pycom05g29020

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0003774
Physical Location & Seq
Forward (+)
29570143 .. 29570589
447 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g29020.1

Sequence Viewer

Length: 447 bp
ATGTCGTCGCCGGCGCCGTCGTCGGCCTTCTTCGTCATCTGCCTCCTCCACTCCCTAATCGCCATATCCTGCGGGGCACTCATGATGTTCTACATGACGGAAGTTTACACCTTCGGGCATGGGATCGAGACCGCCCAGAAGCTGCTGGGGTCCACCCCCCACGACCAGCTCCTGATTCGGACCTGCGACTCCTTCTCCGGCCTCCTCCTCTTCGCGATCGGCTTCCTGCTGCTCATGGTCTCCTTCGTGAAAGATTGCGAGTTCCAGAGCTTCTTCGCCAAGGGGTGCACGTTGCTCCACGTTTTCATGGCGCTGTGGAGGTGCTACTTCGAGCGGCGGGTGGAGGACCTCGCCTGGGACTGGCTGAGGCAGACGGTCGGCGACGTCGTGCTGGCGGCGTCTTGGGTTTTCTTCCTTATTTTCTCCTGGAGAGAAAAGTACGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

149

Amino Acids

16.98

Weight (kDa)

5.73

Isoelectric Point (pI)

