FvH4_3g09112

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0003774
Physical Location & Seq
Forward (+)
5342148 .. 5347122
4975 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g09112.t1

Sequence Viewer

Length: 756 bp
ATGCCACCGCCGTCGTCGCCGTCGACGTCATGGCCGTTCACCGTGATCTGCCTCCTCCACTCGGCGATCGCGATCGCGTGCGGCGCTTGCATGATGTTCTACATGACGGATCTCTACACCTTCGGCCACGGCGTCGAGACGGCGCAGAAGCTCCTCGGCTCGACGCCGCACGACCAGCTCCTGATCCGGACCTCCGACTCGTTCTCCGGCTTGCTCCTCTTCGCGATCGGCTTCCTTCTCCTCATGGTCTCCTTCGTCAGAGATCGCGAGTTCCAGAGCTTCTTCGCCAAGGGCTGCACGGTCCTCCATGTCTTCATGGGACTCTGGCGGTGCTACTTCGAGCGGAGAGTCGAGGATCTCGCCTGGGATTGGCTCCGCCAGACAGTCGGCGACGTCGTTTTGGCCCTCTCTTGGGTTTTCTTCCTCGTGTACTCGTGGAGAGAAAAGAAGCCCCTCGAGGTCAAAGCGCCACCAGTCTTGATATGCAGCACAACTGATCTCCATGCTATGTTTGCCAGCAGTGAAAGTAGACTCGATTATTCCTTCAGAGTTAATCAGAAACTGACGAGCCAATGCATTTATCTTCATTGTGTATCGCATGTGAGGTTTGAGAAGCCTGTAGATGGGGTGCATCACACTCAGCTGCTGGAATCATTGGAGCATACGTGGGCTTTGCCAAGTTCCCAAAGCCAATTTGTTGTAGCATCGATTGGAGGAGTGAGAACCTGCTTAGATGGTGAATCTGAATCGGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

252

Amino Acids

28.39

Weight (kDa)

6.18

Isoelectric Point (pI)

