MD11G1247000.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0003774
Physical Location & Seq
Reverse (-)
35888036 .. 35888470
435 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1247000.v1.1.491

Sequence Viewer

Length: 435 bp
ATGGGGTTATCTGGGTTTTTTCTAATATGCATGCTCCACTCTGTGATTGCTCTGATTTGTGGAGCTTTCATGATGTTCTACTCCTACGAATTCTCTGTGTTCAGCCATGGCCATGAGACAGCAAGCAAGCTCCAAGGGTCGACGCCTCACGACCGGCTTTTGATCCGAACAGCGGATTCCTTCTCCGGATTCCTTCTGTTTGCCATTGGATTCCTTCTGTTCATGGTTGCTTTTGTCAAAGACCATAAGTTCCAGAGCTTCTTTGCCAAGGGTTGCGTGTTGCTCCACATTTCCATGGCCATTTGGAGAGTTTACTTCGAGAGGAACCTCGAAGAGCTCGCCGGAGACTGGCCAAAACATGTCGCCGGTGACGTCACATTGGCGCTTTCATGGGTGTTACTTCTTGTGTACTCATGGAGGGAGAAGTATGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

145

Amino Acids

16.51

Weight (kDa)

6.64

Isoelectric Point (pI)

24.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7865 PF25266 1 - 132 3.9e-67 Domain of unknown function (DUF7865)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 375
AccI GTMKAC 1 cut(s) 140
AccIII TCCGGA 1 cut(s) 185
AciI CCGC 1 cut(s) 173
AclWI GGATC 1 cut(s) 157
AcoI YGGCCR 3 cut(s) 109, 297, 350
AcsI RAATTY 1 cut(s) 89
AcyI GRCGYC 2 cut(s) 143, 372
AdeI CACNNNGTG 1 cut(s) 43
AfaI GTAC 1 cut(s) 410
AfiI CCNNNNNNNGG 2 cut(s) 172, 348
AflIII ACRYGT 1 cut(s) 358
AluBI AGCT 4 cut(s) 65, 130, 258, 337
AluI AGCT 4 cut(s) 65, 130, 258, 337
Alw21I GWGCWC 1 cut(s) 339
Alw26I GTCTC 2 cut(s) 110, 339
AlwI GGATC 1 cut(s) 157
Aor13HI TCCGGA 1 cut(s) 185
AoxI GGCC 3 cut(s) 109, 297, 350
ApoI RAATTY 1 cut(s) 89
AspLEI GCGC 1 cut(s) 385
AsuHPI GGTGA 1 cut(s) 380
BalI TGGCCA 3 cut(s) 111, 299, 352
BanII GRGCYC 1 cut(s) 339
Bbv12I GWGCWC 1 cut(s) 339
BcoDI GTCTC 2 cut(s) 110, 339
BfoI RGCGCY 1 cut(s) 386
BmiI GGNNCC 1 cut(s) 326
BsaHI GRCGYC 2 cut(s) 143, 372
BsaJI CCNNGG 4 cut(s) 106, 133, 267, 294
BsaWI WCCGGW 1 cut(s) 185
Bsc4I CCNNNNNNNGG 2 cut(s) 172, 348
Bse118I RCCGGY 2 cut(s) 153, 365
Bse1I ACTGG 1 cut(s) 353
BseAI TCCGGA 1 cut(s) 185
BseDI CCNNGG 4 cut(s) 106, 133, 267, 294
BseLI CCNNNNNNNGG 2 cut(s) 172, 348
BseNI ACTGG 1 cut(s) 353
Bsh1285I CGRYCG 1 cut(s) 154
BshFI GGCC 3 cut(s) 111, 299, 352
BsiEI CGRYCG 1 cut(s) 154
BsiHKAI GWGCWC 1 cut(s) 339
BsiSI CCGG 4 cut(s) 154, 186, 342, 366
BslI CCNNNNNNNGG 2 cut(s) 172, 348
BsmAI GTCTC 2 cut(s) 110, 339
BsnI GGCC 3 cut(s) 111, 299, 352
Bsp1286I GDGCHC 1 cut(s) 339
Bsp13I TCCGGA 1 cut(s) 185
Bsp143I GATC 1 cut(s) 162
