AT1G56310

3'-5' exonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0003918
Physical Location & Seq
Forward (+)
21082490 .. 21085651
3162 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G56310.2

Sequence Viewer

Length: 1770 bp
ATGGGTTTGGATTCTAAAGAAGCTGATTTGGAGGTAATAAGAGATGAGAAATCTGAAGCAAACACTGTGTGTTTACATGCGTTTTCAGATTTAACCTATGTGTCTCCTGTTGTGTTCTTATACCTACTCAAAGAATGCTATAAACATGGTAGCTTGAAGGCAACAAAAAAGTTCCAAGCTTTACAGTATCAAGTTCATCGAGTTCTAGCTAATAAACCTCAACCAGGACCTGCTACTTTCATTATTAATTGTCTCACTTTACTTCCTTTATTTGGGGTATATGGTGAAGGCTTTAGTCATTTAGTTATATCAGCTCTTCGCCGCTTCTTTAAAACAGTATCTGAACCAACTAGTGAAGAAGATATTTGTTTGGCGAGAAAGCTAGCTGCTCAGTTCTTCCTTGCTACTGTTGGTGGATCTTTAACTTATGATGAGAAGGTTATGGTGCATACTCTTAGAGTGTTTGATGTGAGGTTAACTAGTATCGATGAAGCCTTGTCTATCTCGGAAGTTTGGCAGAGATATGGGTTTGCTTGTGGAAATGCGTTTCTGGAACAATACATTTCTGACTTGATCAAGTCGAAATCTTTCATGACGGCTGTGACTCTGTTAGAGCATTTCTCTTTCCGTTTCCCTGGAGAAACTTTTCTTCAACAAATGGTTGAGGATAAAAATTTCCAAGCTGCAGAGAGATGGGCTACCTTCATGGGAAGGCCAAGTTTATGCATTCTTGTTCAAGAGTATGGCTCAAGGAATATGCTAAAGCAGGCCTATAATATCATAAATAAGAACTATCTACAGCATGACTTTCCCGAATTGTATCACAAGTGTAAAGAAAGTGCTCTGAAGGTTCTAGCAGAAAAAGCATGTTGGGATGTTGCTGAAATTAAGACAAAAGGTGATAGACAGCTTCTGAAGTATCTGGTATACTTGGCAGTGGAAGCTGGATACTTGGAGAAGGTTGATGAACTGTGCGATCGATATTCACTTCAAGGGCTGCCAAAAGCACGAGAGGCTGAGGTTGCTTTTGTTGAAAAAAGCTTTCTGCGTCTCAACGATCTAGCTGTAGAAGATGTAGTTTGGGTTGATGAAGTCAACGAGTTGAGAAAAGCAACTTCTTTTCTTGAAGGATGTAGAGTTGTGGGTATTGACTGTGAATGGAAACCTAATTATATTAAAGGCAGTAAACAGAACAAGGTTTCAATCATGCAAATTGGATCTGATACCAAAATTTTCATATTGGACTTGATAAAGCTTTACAATGACGCCTCTGAAATTCTGGACAACTGCCTTAGTCACATTTTGCAATCGAAGAGTACATTAAAGCTCGGTTACAATTTTCAATGTGACATCAAGCAGTTGGCGCTTTCATATGGGGATTTGAAATGTTTCGAGCGATACGACATGTTGCTAGACATTCAAAATGTTTTTAATGAACCATTTGGTGGTTTAGCAGGACTAACGAAGAAAATATTGGGAGTGTCTTTGAACAAAACAAGACGCAATAGCGACTGGGAACAAAGGCCTTTGAGCCAGAATCAGCTTGAGTATGCTGCTCTTGATGCTGCAGTGTTGATTCACATATTTCGCCATGTTCGCGATCATCCTCCACATGACAGTAGTTCAGAGACAACCCAGTGGAAATCTCACATTGTCTCTCACATGGAGAGCCCAAAGAAGTTGAAGAAGAAGACGCCTAAGAGCAATCCCACGCAAACATCAGCCTCGACATTTAACAAGCCATCAGACAATTGGAACAAATGGAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_pol_A_exo1

