Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0002188

Physical Location & Seq

Genomic Coordinates Reverse (-)

19966944 .. 19969757

Gene Structure 2814 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT1G53500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2004 bp

ATGGATGATACTACGTATAAGCCAAAGAACATTCTCATTACTGGAGCTGCTGGATTTATTGCTTCTCATGTTGCCAACAGATTAATCCGTAACTATCCTGATTACAAGATCGTTGTTCTTGACAAGCTTGATTACTGTTCAGATCTGAAGAATCTTGATCCTTCTTTTTCTTCACCAAATTTCAAGTTTGTCAAAGGAGATATCGCGAGTGATGATCTCGTTAACTACCTTCTCATCACTGAAAACATTGATACGATAATGCATTTTGCTGCTCAAACTCATGTTGATAACTCTTTTGGTAATAGCTTTGAGTTTACCAAGAACAATATTTATGGTACTCATGTTCTTTTGGAAGCCTGTAAAGTTACAGGACAGATCAGGAGGTTTATCCATGTGAGTACCGATGAAGTCTATGGAGAAACCGATGAGGATGCTGCTGTAGGAAACCATGAAGCTTCTCAGCTGTTACCGACGAATCCTTACTCTGCAACTAAGGCTGGTGCTGAGATGCTTGTGATGGCTTATGGTAGATCATATGGATTGCCTGTTATTACGACTCGCGGGAACAATGTTTATGGGCCTAACCAGTTTCCTGAAAAAATGATTCCTAAGTTCATCTTGTTGGCTATGAGTGGGAAGCCGCTTCCCATCCATGGAGATGGATCTAATGTCCGGAGTTACTTGTACTGCGAAGACGTTGCTGAGGCTTTTGAGGTTGTTCTTCACAAAGGAGAAATCGGTCATGTCTACAATGTCGGCACAAAAAGAGAAAGGAGAGTGATCGATGTGGCTAGAGACATCTGCAAACTTTTCGGGAAAGACCCTGAGTCAAGCATTCAGTTTGTGGAGAACCGGCCCTTTAATGATCAAAGGTACTTCCTTGATGATCAGAAGCTGAAGAAATTGGGGTGGCAAGAGCGAACAAATTGGGAAGATGGATTGAAGAAGACAATGGACTGGTACACTCAGAATCCTGAGTGGTGGGGTGATGTTTCTGGAGCTTTGCTTCCTCATCCGAGAATGCTTATGATGCCCGGTGGAAGACTTTCTGATGGATCTAGTGAGAAGAAAGACGTTTCAAGCAACACGGTCCAGACATTTACGGTTGTAACACCTAAGAATGGTGATTCTGGTGACAAAGCTTCGTTGAAGTTTTTGATCTATGGTAAGACTGGTTGGCTTGGTGGTCTTCTAGGGAAACTATGTGAGAAGCAAGGGATTACATATGAGTATGGGAAAGGACGTCTGGAGGATAGAGCTTCTCTTGTGGCGGATATTCGTAGCATCAAACCTACTCATGTGTTTAATGCTGCTGGTTTAACTGGCAGACCCAACGTTGACTGGTGTGAATCTCACAAACCAGAGACCATTCGTGTAAATGTCGCAGGTACTTTGACTCTAGCTGATGTTTGCAGAGAGAATGATCTCTTGATGATGAACTTCGCCACCGGTTGCATCTTTGAGTATGACGCTACACATCCTGAGGGTTCGGGTATAGGTTTCAAGGAAGAAGACAAGCCAAATTTCTTTGGTTCTTTCTACTCGAAAACCAAAGCCATGGTTGAGGAGCTCTTGAGAGAATTTGACAATGTATGTACCTTGAGAGTCCGGATGCCAATCTCCTCAGACCTAAACAACCCGAGAAACTTCATCACGAAGATCTCGCGCTACAACAAAGTGGTGGACATCCCGAACAGCATGACCGTACTAGACGAGCTTCTCCCAATCTCTATCGAGATGGCGAAGAGAAACCTAAGAGGCATATGGAATTTCACCAACCCAGGGGTGGTGAGCCACAACGAGATATTGGAGATGTACAAGAATTACATCGAGCCAGGTTTTAAATGGTCCAACTTCACAGTGGAAGAACAAGCAAAGGTCATTGTTGCTGCTCGAAGCAACAACGAAATGGATGGATCTAAACTAAGCAAGGAGTTCCCAGAGATGCTCTCCATCAAAGAGTCACTGCTCAAATACGTCTTTGAACCAAACAAGAGAACCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005911 GO:0006139 GO:0006725 GO:0006793 GO:0006807 GO:0006810 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009225 GO:0009226 GO:0009506 GO:0009791 GO:0009812 GO:0009813 GO:0009914 GO:0009966 GO:0009987 GO:0010154 GO:0010191 GO:0010192 GO:0010214 GO:0010253 GO:0010280 GO:0010315 GO:0010489 GO:0010490 GO:0010646 GO:0010817 GO:0010928 GO:0016491 GO:0016614 GO:0016616 GO:0016829 GO:0016835 GO:0016836 GO:0016853 GO:0016854 GO:0016857 GO:0018130 GO:0019438 GO:0022414 GO:0023051 GO:0030054 GO:0030154 GO:0032501 GO:0032502 GO:0033478 GO:0034641 GO:0034654 GO:0042127 GO:0042440 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046148 GO:0046483 GO:0048316 GO:0048583 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050377 GO:0050789 GO:0050794 GO:0051179 GO:0051234 GO:0051552 GO:0051553 GO:0051554 GO:0051555 GO:0055044 GO:0055086 GO:0055114 GO:0060918 GO:0061458 GO:0065007 GO:0065008 GO:0071704 GO:1901135 GO:1901137 GO:1901360 GO:1901362 GO:1901576 GO:1901615 GO:1901617

