Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0002188

Physical Location & Seq

Genomic Coordinates Forward (+)

12290226 .. 12292802

Gene Structure 2577 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1154700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1320 bp

ATGGATACTGCGTATAAACCGAAGAACATCCTCCTTACTGGAGCTGCTGGCTTCATTGCTTCCCATGTTTGCAACCGGCTTATCCGGAACTACCCTGCCTACAAGATTGTGGTCCTTGACAAGCTTGATTACTGTTCAAATTTGAAGAACCTTAATCCTTCAAAGTCGTCCCCCAAGTTTAAGTTTATCAAAGGAGACATAGGCAGTGCTGATCTTGTCAACTTCATCCTTTTGACCGAGAACATTGACACAATAATGCACTTTGCAGCCCAGACCCATGTCGACAACTCTTTTGGTAACAGCTTTGAGTTCACCAAAAACAACATCTACGGGACACATGTTCTTCTAGAAGCTTGCAAAGTCACTGGTCAAATCAAAAGATTCATTCATGTAAGCACAGATGAAGTCTATGGGGAGACAGATGAGGATGCTGTGGTGGGAAATCATGAGGCTTCTCAGCTTCTTCCGACAAACCCCTACTCTGCAACTAAAGCTGGAGCAGAGATGCTTGTCATGGCATATGGGCGTTCATATGGATTGCCTGTGATAACGACCAGAGGAAACAATGTTTATGGCCCCAACCAGTTCCCTGAAAAGATGATTCCAAAATTCATTCTCTTGGCTATGCAAGGGAAGCCTCTTCCGATTCATGGTGATGGATCCAATGTCAGAAGTTATCTCTATTGTGAGGATGTAGCAGAGGCATTTGAAGTCATTCTCCATAGGGGTGAGGTAGGCCATGTGTACAACATTGGGACGAAGAAAGAGAGGAGGGTGGTCGATGTGGCCAGGGAAATTTGCCAACTATTTTCTCTGAACCCAGATACATATGTAAAGTTTGTAGAGAACAGACCCTTCAATGATCAGAGATATTTCTTGGATGACCAGAAGCTGAAAAGCTTGGGATGGTCGGAAAGGACTTTGTGGGAGGAGGGTTTGAGGAAGACCATGGAATGGTATGTCAAGAATCCCCAATGGTGGGGAGATGTTTCTGGGGCACTGCTCCCTCATCCAAAAATGCTCATGGTTCCCGGAATTGAAAGACAATTTGATGGTTCCGATACTGGCGATTCTGCTTACCCTTTGTCAGCAAGTGATTCTAGTCAGAGCAAAATGGTTGTTCCAGCTCCAAGGAACATCCAATCTACTGAGAAGCCATCTCTGAAGTTTTTGATTTATGGTAAAACAGGGTGGATCGGAGGCCTTCTTGGGAAGATTTGTGAGAAGCAAGGGTTACCCTATGAGTATGGACGAGGGCGTCTTCAGGAACGGTCACAGCTCATGGCTGATATTCTGAGTACCCAATGTGGATTGGTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005911 GO:0006139 GO:0006725 GO:0006793 GO:0006807 GO:0006810 GO:0008150 GO:0008152 GO:0009058 GO:0009225 GO:0009226 GO:0009506 GO:0009812 GO:0009813 GO:0009914 GO:0009966 GO:0009987 GO:0010253 GO:0010280 GO:0010315 GO:0010646 GO:0010817 GO:0010928 GO:0016829 GO:0016835 GO:0016836 GO:0018130 GO:0019438 GO:0023051 GO:0030054 GO:0030154 GO:0032502 GO:0033478 GO:0034641 GO:0034654 GO:0042127 GO:0042440 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046148 GO:0046483 GO:0048583 GO:0048869 GO:0050377 GO:0050789 GO:0050794 GO:0051179 GO:0051234 GO:0051552 GO:0051553 GO:0051554 GO:0051555 GO:0055044 GO:0055086 GO:0060918 GO:0065007 GO:0065008 GO:0071704 GO:1901135 GO:1901137 GO:1901360 GO:1901362 GO:1901576 GO:1901615 GO:1901617