Protein Analysis

589

Amino Acids

67.29

Weight (kDa)

7.52

Isoelectric Point (pI)

38.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_pol_A_exo1 PF01612 360 - 531 3e-22 3'-5' exonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 238
AccB7I CCANNNNNTGG 1 cut(s) 1445
AccI GTMKAC 1 cut(s) 927
AccII CGCG 1 cut(s) 1599
AciI CCGC 1 cut(s) 322
AclWI GGATC 2 cut(s) 424, 1225
AcsI RAATTY 3 cut(s) 673, 1230, 1275
AcuI CTGAAG 3 cut(s) 75, 866, 935
AcyI GRCGYC 2 cut(s) 1266, 1694
AdeI CACNNNGTG 1 cut(s) 69
AfaI GTAC 1 cut(s) 1318
AfiI CCNNNNNNNGG 3 cut(s) 224, 272, 1445
AflIII ACRYGT 1 cut(s) 1404
AhlI ACTAGT 2 cut(s) 350, 479
AjnI CCWGG 2 cut(s) 223, 634
AjuI GAANNNNNNNTTGG 2 cut(s) 1457, 1489
Alw21I GWGCWC 1 cut(s) 844
Alw26I GTCTC 5 cut(s) 108, 257, 1055, 1622, 1660
AlwI GGATC 2 cut(s) 424, 1225
AlwNI CAGNNNCTG 3 cut(s) 230, 341, 913
AoxI GGCC 3 cut(s) 713, 768, 1523
ApeKI GCWGC 5 cut(s) 386, 683, 997, 1553, 1565
ApoI RAATTY 3 cut(s) 673, 1230, 1275
AseI ATTAAT 1 cut(s) 246
Asp700I GAANNNNTTC 3 cut(s) 587, 645, 1388
AspLEI GCGC 1 cut(s) 1366
AspS9I GGNCC 1 cut(s) 227
AsuHPI GGTGA 2 cut(s) 296, 911
AsuNHI GCTAGC 1 cut(s) 382
AvaII GGWCC 1 cut(s) 227
BanII GRGCYC 1 cut(s) 1673
BauI CACGAG 1 cut(s) 1008
BbsI GAAGAC 1 cut(s) 1697
Bbv12I GWGCWC 1 cut(s) 844
BbvCI CCTCAGC 1 cut(s) 1017
BbvI GCAGC 5 cut(s) 373, 670, 984, 1540, 1552
BccI CCATC 2 cut(s) 687, 1750
BceAI ACGGC 1 cut(s) 612
BciT130I CCWGG 2 cut(s) 225, 636
BciVI GTATCC 1 cut(s) 941
BclI TGATCA 1 cut(s) 573
BcoDI GTCTC 5 cut(s) 108, 257, 1055, 1622, 1660
BcuI ACTAGT 2 cut(s) 350, 479
BfaI CTAG 8 cut(s) 206, 351, 383, 480, 854, 1061, 1412, 1768
BfmI CTRYAG 4 cut(s) 684, 797, 1065, 1566
BfoI RGCGCY 1 cut(s) 1367
BfuAI ACCTGC 1 cut(s) 238
BfuI GTATCC 1 cut(s) 941
BisI GCNGC 6 cut(s) 322, 387, 684, 998, 1554, 1566
BlsI GCNGC 6 cut(s) 323, 388, 685, 999, 1555, 1567
Bme1390I CCNGG 2 cut(s) 225, 636
Bme18I GGWCC 1 cut(s) 227
BmgT120I GGNCC 1 cut(s) 227
BmrFI CCNGG 2 cut(s) 225, 636
BmrI ACTGGG 2 cut(s) 1522, 1630
