Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0002188

Physical Location & Seq

Genomic Coordinates Reverse (-)

66030690 .. 66033673

Gene Structure 2984 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000020533

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2073 bp

ATGGAGTCAGGACAAGCAATAGCTCTTTCAGATATGGGCACCGCCTATAAACCGAAGAACATCCTCATTACTGGAGCTGCTGGCTTCATCGCATCCCATGTTTGCAATCGGCTTATCAGGAACTACCCTGAATACAAGATTGTCGTCCTTGACAAGCTTGATTACTGTTCAAATTTGAAGAACCTTCTTCCCTCAAGGTCATCCCCAAACTTCAAATTTATCAAGGGAGACATTGGAAGCGCTGACCTTGTCAACTTCATCCTTCTCACCGAGTCCATTGATACAATAATGCACTTTGCTGCCCAGACCCATGTTGACAACTCATTTGGTAACAGCTTTGAGTTCACTAAAAACAACATATATGGCACGCATGTTCTTCTAGAAGCATGCAAAGTCACGGGCCAGATCAAAAGGTTCATCCATGTTAGCACAGATGAAGTTTATGGGGAGACAGATGAGGATGCTGTGGTGGGAAATCATGAGGCTTCTCAGCTTCTTCCCACAAACCCCTACTCTGCGACCAAAGCTGGAGCAGAGATGCTTGTTATGGCATATGGACGTTCATATGGGCTGCCTGTGATAACTACCAGGGGAAACAATGTTTATGGCCCCAATCAGTTCCCTGAAAAGATGATTCCAAAGTTCATTCTCTTGGCTATGAAAGGGAAGACTCTTCCGATTCACGGTGATGGATCAAATGTCAGGAGTTATCTCTATTGTGAGGATGTAGCCGAGGCATTTGAGGTCATTCTCCATAAGGGTGAGGTAGGCCATGTCTACAACATTGGGACAAAGAAAGAGAGGAGGGTAGTTGATGTCGCTAGGGAAATTTGCCAACTGTTCTCGTTGGACCCAGATACATATATAAACTTTGTTGAGAATAGACCTTTTAATGATCAGAGGTATTTTCTGGATGACCAAAAGCTTAAAAACTTGGGATGGTCTGAAAGTACTTCATGGGAGGAAGGTTTGAGGAAGACAATGGAATGGTACGTCAAGAATCCTCATTGGTGGGGAGATGTTTCTGGAGCACTGCTCCCTCATCCAAGAATGCTCATGGTTCCTGGAATTGAAAGAAACTTTGATGGGCCTGATACAAGCAATTCCGATTCCCCTGTGCCAGCAACTGATTCTAGTCAAAGCCAAGTGGTTGTTCCAGCTCCTAGGGGCATTCCATCTACTCAGAAGCCATCTCTGAAGTTCCTGATTTATGGGAGAACAGGGTGGATTGGAGGCCTTCTTGGGAAGATTTGTGAGAAACAAGGGATACCATTTGAGTATGGTCGAGGGCGGCTCCAGGAACGTTCTCAGCTCTTGGCTGATATTCAGAGGATTAAGCCAACCCATGTTTTCAATGCTGCTGGAGTGACTGGCAGACCCAATGTGGATTGGTGTGAATCTCATAAACCAGAAACAATTCGGACCAATGTTGTGGGTACATTGACATTGGCTGATGTCTGCAGAGAGCACAACCTCTTAATGATGAATTATGCTACTGGTTGTATCTTTGAGTACGATGCAGCTCATCCATCAAGATCAGGAATTGGGTTCAAGGAGGAAGATATACCAAATTTCACTGGTTCATTTTACTCCAAAACCAAAGCCATGGTGGAAGAGCTTTTGAAAGAATATGACAATGTCTGCACTCTTAGAGTTCGAATGCCTATATCATCTGATCTCAGCAATCCACGCAACTTCATCACAAAGATCTCCAAGTACAACAAAGTGGTTGATATTCCAAACAGCATGACTATCTTGGATGAACTTCTACCGATTTCAGTTGAGATGGCCAAGAGGAACTTGAAGGGCATTTGGAACTTCACAAACCCTGGTGTTGTGAGTCACAATGAGATCCTGGAGATGTACAAGAAGTACATAGACCCCAGTTTCAAGTGGGTTAATTTTACATTGGAAGAACAAGCCAAGGTTATTGTGGCCCCAAGAAGTAACAATGAGATGGATGCATCTAAGTTGAAGAAAGAGTTCCCTGAAGTATTGCCAATCAAGGAGTCGCTGATTAAATACGTCTTTGAGCCCAACAAGAAAACATTTGCCGGTGGAGCAGCAAATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005911 GO:0006139 GO:0006725 GO:0006793 GO:0006807 GO:0006810 GO:0008150 GO:0008152 GO:0009058 GO:0009225 GO:0009226 GO:0009506 GO:0009812 GO:0009813 GO:0009914 GO:0009966 GO:0009987 GO:0010253 GO:0010280 GO:0010315 GO:0010646 GO:0010817 GO:0010928 GO:0016829 GO:0016835 GO:0016836 GO:0018130 GO:0019438 GO:0023051 GO:0030054 GO:0030154 GO:0032502 GO:0033478 GO:0034641 GO:0034654 GO:0042127 GO:0042440 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046148 GO:0046483 GO:0048583 GO:0048869 GO:0050377 GO:0050789 GO:0050794 GO:0051179 GO:0051234 GO:0051552 GO:0051553 GO:0051554 GO:0051555 GO:0055044 GO:0055086 GO:0060918 GO:0065007 GO:0065008 GO:0071704 GO:1901135 GO:1901137 GO:1901360 GO:1901362 GO:1901576 GO:1901615 GO:1901617

