Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0002188

Physical Location & Seq

Genomic Coordinates Forward (+)

12261217 .. 12264376

Gene Structure 3160 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1154300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2268 bp

ATGCTGGACTATACAGGAGTCGTTTGGATTAATATGGATACTGAGTATAAACCGAAGAACATCCTCCTTACTGGAGCTGCTGGCTTCATTGCTTCCCATGTTTGCAACCGGCTTATCCGGAACTATCCTGACTACAACATTGTGGTTCTTGACAAGCTTGATTACTGTTCAAATTTGAAAAACCTTCATCCTTCAATGTCATCCCCCAACTTCAAGTTTATCAAGGGAGACAAAGGCAGTGCTGATTTTGTCAACTTCATCCTTTTGACCGAAAACATTGATACAATAATGCACTTTGCAGCCCAGACCCATGTCGACAACTCTTTTGGTAACAGCTTTGGGTTCACCAAAAACAACATCTATGGTACACATGTTCTTCTAGAAGCATGCAAAGTCACTGGTCAAATCAAAAGATTCGTTCATGTAAGCACAGACGAAGTCTATGGAGAGACAAATGAGGGTGCTGTGGTGGGAAATCATGAGGCTTCCCAGCTTCTTCCGACAAACCCCTACTCTGCAACTAAAGCTGGAGCTGAGATGCTTGTTATGGCATATGGCCGTTCATATGGATTGCCTGTGATAACTACCAGAGGGCACAATGTTTATGGCCGCAACCAGTTCCCTGAAAAGATGATTCCAAAATTCATTCTCTTGGCTATGCAAGGGAAGCCTCTTCCGGTTCATGGTTATGGATCCAGTGTCAGAAGTTATCTCTATTGTGAGGATGTAGCAGAGGCATTTGAAGTCATCCTCCATAGAGGTGAGGTAGGCCATGTGTACAACATTGGAACGAAGAAAGAAAGGAGGGTGGTGGATGTGGCCAAGGAAATCTGCCACCTATTTTCTCTGAACCCTGATACTCATGTAAAGTTTGTAAAGAACAGGCCCTTCAATGATCGGAGATATTTCTTGGATGACCAGACGCTGAAAAACATGGGATGGTCGGAAAGGACTTCGTGGAAGGAGGGTTTGAGGAAGACCATGGGATGGTATGTCAAAAATCCCCAATGGTGGGGAGATGTTTCTGGGGCACTGCTCCCTCATCCAAGAATGATCATGGTTCCTGGAATTGAAAGACAATTTTATGGTTCCGAAACTGGCGATTCTGCTTACCGTTTGTCAGCAAGTGATTCTGATCACAGCAAAATGGTTGCTTCAGCTCCAAGGAACATCCCATCCACTGATAAGCCATCTCTGAAGTTTTTGATTTATGGTAAAACAGGGTATATCGGAGGCCTTCTTGGGAAGATTTGTGAGAAGCAAGGGATACCCTATGAGTATGGACGAGGGCGTCTTCAGGAACGGTCACAGCTCATGGCTGATATTCTAAGTGTTAAACCAAACCATGTTTTTAATGCTGCTGGAGTGACGGGCAGACCCCATGCGGATTGGTGTGAATCCCATAAACCAGAAACAATTCGGACCAATGTTGATGGTACATTAACCTTGGCTGATGTCTGTAGAGATCACGGCCTCCAAATGGTGAATTATGGCAGCGGTTGCATTTTCGAGTATGATGATGCCCATCCAGGGCCGGCCCAGGCCCAGTGCAGGAGGGGCAACCGTTCAGGGCCCAAAAAATTTGGGGCACCAAAATTATTAGGGTGGTATATTTACATATATTTTTATAAGAGGCTTTTTTTTTTACGTCGCCCTGGGCCTCAAAAATTTCTGGACCGGCCCTGTGCCCATCCTGCAAGATCAGGAATTGGGTTCAAGGAGGAAGACACACCCAATTTCGCGGGTTCATTTTATTCCAAAACCAAAGCCATGGTTGAAGAGCTTCTGAAGGAGTATGAGAATGTTTGCACTCTTAGAGTTCGAATGCCAATATCATCTGATCTCAGCAACCCACGCAACTTCATCACAAAGATCTCGCGGTACAATAATGTGGTCGACATTCCAAACAGCATGACCATCTTGGACGAGCTTCTGCCCATTTCAGTTGAGATGGCCAAGAGGAACTTGAGGGGCATATGGAACTTCACAAACCCCGGCGTGTTGAGTCACAACGAGATTCTGGAGATGTACAAGAACTACATTGACCCCAGTTTCAAGTGGACTAATTTTACTGTGGAAGAACAAGCCAAGGTTCTCGTTGCAGGAAGAAGTAACTGTGAGGTGGATGCATCTAAGCTGAAGAAAGAGTTCCCTGAACTGCTCCCGGTCAAGGAATCGCTGATTAAATACGTGTTTGAACCCAACAAGAAAACATTTGCAGGTAGGGCAGCAATTGAAGTGTTTCATGAAGTCGACCTCGTTACCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005911 GO:0006139 GO:0006725 GO:0006793 GO:0006807 GO:0006810 GO:0008150 GO:0008152 GO:0009058 GO:0009225 GO:0009226 GO:0009506 GO:0009812 GO:0009813 GO:0009914 GO:0009966 GO:0009987 GO:0010253 GO:0010280 GO:0010315 GO:0010646 GO:0010817 GO:0010928 GO:0016829 GO:0016835 GO:0016836 GO:0018130 GO:0019438 GO:0023051 GO:0030054 GO:0030154 GO:0032502 GO:0033478 GO:0034641 GO:0034654 GO:0042127 GO:0042440 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046148 GO:0046483 GO:0048583 GO:0048869 GO:0050377 GO:0050789 GO:0050794 GO:0051179 GO:0051234 GO:0051552 GO:0051553 GO:0051554 GO:0051555 GO:0055044 GO:0055086 GO:0060918 GO:0065007 GO:0065008 GO:0071704 GO:1901135 GO:1901137 GO:1901360 GO:1901362 GO:1901576 GO:1901615 GO:1901617

