Rorug06G0338000

rhamnose biosynthetic enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0002188
Physical Location & Seq
Reverse (-)
48997836 .. 48999827
1992 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0338000.1

Sequence Viewer

Length: 1326 bp
ATGGCTATCGTGAAAGTACGTGGAACCCTAAAAGACGGTTTATTTACTCCAAAATCGAAGAGGCCAAGAGTGGATGATTTTGGAAATTTTCCCAATAGGCGTGGATCATATAGAGTTATGGTGCAAAGTAGTGAAAGTGAAGATCACAATTCTCTACTTGATCGTGATTTGGTTAAATCATCAAAAAATCTCCAACAAGGTAAGAGAAATGATGGGATTTCGCATTTTTCTATTACGGCTGCACCACGAGAAATTTTGCCCTCACCACAGAGTGTATTGAAAAGTAACTCTGTAAAGAGCAAGGAGGATCAGAACACTAACCTATCAATCCGCCAAGGATTGAGGAAGCGACAGCCTTTGTCTGATGAATACCGACCTGAAAGGATGGCTGTATGTGGAAATTTTGATTTTAGTCACCAGCATGTGCATAATTTGGAAGATAAAGATAATACCGAGGTGAAGAAGGATCTTGATGTGATCAAAGATACTTTGGCCAACTCGAAGAAACAAAATTGGAGCAACGAATATCAGCACAAAGCCAACACAGTGTCCAAAAATGTTGTTACAACTTTTAATTCAAAAAATAGGCATTTCGTCTTGGGGAGAAAGAGACAACCACTGTGGGACCACCAGCAACCTTACCAGACGAGTATGCATGCGGAGTTTACAAGTCAAAAGTCGGTGGAAGTGGAACATAAAGATCGTAACAAGGTCAAGGGGGCTTTGGAAGCCTATAGAGAGATATTGACTAAATCCGAGGCACAAAACTGTAATTGGAGTTACATTAAAGCAGCAATGGATCTCAAAAAACAAGGAAAATGGGTTAACAAGATGAAGCAAGTAGGATCGATTGCAGGAGTAAAAGTTGGTGATAGATTTCGACATAGAGCTCAACTAACCATTGTTGGTCTTCATCGACAATTTACATGTGGTATTGATTATATGTGGAGGAATGACGGAAAAATTTTGGCCACAAGTATTGTTGATTCTGGTCGCTATGTTAACAATTTGCAATCTTCTGATATACTGATCTACTCTGGTCAAGGTGGAAAGTTTCTGTTTCATAAAACTAAACCGACAGATCAAACTAAACCGACAGATCAAATAATGCAAGGTGGAAACCTTGCATTAAGGAATAGCAAAGAAGAAGGAACTCCTATAAGGGTAATTCGAAGGTTGAAACATTCTAATGGTTCGTGTTATGTTTACTATGGATTATACACCGTAGAAAGAGTTCTGCAAGAAAGACAACTTGGTATTATGATCTATAAATTTATGTTGAGAAGGATGTCGGGACAACCAGAGCTTTCATTTGACAGTAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00520 map00520
Pfam Domains
Protein Families
Epimerase GDP_Man_Dehyd

Protein Analysis

441

Amino Acids

50.65

Weight (kDa)

9.86

Isoelectric Point (pI)

