Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0002188

Physical Location & Seq

Genomic Coordinates Forward (+)

12659441 .. 12663161

Gene Structure 3721 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1140800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2238 bp

ATGCATATTTATAAATACCCCATGCTTTACCTTCCCTCCTCACACACACTCATCTCCTTCTCCCTTTTTTTCTGGTCTTTTCTCTTCTTTCTTCTTCAATCTGTCTTAAATCCAGCACAGACAAAGGGCTGCGAATTAAACTCAAGATCCAGTCCAACCCAGAATTATTCCGATTTTTTTAAACTCTTTTGGATCGACATGGATACTGCGTATAAACCGAAGAACATCCTCCTTACTGGAGCTGCTGGCTTCATTGCTTCCCATGTTTGCAACCGGCTTATCCAGAACTACCCTGGCTACAAGATTGTAGTCCTTGACAAGCTTGATTACTGTTCAAATTTGAAGAACCTTAATCCTTCAAAGTCATCCCCCAACCTTAAGTTTATCAAGGGAGACATAGGCAGTGCCGATCTTGTCAACTTCATCCTTTTGACCGAGAACATTGATACAATAATGCACTTTGCAGCCCAGACCCATGTCGACAACTCTTTTGGTAACAGCTTTGAGTTCACCCAAAACAACATCTACGGTACACATGTTCTTCTAGAAGCATGCAAAGTCACCGGTCAAATCAAAAGATTTATTCATGTAAGCACAGATGAAGTCTACGGGGAGACAGATGAGGATGCTGTGGTGGGAAATCATGAGGCTTCTCAGCTTCTTCCAACAAACCCCTACTCTGCAACTAAGGCTGGAGCGGAGATGCTTGTTATGGCATATGGGCGTTCATATGGATTGCCTGTGATAACGACCAGAGGAAACAATGTTTATGGCCCCAACCAGTTCCCTGAAAAGATGATTCCAAAATTCATTCTCTTGGCTATGCAAGGGAAGCCTCTCCCGATTCATGGTGATGGATCCAATGTCAGAAGTTATCTCTATTGTGAGGATGTAGCAGAGGCATTTGAAGTCATTCTTCATAGGGGTGAGGTAGGCCATGTGTACAACATTGGGACGAAGAAAGAGAGGAGGGTGGTTGATGTGGCCAAGGAAATTTGCCAGCTATTTTCTCTGAACCCAGATACTCATGTCAAGTTTGTAGAGAACAGGCCTTTCAATGATCAGAGATATTTCTTGGATGACTCGAAGCTGAAAAGCTTGGGATGGTTGGAAAGGACTTTGTGGGAGGAGGGCTTGAGGAAGACCATGGAATGGTATGTCAAGAACCCCCAATGGTGGGGGGATGTTTCTGGGGCACTGCTCCCGCATCCAAGAATGCTCATGGTTCCTGGAATTGAAAGACAATTTGATGGTTCCGATACTGGCGATTCTGCTTACCCTTTGTCAGCAAGTGATTCTAGTCACAGCAAACTGGTCGTTCCAGCTCCAAGGAACATCCCATCTACTGAGAAGCCATCTCTAAAGTTTTTGATTTATGGTAAAACAGGGTGGATCGGAGGCCTTCTTGGGAAGATTTGTGAGAAGCAAGGGATATCGTGTGAGTATGGACGAGGGCGTCTTCAGGAACGGTCACAGCTCATGGCTGATATTCTGCGTGTCAAACCAACCCATGTTTTCAATGCTGCTGGAGTGACAGGCAGACCCAATGTGGATTGGTGTGAATCTCATAAACCAGAAACAATTCGCACCAATGTTGTTGGTACATTAACCTTGGCTGATGTCTGCAGAGATCACGGCCTCCTAGTGGTGAATTATGCCACTGGTTGCATTTTCGAGTATGATGATGCCCATCCACCAAGATCAGGAATTGGGTTCAAGGAGGAAGACACACCCAATTTCGCGGGCTCATTTTATTCCAAAACCAAAGCCATGGTTGAAGAGCTTCTGAAGGAGTATGACAATGTTTGCACTCTTAGAGTTCGGATGCCAATATCATCTGATCTCAGCAATCCACGCAACTTCATCACAAAGATCTCGCGCTACAATAAAGTGGTCGACATTCCAAACAGCATGACCATCTTGGACGAGCTTCTACCCATTTCAGTTGAGATGGCCAAGAGGAACTTGAGGGGCATCTGGAACTTCACAAACCCGGGCGTGGTAAGTCACAATGAGATCCTGGAGATGTACAAGAAGTACATTGACCCCAGTTTCAAGTGGACCAATTTTACAGTGGAAGAACAAGCCAAGGTTATTGTTGCAGCAAGGAGTAACAATGAGATGGATGCATCAAAGCTGAAGAAAGAGTTCCCTGAACTGCTTCCGATTAAGGAATCGCTGATTAAATACGTGTTTGAACCCAACAAGAAAACATTTGCAGGTGGAGCAGCAATTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005911 GO:0006139 GO:0006725 GO:0006793 GO:0006807 GO:0006810 GO:0008150 GO:0008152 GO:0009058 GO:0009225 GO:0009226 GO:0009506 GO:0009812 GO:0009813 GO:0009914 GO:0009966 GO:0009987 GO:0010253 GO:0010280 GO:0010315 GO:0010646 GO:0010817 GO:0010928 GO:0016829 GO:0016835 GO:0016836 GO:0018130 GO:0019438 GO:0023051 GO:0030054 GO:0030154 GO:0032502 GO:0033478 GO:0034641 GO:0034654 GO:0042127 GO:0042440 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046148 GO:0046483 GO:0048583 GO:0048869 GO:0050377 GO:0050789 GO:0050794 GO:0051179 GO:0051234 GO:0051552 GO:0051553 GO:0051554 GO:0051555 GO:0055044 GO:0055086 GO:0060918 GO:0065007 GO:0065008 GO:0071704 GO:1901135 GO:1901137 GO:1901360 GO:1901362 GO:1901576 GO:1901615 GO:1901617