KEGG Pathways

Metabolic & Signaling

ko00520 map00520

Pfam Domains

Protein Families

Epimerase GDP_Man_Dehyd

Protein Analysis

756

Amino Acids

85.44

Weight (kDa)

8.89

Isoelectric Point (pI)

33.75

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RmlD_sub_bind	PF04321	20 - 327	2.4e-11	RmlD substrate binding domain
Epimerase	PF01370	21 - 263	1.8e-52	NAD dependent epimerase/dehydratase family
Polysacc_synt_2	PF02719	21 - 293	3.3e-17	Polysaccharide biosynthesis protein
GDP_Man_Dehyd	PF16363	22 - 327	4.5e-59	GDP-mannose 4,6 dehydratase
3Beta_HSD	PF01073	23 - 246	1.7e-19	3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_4	PF07993	23 - 205	1e-17	Male sterility protein
RmlD_sub_bind	PF04321	400 - 506	4.8e-07	RmlD substrate binding domain
Epimerase	PF01370	405 - 510	1.2e-07	NAD dependent epimerase/dehydratase family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready

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Orthologous Genes (Group: OG0002188)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G53500 AT3G14790 AT3G14790
fragaria_vesca	FvH4_2g36650 FvH4_2g36650 FvH4_6g38080 FvH4_6g38080 FvH4_6g38080 FvH4_6g38080 FvH4_6g38080
malus_domestica	MD09G1154300.v1.1 MD09G1154700.v1.1 MD15G1106300.v1.1 MD17G1140800.v1.1
prunus_persica	Prupe.1G458500_v2.0.a1 Prupe.3G170800_v2.0.a1
pyrus_communis	pycom09g07360 pycom15g09690 pycom17g13400
rosa_chinensis	RchiOBHm_Chr2g0151131 RchiOBHm_Chr6g0305141
rosa_laevigata	RLG00000010914 RLG00000020533
rosa_multiflora	Rmu_sc0000061.1_g000019 Rmu_sc0032179.1_g000002
rosa_roxburghii	Rroxscaffold_2G00096710 Rroxscaffold_7G00163120
rosa_rugosa	Rorug02G0425000 Rorug02G0425100 Rorug06G0338000 Rorug06G0338100 Rorug06G0338200 Rorug06G0338300
rosa_samantha	Rh2AG485600 Rh2BG497500 Rh2CG471400 Rh2DG508500 Rh6AG449700 Rh6BG451700 Rh6CG463100 Rh6DG450100
rosa_wichuraiana	Rw2G039770 Rw6G039160

