MD02G1012100.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0003774
Physical Location & Seq
Reverse (-)
770003 .. 770341
339 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1012100.v1.1.491

Sequence Viewer

Length: 339 bp
ATGTTCTACATGACGGAAGTCTACACCTTCGGGCACAGGATTGAGACCGCCCAGAAGCTGCTGGGGTCCACCCCATACGACCTGCTCCTGATTCGGATCGGCGACTCCTTCTCCGGCCTCCTTCTCTTCGCGATCGGCTTCCTGCTGCTCATGGTCTCCTTCGTGAAAGATCGCAAGTTCCAAAGCTTCTTCGCCAAGGGGTGCACGTTGCTCCACGTTTTCATGGCGATGTGGCGGTTCTACTTCAAGCAACGGGTGGAGGACCTCGCCTGGGACTGGCTGAGGCAGACGGTCGGTGACGTCGTGCCGGCGGCGTCTTGGGTTTATGTTAAACCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

113

Amino Acids

13.13

Weight (kDa)

9.17

Isoelectric Point (pI)

41.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7865 PF25266 1 - 108 4.6e-52 Domain of unknown function (DUF7865)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 303
Acc36I ACCTGC 1 cut(s) 90
AccI GTMKAC 1 cut(s) 21
AccII CGCG 1 cut(s) 131
AciI CCGC 3 cut(s) 48, 235, 311
AclWI GGATC 1 cut(s) 104
AcyI GRCGYC 2 cut(s) 300, 314
AfiI CCNNNNNNNGG 2 cut(s) 271, 276
AgsI TTSAA 1 cut(s) 247
AjnI CCWGG 1 cut(s) 269
AluBI AGCT 2 cut(s) 58, 186
AluI AGCT 2 cut(s) 58, 186
Alw21I GWGCWC 1 cut(s) 206
Alw26I GTCTC 2 cut(s) 38, 160
Alw44I GTGCAC 1 cut(s) 202
AlwI GGATC 1 cut(s) 104
AlwNI CAGNNNCTG 1 cut(s) 58
AoxI GGCC 1 cut(s) 115
ApaLI GTGCAC 1 cut(s) 202
ApeKI GCWGC 2 cut(s) 58, 145
AspS9I GGNCC 2 cut(s) 66, 262
AsuHPI GGTGA 1 cut(s) 308
AvaII GGWCC 2 cut(s) 66, 262
BaeGI GKGCMC 2 cut(s) 36, 206
Bbv12I GWGCWC 1 cut(s) 206
BbvCI CCTCAGC 1 cut(s) 281
BbvI GCAGC 2 cut(s) 45, 132
BciT130I CCWGG 1 cut(s) 271
BcoDI GTCTC 2 cut(s) 38, 160
BfuAI ACCTGC 1 cut(s) 90
BisI GCNGC 3 cut(s) 59, 146, 312
BlsI GCNGC 3 cut(s) 60, 147, 313
Bme1390I CCNGG 1 cut(s) 271
Bme18I GGWCC 2 cut(s) 66, 262
BmgT120I GGNCC 2 cut(s) 66, 262
BmiI GGNNCC 1 cut(s) 67
BmrFI CCNGG 1 cut(s) 271
BoxI GACNNNNGTC 1 cut(s) 17
Bpu10I CCTNAGC 1 cut(s) 281
BsaBI GATNNNNATC 1 cut(s) 95
BsaHI GRCGYC 2 cut(s) 300, 314
BsaI GGTCTC 2 cut(s) 38, 160
BsaJI CCNNGG 2 cut(s) 195, 270
BsaXI ACNNNNNCTCC 2 cut(s) 95, 125
Bsc4I CCNNNNNNNGG 2 cut(s) 271, 276
Bse118I RCCGGY 1 cut(s) 307
Bse1I ACTGG 1 cut(s) 281
Bse8I GATNNNNATC 1 cut(s) 95
BseBI CCWGG 1 cut(s) 271
BseDI CCNNGG 2 cut(s) 195, 270
BseJI GATNNNNATC 1 cut(s) 95
BseLI CCNNNNNNNGG 2 cut(s) 271, 276
BseMII CTCAG 1 cut(s) 272
BseNI ACTGG 1 cut(s) 281
BseSI GKGCMC 2 cut(s) 36, 206
BseXI GCAGC 2 cut(s) 45, 132
BseYI CCCAGC 1 cut(s) 61
Bsh1236I CGCG 1 cut(s) 131
Bsh1285I CGRYCG 2 cut(s) 135, 294
