MD15G1106300.v1.1

rhamnose biosynthetic enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0002188
Physical Location & Seq
Forward (+)
7428200 .. 7430360
2161 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1106300.v1.1.491

Sequence Viewer

Length: 1983 bp
ATGGAATCTGAAGGTACACCATATGCACCAAAGAGCATCCTCATAACAGGAGCAGCAGGATTCATAGGCAGCCACGTGACCAATCGCCTAATAAAAAACTATCCCAGCTACAAAATCGTTGCTCTTGACAAGATAGACTACTGCTCTAGCTTCAAGAACCTTAGGCCTTGTCGTTCCTCCCCGAATTTCAAGTTTGTCAAGGGAGACATTGCCTGTGCTGATCTCATAAACCATCTCCTGATTGCCGATGAGATCGACACCATAATGCACTTCGCCGCACAAACCCACGTGGACAATTCGTTCGGAAACTCGTTCGAGTTCACCAACAACAATGTCTACGGCACTCACGTCCTCCTCGAAGCATGCAAGGTCACGCAGCGAGTGAAGCGCTTCATTCATGTGAGTACTGATGAAGTTTATGGAGAGACGGATATGGAGACCAATATTGGAAACCCTGAGGCCTCTCAGCTCCTCCCTACAAACCCTTACTCAGCCACAAAAGCTGGGGCTGAAATGTTGGTCATGGCATACCACAGATCCTACGGTTTGCCAACAATAACAACTAGAAGCAACAATGTGTACGGACCCCACCAGTATCCAGAAAAGCTTATCCCAAAATTTAGCCTTCTTGCAATGAAAGGTGAGAAATTACCAATCCATGGGAATGGATCAAATGTGAGAAGCTATTTGTATTGTGATGACGTGGCTGAGGCATTTGATGTGATACTTCACAAGGGAGTGATAGGGCATGTTTACAACATTGGCACCAAAAAAGAGAGGTCAGTTTTGAACGTGGCAGAAGATATTTGCAAAATGATTGGTTTGAATTCAAAGGAAGCTATAACTTTTGTGCAAGATAGGCCTTTCAATGACCAAAGGTACTTTTTGGATGATCAAAAATTGAAAAGGCTAGGGTGGGATGTGAGGACTAGTTGGGAGGAAGGGCTGAAGCTGACGACAGAATGGTACACGAAGCACGCGGATTGGTGGGGTGATGTGTCTGCTGCACTTCACCCACACCCAAGTTTCGCAGTGATTAGCCGACCGAATGATGATTCTTGGTTCTTCGAATATGGGTTCACAAGGCTGTCGAGAACATGCAATGAGGGTAGCAATAGTTCTGAATTGAAGTTTTTGATATATGGAAGGACTGGATGGATTGGAGGGTTGTTGGGGAAGCTTTGTAAGGGTGAAGGAATTGAGTTTGAGTATGGGAAAGGACGACTTGAGGATAGGAAGTCTCTGCTGGAGGACATCACAAGAGTACAACCAACCCATGTGTTCAATGCTGCTGGAGTTACTGGGCGGCCAAACGTTGATTGGTGTGAATCTCACAAGGCGCAAACAATTCGGACTAATGTAGCTGGCACGCTTAATTTGGCTGATGTTTGCAAGGATCAAGGTCTGTTGATGATGAATTTTGCGACCGGATGCATTTTTGAGTATGACAAGGAGCATCCCCTAGGATCTGGTATTGGATTCAAGGAGGAGGACAACCCTAATTTCACCGGTTCCTTCTATTCGAAGACCAAAGCCATGGTGGAGGAGCTGTTGAAGGAATATGACAAGGTGTGCACCCTCAGAGTGAGAATGCCAATATCATCAGACCTCAGCAACCCTAGAAACTTCATCACAAAGATAGCGCGCAACGACAAGGTTGTGAACATTCCCAACAGCATGACAGTCCTAGATGAGCTCCTACCGATCTCCATTGAGATGGCTCGGAGGAACTGCAGAGGAATATGGAACTTCACCAACCCAGGAGTCATAAGCCACAACGAGATCCTAGAGATGTACAGGGATTACATTGATCCAAAGTTCAAGTGGCAAAATTTTGACCTAGAAGAACAGGCCAAGGTGATTGTCACGCCAAGGAGCAACAACGAATTGGATGCTTCAAAGCTGAAGAAAGAGTTCCCCGAATTGCTTTCGATCAAAGATTCAATCATAAAATATGTCTTTGAGCCCATCAAGAAGACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00520 map00520