KEGG Pathways

Metabolic & Signaling

ko00520 map00520

Pfam Domains

Protein Families

Epimerase GDP_Man_Dehyd

Protein Analysis

746

Amino Acids

84.11

Weight (kDa)

7.27

Isoelectric Point (pI)

34.43

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RmlD_sub_bind	PF04321	75 - 384	9.5e-14	RmlD substrate binding domain
Epimerase	PF01370	76 - 318	1.3e-56	NAD dependent epimerase/dehydratase family
Polysacc_synt_2	PF02719	76 - 350	3e-20	Polysaccharide biosynthesis protein
GDP_Man_Dehyd	PF16363	77 - 382	6.8e-64	GDP-mannose 4,6 dehydratase
3Beta_HSD	PF01073	78 - 301	4.2e-23	3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_4	PF07993	78 - 260	1.8e-18	Male sterility protein
RmlD_sub_bind	PF04321	455 - 625	3.3e-12	RmlD substrate binding domain
Epimerase	PF01370	484 - 619	3.5e-11	NAD dependent epimerase/dehydratase family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0002188)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G53500 AT3G14790 AT3G14790
fragaria_vesca	FvH4_2g36650 FvH4_2g36650 FvH4_6g38080 FvH4_6g38080 FvH4_6g38080 FvH4_6g38080 FvH4_6g38080
malus_domestica	MD09G1154300.v1.1 MD09G1154700.v1.1 MD15G1106300.v1.1 MD17G1140800.v1.1
prunus_persica	Prupe.1G458500_v2.0.a1 Prupe.3G170800_v2.0.a1
pyrus_communis	pycom09g07360 pycom15g09690 pycom17g13400
rosa_chinensis	RchiOBHm_Chr2g0151131 RchiOBHm_Chr6g0305141
rosa_laevigata	RLG00000010914 RLG00000020533
rosa_multiflora	Rmu_sc0000061.1_g000019 Rmu_sc0032179.1_g000002
rosa_roxburghii	Rroxscaffold_2G00096710 Rroxscaffold_7G00163120
rosa_rugosa	Rorug02G0425000 Rorug02G0425100 Rorug06G0338000 Rorug06G0338100 Rorug06G0338200 Rorug06G0338300
rosa_samantha	Rh2AG485600 Rh2BG497500 Rh2CG471400 Rh2DG508500 Rh6AG449700 Rh6BG451700 Rh6CG463100 Rh6DG450100
rosa_wichuraiana	Rw2G039770 Rw6G039160