KEGG Pathways

Metabolic & Signaling

ko00520 map00520

Pfam Domains

Protein Families

Epimerase GDP_Man_Dehyd

Protein Analysis

667

Amino Acids

75.22

Weight (kDa)

6.04

Isoelectric Point (pI)

29.6

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RmlD_sub_bind	PF04321	10 - 320	2.3e-15	RmlD substrate binding domain
Epimerase	PF01370	11 - 253	1.1e-55	NAD dependent epimerase/dehydratase family
Polysacc_synt_2	PF02719	11 - 283	3.7e-20	Polysaccharide biosynthesis protein
GDP_Man_Dehyd	PF16363	12 - 317	8.8e-66	GDP-mannose 4,6 dehydratase
3Beta_HSD	PF01073	12 - 237	6.3e-23	3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_4	PF07993	13 - 195	1.9e-18	Male sterility protein
RmlD_sub_bind	PF04321	384 - 554	3.7e-13	RmlD substrate binding domain
Epimerase	PF01370	413 - 537	5.6e-09	NAD dependent epimerase/dehydratase family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0002188)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G53500 AT3G14790 AT3G14790
fragaria_vesca	FvH4_2g36650 FvH4_2g36650 FvH4_6g38080 FvH4_6g38080 FvH4_6g38080 FvH4_6g38080 FvH4_6g38080
malus_domestica	MD09G1154300.v1.1 MD09G1154700.v1.1 MD15G1106300.v1.1 MD17G1140800.v1.1
prunus_persica	Prupe.1G458500_v2.0.a1 Prupe.3G170800_v2.0.a1
pyrus_communis	pycom09g07360 pycom15g09690 pycom17g13400
rosa_chinensis	RchiOBHm_Chr2g0151131 RchiOBHm_Chr6g0305141
rosa_laevigata	RLG00000010914 RLG00000020533
rosa_multiflora	Rmu_sc0000061.1_g000019 Rmu_sc0032179.1_g000002
rosa_roxburghii	Rroxscaffold_2G00096710 Rroxscaffold_7G00163120
rosa_rugosa	Rorug02G0425000 Rorug02G0425100 Rorug06G0338000 Rorug06G0338100 Rorug06G0338200 Rorug06G0338300
rosa_samantha	Rh2AG485600 Rh2BG497500 Rh2CG471400 Rh2DG508500 Rh6AG449700 Rh6BG451700 Rh6CG463100 Rh6DG450100
rosa_wichuraiana	Rw2G039770 Rw6G039160

