Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr9

Orthogroup ID

OG0002188

Physical Location & Seq

Genomic Coordinates Forward (+)

5546047 .. 5549447

Gene Structure 3401 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom09g07360.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2262 bp

ATGCGTATTTATAAATATCCCGTTCTGTACCTTCCCTCCTCACACGCTCTCATCTCCTTCTCTCTTTTTTTTCTAGTCATTTCTCGTCTTGCTTCTTCAACCTATCTTAAATCCAGCACAGGACAAAAGGCCGCGAATTGTACTCAAGATCCACTCCAATCCAGGATTTTGGAAGTCCTTTGGATCAATATGGATACTGCGTATAAACCGAAGAACATCCTCCTTACTGGAGCTGCTGGCTTCATTGCTTCCCATGTTTGCAACCGGCTTATCCGGAACTACCCTGCCTACAAGATTGTGGTCCTTGACAAGCTTGATTACTGTTCAAATTTGAAGAACCTTAATCCTTCAAAGTCGTCTCCCAACTTTAAGTTTATCAAGGGAGACATAGGCAGTGCTGATCTTGTCAACTTCATCCTTTTGACCGAGAACATTGACACAATAATGCACTTTGCGGCCCAGACCCATGTCGACAACTCTTTTGGTAACAGCTTTGAGTTCACCAAAAACAACATCTACGGGACACATGTTCTTCTAGAAGCTTGCAAAGTCACTGGTCAAATCAAAAGATTCATTCATGTAAGCACAGACGAAGTCTATGGGGAGACAGATGAGGATGCTGTGGTGGGAAATCATGAGGCTTCTCAGCTTCTTCCGACAAACCCCTACTCTGCAACTAAAGCTGGAGCAGAGATGCTTGTTATGGCATATGGGCGTTCATATGGATTGCCTGTGATAACGACCAGAGGAAACAATGTTTATGGCCCCAACCAGTTCCCTGAAAAGATGATTCCAAAATTCATTCTCTTGGCTATACAAGTGAAGCCTCTTCCGATTCATGGTGATGGATCGAATGTCAGAAGTTATCTCTATTGTGAGGATGTAGCAGAGGCATTTGAAGTCATTCTCCATAGGGGTGAGGTAGGCCATGTGTACAACATTGGGACGAAGAAAGAGAGGAGGGTGGTTGATGTGGCCAAGGAAATTTGCCAACTATTTTCTCTGAACCCAGATACTTATGTAAAGTTTGTAGAGAACAGGCCCTTCAGGATCGATCAGAGATATTTCTTGGATGACCAGAAGCTGAAAAGCTTGGGATGGTCGGAAGGGACTTTGTGGGAGGAGGGTTTGAGGAAGACCATGGAATGGTATGTCAAGAATCCCCAATGGTGGGGAGATGTTTCTGGGGCACTGCTCCCTCATCCAAGAATGCTCATGGTTCCCGGAATTGAAAGACAATTTGATGGTTCCGATACTGGCGATTCTGCTTACCCTTTGTCAGCAAGTGATTCTAGTCCGAGCAAAATGGTTGTTCCAGCTCCAAGGAACATCCAATCTACTGAGAAGCCATCTCTGAAGTTTTTGATTTATGGTAAAACAGGGTGGATCGGAGGCCTTCTTGGGAAGTCTTGTGAGAAGCAAGGGATACCCTATGAGTATGGACGGGGGCGTCTTCAGGAACGGTCACAGCTCATGGCTGATATTCTGAGTGTTAAACCAACCCACGTTTTTAATGCTGCTGGAGTGACTGGCAGACCCAATGTGGATTGGTGTGAATCTCATAAGCCAGAAACAATTCGGACCAATGTTGTTGGTACATTAACCTTGGCTGATATCTGCAGAGATCACGGCCTCCTAATGGTGAATTATGCCACTGGTTGCATTTTCGAGTATGATGATGCCCATCCACCAAGATCGGAAATTGGGTTCAAGGAGGAAGACACACCGAATTTCGCGGGCTCATTTTATTCCAAAACCAAAGCCATGGTCCTTTCTCCAACCATCATATCTATTACTGTTGAAGAGCTTCTAAAGGAGTATGAGAATGTTTGCACTCTTAGAGTTCGAATGCCAATATCATCTGATCTCAGCAACCCACGCAACTTCATCACAAAGATCTCGCGCTACAATAAAGTGGTCGACATTCCAAACAGCATGACAATCTTGGACGAGCTTCTACCCATTTCAGTTGAGATGGCCAAGAGGAACTTGAGGGGCATATGGAACTTCACAAACCCCGGGGTGGTGAGTCACAACGAGATTCTGGAGATGTACAAGAACTACATTGACCCCAGTTTCAAGTGGACTAATTTTACTGTGGAAGAACAAGCCAAGGTTATCGTTGCAGCAAGAAGTAACAATGAGATGGATGCATCAAAGCTGAAGAAAGAGTTCCCGGAACTGCTCCCGATCAAGGAATCGTTGATTAAATACGTGTTTGAACCCAACAAGAAAACATTTGCAGGTGGAGCAGCAATTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005911 GO:0006139 GO:0006725 GO:0006793 GO:0006807 GO:0006810 GO:0008150 GO:0008152 GO:0009058 GO:0009225 GO:0009226 GO:0009506 GO:0009812 GO:0009813 GO:0009914 GO:0009966 GO:0009987 GO:0010253 GO:0010280 GO:0010315 GO:0010646 GO:0010817 GO:0010928 GO:0016829 GO:0016835 GO:0016836 GO:0018130 GO:0019438 GO:0023051 GO:0030054 GO:0030154 GO:0032502 GO:0033478 GO:0034641 GO:0034654 GO:0042127 GO:0042440 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046148 GO:0046483 GO:0048583 GO:0048869 GO:0050377 GO:0050789 GO:0050794 GO:0051179 GO:0051234 GO:0051552 GO:0051553 GO:0051554 GO:0051555 GO:0055044 GO:0055086 GO:0060918 GO:0065007 GO:0065008 GO:0071704 GO:1901135 GO:1901137 GO:1901360 GO:1901362 GO:1901576 GO:1901615 GO:1901617