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1629
AasI	GACNNNNNNGTC	1 cut(s)	1290
Acc36I	ACCTGC	1 cut(s)	2210
AccB1I	GGYRCC	1 cut(s)	1588
AccB7I	CCANNNNNTGG	1 cut(s)	987
AccI	GTMKAC	3 cut(s)	315, 1896, 2253
AccII	CGCG	2 cut(s)	1742, 1879
AccIII	TCCGGA	1 cut(s)	117
AciI	CCGC	5 cut(s)	610, 1385, 1497, 1742, 1879
AclWI	GGATC	2 cut(s)	687, 700
AcoI	YGGCCR	4 cut(s)	556, 607, 819, 1953
AcsI	RAATTY	4 cut(s)	172, 641, 1580, 1667
AcuI	CTGAAG	5 cut(s)	1140, 1217, 1280, 1808, 2159
AcyI	GRCGYC	1 cut(s)	1291
AfaI	GTAC	5 cut(s)	367, 779, 1438, 1883, 2030
AfiI	CCNNNNNNNGG	8 cut(s)	683, 987, 1011, 1012, 1480, 1530, 1551, 2170
AflIII	ACRYGT	2 cut(s)	370, 2190
AjnI	CCWGG	4 cut(s)	1063, 1528, 1539, 1654
AloI	GAACNNNNNNTCC	2 cut(s)	1160, 1192
Alw26I	GTCTC	2 cut(s)	222, 443
AlwI	GGATC	2 cut(s)	687, 700
AlwNI	CAGNNNCTG	1 cut(s)	925
Aor13HI	TCCGGA	1 cut(s)	117
ApaI	GGGCCC	1 cut(s)	1575
ApeKI	GCWGC	5 cut(s)	77, 299, 1358, 1494, 2228
ApoI	RAATTY	4 cut(s)	172, 641, 1580, 1667
ArsI	GACNNNNNNTTYG	2 cut(s)	308, 340
AseI	ATTAAT	1 cut(s)	30
Asp700I	GAANNNNTTC	4 cut(s)	1416, 1782, 2147, 2241
AsuC2I	CCSGG	2 cut(s)	1995, 2165
AsuHPI	GGTGA	3 cut(s)	337, 773, 1495
AsuII	TTCGAA	1 cut(s)	1822
AvaII	GGWCC	2 cut(s)	1422, 1675
BaeGI	GKGCMC	5 cut(s)	597, 1033, 1575, 1591, 1690
BaeI	ACNNNNGTAYC	4 cut(s)	357, 390, 849, 882
BalI	TGGCCA	2 cut(s)	821, 1955
BamHI	GGATCC	1 cut(s)	692
BanI	GGYRCC	1 cut(s)	1588
BanII	GRGCYC	1 cut(s)	1575
BbsI	GAAGAC	3 cut(s)	983, 1286, 1731
BbvI	GCAGC	5 cut(s)	64, 311, 1345, 1506, 2240
BccI	CCATC	9 cut(s)	933, 981, 1183, 1198, 1427, 1533, 1698, 1925, 1945
BceAI	ACGGC	2 cut(s)	543, 1486
BciT130I	CCWGG	4 cut(s)	1065, 1530, 1541, 1656
BciVI	GTATCC	2 cut(s)	31, 1260
BclI	TGATCA	2 cut(s)	1053, 1135
BcnI	CCSGG	2 cut(s)	1995, 2165
BcoDI	GTCTC	2 cut(s)	222, 443
BfaI	CTAG	2 cut(s)	380, 2266
BfmI	CTRYAG	1 cut(s)	1459
BfuAI	ACCTGC	1 cut(s)	2210
BfuI	GTATCC	2 cut(s)	31, 1260
BglII	AGATCT	1 cut(s)	1872
BisI	GCNGC	6 cut(s)	78, 300, 610, 1359, 1495, 2229
BlsI	GCNGC	6 cut(s)	79, 301, 611, 1360, 1496, 2230
Bme1390I	CCNGG	6 cut(s)	1065, 1530, 1541, 1656, 1995, 2165
Bme18I	GGWCC	2 cut(s)	1422, 1675
BmiI	GGNNCC	5 cut(s)	694, 1062, 1090, 1573, 1590