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1246
Acc36I	ACCTGC	1 cut(s)	1376
AccI	GTMKAC	1 cut(s)	747
AccII	CGCG	3 cut(s)	206, 561, 1666
AccIII	TCCGGA	2 cut(s)	672, 1608
AciI	CCGC	3 cut(s)	561, 641, 1271
AclI	AACGTT	1 cut(s)	1335
AclWI	GGATC	4 cut(s)	152, 670, 1063, 1924
AcsI	RAATTY	4 cut(s)	178, 1522, 1580, 1768
AcuI	CTGAAG	2 cut(s)	167, 917
AcyI	GRCGYC	1 cut(s)	1243
AfaI	GTAC	9 cut(s)	337, 400, 686, 875, 962, 1390, 1597, 1707, 1817
AfiI	CCNNNNNNNGG	4 cut(s)	653, 1121, 1782, 1786
AgeI	ACCGGT	1 cut(s)	1448
AgsI	TTSAA	6 cut(s)	184, 943, 1080, 1150, 1504, 1985
AhdI	GACNNNNNGTC	1 cut(s)	826
AjnI	CCWGG	2 cut(s)	1780, 1834
Alw21I	GWGCWC	1 cut(s)	1572
Alw26I	GTCTC	2 cut(s)	789, 1358
AlwI	GGATC	4 cut(s)	152, 670, 1063, 1924
AlwNI	CAGNNNCTG	1 cut(s)	895
Ama87I	CYCGRG	1 cut(s)	1639
Aor13HI	TCCGGA	2 cut(s)	672, 1608
AoxI	GGCC	2 cut(s)	578, 854
ApeKI	GCWGC	5 cut(s)	47, 269, 434, 1310, 1889
ApoI	RAATTY	4 cut(s)	178, 1522, 1580, 1768
AseI	ATTAAT	1 cut(s)	83
AsiGI	ACCGGT	1 cut(s)	1448
Asp700I	GAANNNNTTC	1 cut(s)	1045
AspLEI	GCGC	1 cut(s)	1668
AspS9I	GGNCC	4 cut(s)	578, 855, 1090, 1848
AsuC2I	CCSGG	1 cut(s)	1035
AsuHPI	GGTGA	6 cut(s)	165, 998, 1136, 1145, 1765, 1801
AvaI	CYCGRG	1 cut(s)	1639
AvaII	GGWCC	2 cut(s)	1090, 1848
AxyI	CCTNAGG	1 cut(s)	1482
BaeI	ACNNNNGTAYC	2 cut(s)	327, 360
BanII	GRGCYC	1 cut(s)	1572
BbsI	GAAGAC	5 cut(s)	699, 953, 1048, 1181, 1518
Bbv12I	GWGCWC	1 cut(s)	1572
BbvCI	CCTCAGC	1 cut(s)	702
BbvI	GCAGC	5 cut(s)	34, 256, 421, 1297, 1876
BccI	CCATC	8 cut(s)	511, 653, 656, 929, 1046, 1732, 1907, 1961
BcgI	CGANNNNNNTGC	6 cut(s)	413, 447, 680, 714, 793, 827
BciT130I	CCWGG	2 cut(s)	1782, 1836
BclI	TGATCA	2 cut(s)	865, 886
BcnI	CCSGG	1 cut(s)	1035
BcoDI	GTCTC	2 cut(s)	789, 1358
BfaI	CTAG	5 cut(s)	792, 1059, 1193, 1400, 1709
BfmI	CTRYAG	1 cut(s)	438
BfuAI	ACCTGC	1 cut(s)	1376
BglII	AGATCT	2 cut(s)	142, 1659
BisI	GCNGC	6 cut(s)	48, 270, 435, 641, 1311, 1890
BlsI	GCNGC	6 cut(s)	49, 271, 436, 642, 1312, 1891
Bme1390I	CCNGG	3 cut(s)	1035, 1782, 1836