KEGG Pathways

Metabolic & Signaling

ko00520 map00520

Pfam Domains

Protein Families

Epimerase GDP_Man_Dehyd

Protein Analysis

754

Amino Acids

84.72

Weight (kDa)

7.86

Isoelectric Point (pI)

36.24

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RmlD_sub_bind	PF04321	72 - 381	8e-12	RmlD substrate binding domain
Epimerase	PF01370	73 - 315	3.2e-55	NAD dependent epimerase/dehydratase family
Polysacc_synt_2	PF02719	73 - 344	8.5e-19	Polysaccharide biosynthesis protein
GDP_Man_Dehyd	PF16363	74 - 380	3e-61	GDP-mannose 4,6 dehydratase
3Beta_HSD	PF01073	75 - 298	6.4e-23	3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_4	PF07993	75 - 257	5.3e-19	Male sterility protein
RmlD_sub_bind	PF04321	453 - 588	1.4e-09	RmlD substrate binding domain
Epimerase	PF01370	456 - 590	1.1e-07	NAD dependent epimerase/dehydratase family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0002188)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G53500 AT3G14790 AT3G14790
fragaria_vesca	FvH4_2g36650 FvH4_2g36650 FvH4_6g38080 FvH4_6g38080 FvH4_6g38080 FvH4_6g38080 FvH4_6g38080
malus_domestica	MD09G1154300.v1.1 MD09G1154700.v1.1 MD15G1106300.v1.1 MD17G1140800.v1.1
prunus_persica	Prupe.1G458500_v2.0.a1 Prupe.3G170800_v2.0.a1
pyrus_communis	pycom09g07360 pycom15g09690 pycom17g13400
rosa_chinensis	RchiOBHm_Chr2g0151131 RchiOBHm_Chr6g0305141
rosa_laevigata	RLG00000010914 RLG00000020533
rosa_multiflora	Rmu_sc0000061.1_g000019 Rmu_sc0032179.1_g000002
rosa_roxburghii	Rroxscaffold_2G00096710 Rroxscaffold_7G00163120
rosa_rugosa	Rorug02G0425000 Rorug02G0425100 Rorug06G0338000 Rorug06G0338100 Rorug06G0338200 Rorug06G0338300
rosa_samantha	Rh2AG485600 Rh2BG497500 Rh2CG471400 Rh2DG508500 Rh6AG449700 Rh6BG451700 Rh6CG463100 Rh6DG450100
rosa_wichuraiana	Rw2G039770 Rw6G039160