KEGG Pathways

Metabolic & Signaling

ko00520 map00520

Pfam Domains

Protein Families

Epimerase GDP_Man_Dehyd

Protein Analysis

691

Amino Acids

77.51

Weight (kDa)

6.78

Isoelectric Point (pI)

33.58

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RmlD_sub_bind	PF04321	20 - 329	8.6e-14	RmlD substrate binding domain
Epimerase	PF01370	21 - 263	2.9e-56	NAD dependent epimerase/dehydratase family
Polysacc_synt_2	PF02719	21 - 291	7.9e-20	Polysaccharide biosynthesis protein
GDP_Man_Dehyd	PF16363	22 - 327	2.3e-65	GDP-mannose 4,6 dehydratase
3Beta_HSD	PF01073	22 - 247	4.5e-24	3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_4	PF07993	23 - 205	5.6e-19	Male sterility protein
RmlD_sub_bind	PF04321	400 - 569	4.2e-11	RmlD substrate binding domain
Epimerase	PF01370	429 - 564	3.6e-10	NAD dependent epimerase/dehydratase family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

Species Filter

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Orthologous Genes (Group: OG0002188)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G53500 AT3G14790 AT3G14790
fragaria_vesca	FvH4_2g36650 FvH4_2g36650 FvH4_6g38080 FvH4_6g38080 FvH4_6g38080 FvH4_6g38080 FvH4_6g38080
malus_domestica	MD09G1154300.v1.1 MD09G1154700.v1.1 MD15G1106300.v1.1 MD17G1140800.v1.1
prunus_persica	Prupe.1G458500_v2.0.a1 Prupe.3G170800_v2.0.a1
pyrus_communis	pycom09g07360 pycom15g09690 pycom17g13400
rosa_chinensis	RchiOBHm_Chr2g0151131 RchiOBHm_Chr6g0305141
rosa_laevigata	RLG00000010914 RLG00000020533
rosa_multiflora	Rmu_sc0000061.1_g000019 Rmu_sc0032179.1_g000002
rosa_roxburghii	Rroxscaffold_2G00096710 Rroxscaffold_7G00163120
rosa_rugosa	Rorug02G0425000 Rorug02G0425100 Rorug06G0338000 Rorug06G0338100 Rorug06G0338200 Rorug06G0338300
rosa_samantha	Rh2AG485600 Rh2BG497500 Rh2CG471400 Rh2DG508500 Rh6AG449700 Rh6BG451700 Rh6CG463100 Rh6DG450100
rosa_wichuraiana	Rw2G039770 Rw6G039160