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	12
AarI	CACCTGC	1 cut(s)	2210
AasI	GACNNNNNNGTC	1 cut(s)	1455
Acc36I	ACCTGC	1 cut(s)	2210
AccB7I	CCANNNNNTGG	1 cut(s)	1152
AccBSI	CCGCTC	1 cut(s)	698
AccI	GTMKAC	3 cut(s)	480, 606, 1896
AccII	CGCG	2 cut(s)	1742, 1879
AciI	CCGC	3 cut(s)	698, 1205, 1742
AclWI	GGATC	6 cut(s)	141, 200, 852, 865, 1400, 2011
AcoI	YGGCCR	2 cut(s)	984, 1953
AcsI	RAATTY	3 cut(s)	337, 806, 993
AcuI	CTGAAG	3 cut(s)	1445, 1808, 2159
AcyI	GRCGYC	1 cut(s)	1456
AfaI	GTAC	5 cut(s)	532, 944, 1603, 2030, 2039
AfiI	CCNNNNNNNGG	7 cut(s)	848, 1152, 1176, 1177, 1645, 1703, 1999
AflII	CTTAAG	1 cut(s)	377
AflIII	ACRYGT	2 cut(s)	535, 2190
AgeI	ACCGGT	1 cut(s)	563
AjnI	CCWGG	3 cut(s)	292, 1228, 2019
Alw26I	GTCTC	2 cut(s)	387, 608
AlwI	GGATC	6 cut(s)	141, 200, 852, 865, 1400, 2011
Ama87I	CYCGRG	1 cut(s)	1993
AoxI	GGCC	7 cut(s)	772, 934, 984, 1049, 1399, 1636, 1953
ApeKI	GCWGC	6 cut(s)	129, 242, 464, 1523, 2102, 2228
ApoI	RAATTY	3 cut(s)	337, 806, 993
ArsI	GACNNNNNNTTYG	2 cut(s)	473, 505
AsiGI	ACCGGT	1 cut(s)	563
Asp700I	GAANNNNTTC	5 cut(s)	912, 1581, 1782, 2147, 2160
AspLEI	GCGC	1 cut(s)	1881
AspS9I	GGNCC	2 cut(s)	773, 2061
AsuC2I	CCSGG	2 cut(s)	1994, 1995
AsuHPI	GGTGA	5 cut(s)	502, 553, 863, 938, 1660
AvaI	CYCGRG	1 cut(s)	1993
AvaII	GGWCC	1 cut(s)	2061
BaeGI	GKGCMC	1 cut(s)	1198
BaeI	ACNNNNGTAYC	4 cut(s)	522, 555, 1014, 1047
BalI	TGGCCA	2 cut(s)	986, 1955
BamHI	GGATCC	1 cut(s)	857
BanII	GRGCYC	1 cut(s)	1748
BbsI	GAAGAC	3 cut(s)	1148, 1451, 1731
BbvI	GCAGC	5 cut(s)	116, 229, 476, 1510, 2114
BccI	CCATC	9 cut(s)	848, 1098, 1244, 1348, 1363, 1698, 1925, 1945, 2116
BceAI	ACGGC	1 cut(s)	1651
BcgI	CGANNNNNNTGC	2 cut(s)	1297, 1331
BciT130I	CCWGG	3 cut(s)	294, 1230, 2021
BciVI	GTATCC	1 cut(s)	196
BclI	TGATCA	1 cut(s)	1060
BcnI	CCSGG	2 cut(s)	1994, 1995
BcoDI	GTCTC	2 cut(s)	387, 608
BfaI	CTAG	3 cut(s)	545, 1299, 1643
BfmI	CTRYAG	1 cut(s)	1624
BfrI	CTTAAG	1 cut(s)	377
BfuAI	ACCTGC	1 cut(s)	2210
BfuI	GTATCC	1 cut(s)	196
BglII	AGATCT	1 cut(s)	1872
BisI	GCNGC	6 cut(s)	130, 243, 465, 1524, 2103, 2229
BlsI	GCNGC	6 cut(s)	131, 244, 466, 1525, 2104, 2230
Bme1390I	CCNGG	5 cut(s)	294, 1230, 1994, 1995, 2021