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	12
AarI	CACCTGC	1 cut(s)	2234
AasI	GACNNNNNNGTC	1 cut(s)	1449
Acc36I	ACCTGC	1 cut(s)	2234
AccB7I	CCANNNNNTGG	1 cut(s)	1146
AccI	GTMKAC	2 cut(s)	471, 1920
AccII	CGCG	3 cut(s)	134, 1736, 1903
AccIII	TCCGGA	1 cut(s)	273
AciI	CCGC	3 cut(s)	132, 455, 1736
AclWI	GGATC	5 cut(s)	143, 191, 856, 1058, 1394
AcoI	YGGCCR	2 cut(s)	975, 1977
AcsI	RAATTY	4 cut(s)	328, 797, 984, 1729
AcuI	CTGAAG	4 cut(s)	1030, 1376, 1439, 2183
AcyI	GRCGYC	1 cut(s)	1450
AfaI	GTAC	5 cut(s)	29, 142, 935, 1597, 2054
AfiI	CCNNNNNNNGG	7 cut(s)	839, 1146, 1170, 1171, 1639, 2023, 2194
AflIII	ACRYGT	2 cut(s)	526, 2214
AjnI	CCWGG	1 cut(s)	161
AloI	GAACNNNNNNTCC	2 cut(s)	1691, 1723
Alw26I	GTCTC	3 cut(s)	363, 378, 599
AlwI	GGATC	5 cut(s)	143, 191, 856, 1058, 1394
AlwNI	CAGNNNCTG	1 cut(s)	1084
Ama87I	CYCGRG	1 cut(s)	2018
Aor13HI	TCCGGA	1 cut(s)	273
AoxI	GGCC	9 cut(s)	129, 456, 763, 925, 975, 1040, 1393, 1630, 1977
ApeKI	GCWGC	4 cut(s)	233, 1517, 2126, 2252
ApoI	RAATTY	4 cut(s)	328, 797, 984, 1729
ArsI	GACNNNNNNTTYG	4 cut(s)	464, 496, 1752, 1784
Asp700I	GAANNNNTTC	4 cut(s)	903, 1575, 1806, 2171
AspLEI	GCGC	1 cut(s)	1905
AspS9I	GGNCC	6 cut(s)	301, 457, 764, 1041, 1581, 1768
AsuC2I	CCSGG	4 cut(s)	1224, 2019, 2020, 2177
AsuHPI	GGTGA	5 cut(s)	493, 854, 929, 1654, 2038
AsuII	TTCGAA	1 cut(s)	1846
AvaI	CYCGRG	1 cut(s)	2018
AvaII	GGWCC	3 cut(s)	301, 1581, 1768
BaeGI	GKGCMC	1 cut(s)	1192
BaeI	ACNNNNGTAYC	2 cut(s)	1005, 1038
BalI	TGGCCA	2 cut(s)	977, 1979
BanII	GRGCYC	1 cut(s)	1742
BbsI	GAAGAC	3 cut(s)	1142, 1445, 1725
BbvI	GCAGC	3 cut(s)	220, 1504, 2138
BccI	CCATC	8 cut(s)	839, 1092, 1238, 1357, 1692, 1790, 1969, 2140
BceAI	ACGGC	1 cut(s)	1645
BciT130I	CCWGG	1 cut(s)	163
BciVI	GTATCC	2 cut(s)	187, 1419
BcnI	CCSGG	4 cut(s)	1224, 2019, 2020, 2177
BcoDI	GTCTC	3 cut(s)	363, 378, 599
BfaI	CTAG	3 cut(s)	74, 536, 1293
BfmI	CTRYAG	1 cut(s)	1618
BfuAI	ACCTGC	1 cut(s)	2234
BfuI	GTATCC	2 cut(s)	187, 1419
BglII	AGATCT	1 cut(s)	1896
BisI	GCNGC	6 cut(s)	132, 234, 456, 1518, 2127, 2253
BlsI	GCNGC	6 cut(s)	133, 235, 457, 1519, 2128, 2254
Bme1390I	CCNGG	5 cut(s)	163, 1224, 2019, 2020, 2177
Bme18I	GGWCC	3 cut(s)	301, 1581, 1768
BmeT110I	CYCGRG	1 cut(s)	2018