KEGG Pathways

Metabolic & Signaling

ko00520 map00520

Pfam Domains

Protein Families

Epimerase GDP_Man_Dehyd

Protein Analysis

440

Amino Acids

49.25

Weight (kDa)

7.6

Isoelectric Point (pI)

35.14

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RmlD_sub_bind	PF04321	9 - 318	2.8e-13	RmlD substrate binding domain
Epimerase	PF01370	10 - 252	8.1e-57	NAD dependent epimerase/dehydratase family
Polysacc_synt_2	PF02719	10 - 281	7.3e-21	Polysaccharide biosynthesis protein
GDP_Man_Dehyd	PF16363	11 - 316	2.5e-64	GDP-mannose 4,6 dehydratase
3Beta_HSD	PF01073	12 - 235	4.4e-24	3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_4	PF07993	12 - 194	4.5e-20	Male sterility protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0002188)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G53500 AT3G14790 AT3G14790
fragaria_vesca	FvH4_2g36650 FvH4_2g36650 FvH4_6g38080 FvH4_6g38080 FvH4_6g38080 FvH4_6g38080 FvH4_6g38080
malus_domestica	MD09G1154300.v1.1 MD09G1154700.v1.1 MD15G1106300.v1.1 MD17G1140800.v1.1
prunus_persica	Prupe.1G458500_v2.0.a1 Prupe.3G170800_v2.0.a1
pyrus_communis	pycom09g07360 pycom15g09690 pycom17g13400
rosa_chinensis	RchiOBHm_Chr2g0151131 RchiOBHm_Chr6g0305141
rosa_laevigata	RLG00000010914 RLG00000020533
rosa_multiflora	Rmu_sc0000061.1_g000019 Rmu_sc0032179.1_g000002
rosa_roxburghii	Rroxscaffold_2G00096710 Rroxscaffold_7G00163120
rosa_rugosa	Rorug02G0425000 Rorug02G0425100 Rorug06G0338000 Rorug06G0338100 Rorug06G0338200 Rorug06G0338300
rosa_samantha	Rh2AG485600 Rh2BG497500 Rh2CG471400 Rh2DG508500 Rh6AG449700 Rh6BG451700 Rh6CG463100 Rh6DG450100
rosa_wichuraiana	Rw2G039770 Rw6G039160