Bme18I	GGWCC	2 cut(s)	1090, 1848
BmeRI	GACNNNNNGTC	1 cut(s)	826
BmeT110I	CYCGRG	1 cut(s)	1639
BmgT120I	GGNCC	4 cut(s)	578, 855, 1090, 1848
BmrFI	CCNGG	3 cut(s)	1035, 1782, 1836
BmsI	GCATC	7 cut(s)	421, 498, 1020, 1293, 1464, 1602, 1935
BpiI	GAAGAC	5 cut(s)	699, 953, 1048, 1181, 1518
BplI	GAGNNNNNCTC	2 cut(s)	1934, 1966
BpmI	CTGGAG	3 cut(s)	63, 1017, 1268
Bpu10I	CCTNAGC	1 cut(s)	702
BpuEI	CTTGAG	2 cut(s)	1594, 1621
BpuMI	CCSGG	1 cut(s)	1035
Bsa29I	ATCGAT	1 cut(s)	783
BsaAI	YACGTR	1 cut(s)	15
BsaBI	GATNNNNATC	1 cut(s)	1616
BsaHI	GRCGYC	1 cut(s)	1243
BsaI	GGTCTC	1 cut(s)	1358
BsaJI	CCNNGG	4 cut(s)	652, 1557, 1780, 1781
BsaWI	WCCGGW	3 cut(s)	672, 1448, 1608
Bsc4I	CCNNNNNNNGG	4 cut(s)	653, 1121, 1782, 1786
Bse118I	RCCGGY	2 cut(s)	852, 1448
Bse1I	ACTGG	6 cut(s)	46, 586, 962, 1177, 1327, 1346
Bse21I	CCTNAGG	1 cut(s)	1482
Bse8I	GATNNNNATC	1 cut(s)	1616
BseAI	TCCGGA	2 cut(s)	672, 1608
BseBI	CCWGG	2 cut(s)	1782, 1836
BseCI	ATCGAT	1 cut(s)	783
BseDI	CCNNGG	4 cut(s)	652, 1557, 1780, 1781
BseGI	GGATG	8 cut(s)	10, 436, 648, 1012, 1477, 1617, 1686, 1918
BseJI	GATNNNNATC	1 cut(s)	1616
BseLI	CCNNNNNNNGG	4 cut(s)	653, 1121, 1782, 1786
BseMII	CTCAG	8 cut(s)	473, 495, 693, 816, 966, 980, 1473, 1638
BseNI	ACTGG	6 cut(s)	46, 586, 962, 1177, 1327, 1346
BseRI	GAGGAG	2 cut(s)	1580, 1612
BseXI	GCAGC	5 cut(s)	34, 256, 421, 1297, 1876
Bsh1236I	CGCG	3 cut(s)	206, 561, 1666
BshFI	GGCC	2 cut(s)	580, 856
BshTI	ACCGGT	1 cut(s)	1448
BshVI	ATCGAT	1 cut(s)	783
BsiHKAI	GWGCWC	1 cut(s)	1572
BsiHKCI	CYCGRG	1 cut(s)	1639
BsiSI	CCGG	5 cut(s)	673, 853, 1035, 1449, 1609
BslI	CCNNNNNNNGG	4 cut(s)	653, 1121, 1782, 1786
BsmAI	GTCTC	2 cut(s)	789, 1358
BsmI	GAATGC	2 cut(s)	834, 1026
BsnI	GGCC	2 cut(s)	580, 856
Bso31I	GGTCTC	1 cut(s)	1358
BsoBI	CYCGRG	1 cut(s)	1639
Bsp1286I	GDGCHC	1 cut(s)	1572
Bsp13I	TCCGGA	2 cut(s)	672, 1608
Bsp1407I	TGTACA	1 cut(s)	1815
Bsp19I	CCATGG	2 cut(s)	652, 1557
Bsp68I	TCGCGA	1 cut(s)	206
BspACI	CCGC	3 cut(s)	561, 641, 1271
BspANI	GGCC	2 cut(s)	580, 856