Pfam Domains
Protein Families
Epimerase GDP_Man_Dehyd

Protein Analysis

661

Amino Acids

74.79

Weight (kDa)

6.55

Isoelectric Point (pI)

28.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RmlD_sub_bind PF04321 12 - 319 2e-15 RmlD substrate binding domain
Epimerase PF01370 13 - 255 1.8e-54 NAD dependent epimerase/dehydratase family
Polysacc_synt_2 PF02719 13 - 279 3.8e-18 Polysaccharide biosynthesis protein
GDP_Man_Dehyd PF16363 14 - 317 4.1e-66 GDP-mannose 4,6 dehydratase
3Beta_HSD PF01073 14 - 239 3.2e-24 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_4 PF07993 15 - 197 3.6e-18 Male sterility protein
RmlD_sub_bind PF04321 377 - 549 1.7e-12 RmlD substrate binding domain
Epimerase PF01370 382 - 541 8e-13 NAD dependent epimerase/dehydratase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 764
AccB7I CCANNNNNTGG 1 cut(s) 659
AccI GTMKAC 1 cut(s) 336
AccII CGCG 2 cut(s) 980, 1645
AciI CCGC 3 cut(s) 276, 980, 1306
AclI AACGTT 1 cut(s) 1314
AclWI GGATC 6 cut(s) 531, 676, 1404, 1474, 1777, 1805
AcoI YGGCCR 1 cut(s) 1307
AcsI RAATTY 5 cut(s) 184, 617, 826, 1417, 1831
AcuI CTGAAG 3 cut(s) 30, 968, 1925
AcvI CACGTG 2 cut(s) 76, 289
AfaI GTAC 7 cut(s) 16, 406, 581, 881, 968, 1266, 1796
AfeI AGCGCT 1 cut(s) 389
AfiI CCNNNNNNNGG 1 cut(s) 659
AgeI ACCGGT 1 cut(s) 1508
AhlI ACTAGT 1 cut(s) 929
AjiI CACGTC 2 cut(s) 349, 703
AjnI CCWGG 1 cut(s) 1759
AloI GAACNNNNNNTCC 2 cut(s) 284, 316
Alw21I GWGCWC 2 cut(s) 1577, 1698
Alw26I GTCTC 4 cut(s) 198, 419, 431, 1245
Alw44I GTGCAC 1 cut(s) 1573
AlwI GGATC 6 cut(s) 531, 676, 1404, 1474, 1777, 1805
Aor51HI AGCGCT 1 cut(s) 389
AoxI GGCC 5 cut(s) 164, 459, 860, 1307, 1851
ApaLI GTGCAC 1 cut(s) 1573
ApeKI GCWGC 5 cut(s) 53, 69, 376, 1004, 1289
ApoI RAATTY 5 cut(s) 184, 617, 826, 1417, 1831
ArsI GACNNNNNNTTYG 2 cut(s) 284, 316
AsiGI ACCGGT 1 cut(s) 1508
Asp700I GAANNNNTTC 2 cut(s) 389, 1913
AspA2I CCTAGG 1 cut(s) 1462
AspLEI GCGC 4 cut(s) 390, 1342, 1645, 1647
AspS9I GGNCC 1 cut(s) 584
AsuHPI GGTGA 8 cut(s) 313, 653, 1004, 1004, 1202, 1498, 1744, 1870
AsuII TTCGAA 2 cut(s) 1068, 1523
AvaII GGWCC 1 cut(s) 584
AvrII CCTAGG 1 cut(s) 1462
AxyI CCTNAGG 2 cut(s) 161, 456
BaeGI GKGCMC 1 cut(s) 1577
BanI GGYRCC 1 cut(s) 764
