Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0002188

Physical Location & Seq

Genomic Coordinates Forward (+)

18044305 .. 18048385

Gene Structure 4081 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00096710.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2040 bp

ATGGGTACCGCCTATAAACCGAAGAACATCCTCATTACTGGAGCTGCTGGCTTCATTGCATCCCATGTTTGCAATCGGCTTATCAGGAACTACCCTGAATACAAGATTGTCGTCCTTGACAAGCTTGATTACTGTTCAAATTTGAAGAACCTTCATCCCTCAAGGTCATCCCCAAACTTCAAATTTATCAAGGGAGACATTGGAAGCGCTGACCTTGTCAACTTCATCCTTCTCACCGAGTCCATTGATACAATAATGCACTTTGCTGCCCAGACCCATGTTGACAACTCATTTGGTAACAGCTTTGAGTTCACTAAAAACAACATATATGGCACGCATGTTCTTCTAGAAGCATGCAAAGTCACTGGCCAAATCAAAAGGTTCATCCATGTTAGCACAGATGAAGTTTATGGGGAGACAGATGAGGATGCTGTGGTGGGAAATCATGAGGCTTCTCAGCTTCTTCCCACAAACCCCTACTCTGCGACTAAAGCTGGAGCAGAGATGCTTGTTATGGCATATGGACGTTCATATGGGCTGCCTGTGATAACTACCAGGGGAAACAATGTTTATGGCCCCAATCAGTTCCCTGAAAAGATGATTCCAAAGTTCATTCTCTTGGCTATGAAAGGGAAGACTCTTCCGATTCACGGTGATGGATCAAATGTCAGGAGTTATCTCTATTGTGAGGATGTAGCCGAGGCATTTGAGGTCATTCTCCATAAGGGTGAGGTAGGCCATGTCTACAACATTGGGACGAAGAAAGAGAGGAGGGTAGTTGATGTCGCTAGGGAAATTTGCCAACTGTTCTCATTGAACCCAGATACATATATAAACTTTGTTGAGAATAGACCTTTTAATGATCAGAGGTATTTTCTGGATGACCAAAAGCTTAAAAACCTGGGATGGTCTGAAAGTACTTCATGGGAGGAAGGTTTGAGGAAGACAATGGAATGGTATGTCAAGAATCCTCATTGGTGGGGAGATGTTTCGGGAGCACTGCTCCCTCATCCAAGAATGCTCATGGTTCCTGGAATTGAAAGAAACTTTGATGGGCCTGATACAAGCAATTCCGATTCCCCTGTTCCAGCAACTGATTCTAGTCAAAGCCAAGTGGTTGTTCCAGCTCCTAGGGGCATTCCATCTACTCAGAAGCCATCTCTGAAGTTCCTGATTTATGGGAGAACAGGGTGGATTGGAGGCCTTCTTGGGAAGATTTGTGAGAAACAAGGGATACCATTTGAGTATGGACGAGGGCGGCTCCAGGAACGTTCTCAGCTCTTGGCTGATATTCAGAGGGTTAAGCCAACCCATGTTTTCAATGCTGCTGGAGTGACTGGCAGACCCAATGTGGATTGGTGTGAATCTCATAAACCAGAAACAATTCGGACCAATGTTGTGGGTACATTGACCTTGGCTGATGTCTGCAGAGAGCACAACCTCCTAATGATGAATTATGCTACTGGTTGTATCTTCGAGTACGATGCAGCTCATCCATCAAGATCAGGAATTGGGTTCAAGGAGGAAGATACACCAAATTTCACCGGTTCATTTTACTCCAAAACCAAAGCCATGGTGGAAGAGCTTTTGAAAGAATATGACAATGTCTGCACTCTTAGAGTTCGAATGCCTATATCATCTGATCTCAGCAATCCACGCAACTTCATCACAAAGATCTCCAAGTACAACAAAGTGGTTGATATTCCAAACAGCATGACTATCTTGGATGAACTTCTACCGATTTCAGTTGAGATGGCCAAGAGGAACTTGAAGGGCATATGGAACTTCACAAACCCTGGTGTTGTGAGTCACAATGAGATCCTGGAGATGTACAAGAAGTACATAGACCCCAGTTTCAAGTGGGTTAATTTTACATTGGAAGAACAAGCCAAGGTTATTGTGGCCCCAAGAAGTAACAATGAGATGGATGCATCTAAGTTGAAGAAAGAGTTCCCCGAACTATTGCCAATCAAGGAGTCGCTGATTAAATACGTCTTTGAGCCCAACAAGAAAACATTTGCTGGTGCGGCAGCAAATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005911 GO:0006139 GO:0006725 GO:0006793 GO:0006807 GO:0006810 GO:0008150 GO:0008152 GO:0009058 GO:0009225 GO:0009226 GO:0009506 GO:0009812 GO:0009813 GO:0009914 GO:0009966 GO:0009987 GO:0010253 GO:0010280 GO:0010315 GO:0010646 GO:0010817 GO:0010928 GO:0016829 GO:0016835 GO:0016836 GO:0018130 GO:0019438 GO:0023051 GO:0030054 GO:0030154 GO:0032502 GO:0033478 GO:0034641 GO:0034654 GO:0042127 GO:0042440 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046148 GO:0046483 GO:0048583 GO:0048869 GO:0050377 GO:0050789 GO:0050794 GO:0051179 GO:0051234 GO:0051552 GO:0051553 GO:0051554 GO:0051555 GO:0055044 GO:0055086 GO:0060918 GO:0065007 GO:0065008 GO:0071704 GO:1901135 GO:1901137 GO:1901360 GO:1901362 GO:1901576 GO:1901615 GO:1901617