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	38
AccI	GTMKAC	1 cut(s)	777
AciI	CCGC	2 cut(s)	42, 1291
AclI	AACGTT	1 cut(s)	1303
AclWI	GGATC	2 cut(s)	700, 1846
AcoI	YGGCCR	1 cut(s)	1788
AcsI	RAATTY	4 cut(s)	172, 215, 828, 1570
AcuI	CTGAAG	2 cut(s)	1217, 2010
AfaI	GTAC	7 cut(s)	952, 992, 1438, 1514, 1718, 1865, 1874
AfeI	AGCGCT	1 cut(s)	241
AfiI	CCNNNNNNNGG	1 cut(s)	683
AjnI	CCWGG	5 cut(s)	587, 1063, 1296, 1828, 1854
AjuI	GAANNNNNNNTTGG	2 cut(s)	1137, 1169
Alw21I	GWGCWC	2 cut(s)	1033, 1470
Alw26I	GTCTC	2 cut(s)	222, 443
AlwI	GGATC	2 cut(s)	700, 1846
AlwNI	CAGNNNCTG	1 cut(s)	1127
Aor51HI	AGCGCT	1 cut(s)	241
AoxI	GGCC	7 cut(s)	400, 607, 769, 1088, 1234, 1788, 1935
ApeKI	GCWGC	6 cut(s)	77, 299, 571, 1358, 1520, 2063
ApoI	RAATTY	4 cut(s)	172, 215, 828, 1570
ArsI	GACNNNNNNTTYG	2 cut(s)	308, 340
Asp700I	GAANNNNTTC	2 cut(s)	1416, 1982
AspA2I	CCTAGG	1 cut(s)	1163
AspLEI	GCGC	1 cut(s)	242
AspS9I	GGNCC	6 cut(s)	400, 608, 850, 1088, 1422, 1936
AsuHPI	GGTGA	3 cut(s)	259, 698, 773
AsuII	TTCGAA	1 cut(s)	1657
AvaII	GGWCC	2 cut(s)	850, 1422
AvrII	CCTAGG	1 cut(s)	1163
BaeGI	GKGCMC	1 cut(s)	41
BalI	TGGCCA	1 cut(s)	1790
BanI	GGYRCC	1 cut(s)	38
BanII	GRGCYC	1 cut(s)	2037
BbsI	GAAGAC	2 cut(s)	674, 983
Bbv12I	GWGCWC	2 cut(s)	1033, 1470
BbvI	GCAGC	5 cut(s)	64, 286, 558, 1345, 1532
BccI	CCATC	8 cut(s)	683, 933, 1079, 1183, 1198, 1537, 1780, 1951
BciT130I	CCWGG	5 cut(s)	589, 1065, 1298, 1830, 1856
BciVI	GTATCC	1 cut(s)	1260
BclI	TGATCA	1 cut(s)	895
BcoDI	GTCTC	2 cut(s)	222, 443
BfaI	CTAG	4 cut(s)	380, 822, 1134, 1164
BfmI	CTRYAG	1 cut(s)	1459
BfoI	RGCGCY	1 cut(s)	243
BfuI	GTATCC	1 cut(s)	1260
BglII	AGATCT	1 cut(s)	1707
BisI	GCNGC	7 cut(s)	78, 300, 572, 1292, 1359, 1521, 2064
BlnI	CCTAGG	1 cut(s)	1163
BlsI	GCNGC	7 cut(s)	79, 301, 573, 1293, 1360, 1522, 2065
BmcAI	AGTACT	1 cut(s)	952
Bme1390I	CCNGG	5 cut(s)	589, 1065, 1298, 1830, 1856
Bme18I	GGWCC	2 cut(s)	850, 1422
BmgT120I	GGNCC	6 cut(s)	400, 608, 850, 1088, 1422, 1936