BspCNI	CTCAG	8 cut(s)	472, 496, 694, 817, 967, 979, 1474, 1637
BspDI	ATCGAT	1 cut(s)	783
BspEI	TCCGGA	2 cut(s)	672, 1608
BspFNI	CGCG	3 cut(s)	206, 561, 1666
BspMI	ACCTGC	1 cut(s)	1376
BspPI	GGATC	4 cut(s)	152, 670, 1063, 1924
BspTNI	GGTCTC	1 cut(s)	1358
BsrFI	RCCGGY	2 cut(s)	852, 1448
BsrGI	TGTACA	1 cut(s)	1815
BsrI	ACTGG	6 cut(s)	46, 586, 962, 1177, 1327, 1346
BssAI	RCCGGY	2 cut(s)	852, 1448
BssECI	CCNNGG	4 cut(s)	652, 1557, 1780, 1781
BssNI	GRCGYC	1 cut(s)	1243
BssT1I	CCWWGG	2 cut(s)	652, 1557
Bst2UI	CCWGG	2 cut(s)	1782, 1836
Bst4CI	ACNGT	5 cut(s)	137, 1090, 1105, 1705, 1861
Bst6I	CTCTTC	1 cut(s)	1739
BstACI	GRCGYC	1 cut(s)	1243
BstAUI	TGTACA	1 cut(s)	1815
BstBAI	YACGTR	1 cut(s)	15
BstDSI	CCRYGG	2 cut(s)	652, 1557
BstF5I	GGATG	8 cut(s)	10, 436, 648, 1012, 1477, 1617, 1686, 1918
BstFNI	CGCG	3 cut(s)	206, 561, 1666
BstHHI	GCGC	1 cut(s)	1668
BstMAI	GTCTC	2 cut(s)	789, 1358
BstMWI	GCNNNNNNNGC	2 cut(s)	494, 1030
BstNI	CCWGG	2 cut(s)	1782, 1836
BstSCI	CCNGG	3 cut(s)	1033, 1780, 1834
BstSFI	CTRYAG	1 cut(s)	438
BstSNI	TACGTA	1 cut(s)	15
BstUI	CGCG	3 cut(s)	206, 561, 1666
BstV1I	GCAGC	5 cut(s)	34, 256, 421, 1297, 1876
BstV2I	GAAGAC	5 cut(s)	699, 953, 1048, 1181, 1518
BstX2I	RGATCY	5 cut(s)	142, 662, 1055, 1659, 1916
BstXI	CCANNNNNNTGG	2 cut(s)	659, 1558
BstYI	RGATCY	5 cut(s)	142, 662, 1055, 1659, 1916
Bsu15I	ATCGAT	1 cut(s)	783
Bsu36I	CCTNAGG	1 cut(s)	1482
BsuRI	GGCC	2 cut(s)	580, 856
BsuTUI	ATCGAT	1 cut(s)	783
BtgI	CCRYGG	2 cut(s)	652, 1557
BtsCI	GGATG	8 cut(s)	10, 436, 648, 1012, 1477, 1617, 1686, 1918
BtsI	GCAGTG	1 cut(s)	1964
BtsIMutI	CAGTG	3 cut(s)	237, 1866, 1964
BtuMI	TCGCGA	1 cut(s)	206
BveI	ACCTGC	1 cut(s)	1376
CaiI	CAGNNNCTG	1 cut(s)	895
CfoI	GCGC	1 cut(s)	1668
Cfr10I	RCCGGY	2 cut(s)	852, 1448
Cfr13I	GGNCC	4 cut(s)	578, 855, 1090, 1848
ClaI	ATCGAT	1 cut(s)	783
CseI	GACGC	1 cut(s)	1478
Csp6I	GTAC	9 cut(s)	336, 399, 685, 874, 961, 1389, 1596, 1706, 1816
CspAI	ACCGGT	1 cut(s)	1448
CviQI	GTAC	9 cut(s)	336, 399, 685, 874, 961, 1389, 1596, 1706, 1816
DraI	TTTAAA	1 cut(s)	1843