KEGG Pathways

Metabolic & Signaling

ko00520 map00520

Pfam Domains

Protein Families

Epimerase GDP_Man_Dehyd

Protein Analysis

679

Amino Acids

76.43

Weight (kDa)

7.59

Isoelectric Point (pI)

33.04

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RmlD_sub_bind	PF04321	9 - 318	1.2e-13	RmlD substrate binding domain
Epimerase	PF01370	10 - 252	6.9e-57	NAD dependent epimerase/dehydratase family
Polysacc_synt_2	PF02719	10 - 281	4.3e-20	Polysaccharide biosynthesis protein
GDP_Man_Dehyd	PF16363	11 - 316	1e-65	GDP-mannose 4,6 dehydratase
3Beta_HSD	PF01073	11 - 236	3.7e-24	3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_4	PF07993	12 - 194	6.8e-19	Male sterility protein
RmlD_sub_bind	PF04321	389 - 558	3.9e-11	RmlD substrate binding domain
Epimerase	PF01370	418 - 553	5.7e-11	NAD dependent epimerase/dehydratase family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0002188)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G53500 AT3G14790 AT3G14790
fragaria_vesca	FvH4_2g36650 FvH4_2g36650 FvH4_6g38080 FvH4_6g38080 FvH4_6g38080 FvH4_6g38080 FvH4_6g38080
malus_domestica	MD09G1154300.v1.1 MD09G1154700.v1.1 MD15G1106300.v1.1 MD17G1140800.v1.1
prunus_persica	Prupe.1G458500_v2.0.a1 Prupe.3G170800_v2.0.a1
pyrus_communis	pycom09g07360 pycom15g09690 pycom17g13400
rosa_chinensis	RchiOBHm_Chr2g0151131 RchiOBHm_Chr6g0305141
rosa_laevigata	RLG00000010914 RLG00000020533
rosa_multiflora	Rmu_sc0000061.1_g000019 Rmu_sc0032179.1_g000002
rosa_roxburghii	Rroxscaffold_2G00096710 Rroxscaffold_7G00163120
rosa_rugosa	Rorug02G0425000 Rorug02G0425100 Rorug06G0338000 Rorug06G0338100 Rorug06G0338200 Rorug06G0338300
rosa_samantha	Rh2AG485600 Rh2BG497500 Rh2CG471400 Rh2DG508500 Rh6AG449700 Rh6BG451700 Rh6CG463100 Rh6DG450100
rosa_wichuraiana	Rw2G039770 Rw6G039160