BmgT120I	GGNCC	6 cut(s)	301, 457, 764, 1041, 1581, 1768
BmiI	GGNNCC	3 cut(s)	766, 1221, 1249
BmrFI	CCNGG	5 cut(s)	163, 1224, 2019, 2020, 2177
BmrI	ACTGGG	1 cut(s)	2067
BmsI	GCATC	5 cut(s)	607, 684, 1669, 2140, 2162
BmuI	ACTGGG	1 cut(s)	2067
BoxI	GACNNNNGTC	1 cut(s)	467
BpiI	GAAGAC	3 cut(s)	1142, 1445, 1725
BpmI	CTGGAG	4 cut(s)	249, 705, 1542, 2066
Bpu14I	TTCGAA	1 cut(s)	1846
BpuEI	CTTGAG	2 cut(s)	129, 2011
BpuMI	CCSGG	4 cut(s)	1224, 2019, 2020, 2177
Bsa29I	ATCGAT	1 cut(s)	1053
BsaAI	YACGTR	1 cut(s)	2215
BsaBI	GATNNNNATC	1 cut(s)	1683
BsaHI	GRCGYC	1 cut(s)	1450
BsaJI	CCNNGG	9 cut(s)	978, 1140, 1322, 1605, 1764, 2017, 2018, 2019, 2112
BsaWI	WCCGGW	1 cut(s)	273
BsaXI	ACNNNNNCTCC	2 cut(s)	20, 50
Bsc4I	CCNNNNNNNGG	7 cut(s)	839, 1146, 1170, 1171, 1639, 2023, 2194
Bse118I	RCCGGY	1 cut(s)	264
Bse1I	ACTGG	7 cut(s)	232, 559, 772, 1261, 1534, 1660, 2073
Bse3DI	GCAATG	1 cut(s)	243
Bse8I	GATNNNNATC	1 cut(s)	1683
BseAI	TCCGGA	1 cut(s)	273
BseBI	CCWGG	1 cut(s)	163
BseCI	ATCGAT	1 cut(s)	1053
BseDI	CCNNGG	9 cut(s)	978, 1140, 1322, 1605, 1764, 2017, 2018, 2019, 2112
BseJI	GATNNNNATC	1 cut(s)	1683
BseLI	CCNNNNNNNGG	7 cut(s)	839, 1146, 1170, 1171, 1639, 2023, 2194
BseMI	GCAATG	1 cut(s)	243
BseMII	CTCAG	4 cut(s)	659, 1332, 1478, 1882
BseNI	ACTGG	7 cut(s)	232, 559, 772, 1261, 1534, 1660, 2073
BseRI	GAGGAG	3 cut(s)	28, 973, 1136
BseSI	GKGCMC	1 cut(s)	1192
BseXI	GCAGC	3 cut(s)	220, 1504, 2138
Bsh1236I	CGCG	3 cut(s)	134, 1736, 1903
BshFI	GGCC	9 cut(s)	131, 458, 765, 927, 977, 1042, 1395, 1632, 1979
BshVI	ATCGAT	1 cut(s)	1053
BsiHKCI	CYCGRG	1 cut(s)	2018
BsiSI	CCGG	5 cut(s)	265, 274, 1224, 2019, 2177
BslFI	GGGAC	3 cut(s)	535, 958, 1123
BslI	CCNNNNNNNGG	7 cut(s)	839, 1146, 1170, 1171, 1639, 2023, 2194
BsmAI	GTCTC	3 cut(s)	363, 378, 599
BsmBI	CGTCTC	1 cut(s)	363
BsmFI	GGGAC	3 cut(s)	535, 958, 1123
BsmI	GAATGC	2 cut(s)	1215, 1854
BsnI	GGCC	9 cut(s)	131, 458, 765, 927, 977, 1042, 1395, 1632, 1979
BsoBI	CYCGRG	1 cut(s)	2018
Bsp119I	TTCGAA	1 cut(s)	1846
Bsp1286I	GDGCHC	2 cut(s)	1192, 1742
Bsp13I	TCCGGA	1 cut(s)	273
Bsp1407I	TGTACA	2 cut(s)	933, 2052
Bsp19I	CCATGG	2 cut(s)	1140, 1764
BspACI	CCGC	3 cut(s)	132, 455, 1736
