MD03G1227700.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0003774
Physical Location & Seq
Reverse (-)
31318242 .. 31318676
435 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1227700.v1.1.491

Sequence Viewer

Length: 435 bp
ATGGGGTTATCTGGGTTTTTCCTAATATGTATGCTCCACTCTGTTGTTGCTTTGATTTGTGGAGCTTTGATGATGTTTTACTCTTATGAATTCTCTGTGTTCATCCATGGCCATGATACAGCAAGCAAGCTCCAAGGATCAACACCTCACGACCGGCTTTTGATCCGAACCGCAGATTCCTTCTCCGGTCTGCTCCTATTTGCCATTGGATTCCTTCTATTCATGGTTGCTTTTGTCAAGGACAACAAGTTCCAGAGCTTCTTTGCCAAGGGTTGTGTGTTGCTTCACATTTCTATGGCCATTTGGAGAGTTTACTTTGAAAGGGACATTCAAGACCTAGCCGGAGACTGGCCAAAACAGGTCGCCGGCGATATCACATTGGCGCTTTCGTGGGCATTACTTCTTGTGTACTCATGGAGGGAGAAGTATGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

145

Amino Acids

16.38

Weight (kDa)

6.49

Isoelectric Point (pI)

23.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7865 PF25266 1 - 131 9.2e-67 Domain of unknown function (DUF7865)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 171
AclWI GGATC 2 cut(s) 145, 157
AcoI YGGCCR 3 cut(s) 109, 297, 350
AcsI RAATTY 1 cut(s) 89
AfaI GTAC 1 cut(s) 410
AfiI CCNNNNNNNGG 1 cut(s) 348
AgsI TTSAA 2 cut(s) 320, 332
AloI GAACNNNNNNTCC 2 cut(s) 233, 265
AluBI AGCT 3 cut(s) 65, 130, 258
AluI AGCT 3 cut(s) 65, 130, 258
Alw26I GTCTC 1 cut(s) 339
AlwI GGATC 2 cut(s) 145, 157
AoxI GGCC 3 cut(s) 109, 297, 350
ApoI RAATTY 1 cut(s) 89
AspLEI GCGC 1 cut(s) 385
BalI TGGCCA 3 cut(s) 111, 299, 352
BcoDI GTCTC 1 cut(s) 339
BfaI CTAG 1 cut(s) 338
BfoI RGCGCY 1 cut(s) 386
BsaJI CCNNGG 3 cut(s) 106, 133, 267
BsaWI WCCGGW 1 cut(s) 185
Bsc4I CCNNNNNNNGG 1 cut(s) 348
Bse118I RCCGGY 2 cut(s) 153, 365
Bse1I ACTGG 1 cut(s) 353
BseDI CCNNGG 3 cut(s) 106, 133, 267
BseGI GGATG 1 cut(s) 102
BseLI CCNNNNNNNGG 1 cut(s) 348
BseNI ACTGG 1 cut(s) 353
Bsh1285I CGRYCG 1 cut(s) 154
BshFI GGCC 3 cut(s) 111, 299, 352
BsiEI CGRYCG 1 cut(s) 154
BsiSI CCGG 4 cut(s) 154, 186, 342, 366
BslFI GGGAC 1 cut(s) 338
BslI CCNNNNNNNGG 1 cut(s) 348
BsmAI GTCTC 1 cut(s) 339
BsmFI GGGAC 1 cut(s) 338
BsnI GGCC 3 cut(s) 111, 299, 352
Bsp143I GATC 2 cut(s) 137, 162
Bsp19I CCATGG 1 cut(s) 106
BspACI CCGC 1 cut(s) 171
BspANI GGCC 3 cut(s) 111, 299, 352
BspPI GGATC 2 cut(s) 145, 157
BsrFI RCCGGY 2 cut(s) 153, 365
BsrI ACTGG 1 cut(s) 353
BssAI RCCGGY 2 cut(s) 153, 365
BssECI CCNNGG 3 cut(s) 106, 133, 267
BssMI GATC 2 cut(s) 137, 162
BssT1I CCWWGG 3 cut(s) 106, 133, 267
BstC8I GCNNGC 3 cut(s) 124, 128, 367
BstDSI CCRYGG 1 cut(s) 106
BstF5I GGATG 1 cut(s) 102
BstH2I RGCGCY 1 cut(s) 386
BstHHI GCGC 1 cut(s) 385
BstKTI GATC 2 cut(s) 140, 165
BstMAI GTCTC 1 cut(s) 339
BstMBI GATC 2 cut(s) 137, 162
BstMCI CGRYCG 1 cut(s) 154
BsuRI GGCC 3 cut(s) 111, 299, 352
BtgI CCRYGG 1 cut(s) 106