BmiI	GGNNCC	6 cut(s)	40, 610, 852, 1062, 1295, 1938
BmrFI	CCNGG	5 cut(s)	589, 1065, 1298, 1830, 1856
BmrI	ACTGGG	1 cut(s)	1878
BmsI	GCATC	6 cut(s)	101, 451, 528, 1507, 1951, 1973
BmuI	ACTGGG	1 cut(s)	1878
BpiI	GAAGAC	2 cut(s)	674, 983
BplI	GAGNNNNNCTC	4 cut(s)	1020, 1052, 1278, 1310
BpmI	CTGGAG	6 cut(s)	93, 549, 1047, 1280, 1383, 1877
Bpu14I	TTCGAA	1 cut(s)	1657
BpuEI	CTTGAG	1 cut(s)	178
BsaJI	CCNNGG	6 cut(s)	588, 732, 1163, 1605, 1828, 1923
Bsc4I	CCNNNNNNNGG	1 cut(s)	683
Bse118I	RCCGGY	1 cut(s)	2054
Bse1I	ACTGG	5 cut(s)	76, 1375, 1501, 1582, 1884
BseBI	CCWGG	5 cut(s)	589, 1065, 1298, 1830, 1856
BseDI	CCNNGG	6 cut(s)	588, 732, 1163, 1605, 1828, 1923
BseLI	CCNNNNNNNGG	1 cut(s)	683
BseMII	CTCAG	4 cut(s)	503, 1196, 1322, 1693
BseNI	ACTGG	5 cut(s)	76, 1375, 1501, 1582, 1884
BseRI	GAGGAG	1 cut(s)	817
BseSI	GKGCMC	1 cut(s)	41
BseXI	GCAGC	5 cut(s)	64, 286, 558, 1345, 1532
BsgI	GTGCAG	1 cut(s)	1627
BshFI	GGCC	7 cut(s)	402, 609, 771, 1090, 1236, 1790, 1937
BshNI	GGYRCC	1 cut(s)	38
BsiHKAI	GWGCWC	2 cut(s)	1033, 1470
BsiSI	CCGG	1 cut(s)	2055
BslFI	GGGAC	1 cut(s)	802
BslI	CCNNNNNNNGG	1 cut(s)	683
BsmAI	GTCTC	2 cut(s)	222, 443
BsmFI	GGGAC	1 cut(s)	802
BsmI	GAATGC	3 cut(s)	1056, 1170, 1665
BsnI	GGCC	7 cut(s)	402, 609, 771, 1090, 1236, 1790, 1937
Bsp119I	TTCGAA	1 cut(s)	1657
Bsp1286I	GDGCHC	4 cut(s)	41, 1033, 1470, 2037
Bsp1407I	TGTACA	1 cut(s)	1863
Bsp143I	GATC	7 cut(s)	405, 692, 895, 1535, 1675, 1707, 1851
Bsp19I	CCATGG	1 cut(s)	1605
BspACI	CCGC	2 cut(s)	42, 1291
BspANI	GGCC	7 cut(s)	402, 609, 771, 1090, 1236, 1790, 1937
BspCNI	CTCAG	4 cut(s)	502, 1195, 1321, 1692
BspHI	TCATGA	1 cut(s)	478
BspLI	GGNNCC	6 cut(s)	40, 610, 852, 1062, 1295, 1938
BspMAI	CTGCAG	1 cut(s)	1463
BspPI	GGATC	2 cut(s)	700, 1846
BspQI	GCTCTTC	1 cut(s)	1608
BspT104I	TTCGAA	1 cut(s)	1657
BspT107I	GGYRCC	1 cut(s)	38
BsrFI	RCCGGY	1 cut(s)	2054
BsrGI	TGTACA	1 cut(s)	1863
BsrI	ACTGG	5 cut(s)	76, 1375, 1501, 1582, 1884
BssAI	RCCGGY	1 cut(s)	2054