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	5
AccB1I	GGYRCC	1 cut(s)	5
AccI	GTMKAC	1 cut(s)	744
AciI	CCGC	3 cut(s)	9, 1258, 2027
AclI	AACGTT	1 cut(s)	1270
AclWI	GGATC	2 cut(s)	667, 1813
AcoI	YGGCCR	2 cut(s)	367, 1755
AcsI	RAATTY	4 cut(s)	139, 182, 795, 1537
AcuI	CTGAAG	1 cut(s)	1184
AfaI	GTAC	7 cut(s)	7, 919, 1405, 1481, 1685, 1832, 1841
AfeI	AGCGCT	1 cut(s)	208
AfiI	CCNNNNNNNGG	1 cut(s)	650
AgeI	ACCGGT	1 cut(s)	1544
AjnI	CCWGG	6 cut(s)	554, 900, 1030, 1263, 1795, 1821
AjuI	GAANNNNNNNTTGG	2 cut(s)	1104, 1136
Alw21I	GWGCWC	2 cut(s)	1000, 1437
Alw26I	GTCTC	2 cut(s)	189, 410
AlwI	GGATC	2 cut(s)	667, 1813
AlwNI	CAGNNNCTG	1 cut(s)	1094
Aor51HI	AGCGCT	1 cut(s)	208
AoxI	GGCC	7 cut(s)	367, 574, 736, 1055, 1201, 1755, 1902
ApeKI	GCWGC	6 cut(s)	44, 266, 538, 1325, 1487, 2030
ApoI	RAATTY	4 cut(s)	139, 182, 795, 1537
ArsI	GACNNNNNNTTYG	2 cut(s)	275, 307
AsiGI	ACCGGT	1 cut(s)	1544
Asp700I	GAANNNNTTC	2 cut(s)	1383, 1949
Asp718I	GGTACC	1 cut(s)	5
AspA2I	CCTAGG	1 cut(s)	1130
AspLEI	GCGC	1 cut(s)	209
AspS9I	GGNCC	4 cut(s)	575, 1055, 1389, 1903
AsuHPI	GGTGA	4 cut(s)	226, 665, 740, 1534
AsuII	TTCGAA	1 cut(s)	1624
AvaII	GGWCC	1 cut(s)	1389
AvrII	CCTAGG	1 cut(s)	1130
BalI	TGGCCA	2 cut(s)	369, 1757
BanI	GGYRCC	1 cut(s)	5
BanII	GRGCYC	1 cut(s)	2004
BbsI	GAAGAC	2 cut(s)	641, 950
Bbv12I	GWGCWC	2 cut(s)	1000, 1437
BbvI	GCAGC	5 cut(s)	31, 253, 525, 1312, 1499
BccI	CCATC	8 cut(s)	650, 900, 1046, 1150, 1165, 1504, 1747, 1918
BcgI	CGANNNNNNTGC	2 cut(s)	1466, 1500
BciT130I	CCWGG	6 cut(s)	556, 902, 1032, 1265, 1797, 1823
BciVI	GTATCC	1 cut(s)	1227
BclI	TGATCA	1 cut(s)	862
BcoDI	GTCTC	2 cut(s)	189, 410
BfaI	CTAG	4 cut(s)	347, 789, 1101, 1131
BfmI	CTRYAG	1 cut(s)	1426
BfoI	RGCGCY	1 cut(s)	210
BfuI	GTATCC	1 cut(s)	1227
BglII	AGATCT	1 cut(s)	1674
BisI	GCNGC	8 cut(s)	45, 267, 539, 1259, 1326, 1488, 2028, 2031
BlnI	CCTAGG	1 cut(s)	1130
BlsI	GCNGC	8 cut(s)	46, 268, 540, 1260, 1327, 1489, 2029, 2032
BmcAI	AGTACT	1 cut(s)	919
Bme1390I	CCNGG	6 cut(s)	556, 902, 1032, 1265, 1797, 1823