Bsp19I CCATGG 2 cut(s) 106, 294
BspACI CCGC 1 cut(s) 173
BspANI GGCC 3 cut(s) 111, 299, 352
BspEI TCCGGA 1 cut(s) 185
BspHI TCATGA 1 cut(s) 69
BspLI GGNNCC 1 cut(s) 326
BspPI GGATC 1 cut(s) 157
BspQI GCTCTTC 1 cut(s) 327
BsrFI RCCGGY 2 cut(s) 153, 365
BsrI ACTGG 1 cut(s) 353
BssAI RCCGGY 2 cut(s) 153, 365
BssECI CCNNGG 4 cut(s) 106, 133, 267, 294
BssMI GATC 1 cut(s) 162
BssNI GRCGYC 2 cut(s) 143, 372
BssT1I CCWWGG 4 cut(s) 106, 133, 267, 294
Bst6I CTCTTC 1 cut(s) 327
BstACI GRCGYC 2 cut(s) 143, 372
BstC8I GCNNGC 4 cut(s) 32, 124, 128, 339
BstDSI CCRYGG 2 cut(s) 106, 294
BstH2I RGCGCY 1 cut(s) 386
BstHHI GCGC 1 cut(s) 385
BstKTI GATC 1 cut(s) 165
BstMAI GTCTC 2 cut(s) 110, 339
BstMBI GATC 1 cut(s) 162
BstMCI CGRYCG 1 cut(s) 154
BstNSI RCATGY 2 cut(s) 34, 362
BsuRI GGCC 3 cut(s) 111, 299, 352
BtgI CCRYGG 2 cut(s) 106, 294
Cac8I GCNNGC 4 cut(s) 32, 124, 128, 339
CciI TCATGA 1 cut(s) 69
CfoI GCGC 1 cut(s) 385
Cfr10I RCCGGY 2 cut(s) 153, 365
CseI GACGC 1 cut(s) 151
Csp6I GTAC 1 cut(s) 409
CviAII CATG 9 cut(s) 31, 70, 107, 113, 223, 295, 359, 390, 414
CviJI RGCY 9 cut(s) 65, 105, 111, 130, 157, 258, 299, 337, 352
CviKI_1 RGCY 9 cut(s) 65, 105, 111, 130, 157, 258, 299, 337, 352
CviQI GTAC 1 cut(s) 409
DpnI GATC 1 cut(s) 164
DpnII GATC 1 cut(s) 162
DraIII CACNNNGTG 1 cut(s) 43
EaeI YGGCCR 3 cut(s) 109, 297, 350
Eam1104I CTCTTC 1 cut(s) 327
EarI CTCTTC 1 cut(s) 327
Ecl136II GAGCTC 1 cut(s) 337
Eco130I CCWWGG 4 cut(s) 106, 133, 267, 294
Eco24I GRGCYC 1 cut(s) 339
Eco53kI GAGCTC 1 cut(s) 337
EcoICRI GAGCTC 1 cut(s) 337
EcoRI GAATTC 1 cut(s) 89
EcoT14I CCWWGG 4 cut(s) 106, 133, 267, 294
EcoT22I ATGCAT 1 cut(s) 32
EcoT38I GRGCYC 1 cut(s) 339
ErhI CCWWGG 4 cut(s) 106, 133, 267, 294
FaeI CATG 9 cut(s) 34, 73, 110, 116, 226, 298, 362, 393, 417
FatI CATG 9 cut(s) 30, 69, 106, 112, 222, 294, 358, 389, 413
FblI GTMKAC 1 cut(s) 140
FriOI GRGCYC 1 cut(s) 339
GlaI GCGC 1 cut(s) 384
HaeII RGCGCY 1 cut(s) 386
HaeIII GGCC 3 cut(s) 111, 299, 352
HapII CCGG 4 cut(s) 154, 186, 342, 366
HgaI GACGC 1 cut(s) 151
HhaI GCGC 1 cut(s) 385
Hin1I GRCGYC 2 cut(s) 143, 372
Hin1II CATG 9 cut(s) 34, 73, 110, 116, 226, 298, 362, 393, 417
Hin6I GCGC 1 cut(s) 383
HinP1I GCGC 1 cut(s) 383
HincII GTYRAC 1 cut(s) 141
HindII GTYRAC 1 cut(s) 141
HinfI GANTC 3 cut(s) 176, 189, 210
HpaII CCGG 4 cut(s) 154, 186, 342, 366
HphI GGTGA 1 cut(s) 380
Hpy166II GTNNAC 3 cut(s) 141, 313, 409
Hpy188I TCNGA 2 cut(s) 54, 167