Bme18I	GGWCC	1 cut(s)	2061
BmeT110I	CYCGRG	1 cut(s)	1993
BmgT120I	GGNCC	2 cut(s)	773, 2061
BmiI	GGNNCC	4 cut(s)	775, 859, 1227, 1255
BmrFI	CCNGG	5 cut(s)	294, 1230, 1994, 1995, 2021
BmrI	ACTGGG	1 cut(s)	2043
BmsI	GCATC	8 cut(s)	616, 693, 1216, 1675, 1815, 1983, 2116, 2138
BmuI	ACTGGG	1 cut(s)	2043
BoxI	GACNNNNGTC	1 cut(s)	476
BpiI	GAAGAC	3 cut(s)	1148, 1451, 1731
BpmI	CTGGAG	4 cut(s)	258, 714, 1548, 2042
BpuEI	CTTGAG	3 cut(s)	127, 1156, 1987
BpuMI	CCSGG	2 cut(s)	1994, 1995
BsaAI	YACGTR	1 cut(s)	2191
BsaBI	GATNNNNATC	1 cut(s)	1689
BsaHI	GRCGYC	1 cut(s)	1456
BsaJI	CCNNGG	8 cut(s)	292, 987, 1146, 1328, 1611, 1770, 1993, 2088
BsaWI	WCCGGW	1 cut(s)	563
BsaXI	ACNNNNNCTCC	2 cut(s)	20, 50
Bsc4I	CCNNNNNNNGG	7 cut(s)	848, 1152, 1176, 1177, 1645, 1703, 1999
Bse118I	RCCGGY	2 cut(s)	273, 563
Bse1I	ACTGG	7 cut(s)	150, 241, 781, 1267, 1317, 1666, 2049
Bse3DI	GCAATG	1 cut(s)	252
Bse8I	GATNNNNATC	1 cut(s)	1689
BseBI	CCWGG	3 cut(s)	294, 1230, 2021
BseDI	CCNNGG	8 cut(s)	292, 987, 1146, 1328, 1611, 1770, 1993, 2088
BseJI	GATNNNNATC	1 cut(s)	1689
BseLI	CCNNNNNNNGG	7 cut(s)	848, 1152, 1176, 1177, 1645, 1703, 1999
BseMI	GCAATG	1 cut(s)	252
BseMII	CTCAG	3 cut(s)	668, 1338, 1858
BseNI	ACTGG	7 cut(s)	150, 241, 781, 1267, 1317, 1666, 2049
BseRI	GAGGAG	3 cut(s)	28, 982, 1142
BseSI	GKGCMC	1 cut(s)	1198
BseXI	GCAGC	5 cut(s)	116, 229, 476, 1510, 2114
Bsh1236I	CGCG	2 cut(s)	1742, 1879
BshFI	GGCC	7 cut(s)	774, 936, 986, 1051, 1401, 1638, 1955
BshTI	ACCGGT	1 cut(s)	563
BsiHKCI	CYCGRG	1 cut(s)	1993
BsiSI	CCGG	3 cut(s)	274, 564, 1994
BslFI	GGGAC	1 cut(s)	967
BslI	CCNNNNNNNGG	7 cut(s)	848, 1152, 1176, 1177, 1645, 1703, 1999
BsmAI	GTCTC	2 cut(s)	387, 608
BsmFI	GGGAC	1 cut(s)	967
BsmI	GAATGC	1 cut(s)	1221
BsnI	GGCC	7 cut(s)	774, 936, 986, 1051, 1401, 1638, 1955
BsoBI	CYCGRG	1 cut(s)	1993
Bsp1286I	GDGCHC	2 cut(s)	1198, 1748
Bsp1407I	TGTACA	2 cut(s)	942, 2028
Bsp19I	CCATGG	2 cut(s)	1146, 1770
BspACI	CCGC	3 cut(s)	698, 1205, 1742
BspANI	GGCC	7 cut(s)	774, 936, 986, 1051, 1401, 1638, 1955
BspCNI	CTCAG	3 cut(s)	667, 1339, 1857
BspFNI	CGCG	2 cut(s)	1742, 1879
BspHI	TCATGA	1 cut(s)	643
BspLI	GGNNCC	4 cut(s)	775, 859, 1227, 1255
