RLG00000031577

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0003774
Physical Location & Seq
Reverse (-)
6350087 .. 6351329
1243 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000031577

Sequence Viewer

Length: 504 bp
ATGTCATCGCCGCCGTCGTCGTCGGCGTTCTCCGTGATCTGCCTGCTCCACGCGGCGATCGCCATCGCGTGTGGCGCGTCCATGATGTTCTACATGTCGGATCTGTACACCTTGGGGCACGGAGTCGAGACGGCGCAGAAGCTTCTCGGGTCGACGCCGCACGACCAGCTCCTGATCCGGACCTCCGACTCGTTCTCCGGGTTGCTCCTCTTCGCGATCGGGTTCCTGCTCCTCATGGTCTCCTTCGTCAGAGATCGCGAGTTCCAGAGCTTCTTCGCCAAGGGCTGCACGCTCCTCCATGTCTTCATGGGCCTCTGGCGCTGCTACTTCGAGCGCAGGGTCGAGGATCTCGCCTGGGATTGGCTCCGGCAGACCGTCGGCGACGTCGTTTTGGCCCTCTCTTGGGTCTTCTTCCTTGTGTTCTCGTGGAGAGAAAAGATGAATTATTGCTGCTTGATGAGTGTTTCTCTTATAGGGACTGAGGTCAACTCAGTCTCGAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

168

Amino Acids

18.8

Weight (kDa)

6.03

Isoelectric Point (pI)