Bme18I	GGWCC	1 cut(s)	1389
BmgT120I	GGNCC	4 cut(s)	575, 1055, 1389, 1903
BmiI	GGNNCC	5 cut(s)	7, 577, 1029, 1262, 1905
BmrFI	CCNGG	6 cut(s)	556, 902, 1032, 1265, 1797, 1823
BmrI	ACTGGG	1 cut(s)	1845
BmsI	GCATC	6 cut(s)	68, 418, 495, 1474, 1918, 1940
BmuI	ACTGGG	1 cut(s)	1845
BpiI	GAAGAC	2 cut(s)	641, 950
BplI	GAGNNNNNCTC	4 cut(s)	987, 1019, 1245, 1277
BpmI	CTGGAG	5 cut(s)	60, 516, 1247, 1350, 1844
Bpu14I	TTCGAA	1 cut(s)	1624
BpuEI	CTTGAG	1 cut(s)	145
BsaJI	CCNNGG	8 cut(s)	555, 699, 901, 1130, 1413, 1572, 1795, 1890
BsaWI	WCCGGW	1 cut(s)	1544
Bsc4I	CCNNNNNNNGG	1 cut(s)	650
Bse118I	RCCGGY	1 cut(s)	1544
Bse1I	ACTGG	5 cut(s)	43, 370, 1342, 1468, 1851
Bse3DI	GCAATG	1 cut(s)	54
BseBI	CCWGG	6 cut(s)	556, 902, 1032, 1265, 1797, 1823
BseDI	CCNNGG	8 cut(s)	555, 699, 901, 1130, 1413, 1572, 1795, 1890
BseLI	CCNNNNNNNGG	1 cut(s)	650
BseMI	GCAATG	1 cut(s)	54
BseMII	CTCAG	4 cut(s)	470, 1163, 1289, 1660
BseNI	ACTGG	5 cut(s)	43, 370, 1342, 1468, 1851
BseRI	GAGGAG	1 cut(s)	784
BseXI	GCAGC	5 cut(s)	31, 253, 525, 1312, 1499
BsgI	GTGCAG	1 cut(s)	1594
BshFI	GGCC	7 cut(s)	369, 576, 738, 1057, 1203, 1757, 1904
BshNI	GGYRCC	1 cut(s)	5
BshTI	ACCGGT	1 cut(s)	1544
BsiHKAI	GWGCWC	2 cut(s)	1000, 1437
BsiSI	CCGG	1 cut(s)	1545
BslFI	GGGAC	1 cut(s)	769
BslI	CCNNNNNNNGG	1 cut(s)	650
BsmAI	GTCTC	2 cut(s)	189, 410
BsmFI	GGGAC	1 cut(s)	769
BsmI	GAATGC	3 cut(s)	1023, 1137, 1632
BsnI	GGCC	7 cut(s)	369, 576, 738, 1057, 1203, 1757, 1904
Bsp119I	TTCGAA	1 cut(s)	1624
Bsp1286I	GDGCHC	3 cut(s)	1000, 1437, 2004
Bsp1407I	TGTACA	1 cut(s)	1830
Bsp143I	GATC	6 cut(s)	659, 862, 1502, 1642, 1674, 1818
Bsp19I	CCATGG	1 cut(s)	1572
BspACI	CCGC	3 cut(s)	9, 1258, 2027
BspANI	GGCC	7 cut(s)	369, 576, 738, 1057, 1203, 1757, 1904
BspCNI	CTCAG	4 cut(s)	469, 1162, 1288, 1659
BspHI	TCATGA	1 cut(s)	445
BspLI	GGNNCC	5 cut(s)	7, 577, 1029, 1262, 1905
BspMAI	CTGCAG	1 cut(s)	1430
BspPI	GGATC	2 cut(s)	667, 1813
BspQI	GCTCTTC	1 cut(s)	1575
BspT104I	TTCGAA	1 cut(s)	1624
BspT107I	GGYRCC	1 cut(s)	5
BsrDI	GCAATG	1 cut(s)	54