BspMAI	CTGCAG	1 cut(s)	1628
BspMI	ACCTGC	1 cut(s)	2210
BspPI	GGATC	6 cut(s)	141, 200, 852, 865, 1400, 2011
BspQI	GCTCTTC	1 cut(s)	1773
BspTI	CTTAAG	1 cut(s)	377
BsrBI	CCGCTC	1 cut(s)	698
BsrDI	GCAATG	1 cut(s)	252
BsrFI	RCCGGY	2 cut(s)	273, 563
BsrGI	TGTACA	2 cut(s)	942, 2028
BsrI	ACTGG	7 cut(s)	150, 241, 781, 1267, 1317, 1666, 2049
BssAI	RCCGGY	2 cut(s)	273, 563
BssECI	CCNNGG	8 cut(s)	292, 987, 1146, 1328, 1611, 1770, 1993, 2088
BssNI	GRCGYC	1 cut(s)	1456
BssT1I	CCWWGG	6 cut(s)	987, 1146, 1328, 1611, 1770, 2088
Bst2UI	CCWGG	3 cut(s)	294, 1230, 2021
Bst4CI	ACNGT	4 cut(s)	332, 530, 1470, 2074
Bst6I	CTCTTC	2 cut(s)	89, 1773
BstACI	GRCGYC	1 cut(s)	1456
BstAFI	CTTAAG	1 cut(s)	377
BstAUI	TGTACA	2 cut(s)	942, 2028
BstBAI	YACGTR	1 cut(s)	2191
BstC8I	GCNNGC	4 cut(s)	247, 553, 1001, 1744
BstDEI	CTNAG	5 cut(s)	654, 687, 1347, 1814, 1844
BstDSI	CCRYGG	2 cut(s)	1146, 1770
BstFNI	CGCG	2 cut(s)	1742, 1879
BstHHI	GCGC	1 cut(s)	1881
BstMAI	GTCTC	2 cut(s)	387, 608
BstMWI	GCNNNNNNNGC	4 cut(s)	689, 832, 1854, 2225
BstNI	CCWGG	3 cut(s)	294, 1230, 2021
BstNSI	RCATGY	2 cut(s)	539, 555
BstPAI	GACNNNNGTC	1 cut(s)	476
BstSCI	CCNGG	5 cut(s)	292, 1228, 1992, 1993, 2019
BstSFI	CTRYAG	1 cut(s)	1624
BstSLI	GKGCMC	1 cut(s)	1198
BstUI	CGCG	2 cut(s)	1742, 1879
BstV1I	GCAGC	5 cut(s)	116, 229, 476, 1510, 2114
BstV2I	GAAGAC	3 cut(s)	1148, 1451, 1731
BstX2I	RGATCY	4 cut(s)	146, 857, 1872, 2016
BstXI	CCANNNNNNTGG	1 cut(s)	1771
BstYI	RGATCY	4 cut(s)	146, 857, 1872, 2016
BsuI	GTATCC	1 cut(s)	196
BsuRI	GGCC	7 cut(s)	774, 936, 986, 1051, 1401, 1638, 1955
BtgI	CCRYGG	2 cut(s)	1146, 1770
BtsI	GCAGTG	2 cut(s)	409, 1196
BtsIMutI	CAGTG	4 cut(s)	409, 1196, 1659, 2079
BveI	ACCTGC	1 cut(s)	2210
Cac8I	GCNNGC	4 cut(s)	247, 553, 1001, 1744
CciI	TCATGA	1 cut(s)	643
CfoI	GCGC	1 cut(s)	1881
Cfr10I	RCCGGY	2 cut(s)	273, 563
Cfr13I	GGNCC	2 cut(s)	773, 2061
Cfr9I	CCCGGG	1 cut(s)	1993
CseI	GACGC	1 cut(s)	1445
Csp6I	GTAC	5 cut(s)	531, 943, 1602, 2029, 2038
CspAI	ACCGGT	1 cut(s)	563
CviQI	GTAC	5 cut(s)	531, 943, 1602, 2029, 2038
DdeI	CTNAG	5 cut(s)	654, 687, 1347, 1814, 1844
DraI	TTTAAA	1 cut(s)	181
DrdI	GACNNNNNNGTC	1 cut(s)	1455