BanII GRGCYC 2 cut(s) 1698, 1968
BbrPI CACGTG 2 cut(s) 76, 289
BbsI GAAGAC 1 cut(s) 1532
Bbv12I GWGCWC 2 cut(s) 1577, 1698
BbvCI CCTCAGC 2 cut(s) 708, 1610
BbvI GCAGC 5 cut(s) 65, 81, 388, 991, 1276
BccI CCATC 4 cut(s) 240, 1149, 1711, 1976
BceAI ACGGC 1 cut(s) 355
BcgI CGANNNNNNTGC 4 cut(s) 1331, 1365, 1874, 1908
BciT130I CCWGG 1 cut(s) 1761
BciVI GTATCC 1 cut(s) 606
BclI TGATCA 1 cut(s) 892
BcoDI GTCTC 4 cut(s) 198, 419, 431, 1245
BcuI ACTAGT 1 cut(s) 929
BfaI CTAG 9 cut(s) 147, 564, 911, 930, 1463, 1620, 1688, 1787, 1841
BfmI CTRYAG 1 cut(s) 1732
BfoI RGCGCY 1 cut(s) 391
BfuI GTATCC 1 cut(s) 606
BisI GCNGC 7 cut(s) 54, 70, 276, 377, 1005, 1290, 1307
BlnI CCTAGG 1 cut(s) 1462
BlsI GCNGC 7 cut(s) 55, 71, 277, 378, 1006, 1291, 1308
BmcAI AGTACT 1 cut(s) 406
Bme1390I CCNGG 1 cut(s) 1761
Bme18I GGWCC 1 cut(s) 584
BmgBI CACGTC 2 cut(s) 349, 703
BmgT120I GGNCC 1 cut(s) 584
BmiI GGNNCC 3 cut(s) 586, 766, 1513
BmrFI CCNGG 1 cut(s) 1761
BmrI ACTGGG 1 cut(s) 1311
BmsI GCATC 4 cut(s) 45, 1421, 1465, 1882
BmuI ACTGGG 1 cut(s) 1311
BpiI GAAGAC 1 cut(s) 1532
BpmI CTGGAG 2 cut(s) 1268, 1314
Bpu10I CCTNAGC 2 cut(s) 708, 1610
Bpu14I TTCGAA 2 cut(s) 1068, 1523
BpuEI CTTGAG 1 cut(s) 1247
BsaAI YACGTR 2 cut(s) 76, 289
BsaI GGTCTC 1 cut(s) 431
BsaJI CCNNGG 6 cut(s) 658, 1462, 1536, 1759, 1854, 1871
BsaWI WCCGGW 2 cut(s) 1427, 1508
BsaXI ACNNNNNCTCC 2 cut(s) 1535, 1565
Bsc4I CCNNNNNNNGG 1 cut(s) 659
Bse118I RCCGGY 1 cut(s) 1508
Bse1I ACTGG 3 cut(s) 592, 1156, 1306
Bse21I CCTNAGG 2 cut(s) 161, 456
Bse3DI GCAATG 3 cut(s) 207, 639, 1108
BseBI CCWGG 1 cut(s) 1761
BseDI CCNNGG 6 cut(s) 658, 1462, 1536, 1759, 1854, 1871
BseGI GGATG 7 cut(s) 36, 895, 925, 1160, 1436, 1456, 1897
BseLI CCNNNNNNNGG 1 cut(s) 659
BseMI GCAATG 3 cut(s) 207, 639, 1108
BseMII CTCAG 6 cut(s) 447, 479, 504, 699, 1594, 1624
BseNI ACTGG 3 cut(s) 592, 1156, 1306
BsePI GCGCGC 1 cut(s) 1643
BseRI GAGGAG 4 cut(s) 344, 461, 1502, 1559
BseSI GKGCMC 1 cut(s) 1577
BseXI GCAGC 5 cut(s) 65, 81, 388, 991, 1276
BseYI CCCAGC 2 cut(s) 104, 503
BsgI GTGCAG 1 cut(s) 990
Bsh1236I CGCG 2 cut(s) 980, 1645
Bsh1285I CGRYCG 2 cut(s) 1046, 1428
BshFI GGCC 5 cut(s) 166, 461, 862, 1309, 1853
BshNI GGYRCC 1 cut(s) 764
BshTI ACCGGT 1 cut(s) 1508
BsiEI CGRYCG 2 cut(s) 1046, 1428