BshFI GGCC 1 cut(s) 117
BsiEI CGRYCG 2 cut(s) 135, 294
BsiHKAI GWGCWC 1 cut(s) 206
BsiSI CCGG 2 cut(s) 114, 308
BslFI GGGAC 1 cut(s) 287
BslI CCNNNNNNNGG 2 cut(s) 271, 276
BsmAI GTCTC 2 cut(s) 38, 160
BsmFI GGGAC 1 cut(s) 287
BsnI GGCC 1 cut(s) 117
Bso31I GGTCTC 2 cut(s) 38, 160
Bsp1286I GDGCHC 2 cut(s) 36, 206
Bsp143I GATC 3 cut(s) 96, 132, 169
Bsp68I TCGCGA 1 cut(s) 131
BspACI CCGC 3 cut(s) 48, 235, 311
BspANI GGCC 1 cut(s) 117
BspCNI CTCAG 1 cut(s) 273
BspFNI CGCG 1 cut(s) 131
BspLI GGNNCC 1 cut(s) 67
BspMI ACCTGC 1 cut(s) 90
BspPI GGATC 1 cut(s) 104
BspTNI GGTCTC 2 cut(s) 38, 160
BsrFI RCCGGY 1 cut(s) 307
BsrI ACTGG 1 cut(s) 281
BssAI RCCGGY 1 cut(s) 307
BssECI CCNNGG 2 cut(s) 195, 270
BssMI GATC 3 cut(s) 96, 132, 169
BssNI GRCGYC 2 cut(s) 300, 314
BssT1I CCWWGG 1 cut(s) 195
Bst2UI CCWGG 1 cut(s) 271
Bst4CI ACNGT 1 cut(s) 292
Bst6I CTCTTC 1 cut(s) 131
BstACI GRCGYC 2 cut(s) 300, 314
BstC8I GCNNGC 1 cut(s) 309
BstDEI CTNAG 1 cut(s) 281
BstFNI CGCG 1 cut(s) 131
BstKTI GATC 3 cut(s) 99, 135, 172
BstMAI GTCTC 2 cut(s) 38, 160
BstMBI GATC 3 cut(s) 96, 132, 169
BstMCI CGRYCG 2 cut(s) 135, 294
BstNI CCWGG 1 cut(s) 271
BstPAI GACNNNNGTC 1 cut(s) 17
BstSCI CCNGG 1 cut(s) 269
BstSLI GKGCMC 2 cut(s) 36, 206
BstUI CGCG 1 cut(s) 131
BstV1I GCAGC 2 cut(s) 45, 132
BsuRI GGCC 1 cut(s) 117
BtgZI GCGATG 1 cut(s) 242
BtuMI TCGCGA 1 cut(s) 131
BveI ACCTGC 1 cut(s) 90
Cac8I GCNNGC 1 cut(s) 309
CaiI CAGNNNCTG 1 cut(s) 58
Cfr10I RCCGGY 1 cut(s) 307
Cfr13I GGNCC 2 cut(s) 66, 262
CseI GACGC 1 cut(s) 303
CviAII CATG 3 cut(s) 10, 151, 223
CviJI RGCY 5 cut(s) 58, 117, 138, 186, 280
CviKI_1 RGCY 5 cut(s) 58, 117, 138, 186, 280
DdeI CTNAG 1 cut(s) 281
DpnI GATC 3 cut(s) 98, 134, 171
DpnII GATC 3 cut(s) 96, 132, 169
Eam1104I CTCTTC 1 cut(s) 131
EarI CTCTTC 1 cut(s) 131
Eco130I CCWWGG 1 cut(s) 195
Eco31I GGTCTC 2 cut(s) 38, 160
Eco47I GGWCC 2 cut(s) 66, 262
EcoO109I RGGNCCY 1 cut(s) 262
EcoRII CCWGG 1 cut(s) 269
EcoT14I CCWWGG 1 cut(s) 195
ErhI CCWWGG 1 cut(s) 195
FaeI CATG 3 cut(s) 13, 154, 226
FaiI YATR 6 cut(s) 11, 76, 152, 224, 327, 337
FaqI GGGAC 1 cut(s) 287
FatI CATG 3 cut(s) 9, 150, 222
FblI GTMKAC 1 cut(s) 21
Fnu4HI GCNGC 3 cut(s) 59, 146, 312
Fsp4HI GCNGC 3 cut(s) 59, 146, 312
GluI GCNGC 3 cut(s) 59, 146, 312
GsaI CCCAGC 1 cut(s) 65
HaeIII GGCC 1 cut(s) 117
HapII CCGG 2 cut(s) 114, 308
HgaI GACGC 1 cut(s) 303
Hin1I GRCGYC 2 cut(s) 300, 314
Hin1II CATG 3 cut(s) 13, 154, 226
HindIII AAGCTT 1 cut(s) 184
HinfI GANTC 2 cut(s) 91, 104
HpaII CCGG 2 cut(s) 114, 308
HphI GGTGA 1 cut(s) 308
Hpy166II GTNNAC 3 cut(s) 22, 69, 204