DriI	GACNNNNNGTC	1 cut(s)	826
Eam1104I	CTCTTC	1 cut(s)	1739
Eam1105I	GACNNNNNGTC	1 cut(s)	826
EarI	CTCTTC	1 cut(s)	1739
EciI	GGCGGA	1 cut(s)	1286
Ecl136II	GAGCTC	1 cut(s)	1570
Eco105I	TACGTA	1 cut(s)	15
Eco130I	CCWWGG	2 cut(s)	652, 1557
Eco24I	GRGCYC	1 cut(s)	1572
Eco31I	GGTCTC	1 cut(s)	1358
Eco32I	GATATC	1 cut(s)	202
Eco47I	GGWCC	2 cut(s)	1090, 1848
Eco53kI	GAGCTC	1 cut(s)	1570
Eco57I	CTGAAG	2 cut(s)	167, 917
Eco81I	CCTNAGG	1 cut(s)	1482
Eco88I	CYCGRG	1 cut(s)	1639
EcoICRI	GAGCTC	1 cut(s)	1570
EcoRII	CCWGG	2 cut(s)	1780, 1834
EcoRV	GATATC	1 cut(s)	202
EcoT14I	CCWWGG	2 cut(s)	652, 1557
EcoT22I	ATGCAT	1 cut(s)	264
EcoT38I	GRGCYC	1 cut(s)	1572
ErhI	CCWWGG	2 cut(s)	652, 1557
FalI	AAGNNNNNCTT	2 cut(s)	602, 634
FauI	CCCGC	1 cut(s)	554
FauNDI	CATATG	3 cut(s)	535, 1225, 1763
FbaI	TGATCA	2 cut(s)	865, 886
FblI	GTMKAC	1 cut(s)	747
Fnu4HI	GCNGC	6 cut(s)	48, 270, 435, 641, 1311, 1890
FokI	GGATG	8 cut(s)	17, 443, 635, 999, 1464, 1624, 1673, 1925
FriOI	GRGCYC	1 cut(s)	1572
Fsp4HI	GCNGC	6 cut(s)	48, 270, 435, 641, 1311, 1890
FspBI	CTAG	5 cut(s)	792, 1059, 1193, 1400, 1709
GlaI	GCGC	1 cut(s)	1667
GluI	GCNGC	6 cut(s)	48, 270, 435, 641, 1311, 1890
GsuI	CTGGAG	3 cut(s)	63, 1017, 1268
HaeIII	GGCC	2 cut(s)	580, 856
HapII	CCGG	5 cut(s)	673, 853, 1035, 1449, 1609
HgaI	GACGC	1 cut(s)	1478
HhaI	GCGC	1 cut(s)	1668
Hin1I	GRCGYC	1 cut(s)	1243
Hin6I	GCGC	1 cut(s)	1666
HinP1I	GCGC	1 cut(s)	1666
HincII	GTYRAC	2 cut(s)	223, 1339
HindII	GTYRAC	2 cut(s)	223, 1339
HindIII	AAGCTT	3 cut(s)	125, 453, 1140
HpaI	GTTAAC	1 cut(s)	223
HpaII	CCGG	5 cut(s)	673, 853, 1035, 1449, 1609
HphI	GGTGA	6 cut(s)	165, 998, 1136, 1145, 1765, 1801
Hpy166II	GTNNAC	6 cut(s)	223, 315, 748, 963, 1339, 1684
Hpy188I	TCNGA	7 cut(s)	142, 147, 891, 969, 1017, 1051, 1627
Hpy8I	GTNNAC	6 cut(s)	223, 315, 748, 963, 1339, 1684
Hpy99I	CGWCG	1 cut(s)	475
HpyAV	CCTTC	2 cut(s)	171, 239
HpyCH4III	ACNGT	5 cut(s)	137, 1090, 1105, 1705, 1861
HpyCH4IV	ACGT	6 cut(s)	14, 696, 1074, 1243, 1335, 1977
HpyCH4V	TGCA	5 cut(s)	262, 488, 804, 1413, 1455