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1257
AccB7I	CCANNNNNTGG	1 cut(s)	954
AccI	GTMKAC	1 cut(s)	282
AccIII	TCCGGA	1 cut(s)	84
AclWI	GGATC	3 cut(s)	654, 667, 1202
AcoI	YGGCCR	1 cut(s)	786
AcsI	RAATTY	3 cut(s)	139, 608, 795
AcuI	CTGAAG	2 cut(s)	1184, 1247
AcyI	GRCGYC	1 cut(s)	1258
AfaI	GTAC	2 cut(s)	746, 1300
AfiI	CCNNNNNNNGG	4 cut(s)	650, 954, 978, 979
AflIII	ACRYGT	1 cut(s)	337
AgsI	TTSAA	6 cut(s)	138, 145, 162, 710, 859, 1040
AjnI	CCWGG	1 cut(s)	788
Alw26I	GTCTC	2 cut(s)	189, 410
AlwI	GGATC	3 cut(s)	654, 667, 1202
AlwNI	CAGNNNCTG	1 cut(s)	892
Aor13HI	TCCGGA	1 cut(s)	84
AoxI	GGCC	4 cut(s)	574, 736, 786, 1201
ApeKI	GCWGC	2 cut(s)	44, 266
ApoI	RAATTY	3 cut(s)	139, 608, 795
ArsI	GACNNNNNNTTYG	2 cut(s)	275, 307
Asp700I	GAANNNNTTC	1 cut(s)	714
AspS9I	GGNCC	2 cut(s)	112, 575
AsuC2I	CCSGG	1 cut(s)	1032
AsuHPI	GGTGA	3 cut(s)	304, 665, 740
AvaII	GGWCC	1 cut(s)	112
BaeGI	GKGCMC	1 cut(s)	1000
BaeI	ACNNNNGTAYC	2 cut(s)	816, 849
BalI	TGGCCA	1 cut(s)	788
BamHI	GGATCC	1 cut(s)	659
BbsI	GAAGAC	2 cut(s)	950, 1253
BbvI	GCAGC	2 cut(s)	31, 278
BccI	CCATC	4 cut(s)	650, 900, 1046, 1165
BciT130I	CCWGG	1 cut(s)	790
BclI	TGATCA	1 cut(s)	862
BcnI	CCSGG	1 cut(s)	1032
BcoDI	GTCTC	2 cut(s)	189, 410
BfaI	CTAG	2 cut(s)	347, 1101
BisI	GCNGC	2 cut(s)	45, 267
BlsI	GCNGC	2 cut(s)	46, 268
Bme1390I	CCNGG	2 cut(s)	790, 1032
Bme18I	GGWCC	1 cut(s)	112
BmgT120I	GGNCC	2 cut(s)	112, 575
BmiI	GGNNCC	4 cut(s)	577, 661, 1029, 1057
BmrFI	CCNGG	2 cut(s)	790, 1032
BmsI	GCATC	2 cut(s)	418, 495
BoxI	GACNNNNGTC	1 cut(s)	278
BpiI	GAAGAC	2 cut(s)	950, 1253
BpmI	CTGGAG	2 cut(s)	60, 516
BpuMI	CCSGG	1 cut(s)	1032
BsaHI	GRCGYC	1 cut(s)	1258
BsaJI	CCNNGG	3 cut(s)	789, 948, 1130
BsaWI	WCCGGW	1 cut(s)	84
Bsc4I	CCNNNNNNNGG	4 cut(s)	650, 954, 978, 979
Bse118I	RCCGGY	1 cut(s)	75
Bse1I	ACTGG	4 cut(s)	43, 370, 583, 1069
Bse3DI	GCAATG	1 cut(s)	54
BseAI	TCCGGA	1 cut(s)	84
BseBI	CCWGG	1 cut(s)	790
BseDI	CCNNGG	3 cut(s)	789, 948, 1130
BseGI	GGATG	8 cut(s)	27, 225, 433, 697, 886, 911, 1009, 1137
BseLI	CCNNNNNNNGG	4 cut(s)	650, 954, 978, 979
BseMI	GCAATG	1 cut(s)	54
BseMII	CTCAG	3 cut(s)	470, 1140, 1286
BseNI	ACTGG	4 cut(s)	43, 370, 583, 1069
BseRI	GAGGAG	2 cut(s)	784, 944
BseSI	GKGCMC	1 cut(s)	1000
BseXI	GCAGC	2 cut(s)	31, 278