BsrFI	RCCGGY	1 cut(s)	1544
BsrGI	TGTACA	1 cut(s)	1830
BsrI	ACTGG	5 cut(s)	43, 370, 1342, 1468, 1851
BssAI	RCCGGY	1 cut(s)	1544
BssECI	CCNNGG	8 cut(s)	555, 699, 901, 1130, 1413, 1572, 1795, 1890
BssMI	GATC	6 cut(s)	659, 862, 1502, 1642, 1674, 1818
BssT1I	CCWWGG	4 cut(s)	1130, 1413, 1572, 1890
Bst2UI	CCWGG	6 cut(s)	556, 902, 1032, 1265, 1797, 1823
Bst4CI	ACNGT	3 cut(s)	134, 653, 807
Bst6I	CTCTTC	2 cut(s)	645, 1575
BstAUI	TGTACA	1 cut(s)	1830
BstBI	TTCGAA	1 cut(s)	1624
BstC8I	GCNNGC	3 cut(s)	49, 335, 355
BstDEI	CTNAG	6 cut(s)	456, 1149, 1275, 1616, 1646, 1935
BstDSI	CCRYGG	1 cut(s)	1572
BstH2I	RGCGCY	1 cut(s)	210
BstHHI	GCGC	1 cut(s)	209
BstKTI	GATC	6 cut(s)	662, 865, 1505, 1645, 1677, 1821
BstMAI	GTCTC	2 cut(s)	189, 410
BstMBI	GATC	6 cut(s)	659, 862, 1502, 1642, 1674, 1818
BstMWI	GCNNNNNNNGC	3 cut(s)	491, 1656, 2027
BstNI	CCWGG	6 cut(s)	556, 902, 1032, 1265, 1797, 1823
BstNSI	RCATGY	2 cut(s)	341, 357
BstSCI	CCNGG	6 cut(s)	554, 900, 1030, 1263, 1795, 1821
BstSFI	CTRYAG	1 cut(s)	1426
BstV1I	GCAGC	5 cut(s)	31, 253, 525, 1312, 1499
BstV2I	GAAGAC	2 cut(s)	641, 950
BstX2I	RGATCY	2 cut(s)	1674, 1818
BstXI	CCANNNNNNTGG	2 cut(s)	1399, 1573
BstYI	RGATCY	2 cut(s)	1674, 1818
BsuI	GTATCC	1 cut(s)	1227
BsuRI	GGCC	7 cut(s)	369, 576, 738, 1057, 1203, 1757, 1904
BtgI	CCRYGG	1 cut(s)	1572
BtsI	GCAGTG	1 cut(s)	998
BtsIMutI	CAGTG	2 cut(s)	363, 998
Cac8I	GCNNGC	3 cut(s)	49, 335, 355
CaiI	CAGNNNCTG	1 cut(s)	1094
CciI	TCATGA	1 cut(s)	445
CfoI	GCGC	1 cut(s)	209
Cfr10I	RCCGGY	1 cut(s)	1544
Cfr13I	GGNCC	4 cut(s)	575, 1055, 1389, 1903
Csp6I	GTAC	7 cut(s)	6, 918, 1404, 1480, 1684, 1831, 1840
CspAI	ACCGGT	1 cut(s)	1544
CviQI	GTAC	7 cut(s)	6, 918, 1404, 1480, 1684, 1831, 1840
DdeI	CTNAG	6 cut(s)	456, 1149, 1275, 1616, 1646, 1935
DpnI	GATC	6 cut(s)	661, 864, 1504, 1644, 1676, 1820
DpnII	GATC	6 cut(s)	659, 862, 1502, 1642, 1674, 1818
EaeI	YGGCCR	2 cut(s)	367, 1755
Eam1104I	CTCTTC	2 cut(s)	645, 1575
EarI	CTCTTC	2 cut(s)	645, 1575
Eco130I	CCWWGG	4 cut(s)	1130, 1413, 1572, 1890