BssECI	CCNNGG	6 cut(s)	588, 732, 1163, 1605, 1828, 1923
BssMI	GATC	7 cut(s)	405, 692, 895, 1535, 1675, 1707, 1851
BssT1I	CCWWGG	3 cut(s)	1163, 1605, 1923
Bst2UI	CCWGG	5 cut(s)	589, 1065, 1298, 1830, 1856
Bst4CI	ACNGT	3 cut(s)	167, 686, 840
Bst6I	CTCTTC	2 cut(s)	678, 1608
BstAUI	TGTACA	1 cut(s)	1863
BstBI	TTCGAA	1 cut(s)	1657
BstC8I	GCNNGC	4 cut(s)	82, 368, 388, 1122
BstDEI	CTNAG	6 cut(s)	489, 1182, 1308, 1649, 1679, 1968
BstDSI	CCRYGG	1 cut(s)	1605
BstH2I	RGCGCY	1 cut(s)	243
BstHHI	GCGC	1 cut(s)	242
BstKTI	GATC	7 cut(s)	408, 695, 898, 1538, 1678, 1710, 1854
BstMAI	GTCTC	2 cut(s)	222, 443
BstMBI	GATC	7 cut(s)	405, 692, 895, 1535, 1675, 1707, 1851
BstMWI	GCNNNNNNNGC	3 cut(s)	524, 1689, 2060
BstNI	CCWGG	5 cut(s)	589, 1065, 1298, 1830, 1856
BstNSI	RCATGY	2 cut(s)	374, 390
BstSCI	CCNGG	5 cut(s)	587, 1063, 1296, 1828, 1854
BstSFI	CTRYAG	1 cut(s)	1459
BstSLI	GKGCMC	1 cut(s)	41
BstV1I	GCAGC	5 cut(s)	64, 286, 558, 1345, 1532
BstV2I	GAAGAC	2 cut(s)	674, 983
BstX2I	RGATCY	2 cut(s)	1707, 1851
BstXI	CCANNNNNNTGG	2 cut(s)	1432, 1606
BstYI	RGATCY	2 cut(s)	1707, 1851
BsuI	GTATCC	1 cut(s)	1260
BsuRI	GGCC	7 cut(s)	402, 609, 771, 1090, 1236, 1790, 1937
BtgI	CCRYGG	1 cut(s)	1605
BtgZI	GCGATG	1 cut(s)	73
BtsI	GCAGTG	1 cut(s)	1031
BtsIMutI	CAGTG	2 cut(s)	1031, 1575
Cac8I	GCNNGC	4 cut(s)	82, 368, 388, 1122
CaiI	CAGNNNCTG	1 cut(s)	1127
CciI	TCATGA	1 cut(s)	478
CfoI	GCGC	1 cut(s)	242
Cfr10I	RCCGGY	1 cut(s)	2054
Cfr13I	GGNCC	6 cut(s)	400, 608, 850, 1088, 1422, 1936
Csp6I	GTAC	7 cut(s)	951, 991, 1437, 1513, 1717, 1864, 1873
CviQI	GTAC	7 cut(s)	951, 991, 1437, 1513, 1717, 1864, 1873
DdeI	CTNAG	6 cut(s)	489, 1182, 1308, 1649, 1679, 1968
DpnI	GATC	7 cut(s)	407, 694, 897, 1537, 1677, 1709, 1853
DpnII	GATC	7 cut(s)	405, 692, 895, 1535, 1675, 1707, 1851
EaeI	YGGCCR	1 cut(s)	1788
Eam1104I	CTCTTC	2 cut(s)	678, 1608
EarI	CTCTTC	2 cut(s)	678, 1608
Eco130I	CCWWGG	3 cut(s)	1163, 1605, 1923
Eco147I	AGGCCT	1 cut(s)	1236
Eco24I	GRGCYC	1 cut(s)	2037