BmsI GCATC 1 cut(s) 1552
BmtI GCTAGC 1 cut(s) 386
BmuI ACTGGG 2 cut(s) 1522, 1630
BpiI GAAGAC 1 cut(s) 1697
BplI GAGNNNNNCTC 4 cut(s) 605, 637, 731, 763
BpmI CTGGAG 1 cut(s) 657
Bpu10I CCTNAGC 1 cut(s) 1017
BpuEI CTTGAG 2 cut(s) 733, 1565
Bsa29I ATCGAT 2 cut(s) 486, 979
BsaHI GRCGYC 2 cut(s) 1266, 1694
BsaJI CCNNGG 1 cut(s) 634
Bsc4I CCNNNNNNNGG 3 cut(s) 224, 272, 1445
Bse1I ACTGG 2 cut(s) 1517, 1636
BseBI CCWGG 2 cut(s) 225, 636
BseCI ATCGAT 2 cut(s) 486, 979
BseDI CCNNGG 1 cut(s) 634
BseGI GGATG 3 cut(s) 880, 1136, 1603
BseLI CCNNNNNNNGG 3 cut(s) 224, 272, 1445
BseMII CTCAG 2 cut(s) 404, 1008
BseNI ACTGG 2 cut(s) 1517, 1636
BseXI GCAGC 5 cut(s) 373, 670, 984, 1540, 1552
Bsh1236I CGCG 1 cut(s) 1599
Bsh1285I CGRYCG 1 cut(s) 979
BshFI GGCC 3 cut(s) 715, 770, 1525
BshVI ATCGAT 2 cut(s) 486, 979
BsiEI CGRYCG 1 cut(s) 979
BsiHKAI GWGCWC 1 cut(s) 844
BslI CCNNNNNNNGG 3 cut(s) 224, 272, 1445
BsmAI GTCTC 5 cut(s) 108, 257, 1055, 1622, 1660
BsmBI CGTCTC 1 cut(s) 1055
BsmI GAATGC 2 cut(s) 140, 726
BsnI GGCC 3 cut(s) 715, 770, 1525
Bsp1286I GDGCHC 2 cut(s) 844, 1673
Bsp143I GATC 6 cut(s) 416, 573, 976, 1057, 1217, 1600
Bsp68I TCGCGA 1 cut(s) 1599
BspACI CCGC 1 cut(s) 322
BspANI GGCC 3 cut(s) 715, 770, 1525
BspCNI CTCAG 2 cut(s) 403, 1009
BspDI ATCGAT 2 cut(s) 486, 979
BspFNI CGCG 1 cut(s) 1599
BspHI TCATGA 1 cut(s) 591
BspMAI CTGCAG 2 cut(s) 688, 1570
BspMI ACCTGC 1 cut(s) 238
BspOI GCTAGC 1 cut(s) 386
BspPI GGATC 2 cut(s) 424, 1225
BspQI GCTCTTC 1 cut(s) 321
BsrI ACTGG 2 cut(s) 1517, 1636
BssECI CCNNGG 1 cut(s) 634
BssMI GATC 6 cut(s) 416, 573, 976, 1057, 1217, 1600
BssNAI GTATAC 1 cut(s) 928
BssNI GRCGYC 2 cut(s) 1266, 1694
BssSI CACGAG 1 cut(s) 1008
Bst1107I GTATAC 1 cut(s) 928
Bst2BI CACGAG 1 cut(s) 1008
Bst2UI CCWGG 2 cut(s) 225, 636
Bst4CI ACNGT 7 cut(s) 67, 186, 337, 409, 972, 1154, 1619
Bst6I CTCTTC 2 cut(s) 321, 1307
BstACI GRCGYC 2 cut(s) 1266, 1694
BstC8I GCNNGC 2 cut(s) 384, 768
BstDEI CTNAG 5 cut(s) 390, 455, 1017, 1292, 1698
BstENI CCTNNNNNAGG 1 cut(s) 222
