397, 2991
Eam1104I CTCTTC 2 cut(s) 753, 2682
Eam1105I GACNNNNNGTC 1 cut(s) 2330
EarI CTCTTC 2 cut(s) 753, 2682
EciI GGCGGA 1 cut(s) 3912
Eco130I CCWWGG 3 cut(s) 1162, 2211, 3329
Eco147I AGGCCT 1 cut(s) 3217
Eco24I GRGCYC 1 cut(s) 3049
Eco31I GGTCTC 1 cut(s) 564
Eco32I GATATC 1 cut(s) 2880
Eco47I GGWCC 3 cut(s) 2451, 2546, 2636
Eco57I CTGAAG 6 cut(s) 719, 903, 1353, 1551, 3015, 3830
Eco81I CCTNAGG 1 cut(s) 1575
Eco91I GGTNACC 1 cut(s) 3245
EcoNI CCTNNNNNAGG 2 cut(s) 2925, 3149
EcoO109I RGGNCCY 6 cut(s) 1543, 2451, 2546, 2636, 3045, 3046
EcoO65I GGTNACC 1 cut(s) 3245
EcoRII CCWGG 9 cut(s) 1009, 2029, 2524, 2909, 2920, 3067, 3123, 3163, 3847
EcoRV GATATC 1 cut(s) 2880
EcoT14I CCWWGG 3 cut(s) 1162, 2211, 3329
EcoT22I ATGCAT 3 cut(s) 1480, 1759, 2892
EcoT38I GRGCYC 1 cut(s) 3049
ErhI CCWWGG 3 cut(s) 1162, 2211, 3329
FalI AAGNNNNNCTT 4 cut(s) 1182, 1214, 2742, 2774
FaqI GGGAC 1 cut(s) 1882
FauI CCCGC 2 cut(s) 1666, 3035
FbaI TGATCA 2 cut(s) 2746, 2851
FblI GTMKAC 2 cut(s) 60, 1383
FriOI GRGCYC 1 cut(s) 3049
FspBI CTAG 9 cut(s) 518, 795, 1902, 1983, 2052, 2684, 3297, 3488, 3530
FspI TGCGCA 1 cut(s) 1814
GlaI GCGC 5 cut(s) 74, 451, 1402, 1814, 3467
GsuI CTGGAG 7 cut(s) 773, 894, 1425, 2457, 2633, 3443, 3972
HaeII RGCGCY 1 cut(s) 1404
HapII CCGG 7 cut(s) 84, 653, 1392, 1482, 3008, 3502, 3729
HhaI GCGC 5 cut(s) 75, 452, 1403, 1815, 3468
Hin6I GCGC 5 cut(s) 73, 450, 1401, 1813, 3466
HinP1I GCGC 5 cut(s) 73, 450, 1401, 1813, 3466
HincII GTYRAC 4 cut(s) 904, 1527, 2295, 2734
HindII GTYRAC 4 cut(s) 904, 1527, 2295, 2734
HindIII AAGCTT 2 cut(s) 691, 1326
HpaI GTTAAC 1 cut(s) 1527
HpaII CCGG 7 cut(s) 84, 653, 1392, 1482, 3008, 3502, 3729
HpyCH4IV ACGT 1 cut(s) 1433
HpySE526I ACGT 1 cut(s) 1433
HspAI GCGC 5 cut(s) 73, 450, 1401, 1813, 3466
Kpn2I TCCGGA 1 cut(s) 1481
KpnI GGTACC 1 cut(s) 722
KroI GCCGGC 1 cut(s) 3501
KroNI GCCGGC 1 cut(s) 3503
Ksp22I TGATCA 2 cut(s) 2746, 2851
KspAI GTTAAC 1 cut(s) 1527
LguI GCTCTTC 1 cut(s) 753
LmnI GCTCC 6 cut(s) 754, 2438, 2816, 3010, 3515, 3791
MabI ACCWGGT 1 cut(s) 3067
MaeI CTAG 9 cut(s) 518, 795, 1902, 1983, 2052, 2684, 3297, 3488, 3530
MaeII ACGT 1 cut(s) 1433
MfeI CAATTG 4 cut(s) 318, 2166, 2244, 3999
MflI RGATCY 8 cut(s) 165, 649, 1166, 1221, 2155, 3289, 3454, 3662
MhlI GDGCHC 8 cut(s) 759, 1099, 2366, 2569, 3049, 3487, 3529, 3736
MlsI TGGCCA 2 cut(s) 594, 742
MluNI TGGCCA 2 cut(s) 594, 742
MlyI GAGTC 5 cut(s) 685, 1508, 1542, 2624, 3881
MmeI TCCRAC 4 cut(s) 384, 1852, 3267, 3783
Mox20I TGGCCA 2 cut(s) 594, 742
Mph1103I ATGCAT 3 cut(s) 1480, 1759, 2892
MroI TCCGGA 1 cut(s) 1481
MroNI GCCGGC 1 cut(s) 3501
MroXI GAANNNNTTC 2 cut(s) 309, 2275
MscI TGGCCA 2 cut(s) 594, 742
MslI CAYNNNNRTG 2 cut(s) 1260, 3299
Msp20I TGGCCA 2 cut(s) 594, 742
MspA1I CMGCKG 2 cut(s) 2656, 3042
MspI CCGG 7 cut(s) 84, 653, 1392, 1482, 3008, 3502, 3729
MunI CAATTG 4 cut(s) 318, 2166, 2244, 3999
Mva1269I GAATGC 5 cut(s) 355, 1907, 1986, 2221, 3007
MvaI CCWGG 9 cut(s) 1011, 2031, 2526, 2911, 2922, 3069, 3125, 3165, 3849