Eco147I	AGGCCT	1 cut(s)	1203
Eco24I	GRGCYC	1 cut(s)	2004
Eco47I	GGWCC	1 cut(s)	1389
Eco47III	AGCGCT	1 cut(s)	208
Eco57I	CTGAAG	1 cut(s)	1184
EcoRII	CCWGG	6 cut(s)	554, 900, 1030, 1263, 1795, 1821
EcoT14I	CCWWGG	4 cut(s)	1130, 1413, 1572, 1890
EcoT22I	ATGCAT	1 cut(s)	1933
EcoT38I	GRGCYC	1 cut(s)	2004
ErhI	CCWWGG	4 cut(s)	1130, 1413, 1572, 1890
FalI	AAGNNNNNCTT	2 cut(s)	1751, 1783
FaqI	GGGAC	1 cut(s)	769
FauNDI	CATATG	3 cut(s)	520, 532, 1778
FbaI	TGATCA	1 cut(s)	862
FblI	GTMKAC	1 cut(s)	744
Fnu4HI	GCNGC	8 cut(s)	45, 267, 539, 1259, 1326, 1488, 2028, 2031
FriOI	GRGCYC	1 cut(s)	2004
Fsp4HI	GCNGC	8 cut(s)	45, 267, 539, 1259, 1326, 1488, 2028, 2031
FspBI	CTAG	4 cut(s)	347, 789, 1101, 1131
GlaI	GCGC	1 cut(s)	208
GluI	GCNGC	8 cut(s)	45, 267, 539, 1259, 1326, 1488, 2028, 2031
GsuI	CTGGAG	5 cut(s)	60, 516, 1247, 1350, 1844
HaeII	RGCGCY	1 cut(s)	210
HaeIII	GGCC	7 cut(s)	369, 576, 738, 1057, 1203, 1757, 1904
HapII	CCGG	1 cut(s)	1545
HhaI	GCGC	1 cut(s)	209
Hin6I	GCGC	1 cut(s)	207
HinP1I	GCGC	1 cut(s)	207
HincII	GTYRAC	2 cut(s)	220, 283
HindII	GTYRAC	2 cut(s)	220, 283
HindIII	AAGCTT	2 cut(s)	122, 890
HpaII	CCGG	1 cut(s)	1545
HphI	GGTGA	4 cut(s)	226, 665, 740, 1534
Hpy166II	GTNNAC	4 cut(s)	220, 283, 312, 745
Hpy188I	TCNGA	9 cut(s)	645, 867, 913, 1075, 1152, 1164, 1296, 1389, 1642
Hpy8I	GTNNAC	4 cut(s)	220, 283, 312, 745
HpyAV	CCTTC	5 cut(s)	161, 239, 926, 1214, 1765
HpyCH4III	ACNGT	3 cut(s)	134, 653, 807
HpyCH4IV	ACGT	3 cut(s)	526, 1270, 1992
HpyCH4V	TGCA	8 cut(s)	59, 72, 259, 357, 1428, 1487, 1611, 1931
HpyF10VI	GCNNNNNNNGC	3 cut(s)	491, 1656, 2027
HpyF3I	CTNAG	6 cut(s)	456, 1149, 1275, 1616, 1646, 1935
HpySE526I	ACGT	3 cut(s)	526, 1270, 1992
HspAI	GCGC	1 cut(s)	207
KpnI	GGTACC	1 cut(s)	9
Ksp22I	TGATCA	1 cut(s)	862
Kzo9I	GATC	6 cut(s)	659, 862, 1502, 1642, 1674, 1818
LguI	GCTCTTC	1 cut(s)	1575
LmnI	GCTCC	6 cut(s)	41, 497, 995, 1008, 1132, 1266
Lsp1109I	GCAGC	5 cut(s)	31, 253, 525, 1312, 1499
LweI	GCATC	6 cut(s)	68, 418, 495, 1474, 1918, 1940
MaeI	CTAG	4 cut(s)	347, 789, 1101, 1131