DseDI	GACNNNNNNGTC	1 cut(s)	1455
EaeI	YGGCCR	2 cut(s)	984, 1953
Eam1104I	CTCTTC	2 cut(s)	89, 1773
EarI	CTCTTC	2 cut(s)	89, 1773
Eco130I	CCWWGG	6 cut(s)	987, 1146, 1328, 1611, 1770, 2088
Eco147I	AGGCCT	2 cut(s)	1051, 1401
Eco24I	GRGCYC	1 cut(s)	1748
Eco32I	GATATC	1 cut(s)	1434
Eco47I	GGWCC	1 cut(s)	2061
Eco57I	CTGAAG	3 cut(s)	1445, 1808, 2159
Eco88I	CYCGRG	1 cut(s)	1993
EcoRII	CCWGG	3 cut(s)	292, 1228, 2019
EcoRV	GATATC	1 cut(s)	1434
EcoT14I	CCWWGG	6 cut(s)	987, 1146, 1328, 1611, 1770, 2088
EcoT22I	ATGCAT	2 cut(s)	6, 2131
EcoT38I	GRGCYC	1 cut(s)	1748
ErhI	CCWWGG	6 cut(s)	987, 1146, 1328, 1611, 1770, 2088
FalI	AAGNNNNNCTT	4 cut(s)	900, 932, 1949, 1981
FaqI	GGGAC	1 cut(s)	967
FauI	CCCGC	2 cut(s)	1212, 1735
FauNDI	CATATG	2 cut(s)	718, 730
FbaI	TGATCA	1 cut(s)	1060
FblI	GTMKAC	3 cut(s)	480, 606, 1896
Fnu4HI	GCNGC	6 cut(s)	130, 243, 465, 1524, 2103, 2229
FriOI	GRGCYC	1 cut(s)	1748
Fsp4HI	GCNGC	6 cut(s)	130, 243, 465, 1524, 2103, 2229
FspBI	CTAG	3 cut(s)	545, 1299, 1643
GlaI	GCGC	1 cut(s)	1880
GluI	GCNGC	6 cut(s)	130, 243, 465, 1524, 2103, 2229
GsuI	CTGGAG	4 cut(s)	258, 714, 1548, 2042
HaeIII	GGCC	7 cut(s)	774, 936, 986, 1051, 1401, 1638, 1955
HapII	CCGG	3 cut(s)	274, 564, 1994
HgaI	GACGC	1 cut(s)	1445
HhaI	GCGC	1 cut(s)	1881
Hin1I	GRCGYC	1 cut(s)	1456
Hin6I	GCGC	1 cut(s)	1879
HinP1I	GCGC	1 cut(s)	1879
HincII	GTYRAC	3 cut(s)	418, 481, 1897
HindII	GTYRAC	3 cut(s)	418, 481, 1897
HindIII	AAGCTT	2 cut(s)	320, 1096
HinfI	GANTC	7 cut(s)	799, 844, 1082, 1268, 1295, 1562, 2174
HpaII	CCGG	3 cut(s)	274, 564, 1994
HphI	GGTGA	5 cut(s)	502, 553, 863, 938, 1660
Hpy166II	GTNNAC	8 cut(s)	418, 481, 510, 533, 607, 943, 1897, 2061
Hpy188III	TCNNGA	9 cut(s)	144, 283, 545, 644, 841, 1162, 1463, 1704, 1978
Hpy8I	GTNNAC	8 cut(s)	418, 481, 510, 533, 607, 943, 1897, 2061
HpyAV	CCTTC	5 cut(s)	41, 67, 366, 1412, 1783
HpyCH4III	ACNGT	4 cut(s)	332, 530, 1470, 2074
HpyCH4IV	ACGT	1 cut(s)	2190
HpyF10VI	GCNNNNNNNGC	4 cut(s)	689, 832, 1854, 2225
HpyF3I	CTNAG	5 cut(s)	654, 687, 1347, 1814, 1844
HpySE526I	ACGT	1 cut(s)	2190
Hsp92I	GRCGYC	1 cut(s)	1456
HspAI	GCGC	1 cut(s)	1879
Ksp22I	TGATCA	1 cut(s)	1060