MvnI CGCG 1 cut(s) 450
NaeI GCCGGC 1 cut(s) 3503
NciI CCSGG 2 cut(s) 85, 3009
NgoMIV GCCGGC 1 cut(s) 3501
NheI GCTAGC 1 cut(s) 1982
NmuCI GTSAC 8 cut(s) 241, 377, 1534, 1897, 2504, 2798, 3245, 3873
NsbI TGCGCA 1 cut(s) 1814
NsiI ATGCAT 3 cut(s) 1480, 1759, 2892
NspI RCATGY 3 cut(s) 1136, 1731, 3643
PagI TCATGA 2 cut(s) 111, 781
PceI AGGCCT 1 cut(s) 3217
PciI ACATGT 2 cut(s) 1132, 3639
PciSI GCTCTTC 1 cut(s) 753
PcsI WCGNNNNNNNCGW 1 cut(s) 3923
PctI GAATGC 5 cut(s) 355, 1907, 1986, 2221, 3007
PdiI GCCGGC 1 cut(s) 3503
PdmI GAANNNNTTC 2 cut(s) 309, 2275
PfeI GAWTC 9 cut(s) 209, 914, 1874, 2432, 2482, 3390, 3860, 3992, 4040
PflMI CCANNNNNTGG 2 cut(s) 1057, 2531
PfoI TCCNGGA 1 cut(s) 3007
PleI GAGTC 5 cut(s) 684, 1507, 1541, 2623, 3880
PpsI GAGTC 5 cut(s) 684, 1507, 1541, 2623, 3880
PpuMI RGGWCCY 3 cut(s) 2451, 2546, 2636
PscI ACATGT 2 cut(s) 1132, 3639
Psp5II RGGWCCY 3 cut(s) 2451, 2546, 2636
Psp6I CCWGG 9 cut(s) 1009, 2029, 2524, 2909, 2920, 3067, 3123, 3163, 3847
PspEI GGTNACC 1 cut(s) 3245
PspGI CCWGG 9 cut(s) 1009, 2029, 2524, 2909, 2920, 3067, 3123, 3163, 3847
PspOMI GGGCCC 1 cut(s) 3045
PspPI GGNCC 7 cut(s) 1543, 2451, 2546, 2636, 2918, 3045, 3046
PspPPI RGGWCCY 3 cut(s) 2451, 2546, 2636
PstI CTGCAG 2 cut(s) 823, 1897
PstNI CAGNNNCTG 4 cut(s) 1425, 1574, 1996, 3026
PsuI RGATCY 8 cut(s) 165, 649, 1166, 1221, 2155, 3289, 3454, 3662
PvuII CAGCTG 1 cut(s) 2656
RseI CAYNNNNRTG 2 cut(s) 1260, 3299
SapI GCTCTTC 1 cut(s) 753
Sau96I GGNCC 7 cut(s) 1543, 2451, 2546, 2636, 2918, 3045, 3046
SchI GAGTC 5 cut(s) 685, 1508, 1542, 2624, 3881
SduI GDGCHC 8 cut(s) 759, 1099, 2366, 2569, 3049, 3487, 3529, 3736
SexAI ACCWGGT 1 cut(s) 3067
SfcI CTRYAG 2 cut(s) 819, 1893
SinI GGWCC 3 cut(s) 2451, 2546, 2636
SmiMI CAYNNNNRTG 2 cut(s) 1260, 3299
SmlI CTYRAG 3 cut(s) 278, 1964, 2894
SmoI CTYRAG 3 cut(s) 278, 1964, 2894
SpeI ACTAGT 1 cut(s) 517
SseBI AGGCCT 1 cut(s) 3217
SsiI CCGC 8 cut(s) 548, 839, 1241, 1580, 1673, 3042, 3751, 3923
SspI AATATT 1 cut(s) 573
SspMI CTAG 9 cut(s) 518, 795, 1902, 1983, 2052, 2684, 3297, 3488, 3530
StuI AGGCCT 1 cut(s) 3217
StyI CCWWGG 3 cut(s) 1162, 2211, 3329
TaiI ACGT 1 cut(s) 1436
TaqI TCGA 6 cut(s) 730, 2323, 2488, 3178, 3315, 3565
TatI WGTACW 4 cut(s) 78, 501, 1927, 3418
TauI GCSGC 2 cut(s) 842, 1244
TfiI GAWTC 9 cut(s) 209, 914, 1874, 2432, 2482, 3390, 3860, 3992, 4040
TseFI GTSAC 8 cut(s) 241, 377, 1534, 1897, 2504, 2798, 3245, 3873
Tsp45I GTSAC 8 cut(s) 241, 377, 1534, 1897, 2504, 2798, 3245, 3873
TspGWI ACGGA 3 cut(s) 3601, 3932, 4006
Van91I CCANNNNNTGG 2 cut(s) 1057, 2531
VneI GTGCAC 2 cut(s) 1095, 3732
VpaK11BI GGWCC 3 cut(s) 2451, 2546, 2636
XagI CCTNNNNNAGG 2 cut(s) 2925, 3149
XceI RCATGY 3 cut(s) 1136, 1731, 3643
XcmI CCANNNNNNNNNTGG 1 cut(s) 2407
XmiI GTMKAC 2 cut(s) 60, 1383
XmnI GAANNNNTTC 2 cut(s) 309, 2275
XspI CTAG 9 cut(s) 518, 795, 1902, 1983, 2052, 2684, 3297, 3488, 3530
Zsp2I ATGCAT 3 cut(s) 1480, 1759, 2892
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.