BspANI	GGCC	9 cut(s)	131, 458, 765, 927, 977, 1042, 1395, 1632, 1979
BspCNI	CTCAG	4 cut(s)	658, 1333, 1479, 1881
BspDI	ATCGAT	1 cut(s)	1053
BspEI	TCCGGA	1 cut(s)	273
BspFNI	CGCG	3 cut(s)	134, 1736, 1903
BspHI	TCATGA	1 cut(s)	634
BspLI	GGNNCC	3 cut(s)	766, 1221, 1249
BspMAI	CTGCAG	1 cut(s)	1622
BspMI	ACCTGC	1 cut(s)	2234
BspPI	GGATC	5 cut(s)	143, 191, 856, 1058, 1394
BspQI	GCTCTTC	1 cut(s)	1797
BspT104I	TTCGAA	1 cut(s)	1846
BsrDI	GCAATG	1 cut(s)	243
BsrFI	RCCGGY	1 cut(s)	264
BsrGI	TGTACA	2 cut(s)	933, 2052
BsrI	ACTGG	7 cut(s)	232, 559, 772, 1261, 1534, 1660, 2073
BssAI	RCCGGY	1 cut(s)	264
BssECI	CCNNGG	9 cut(s)	978, 1140, 1322, 1605, 1764, 2017, 2018, 2019, 2112
BssNI	GRCGYC	1 cut(s)	1450
BssT1I	CCWWGG	6 cut(s)	978, 1140, 1322, 1605, 1764, 2112
Bst2UI	CCWGG	1 cut(s)	163
Bst4CI	ACNGT	4 cut(s)	323, 1464, 1798, 2098
Bst6I	CTCTTC	2 cut(s)	834, 1797
BstACI	GRCGYC	1 cut(s)	1450
BstAUI	TGTACA	2 cut(s)	933, 2052
BstBAI	YACGTR	1 cut(s)	2215
BstBI	TTCGAA	1 cut(s)	1846
BstC8I	GCNNGC	3 cut(s)	238, 544, 1738
BstDEI	CTNAG	5 cut(s)	645, 1341, 1487, 1838, 1868
BstDSI	CCRYGG	2 cut(s)	1140, 1764
BstFNI	CGCG	3 cut(s)	134, 1736, 1903
BstHHI	GCGC	1 cut(s)	1905
BstMAI	GTCTC	3 cut(s)	363, 378, 599
BstMWI	GCNNNNNNNGC	3 cut(s)	680, 1878, 2249
BstNI	CCWGG	1 cut(s)	163
BstNSI	RCATGY	1 cut(s)	530
BstPAI	GACNNNNGTC	1 cut(s)	467
BstSCI	CCNGG	5 cut(s)	161, 1222, 2017, 2018, 2175
BstSFI	CTRYAG	1 cut(s)	1618
BstSLI	GKGCMC	1 cut(s)	1192
BstUI	CGCG	3 cut(s)	134, 1736, 1903
BstV1I	GCAGC	3 cut(s)	220, 1504, 2138
BstV2I	GAAGAC	3 cut(s)	1142, 1445, 1725
BstX2I	RGATCY	2 cut(s)	148, 1896
BstXI	CCANNNNNNTGG	2 cut(s)	169, 1765
BstYI	RGATCY	2 cut(s)	148, 1896
Bsu15I	ATCGAT	1 cut(s)	1053
BsuI	GTATCC	2 cut(s)	187, 1419
BsuRI	GGCC	9 cut(s)	131, 458, 765, 927, 977, 1042, 1395, 1632, 1979
BsuTUI	ATCGAT	1 cut(s)	1053
BtgI	CCRYGG	2 cut(s)	1140, 1764
BtsI	GCAGTG	2 cut(s)	400, 1190
BtsIMutI	CAGTG	4 cut(s)	400, 552, 1190, 1653
BveI	ACCTGC	1 cut(s)	2234
Cac8I	GCNNGC	3 cut(s)	238, 544, 1738
CaiI	CAGNNNCTG	1 cut(s)	1084
CciI	TCATGA	1 cut(s)	634
CfoI	GCGC	1 cut(s)	1905
Cfr10I	RCCGGY	1 cut(s)	264
Cfr13I	GGNCC	6 cut(s)	301, 457, 764, 1041, 1581, 1768
Cfr9I	CCCGGG	1 cut(s)	2018
ClaI	ATCGAT	1 cut(s)	1053