Hpy188I TCNGA 1 cut(s) 96
Hpy188III TCNNGA 3 cut(s) 88, 130, 163
Hpy8I GTNNAC 3 cut(s) 22, 69, 204
Hpy99I CGWCG 1 cut(s) 305
HpyAV CCTTC 4 cut(s) 37, 118, 131, 169
HpyCH4III ACNGT 1 cut(s) 292
HpyCH4IV ACGT 3 cut(s) 206, 216, 300
HpyCH4V TGCA 1 cut(s) 204
HpyF3I CTNAG 1 cut(s) 281
HpySE526I ACGT 3 cut(s) 206, 216, 300
Hsp92I GRCGYC 2 cut(s) 300, 314
Hsp92II CATG 3 cut(s) 13, 154, 226
KroI GCCGGC 1 cut(s) 307
KroNI GCCGGC 1 cut(s) 309
Kzo9I GATC 3 cut(s) 96, 132, 169
LmnI GCTCC 2 cut(s) 90, 216
Lsp1109I GCAGC 2 cut(s) 45, 132
MaeII ACGT 3 cut(s) 206, 216, 300
MaeIII GTNAC 1 cut(s) 296
MalI GATC 3 cut(s) 98, 134, 171
MboI GATC 3 cut(s) 96, 132, 169
MboII GAAGA 2 cut(s) 118, 181
MhlI GDGCHC 2 cut(s) 36, 206
MlyI GAGTC 1 cut(s) 98
MnlI CCTC 4 cut(s) 128, 253, 275, 276
MroNI GCCGGC 1 cut(s) 307
MseI TTAA 1 cut(s) 330
MslI CAYNNNNRTG 1 cut(s) 227
MspI CCGG 2 cut(s) 114, 308
MspR9I CCNGG 1 cut(s) 271
MvaI CCWGG 1 cut(s) 271
MvnI CGCG 1 cut(s) 131
NaeI GCCGGC 1 cut(s) 309
NdeII GATC 3 cut(s) 96, 132, 169
NgoMIV GCCGGC 1 cut(s) 307
NlaIII CATG 3 cut(s) 13, 154, 226
NlaIV GGNNCC 1 cut(s) 67
NmuCI GTSAC 1 cut(s) 296
NruI TCGCGA 1 cut(s) 131
PcsI WCGNNNNNNNCGW 1 cut(s) 300
PdiI GCCGGC 1 cut(s) 309
PfeI GAWTC 1 cut(s) 91
PkrI GCNGC 3 cut(s) 60, 147, 313
Ple19I CGATCG 1 cut(s) 135
PleI GAGTC 1 cut(s) 98
PpsI GAGTC 1 cut(s) 98
PpuMI RGGWCCY 1 cut(s) 262
PshAI GACNNNNGTC 1 cut(s) 17
Psp5II RGGWCCY 1 cut(s) 262
Psp6I CCWGG 1 cut(s) 269
PspFI CCCAGC 1 cut(s) 61
PspGI CCWGG 1 cut(s) 269
PspN4I GGNNCC 1 cut(s) 67
PspPI GGNCC 2 cut(s) 66, 262
PspPPI RGGWCCY 1 cut(s) 262
PstNI CAGNNNCTG 1 cut(s) 58
PvuI CGATCG 1 cut(s) 135
RruI TCGCGA 1 cut(s) 131
RseI CAYNNNNRTG 1 cut(s) 227
SaqAI TTAA 1 cut(s) 330
SatI GCNGC 3 cut(s) 59, 146, 312
Sau3AI GATC 3 cut(s) 96, 132, 169
Sau96I GGNCC 2 cut(s) 66, 262
SchI GAGTC 1 cut(s) 98
ScrFI CCNGG 1 cut(s) 271
SduI GDGCHC 2 cut(s) 36, 206
SetI ASST 8 cut(s) 29, 60, 84, 188, 209, 219, 267, 303
SinI GGWCC 2 cut(s) 66, 262
SmiMI CAYNNNNRTG 1 cut(s) 227
SsiI CCGC 3 cut(s) 48, 235, 311
StyD4I CCNGG 1 cut(s) 269
StyI CCWWGG 1 cut(s) 195
TaaI ACNGT 1 cut(s) 292
TaiI ACGT 3 cut(s) 209, 219, 303
TauI GCSGC 1 cut(s) 314
TfiI GAWTC 1 cut(s) 91
Tru1I TTAA 1 cut(s) 330
Tru9I TTAA 1 cut(s) 330
TseFI GTSAC 1 cut(s) 296
TseI GCWGC 2 cut(s) 58, 145
Tsp45I GTSAC 1 cut(s) 296
TspDTI ATGAA 1 cut(s) 211
TspGWI ACGGA 1 cut(s) 29
VneI GTGCAC 1 cut(s) 202
VpaK11BI GGWCC 2 cut(s) 66, 262
XmiI GTMKAC 1 cut(s) 21
ZraI GACGTC 1 cut(s) 301
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.