BshFI	GGCC	4 cut(s)	576, 738, 788, 1203
BsiSI	CCGG	3 cut(s)	76, 85, 1032
BslFI	GGGAC	3 cut(s)	154, 346, 769
BslI	CCNNNNNNNGG	4 cut(s)	650, 954, 978, 979
BsmAI	GTCTC	2 cut(s)	189, 410
BsmFI	GGGAC	3 cut(s)	154, 346, 769
BsnI	GGCC	4 cut(s)	576, 738, 788, 1203
Bsp1286I	GDGCHC	1 cut(s)	1000
Bsp13I	TCCGGA	1 cut(s)	84
Bsp1407I	TGTACA	1 cut(s)	744
Bsp143I	GATC	4 cut(s)	211, 659, 862, 1194
Bsp19I	CCATGG	1 cut(s)	948
BspANI	GGCC	4 cut(s)	576, 738, 788, 1203
BspCNI	CTCAG	3 cut(s)	469, 1141, 1287
BspEI	TCCGGA	1 cut(s)	84
BspHI	TCATGA	1 cut(s)	445
BspLI	GGNNCC	4 cut(s)	577, 661, 1029, 1057
BspPI	GGATC	3 cut(s)	654, 667, 1202
BsrDI	GCAATG	1 cut(s)	54
BsrFI	RCCGGY	1 cut(s)	75
BsrGI	TGTACA	1 cut(s)	744
BsrI	ACTGG	4 cut(s)	43, 370, 583, 1069
BssAI	RCCGGY	1 cut(s)	75
BssECI	CCNNGG	3 cut(s)	789, 948, 1130
BssMI	GATC	4 cut(s)	211, 659, 862, 1194
BssNI	GRCGYC	1 cut(s)	1258
BssT1I	CCWWGG	2 cut(s)	948, 1130
Bst2UI	CCWGG	1 cut(s)	790
Bst4CI	ACNGT	2 cut(s)	134, 1272
Bst6I	CTCTTC	1 cut(s)	645
BstACI	GRCGYC	1 cut(s)	1258
BstAUI	TGTACA	1 cut(s)	744
BstC8I	GCNNGC	2 cut(s)	49, 355
BstDEI	CTNAG	3 cut(s)	456, 1149, 1295
BstDSI	CCRYGG	1 cut(s)	948
BstEII	GGTNACC	1 cut(s)	1233
BstF5I	GGATG	8 cut(s)	27, 225, 433, 697, 886, 911, 1009, 1137
BstKTI	GATC	4 cut(s)	214, 662, 865, 1197
BstMAI	GTCTC	2 cut(s)	189, 410
BstMBI	GATC	4 cut(s)	211, 659, 862, 1194
BstMWI	GCNNNNNNNGC	2 cut(s)	491, 634
BstNI	CCWGG	1 cut(s)	790
BstNSI	RCATGY	1 cut(s)	341
BstPAI	GACNNNNGTC	1 cut(s)	278
BstPI	GGTNACC	1 cut(s)	1233
BstSCI	CCNGG	2 cut(s)	788, 1030
BstSLI	GKGCMC	1 cut(s)	1000
BstV1I	GCAGC	2 cut(s)	31, 278
BstV2I	GAAGAC	2 cut(s)	950, 1253
BstX2I	RGATCY	1 cut(s)	659
BstYI	RGATCY	1 cut(s)	659
BsuRI	GGCC	4 cut(s)	576, 738, 788, 1203
BtgI	CCRYGG	1 cut(s)	948
BtsCI	GGATG	8 cut(s)	27, 225, 433, 697, 886, 911, 1009, 1137
BtsI	GCAGTG	2 cut(s)	211, 998
BtsIMutI	CAGTG	3 cut(s)	211, 363, 998
Cac8I	GCNNGC	2 cut(s)	49, 355
CaiI	CAGNNNCTG	1 cut(s)	892
CciI	TCATGA	1 cut(s)	445
Cfr10I	RCCGGY	1 cut(s)	75
Cfr13I	GGNCC	2 cut(s)	112, 575
CseI	GACGC	1 cut(s)	1247
Csp6I	GTAC	2 cut(s)	745, 1299
CviQI	GTAC	2 cut(s)	745, 1299
DdeI	CTNAG	3 cut(s)	456, 1149, 1295
DpnI	GATC	4 cut(s)	213, 661, 864, 1196
DpnII	GATC	4 cut(s)	211, 659, 862, 1194
DrdI	GACNNNNNNGTC	1 cut(s)	1257