45.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7865 PF25266 11 - 139 3.6e-67 Domain of unknown function (DUF7865)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 29, 396
AbsI CCTCGAGG 1 cut(s) 455
Acc36I ACCTGC 1 cut(s) 734
AccBSI CCGCTC 1 cut(s) 343
AccI GTMKAC 2 cut(s) 23, 529
AccII CGCG 4 cut(s) 71, 77, 224, 267
AccIII TCCGGA 1 cut(s) 186
AciI CCGC 6 cut(s) 8, 81, 167, 328, 343, 376
AclWI GGATC 3 cut(s) 117, 178, 363
AcoI YGGCCR 2 cut(s) 32, 124
AcuI CTGAAG 1 cut(s) 529
AcyI GRCGYC 4 cut(s) 26, 132, 164, 393
AfaI GTAC 1 cut(s) 431
AfiI CCNNNNNNNGG 5 cut(s) 61, 369, 411, 412, 623
AjnI CCWGG 1 cut(s) 362
AluBI AGCT 4 cut(s) 151, 178, 279, 643
AluI AGCT 4 cut(s) 151, 178, 279, 643
Alw26I GTCTC 2 cut(s) 131, 253
AlwI GGATC 3 cut(s) 117, 178, 363
AlwNI CAGNNNCTG 3 cut(s) 181, 562, 646
Ama87I CYCGRG 1 cut(s) 455
Aor13HI TCCGGA 1 cut(s) 186
AoxI GGCC 3 cut(s) 32, 124, 402
ApeKI GCWGC 3 cut(s) 294, 486, 643
AsiSI GCGATCGC 2 cut(s) 69, 75
AspLEI GCGC 3 cut(s) 86, 145, 469
AspS9I GGNCC 3 cut(s) 189, 301, 403
AsuHPI GGTGA 2 cut(s) 31, 749
AvaI CYCGRG 1 cut(s) 455
AvaII GGWCC 2 cut(s) 189, 301
BauI CACGAG 2 cut(s) 425, 433
BbsI GAAGAC 1 cut(s) 304
BbvI GCAGC 3 cut(s) 281, 498, 630
BccI CCATC 2 cut(s) 617, 728
BceAI ACGGC 4 cut(s) 4, 19, 145, 156
BciT130I CCWGG 1 cut(s) 364
BcoDI GTCTC 2 cut(s) 131, 253
BfmI CTRYAG 1 cut(s) 618
BfoI RGCGCY 2 cut(s) 87, 470
BfuAI ACCTGC 1 cut(s) 734
BisI GCNGC 5 cut(s) 82, 167, 295, 487, 644
BlsI GCNGC 5 cut(s) 83, 168, 296, 488, 645
Bme1390I CCNGG 1 cut(s) 364
Bme18I GGWCC 2 cut(s) 189, 301
BmeT110I CYCGRG 1 cut(s) 455
BmgT120I GGNCC 3 cut(s) 189, 301, 403
BmiI GGNNCC 1 cut(s) 374
BmrFI CCNGG 1 cut(s) 364
BmsI GCATC 2 cut(s) 640, 713
BpiI GAAGAC 1 cut(s) 304
Bsa29I ATCGAT 1 cut(s) 707
BsaAI YACGTR 1 cut(s) 666
BsaBI GATNNNNATC 1 cut(s) 71
BsaHI GRCGYC 4 cut(s) 26, 132, 164, 393
BsaI GGTCTC 1 cut(s) 253
BsaJI CCNNGG 4 cut(s) 127, 154, 288, 363
BsaWI WCCGGW 1 cut(s) 186
BsaXI ACNNNNNCTCC 4 cut(s) 188, 218, 650, 680
Bsc4I CCNNNNNNNGG 5 cut(s) 61, 369, 411, 412, 623
Bse1I ACTGG 1 cut(s) 473
Bse8I GATNNNNATC 1 cut(s) 71
BseAI TCCGGA 1 cut(s) 186
BseBI CCWGG 1 cut(s) 364
BseCI ATCGAT 1 cut(s) 707
BseDI CCNNGG 4 cut(s) 127, 154, 288, 363
BseJI GATNNNNATC 1 cut(s) 71
BseLI CCNNNNNNNGG 5 cut(s) 61, 369, 411, 412, 623
BseMII CTCAG 1 cut(s) 653
BseNI ACTGG 1 cut(s) 473
BseRI GAGGAG 5 cut(s) 44, 143, 206, 230, 729
BseXI GCAGC 3 cut(s) 281, 498, 630
BsgI GTGCAG 1 cut(s) 280
Bsh1236I CGCG 4 cut(s) 71, 77, 224, 267
Bsh1285I CGRYCG 3 cut(s) 69, 75, 228
