FvH4_3g09111

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0003774
Physical Location & Seq
Forward (+)
5342148 .. 5342603
456 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g09111.t1

Sequence Viewer

Length: 456 bp
ATGCCACCGCCGTCGTCGCCGTCGACGTCATGGCCGTTCACCGTGATCTGCCTCCTCCACTCGGCGATCGCGATCGCGTGCGGCGCTTGCATGATGTTCTACATGACGGATCTCTACACCTTCGGCCACGGCGTCGAGACGGCGCAGAAGCTCCTCGGCTCGACGCCGCACGACCAGCTCCTGATCCGGACCTCCGACTCGTTCTCCGGCTTGCTCCTCTTCGCGATCGGCTTCCTTCTCCTCATGGTCTCCTTCGTCAGAGATCGCGAGTTCCAGAGCTTCTTCGCCAAGGGCTGCACGGTCCTCCATGTCTTCATGGGACTCTGGCGGTGCTACTTCGAGCGGAGAGTCGAGGATCTCGCCTGGGATTGGCTCCGCCAGACAGTCGGCGACGTCGTTTTGGCCCTCTCTTGGGTTTTCTTCCTCGTGTACTCGTGGAGAGAAAAGTACGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

152

Amino Acids

17.37

Weight (kDa)

6.04

Isoelectric Point (pI)