50.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7865 PF25266 7 - 136 3.3e-69 Domain of unknown function (DUF7865)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 387
Acc36I ACCTGC 1 cut(s) 191
AccB1I GGYRCC 1 cut(s) 13
AccBSI CCGCTC 1 cut(s) 334
AccII CGCG 1 cut(s) 215
AciI CCGC 5 cut(s) 72, 132, 334, 337, 395
AclWI GGATC 1 cut(s) 131
AcyI GRCGYC 3 cut(s) 14, 384, 398
AfaI GTAC 1 cut(s) 440
AfiI CCNNNNNNNGG 2 cut(s) 355, 360
AjnI CCWGG 2 cut(s) 353, 425
AluBI AGCT 3 cut(s) 142, 169, 270
AluI AGCT 3 cut(s) 142, 169, 270
Alw21I GWGCWC 1 cut(s) 290
Alw26I GTCTC 2 cut(s) 122, 244
Alw44I GTGCAC 1 cut(s) 286
AlwI GGATC 1 cut(s) 131
AlwNI CAGNNNCTG 2 cut(s) 142, 172
AoxI GGCC 2 cut(s) 24, 199
ApaLI GTGCAC 1 cut(s) 286
ApeKI GCWGC 2 cut(s) 142, 229
AspLEI GCGC 2 cut(s) 16, 313
AspS9I GGNCC 3 cut(s) 150, 180, 346
AvaII GGWCC 3 cut(s) 150, 180, 346
BaeGI GKGCMC 2 cut(s) 79, 290
BanI GGYRCC 1 cut(s) 13
Bbv12I GWGCWC 1 cut(s) 290
BbvCI CCTCAGC 1 cut(s) 365
BbvI GCAGC 2 cut(s) 129, 216
BciT130I CCWGG 2 cut(s) 355, 427
BcoDI GTCTC 2 cut(s) 122, 244
BfoI RGCGCY 2 cut(s) 17, 314
BfuAI ACCTGC 1 cut(s) 191
BisI GCNGC 4 cut(s) 143, 230, 335, 396
BlsI GCNGC 4 cut(s) 144, 231, 336, 397
Bme1390I CCNGG 2 cut(s) 355, 427
Bme18I GGWCC 3 cut(s) 150, 180, 346
BmgT120I GGNCC 3 cut(s) 150, 180, 346
BmiI GGNNCC 2 cut(s) 15, 151
BmrFI CCNGG 2 cut(s) 355, 427
Bpu10I CCTNAGC 1 cut(s) 365
BsaHI GRCGYC 3 cut(s) 14, 384, 398
BsaI GGTCTC 2 cut(s) 122, 244
BsaJI CCNNGG 2 cut(s) 279, 354
BsaXI ACNNNNNCTCC 2 cut(s) 179, 209
Bsc4I CCNNNNNNNGG 2 cut(s) 355, 360
Bse118I RCCGGY 1 cut(s) 10
Bse1I ACTGG 1 cut(s) 365
BseBI CCWGG 2 cut(s) 355, 427
BseDI CCNNGG 2 cut(s) 279, 354
BseLI CCNNNNNNNGG 2 cut(s) 355, 360
BseMII CTCAG 1 cut(s) 356
BseNI ACTGG 1 cut(s) 365
BseRI GAGGAG 3 cut(s) 35, 194, 197
BseSI GKGCMC 2 cut(s) 79, 290
BseXI GCAGC 2 cut(s) 129, 216
BseYI CCCAGC 1 cut(s) 145
Bsh1236I CGCG 1 cut(s) 215
Bsh1285I CGRYCG 2 cut(s) 219, 378
BshFI GGCC 2 cut(s) 26, 201
BshNI GGYRCC 1 cut(s) 13
BsiEI CGRYCG 2 cut(s) 219, 378
BsiHKAI GWGCWC 1 cut(s) 290
BsiSI CCGG 2 cut(s) 11, 198
BslFI GGGAC 1 cut(s) 371
BslI CCNNNNNNNGG 2 cut(s) 355, 360
BsmAI GTCTC 2 cut(s) 122, 244
BsmFI GGGAC 1 cut(s) 371
BsnI GGCC 2 cut(s) 26, 201
Bso31I GGTCTC 2 cut(s) 122, 244
Bsp1286I GDGCHC 2 cut(s) 79, 290
Bsp143I GATC 2 cut(s) 123, 216
Bsp68I TCGCGA 1 cut(s) 215
BspACI CCGC 5 cut(s) 72, 132, 334, 337, 395
BspANI GGCC 2 cut(s) 26, 201
BspCNI CTCAG 1 cut(s) 357
BspFNI CGCG 1 cut(s) 215
BspHI TCATGA 1 cut(s) 81
BspLI GGNNCC 2 cut(s) 15, 151
BspMI ACCTGC 1 cut(s) 191
