AT1G53035

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0003774
Physical Location & Seq
Reverse (-)
19761550 .. 19762561
1012 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G53035.2

Sequence Viewer

Length: 453 bp
ATGACTACAGCAATATCGATGAATCCATCTTTGTTTCGAGTAATCTGTATACTCCATTCGATAATTGCGCTTACTAGTGGAACCTTAATGATGTTCTACACAGAGAAAGCTTCAATCTTTGGACCAGGAAGTGAGATTGCTAGCAAACTAAAAGGATCAACACCACACGATGAACTACTCATACAGATTTCTCAGTCATTCTCTGGTTTGCTTCTGTTTGCAATTGGTTTGGTACTGTTCATGGTTTCGTTTGTGAAAGACAAAGAGTTTCATAGCTTCTTCGCTGGTGGGTCCGTGATTCTGTATGTGTTAATGGCTATGTGGAGGGTTTTGTTCGAGTGGAAAATTGAAGATCTTGCTTATGAATGGCCTAAACAAGCTCTTGGAGACATTGCTTTGGCTATTTCTTGGGTTTTCTTTCTTGTTTATTCTTGGAGAGAGAAGTATGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

150

Amino Acids

17.02

Weight (kDa)

5.88

Isoelectric Point (pI)

24.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7865 PF25266 8 - 138 1.5e-60 Domain of unknown function (DUF7865)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 49
AclWI GGATC 1 cut(s) 163
AfaI GTAC 1 cut(s) 234
AgsI TTSAA 2 cut(s) 114, 350
AhlI ACTAGT 1 cut(s) 74
AjnI CCWGG 1 cut(s) 124
AluBI AGCT 3 cut(s) 110, 276, 380
AluI AGCT 3 cut(s) 110, 276, 380
Alw26I GTCTC 1 cut(s) 381
AlwI GGATC 1 cut(s) 163
AoxI GGCC 1 cut(s) 368
AspLEI GCGC 1 cut(s) 70
AspS9I GGNCC 2 cut(s) 122, 291
AsuNHI GCTAGC 1 cut(s) 140
AvaII GGWCC 2 cut(s) 122, 291
BccI CCATC 1 cut(s) 34
BciT130I CCWGG 1 cut(s) 126
BcoDI GTCTC 1 cut(s) 381
BcuI ACTAGT 1 cut(s) 74
BfaI CTAG 2 cut(s) 75, 141
BfmI CTRYAG 1 cut(s) 6
BglII AGATCT 1 cut(s) 352
Bme1390I CCNGG 1 cut(s) 126
Bme18I GGWCC 2 cut(s) 122, 291
BmgT120I GGNCC 2 cut(s) 122, 291
BmiI GGNNCC 2 cut(s) 82, 292
BmrFI CCNGG 1 cut(s) 126
BmtI GCTAGC 1 cut(s) 144
Bsa29I ATCGAT 1 cut(s) 17
Bse3DI GCAATG 1 cut(s) 390
BseBI CCWGG 1 cut(s) 126
BseCI ATCGAT 1 cut(s) 17
BseMI GCAATG 1 cut(s) 390
BseMII CTCAG 1 cut(s) 206
BshFI GGCC 1 cut(s) 370
BshVI ATCGAT 1 cut(s) 17
BsmAI GTCTC 1 cut(s) 381
BsnI GGCC 1 cut(s) 370
Bsp143I GATC 2 cut(s) 155, 352
BspANI GGCC 1 cut(s) 370
BspCNI CTCAG 1 cut(s) 205
BspDI ATCGAT 1 cut(s) 17
BspLI GGNNCC 2 cut(s) 82, 292
BspOI GCTAGC 1 cut(s) 144
BspPI GGATC 1 cut(s) 163
BsrDI GCAATG 1 cut(s) 390
BssMI GATC 2 cut(s) 155, 352
BssNAI GTATAC 1 cut(s) 50
Bst1107I GTATAC 1 cut(s) 50
Bst2UI CCWGG 1 cut(s) 126
Bst4CI ACNGT 1 cut(s) 237
BstC8I GCNNGC 1 cut(s) 142
BstDEI CTNAG 1 cut(s) 192
BstHHI GCGC 1 cut(s) 70
BstKTI GATC 2 cut(s) 158, 355
BstMAI GTCTC 1 cut(s) 381
BstMBI GATC 2 cut(s) 155, 352
BstNI CCWGG 1 cut(s) 126
BstSCI CCNGG 1 cut(s) 124
BstSFI CTRYAG 1 cut(s) 6