51.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7865 PF25266 11 - 139 7.5e-68 Domain of unknown function (DUF7865)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 29, 396
AccBSI CCGCTC 1 cut(s) 343
AccI GTMKAC 1 cut(s) 23
AccII CGCG 4 cut(s) 71, 77, 224, 267
AccIII TCCGGA 1 cut(s) 186
AciI CCGC 6 cut(s) 8, 81, 167, 328, 343, 376
AclWI GGATC 3 cut(s) 117, 178, 363
AcoI YGGCCR 2 cut(s) 32, 124
AcyI GRCGYC 4 cut(s) 26, 132, 164, 393
AfaI GTAC 2 cut(s) 431, 449
AfiI CCNNNNNNNGG 4 cut(s) 61, 369, 411, 412
AjnI CCWGG 1 cut(s) 362
AluBI AGCT 3 cut(s) 151, 178, 279
AluI AGCT 3 cut(s) 151, 178, 279
Alw26I GTCTC 2 cut(s) 131, 253
AlwI GGATC 3 cut(s) 117, 178, 363
AlwNI CAGNNNCTG 1 cut(s) 181
Aor13HI TCCGGA 1 cut(s) 186
AoxI GGCC 3 cut(s) 32, 124, 402
ApeKI GCWGC 1 cut(s) 294
AsiSI GCGATCGC 2 cut(s) 69, 75
AspLEI GCGC 2 cut(s) 86, 145
AspS9I GGNCC 3 cut(s) 189, 301, 403
AsuHPI GGTGA 1 cut(s) 31
AvaII GGWCC 2 cut(s) 189, 301
BauI CACGAG 2 cut(s) 425, 433
BbsI GAAGAC 1 cut(s) 304
BbvI GCAGC 1 cut(s) 281
BceAI ACGGC 4 cut(s) 4, 19, 145, 156
BciT130I CCWGG 1 cut(s) 364
BcoDI GTCTC 2 cut(s) 131, 253
BfoI RGCGCY 1 cut(s) 87
BisI GCNGC 3 cut(s) 82, 167, 295
BlsI GCNGC 3 cut(s) 83, 168, 296
Bme1390I CCNGG 1 cut(s) 364
Bme18I GGWCC 2 cut(s) 189, 301
BmgT120I GGNCC 3 cut(s) 189, 301, 403
BmiI GGNNCC 1 cut(s) 374
BmrFI CCNGG 1 cut(s) 364
BpiI GAAGAC 1 cut(s) 304
BsaBI GATNNNNATC 1 cut(s) 71
BsaHI GRCGYC 4 cut(s) 26, 132, 164, 393
BsaI GGTCTC 1 cut(s) 253
BsaJI CCNNGG 4 cut(s) 127, 154, 288, 363
BsaWI WCCGGW 1 cut(s) 186
BsaXI ACNNNNNCTCC 2 cut(s) 188, 218
Bsc4I CCNNNNNNNGG 4 cut(s) 61, 369, 411, 412
Bse8I GATNNNNATC 1 cut(s) 71
BseAI TCCGGA 1 cut(s) 186
BseBI CCWGG 1 cut(s) 364
BseDI CCNNGG 4 cut(s) 127, 154, 288, 363
BseJI GATNNNNATC 1 cut(s) 71
BseLI CCNNNNNNNGG 4 cut(s) 61, 369, 411, 412
BseRI GAGGAG 4 cut(s) 44, 143, 206, 230
BseXI GCAGC 1 cut(s) 281
BsgI GTGCAG 1 cut(s) 280
Bsh1236I CGCG 4 cut(s) 71, 77, 224, 267
Bsh1285I CGRYCG 3 cut(s) 69, 75, 228
BshFI GGCC 3 cut(s) 34, 126, 404
BsiEI CGRYCG 3 cut(s) 69, 75, 228
BsiSI CCGG 2 cut(s) 187, 207
BslFI GGGAC 1 cut(s) 333
BslI CCNNNNNNNGG 4 cut(s) 61, 369, 411, 412
BsmAI GTCTC 2 cut(s) 131, 253
BsmBI CGTCTC 1 cut(s) 131
BsmFI GGGAC 1 cut(s) 333
BsnI GGCC 3 cut(s) 34, 126, 404
Bso31I GGTCTC 1 cut(s) 253
Bsp13I TCCGGA 1 cut(s) 186
Bsp143I GATC 8 cut(s) 45, 66, 72, 109, 183, 225, 262, 355
Bsp68I TCGCGA 3 cut(s) 71, 224, 267
BspACI CCGC 6 cut(s) 8, 81, 167, 328, 343, 376
BspANI GGCC 3 cut(s) 34, 126, 404
BspEI TCCGGA 1 cut(s) 186
BspFNI CGCG 4 cut(s) 71, 77, 224, 267
BspLI GGNNCC 1 cut(s) 374
BspPI GGATC 3 cut(s) 117, 178, 363
BspTNI GGTCTC 1 cut(s) 253
BsrBI CCGCTC 1 cut(s) 343