BmrFI	CCNGG	6 cut(s)	1065, 1530, 1541, 1656, 1995, 2165
BmrI	ACTGGG	2 cut(s)	1540, 2043
BmsI	GCATC	4 cut(s)	528, 1510, 2116, 2138
BmuI	ACTGGG	2 cut(s)	1540, 2043
BoxI	GACNNNNGTC	1 cut(s)	311
BpiI	GAAGAC	3 cut(s)	983, 1286, 1731
BpmI	CTGGAG	4 cut(s)	93, 549, 1383, 2042
Bpu14I	TTCGAA	1 cut(s)	1822
BpuEI	CTTGAG	1 cut(s)	1987
BpuMI	CCSGG	2 cut(s)	1995, 2165
BsaAI	YACGTR	1 cut(s)	2191
BsaBI	GATNNNNATC	2 cut(s)	1134, 1524
BsaHI	GRCGYC	1 cut(s)	1291
BsaWI	WCCGGW	2 cut(s)	117, 676
Bsc4I	CCNNNNNNNGG	8 cut(s)	683, 987, 1011, 1012, 1480, 1530, 1551, 2170
Bse118I	RCCGGY	3 cut(s)	108, 1534, 1677
Bse1I	ACTGG	7 cut(s)	76, 403, 616, 696, 1102, 1546, 2049
Bse3DI	GCAATG	1 cut(s)	87
Bse8I	GATNNNNATC	2 cut(s)	1134, 1524
BseAI	TCCGGA	1 cut(s)	117
BseBI	CCWGG	4 cut(s)	1065, 1530, 1541, 1656
BseJI	GATNNNNATC	2 cut(s)	1134, 1524
BseLI	CCNNNNNNNGG	8 cut(s)	683, 987, 1011, 1012, 1480, 1530, 1551, 2170
BseMI	GCAATG	1 cut(s)	87
BseMII	CTCAG	3 cut(s)	33, 525, 1858
BseNI	ACTGG	7 cut(s)	76, 403, 616, 696, 1102, 1546, 2049
BseSI	GKGCMC	5 cut(s)	597, 1033, 1575, 1591, 1690
BseXI	GCAGC	5 cut(s)	64, 311, 1345, 1506, 2240
BseYI	CCCAGC	1 cut(s)	489
BsgI	GTGCAG	1 cut(s)	1570
Bsh1236I	CGCG	2 cut(s)	1742, 1879
BshNI	GGYRCC	1 cut(s)	1588
BsiSI	CCGG	7 cut(s)	109, 118, 677, 1535, 1678, 1995, 2165
BslI	CCNNNNNNNGG	8 cut(s)	683, 987, 1011, 1012, 1480, 1530, 1551, 2170
BsmAI	GTCTC	2 cut(s)	222, 443
BsmI	GAATGC	1 cut(s)	1830
Bsp119I	TTCGAA	1 cut(s)	1822
Bsp120I	GGGCCC	1 cut(s)	1571
Bsp1286I	GDGCHC	5 cut(s)	597, 1033, 1575, 1591, 1690
Bsp13I	TCCGGA	1 cut(s)	117
Bsp1407I	TGTACA	2 cut(s)	777, 2028
Bsp143I	GATC	8 cut(s)	692, 895, 1053, 1135, 1465, 1700, 1840, 1872
Bsp19I	CCATGG	2 cut(s)	981, 1770
BspACI	CCGC	5 cut(s)	610, 1385, 1497, 1742, 1879
BspCNI	CTCAG	3 cut(s)	34, 526, 1857
BspEI	TCCGGA	1 cut(s)	117
BspFNI	CGCG	2 cut(s)	1742, 1879
BspHI	TCATGA	2 cut(s)	478, 2245
BspLI	GGNNCC	5 cut(s)	694, 1062, 1090, 1573, 1590
BspMI	ACCTGC	1 cut(s)	2210
BspPI	GGATC	2 cut(s)	687, 700
BspQI	GCTCTTC	1 cut(s)	1773
BspT104I	TTCGAA	1 cut(s)	1822
BspT107I	GGYRCC	1 cut(s)	1588
BsrDI	GCAATG	1 cut(s)	87
BsrFI	RCCGGY	3 cut(s)	108, 1534, 1677
BsrGI	TGTACA	2 cut(s)	777, 2028
BsrI	ACTGG	7 cut(s)	76, 403, 616, 696, 1102, 1546, 2049