CseI	GACGC	1 cut(s)	1439
Csp6I	GTAC	5 cut(s)	28, 141, 934, 1596, 2053
CviQI	GTAC	5 cut(s)	28, 141, 934, 1596, 2053
DdeI	CTNAG	5 cut(s)	645, 1341, 1487, 1838, 1868
DrdI	GACNNNNNNGTC	1 cut(s)	1449
DseDI	GACNNNNNNGTC	1 cut(s)	1449
EaeI	YGGCCR	2 cut(s)	975, 1977
Eam1104I	CTCTTC	2 cut(s)	834, 1797
EarI	CTCTTC	2 cut(s)	834, 1797
Eco130I	CCWWGG	6 cut(s)	978, 1140, 1322, 1605, 1764, 2112
Eco147I	AGGCCT	1 cut(s)	1395
Eco24I	GRGCYC	1 cut(s)	1742
Eco32I	GATATC	1 cut(s)	1615
Eco47I	GGWCC	3 cut(s)	301, 1581, 1768
Eco57I	CTGAAG	4 cut(s)	1030, 1376, 1439, 2183
Eco88I	CYCGRG	1 cut(s)	2018
EcoO109I	RGGNCCY	1 cut(s)	1041
EcoRII	CCWGG	1 cut(s)	161
EcoRV	GATATC	1 cut(s)	1615
EcoT14I	CCWWGG	6 cut(s)	978, 1140, 1322, 1605, 1764, 2112
EcoT22I	ATGCAT	1 cut(s)	2155
EcoT38I	GRGCYC	1 cut(s)	1742
ErhI	CCWWGG	6 cut(s)	978, 1140, 1322, 1605, 1764, 2112
Esp3I	CGTCTC	1 cut(s)	363
FalI	AAGNNNNNCTT	2 cut(s)	1973, 2005
FaqI	GGGAC	3 cut(s)	535, 958, 1123
FauI	CCCGC	1 cut(s)	1729
FauNDI	CATATG	3 cut(s)	709, 721, 2000
FblI	GTMKAC	2 cut(s)	471, 1920
Fnu4HI	GCNGC	6 cut(s)	132, 234, 456, 1518, 2127, 2253
FriOI	GRGCYC	1 cut(s)	1742
Fsp4HI	GCNGC	6 cut(s)	132, 234, 456, 1518, 2127, 2253
FspBI	CTAG	3 cut(s)	74, 536, 1293
GlaI	GCGC	1 cut(s)	1904
GluI	GCNGC	6 cut(s)	132, 234, 456, 1518, 2127, 2253
GsuI	CTGGAG	4 cut(s)	249, 705, 1542, 2066
HaeIII	GGCC	9 cut(s)	131, 458, 765, 927, 977, 1042, 1395, 1632, 1979
HapII	CCGG	5 cut(s)	265, 274, 1224, 2019, 2177
HgaI	GACGC	1 cut(s)	1439
HhaI	GCGC	1 cut(s)	1905
Hin1I	GRCGYC	1 cut(s)	1450
Hin6I	GCGC	1 cut(s)	1903
HinP1I	GCGC	1 cut(s)	1903
HincII	GTYRAC	3 cut(s)	409, 472, 1921
HindII	GTYRAC	3 cut(s)	409, 472, 1921
HindIII	AAGCTT	3 cut(s)	311, 540, 1090
HpaII	CCGG	5 cut(s)	265, 274, 1224, 2019, 2177
HphI	GGTGA	5 cut(s)	493, 854, 929, 1654, 2038
Hpy166II	GTNNAC	6 cut(s)	409, 472, 501, 934, 1921, 2085
Hpy188III	TCNNGA	9 cut(s)	146, 274, 536, 635, 1048, 1156, 1457, 2045, 2188
Hpy8I	GTNNAC	6 cut(s)	409, 472, 501, 934, 1921, 2085
HpyAV	CCTTC	6 cut(s)	41, 67, 357, 1054, 1100, 1406
HpyCH4III	ACNGT	4 cut(s)	323, 1464, 1798, 2098
HpyCH4IV	ACGT	2 cut(s)	1506, 2214
HpyF10VI	GCNNNNNNNGC	3 cut(s)	680, 1878, 2249
HpyF3I	CTNAG	5 cut(s)	645, 1341, 1487, 1838, 1868