BshFI GGCC 3 cut(s) 34, 126, 404
BshVI ATCGAT 1 cut(s) 707
BsiEI CGRYCG 3 cut(s) 69, 75, 228
BsiHKCI CYCGRG 1 cut(s) 455
BsiSI CCGG 2 cut(s) 187, 207
BslFI GGGAC 1 cut(s) 333
BslI CCNNNNNNNGG 5 cut(s) 61, 369, 411, 412, 623
BsmAI GTCTC 2 cut(s) 131, 253
BsmBI CGTCTC 1 cut(s) 131
BsmFI GGGAC 1 cut(s) 333
BsnI GGCC 3 cut(s) 34, 126, 404
Bso31I GGTCTC 1 cut(s) 253
BsoBI CYCGRG 1 cut(s) 455
Bsp13I TCCGGA 1 cut(s) 186
Bsp143I GATC 9 cut(s) 45, 66, 72, 109, 183, 225, 262, 355, 496
Bsp68I TCGCGA 3 cut(s) 71, 224, 267
BspACI CCGC 6 cut(s) 8, 81, 167, 328, 343, 376
BspANI GGCC 3 cut(s) 34, 126, 404
BspCNI CTCAG 1 cut(s) 652
BspDI ATCGAT 1 cut(s) 707
BspEI TCCGGA 1 cut(s) 186
BspFNI CGCG 4 cut(s) 71, 77, 224, 267
BspLI GGNNCC 1 cut(s) 374
BspMI ACCTGC 1 cut(s) 734
BspPI GGATC 3 cut(s) 117, 178, 363
BspTNI GGTCTC 1 cut(s) 253
BsrBI CCGCTC 1 cut(s) 343
BsrI ACTGG 1 cut(s) 473
BssECI CCNNGG 4 cut(s) 127, 154, 288, 363
BssMI GATC 9 cut(s) 45, 66, 72, 109, 183, 225, 262, 355, 496
BssNI GRCGYC 4 cut(s) 26, 132, 164, 393
BssSI CACGAG 2 cut(s) 425, 433
BssT1I CCWWGG 1 cut(s) 288
Bst2BI CACGAG 2 cut(s) 425, 433
Bst2UI CCWGG 1 cut(s) 364
Bst4CI ACNGT 3 cut(s) 43, 301, 385
Bst6I CTCTTC 1 cut(s) 224
BstACI GRCGYC 4 cut(s) 26, 132, 164, 393
BstBAI YACGTR 1 cut(s) 666
BstC8I GCNNGC 4 cut(s) 79, 88, 212, 517
BstDEI CTNAG 2 cut(s) 639, 730
BstDSI CCRYGG 1 cut(s) 127
BstFNI CGCG 4 cut(s) 71, 77, 224, 267
BstH2I RGCGCY 2 cut(s) 87, 470
BstHHI GCGC 3 cut(s) 86, 145, 469
BstKTI GATC 9 cut(s) 48, 69, 75, 112, 186, 228, 265, 358, 499
BstMAI GTCTC 2 cut(s) 131, 253
BstMBI GATC 9 cut(s) 45, 66, 72, 109, 183, 225, 262, 355, 496
BstMCI CGRYCG 3 cut(s) 69, 75, 228
BstMWI GCNNNNNNNGC 5 cut(s) 16, 83, 87, 175, 512
BstNI CCWGG 1 cut(s) 364
BstNSI RCATGY 1 cut(s) 602
BstSCI CCNGG 1 cut(s) 362
BstSFI CTRYAG 1 cut(s) 618
BstUI CGCG 4 cut(s) 71, 77, 224, 267
BstV1I GCAGC 3 cut(s) 281, 498, 630
BstV2I GAAGAC 1 cut(s) 304
BstX2I RGATCY 2 cut(s) 109, 355
BstYI RGATCY 2 cut(s) 109, 355
Bsu15I ATCGAT 1 cut(s) 707
BsuRI GGCC 3 cut(s) 34, 126, 404
BsuTUI ATCGAT 1 cut(s) 707
BtgI CCRYGG 1 cut(s) 127
BtsI GCAGTG 1 cut(s) 526
BtsIMutI CAGTG 1 cut(s) 526
BtuMI TCGCGA 3 cut(s) 71, 224, 267
BveI ACCTGC 1 cut(s) 734
Cac8I GCNNGC 4 cut(s) 79, 88, 212, 517
CaiI CAGNNNCTG 3 cut(s) 181, 562, 646
CfoI GCGC 3 cut(s) 86, 145, 469
Cfr13I GGNCC 3 cut(s) 189, 301, 403
ClaI ATCGAT 1 cut(s) 707
CseI GACGC 2 cut(s) 121, 172
Csp6I GTAC 1 cut(s) 430
CspCI CAANNNNNGTGG 2 cut(s) 459, 494
CviAII CATG 8 cut(s) 30, 91, 103, 244, 308, 316, 503, 599