43.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAD_SRA PF02182 281 - 433 6.5e-41 SAD/SRA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 331, 659
AclWI GGATC 5 cut(s) 112, 315, 474, 807, 853
AcoI YGGCCR 2 cut(s) 492, 969
AcsI RAATTY 5 cut(s) 85, 252, 400, 963, 1271
AdeI CACNNNGTG 1 cut(s) 272
AfaI GTAC 1 cut(s) 18
AflIII ACRYGT 1 cut(s) 926
AgsI TTSAA 3 cut(s) 280, 579, 1180
AjuI GAANNNNNNNTTGG 2 cut(s) 1236, 1268
AluBI AGCT 2 cut(s) 890, 1306
AluI AGCT 2 cut(s) 890, 1306
Alw21I GWGCWC 1 cut(s) 892
Alw26I GTCTC 1 cut(s) 604
AlwI GGATC 5 cut(s) 112, 315, 474, 807, 853
AoxI GGCC 3 cut(s) 62, 492, 969
ApeKI GCWGC 2 cut(s) 239, 791
ApoI RAATTY 5 cut(s) 85, 252, 400, 963, 1271
Asp700I GAANNNNTTC 1 cut(s) 1233
AspS9I GGNCC 1 cut(s) 625
AsuHPI GGTGA 4 cut(s) 255, 407, 469, 881
AsuII TTCGAA 1 cut(s) 1171
AvaII GGWCC 1 cut(s) 625
BalI TGGCCA 2 cut(s) 494, 971
BanII GRGCYC 1 cut(s) 892
BauI CACGAG 1 cut(s) 246
BbsI GAAGAC 1 cut(s) 902
Bbv12I GWGCWC 1 cut(s) 892
BbvI GCAGC 2 cut(s) 226, 803
BccI CCATC 2 cut(s) 206, 379
BceAI ACGGC 1 cut(s) 252
BclI TGATCA 1 cut(s) 477
BcoDI GTCTC 1 cut(s) 604
BfmI CTRYAG 1 cut(s) 733
BisI GCNGC 2 cut(s) 240, 792
BlsI GCNGC 2 cut(s) 241, 793
Bme18I GGWCC 1 cut(s) 625
BmgT120I GGNCC 1 cut(s) 625
BmiI GGNNCC 2 cut(s) 25, 626
BpiI GAAGAC 1 cut(s) 902
Bpu14I TTCGAA 1 cut(s) 1171
Bsa29I ATCGAT 1 cut(s) 848
BsaAI YACGTR 1 cut(s) 20
BsaJI CCNNGG 3 cut(s) 334, 453, 756
BsaXI ACNNNNNCTCC 2 cut(s) 849, 879
Bse3DI GCAATG 1 cut(s) 801
BseCI ATCGAT 1 cut(s) 848
BseDI CCNNGG 3 cut(s) 334, 453, 756
BseGI GGATG 3 cut(s) 79, 390, 1293
BseMI GCAATG 1 cut(s) 801
BseXI GCAGC 2 cut(s) 226, 803
BsgI GTGCAG 1 cut(s) 225
BshFI GGCC 3 cut(s) 64, 494, 971
BshVI ATCGAT 1 cut(s) 848
BsiHKAI GWGCWC 1 cut(s) 892
BslFI GGGAC 2 cut(s) 638, 1308
BsmAI GTCTC 1 cut(s) 604
BsmFI GGGAC 2 cut(s) 638, 1308
BsnI GGCC 3 cut(s) 64, 494, 971
Bsp119I TTCGAA 1 cut(s) 1171
Bsp1286I GDGCHC 1 cut(s) 892
BspACI CCGC 2 cut(s) 331, 659
BspANI GGCC 3 cut(s) 64, 494, 971
BspDI ATCGAT 1 cut(s) 848
BspLI GGNNCC 2 cut(s) 25, 626
BspPI GGATC 5 cut(s) 112, 315, 474, 807, 853
BspT104I TTCGAA 1 cut(s) 1171
BsrDI GCAATG 1 cut(s) 801
BssECI CCNNGG 3 cut(s) 334, 453, 756
BssSI CACGAG 1 cut(s) 246
BssT1I CCWWGG 1 cut(s) 334
Bst2BI CACGAG 1 cut(s) 246