BsiHKAI GWGCWC 2 cut(s) 1577, 1698
BsiSI CCGG 2 cut(s) 1428, 1509
BslI CCNNNNNNNGG 1 cut(s) 659
BsmAI GTCTC 4 cut(s) 198, 419, 431, 1245
BsmBI CGTCTC 1 cut(s) 419
BsmI GAATGC 1 cut(s) 1596
BsnI GGCC 5 cut(s) 166, 461, 862, 1309, 1853
Bso31I GGTCTC 1 cut(s) 431
Bsp119I TTCGAA 2 cut(s) 1068, 1523
Bsp1286I GDGCHC 3 cut(s) 1577, 1698, 1968
Bsp1407I TGTACA 1 cut(s) 1794
Bsp19I CCATGG 2 cut(s) 658, 1536
BspACI CCGC 3 cut(s) 276, 980, 1306
BspANI GGCC 5 cut(s) 166, 461, 862, 1309, 1853
BspCNI CTCAG 6 cut(s) 448, 478, 503, 700, 1593, 1623
BspFNI CGCG 2 cut(s) 980, 1645
BspLI GGNNCC 3 cut(s) 586, 766, 1513
BspMAI CTGCAG 1 cut(s) 1736
BspPI GGATC 6 cut(s) 531, 676, 1404, 1474, 1777, 1805
BspT104I TTCGAA 2 cut(s) 1068, 1523
BspT107I GGYRCC 1 cut(s) 764
BspTNI GGTCTC 1 cut(s) 431
BsrDI GCAATG 3 cut(s) 207, 639, 1108
BsrFI RCCGGY 1 cut(s) 1508
BsrGI TGTACA 1 cut(s) 1794
BsrI ACTGG 3 cut(s) 592, 1156, 1306
BssAI RCCGGY 1 cut(s) 1508
BssECI CCNNGG 6 cut(s) 658, 1462, 1536, 1759, 1854, 1871
BssHII GCGCGC 1 cut(s) 1643
BssT1I CCWWGG 5 cut(s) 658, 1462, 1536, 1854, 1871
Bst2UI CCWGG 1 cut(s) 1761
Bst4CI ACNGT 2 cut(s) 545, 1684
BstAUI TGTACA 1 cut(s) 1794
BstBAI YACGTR 2 cut(s) 76, 289
BstBI TTCGAA 2 cut(s) 1068, 1523
BstC8I GCNNGC 5 cut(s) 364, 978, 1366, 1370, 1645
BstDEI CTNAG 7 cut(s) 161, 456, 465, 490, 708, 1580, 1610
BstDSI CCRYGG 2 cut(s) 658, 1536
BstF5I GGATG 7 cut(s) 36, 895, 925, 1160, 1436, 1456, 1897
BstFNI CGCG 2 cut(s) 980, 1645
BstH2I RGCGCY 1 cut(s) 391
BstHHI GCGC 4 cut(s) 390, 1342, 1645, 1647
BstMAI GTCTC 4 cut(s) 198, 419, 431, 1245
BstMCI CGRYCG 2 cut(s) 1046, 1428
BstMWI GCNNNNNNNGC 3 cut(s) 385, 500, 859
BstNI CCWGG 1 cut(s) 1761
BstNSI RCATGY 3 cut(s) 366, 752, 1101
BstSCI CCNGG 1 cut(s) 1759
BstSFI CTRYAG 1 cut(s) 1732
BstSLI GKGCMC 1 cut(s) 1577
BstUI CGCG 2 cut(s) 980, 1645
BstV1I GCAGC 5 cut(s) 65, 81, 388, 991, 1276
BstV2I GAAGAC 1 cut(s) 1532
BstX2I RGATCY 3 cut(s) 536, 1466, 1782
BstXI CCANNNNNNTGG 3 cut(s) 665, 1537, 1717
BstYI RGATCY 3 cut(s) 536, 1466, 1782
Bsu36I CCTNAGG 2 cut(s) 161, 456
BsuI GTATCC 1 cut(s) 606
BsuRI GGCC 5 cut(s) 166, 461, 862, 1309, 1853
BtgI CCRYGG 2 cut(s) 658, 1536
BtrI CACGTC 2 cut(s) 349, 703
BtsCI GGATG 7 cut(s) 36, 895, 925, 1160, 1436, 1456, 1897
BtsI GCAGTG 1 cut(s) 1038