CviQI GTAC 1 cut(s) 430
DdeI CTNAG 2 cut(s) 639, 730
DpnI GATC 9 cut(s) 47, 68, 74, 111, 185, 227, 264, 357, 498
DpnII GATC 9 cut(s) 45, 66, 72, 109, 183, 225, 262, 355, 496
EaeI YGGCCR 2 cut(s) 32, 124
Eam1104I CTCTTC 1 cut(s) 224
EarI CTCTTC 1 cut(s) 224
EciI GGCGGA 1 cut(s) 365
Eco130I CCWWGG 1 cut(s) 288
Eco31I GGTCTC 1 cut(s) 253
Eco47I GGWCC 2 cut(s) 189, 301
Eco57I CTGAAG 1 cut(s) 529
Eco88I CYCGRG 1 cut(s) 455
EcoRII CCWGG 1 cut(s) 362
EcoT14I CCWWGG 1 cut(s) 288
EcoT22I ATGCAT 1 cut(s) 578
ErhI CCWWGG 1 cut(s) 288
Esp3I CGTCTC 1 cut(s) 131
FaeI CATG 8 cut(s) 33, 94, 106, 247, 311, 319, 506, 602
FaqI GGGAC 1 cut(s) 333
FatI CATG 8 cut(s) 29, 90, 102, 243, 307, 315, 502, 598
FblI GTMKAC 2 cut(s) 23, 529
Fnu4HI GCNGC 5 cut(s) 82, 167, 295, 487, 644
Fsp4HI GCNGC 5 cut(s) 82, 167, 295, 487, 644
GlaI GCGC 3 cut(s) 85, 144, 468
GluI GCNGC 5 cut(s) 82, 167, 295, 487, 644
HaeII RGCGCY 2 cut(s) 87, 470
HaeIII GGCC 3 cut(s) 34, 126, 404
HapII CCGG 2 cut(s) 187, 207
HgaI GACGC 2 cut(s) 121, 172
HhaI GCGC 3 cut(s) 86, 145, 469
Hin1I GRCGYC 4 cut(s) 26, 132, 164, 393
Hin1II CATG 8 cut(s) 33, 94, 106, 247, 311, 319, 506, 602
Hin6I GCGC 3 cut(s) 84, 143, 467
HinP1I GCGC 3 cut(s) 84, 143, 467
HincII GTYRAC 1 cut(s) 24
HindII GTYRAC 1 cut(s) 24
HinfI GANTC 7 cut(s) 197, 321, 348, 531, 650, 740, 746
HpaII CCGG 2 cut(s) 187, 207
HphI GGTGA 2 cut(s) 31, 749
Hpy166II GTNNAC 4 cut(s) 24, 39, 430, 530
Hpy188I TCNGA 5 cut(s) 196, 260, 548, 558, 745
Hpy188III TCNNGA 8 cut(s) 70, 136, 181, 187, 223, 266, 274, 478
Hpy8I GTNNAC 4 cut(s) 24, 39, 430, 530
Hpy99I CGWCG 8 cut(s) 16, 19, 25, 28, 137, 166, 395, 398
HpyAV CCTTC 4 cut(s) 130, 245, 262, 553
HpyCH4III ACNGT 3 cut(s) 43, 301, 385
HpyCH4IV ACGT 3 cut(s) 26, 393, 665
HpyCH4V TGCA 5 cut(s) 90, 297, 486, 576, 631
HpyF10VI GCNNNNNNNGC 5 cut(s) 16, 83, 87, 175, 512
HpyF3I CTNAG 2 cut(s) 639, 730
HpySE526I ACGT 3 cut(s) 26, 393, 665
Hsp92I GRCGYC 4 cut(s) 26, 132, 164, 393
Hsp92II CATG 8 cut(s) 33, 94, 106, 247, 311, 319, 506, 602
HspAI GCGC 3 cut(s) 84, 143, 467
Kpn2I TCCGGA 1 cut(s) 186
Kzo9I GATC 9 cut(s) 45, 66, 72, 109, 183, 225, 262, 355, 496
LmnI GCTCC 5 cut(s) 156, 183, 219, 378, 658
Lsp1109I GCAGC 3 cut(s) 281, 498, 630
LweI GCATC 2 cut(s) 640, 713
MaeII ACGT 3 cut(s) 26, 393, 665
MalI GATC 9 cut(s) 47, 68, 74, 111, 185, 227, 264, 357, 498
MbiI CCGCTC 1 cut(s) 343
MboI GATC 9 cut(s) 45, 66, 72, 109, 183, 225, 262, 355, 496
MboII GAAGA 5 cut(s) 211, 274, 304, 412, 575
MflI RGATCY 2 cut(s) 109, 355
MluCI AATT 1 cut(s) 692
MlyI GAGTC 4 cut(s) 191, 315, 357, 525