LguI	GCTCTTC	1 cut(s)	1773
LmnI	GCTCC	5 cut(s)	239, 695, 1206, 1330, 2225
Lsp1109I	GCAGC	5 cut(s)	116, 229, 476, 1510, 2114
LweI	GCATC	8 cut(s)	616, 693, 1216, 1675, 1815, 1983, 2116, 2138
MaeI	CTAG	3 cut(s)	545, 1299, 1643
MaeII	ACGT	1 cut(s)	2190
MaeIII	GTNAC	7 cut(s)	494, 559, 1301, 1470, 1531, 2006, 2111
MbiI	CCGCTC	1 cut(s)	698
MflI	RGATCY	4 cut(s)	146, 857, 1872, 2016
MhlI	GDGCHC	2 cut(s)	1198, 1748
MlsI	TGGCCA	2 cut(s)	986, 1955
MluNI	TGGCCA	2 cut(s)	986, 1955
MlyI	GAGTC	1 cut(s)	1076
MmeI	TCCRAC	3 cut(s)	179, 689, 1089
Mox20I	TGGCCA	2 cut(s)	986, 1955
Mph1103I	ATGCAT	2 cut(s)	6, 2131
MroXI	GAANNNNTTC	5 cut(s)	912, 1581, 1782, 2147, 2160
MscI	TGGCCA	2 cut(s)	986, 1955
MseI	TTAA	9 cut(s)	107, 137, 180, 351, 378, 1607, 2169, 2184, 2236
MslI	CAYNNNNRTG	2 cut(s)	852, 924
Msp20I	TGGCCA	2 cut(s)	986, 1955
MspCI	CTTAAG	1 cut(s)	377
MspI	CCGG	3 cut(s)	274, 564, 1994
MspR9I	CCNGG	5 cut(s)	294, 1230, 1994, 1995, 2021
Mva1269I	GAATGC	1 cut(s)	1221
MvaI	CCWGG	3 cut(s)	294, 1230, 2021
MvnI	CGCG	2 cut(s)	1742, 1879
MwoI	GCNNNNNNNGC	4 cut(s)	689, 832, 1854, 2225
NciI	CCSGG	2 cut(s)	1994, 1995
NcoI	CCATGG	2 cut(s)	1146, 1770
NdeI	CATATG	2 cut(s)	718, 730
NlaIV	GGNNCC	4 cut(s)	775, 859, 1227, 1255
NmuCI	GTSAC	5 cut(s)	559, 1301, 1470, 1531, 2006
NsiI	ATGCAT	2 cut(s)	6, 2131
NspI	RCATGY	2 cut(s)	539, 555
PaeI	GCATGC	1 cut(s)	555
PagI	TCATGA	1 cut(s)	643
PaqCI	CACCTGC	1 cut(s)	2210
PceI	AGGCCT	2 cut(s)	1051, 1401
PciI	ACATGT	1 cut(s)	535
PciSI	GCTCTTC	1 cut(s)	1773
PctI	GAATGC	1 cut(s)	1221
PdmI	GAANNNNTTC	5 cut(s)	912, 1581, 1782, 2147, 2160
PfeI	GAWTC	6 cut(s)	799, 844, 1268, 1295, 1562, 2174
PflMI	CCANNNNNTGG	1 cut(s)	1152
PfoI	TCCNGGA	2 cut(s)	1228, 2019
PinAI	ACCGGT	1 cut(s)	563
PkrI	GCNGC	6 cut(s)	131, 244, 466, 1525, 2104, 2230
PleI	GAGTC	1 cut(s)	1076
PpsI	GAGTC	1 cut(s)	1076
Ppu21I	YACGTR	1 cut(s)	2191
PscI	ACATGT	1 cut(s)	535
PshAI	GACNNNNGTC	1 cut(s)	476
PsiI	TTATAA	1 cut(s)	12
Psp6I	CCWGG	3 cut(s)	292, 1228, 2019
PspGI	CCWGG	3 cut(s)	292, 1228, 2019
PspN4I	GGNNCC	4 cut(s)	775, 859, 1227, 1255
PspPI	GGNCC	2 cut(s)	773, 2061
PstI	CTGCAG	1 cut(s)	1628
PsuI	RGATCY	4 cut(s)	146, 857, 1872, 2016