BtsIMutI CAGTG 1 cut(s) 1038
Cac8I GCNNGC 5 cut(s) 364, 978, 1366, 1370, 1645
CfoI GCGC 4 cut(s) 390, 1342, 1645, 1647
Cfr10I RCCGGY 1 cut(s) 1508
Cfr13I GGNCC 1 cut(s) 584
Csp6I GTAC 7 cut(s) 15, 405, 580, 880, 967, 1265, 1795
CspAI ACCGGT 1 cut(s) 1508
CviAII CATG 9 cut(s) 363, 398, 523, 659, 749, 1098, 1277, 1537, 1678
CviQI GTAC 7 cut(s) 15, 405, 580, 880, 967, 1265, 1795
DdeI CTNAG 7 cut(s) 161, 456, 465, 490, 708, 1580, 1610
EaeI YGGCCR 1 cut(s) 1307
Ecl136II GAGCTC 1 cut(s) 1696
Eco130I CCWWGG 5 cut(s) 658, 1462, 1536, 1854, 1871
Eco147I AGGCCT 3 cut(s) 166, 461, 862
Eco24I GRGCYC 2 cut(s) 1698, 1968
Eco31I GGTCTC 1 cut(s) 431
Eco47I GGWCC 1 cut(s) 584
Eco47III AGCGCT 1 cut(s) 389
Eco53kI GAGCTC 1 cut(s) 1696
Eco57I CTGAAG 3 cut(s) 30, 968, 1925
Eco72I CACGTG 2 cut(s) 76, 289
Eco81I CCTNAGG 2 cut(s) 161, 456
EcoICRI GAGCTC 1 cut(s) 1696
EcoRI GAATTC 1 cut(s) 826
EcoRII CCWGG 1 cut(s) 1759
EcoT14I CCWWGG 5 cut(s) 658, 1462, 1536, 1854, 1871
EcoT22I ATGCAT 1 cut(s) 1436
EcoT38I GRGCYC 2 cut(s) 1698, 1968
ErhI CCWWGG 5 cut(s) 658, 1462, 1536, 1854, 1871
Esp3I CGTCTC 1 cut(s) 419
FaeI CATG 9 cut(s) 366, 401, 526, 662, 752, 1101, 1280, 1540, 1681
FalI AAGNNNNNCTT 2 cut(s) 1209, 1241
FatI CATG 9 cut(s) 362, 397, 522, 658, 748, 1097, 1276, 1536, 1677
FauNDI CATATG 1 cut(s) 22
FbaI TGATCA 1 cut(s) 892
FblI GTMKAC 1 cut(s) 336
Fnu4HI GCNGC 7 cut(s) 54, 70, 276, 377, 1005, 1290, 1307
FokI GGATG 7 cut(s) 23, 902, 932, 1167, 1443, 1443, 1904
FriOI GRGCYC 2 cut(s) 1698, 1968
Fsp4HI GCNGC 7 cut(s) 54, 70, 276, 377, 1005, 1290, 1307
FspBI CTAG 9 cut(s) 147, 564, 911, 930, 1463, 1620, 1688, 1787, 1841
GlaI GCGC 4 cut(s) 389, 1341, 1644, 1646
GluI GCNGC 7 cut(s) 54, 70, 276, 377, 1005, 1290, 1307
GsaI CCCAGC 2 cut(s) 108, 507
GsuI CTGGAG 2 cut(s) 1268, 1314
HaeII RGCGCY 1 cut(s) 391
HaeIII GGCC 5 cut(s) 166, 461, 862, 1309, 1853
HapII CCGG 2 cut(s) 1428, 1509
HhaI GCGC 4 cut(s) 390, 1342, 1645, 1647
Hin1II CATG 9 cut(s) 366, 401, 526, 662, 752, 1101, 1280, 1540, 1681
Hin6I GCGC 4 cut(s) 388, 1340, 1643, 1645
HinP1I GCGC 4 cut(s) 388, 1340, 1643, 1645
HindIII AAGCTT 2 cut(s) 605, 1178
HinfI GANTC 7 cut(s) 5, 60, 1055, 1328, 1479, 1764, 1940
HpaII CCGG 2 cut(s) 1428, 1509
HphI GGTGA 8 cut(s) 313, 653, 1004, 1004, 1202, 1498, 1744, 1870