BspPI GGATC 1 cut(s) 131
BspT107I GGYRCC 1 cut(s) 13
BspTNI GGTCTC 2 cut(s) 122, 244
BsrBI CCGCTC 1 cut(s) 334
BsrFI RCCGGY 1 cut(s) 10
BsrI ACTGG 1 cut(s) 365
BssAI RCCGGY 1 cut(s) 10
BssECI CCNNGG 2 cut(s) 279, 354
BssMI GATC 2 cut(s) 123, 216
BssNI GRCGYC 3 cut(s) 14, 384, 398
BssT1I CCWWGG 1 cut(s) 279
Bst2UI CCWGG 2 cut(s) 355, 427
Bst4CI ACNGT 1 cut(s) 376
Bst6I CTCTTC 1 cut(s) 215
BstACI GRCGYC 3 cut(s) 14, 384, 398
BstC8I GCNNGC 2 cut(s) 12, 393
BstDEI CTNAG 1 cut(s) 365
BstFNI CGCG 1 cut(s) 215
BstH2I RGCGCY 2 cut(s) 17, 314
BstHHI GCGC 2 cut(s) 16, 313
BstKTI GATC 2 cut(s) 126, 219
BstMAI GTCTC 2 cut(s) 122, 244
BstMBI GATC 2 cut(s) 123, 216
BstMCI CGRYCG 2 cut(s) 219, 378
BstNI CCWGG 2 cut(s) 355, 427
BstSCI CCNGG 2 cut(s) 353, 425
BstSLI GKGCMC 2 cut(s) 79, 290
BstUI CGCG 1 cut(s) 215
BstV1I GCAGC 2 cut(s) 129, 216
BsuRI GGCC 2 cut(s) 26, 201
BtuMI TCGCGA 1 cut(s) 215
BveI ACCTGC 1 cut(s) 191
Cac8I GCNNGC 2 cut(s) 12, 393
CaiI CAGNNNCTG 2 cut(s) 142, 172
CciI TCATGA 1 cut(s) 81
CfoI GCGC 2 cut(s) 16, 313
Cfr10I RCCGGY 1 cut(s) 10
Cfr13I GGNCC 3 cut(s) 150, 180, 346
CseI GACGC 1 cut(s) 387
Csp6I GTAC 1 cut(s) 439
CviAII CATG 5 cut(s) 82, 94, 119, 235, 307
CviJI RGCY 7 cut(s) 26, 142, 169, 201, 222, 270, 364
CviKI_1 RGCY 7 cut(s) 26, 142, 169, 201, 222, 270, 364
CviQI GTAC 1 cut(s) 439
DdeI CTNAG 1 cut(s) 365
DinI GGCGCC 1 cut(s) 15
DpnI GATC 2 cut(s) 125, 218
DpnII GATC 2 cut(s) 123, 216
Eam1104I CTCTTC 1 cut(s) 215
EarI CTCTTC 1 cut(s) 215
Eco130I CCWWGG 1 cut(s) 279
Eco31I GGTCTC 2 cut(s) 122, 244
Eco47I GGWCC 3 cut(s) 150, 180, 346
EcoO109I RGGNCCY 1 cut(s) 346
EcoRII CCWGG 2 cut(s) 353, 425
EcoT14I CCWWGG 1 cut(s) 279
EgeI GGCGCC 1 cut(s) 15
EheI GGCGCC 1 cut(s) 15
ErhI CCWWGG 1 cut(s) 279
FaeI CATG 5 cut(s) 85, 97, 122, 238, 310
FaiI YATR 6 cut(s) 65, 83, 95, 120, 236, 308
FaqI GGGAC 1 cut(s) 371
FatI CATG 5 cut(s) 81, 93, 118, 234, 306
FauI CCCGC 2 cut(s) 65, 330
Fnu4HI GCNGC 4 cut(s) 143, 230, 335, 396
Fsp4HI GCNGC 4 cut(s) 143, 230, 335, 396
GlaI GCGC 2 cut(s) 15, 312
GluI GCNGC 4 cut(s) 143, 230, 335, 396
GsaI CCCAGC 1 cut(s) 149
HaeII RGCGCY 2 cut(s) 17, 314
HaeIII GGCC 2 cut(s) 26, 201
HapII CCGG 2 cut(s) 11, 198
HgaI GACGC 1 cut(s) 387
HhaI GCGC 2 cut(s) 16, 313
Hin1I GRCGYC 3 cut(s) 14, 384, 398
Hin1II CATG 5 cut(s) 85, 97, 122, 238, 310
Hin6I GCGC 2 cut(s) 14, 311
HinP1I GCGC 2 cut(s) 14, 311
HinfI GANTC 2 cut(s) 175, 188
HpaII CCGG 2 cut(s) 11, 198
Hpy166II GTNNAC 3 cut(s) 106, 153, 288
Hpy188I TCNGA 1 cut(s) 180
Hpy188III TCNNGA 6 cut(s) 82, 127, 172, 214, 247, 265
Hpy8I GTNNAC 3 cut(s) 106, 153, 288
