MD10G1293900.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0003774
Physical Location & Seq
Forward (+)
38211878 .. 38212324
447 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1293900.v1.1.491

Sequence Viewer

Length: 447 bp
ATGTCATCGCCGGCGCCGTCGTCGGCCTTCTTCGTCATCTGCCTCCTCCACTCCCTAATCGCCATATCCTGCGGAGCCCTCATGATGTTCTACATGACGGAGGTCTACACCTTCGGGCACGGGACCGAGACCGCCCAGAAGCTGATGGGGTCCACCCCCCACGACCAGCTCCTGATTCGGACCTGCGACTCCTTCTCCGGCCTCCTCCTCTTCGCGATCGGCTTCCTGCTGCTCATGGTCTCCTTCGTGAAGGATCGCGAGTTCCAGACCTTCTTCGCCAAGGGGTGCACGGTGCTTCACGTTTTCATGGCTCTGTGGCGGTGCTACTTCGAGCGGCGGGTGGAGGACCTCGCCTGGGACTGGCTGAGGCAGACGGTCGGCGACGTCTTGCTGGCTGCGTCTTGGGTTTTCTTCCTTGTTTTCTCCTGGAGAGAAAAGTACGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

149

Amino Acids

17.04

Weight (kDa)

6.04

Isoelectric Point (pI)