BssECI CCNNGG 4 cut(s) 127, 154, 288, 363
BssMI GATC 8 cut(s) 45, 66, 72, 109, 183, 225, 262, 355
BssNI GRCGYC 4 cut(s) 26, 132, 164, 393
BssSI CACGAG 2 cut(s) 425, 433
BssT1I CCWWGG 1 cut(s) 288
Bst2BI CACGAG 2 cut(s) 425, 433
Bst2UI CCWGG 1 cut(s) 364
Bst4CI ACNGT 3 cut(s) 43, 301, 385
Bst6I CTCTTC 1 cut(s) 224
BstACI GRCGYC 4 cut(s) 26, 132, 164, 393
BstC8I GCNNGC 3 cut(s) 79, 88, 212
BstDSI CCRYGG 1 cut(s) 127
BstFNI CGCG 4 cut(s) 71, 77, 224, 267
BstH2I RGCGCY 1 cut(s) 87
BstHHI GCGC 2 cut(s) 86, 145
BstKTI GATC 8 cut(s) 48, 69, 75, 112, 186, 228, 265, 358
BstMAI GTCTC 2 cut(s) 131, 253
BstMBI GATC 8 cut(s) 45, 66, 72, 109, 183, 225, 262, 355
BstMCI CGRYCG 3 cut(s) 69, 75, 228
BstMWI GCNNNNNNNGC 4 cut(s) 16, 83, 87, 175
BstNI CCWGG 1 cut(s) 364
BstSCI CCNGG 1 cut(s) 362
BstUI CGCG 4 cut(s) 71, 77, 224, 267
BstV1I GCAGC 1 cut(s) 281
BstV2I GAAGAC 1 cut(s) 304
BstX2I RGATCY 2 cut(s) 109, 355
BstYI RGATCY 2 cut(s) 109, 355
BsuRI GGCC 3 cut(s) 34, 126, 404
BtgI CCRYGG 1 cut(s) 127
BtuMI TCGCGA 3 cut(s) 71, 224, 267
Cac8I GCNNGC 3 cut(s) 79, 88, 212
CaiI CAGNNNCTG 1 cut(s) 181
CfoI GCGC 2 cut(s) 86, 145
Cfr13I GGNCC 3 cut(s) 189, 301, 403
CseI GACGC 2 cut(s) 121, 172
Csp6I GTAC 2 cut(s) 430, 448
CviAII CATG 6 cut(s) 30, 91, 103, 244, 308, 316
CviQI GTAC 2 cut(s) 430, 448
DpnI GATC 8 cut(s) 47, 68, 74, 111, 185, 227, 264, 357
DpnII GATC 8 cut(s) 45, 66, 72, 109, 183, 225, 262, 355
EaeI YGGCCR 2 cut(s) 32, 124
Eam1104I CTCTTC 1 cut(s) 224
EarI CTCTTC 1 cut(s) 224
EciI GGCGGA 1 cut(s) 365
Eco130I CCWWGG 1 cut(s) 288
Eco31I GGTCTC 1 cut(s) 253
Eco47I GGWCC 2 cut(s) 189, 301
EcoRII CCWGG 1 cut(s) 362
EcoT14I CCWWGG 1 cut(s) 288
ErhI CCWWGG 1 cut(s) 288
Esp3I CGTCTC 1 cut(s) 131
FaeI CATG 6 cut(s) 33, 94, 106, 247, 311, 319
FaiI YATR 6 cut(s) 31, 92, 104, 245, 309, 317
FaqI GGGAC 1 cut(s) 333
FatI CATG 6 cut(s) 29, 90, 102, 243, 307, 315
FblI GTMKAC 1 cut(s) 23
Fnu4HI GCNGC 3 cut(s) 82, 167, 295
Fsp4HI GCNGC 3 cut(s) 82, 167, 295
GlaI GCGC 2 cut(s) 85, 144
GluI GCNGC 3 cut(s) 82, 167, 295
HaeII RGCGCY 1 cut(s) 87
HaeIII GGCC 3 cut(s) 34, 126, 404
HapII CCGG 2 cut(s) 187, 207
HgaI GACGC 2 cut(s) 121, 172
HhaI GCGC 2 cut(s) 86, 145
Hin1I GRCGYC 4 cut(s) 26, 132, 164, 393
Hin1II CATG 6 cut(s) 33, 94, 106, 247, 311, 319
Hin6I GCGC 2 cut(s) 84, 143
HinP1I GCGC 2 cut(s) 84, 143
HincII GTYRAC 1 cut(s) 24
HindII GTYRAC 1 cut(s) 24
HinfI GANTC 3 cut(s) 197, 321, 348
HpaII CCGG 2 cut(s) 187, 207
HphI GGTGA 1 cut(s) 31
Hpy166II GTNNAC 3 cut(s) 24, 39, 430
Hpy188I TCNGA 2 cut(s) 196, 260
Hpy188III TCNNGA 7 cut(s) 70, 136, 181, 187, 223, 266, 274
Hpy8I GTNNAC 3 cut(s) 24, 39, 430
Hpy99I CGWCG 8 cut(s) 16, 19, 25, 28, 137, 166, 395, 398