BssAI	RCCGGY	3 cut(s)	108, 1534, 1677
BssMI	GATC	8 cut(s)	692, 895, 1053, 1135, 1465, 1700, 1840, 1872
BssNI	GRCGYC	1 cut(s)	1291
BssT1I	CCWWGG	6 cut(s)	822, 981, 1163, 1446, 1770, 2088
Bst2UI	CCWGG	4 cut(s)	1065, 1530, 1541, 1656
Bst4CI	ACNGT	6 cut(s)	167, 1115, 1305, 1565, 2074, 2117
Bst6I	CTCTTC	2 cut(s)	678, 1773
BstACI	GRCGYC	1 cut(s)	1291
BstAPI	GCANNNNNTGC	1 cut(s)	1500
BstAUI	TGTACA	2 cut(s)	777, 2028
BstBAI	YACGTR	1 cut(s)	2191
BstBI	TTCGAA	1 cut(s)	1822
BstC8I	GCNNGC	3 cut(s)	82, 388, 1536
BstDEI	CTNAG	6 cut(s)	42, 534, 1328, 1814, 1844, 2133
BstDSI	CCRYGG	2 cut(s)	981, 1770
BstFNI	CGCG	2 cut(s)	1742, 1879
BstKTI	GATC	8 cut(s)	695, 898, 1056, 1138, 1468, 1703, 1843, 1875
BstMAI	GTCTC	2 cut(s)	222, 443
BstMBI	GATC	8 cut(s)	692, 895, 1053, 1135, 1465, 1700, 1840, 1872
BstMWI	GCNNNNNNNGC	7 cut(s)	524, 667, 1500, 1557, 1694, 1854, 2225
BstNI	CCWGG	4 cut(s)	1065, 1530, 1541, 1656
BstNSI	RCATGY	2 cut(s)	374, 390
BstPAI	GACNNNNGTC	1 cut(s)	311
BstSCI	CCNGG	6 cut(s)	1063, 1528, 1539, 1654, 1993, 2163
BstSFI	CTRYAG	1 cut(s)	1459
BstSLI	GKGCMC	5 cut(s)	597, 1033, 1575, 1591, 1690
BstUI	CGCG	2 cut(s)	1742, 1879
BstV1I	GCAGC	5 cut(s)	64, 311, 1345, 1506, 2240
BstV2I	GAAGAC	3 cut(s)	983, 1286, 1731
BstX2I	RGATCY	2 cut(s)	692, 1872
BstXI	CCANNNNNNTGG	1 cut(s)	1771
BstYI	RGATCY	2 cut(s)	692, 1872
BsuI	GTATCC	2 cut(s)	31, 1260
BtgI	CCRYGG	2 cut(s)	981, 1770
BtsI	GCAGTG	2 cut(s)	244, 1031
BtsIMutI	CAGTG	6 cut(s)	244, 396, 703, 1031, 1179, 1553
BveI	ACCTGC	1 cut(s)	2210
Cac8I	GCNNGC	3 cut(s)	82, 388, 1536
CaiI	CAGNNNCTG	1 cut(s)	925
CciI	TCATGA	2 cut(s)	478, 2245
Cfr10I	RCCGGY	3 cut(s)	108, 1534, 1677
CseI	GACGC	2 cut(s)	931, 1280
Csp6I	GTAC	5 cut(s)	366, 778, 1437, 1882, 2029
CviQI	GTAC	5 cut(s)	366, 778, 1437, 1882, 2029
DdeI	CTNAG	6 cut(s)	42, 534, 1328, 1814, 1844, 2133
DpnI	GATC	8 cut(s)	694, 897, 1055, 1137, 1467, 1702, 1842, 1874
DpnII	GATC	8 cut(s)	692, 895, 1053, 1135, 1465, 1700, 1840, 1872
DrdI	GACNNNNNNGTC	1 cut(s)	1290
DseDI	GACNNNNNNGTC	1 cut(s)	1290
EaeI	YGGCCR	4 cut(s)	556, 607, 819, 1953
Eam1104I	CTCTTC	2 cut(s)	678, 1773
EarI	CTCTTC	2 cut(s)	678, 1773
Eco130I	CCWWGG	6 cut(s)	822, 981, 1163, 1446, 1770, 2088
Eco147I	AGGCCT	1 cut(s)	1236