Hpy99I CGWCG 5 cut(s) 10, 22, 25, 386, 389
HpyAV CCTTC 4 cut(s) 37, 121, 202, 253
HpyCH4III ACNGT 1 cut(s) 376
HpyCH4IV ACGT 3 cut(s) 290, 300, 384
HpyCH4V TGCA 1 cut(s) 288
HpyF3I CTNAG 1 cut(s) 365
HpySE526I ACGT 3 cut(s) 290, 300, 384
Hsp92I GRCGYC 3 cut(s) 14, 384, 398
Hsp92II CATG 5 cut(s) 85, 97, 122, 238, 310
HspAI GCGC 2 cut(s) 14, 311
KasI GGCGCC 1 cut(s) 13
KroI GCCGGC 1 cut(s) 10
KroNI GCCGGC 1 cut(s) 12
Kzo9I GATC 2 cut(s) 123, 216
LmnI GCTCC 2 cut(s) 174, 300
Lsp1109I GCAGC 2 cut(s) 129, 216
MaeII ACGT 3 cut(s) 290, 300, 384
MalI GATC 2 cut(s) 125, 218
MbiI CCGCTC 1 cut(s) 334
MboI GATC 2 cut(s) 123, 216
MboII GAAGA 4 cut(s) 22, 202, 265, 403
MhlI GDGCHC 2 cut(s) 79, 290
Mly113I GGCGCC 1 cut(s) 14
MlyI GAGTC 1 cut(s) 182
MnlI CCTC 9 cut(s) 53, 56, 212, 215, 218, 312, 337, 359, 360
MreI CGCCGGCG 1 cut(s) 10
MroNI GCCGGC 1 cut(s) 10
MspI CCGG 2 cut(s) 11, 198
MspR9I CCNGG 2 cut(s) 355, 427
MvaI CCWGG 2 cut(s) 355, 427
MvnI CGCG 1 cut(s) 215
NaeI GCCGGC 1 cut(s) 12
NarI GGCGCC 1 cut(s) 14
NdeII GATC 2 cut(s) 123, 216
NgoMIV GCCGGC 1 cut(s) 10
NlaIII CATG 5 cut(s) 85, 97, 122, 238, 310
NlaIV GGNNCC 2 cut(s) 15, 151
NruI TCGCGA 1 cut(s) 215
PagI TCATGA 1 cut(s) 81
PcsI WCGNNNNNNNCGW 2 cut(s) 14, 384
PdiI GCCGGC 1 cut(s) 12
PfeI GAWTC 1 cut(s) 175
PfoI TCCNGGA 1 cut(s) 425
PkrI GCNGC 4 cut(s) 144, 231, 336, 397
Ple19I CGATCG 1 cut(s) 219
PleI GAGTC 1 cut(s) 182
PluTI GGCGCC 1 cut(s) 17
PpsI GAGTC 1 cut(s) 182
PpuMI RGGWCCY 1 cut(s) 346
Psp5II RGGWCCY 1 cut(s) 346
Psp6I CCWGG 2 cut(s) 353, 425
PspFI CCCAGC 1 cut(s) 145
PspGI CCWGG 2 cut(s) 353, 425
PspN4I GGNNCC 2 cut(s) 15, 151
PspPI GGNCC 3 cut(s) 150, 180, 346
PspPPI RGGWCCY 1 cut(s) 346
PstNI CAGNNNCTG 2 cut(s) 142, 172
PvuI CGATCG 1 cut(s) 219
RruI TCGCGA 1 cut(s) 215
RsaI GTAC 1 cut(s) 440
RsaNI GTAC 1 cut(s) 439
SatI GCNGC 4 cut(s) 143, 230, 335, 396
Sau3AI GATC 2 cut(s) 123, 216
Sau96I GGNCC 3 cut(s) 150, 180, 346
SchI GAGTC 1 cut(s) 182
ScrFI CCNGG 2 cut(s) 355, 427
SduI GDGCHC 2 cut(s) 79, 290
SfoI GGCGCC 1 cut(s) 15
SgrAI CRCCGGYG 1 cut(s) 10
SinI GGWCC 3 cut(s) 150, 180, 346
SsiI CCGC 5 cut(s) 72, 132, 334, 337, 395
SspDI GGCGCC 1 cut(s) 13
StyD4I CCNGG 2 cut(s) 353, 425
StyI CCWWGG 1 cut(s) 279
TaaI ACNGT 1 cut(s) 376
TaiI ACGT 3 cut(s) 293, 303, 387
TaqI TCGA 2 cut(s) 126, 330
TauI GCSGC 2 cut(s) 337, 398
TfiI GAWTC 1 cut(s) 175
TseI GCWGC 2 cut(s) 142, 229
TspDTI ATGAA 1 cut(s) 295
TspGWI ACGGA 1 cut(s) 113
VneI GTGCAC 1 cut(s) 286
VpaK11BI GGWCC 3 cut(s) 150, 180, 346
ZraI GACGTC 1 cut(s) 385
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.