HpyAV CCTTC 3 cut(s) 130, 245, 262
HpyCH4III ACNGT 3 cut(s) 43, 301, 385
HpyCH4IV ACGT 2 cut(s) 26, 393
HpyCH4V TGCA 2 cut(s) 90, 297
HpyF10VI GCNNNNNNNGC 4 cut(s) 16, 83, 87, 175
HpySE526I ACGT 2 cut(s) 26, 393
Hsp92I GRCGYC 4 cut(s) 26, 132, 164, 393
Hsp92II CATG 6 cut(s) 33, 94, 106, 247, 311, 319
HspAI GCGC 2 cut(s) 84, 143
Kpn2I TCCGGA 1 cut(s) 186
Kzo9I GATC 8 cut(s) 45, 66, 72, 109, 183, 225, 262, 355
LmnI GCTCC 4 cut(s) 156, 183, 219, 378
LpnPI CCDG 9 cut(s) 188, 194, 200, 220, 287, 310, 349, 376, 392
Lsp1109I GCAGC 1 cut(s) 281
MaeII ACGT 2 cut(s) 26, 393
MalI GATC 8 cut(s) 47, 68, 74, 111, 185, 227, 264, 357
MbiI CCGCTC 1 cut(s) 343
MboI GATC 8 cut(s) 45, 66, 72, 109, 183, 225, 262, 355
MboII GAAGA 4 cut(s) 211, 274, 304, 412
MflI RGATCY 2 cut(s) 109, 355
MlyI GAGTC 3 cut(s) 191, 315, 357
MmeI TCCRAC 1 cut(s) 219
MroI TCCGGA 1 cut(s) 186
MseI TTAA 1 cut(s) 454
MspI CCGG 2 cut(s) 187, 207
MspR9I CCNGG 1 cut(s) 364
MvaI CCWGG 1 cut(s) 364
MvnI CGCG 4 cut(s) 71, 77, 224, 267
MwoI GCNNNNNNNGC 4 cut(s) 16, 83, 87, 175
NdeII GATC 8 cut(s) 45, 66, 72, 109, 183, 225, 262, 355
NlaIII CATG 6 cut(s) 33, 94, 106, 247, 311, 319
NlaIV GGNNCC 1 cut(s) 374
NmeAIII GCCGAG 2 cut(s) 41, 135
NruI TCGCGA 3 cut(s) 71, 224, 267
PcsI WCGNNNNNNNCGW 6 cut(s) 20, 23, 32, 68, 129, 393
PkrI GCNGC 3 cut(s) 83, 168, 296
Ple19I CGATCG 3 cut(s) 69, 75, 228
PleI GAGTC 3 cut(s) 191, 315, 356
PpsI GAGTC 3 cut(s) 191, 315, 356
Psp6I CCWGG 1 cut(s) 362
PspGI CCWGG 1 cut(s) 362
PspN4I GGNNCC 1 cut(s) 374
PspPI GGNCC 3 cut(s) 189, 301, 403
PstNI CAGNNNCTG 1 cut(s) 181
PsuI RGATCY 2 cut(s) 109, 355
PvuI CGATCG 3 cut(s) 69, 75, 228
RgaI GCGATCGC 2 cut(s) 69, 75
RruI TCGCGA 3 cut(s) 71, 224, 267
RsaI GTAC 2 cut(s) 431, 449
RsaNI GTAC 2 cut(s) 430, 448
SalI GTCGAC 1 cut(s) 22
SaqAI TTAA 1 cut(s) 454
SatI GCNGC 3 cut(s) 82, 167, 295
Sau3AI GATC 8 cut(s) 45, 66, 72, 109, 183, 225, 262, 355
Sau96I GGNCC 3 cut(s) 189, 301, 403
SchI GAGTC 3 cut(s) 191, 315, 357
ScrFI CCNGG 1 cut(s) 364
SetI ASST 7 cut(s) 29, 122, 153, 180, 194, 281, 396
SfaAI GCGATCGC 2 cut(s) 69, 75
SgfI GCGATCGC 2 cut(s) 69, 75
SgrDI CGTCGACG 1 cut(s) 22
SinI GGWCC 2 cut(s) 189, 301
SsiI CCGC 6 cut(s) 8, 81, 167, 328, 343, 376
StyD4I CCNGG 1 cut(s) 362
StyI CCWWGG 1 cut(s) 288
TaaI ACNGT 3 cut(s) 43, 301, 385
TaiI ACGT 2 cut(s) 29, 396
TaqI TCGA 5 cut(s) 23, 135, 161, 339, 351
TatI WGTACW 1 cut(s) 429
TauI GCSGC 2 cut(s) 84, 169
Tru1I TTAA 1 cut(s) 454
Tru9I TTAA 1 cut(s) 454
TseI GCWGC 1 cut(s) 294
TspDTI ATGAA 1 cut(s) 304
TspGWI ACGGA 1 cut(s) 122
VpaK11BI GGWCC 2 cut(s) 189, 301
XmiI GTMKAC 1 cut(s) 23
ZraI GACGTC 2 cut(s) 27, 394
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.