BstF5I GGATG 3 cut(s) 880, 1136, 1603
BstFNI CGCG 1 cut(s) 1599
BstH2I RGCGCY 1 cut(s) 1367
BstHHI GCGC 1 cut(s) 1366
BstKTI GATC 6 cut(s) 419, 576, 979, 1060, 1220, 1603
BstMAI GTCTC 5 cut(s) 108, 257, 1055, 1622, 1660
BstMBI GATC 6 cut(s) 416, 573, 976, 1057, 1217, 1600
BstMCI CGRYCG 1 cut(s) 979
BstMWI GCNNNNNNNGC 7 cut(s) 863, 941, 1013, 1022, 1363, 1562, 1596
BstNI CCWGG 2 cut(s) 225, 636
BstNSI RCATGY 3 cut(s) 80, 870, 1408
BstSCI CCNGG 2 cut(s) 223, 634
BstSFI CTRYAG 4 cut(s) 684, 797, 1065, 1566
BstUI CGCG 1 cut(s) 1599
BstV1I GCAGC 5 cut(s) 373, 670, 984, 1540, 1552
BstV2I GAAGAC 1 cut(s) 1697
BstX2I RGATCY 2 cut(s) 416, 1217
BstYI RGATCY 2 cut(s) 416, 1217
BstZ17I GTATAC 1 cut(s) 928
Bsu15I ATCGAT 2 cut(s) 486, 979
BsuI GTATCC 1 cut(s) 941
BsuRI GGCC 3 cut(s) 715, 770, 1525
BsuTUI ATCGAT 2 cut(s) 486, 979
BtsCI GGATG 3 cut(s) 880, 1136, 1603
BtsI GCAGTG 2 cut(s) 942, 1575
BtsIMutI CAGTG 4 cut(s) 63, 942, 1575, 1643
BtuMI TCGCGA 1 cut(s) 1599
BveI ACCTGC 1 cut(s) 238
Cac8I GCNNGC 2 cut(s) 384, 768
CaiI CAGNNNCTG 3 cut(s) 230, 341, 913
CciI TCATGA 1 cut(s) 591
CfoI GCGC 1 cut(s) 1366
Cfr13I GGNCC 1 cut(s) 227
ClaI ATCGAT 2 cut(s) 486, 979
CseI GACGC 4 cut(s) 1037, 1274, 1509, 1702
Csp6I GTAC 1 cut(s) 1317
CviQI GTAC 1 cut(s) 1317
DdeI CTNAG 5 cut(s) 390, 455, 1017, 1292, 1698
DpnI GATC 6 cut(s) 418, 575, 978, 1059, 1219, 1602
DpnII GATC 6 cut(s) 416, 573, 976, 1057, 1217, 1600
DraI TTTAAA 1 cut(s) 331
DraIII CACNNNGTG 1 cut(s) 69
Eam1104I CTCTTC 2 cut(s) 321, 1307
EarI CTCTTC 2 cut(s) 321, 1307
Eco147I AGGCCT 2 cut(s) 770, 1525
Eco24I GRGCYC 1 cut(s) 1673
Eco47I GGWCC 1 cut(s) 227
Eco57I CTGAAG 3 cut(s) 75, 866, 935
EcoNI CCTNNNNNAGG 1 cut(s) 222
EcoO109I RGGNCCY 1 cut(s) 227
EcoRII CCWGG 2 cut(s) 223, 634
EcoT22I ATGCAT 1 cut(s) 728
EcoT38I GRGCYC 1 cut(s) 1673
Esp3I CGTCTC 1 cut(s) 1055
FauNDI CATATG 1 cut(s) 1372
FbaI TGATCA 1 cut(s) 573
FblI GTMKAC 1 cut(s) 927
Fnu4HI GCNGC 6 cut(s) 322, 387, 684, 998, 1554, 1566