RsaI	GTAC	5 cut(s)	532, 944, 1603, 2030, 2039
RsaNI	GTAC	5 cut(s)	531, 943, 1602, 2029, 2038
RseI	CAYNNNNRTG	2 cut(s)	852, 924
SalI	GTCGAC	2 cut(s)	479, 1895
SapI	GCTCTTC	1 cut(s)	1773
SaqAI	TTAA	9 cut(s)	107, 137, 180, 351, 378, 1607, 2169, 2184, 2236
SatI	GCNGC	6 cut(s)	130, 243, 465, 1524, 2103, 2229
Sau96I	GGNCC	2 cut(s)	773, 2061
SchI	GAGTC	1 cut(s)	1076
ScrFI	CCNGG	5 cut(s)	294, 1230, 1994, 1995, 2021
SduI	GDGCHC	2 cut(s)	1198, 1748
SfaNI	GCATC	8 cut(s)	616, 693, 1216, 1675, 1815, 1983, 2116, 2138
SfcI	CTRYAG	1 cut(s)	1624
SinI	GGWCC	1 cut(s)	2061
SmaI	CCCGGG	1 cut(s)	1995
SmiMI	CAYNNNNRTG	2 cut(s)	852, 924
SmlI	CTYRAG	4 cut(s)	142, 377, 1135, 1966
SmoI	CTYRAG	4 cut(s)	142, 377, 1135, 1966
SphI	GCATGC	1 cut(s)	555
SseBI	AGGCCT	2 cut(s)	1051, 1401
SsiI	CCGC	3 cut(s)	698, 1205, 1742
SspMI	CTAG	3 cut(s)	545, 1299, 1643
StuI	AGGCCT	2 cut(s)	1051, 1401
StyD4I	CCNGG	5 cut(s)	292, 1228, 1992, 1993, 2019
StyI	CCWWGG	6 cut(s)	987, 1146, 1328, 1611, 1770, 2088
TaaI	ACNGT	4 cut(s)	332, 530, 1470, 2074
TaiI	ACGT	1 cut(s)	2193
TaqI	TCGA	5 cut(s)	195, 480, 1085, 1674, 1896
TaqII	GACCGA	1 cut(s)	449
TatI	WGTACW	3 cut(s)	942, 2028, 2037
TfiI	GAWTC	6 cut(s)	799, 844, 1268, 1295, 1562, 2174
Tru1I	TTAA	9 cut(s)	107, 137, 180, 351, 378, 1607, 2169, 2184, 2236
Tru9I	TTAA	9 cut(s)	107, 137, 180, 351, 378, 1607, 2169, 2184, 2236
TscAI	CASTG	4 cut(s)	409, 1203, 1666, 2079
TseFI	GTSAC	5 cut(s)	559, 1301, 1470, 1531, 2006
TseI	GCWGC	6 cut(s)	129, 242, 464, 1523, 2102, 2228
Tsp45I	GTSAC	5 cut(s)	559, 1301, 1470, 1531, 2006
TspDTI	ATGAA	9 cut(s)	241, 412, 575, 615, 717, 799, 836, 908, 1852
TspMI	CCCGGG	1 cut(s)	1993
TspRI	CASTG	4 cut(s)	409, 1203, 1666, 2079
Van91I	CCANNNNNTGG	1 cut(s)	1152
Vha464I	CTTAAG	1 cut(s)	377
VpaK11BI	GGWCC	1 cut(s)	2061
XapI	RAATTY	3 cut(s)	337, 806, 993
XbaI	TCTAGA	1 cut(s)	544
XceI	RCATGY	2 cut(s)	539, 555
XcmI	CCANNNNNNNNNTGG	2 cut(s)	290, 2071
XmaI	CCCGGG	1 cut(s)	1993
XmiI	GTMKAC	3 cut(s)	480, 606, 1896
XmnI	GAANNNNTTC	5 cut(s)	912, 1581, 1782, 2147, 2160
XspI	CTAG	3 cut(s)	545, 1299, 1643
Zsp2I	ATGCAT	2 cut(s)	6, 2131

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.