49.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7865 PF25266 6 - 136 2.2e-68 Domain of unknown function (DUF7865)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 387
AccI GTMKAC 1 cut(s) 152
AccII CGCG 5 cut(s) 53, 68, 77, 215, 258
AccIII TCCGGA 1 cut(s) 177
AciI CCGC 3 cut(s) 11, 53, 158
AclWI GGATC 3 cut(s) 108, 169, 354
AcyI GRCGYC 2 cut(s) 155, 384
AfaI GTAC 1 cut(s) 107
AfiI CCNNNNNNNGG 3 cut(s) 360, 402, 403
AflIII ACRYGT 1 cut(s) 93
AjnI CCWGG 1 cut(s) 353
AluBI AGCT 3 cut(s) 142, 169, 270
AluI AGCT 3 cut(s) 142, 169, 270
Alw26I GTCTC 3 cut(s) 122, 244, 499
AlwI GGATC 3 cut(s) 108, 169, 354
AlwNI CAGNNNCTG 1 cut(s) 172
Ama87I CYCGRG 2 cut(s) 146, 496
Aor13HI TCCGGA 1 cut(s) 177
AoxI GGCC 2 cut(s) 310, 393
ApeKI GCWGC 3 cut(s) 285, 321, 450
AsiSI GCGATCGC 1 cut(s) 60
AspLEI GCGC 4 cut(s) 77, 136, 321, 336
AspS9I GGNCC 3 cut(s) 180, 310, 394
AsuC2I CCSGG 1 cut(s) 199
AvaI CYCGRG 2 cut(s) 146, 496
AvaII GGWCC 1 cut(s) 180
BaeGI GKGCMC 1 cut(s) 120
BauI CACGAG 1 cut(s) 424
BbsI GAAGAC 2 cut(s) 295, 400
BbvI GCAGC 3 cut(s) 272, 308, 437
BccI CCATC 1 cut(s) 71
BceAI ACGGC 1 cut(s) 147
BciT130I CCWGG 1 cut(s) 355
BcnI CCSGG 1 cut(s) 199
BcoDI GTCTC 3 cut(s) 122, 244, 499
BfoI RGCGCY 1 cut(s) 322
BisI GCNGC 6 cut(s) 11, 54, 158, 286, 322, 451
BlsI GCNGC 6 cut(s) 12, 55, 159, 287, 323, 452
Bme1390I CCNGG 2 cut(s) 199, 355
Bme18I GGWCC 1 cut(s) 180
BmeT110I CYCGRG 2 cut(s) 146, 496
BmgT120I GGNCC 3 cut(s) 180, 310, 394
BmiI GGNNCC 2 cut(s) 224, 365
BmrFI CCNGG 2 cut(s) 199, 355
BoxI GACNNNNGTC 1 cut(s) 482
BpiI GAAGAC 2 cut(s) 295, 400
BplI GAGNNNNNCTC 3 cut(s) 451, 483, 473
BpuMI CCSGG 1 cut(s) 199
BsaBI GATNNNNATC 1 cut(s) 62
BsaHI GRCGYC 2 cut(s) 155, 384
BsaI GGTCTC 1 cut(s) 244
BsaJI CCNNGG 3 cut(s) 111, 279, 354
BsaWI WCCGGW 1 cut(s) 177
BsaXI ACNNNNNCTCC 2 cut(s) 179, 209
Bsc4I CCNNNNNNNGG 3 cut(s) 360, 402, 403
Bse8I GATNNNNATC 1 cut(s) 62
BseAI TCCGGA 1 cut(s) 177
BseBI CCWGG 1 cut(s) 355
BseDI CCNNGG 3 cut(s) 111, 279, 354
BseJI GATNNNNATC 1 cut(s) 62
BseLI CCNNNNNNNGG 3 cut(s) 360, 402, 403
BseMII CTCAG 2 cut(s) 471, 504
BseRI GAGGAG 3 cut(s) 197, 221, 284
BseSI GKGCMC 1 cut(s) 120
BseXI GCAGC 3 cut(s) 272, 308, 437
BsgI GTGCAG 1 cut(s) 271
Bsh1236I CGCG 5 cut(s) 53, 68, 77, 215, 258
Bsh1285I CGRYCG 2 cut(s) 60, 219
BshFI GGCC 2 cut(s) 312, 395
BsiEI CGRYCG 2 cut(s) 60, 219
BsiHKCI CYCGRG 2 cut(s) 146, 496
BsiSI CCGG 3 cut(s) 178, 198, 367
BslFI GGGAC 1 cut(s) 490
BslI CCNNNNNNNGG 3 cut(s) 360, 402, 403
BsmAI GTCTC 3 cut(s) 122, 244, 499
BsmBI CGTCTC 1 cut(s) 122
BsmFI GGGAC 1 cut(s) 490
BsnI GGCC 2 cut(s) 312, 395
Bso31I GGTCTC 1 cut(s) 244
BsoBI CYCGRG 2 cut(s) 146, 496
Bsp1286I GDGCHC 1 cut(s) 120
Bsp13I TCCGGA 1 cut(s) 177
Bsp1407I TGTACA 1 cut(s) 105
Bsp143I GATC 7 cut(s) 36, 57, 100, 174, 216, 253, 346
Bsp68I TCGCGA 2 cut(s) 215, 258
BspACI CCGC 3 cut(s) 11, 53, 158
BspANI GGCC 2 cut(s) 312, 395
BspCNI CTCAG 2 cut(s) 472, 503
BspEI TCCGGA 1 cut(s) 177
BspFNI CGCG 5 cut(s) 53, 68, 77, 215, 258