Eco24I	GRGCYC	1 cut(s)	1575
Eco47I	GGWCC	2 cut(s)	1422, 1675
Eco57I	CTGAAG	5 cut(s)	1140, 1217, 1280, 1808, 2159
EcoO109I	RGGNCCY	2 cut(s)	885, 1571
EcoRII	CCWGG	4 cut(s)	1063, 1528, 1539, 1654
EcoT14I	CCWWGG	6 cut(s)	822, 981, 1163, 1446, 1770, 2088
EcoT22I	ATGCAT	1 cut(s)	2131
EcoT38I	GRGCYC	1 cut(s)	1575
ErhI	CCWWGG	6 cut(s)	822, 981, 1163, 1446, 1770, 2088
FalI	AAGNNNNNCTT	2 cut(s)	1949, 1981
FauI	CCCGC	1 cut(s)	1735
FauNDI	CATATG	3 cut(s)	553, 565, 1976
FbaI	TGATCA	2 cut(s)	1053, 1135
FblI	GTMKAC	3 cut(s)	315, 1896, 2253
Fnu4HI	GCNGC	6 cut(s)	78, 300, 610, 1359, 1495, 2229
FriOI	GRGCYC	1 cut(s)	1575
FseI	GGCCGGCC	1 cut(s)	1538
Fsp4HI	GCNGC	6 cut(s)	78, 300, 610, 1359, 1495, 2229
FspBI	CTAG	2 cut(s)	380, 2266
GluI	GCNGC	6 cut(s)	78, 300, 610, 1359, 1495, 2229
GsaI	CCCAGC	1 cut(s)	493
GsuI	CTGGAG	4 cut(s)	93, 549, 1383, 2042
HapII	CCGG	7 cut(s)	109, 118, 677, 1535, 1678, 1995, 2165
HgaI	GACGC	2 cut(s)	931, 1280
Hin1I	GRCGYC	1 cut(s)	1291
HincII	GTYRAC	4 cut(s)	253, 316, 1897, 2254
HindII	GTYRAC	4 cut(s)	253, 316, 1897, 2254
HindIII	AAGCTT	1 cut(s)	155
HinfI	GANTC	9 cut(s)	18, 414, 634, 1103, 1130, 1397, 2005, 2017, 2174
HpaII	CCGG	7 cut(s)	109, 118, 677, 1535, 1678, 1995, 2165
HphI	GGTGA	3 cut(s)	337, 773, 1495
Hpy166II	GTNNAC	8 cut(s)	253, 316, 345, 368, 778, 1897, 2061, 2254
Hpy8I	GTNNAC	8 cut(s)	253, 316, 345, 368, 778, 1897, 2061, 2254
Hpy99I	CGWCG	1 cut(s)	1653
HpyAV	CCTTC	6 cut(s)	194, 201, 898, 955, 1247, 1783
HpyCH4III	ACNGT	6 cut(s)	167, 1115, 1305, 1565, 2074, 2117
HpyCH4IV	ACGT	2 cut(s)	1648, 2190
HpyF10VI	GCNNNNNNNGC	7 cut(s)	524, 667, 1500, 1557, 1694, 1854, 2225
HpyF3I	CTNAG	6 cut(s)	42, 534, 1328, 1814, 1844, 2133
HpySE526I	ACGT	2 cut(s)	1648, 2190
Hsp92I	GRCGYC	1 cut(s)	1291
Kpn2I	TCCGGA	1 cut(s)	117
KroI	GCCGGC	1 cut(s)	1534
KroNI	GCCGGC	1 cut(s)	1536
Ksp22I	TGATCA	2 cut(s)	1053, 1135
Kzo9I	GATC	8 cut(s)	692, 895, 1053, 1135, 1465, 1700, 1840, 1872
LguI	GCTCTTC	1 cut(s)	1773
LmnI	GCTCC	5 cut(s)	74, 530, 1041, 1165, 2166
Lsp1109I	GCAGC	5 cut(s)	64, 311, 1345, 1506, 2240
LweI	GCATC	4 cut(s)	528, 1510, 2116, 2138
MaeI	CTAG	2 cut(s)	380, 2266
MaeII	ACGT	2 cut(s)	1648, 2190
MaeIII	GTNAC	7 cut(s)	329, 394, 1305, 1366, 2006, 2111, 2260