MaeII	ACGT	3 cut(s)	526, 1270, 1992
MaeIII	GTNAC	5 cut(s)	296, 361, 1333, 1808, 1913
MalI	GATC	6 cut(s)	661, 864, 1504, 1644, 1676, 1820
MboI	GATC	6 cut(s)	659, 862, 1502, 1642, 1674, 1818
MflI	RGATCY	2 cut(s)	1674, 1818
MhlI	GDGCHC	3 cut(s)	1000, 1437, 2004
MlsI	TGGCCA	2 cut(s)	369, 1757
MluNI	TGGCCA	2 cut(s)	369, 1757
MlyI	GAGTC	4 cut(s)	248, 631, 1816, 1985
Mox20I	TGGCCA	2 cut(s)	369, 1757
Mph1103I	ATGCAT	1 cut(s)	1933
MroXI	GAANNNNTTC	2 cut(s)	1383, 1949
MscI	TGGCCA	2 cut(s)	369, 1757
MseI	TTAA	6 cut(s)	858, 894, 1302, 1866, 1986, 2038
MslI	CAYNNNNRTG	2 cut(s)	654, 726
Msp20I	TGGCCA	2 cut(s)	369, 1757
MspI	CCGG	1 cut(s)	1545
MspR9I	CCNGG	6 cut(s)	556, 902, 1032, 1265, 1797, 1823
Mva1269I	GAATGC	3 cut(s)	1023, 1137, 1632
MvaI	CCWGG	6 cut(s)	556, 902, 1032, 1265, 1797, 1823
MwoI	GCNNNNNNNGC	3 cut(s)	491, 1656, 2027
NcoI	CCATGG	1 cut(s)	1572
NdeI	CATATG	3 cut(s)	520, 532, 1778
NdeII	GATC	6 cut(s)	659, 862, 1502, 1642, 1674, 1818
NlaIV	GGNNCC	5 cut(s)	7, 577, 1029, 1262, 1905
NmeAIII	GCCGAG	1 cut(s)	724
NmuCI	GTSAC	3 cut(s)	361, 1333, 1808
NsiI	ATGCAT	1 cut(s)	1933
NspI	RCATGY	2 cut(s)	341, 357
NspV	TTCGAA	1 cut(s)	1624
PaeI	GCATGC	1 cut(s)	357
PagI	TCATGA	1 cut(s)	445
PceI	AGGCCT	1 cut(s)	1203
PciSI	GCTCTTC	1 cut(s)	1575
PctI	GAATGC	3 cut(s)	1023, 1137, 1632
PdmI	GAANNNNTTC	2 cut(s)	1383, 1949
PfeI	GAWTC	6 cut(s)	601, 646, 967, 1076, 1097, 1364
PflFI	GACNNNGTC	2 cut(s)	215, 1604
PfoI	TCCNGGA	3 cut(s)	1030, 1263, 1821
PinAI	ACCGGT	1 cut(s)	1544
PkrI	GCNGC	8 cut(s)	46, 268, 540, 1260, 1327, 1489, 2029, 2032
PleI	GAGTC	4 cut(s)	247, 631, 1815, 1984
PpsI	GAGTC	4 cut(s)	247, 631, 1815, 1984
Psp1406I	AACGTT	1 cut(s)	1270
Psp6I	CCWGG	6 cut(s)	554, 900, 1030, 1263, 1795, 1821
PspGI	CCWGG	6 cut(s)	554, 900, 1030, 1263, 1795, 1821
PspN4I	GGNNCC	5 cut(s)	7, 577, 1029, 1262, 1905
PspPI	GGNCC	4 cut(s)	575, 1055, 1389, 1903
PstI	CTGCAG	1 cut(s)	1430
PstNI	CAGNNNCTG	1 cut(s)	1094
PsuI	RGATCY	2 cut(s)	1674, 1818
PsyI	GACNNNGTC	2 cut(s)	215, 1604