HpySE526I	ACGT	2 cut(s)	1506, 2214
Hsp92I	GRCGYC	1 cut(s)	1450
HspAI	GCGC	1 cut(s)	1903
Kpn2I	TCCGGA	1 cut(s)	273
LguI	GCTCTTC	1 cut(s)	1797
LmnI	GCTCC	6 cut(s)	230, 686, 1200, 1324, 2190, 2249
Lsp1109I	GCAGC	3 cut(s)	220, 1504, 2138
LweI	GCATC	5 cut(s)	607, 684, 1669, 2140, 2162
MaeI	CTAG	3 cut(s)	74, 536, 1293
MaeII	ACGT	2 cut(s)	1506, 2214
MaeIII	GTNAC	6 cut(s)	485, 550, 1464, 1525, 2030, 2135
MflI	RGATCY	2 cut(s)	148, 1896
MhlI	GDGCHC	2 cut(s)	1192, 1742
MlsI	TGGCCA	2 cut(s)	977, 1979
MluNI	TGGCCA	2 cut(s)	977, 1979
MlyI	GAGTC	1 cut(s)	2038
MmeI	TCCRAC	3 cut(s)	680, 1083, 1802
Mox20I	TGGCCA	2 cut(s)	977, 1979
Mph1103I	ATGCAT	1 cut(s)	2155
MroI	TCCGGA	1 cut(s)	273
MroXI	GAANNNNTTC	4 cut(s)	903, 1575, 1806, 2171
MscI	TGGCCA	2 cut(s)	977, 1979
MseI	TTAA	8 cut(s)	108, 342, 369, 1494, 1512, 1601, 2208, 2260
MslI	CAYNNNNRTG	3 cut(s)	443, 843, 915
Msp20I	TGGCCA	2 cut(s)	977, 1979
MspI	CCGG	5 cut(s)	265, 274, 1224, 2019, 2177
MspR9I	CCNGG	5 cut(s)	163, 1224, 2019, 2020, 2177
Mva1269I	GAATGC	2 cut(s)	1215, 1854
MvaI	CCWGG	1 cut(s)	163
MvnI	CGCG	3 cut(s)	134, 1736, 1903
MwoI	GCNNNNNNNGC	3 cut(s)	680, 1878, 2249
NciI	CCSGG	4 cut(s)	1224, 2019, 2020, 2177
NcoI	CCATGG	2 cut(s)	1140, 1764
NdeI	CATATG	3 cut(s)	709, 721, 2000
NlaIV	GGNNCC	3 cut(s)	766, 1221, 1249
NmuCI	GTSAC	4 cut(s)	550, 1464, 1525, 2030
NsiI	ATGCAT	1 cut(s)	2155
NspI	RCATGY	1 cut(s)	530
NspV	TTCGAA	1 cut(s)	1846
PagI	TCATGA	1 cut(s)	634
PaqCI	CACCTGC	1 cut(s)	2234
PceI	AGGCCT	1 cut(s)	1395
PciI	ACATGT	1 cut(s)	526
PciSI	GCTCTTC	1 cut(s)	1797
PctI	GAATGC	2 cut(s)	1215, 1854
PdmI	GAANNNNTTC	4 cut(s)	903, 1575, 1806, 2171
PfeI	GAWTC	9 cut(s)	570, 790, 835, 1159, 1262, 1289, 1556, 2041, 2198
PflFI	GACNNNGTC	1 cut(s)	593
PflMI	CCANNNNNTGG	1 cut(s)	1146
PfoI	TCCNGGA	3 cut(s)	161, 1222, 2175
PkrI	GCNGC	6 cut(s)	133, 235, 457, 1519, 2128, 2254
PleI	GAGTC	1 cut(s)	2037
PpsI	GAGTC	1 cut(s)	2037
Ppu21I	YACGTR	1 cut(s)	2215
PscI	ACATGT	1 cut(s)	526
PshAI	GACNNNNGTC	1 cut(s)	467
PsiI	TTATAA	1 cut(s)	12
Psp6I	CCWGG	1 cut(s)	161
PspGI	CCWGG	1 cut(s)	161
PspN4I	GGNNCC	3 cut(s)	766, 1221, 1249
PspPI	GGNCC	6 cut(s)	301, 457, 764, 1041, 1581, 1768
PstI	CTGCAG	1 cut(s)	1622