Hpy188III TCNNGA 5 cut(s) 70, 149, 186, 253, 319
Hpy8I GTNNAC 3 cut(s) 141, 313, 409
Hpy99I CGWCG 1 cut(s) 145
HpyAV CCTTC 3 cut(s) 190, 203, 224
HpyCH4IV ACGT 1 cut(s) 372
HpyCH4V TGCA 1 cut(s) 30
HpySE526I ACGT 1 cut(s) 372
Hsp92I GRCGYC 2 cut(s) 143, 372
Hsp92II CATG 9 cut(s) 34, 73, 110, 116, 226, 298, 362, 393, 417
HspAI GCGC 1 cut(s) 383
Kpn2I TCCGGA 1 cut(s) 185
Kzo9I GATC 1 cut(s) 162
LguI GCTCTTC 1 cut(s) 327
LmnI GCTCC 4 cut(s) 39, 62, 135, 288
LpnPI CCDG 6 cut(s) 167, 199, 266, 334, 355, 379
MaeII ACGT 1 cut(s) 372
MaeIII GTNAC 3 cut(s) 368, 373, 396
MalI GATC 1 cut(s) 164
MboI GATC 1 cut(s) 162
MboII GAAGA 1 cut(s) 344
MhlI GDGCHC 1 cut(s) 339
MlsI TGGCCA 3 cut(s) 111, 299, 352
MluCI AATT 1 cut(s) 89
MluNI TGGCCA 3 cut(s) 111, 299, 352
MnlI CCTC 4 cut(s) 156, 315, 338, 411
Mox20I TGGCCA 3 cut(s) 111, 299, 352
Mph1103I ATGCAT 1 cut(s) 32
MroI TCCGGA 1 cut(s) 185
MscI TGGCCA 3 cut(s) 111, 299, 352
MslI CAYNNNNRTG 2 cut(s) 111, 293
Msp20I TGGCCA 3 cut(s) 111, 299, 352
MspA1I CMGCKG 1 cut(s) 173
MspI CCGG 4 cut(s) 154, 186, 342, 366
NcoI CCATGG 2 cut(s) 106, 294
NdeII GATC 1 cut(s) 162
NlaIII CATG 9 cut(s) 34, 73, 110, 116, 226, 298, 362, 393, 417
NlaIV GGNNCC 1 cut(s) 326
NmuCI GTSAC 2 cut(s) 368, 373
NsiI ATGCAT 1 cut(s) 32
NspI RCATGY 2 cut(s) 34, 362
PaeI GCATGC 1 cut(s) 34
PagI TCATGA 1 cut(s) 69
PciI ACATGT 1 cut(s) 358
PciSI GCTCTTC 1 cut(s) 327
PcsI WCGNNNNNNNCGW 1 cut(s) 369
PfeI GAWTC 3 cut(s) 176, 189, 210
PscI ACATGT 1 cut(s) 358
Psp124BI GAGCTC 1 cut(s) 339
PspN4I GGNNCC 1 cut(s) 326
RsaI GTAC 1 cut(s) 410
RsaNI GTAC 1 cut(s) 409
RseI CAYNNNNRTG 2 cut(s) 111, 293
SacI GAGCTC 1 cut(s) 339
SalI GTCGAC 1 cut(s) 139
SapI GCTCTTC 1 cut(s) 327
Sau3AI GATC 1 cut(s) 162
SduI GDGCHC 1 cut(s) 339
SetI ASST 6 cut(s) 67, 132, 260, 330, 339, 375
SgrAI CRCCGGYG 1 cut(s) 365
SmiMI CAYNNNNRTG 2 cut(s) 111, 293
SphI GCATGC 1 cut(s) 34
Sse9I AATT 1 cut(s) 89
SsiI CCGC 1 cut(s) 173
SstI GAGCTC 1 cut(s) 339
StyI CCWWGG 4 cut(s) 106, 133, 267, 294
TaiI ACGT 1 cut(s) 375
TaqI TCGA 3 cut(s) 140, 318, 330
TasI AATT 1 cut(s) 89
TatI WGTACW 1 cut(s) 408
TfiI GAWTC 3 cut(s) 176, 189, 210
TseFI GTSAC 2 cut(s) 368, 373
Tsp45I GTSAC 2 cut(s) 368, 373
TspDTI ATGAA 3 cut(s) 58, 211, 378
XapI RAATTY 1 cut(s) 89
XceI RCATGY 2 cut(s) 34, 362
XmiI GTMKAC 1 cut(s) 140
ZraI GACGTC 1 cut(s) 373
Zsp2I ATGCAT 1 cut(s) 32
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.