Bst4CI ACNGT 6 cut(s) 38, 547, 621, 770, 1225, 1319
Bst6I CTCTTC 1 cut(s) 53
BstBAI YACGTR 1 cut(s) 20
BstBI TTCGAA 1 cut(s) 1171
BstC8I GCNNGC 1 cut(s) 657
BstF5I GGATG 3 cut(s) 79, 390, 1293
BstMAI GTCTC 1 cut(s) 604
BstMWI GCNNNNNNNGC 1 cut(s) 728
BstNSI RCATGY 3 cut(s) 425, 659, 930
BstSFI CTRYAG 1 cut(s) 733
BstV1I GCAGC 2 cut(s) 226, 803
BstV2I GAAGAC 1 cut(s) 902
BstX2I RGATCY 2 cut(s) 466, 799
BstYI RGATCY 2 cut(s) 466, 799
Bsu15I ATCGAT 1 cut(s) 848
BsuRI GGCC 3 cut(s) 64, 494, 971
BsuTUI ATCGAT 1 cut(s) 848
BtsCI GGATG 3 cut(s) 79, 390, 1293
BtsIMutI CAGTG 2 cut(s) 552, 617
Cac8I GCNNGC 1 cut(s) 657
Cfr13I GGNCC 1 cut(s) 625
ClaI ATCGAT 1 cut(s) 848
Csp6I GTAC 1 cut(s) 17
CspCI CAANNNNNGTGG 6 cut(s) 82, 117, 602, 637, 961, 996
CviAII CATG 3 cut(s) 422, 656, 927
CviQI GTAC 1 cut(s) 17
DraIII CACNNNGTG 1 cut(s) 272
EaeI YGGCCR 2 cut(s) 492, 969
Eam1104I CTCTTC 1 cut(s) 53
EarI CTCTTC 1 cut(s) 53
EciI GGCGGA 1 cut(s) 320
Ecl136II GAGCTC 1 cut(s) 890
Eco130I CCWWGG 1 cut(s) 334
Eco24I GRGCYC 1 cut(s) 892
Eco47I GGWCC 1 cut(s) 625
Eco53kI GAGCTC 1 cut(s) 890
EcoICRI GAGCTC 1 cut(s) 890
EcoT14I CCWWGG 1 cut(s) 334
EcoT22I ATGCAT 1 cut(s) 657
EcoT38I GRGCYC 1 cut(s) 892
ErhI CCWWGG 1 cut(s) 334
FaeI CATG 3 cut(s) 425, 659, 930
FaqI GGGAC 2 cut(s) 638, 1308
FatI CATG 3 cut(s) 421, 655, 926
FbaI TGATCA 1 cut(s) 477
Fnu4HI GCNGC 2 cut(s) 240, 792
FokI GGATG 3 cut(s) 86, 397, 1300
FriOI GRGCYC 1 cut(s) 892
Fsp4HI GCNGC 2 cut(s) 240, 792
GluI GCNGC 2 cut(s) 240, 792
HaeIII GGCC 3 cut(s) 64, 494, 971
Hin1II CATG 3 cut(s) 425, 659, 930
HincII GTYRAC 2 cut(s) 826, 1003
HindII GTYRAC 2 cut(s) 826, 1003
HinfI GANTC 1 cut(s) 986
HpaI GTTAAC 2 cut(s) 826, 1003
HphI GGTGA 4 cut(s) 255, 407, 469, 881
Hpy166II GTNNAC 4 cut(s) 666, 826, 1003, 1207
Hpy188I TCNGA 4 cut(s) 312, 364, 757, 1021
Hpy188III TCNNGA 4 cut(s) 10, 164, 470, 1293
Hpy8I GTNNAC 4 cut(s) 666, 826, 1003, 1207
HpyAV CCTTC 4 cut(s) 457, 1142, 1167, 1278
HpyCH4III ACNGT 6 cut(s) 38, 547, 621, 770, 1225, 1319
HpyCH4IV ACGT 1 cut(s) 19
HpyCH4V TGCA 9 cut(s) 124, 242, 427, 655, 854, 1012, 1111, 1127, 1240
HpyF10VI GCNNNNNNNGC 1 cut(s) 728
HpySE526I ACGT 1 cut(s) 19
Hsp92II CATG 3 cut(s) 425, 659, 930
Ksp22I TGATCA 1 cut(s) 477
KspAI GTTAAC 2 cut(s) 826, 1003
LmnI GCTCC 1 cut(s) 516
LpnPI CCDG 8 cut(s) 390, 431, 644, 656, 840, 975, 1023, 1314