Hpy188I TCNGA 7 cut(s) 10, 305, 1123, 1353, 1583, 1606, 1725
Hpy188III TCNNGA 6 cut(s) 125, 154, 238, 599, 1092, 1972
HpyAV CCTTC 7 cut(s) 5, 635, 935, 1140, 1187, 1525, 1549
HpyCH4III ACNGT 2 cut(s) 545, 1684
HpyCH4IV ACGT 6 cut(s) 75, 288, 348, 702, 792, 1314
HpyF10VI GCNNNNNNNGC 3 cut(s) 385, 500, 859
HpyF3I CTNAG 7 cut(s) 161, 456, 465, 490, 708, 1580, 1610
HpySE526I ACGT 6 cut(s) 75, 288, 348, 702, 792, 1314
Hsp92II CATG 9 cut(s) 366, 401, 526, 662, 752, 1101, 1280, 1540, 1681
HspAI GCGC 4 cut(s) 388, 1340, 1643, 1645
Ksp22I TGATCA 1 cut(s) 892
LmnI GCTCC 6 cut(s) 50, 474, 1453, 1546, 1701, 1875
Lsp1109I GCAGC 5 cut(s) 65, 81, 388, 991, 1276
LweI GCATC 4 cut(s) 45, 1421, 1465, 1882
MaeI CTAG 9 cut(s) 147, 564, 911, 930, 1463, 1620, 1688, 1787, 1841
MaeII ACGT 6 cut(s) 75, 288, 348, 702, 792, 1314
MaeIII GTNAC 4 cut(s) 76, 370, 1297, 1864
MboII GAAGA 5 cut(s) 812, 1057, 1537, 1856, 1918
MflI RGATCY 3 cut(s) 536, 1466, 1782
MhlI GDGCHC 3 cut(s) 1577, 1698, 1968
MlyI GAGTC 1 cut(s) 1773
Mph1103I ATGCAT 1 cut(s) 1436
MroXI GAANNNNTTC 2 cut(s) 389, 1913
MseI TTAA 1 cut(s) 1374
MslI CAYNNNNRTG 4 cut(s) 263, 398, 663, 1715
MspI CCGG 2 cut(s) 1428, 1509
MspR9I CCNGG 1 cut(s) 1761
Mva1269I GAATGC 1 cut(s) 1596
MvaI CCWGG 1 cut(s) 1761
MvnI CGCG 2 cut(s) 980, 1645
MwoI GCNNNNNNNGC 3 cut(s) 385, 500, 859
NcoI CCATGG 2 cut(s) 658, 1536
NdeI CATATG 1 cut(s) 22
NlaIII CATG 9 cut(s) 366, 401, 526, 662, 752, 1101, 1280, 1540, 1681
NlaIV GGNNCC 3 cut(s) 586, 766, 1513
NmuCI GTSAC 3 cut(s) 76, 370, 1864
NsiI ATGCAT 1 cut(s) 1436
NspI RCATGY 3 cut(s) 366, 752, 1101
NspV TTCGAA 2 cut(s) 1068, 1523
PaeI GCATGC 1 cut(s) 366
PauI GCGCGC 1 cut(s) 1643
PceI AGGCCT 3 cut(s) 166, 461, 862
PcsI WCGNNNNNNNCGW 2 cut(s) 345, 354
PctI GAATGC 1 cut(s) 1596
PdmI GAANNNNTTC 2 cut(s) 389, 1913
PfeI GAWTC 6 cut(s) 5, 60, 1055, 1328, 1479, 1940
PflMI CCANNNNNTGG 1 cut(s) 659
PinAI ACCGGT 1 cut(s) 1508
PkrI GCNGC 7 cut(s) 55, 71, 277, 378, 1006, 1291, 1308
PleI GAGTC 1 cut(s) 1772
PmaCI CACGTG 2 cut(s) 76, 289
PmlI CACGTG 2 cut(s) 76, 289
PpsI GAGTC 1 cut(s) 1772
Ppu21I YACGTR 2 cut(s) 76, 289
Psp124BI GAGCTC 1 cut(s) 1698
Psp1406I AACGTT 1 cut(s) 1314
Psp6I CCWGG 1 cut(s) 1759
PspCI CACGTG 2 cut(s) 76, 289
PspFI CCCAGC 2 cut(s) 104, 503