MmeI TCCRAC 1 cut(s) 219
Mph1103I ATGCAT 1 cut(s) 578
MroI TCCGGA 1 cut(s) 186
MseI TTAA 1 cut(s) 552
MspA1I CMGCKG 1 cut(s) 643
MspI CCGG 2 cut(s) 187, 207
MspR9I CCNGG 1 cut(s) 364
MvaI CCWGG 1 cut(s) 364
MvnI CGCG 4 cut(s) 71, 77, 224, 267
MwoI GCNNNNNNNGC 5 cut(s) 16, 83, 87, 175, 512
NdeII GATC 9 cut(s) 45, 66, 72, 109, 183, 225, 262, 355, 496
NlaIII CATG 8 cut(s) 33, 94, 106, 247, 311, 319, 506, 602
NlaIV GGNNCC 1 cut(s) 374
NmeAIII GCCGAG 2 cut(s) 41, 135
NruI TCGCGA 3 cut(s) 71, 224, 267
NsiI ATGCAT 1 cut(s) 578
NspI RCATGY 1 cut(s) 602
PaeR7I CTCGAG 1 cut(s) 455
PcsI WCGNNNNNNNCGW 6 cut(s) 20, 23, 32, 68, 129, 393
PfeI GAWTC 3 cut(s) 650, 740, 746
PkrI GCNGC 5 cut(s) 83, 168, 296, 488, 645
Ple19I CGATCG 3 cut(s) 69, 75, 228
PleI GAGTC 4 cut(s) 191, 315, 356, 525
PpsI GAGTC 4 cut(s) 191, 315, 356, 525
Ppu21I YACGTR 1 cut(s) 666
Psp6I CCWGG 1 cut(s) 362
PspGI CCWGG 1 cut(s) 362
PspN4I GGNNCC 1 cut(s) 374
PspPI GGNCC 3 cut(s) 189, 301, 403
PspXI VCTCGAGB 1 cut(s) 455
PstNI CAGNNNCTG 3 cut(s) 181, 562, 646
PsuI RGATCY 2 cut(s) 109, 355
PvuI CGATCG 3 cut(s) 69, 75, 228
PvuII CAGCTG 1 cut(s) 643
RgaI GCGATCGC 2 cut(s) 69, 75
RruI TCGCGA 3 cut(s) 71, 224, 267
RsaI GTAC 1 cut(s) 431
RsaNI GTAC 1 cut(s) 430
SalI GTCGAC 1 cut(s) 22
SaqAI TTAA 1 cut(s) 552
SatI GCNGC 5 cut(s) 82, 167, 295, 487, 644
Sau3AI GATC 9 cut(s) 45, 66, 72, 109, 183, 225, 262, 355, 496
Sau96I GGNCC 3 cut(s) 189, 301, 403
SchI GAGTC 4 cut(s) 191, 315, 357, 525
ScrFI CCNGG 1 cut(s) 364
SfaAI GCGATCGC 2 cut(s) 69, 75
SfaNI GCATC 2 cut(s) 640, 713
SfcI CTRYAG 1 cut(s) 618
Sfr274I CTCGAG 1 cut(s) 455
SgfI GCGATCGC 2 cut(s) 69, 75
SgrDI CGTCGACG 1 cut(s) 22
SinI GGWCC 2 cut(s) 189, 301
SlaI CTCGAG 1 cut(s) 455
SmlI CTYRAG 1 cut(s) 455
SmoI CTYRAG 1 cut(s) 455
Sse9I AATT 1 cut(s) 692
SsiI CCGC 6 cut(s) 8, 81, 167, 328, 343, 376
StyD4I CCNGG 1 cut(s) 362
StyI CCWWGG 1 cut(s) 288
TaaI ACNGT 3 cut(s) 43, 301, 385
TaiI ACGT 3 cut(s) 29, 396, 668
TaqI TCGA 8 cut(s) 23, 135, 161, 339, 351, 456, 534, 707
TasI AATT 1 cut(s) 692
TatI WGTACW 1 cut(s) 429
TauI GCSGC 2 cut(s) 84, 169
TfiI GAWTC 3 cut(s) 650, 740, 746
Tru1I TTAA 1 cut(s) 552
Tru9I TTAA 1 cut(s) 552
TscAI CASTG 1 cut(s) 526
TseI GCWGC 3 cut(s) 294, 486, 643
TspDTI ATGAA 2 cut(s) 304, 575
TspGWI ACGGA 1 cut(s) 122
TspRI CASTG 1 cut(s) 526
VpaK11BI GGWCC 2 cut(s) 189, 301
XceI RCATGY 1 cut(s) 602
XhoI CTCGAG 1 cut(s) 455
XmiI GTMKAC 2 cut(s) 23, 529
ZraI GACGTC 2 cut(s) 27, 394
Zsp2I ATGCAT 1 cut(s) 578
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.