BtsCI GGATG 1 cut(s) 102
Cac8I GCNNGC 3 cut(s) 124, 128, 367
CfoI GCGC 1 cut(s) 385
Cfr10I RCCGGY 2 cut(s) 153, 365
Csp6I GTAC 1 cut(s) 409
CviAII CATG 4 cut(s) 107, 113, 223, 414
CviJI RGCY 8 cut(s) 65, 111, 130, 157, 258, 299, 341, 352
CviKI_1 RGCY 8 cut(s) 65, 111, 130, 157, 258, 299, 341, 352
CviQI GTAC 1 cut(s) 409
DpnI GATC 2 cut(s) 139, 164
DpnII GATC 2 cut(s) 137, 162
EaeI YGGCCR 3 cut(s) 109, 297, 350
Eco130I CCWWGG 3 cut(s) 106, 133, 267
Eco32I GATATC 1 cut(s) 373
EcoRI GAATTC 1 cut(s) 89
EcoRV GATATC 1 cut(s) 373
EcoT14I CCWWGG 3 cut(s) 106, 133, 267
ErhI CCWWGG 3 cut(s) 106, 133, 267
FaeI CATG 4 cut(s) 110, 116, 226, 417
FaiI YATR 9 cut(s) 28, 32, 87, 108, 114, 224, 296, 415, 429
FaqI GGGAC 1 cut(s) 338
FatI CATG 4 cut(s) 106, 112, 222, 413
FokI GGATG 1 cut(s) 89
FspBI CTAG 1 cut(s) 338
GlaI GCGC 1 cut(s) 384
HaeII RGCGCY 1 cut(s) 386
HaeIII GGCC 3 cut(s) 111, 299, 352
HapII CCGG 4 cut(s) 154, 186, 342, 366
HhaI GCGC 1 cut(s) 385
Hin1II CATG 4 cut(s) 110, 116, 226, 417
Hin6I GCGC 1 cut(s) 383
HinP1I GCGC 1 cut(s) 383
HinfI GANTC 2 cut(s) 176, 210
HpaII CCGG 4 cut(s) 154, 186, 342, 366
Hpy166II GTNNAC 2 cut(s) 313, 409
Hpy188I TCNGA 1 cut(s) 167
Hpy188III TCNNGA 3 cut(s) 149, 253, 332
Hpy8I GTNNAC 2 cut(s) 313, 409
HpyAV CCTTC 2 cut(s) 190, 224
Hsp92II CATG 4 cut(s) 110, 116, 226, 417
HspAI GCGC 1 cut(s) 383
KroI GCCGGC 1 cut(s) 365
KroNI GCCGGC 1 cut(s) 367
Kzo9I GATC 2 cut(s) 137, 162
LmnI GCTCC 4 cut(s) 39, 62, 135, 198
LpnPI CCDG 7 cut(s) 167, 199, 266, 334, 344, 355, 379
MaeI CTAG 1 cut(s) 338
MalI GATC 2 cut(s) 139, 164
MboI GATC 2 cut(s) 137, 162
MlsI TGGCCA 3 cut(s) 111, 299, 352
MluCI AATT 1 cut(s) 89
MluNI TGGCCA 3 cut(s) 111, 299, 352
MnlI CCTC 2 cut(s) 156, 411
Mox20I TGGCCA 3 cut(s) 111, 299, 352
MreI CGCCGGCG 1 cut(s) 365
MroNI GCCGGC 1 cut(s) 365
MscI TGGCCA 3 cut(s) 111, 299, 352
MslI CAYNNNNRTG 2 cut(s) 111, 293
Msp20I TGGCCA 3 cut(s) 111, 299, 352
MspI CCGG 4 cut(s) 154, 186, 342, 366
NaeI GCCGGC 1 cut(s) 367
NcoI CCATGG 1 cut(s) 106
NdeII GATC 2 cut(s) 137, 162
NgoMIV GCCGGC 1 cut(s) 365
NlaIII CATG 4 cut(s) 110, 116, 226, 417
PdiI GCCGGC 1 cut(s) 367
PfeI GAWTC 2 cut(s) 176, 210
RsaI GTAC 1 cut(s) 410
RsaNI GTAC 1 cut(s) 409
RseI CAYNNNNRTG 2 cut(s) 111, 293
Sau3AI GATC 2 cut(s) 137, 162
SetI ASST 6 cut(s) 67, 132, 148, 260, 339, 363
SgrAI CRCCGGYG 1 cut(s) 365
SmiMI CAYNNNNRTG 2 cut(s) 111, 293
Sse9I AATT 1 cut(s) 89
SsiI CCGC 1 cut(s) 171
SspMI CTAG 1 cut(s) 338
StyI CCWWGG 3 cut(s) 106, 133, 267
TasI AATT 1 cut(s) 89
TatI WGTACW 1 cut(s) 408
TfiI GAWTC 2 cut(s) 176, 210
TspDTI ATGAA 3 cut(s) 91, 102, 211
XapI RAATTY 1 cut(s) 89
XspI CTAG 1 cut(s) 338
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.