Eco47I	GGWCC	2 cut(s)	850, 1422
Eco47III	AGCGCT	1 cut(s)	241
Eco57I	CTGAAG	2 cut(s)	1217, 2010
EcoRII	CCWGG	5 cut(s)	587, 1063, 1296, 1828, 1854
EcoT14I	CCWWGG	3 cut(s)	1163, 1605, 1923
EcoT22I	ATGCAT	1 cut(s)	1966
EcoT38I	GRGCYC	1 cut(s)	2037
ErhI	CCWWGG	3 cut(s)	1163, 1605, 1923
FalI	AAGNNNNNCTT	2 cut(s)	1784, 1816
FaqI	GGGAC	1 cut(s)	802
FauNDI	CATATG	2 cut(s)	553, 565
FbaI	TGATCA	1 cut(s)	895
FblI	GTMKAC	1 cut(s)	777
Fnu4HI	GCNGC	7 cut(s)	78, 300, 572, 1292, 1359, 1521, 2064
FriOI	GRGCYC	1 cut(s)	2037
Fsp4HI	GCNGC	7 cut(s)	78, 300, 572, 1292, 1359, 1521, 2064
FspBI	CTAG	4 cut(s)	380, 822, 1134, 1164
GlaI	GCGC	1 cut(s)	241
GluI	GCNGC	7 cut(s)	78, 300, 572, 1292, 1359, 1521, 2064
GsuI	CTGGAG	6 cut(s)	93, 549, 1047, 1280, 1383, 1877
HaeII	RGCGCY	1 cut(s)	243
HaeIII	GGCC	7 cut(s)	402, 609, 771, 1090, 1236, 1790, 1937
HapII	CCGG	1 cut(s)	2055
HhaI	GCGC	1 cut(s)	242
Hin6I	GCGC	1 cut(s)	240
HinP1I	GCGC	1 cut(s)	240
HincII	GTYRAC	2 cut(s)	253, 316
HindII	GTYRAC	2 cut(s)	253, 316
HindIII	AAGCTT	2 cut(s)	155, 923
HpaII	CCGG	1 cut(s)	2055
HphI	GGTGA	3 cut(s)	259, 698, 773
Hpy166II	GTNNAC	4 cut(s)	253, 316, 345, 778
Hpy8I	GTNNAC	4 cut(s)	253, 316, 345, 778
HpyAV	CCTTC	5 cut(s)	194, 272, 959, 1247, 1798
HpyCH4III	ACNGT	3 cut(s)	167, 686, 840
HpyCH4IV	ACGT	4 cut(s)	559, 993, 1303, 2025
HpyCH4V	TGCA	7 cut(s)	105, 292, 390, 1461, 1520, 1644, 1964
HpyF10VI	GCNNNNNNNGC	3 cut(s)	524, 1689, 2060
HpyF3I	CTNAG	6 cut(s)	489, 1182, 1308, 1649, 1679, 1968
HpySE526I	ACGT	4 cut(s)	559, 993, 1303, 2025
HspAI	GCGC	1 cut(s)	240
Ksp22I	TGATCA	1 cut(s)	895
Kzo9I	GATC	7 cut(s)	405, 692, 895, 1535, 1675, 1707, 1851
LguI	GCTCTTC	1 cut(s)	1608
LmnI	GCTCC	7 cut(s)	74, 530, 1028, 1041, 1165, 1299, 2060
Lsp1109I	GCAGC	5 cut(s)	64, 286, 558, 1345, 1532
LweI	GCATC	6 cut(s)	101, 451, 528, 1507, 1951, 1973
MaeI	CTAG	4 cut(s)	380, 822, 1134, 1164
MaeII	ACGT	4 cut(s)	559, 993, 1303, 2025
MaeIII	GTNAC	5 cut(s)	329, 394, 1366, 1841, 1946