HpyF10VI	GCNNNNNNNGC	2 cut(s)	494, 1030
HpySE526I	ACGT	6 cut(s)	14, 696, 1074, 1243, 1335, 1977
Hsp92I	GRCGYC	1 cut(s)	1243
HspAI	GCGC	1 cut(s)	1666
Kpn2I	TCCGGA	2 cut(s)	672, 1608
Ksp22I	TGATCA	2 cut(s)	865, 886
KspAI	GTTAAC	1 cut(s)	223
LmnI	GCTCC	3 cut(s)	44, 998, 1567
Lsp1109I	GCAGC	5 cut(s)	34, 256, 421, 1297, 1876
LweI	GCATC	7 cut(s)	421, 498, 1020, 1293, 1464, 1602, 1935
MaeI	CTAG	5 cut(s)	792, 1059, 1193, 1400, 1709
MaeII	ACGT	6 cut(s)	14, 696, 1074, 1243, 1335, 1977
MaeIII	GTNAC	7 cut(s)	89, 364, 465, 677, 1108, 1133, 1962
MflI	RGATCY	5 cut(s)	142, 662, 1055, 1659, 1916
MhlI	GDGCHC	1 cut(s)	1572
MluCI	AATT	7 cut(s)	178, 902, 925, 1522, 1580, 1768, 1822
MlyI	GAGTC	5 cut(s)	550, 836, 1390, 1614, 1970
MmeI	TCCRAC	1 cut(s)	1875
Mph1103I	ATGCAT	1 cut(s)	264
MroI	TCCGGA	2 cut(s)	672, 1608
MroXI	GAANNNNTTC	1 cut(s)	1045
MseI	TTAA	6 cut(s)	83, 222, 861, 1305, 1319, 1842
MslI	CAYNNNNRTG	1 cut(s)	657
MspA1I	CMGCKG	1 cut(s)	463
MspI	CCGG	5 cut(s)	673, 853, 1035, 1449, 1609
MspR9I	CCNGG	3 cut(s)	1035, 1782, 1836
Mva1269I	GAATGC	2 cut(s)	834, 1026
MvaI	CCWGG	2 cut(s)	1782, 1836
MvnI	CGCG	3 cut(s)	206, 561, 1666
MwoI	GCNNNNNNNGC	2 cut(s)	494, 1030
NciI	CCSGG	1 cut(s)	1035
NcoI	CCATGG	2 cut(s)	652, 1557
NdeI	CATATG	3 cut(s)	535, 1225, 1763
NmuCI	GTSAC	2 cut(s)	1133, 1962
NruI	TCGCGA	1 cut(s)	206
NsiI	ATGCAT	1 cut(s)	264
PasI	CCCWGGG	1 cut(s)	1781
PctI	GAATGC	2 cut(s)	834, 1026
PdmI	GAANNNNTTC	1 cut(s)	1045
PfeI	GAWTC	6 cut(s)	151, 475, 604, 970, 1127, 1349
PinAI	ACCGGT	1 cut(s)	1448
PkrI	GCNGC	6 cut(s)	49, 271, 436, 642, 1312, 1891
PleI	GAGTC	5 cut(s)	550, 835, 1390, 1613, 1969
PpsI	GAGTC	5 cut(s)	550, 835, 1390, 1613, 1969
Ppu21I	YACGTR	1 cut(s)	15
PshBI	ATTAAT	1 cut(s)	83
Psp124BI	GAGCTC	1 cut(s)	1572
Psp1406I	AACGTT	1 cut(s)	1335
Psp6I	CCWGG	2 cut(s)	1780, 1834
PspGI	CCWGG	2 cut(s)	1780, 1834
PspPI	GGNCC	4 cut(s)	578, 855, 1090, 1848
PstNI	CAGNNNCTG	1 cut(s)	895
PsuI	RGATCY	5 cut(s)	142, 662, 1055, 1659, 1916
PvuII	CAGCTG	1 cut(s)	463
RruI	TCGCGA	1 cut(s)	206