46.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7865 PF25266 7 - 136 1.4e-69 Domain of unknown function (DUF7865)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 387
Acc36I ACCTGC 1 cut(s) 191
AccB1I GGYRCC 1 cut(s) 13
AccBSI CCGCTC 1 cut(s) 334
AccI GTMKAC 1 cut(s) 105
AccII CGCG 2 cut(s) 215, 258
AciI CCGC 5 cut(s) 72, 132, 319, 334, 337
AclWI GGATC 1 cut(s) 261
AcyI GRCGYC 2 cut(s) 14, 384
AfaI GTAC 1 cut(s) 440
AfiI CCNNNNNNNGG 2 cut(s) 355, 360
AjnI CCWGG 2 cut(s) 353, 425
AloI GAACNNNNNNTCC 2 cut(s) 245, 277
AluBI AGCT 2 cut(s) 142, 169
AluI AGCT 2 cut(s) 142, 169
Alw21I GWGCWC 1 cut(s) 290
Alw26I GTCTC 2 cut(s) 122, 244
Alw44I GTGCAC 1 cut(s) 286
AlwI GGATC 1 cut(s) 261
AlwNI CAGNNNCTG 2 cut(s) 142, 172
AoxI GGCC 2 cut(s) 24, 199
ApaLI GTGCAC 1 cut(s) 286
ApeKI GCWGC 2 cut(s) 229, 395
AspLEI GCGC 1 cut(s) 16
AspS9I GGNCC 4 cut(s) 123, 150, 180, 346
AvaII GGWCC 4 cut(s) 123, 150, 180, 346
BaeGI GKGCMC 2 cut(s) 120, 290
BanI GGYRCC 1 cut(s) 13
BanII GRGCYC 1 cut(s) 79
Bbv12I GWGCWC 1 cut(s) 290
BbvCI CCTCAGC 1 cut(s) 365
BbvI GCAGC 2 cut(s) 216, 382
BccI CCATC 1 cut(s) 139
BciT130I CCWGG 2 cut(s) 355, 427
BcoDI GTCTC 2 cut(s) 122, 244
BfoI RGCGCY 1 cut(s) 17
BfuAI ACCTGC 1 cut(s) 191
BisI GCNGC 3 cut(s) 230, 335, 396
BlsI GCNGC 3 cut(s) 231, 336, 397
Bme1390I CCNGG 2 cut(s) 355, 427
Bme18I GGWCC 4 cut(s) 123, 150, 180, 346
BmgT120I GGNCC 4 cut(s) 123, 150, 180, 346
BmiI GGNNCC 4 cut(s) 15, 76, 124, 151
BmrFI CCNGG 2 cut(s) 355, 427
BoxI GACNNNNGTC 1 cut(s) 101
Bpu10I CCTNAGC 1 cut(s) 365
BsaHI GRCGYC 2 cut(s) 14, 384
BsaI GGTCTC 2 cut(s) 122, 244
BsaJI CCNNGG 2 cut(s) 279, 354
BsaXI ACNNNNNCTCC 2 cut(s) 179, 209
Bsc4I CCNNNNNNNGG 2 cut(s) 355, 360
Bse118I RCCGGY 1 cut(s) 10
Bse1I ACTGG 1 cut(s) 365
BseBI CCWGG 2 cut(s) 355, 427
BseDI CCNNGG 2 cut(s) 279, 354
BseLI CCNNNNNNNGG 2 cut(s) 355, 360
BseMII CTCAG 1 cut(s) 356
BseNI ACTGG 1 cut(s) 365
BseRI GAGGAG 3 cut(s) 35, 194, 197
BseSI GKGCMC 2 cut(s) 120, 290
BseXI GCAGC 2 cut(s) 216, 382
Bsh1236I CGCG 2 cut(s) 215, 258
Bsh1285I CGRYCG 2 cut(s) 219, 378
BshFI GGCC 2 cut(s) 26, 201
BshNI GGYRCC 1 cut(s) 13
BsiEI CGRYCG 2 cut(s) 219, 378
BsiHKAI GWGCWC 1 cut(s) 290
BsiSI CCGG 2 cut(s) 11, 198
BslFI GGGAC 2 cut(s) 136, 371
BslI CCNNNNNNNGG 2 cut(s) 355, 360
BsmAI GTCTC 2 cut(s) 122, 244
BsmFI GGGAC 2 cut(s) 136, 371
BsnI GGCC 2 cut(s) 26, 201
Bso31I GGTCTC 2 cut(s) 122, 244
Bsp1286I GDGCHC 3 cut(s) 79, 120, 290
Bsp143I GATC 2 cut(s) 216, 253
Bsp68I TCGCGA 2 cut(s) 215, 258
BspACI CCGC 5 cut(s) 72, 132, 319, 334, 337
BspANI GGCC 2 cut(s) 26, 201
BspCNI CTCAG 1 cut(s) 357
BspFNI CGCG 2 cut(s) 215, 258
BspHI TCATGA 1 cut(s) 81
BspLI GGNNCC 4 cut(s) 15, 76, 124, 151