BspLI GGNNCC 2 cut(s) 224, 365
BspPI GGATC 3 cut(s) 108, 169, 354
BspTNI GGTCTC 1 cut(s) 244
BsrGI TGTACA 1 cut(s) 105
BssECI CCNNGG 3 cut(s) 111, 279, 354
BssMI GATC 7 cut(s) 36, 57, 100, 174, 216, 253, 346
BssNI GRCGYC 2 cut(s) 155, 384
BssSI CACGAG 1 cut(s) 424
BssT1I CCWWGG 2 cut(s) 111, 279
Bst2BI CACGAG 1 cut(s) 424
Bst2UI CCWGG 1 cut(s) 355
Bst4CI ACNGT 1 cut(s) 376
Bst6I CTCTTC 1 cut(s) 215
BstACI GRCGYC 2 cut(s) 155, 384
BstAUI TGTACA 1 cut(s) 105
BstC8I GCNNGC 2 cut(s) 44, 290
BstDEI CTNAG 2 cut(s) 480, 490
BstFNI CGCG 5 cut(s) 53, 68, 77, 215, 258
BstH2I RGCGCY 1 cut(s) 322
BstHHI GCGC 4 cut(s) 77, 136, 321, 336
BstKTI GATC 7 cut(s) 39, 60, 103, 177, 219, 256, 349
BstMAI GTCTC 3 cut(s) 122, 244, 499
BstMBI GATC 7 cut(s) 36, 57, 100, 174, 216, 253, 346
BstMCI CGRYCG 2 cut(s) 60, 219
BstMWI GCNNNNNNNGC 4 cut(s) 59, 74, 166, 318
BstNI CCWGG 1 cut(s) 355
BstNSI RCATGY 1 cut(s) 97
BstPAI GACNNNNGTC 1 cut(s) 482
BstSCI CCNGG 2 cut(s) 197, 353
BstSLI GKGCMC 1 cut(s) 120
BstUI CGCG 5 cut(s) 53, 68, 77, 215, 258
BstV1I GCAGC 3 cut(s) 272, 308, 437
BstV2I GAAGAC 2 cut(s) 295, 400
BstX2I RGATCY 2 cut(s) 100, 346
BstYI RGATCY 2 cut(s) 100, 346
BsuRI GGCC 2 cut(s) 312, 395
BtgZI GCGATG 1 cut(s) 49
BtuMI TCGCGA 2 cut(s) 215, 258
Cac8I GCNNGC 2 cut(s) 44, 290
CaiI CAGNNNCTG 1 cut(s) 172
CfoI GCGC 4 cut(s) 77, 136, 321, 336
Cfr13I GGNCC 3 cut(s) 180, 310, 394
CseI GACGC 2 cut(s) 66, 163
Csp6I GTAC 1 cut(s) 106
CviAII CATG 5 cut(s) 82, 94, 235, 299, 307
CviJI RGCY 7 cut(s) 142, 169, 270, 285, 312, 364, 395
CviKI_1 RGCY 7 cut(s) 142, 169, 270, 285, 312, 364, 395
CviQI GTAC 1 cut(s) 106
DdeI CTNAG 2 cut(s) 480, 490
DpnI GATC 7 cut(s) 38, 59, 102, 176, 218, 255, 348
DpnII GATC 7 cut(s) 36, 57, 100, 174, 216, 253, 346
Eam1104I CTCTTC 1 cut(s) 215
EarI CTCTTC 1 cut(s) 215
Eco130I CCWWGG 2 cut(s) 111, 279
Eco31I GGTCTC 1 cut(s) 244
Eco47I GGWCC 1 cut(s) 180
Eco88I CYCGRG 2 cut(s) 146, 496
EcoRII CCWGG 1 cut(s) 353
EcoT14I CCWWGG 2 cut(s) 111, 279
ErhI CCWWGG 2 cut(s) 111, 279
Esp3I CGTCTC 1 cut(s) 122
FaeI CATG 5 cut(s) 85, 97, 238, 302, 310
FaiI YATR 6 cut(s) 83, 95, 236, 300, 308, 473
FaqI GGGAC 1 cut(s) 490
FatI CATG 5 cut(s) 81, 93, 234, 298, 306
FblI GTMKAC 1 cut(s) 152
Fnu4HI GCNGC 6 cut(s) 11, 54, 158, 286, 322, 451
Fsp4HI GCNGC 6 cut(s) 11, 54, 158, 286, 322, 451
GlaI GCGC 4 cut(s) 76, 135, 320, 335
GluI GCNGC 6 cut(s) 11, 54, 158, 286, 322, 451
HaeII RGCGCY 1 cut(s) 322
HaeIII GGCC 2 cut(s) 312, 395
HapII CCGG 3 cut(s) 178, 198, 367
HgaI GACGC 2 cut(s) 66, 163
HhaI GCGC 4 cut(s) 77, 136, 321, 336
Hin1I GRCGYC 2 cut(s) 155, 384
Hin1II CATG 5 cut(s) 85, 97, 238, 302, 310
Hin6I GCGC 4 cut(s) 75, 134, 319, 334
HinP1I GCGC 4 cut(s) 75, 134, 319, 334
HincII GTYRAC 2 cut(s) 153, 487
HindII GTYRAC 2 cut(s) 153, 487
HindIII AAGCTT 1 cut(s) 140
HinfI GANTC 2 cut(s) 123, 188
HpaII CCGG 3 cut(s) 178, 198, 367
Hpy166II GTNNAC 3 cut(s) 108, 153, 487
Hpy188I TCNGA 3 cut(s) 100, 187, 251
Hpy188III TCNNGA 7 cut(s) 127, 172, 178, 214, 257, 265, 496