MalI	GATC	8 cut(s)	694, 897, 1055, 1137, 1467, 1702, 1842, 1874
MboI	GATC	8 cut(s)	692, 895, 1053, 1135, 1465, 1700, 1840, 1872
MfeI	CAATTG	1 cut(s)	2232
MflI	RGATCY	2 cut(s)	692, 1872
MhlI	GDGCHC	5 cut(s)	597, 1033, 1575, 1591, 1690
MlsI	TGGCCA	2 cut(s)	821, 1955
MluNI	TGGCCA	2 cut(s)	821, 1955
MlyI	GAGTC	2 cut(s)	27, 2014
MmeI	TCCRAC	2 cut(s)	524, 924
Mox20I	TGGCCA	2 cut(s)	821, 1955
Mph1103I	ATGCAT	1 cut(s)	2131
MroI	TCCGGA	1 cut(s)	117
MroNI	GCCGGC	1 cut(s)	1534
MroXI	GAANNNNTTC	4 cut(s)	1416, 1782, 2147, 2241
MscI	TGGCCA	2 cut(s)	821, 1955
MseI	TTAA	5 cut(s)	30, 1335, 1353, 1442, 2184
MslI	CAYNNNNRTG	2 cut(s)	687, 759
Msp20I	TGGCCA	2 cut(s)	821, 1955
MspA1I	CMGCKG	1 cut(s)	1497
MspI	CCGG	7 cut(s)	109, 118, 677, 1535, 1678, 1995, 2165
MspR9I	CCNGG	6 cut(s)	1065, 1530, 1541, 1656, 1995, 2165
MunI	CAATTG	1 cut(s)	2232
Mva1269I	GAATGC	1 cut(s)	1830
MvaI	CCWGG	4 cut(s)	1065, 1530, 1541, 1656
MvnI	CGCG	2 cut(s)	1742, 1879
MwoI	GCNNNNNNNGC	7 cut(s)	524, 667, 1500, 1557, 1694, 1854, 2225
NaeI	GCCGGC	1 cut(s)	1536
NciI	CCSGG	2 cut(s)	1995, 2165
NcoI	CCATGG	2 cut(s)	981, 1770
NdeI	CATATG	3 cut(s)	553, 565, 1976
NdeII	GATC	8 cut(s)	692, 895, 1053, 1135, 1465, 1700, 1840, 1872
NgoMIV	GCCGGC	1 cut(s)	1534
NlaIV	GGNNCC	5 cut(s)	694, 1062, 1090, 1573, 1590
NmuCI	GTSAC	4 cut(s)	394, 1305, 1366, 2006
NsiI	ATGCAT	1 cut(s)	2131
NspI	RCATGY	2 cut(s)	374, 390
NspV	TTCGAA	1 cut(s)	1822
PaeI	GCATGC	1 cut(s)	390
PagI	TCATGA	2 cut(s)	478, 2245
PasI	CCCWGGG	1 cut(s)	1655
PceI	AGGCCT	1 cut(s)	1236
PciI	ACATGT	1 cut(s)	370
PciSI	GCTCTTC	1 cut(s)	1773
PctI	GAATGC	1 cut(s)	1830
PdiI	GCCGGC	1 cut(s)	1536
PdmI	GAANNNNTTC	4 cut(s)	1416, 1782, 2147, 2241
PfeI	GAWTC	7 cut(s)	414, 634, 1103, 1130, 1397, 2017, 2174
PflFI	GACNNNGTC	1 cut(s)	437
PflMI	CCANNNNNTGG	1 cut(s)	987
PfoI	TCCNGGA	1 cut(s)	1063
PkrI	GCNGC	6 cut(s)	79, 301, 611, 1360, 1496, 2230
PleI	GAGTC	2 cut(s)	26, 2013
PpsI	GAGTC	2 cut(s)	26, 2013
Ppu21I	YACGTR	1 cut(s)	2191
PscI	ACATGT	1 cut(s)	370
PshAI	GACNNNNGTC	1 cut(s)	311
PshBI	ATTAAT	1 cut(s)	30
PsiI	TTATAA	1 cut(s)	1629
Psp6I	CCWGG	4 cut(s)	1063, 1528, 1539, 1654
PspFI	CCCAGC	1 cut(s)	489
PspGI	CCWGG	4 cut(s)	1063, 1528, 1539, 1654