BspMI ACCTGC 1 cut(s) 191
BspPI GGATC 1 cut(s) 261
BspT107I GGYRCC 1 cut(s) 13
BspTNI GGTCTC 2 cut(s) 122, 244
BsrBI CCGCTC 1 cut(s) 334
BsrFI RCCGGY 1 cut(s) 10
BsrI ACTGG 1 cut(s) 365
BssAI RCCGGY 1 cut(s) 10
BssECI CCNNGG 2 cut(s) 279, 354
BssMI GATC 2 cut(s) 216, 253
BssNI GRCGYC 2 cut(s) 14, 384
BssT1I CCWWGG 1 cut(s) 279
Bst2UI CCWGG 2 cut(s) 355, 427
Bst4CI ACNGT 2 cut(s) 292, 376
Bst6I CTCTTC 1 cut(s) 215
BstACI GRCGYC 2 cut(s) 14, 384
BstC8I GCNNGC 2 cut(s) 12, 393
BstDEI CTNAG 1 cut(s) 365
BstFNI CGCG 2 cut(s) 215, 258
BstH2I RGCGCY 1 cut(s) 17
BstHHI GCGC 1 cut(s) 16
BstKTI GATC 2 cut(s) 219, 256
BstMAI GTCTC 2 cut(s) 122, 244
BstMBI GATC 2 cut(s) 216, 253
BstMCI CGRYCG 2 cut(s) 219, 378
BstNI CCWGG 2 cut(s) 355, 427
BstPAI GACNNNNGTC 1 cut(s) 101
BstSCI CCNGG 2 cut(s) 353, 425
BstSLI GKGCMC 2 cut(s) 120, 290
BstUI CGCG 2 cut(s) 215, 258
BstV1I GCAGC 2 cut(s) 216, 382
BsuRI GGCC 2 cut(s) 26, 201
BtuMI TCGCGA 2 cut(s) 215, 258
BveI ACCTGC 1 cut(s) 191
Cac8I GCNNGC 2 cut(s) 12, 393
CaiI CAGNNNCTG 2 cut(s) 142, 172
CciI TCATGA 1 cut(s) 81
CfoI GCGC 1 cut(s) 16
Cfr10I RCCGGY 1 cut(s) 10
Cfr13I GGNCC 4 cut(s) 123, 150, 180, 346
CseI GACGC 1 cut(s) 387
Csp6I GTAC 1 cut(s) 439
CviAII CATG 4 cut(s) 82, 94, 235, 307
CviJI RGCY 9 cut(s) 26, 77, 142, 169, 201, 222, 311, 364, 395
CviKI_1 RGCY 9 cut(s) 26, 77, 142, 169, 201, 222, 311, 364, 395
CviQI GTAC 1 cut(s) 439
DdeI CTNAG 1 cut(s) 365
DinI GGCGCC 1 cut(s) 15
DpnI GATC 2 cut(s) 218, 255
DpnII GATC 2 cut(s) 216, 253
Eam1104I CTCTTC 1 cut(s) 215
EarI CTCTTC 1 cut(s) 215
Eco130I CCWWGG 1 cut(s) 279
Eco24I GRGCYC 1 cut(s) 79
Eco31I GGTCTC 2 cut(s) 122, 244
Eco47I GGWCC 4 cut(s) 123, 150, 180, 346
EcoO109I RGGNCCY 1 cut(s) 346
EcoRII CCWGG 2 cut(s) 353, 425
EcoT14I CCWWGG 1 cut(s) 279
EcoT38I GRGCYC 1 cut(s) 79
EgeI GGCGCC 1 cut(s) 15
EheI GGCGCC 1 cut(s) 15
ErhI CCWWGG 1 cut(s) 279
FaeI CATG 4 cut(s) 85, 97, 238, 310
FaiI YATR 5 cut(s) 65, 83, 95, 236, 308
FaqI GGGAC 2 cut(s) 136, 371
FatI CATG 4 cut(s) 81, 93, 234, 306
FauI CCCGC 1 cut(s) 330
FblI GTMKAC 1 cut(s) 105
Fnu4HI GCNGC 3 cut(s) 230, 335, 396
FriOI GRGCYC 1 cut(s) 79
Fsp4HI GCNGC 3 cut(s) 230, 335, 396
GlaI GCGC 1 cut(s) 15
GluI GCNGC 3 cut(s) 230, 335, 396
HaeII RGCGCY 1 cut(s) 17
HaeIII GGCC 2 cut(s) 26, 201
HapII CCGG 2 cut(s) 11, 198
HgaI GACGC 1 cut(s) 387
HhaI GCGC 1 cut(s) 16
Hin1I GRCGYC 2 cut(s) 14, 384
Hin1II CATG 4 cut(s) 85, 97, 238, 310
Hin6I GCGC 1 cut(s) 14
HinP1I GCGC 1 cut(s) 14
HinfI GANTC 2 cut(s) 175, 188
HpaII CCGG 2 cut(s) 11, 198
Hpy166II GTNNAC 3 cut(s) 106, 153, 288
Hpy188I TCNGA 1 cut(s) 180
Hpy188III TCNNGA 6 cut(s) 82, 172, 214, 247, 257, 265