Hpy8I GTNNAC 3 cut(s) 108, 153, 487
Hpy99I CGWCG 7 cut(s) 19, 22, 25, 157, 380, 386, 389
HpyAV CCTTC 1 cut(s) 253
HpyCH4III ACNGT 1 cut(s) 376
HpyCH4IV ACGT 1 cut(s) 384
HpyCH4V TGCA 1 cut(s) 288
HpyF10VI GCNNNNNNNGC 4 cut(s) 59, 74, 166, 318
HpyF3I CTNAG 2 cut(s) 480, 490
HpySE526I ACGT 1 cut(s) 384
Hsp92I GRCGYC 2 cut(s) 155, 384
Hsp92II CATG 5 cut(s) 85, 97, 238, 302, 310
HspAI GCGC 4 cut(s) 75, 134, 319, 334
Kpn2I TCCGGA 1 cut(s) 177
Kzo9I GATC 7 cut(s) 36, 57, 100, 174, 216, 253, 346
LmnI GCTCC 6 cut(s) 51, 174, 210, 234, 297, 369
Lsp1109I GCAGC 3 cut(s) 272, 308, 437
MaeII ACGT 1 cut(s) 384
MalI GATC 7 cut(s) 38, 59, 102, 176, 218, 255, 348
MboI GATC 7 cut(s) 36, 57, 100, 174, 216, 253, 346
MboII GAAGA 5 cut(s) 202, 265, 295, 400, 403
MflI RGATCY 2 cut(s) 100, 346
MhlI GDGCHC 1 cut(s) 120
MluCI AATT 1 cut(s) 442
MlyI GAGTC 2 cut(s) 132, 182
MmeI TCCRAC 2 cut(s) 78, 210
MnlI CCTC 8 cut(s) 193, 218, 242, 305, 323, 337, 407, 475
MroI TCCGGA 1 cut(s) 177
MspI CCGG 3 cut(s) 178, 198, 367
MspR9I CCNGG 2 cut(s) 199, 355
MvaI CCWGG 1 cut(s) 355
MvnI CGCG 5 cut(s) 53, 68, 77, 215, 258
MwoI GCNNNNNNNGC 4 cut(s) 59, 74, 166, 318
NciI CCSGG 1 cut(s) 199
NdeII GATC 7 cut(s) 36, 57, 100, 174, 216, 253, 346
NlaIII CATG 5 cut(s) 85, 97, 238, 302, 310
NlaIV GGNNCC 2 cut(s) 224, 365
NruI TCGCGA 2 cut(s) 215, 258
NspI RCATGY 1 cut(s) 97
PaeR7I CTCGAG 1 cut(s) 496
PciI ACATGT 1 cut(s) 93
PcsI WCGNNNNNNNCGW 3 cut(s) 14, 23, 384
PkrI GCNGC 6 cut(s) 12, 55, 159, 287, 323, 452
Ple19I CGATCG 2 cut(s) 60, 219
PleI GAGTC 2 cut(s) 131, 182
PpsI GAGTC 2 cut(s) 131, 182
PscI ACATGT 1 cut(s) 93
PshAI GACNNNNGTC 1 cut(s) 482
Psp6I CCWGG 1 cut(s) 353
PspGI CCWGG 1 cut(s) 353
PspN4I GGNNCC 2 cut(s) 224, 365
PspPI GGNCC 3 cut(s) 180, 310, 394
PstNI CAGNNNCTG 1 cut(s) 172
PsuI RGATCY 2 cut(s) 100, 346
PvuI CGATCG 2 cut(s) 60, 219
RgaI GCGATCGC 1 cut(s) 60
RruI TCGCGA 2 cut(s) 215, 258
RsaI GTAC 1 cut(s) 107
RsaNI GTAC 1 cut(s) 106
SalI GTCGAC 1 cut(s) 151
SatI GCNGC 6 cut(s) 11, 54, 158, 286, 322, 451
Sau3AI GATC 7 cut(s) 36, 57, 100, 174, 216, 253, 346
Sau96I GGNCC 3 cut(s) 180, 310, 394
SchI GAGTC 2 cut(s) 132, 182
ScrFI CCNGG 2 cut(s) 199, 355
SduI GDGCHC 1 cut(s) 120
SetI ASST 7 cut(s) 113, 144, 171, 185, 272, 387, 486
SfaAI GCGATCGC 1 cut(s) 60
Sfr274I CTCGAG 1 cut(s) 496
SgfI GCGATCGC 1 cut(s) 60
SinI GGWCC 1 cut(s) 180
SlaI CTCGAG 1 cut(s) 496
SmlI CTYRAG 1 cut(s) 496
SmoI CTYRAG 1 cut(s) 496
Sse9I AATT 1 cut(s) 442
SsiI CCGC 3 cut(s) 11, 53, 158
StyD4I CCNGG 2 cut(s) 197, 353
StyI CCWWGG 2 cut(s) 111, 279
TaaI ACNGT 1 cut(s) 376
TaiI ACGT 1 cut(s) 387
TaqI TCGA 5 cut(s) 126, 152, 330, 342, 497
TasI AATT 1 cut(s) 442
TatI WGTACW 1 cut(s) 105
TauI GCSGC 3 cut(s) 13, 56, 160
TseI GCWGC 3 cut(s) 285, 321, 450
TspDTI ATGAA 2 cut(s) 295, 455
TspGWI ACGGA 2 cut(s) 22, 135
VpaK11BI GGWCC 1 cut(s) 180
XceI RCATGY 1 cut(s) 97
XhoI CTCGAG 1 cut(s) 496
XmiI GTMKAC 1 cut(s) 152
ZraI GACGTC 1 cut(s) 385
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.