PspN4I	GGNNCC	5 cut(s)	694, 1062, 1090, 1573, 1590
PspOMI	GGGCCC	1 cut(s)	1571
PstNI	CAGNNNCTG	1 cut(s)	925
PsuI	RGATCY	2 cut(s)	692, 1872
PsyI	GACNNNGTC	1 cut(s)	437
RigI	GGCCGGCC	1 cut(s)	1538
RsaI	GTAC	5 cut(s)	367, 779, 1438, 1883, 2030
RsaNI	GTAC	5 cut(s)	366, 778, 1437, 1882, 2029
RseI	CAYNNNNRTG	2 cut(s)	687, 759
SalI	GTCGAC	3 cut(s)	314, 1895, 2252
SapI	GCTCTTC	1 cut(s)	1773
SaqAI	TTAA	5 cut(s)	30, 1335, 1353, 1442, 2184
SatI	GCNGC	6 cut(s)	78, 300, 610, 1359, 1495, 2229
Sau3AI	GATC	8 cut(s)	692, 895, 1053, 1135, 1465, 1700, 1840, 1872
SchI	GAGTC	2 cut(s)	27, 2014
ScrFI	CCNGG	6 cut(s)	1065, 1530, 1541, 1656, 1995, 2165
SduI	GDGCHC	5 cut(s)	597, 1033, 1575, 1591, 1690
SfaNI	GCATC	4 cut(s)	528, 1510, 2116, 2138
SfcI	CTRYAG	1 cut(s)	1459
SfuI	TTCGAA	1 cut(s)	1822
SinI	GGWCC	2 cut(s)	1422, 1675
SmiMI	CAYNNNNRTG	2 cut(s)	687, 759
SmlI	CTYRAG	1 cut(s)	1966
SmoI	CTYRAG	1 cut(s)	1966
SphI	GCATGC	1 cut(s)	390
SseBI	AGGCCT	1 cut(s)	1236
SsiI	CCGC	5 cut(s)	610, 1385, 1497, 1742, 1879
SspMI	CTAG	2 cut(s)	380, 2266
StuI	AGGCCT	1 cut(s)	1236
StyD4I	CCNGG	6 cut(s)	1063, 1528, 1539, 1654, 1993, 2163
StyI	CCWWGG	6 cut(s)	822, 981, 1163, 1446, 1770, 2088
TaaI	ACNGT	6 cut(s)	167, 1115, 1305, 1565, 2074, 2117
TaiI	ACGT	2 cut(s)	1651, 2193
TaqI	TCGA	5 cut(s)	315, 1509, 1822, 1896, 2253
TaqII	GACCGA	1 cut(s)	284
TatI	WGTACW	2 cut(s)	777, 2028
TauI	GCSGC	1 cut(s)	612
TfiI	GAWTC	7 cut(s)	414, 634, 1103, 1130, 1397, 2017, 2174
Tru1I	TTAA	5 cut(s)	30, 1335, 1353, 1442, 2184
Tru9I	TTAA	5 cut(s)	30, 1335, 1353, 1442, 2184
TscAI	CASTG	6 cut(s)	244, 403, 703, 1038, 1186, 1553
TseFI	GTSAC	4 cut(s)	394, 1305, 1366, 2006
TseI	GCWGC	5 cut(s)	77, 299, 1358, 1494, 2228
Tsp45I	GTSAC	4 cut(s)	394, 1305, 1366, 2006
TspRI	CASTG	6 cut(s)	244, 403, 703, 1038, 1186, 1553
Tth111I	GACNNNGTC	1 cut(s)	437
Van91I	CCANNNNNTGG	1 cut(s)	987
VpaK11BI	GGWCC	2 cut(s)	1422, 1675
VspI	ATTAAT	1 cut(s)	30
XapI	RAATTY	4 cut(s)	172, 641, 1580, 1667
XbaI	TCTAGA	1 cut(s)	379
XceI	RCATGY	2 cut(s)	374, 390
XmiI	GTMKAC	3 cut(s)	315, 1896, 2253
XmnI	GAANNNNTTC	4 cut(s)	1416, 1782, 2147, 2241
XspI	CTAG	2 cut(s)	380, 2266
Zsp2I	ATGCAT	1 cut(s)	2131

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.