Lsp1109I GCAGC 2 cut(s) 226, 803
MaeII ACGT 1 cut(s) 19
MaeIII GTNAC 5 cut(s) 284, 413, 562, 704, 779
MboII GAAGA 8 cut(s) 70, 152, 449, 472, 514, 902, 1008, 1157
MflI RGATCY 2 cut(s) 466, 799
MhlI GDGCHC 1 cut(s) 892
MlsI TGGCCA 2 cut(s) 494, 971
MluNI TGGCCA 2 cut(s) 494, 971
MmeI TCCRAC 1 cut(s) 217
MnlI CCTC 7 cut(s) 54, 271, 298, 336, 448, 751, 942
Mox20I TGGCCA 2 cut(s) 494, 971
Mph1103I ATGCAT 1 cut(s) 657
MroXI GAANNNNTTC 1 cut(s) 1233
MscI TGGCCA 2 cut(s) 494, 971
MseI TTAA 6 cut(s) 174, 573, 786, 825, 1002, 1130
MslI CAYNNNNRTG 2 cut(s) 420, 1188
Msp20I TGGCCA 2 cut(s) 494, 971
MwoI GCNNNNNNNGC 1 cut(s) 728
NlaIII CATG 3 cut(s) 425, 659, 930
NlaIV GGNNCC 2 cut(s) 25, 626
NmuCI GTSAC 1 cut(s) 413
NsiI ATGCAT 1 cut(s) 657
NspI RCATGY 3 cut(s) 425, 659, 930
NspV TTCGAA 1 cut(s) 1171
PaeI GCATGC 1 cut(s) 659
PciI ACATGT 1 cut(s) 926
PdmI GAANNNNTTC 1 cut(s) 1233
PfeI GAWTC 1 cut(s) 986
PkrI GCNGC 2 cut(s) 241, 793
Ppu21I YACGTR 1 cut(s) 20
PscI ACATGT 1 cut(s) 926
Psp124BI GAGCTC 1 cut(s) 892
PspN4I GGNNCC 2 cut(s) 25, 626
PspPI GGNCC 1 cut(s) 625
PsrI GAACNNNNNNTAC 2 cut(s) 1218, 1250
PsuI RGATCY 2 cut(s) 466, 799
RsaI GTAC 1 cut(s) 18
RsaNI GTAC 1 cut(s) 17
RseI CAYNNNNRTG 2 cut(s) 420, 1188
SacI GAGCTC 1 cut(s) 892
SaqAI TTAA 6 cut(s) 174, 573, 786, 825, 1002, 1130
SatI GCNGC 2 cut(s) 240, 792
Sau96I GGNCC 1 cut(s) 625
SduI GDGCHC 1 cut(s) 892
SfcI CTRYAG 1 cut(s) 733
SfuI TTCGAA 1 cut(s) 1171
SinI GGWCC 1 cut(s) 625
SmiMI CAYNNNNRTG 2 cut(s) 420, 1188
SphI GCATGC 1 cut(s) 659
SsiI CCGC 2 cut(s) 331, 659
SstI GAGCTC 1 cut(s) 892
StyI CCWWGG 1 cut(s) 334
TaaI ACNGT 6 cut(s) 38, 547, 621, 770, 1225, 1319
TaiI ACGT 1 cut(s) 22
TaqI TCGA 6 cut(s) 56, 500, 848, 880, 916, 1171
TfiI GAWTC 1 cut(s) 986
Tru1I TTAA 6 cut(s) 174, 573, 786, 825, 1002, 1130
Tru9I TTAA 6 cut(s) 174, 573, 786, 825, 1002, 1130
TscAI CASTG 2 cut(s) 552, 624
TseFI GTSAC 1 cut(s) 413
TseI GCWGC 2 cut(s) 239, 791
Tsp45I GTSAC 1 cut(s) 413
TspDTI ATGAA 5 cut(s) 381, 848, 902, 1052, 1299
TspGWI ACGGA 1 cut(s) 972
TspRI CASTG 2 cut(s) 552, 624
VpaK11BI GGWCC 1 cut(s) 625
XapI RAATTY 5 cut(s) 85, 252, 400, 963, 1271
XceI RCATGY 3 cut(s) 425, 659, 930
XmnI GAANNNNTTC 1 cut(s) 1233
Zsp2I ATGCAT 1 cut(s) 657
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.