Hpy8I GTNNAC 3 cut(s) 106, 153, 288
Hpy99I CGWCG 3 cut(s) 22, 25, 386
HpyAV CCTTC 6 cut(s) 37, 121, 202, 244, 253, 280
HpyCH4III ACNGT 2 cut(s) 292, 376
HpyCH4IV ACGT 2 cut(s) 300, 384
HpyCH4V TGCA 1 cut(s) 288
HpyF3I CTNAG 1 cut(s) 365
HpySE526I ACGT 2 cut(s) 300, 384
Hsp92I GRCGYC 2 cut(s) 14, 384
Hsp92II CATG 4 cut(s) 85, 97, 238, 310
HspAI GCGC 1 cut(s) 14
KasI GGCGCC 1 cut(s) 13
KroI GCCGGC 1 cut(s) 10
KroNI GCCGGC 1 cut(s) 12
Kzo9I GATC 2 cut(s) 216, 253
LmnI GCTCC 2 cut(s) 74, 174
Lsp1109I GCAGC 2 cut(s) 216, 382
MaeII ACGT 2 cut(s) 300, 384
MalI GATC 2 cut(s) 218, 255
MbiI CCGCTC 1 cut(s) 334
MboI GATC 2 cut(s) 216, 253
MboII GAAGA 4 cut(s) 22, 202, 265, 403
MhlI GDGCHC 3 cut(s) 79, 120, 290
Mly113I GGCGCC 1 cut(s) 14
MlyI GAGTC 1 cut(s) 182
MreI CGCCGGCG 1 cut(s) 10
MroNI GCCGGC 1 cut(s) 10
MspI CCGG 2 cut(s) 11, 198
MspR9I CCNGG 2 cut(s) 355, 427
MvaI CCWGG 2 cut(s) 355, 427
MvnI CGCG 2 cut(s) 215, 258
NaeI GCCGGC 1 cut(s) 12
NarI GGCGCC 1 cut(s) 14
NdeII GATC 2 cut(s) 216, 253
NgoMIV GCCGGC 1 cut(s) 10
NlaIII CATG 4 cut(s) 85, 97, 238, 310
NlaIV GGNNCC 4 cut(s) 15, 76, 124, 151
NruI TCGCGA 2 cut(s) 215, 258
PagI TCATGA 1 cut(s) 81
PcsI WCGNNNNNNNCGW 1 cut(s) 14
PdiI GCCGGC 1 cut(s) 12
PfeI GAWTC 1 cut(s) 175
PfoI TCCNGGA 1 cut(s) 425
PkrI GCNGC 3 cut(s) 231, 336, 397
Ple19I CGATCG 1 cut(s) 219
PleI GAGTC 1 cut(s) 182
PluTI GGCGCC 1 cut(s) 17
PpsI GAGTC 1 cut(s) 182
PpuMI RGGWCCY 1 cut(s) 346
PshAI GACNNNNGTC 1 cut(s) 101
Psp5II RGGWCCY 1 cut(s) 346
Psp6I CCWGG 2 cut(s) 353, 425
PspGI CCWGG 2 cut(s) 353, 425
PspN4I GGNNCC 4 cut(s) 15, 76, 124, 151
PspPI GGNCC 4 cut(s) 123, 150, 180, 346
PspPPI RGGWCCY 1 cut(s) 346
PstNI CAGNNNCTG 2 cut(s) 142, 172
PvuI CGATCG 1 cut(s) 219
RruI TCGCGA 2 cut(s) 215, 258
RsaI GTAC 1 cut(s) 440
RsaNI GTAC 1 cut(s) 439
SatI GCNGC 3 cut(s) 230, 335, 396
Sau3AI GATC 2 cut(s) 216, 253
Sau96I GGNCC 4 cut(s) 123, 150, 180, 346
SchI GAGTC 1 cut(s) 182
ScrFI CCNGG 2 cut(s) 355, 427
SduI GDGCHC 3 cut(s) 79, 120, 290
SetI ASST 9 cut(s) 105, 113, 144, 171, 185, 272, 303, 351, 387
SfoI GGCGCC 1 cut(s) 15
SgrAI CRCCGGYG 1 cut(s) 10
SinI GGWCC 4 cut(s) 123, 150, 180, 346
SsiI CCGC 5 cut(s) 72, 132, 319, 334, 337
SspDI GGCGCC 1 cut(s) 13
StyD4I CCNGG 2 cut(s) 353, 425
StyI CCWWGG 1 cut(s) 279
TaaI ACNGT 2 cut(s) 292, 376
TaiI ACGT 2 cut(s) 303, 387
TaqI TCGA 1 cut(s) 330
TaqII GACCGA 1 cut(s) 140
TauI GCSGC 1 cut(s) 337
TfiI GAWTC 1 cut(s) 175
TseI GCWGC 2 cut(s) 229, 395
TspDTI ATGAA 1 cut(s) 295
TspGWI ACGGA 1 cut(s) 113
VneI GTGCAC 1 cut(s) 286
VpaK11BI GGWCC 4 cut(s) 123, 150, 180, 346
XmiI GTMKAC 1 cut(s) 105
ZraI GACGTC 1 cut(s) 385
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.