DseDI	GACNNNNNNGTC	1 cut(s)	1257
EaeI	YGGCCR	1 cut(s)	786
Eam1104I	CTCTTC	1 cut(s)	645
EarI	CTCTTC	1 cut(s)	645
Eco130I	CCWWGG	2 cut(s)	948, 1130
Eco147I	AGGCCT	1 cut(s)	1203
Eco47I	GGWCC	1 cut(s)	112
Eco57I	CTGAAG	2 cut(s)	1184, 1247
Eco91I	GGTNACC	1 cut(s)	1233
EcoO65I	GGTNACC	1 cut(s)	1233
EcoRII	CCWGG	1 cut(s)	788
EcoT14I	CCWWGG	2 cut(s)	948, 1130
ErhI	CCWWGG	2 cut(s)	948, 1130
FaqI	GGGAC	3 cut(s)	154, 346, 769
FauNDI	CATATG	3 cut(s)	520, 532, 829
FbaI	TGATCA	1 cut(s)	862
FblI	GTMKAC	1 cut(s)	282
Fnu4HI	GCNGC	2 cut(s)	45, 267
FokI	GGATG	8 cut(s)	14, 212, 440, 704, 893, 918, 996, 1124
Fsp4HI	GCNGC	2 cut(s)	45, 267
FspBI	CTAG	2 cut(s)	347, 1101
GluI	GCNGC	2 cut(s)	45, 267
GsuI	CTGGAG	2 cut(s)	60, 516
HaeIII	GGCC	4 cut(s)	576, 738, 788, 1203
HapII	CCGG	3 cut(s)	76, 85, 1032
HgaI	GACGC	1 cut(s)	1247
Hin1I	GRCGYC	1 cut(s)	1258
HincII	GTYRAC	2 cut(s)	220, 283
HindII	GTYRAC	2 cut(s)	220, 283
HindIII	AAGCTT	3 cut(s)	122, 351, 898
HinfI	GANTC	6 cut(s)	381, 601, 646, 967, 1070, 1097
HpaII	CCGG	3 cut(s)	76, 85, 1032
HphI	GGTGA	3 cut(s)	304, 665, 740
Hpy166II	GTNNAC	4 cut(s)	220, 283, 312, 745
Hpy188III	TCNNGA	5 cut(s)	85, 347, 446, 964, 1265
Hpy8I	GTNNAC	4 cut(s)	220, 283, 312, 745
HpyAV	CCTTC	3 cut(s)	168, 865, 1214
HpyCH4III	ACNGT	2 cut(s)	134, 1272
HpyCH4V	TGCA	6 cut(s)	72, 259, 266, 357, 485, 628
HpyF10VI	GCNNNNNNNGC	2 cut(s)	491, 634
HpyF3I	CTNAG	3 cut(s)	456, 1149, 1295
Hsp92I	GRCGYC	1 cut(s)	1258
Kpn2I	TCCGGA	1 cut(s)	84
Ksp22I	TGATCA	1 cut(s)	862
Kzo9I	GATC	4 cut(s)	211, 659, 862, 1194
LmnI	GCTCC	4 cut(s)	41, 497, 1008, 1132
Lsp1109I	GCAGC	2 cut(s)	31, 278
LweI	GCATC	2 cut(s)	418, 495
MaeI	CTAG	2 cut(s)	347, 1101
MaeIII	GTNAC	4 cut(s)	296, 361, 1233, 1272
MalI	GATC	4 cut(s)	213, 661, 864, 1196
MboI	GATC	4 cut(s)	211, 659, 862, 1194
MboII	GAAGA	9 cut(s)	34, 157, 335, 455, 632, 772, 955, 1225, 1253
MflI	RGATCY	1 cut(s)	659
MhlI	GDGCHC	1 cut(s)	1000
MlsI	TGGCCA	1 cut(s)	788
MluCI	AATT	5 cut(s)	139, 608, 795, 1035, 1046
MluNI	TGGCCA	1 cut(s)	788
MmeI	TCCRAC	2 cut(s)	491, 891
Mox20I	TGGCCA	1 cut(s)	788
MroI	TCCGGA	1 cut(s)	84
MroXI	GAANNNNTTC	1 cut(s)	714
MscI	TGGCCA	1 cut(s)	788
MseI	TTAA	2 cut(s)	153, 180
MslI	CAYNNNNRTG	3 cut(s)	254, 654, 726
Msp20I	TGGCCA	1 cut(s)	788