MalI	GATC	7 cut(s)	407, 694, 897, 1537, 1677, 1709, 1853
MboI	GATC	7 cut(s)	405, 692, 895, 1535, 1675, 1707, 1851
MflI	RGATCY	2 cut(s)	1707, 1851
MhlI	GDGCHC	4 cut(s)	41, 1033, 1470, 2037
MlsI	TGGCCA	1 cut(s)	1790
MluNI	TGGCCA	1 cut(s)	1790
MlyI	GAGTC	5 cut(s)	14, 281, 664, 1849, 2018
MmeI	TCCRAC	1 cut(s)	828
Mox20I	TGGCCA	1 cut(s)	1790
Mph1103I	ATGCAT	1 cut(s)	1966
MroXI	GAANNNNTTC	2 cut(s)	1416, 1982
MscI	TGGCCA	1 cut(s)	1790
MseI	TTAA	7 cut(s)	891, 927, 1335, 1478, 1899, 2019, 2071
MslI	CAYNNNNRTG	2 cut(s)	687, 759
Msp20I	TGGCCA	1 cut(s)	1790
MspI	CCGG	1 cut(s)	2055
MspR9I	CCNGG	5 cut(s)	589, 1065, 1298, 1830, 1856
Mva1269I	GAATGC	3 cut(s)	1056, 1170, 1665
MvaI	CCWGG	5 cut(s)	589, 1065, 1298, 1830, 1856
MwoI	GCNNNNNNNGC	3 cut(s)	524, 1689, 2060
NcoI	CCATGG	1 cut(s)	1605
NdeI	CATATG	2 cut(s)	553, 565
NdeII	GATC	7 cut(s)	405, 692, 895, 1535, 1675, 1707, 1851
NlaIV	GGNNCC	6 cut(s)	40, 610, 852, 1062, 1295, 1938
NmeAIII	GCCGAG	1 cut(s)	757
NmuCI	GTSAC	3 cut(s)	394, 1366, 1841
NsiI	ATGCAT	1 cut(s)	1966
NspI	RCATGY	2 cut(s)	374, 390
NspV	TTCGAA	1 cut(s)	1657
PaeI	GCATGC	1 cut(s)	390
PagI	TCATGA	1 cut(s)	478
PceI	AGGCCT	1 cut(s)	1236
PciSI	GCTCTTC	1 cut(s)	1608
PctI	GAATGC	3 cut(s)	1056, 1170, 1665
PdmI	GAANNNNTTC	2 cut(s)	1416, 1982
PfeI	GAWTC	6 cut(s)	634, 679, 1000, 1109, 1130, 1397
PflFI	GACNNNGTC	2 cut(s)	248, 1637
PfoI	TCCNGGA	3 cut(s)	1063, 1296, 1854
PkrI	GCNGC	7 cut(s)	79, 301, 573, 1293, 1360, 1522, 2065
PleI	GAGTC	5 cut(s)	13, 280, 664, 1848, 2017
PpsI	GAGTC	5 cut(s)	13, 280, 664, 1848, 2017
Psp1406I	AACGTT	1 cut(s)	1303
Psp6I	CCWGG	5 cut(s)	587, 1063, 1296, 1828, 1854
PspGI	CCWGG	5 cut(s)	587, 1063, 1296, 1828, 1854
PspN4I	GGNNCC	6 cut(s)	40, 610, 852, 1062, 1295, 1938
PspPI	GGNCC	6 cut(s)	400, 608, 850, 1088, 1422, 1936
PstI	CTGCAG	1 cut(s)	1463
PstNI	CAGNNNCTG	1 cut(s)	1127
PsuI	RGATCY	2 cut(s)	1707, 1851
PsyI	GACNNNGTC	2 cut(s)	248, 1637
RsaI	GTAC	7 cut(s)	952, 992, 1438, 1514, 1718, 1865, 1874