BstX2I RGATCY 1 cut(s) 352
BstYI RGATCY 1 cut(s) 352
BstZ17I GTATAC 1 cut(s) 50
Bsu15I ATCGAT 1 cut(s) 17
BsuRI GGCC 1 cut(s) 370
BsuTUI ATCGAT 1 cut(s) 17
Cac8I GCNNGC 1 cut(s) 142
CfoI GCGC 1 cut(s) 70
Cfr13I GGNCC 2 cut(s) 122, 291
ClaI ATCGAT 1 cut(s) 17
Csp6I GTAC 1 cut(s) 233
CviAII CATG 1 cut(s) 241
CviJI RGCY 6 cut(s) 110, 276, 317, 370, 380, 401
CviKI_1 RGCY 6 cut(s) 110, 276, 317, 370, 380, 401
CviQI GTAC 1 cut(s) 233
DdeI CTNAG 1 cut(s) 192
DpnI GATC 2 cut(s) 157, 354
DpnII GATC 2 cut(s) 155, 352
Eco47I GGWCC 2 cut(s) 122, 291
EcoRII CCWGG 1 cut(s) 124
FaeI CATG 1 cut(s) 244
FaiI YATR 8 cut(s) 50, 182, 242, 273, 306, 320, 363, 447
FatI CATG 1 cut(s) 240
FblI GTMKAC 1 cut(s) 49
FspBI CTAG 2 cut(s) 75, 141
GlaI GCGC 1 cut(s) 69
HaeIII GGCC 1 cut(s) 370
HhaI GCGC 1 cut(s) 70
Hin1II CATG 1 cut(s) 244
Hin6I GCGC 1 cut(s) 68
HinP1I GCGC 1 cut(s) 68
HindIII AAGCTT 1 cut(s) 108
HinfI GANTC 2 cut(s) 22, 298
Hpy166II GTNNAC 1 cut(s) 50
Hpy8I GTNNAC 1 cut(s) 50
HpyCH4III ACNGT 1 cut(s) 237
HpyCH4V TGCA 1 cut(s) 221
HpyF3I CTNAG 1 cut(s) 192
Hsp92II CATG 1 cut(s) 244
HspAI GCGC 1 cut(s) 68
Kzo9I GATC 2 cut(s) 155, 352
LpnPI CCDG 4 cut(s) 111, 138, 189, 270
MaeI CTAG 2 cut(s) 75, 141
MalI GATC 2 cut(s) 157, 354
MboI GATC 2 cut(s) 155, 352
MboII GAAGA 2 cut(s) 271, 362
MfeI CAATTG 1 cut(s) 222
MflI RGATCY 1 cut(s) 352
MluCI AATT 3 cut(s) 63, 222, 345
MnlI CCTC 1 cut(s) 318
MseI TTAA 2 cut(s) 86, 311
MspR9I CCNGG 1 cut(s) 126
MunI CAATTG 1 cut(s) 222
MvaI CCWGG 1 cut(s) 126
NdeII GATC 2 cut(s) 155, 352
NheI GCTAGC 1 cut(s) 140
NlaIII CATG 1 cut(s) 244
NlaIV GGNNCC 2 cut(s) 82, 292
PfeI GAWTC 2 cut(s) 22, 298
Psp6I CCWGG 1 cut(s) 124
PspGI CCWGG 1 cut(s) 124
PspN4I GGNNCC 2 cut(s) 82, 292
PspPI GGNCC 2 cut(s) 122, 291
PsrI GAACNNNNNNTAC 2 cut(s) 165, 197
PsuI RGATCY 1 cut(s) 352
RsaI GTAC 1 cut(s) 234
RsaNI GTAC 1 cut(s) 233
SaqAI TTAA 2 cut(s) 86, 311
Sau3AI GATC 2 cut(s) 155, 352
Sau96I GGNCC 2 cut(s) 122, 291
ScrFI CCNGG 1 cut(s) 126
SetI ASST 4 cut(s) 86, 112, 278, 382
SfcI CTRYAG 1 cut(s) 6
SinI GGWCC 2 cut(s) 122, 291
SpeI ACTAGT 1 cut(s) 74
Sse9I AATT 3 cut(s) 63, 222, 345
SspMI CTAG 2 cut(s) 75, 141
StyD4I CCNGG 1 cut(s) 124
TaaI ACNGT 1 cut(s) 237
TaqI TCGA 4 cut(s) 17, 37, 59, 336
TasI AATT 3 cut(s) 63, 222, 345
TfiI GAWTC 2 cut(s) 22, 298
Tru1I TTAA 2 cut(s) 86, 311
Tru9I TTAA 2 cut(s) 86, 311
TspDTI ATGAA 5 cut(s) 35, 186, 229, 260, 378
TspGWI ACGGA 1 cut(s) 283
VpaK11BI GGWCC 2 cut(s) 122, 291
XmiI GTMKAC 1 cut(s) 49
XspI CTAG 2 cut(s) 75, 141
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.