RsaI	GTAC	9 cut(s)	337, 400, 686, 875, 962, 1390, 1597, 1707, 1817
RsaNI	GTAC	9 cut(s)	336, 399, 685, 874, 961, 1389, 1596, 1706, 1816
RseI	CAYNNNNRTG	1 cut(s)	657
SacI	GAGCTC	1 cut(s)	1572
SaqAI	TTAA	6 cut(s)	83, 222, 861, 1305, 1319, 1842
SatI	GCNGC	6 cut(s)	48, 270, 435, 641, 1311, 1890
Sau96I	GGNCC	4 cut(s)	578, 855, 1090, 1848
SchI	GAGTC	5 cut(s)	550, 836, 1390, 1614, 1970
ScrFI	CCNGG	3 cut(s)	1035, 1782, 1836
SduI	GDGCHC	1 cut(s)	1572
SfaNI	GCATC	7 cut(s)	421, 498, 1020, 1293, 1464, 1602, 1935
SfcI	CTRYAG	1 cut(s)	438
SinI	GGWCC	2 cut(s)	1090, 1848
SmiMI	CAYNNNNRTG	1 cut(s)	657
SmlI	CTYRAG	2 cut(s)	1573, 1600
SmoI	CTYRAG	2 cut(s)	1573, 1600
SnaBI	TACGTA	1 cut(s)	15
Sse9I	AATT	7 cut(s)	178, 902, 925, 1522, 1580, 1768, 1822
SsiI	CCGC	3 cut(s)	561, 641, 1271
SspI	AATATT	1 cut(s)	328
SspMI	CTAG	5 cut(s)	792, 1059, 1193, 1400, 1709
SstI	GAGCTC	1 cut(s)	1572
StyD4I	CCNGG	3 cut(s)	1033, 1780, 1834
StyI	CCWWGG	2 cut(s)	652, 1557
TaaI	ACNGT	5 cut(s)	137, 1090, 1105, 1705, 1861
TaiI	ACGT	6 cut(s)	17, 699, 1077, 1246, 1338, 1980
TaqI	TCGA	5 cut(s)	783, 1544, 1734, 1830, 1894
TaqII	GACCGA	1 cut(s)	728
TasI	AATT	7 cut(s)	178, 902, 925, 1522, 1580, 1768, 1822
TatI	WGTACW	2 cut(s)	684, 1815
TauI	GCSGC	1 cut(s)	643
TfiI	GAWTC	6 cut(s)	151, 475, 604, 970, 1127, 1349
Tru1I	TTAA	6 cut(s)	83, 222, 861, 1305, 1319, 1842
Tru9I	TTAA	6 cut(s)	83, 222, 861, 1305, 1319, 1842
TscAI	CASTG	3 cut(s)	244, 1866, 1971
TseFI	GTSAC	2 cut(s)	1133, 1962
TseI	GCWGC	5 cut(s)	47, 269, 434, 1310, 1889
Tsp45I	GTSAC	2 cut(s)	1133, 1962
TspDTI	ATGAA	5 cut(s)	420, 465, 604, 1451, 1639
TspGWI	ACGGA	1 cut(s)	77
TspRI	CASTG	3 cut(s)	244, 1866, 1971
VpaK11BI	GGWCC	2 cut(s)	1090, 1848
VspI	ATTAAT	1 cut(s)	83
XapI	RAATTY	4 cut(s)	178, 1522, 1580, 1768
XcmI	CCANNNNNNNNNTGG	3 cut(s)	1783, 1842, 1858
XmiI	GTMKAC	1 cut(s)	747
XmnI	GAANNNNTTC	1 cut(s)	1045
XspI	CTAG	5 cut(s)	792, 1059, 1193, 1400, 1709
ZraI	GACGTC	1 cut(s)	1244
Zsp2I	ATGCAT	1 cut(s)	264

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.