MspI	CCGG	3 cut(s)	76, 85, 1032
MspR9I	CCNGG	2 cut(s)	790, 1032
MvaI	CCWGG	1 cut(s)	790
MwoI	GCNNNNNNNGC	2 cut(s)	491, 634
NciI	CCSGG	1 cut(s)	1032
NcoI	CCATGG	1 cut(s)	948
NdeI	CATATG	3 cut(s)	520, 532, 829
NdeII	GATC	4 cut(s)	211, 659, 862, 1194
NlaIV	GGNNCC	4 cut(s)	577, 661, 1029, 1057
NmuCI	GTSAC	2 cut(s)	361, 1272
NspI	RCATGY	1 cut(s)	341
PagI	TCATGA	1 cut(s)	445
PceI	AGGCCT	1 cut(s)	1203
PciI	ACATGT	1 cut(s)	337
PdmI	GAANNNNTTC	1 cut(s)	714
PfeI	GAWTC	6 cut(s)	381, 601, 646, 967, 1070, 1097
PflMI	CCANNNNNTGG	1 cut(s)	954
PfoI	TCCNGGA	1 cut(s)	1030
PkrI	GCNGC	2 cut(s)	46, 268
PscI	ACATGT	1 cut(s)	337
PshAI	GACNNNNGTC	1 cut(s)	278
Psp6I	CCWGG	1 cut(s)	788
PspEI	GGTNACC	1 cut(s)	1233
PspGI	CCWGG	1 cut(s)	788
PspN4I	GGNNCC	4 cut(s)	577, 661, 1029, 1057
PspPI	GGNCC	2 cut(s)	112, 575
PstNI	CAGNNNCTG	1 cut(s)	892
PsuI	RGATCY	1 cut(s)	659
RsaI	GTAC	2 cut(s)	746, 1300
RsaNI	GTAC	2 cut(s)	745, 1299
RseI	CAYNNNNRTG	3 cut(s)	254, 654, 726
SalI	GTCGAC	1 cut(s)	281
SaqAI	TTAA	2 cut(s)	153, 180
SatI	GCNGC	2 cut(s)	45, 267
Sau3AI	GATC	4 cut(s)	211, 659, 862, 1194
Sau96I	GGNCC	2 cut(s)	112, 575
ScrFI	CCNGG	2 cut(s)	790, 1032
SduI	GDGCHC	1 cut(s)	1000
SfaNI	GCATC	2 cut(s)	418, 495
SinI	GGWCC	1 cut(s)	112
SmiMI	CAYNNNNRTG	3 cut(s)	254, 654, 726
Sse9I	AATT	5 cut(s)	139, 608, 795, 1035, 1046
SseBI	AGGCCT	1 cut(s)	1203
SspMI	CTAG	2 cut(s)	347, 1101
StuI	AGGCCT	1 cut(s)	1203
StyD4I	CCNGG	2 cut(s)	788, 1030
StyI	CCWWGG	2 cut(s)	948, 1130
TaaI	ACNGT	2 cut(s)	134, 1272
TaqI	TCGA	2 cut(s)	282, 780
TaqII	GACCGA	1 cut(s)	251
TasI	AATT	5 cut(s)	139, 608, 795, 1035, 1046
TatI	WGTACW	1 cut(s)	744
TfiI	GAWTC	6 cut(s)	381, 601, 646, 967, 1070, 1097
Tru1I	TTAA	2 cut(s)	153, 180
Tru9I	TTAA	2 cut(s)	153, 180
TscAI	CASTG	3 cut(s)	211, 370, 1005
TseFI	GTSAC	2 cut(s)	361, 1272
TseI	GCWGC	2 cut(s)	44, 266
Tsp45I	GTSAC	2 cut(s)	361, 1272
TspDTI	ATGAA	8 cut(s)	43, 214, 373, 377, 417, 519, 601, 638
TspRI	CASTG	3 cut(s)	211, 370, 1005
Van91I	CCANNNNNTGG	1 cut(s)	954
VpaK11BI	GGWCC	1 cut(s)	112
XapI	RAATTY	3 cut(s)	139, 608, 795
XbaI	TCTAGA	1 cut(s)	346
XceI	RCATGY	1 cut(s)	341
XmiI	GTMKAC	1 cut(s)	282
XmnI	GAANNNNTTC	1 cut(s)	714
XspI	CTAG	2 cut(s)	347, 1101

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.