PspGI CCWGG 1 cut(s) 1759
PspN4I GGNNCC 3 cut(s) 586, 766, 1513
PspPI GGNCC 1 cut(s) 584
PsrI GAACNNNNNNTAC 2 cut(s) 1102, 1134
PstI CTGCAG 1 cut(s) 1736
PsuI RGATCY 3 cut(s) 536, 1466, 1782
PteI GCGCGC 1 cut(s) 1643
RsaI GTAC 7 cut(s) 16, 406, 581, 881, 968, 1266, 1796
RsaNI GTAC 7 cut(s) 15, 405, 580, 880, 967, 1265, 1795
RseI CAYNNNNRTG 4 cut(s) 263, 398, 663, 1715
SacI GAGCTC 1 cut(s) 1698
SaqAI TTAA 1 cut(s) 1374
SatI GCNGC 7 cut(s) 54, 70, 276, 377, 1005, 1290, 1307
Sau96I GGNCC 1 cut(s) 584
ScaI AGTACT 1 cut(s) 406
SchI GAGTC 1 cut(s) 1773
ScrFI CCNGG 1 cut(s) 1761
SduI GDGCHC 3 cut(s) 1577, 1698, 1968
SfaNI GCATC 4 cut(s) 45, 1421, 1465, 1882
SfcI CTRYAG 1 cut(s) 1732
SfuI TTCGAA 2 cut(s) 1068, 1523
SinI GGWCC 1 cut(s) 584
SmiMI CAYNNNNRTG 4 cut(s) 263, 398, 663, 1715
SmlI CTYRAG 1 cut(s) 1226
SmoI CTYRAG 1 cut(s) 1226
SpeI ACTAGT 1 cut(s) 929
SphI GCATGC 1 cut(s) 366
SseBI AGGCCT 3 cut(s) 166, 461, 862
SsiI CCGC 3 cut(s) 276, 980, 1306
SspI AATATT 1 cut(s) 445
SspMI CTAG 9 cut(s) 147, 564, 911, 930, 1463, 1620, 1688, 1787, 1841
SstI GAGCTC 1 cut(s) 1698
StuI AGGCCT 3 cut(s) 166, 461, 862
StyD4I CCNGG 1 cut(s) 1759
StyI CCWWGG 5 cut(s) 658, 1462, 1536, 1854, 1871
TaaI ACNGT 2 cut(s) 545, 1684
TaiI ACGT 6 cut(s) 78, 291, 351, 705, 795, 1317
TaqI TCGA 7 cut(s) 255, 315, 357, 1068, 1091, 1523, 1931
TaqII GACCGA 1 cut(s) 1060
TatI WGTACW 3 cut(s) 404, 1264, 1794
TauI GCSGC 2 cut(s) 278, 1309
TfiI GAWTC 6 cut(s) 5, 60, 1055, 1328, 1479, 1940
Tru1I TTAA 1 cut(s) 1374
Tru9I TTAA 1 cut(s) 1374
TscAI CASTG 1 cut(s) 1038
TseFI GTSAC 3 cut(s) 76, 370, 1864
TseI GCWGC 5 cut(s) 53, 69, 376, 1004, 1289
Tsp45I GTSAC 3 cut(s) 76, 370, 1864
TspDTI ATGAA 7 cut(s) 52, 382, 386, 426, 650, 1430, 1618
TspGWI ACGGA 2 cut(s) 443, 597
TspRI CASTG 1 cut(s) 1038
Van91I CCANNNNNTGG 1 cut(s) 659
VneI GTGCAC 1 cut(s) 1573
VpaK11BI GGWCC 1 cut(s) 584
XapI RAATTY 5 cut(s) 184, 617, 826, 1417, 1831
XceI RCATGY 3 cut(s) 366, 752, 1101
XcmI CCANNNNNNNNNTGG 3 cut(s) 1317, 1537, 1821
XmaJI CCTAGG 1 cut(s) 1462
XmiI GTMKAC 1 cut(s) 336
XmnI GAANNNNTTC 2 cut(s) 389, 1913
XspI CTAG 9 cut(s) 147, 564, 911, 930, 1463, 1620, 1688, 1787, 1841
ZrmI AGTACT 1 cut(s) 406
Zsp2I ATGCAT 1 cut(s) 1436
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.