RsaI	GTAC	7 cut(s)	7, 919, 1405, 1481, 1685, 1832, 1841
RsaNI	GTAC	7 cut(s)	6, 918, 1404, 1480, 1684, 1831, 1840
RseI	CAYNNNNRTG	2 cut(s)	654, 726
SapI	GCTCTTC	1 cut(s)	1575
SaqAI	TTAA	6 cut(s)	858, 894, 1302, 1866, 1986, 2038
SatI	GCNGC	8 cut(s)	45, 267, 539, 1259, 1326, 1488, 2028, 2031
Sau3AI	GATC	6 cut(s)	659, 862, 1502, 1642, 1674, 1818
Sau96I	GGNCC	4 cut(s)	575, 1055, 1389, 1903
ScaI	AGTACT	1 cut(s)	919
SchI	GAGTC	4 cut(s)	248, 631, 1816, 1985
ScrFI	CCNGG	6 cut(s)	556, 902, 1032, 1265, 1797, 1823
SduI	GDGCHC	3 cut(s)	1000, 1437, 2004
SfaNI	GCATC	6 cut(s)	68, 418, 495, 1474, 1918, 1940
SfcI	CTRYAG	1 cut(s)	1426
SfuI	TTCGAA	1 cut(s)	1624
SinI	GGWCC	1 cut(s)	1389
SmiMI	CAYNNNNRTG	2 cut(s)	654, 726
SmlI	CTYRAG	1 cut(s)	160
SmoI	CTYRAG	1 cut(s)	160
SphI	GCATGC	1 cut(s)	357
SseBI	AGGCCT	1 cut(s)	1203
SsiI	CCGC	3 cut(s)	9, 1258, 2027
SspMI	CTAG	4 cut(s)	347, 789, 1101, 1131
StuI	AGGCCT	1 cut(s)	1203
StyD4I	CCNGG	6 cut(s)	554, 900, 1030, 1263, 1795, 1821
StyI	CCWWGG	4 cut(s)	1130, 1413, 1572, 1890
TaaI	ACNGT	3 cut(s)	134, 653, 807
TaiI	ACGT	3 cut(s)	529, 1273, 1995
TaqI	TCGA	2 cut(s)	1476, 1624
TatI	WGTACW	4 cut(s)	917, 1683, 1830, 1839
TauI	GCSGC	2 cut(s)	1261, 2030
TfiI	GAWTC	6 cut(s)	601, 646, 967, 1076, 1097, 1364
Tru1I	TTAA	6 cut(s)	858, 894, 1302, 1866, 1986, 2038
Tru9I	TTAA	6 cut(s)	858, 894, 1302, 1866, 1986, 2038
TscAI	CASTG	2 cut(s)	370, 1005
TseFI	GTSAC	3 cut(s)	361, 1333, 1808
TseI	GCWGC	6 cut(s)	44, 266, 538, 1325, 1487, 2030
Tsp45I	GTSAC	3 cut(s)	361, 1333, 1808
TspRI	CASTG	2 cut(s)	370, 1005
Tth111I	GACNNNGTC	2 cut(s)	215, 1604
VpaK11BI	GGWCC	1 cut(s)	1389
XapI	RAATTY	4 cut(s)	139, 182, 795, 1537
XbaI	TCTAGA	1 cut(s)	346
XceI	RCATGY	2 cut(s)	341, 357
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1573, 1897
XmaJI	CCTAGG	1 cut(s)	1130
XmiI	GTMKAC	1 cut(s)	744
XmnI	GAANNNNTTC	2 cut(s)	1383, 1949
XspI	CTAG	4 cut(s)	347, 789, 1101, 1131
ZrmI	AGTACT	1 cut(s)	919
Zsp2I	ATGCAT	1 cut(s)	1933

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.