RsaNI	GTAC	7 cut(s)	951, 991, 1437, 1513, 1717, 1864, 1873
RseI	CAYNNNNRTG	2 cut(s)	687, 759
SapI	GCTCTTC	1 cut(s)	1608
SaqAI	TTAA	7 cut(s)	891, 927, 1335, 1478, 1899, 2019, 2071
SatI	GCNGC	7 cut(s)	78, 300, 572, 1292, 1359, 1521, 2064
Sau3AI	GATC	7 cut(s)	405, 692, 895, 1535, 1675, 1707, 1851
Sau96I	GGNCC	6 cut(s)	400, 608, 850, 1088, 1422, 1936
ScaI	AGTACT	1 cut(s)	952
SchI	GAGTC	5 cut(s)	14, 281, 664, 1849, 2018
ScrFI	CCNGG	5 cut(s)	589, 1065, 1298, 1830, 1856
SduI	GDGCHC	4 cut(s)	41, 1033, 1470, 2037
SfaNI	GCATC	6 cut(s)	101, 451, 528, 1507, 1951, 1973
SfcI	CTRYAG	1 cut(s)	1459
SfuI	TTCGAA	1 cut(s)	1657
SinI	GGWCC	2 cut(s)	850, 1422
SmiMI	CAYNNNNRTG	2 cut(s)	687, 759
SmlI	CTYRAG	1 cut(s)	193
SmoI	CTYRAG	1 cut(s)	193
SphI	GCATGC	1 cut(s)	390
SseBI	AGGCCT	1 cut(s)	1236
SsiI	CCGC	2 cut(s)	42, 1291
SspMI	CTAG	4 cut(s)	380, 822, 1134, 1164
StuI	AGGCCT	1 cut(s)	1236
StyD4I	CCNGG	5 cut(s)	587, 1063, 1296, 1828, 1854
StyI	CCWWGG	3 cut(s)	1163, 1605, 1923
TaaI	ACNGT	3 cut(s)	167, 686, 840
TaiI	ACGT	4 cut(s)	562, 996, 1306, 2028
TaqI	TCGA	2 cut(s)	1285, 1657
TatI	WGTACW	4 cut(s)	950, 1716, 1863, 1872
TauI	GCSGC	1 cut(s)	1294
TfiI	GAWTC	6 cut(s)	634, 679, 1000, 1109, 1130, 1397
Tru1I	TTAA	7 cut(s)	891, 927, 1335, 1478, 1899, 2019, 2071
Tru9I	TTAA	7 cut(s)	891, 927, 1335, 1478, 1899, 2019, 2071
TscAI	CASTG	2 cut(s)	1038, 1582
TseFI	GTSAC	3 cut(s)	394, 1366, 1841
TseI	GCWGC	6 cut(s)	77, 299, 571, 1358, 1520, 2063
Tsp45I	GTSAC	3 cut(s)	394, 1366, 1841
TspRI	CASTG	2 cut(s)	1038, 1582
Tth111I	GACNNNGTC	2 cut(s)	248, 1637
VpaK11BI	GGWCC	2 cut(s)	850, 1422
XapI	RAATTY	4 cut(s)	172, 215, 828, 1570
XbaI	TCTAGA	1 cut(s)	379
XceI	RCATGY	2 cut(s)	374, 390
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1606, 1930
XmaJI	CCTAGG	1 cut(s)	1163
XmiI	GTMKAC	1 cut(s)	777
XmnI	GAANNNNTTC	2 cut(s)	1416, 1982
XspI	CTAG	4 cut(s)	380, 822, 1134, 1164
ZrmI	AGTACT	1 cut(s)	952
Zsp2I	ATGCAT	1 cut(s)	1966

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.