PstNI	CAGNNNCTG	1 cut(s)	1084
PsuI	RGATCY	2 cut(s)	148, 1896
PsyI	GACNNNGTC	1 cut(s)	593
RsaI	GTAC	5 cut(s)	29, 142, 935, 1597, 2054
RsaNI	GTAC	5 cut(s)	28, 141, 934, 1596, 2053
RseI	CAYNNNNRTG	3 cut(s)	443, 843, 915
SalI	GTCGAC	2 cut(s)	470, 1919
SapI	GCTCTTC	1 cut(s)	1797
SaqAI	TTAA	8 cut(s)	108, 342, 369, 1494, 1512, 1601, 2208, 2260
SatI	GCNGC	6 cut(s)	132, 234, 456, 1518, 2127, 2253
Sau96I	GGNCC	6 cut(s)	301, 457, 764, 1041, 1581, 1768
SchI	GAGTC	1 cut(s)	2038
ScrFI	CCNGG	5 cut(s)	163, 1224, 2019, 2020, 2177
SduI	GDGCHC	2 cut(s)	1192, 1742
SfaNI	GCATC	5 cut(s)	607, 684, 1669, 2140, 2162
SfcI	CTRYAG	1 cut(s)	1618
SfuI	TTCGAA	1 cut(s)	1846
SinI	GGWCC	3 cut(s)	301, 1581, 1768
SmaI	CCCGGG	1 cut(s)	2020
SmiMI	CAYNNNNRTG	3 cut(s)	443, 843, 915
SmlI	CTYRAG	2 cut(s)	144, 1990
SmoI	CTYRAG	2 cut(s)	144, 1990
SseBI	AGGCCT	1 cut(s)	1395
SsiI	CCGC	3 cut(s)	132, 455, 1736
SspMI	CTAG	3 cut(s)	74, 536, 1293
StuI	AGGCCT	1 cut(s)	1395
StyD4I	CCNGG	5 cut(s)	161, 1222, 2017, 2018, 2175
StyI	CCWWGG	6 cut(s)	978, 1140, 1322, 1605, 1764, 2112
TaaI	ACNGT	4 cut(s)	323, 1464, 1798, 2098
TaiI	ACGT	2 cut(s)	1509, 2217
TaqI	TCGA	6 cut(s)	471, 851, 1053, 1668, 1846, 1920
TaqII	GACCGA	1 cut(s)	440
TatI	WGTACW	3 cut(s)	140, 933, 2052
TauI	GCSGC	2 cut(s)	134, 458
TfiI	GAWTC	9 cut(s)	570, 790, 835, 1159, 1262, 1289, 1556, 2041, 2198
Tru1I	TTAA	8 cut(s)	108, 342, 369, 1494, 1512, 1601, 2208, 2260
Tru9I	TTAA	8 cut(s)	108, 342, 369, 1494, 1512, 1601, 2208, 2260
TscAI	CASTG	4 cut(s)	400, 559, 1197, 1660
TseFI	GTSAC	4 cut(s)	550, 1464, 1525, 2030
TseI	GCWGC	4 cut(s)	233, 1517, 2126, 2252
Tsp45I	GTSAC	4 cut(s)	550, 1464, 1525, 2030
TspDTI	ATGAA	8 cut(s)	232, 403, 562, 566, 708, 790, 827, 1876
TspMI	CCCGGG	1 cut(s)	2018
TspRI	CASTG	4 cut(s)	400, 559, 1197, 1660
Tth111I	GACNNNGTC	1 cut(s)	593
Van91I	CCANNNNNTGG	1 cut(s)	1146
VpaK11BI	GGWCC	3 cut(s)	301, 1581, 1768
XapI	RAATTY	4 cut(s)	328, 797, 984, 1729
XbaI	TCTAGA	1 cut(s)	535
XceI	RCATGY	1 cut(s)	530
XmaI	CCCGGG	1 cut(s)	2018
XmiI	GTMKAC	2 cut(s)	471, 1920
XmnI	GAANNNNTTC	4 cut(s)	903, 1575, 1806, 2171
XspI	CTAG	3 cut(s)	74, 536, 1293
Zsp2I	ATGCAT	1 cut(s)	2155

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.