FokI GGATG 3 cut(s) 887, 1143, 1590
FriOI GRGCYC 1 cut(s) 1673
Fsp4HI GCNGC 6 cut(s) 322, 387, 684, 998, 1554, 1566
FspBI CTAG 8 cut(s) 206, 351, 383, 480, 854, 1061, 1412, 1768
GlaI GCGC 1 cut(s) 1365
GluI GCNGC 6 cut(s) 322, 387, 684, 998, 1554, 1566
GsuI CTGGAG 1 cut(s) 657
HaeII RGCGCY 1 cut(s) 1367
HaeIII GGCC 3 cut(s) 715, 770, 1525
HgaI GACGC 4 cut(s) 1037, 1274, 1509, 1702
HhaI GCGC 1 cut(s) 1366
Hin1I GRCGYC 2 cut(s) 1266, 1694
Hin6I GCGC 1 cut(s) 1364
HinP1I GCGC 1 cut(s) 1364
HincII GTYRAC 2 cut(s) 477, 1096
HindII GTYRAC 2 cut(s) 477, 1096
HindIII AAGCTT 3 cut(s) 177, 1039, 1253
HinfI GANTC 4 cut(s) 11, 604, 1537, 1576
HpaI GTTAAC 1 cut(s) 477
HphI GGTGA 2 cut(s) 296, 911
Hpy166II GTNNAC 5 cut(s) 74, 477, 928, 1096, 1187
Hpy188III TCNNGA 8 cut(s) 551, 592, 737, 812, 1124, 1280, 1559, 1598
Hpy8I GTNNAC 5 cut(s) 74, 477, 928, 1096, 1187
HpyAV CCTTC 8 cut(s) 151, 281, 430, 705, 712, 841, 952, 1121
HpyCH4III ACNGT 7 cut(s) 67, 186, 337, 409, 972, 1154, 1619
HpyCH4V TGCA 6 cut(s) 448, 686, 726, 1210, 1306, 1568
HpyF10VI GCNNNNNNNGC 7 cut(s) 863, 941, 1013, 1022, 1363, 1562, 1596
HpyF3I CTNAG 5 cut(s) 390, 455, 1017, 1292, 1698
Hsp92I GRCGYC 2 cut(s) 1266, 1694
HspAI GCGC 1 cut(s) 1364
Ksp22I TGATCA 1 cut(s) 573
KspAI GTTAAC 1 cut(s) 477
Kzo9I GATC 6 cut(s) 416, 573, 976, 1057, 1217, 1600
LguI GCTCTTC 1 cut(s) 321
LmnI GCTCC 1 cut(s) 1764
Lsp1109I GCAGC 5 cut(s) 373, 670, 984, 1540, 1552
LweI GCATC 1 cut(s) 1552
MaeI CTAG 8 cut(s) 206, 351, 383, 480, 854, 1061, 1412, 1768
MaeIII GTNAC 4 cut(s) 601, 1295, 1331, 1346
MalI GATC 6 cut(s) 418, 575, 978, 1059, 1219, 1602
MboI GATC 6 cut(s) 416, 573, 976, 1057, 1217, 1600
MfeI CAATTG 1 cut(s) 1750
MflI RGATCY 2 cut(s) 416, 1217
MhlI GDGCHC 2 cut(s) 844, 1673
MlyI GAGTC 1 cut(s) 598
MnlI CCTC 9 cut(s) 25, 228, 465, 658, 1006, 1012, 1279, 1617, 1735
Mph1103I ATGCAT 1 cut(s) 728
MroXI GAANNNNTTC 3 cut(s) 587, 645, 1388
MspR9I CCNGG 2 cut(s) 225, 636
MunI CAATTG 1 cut(s) 1750
Mva1269I GAATGC 2 cut(s) 140, 726
MvaI CCWGG 2 cut(s) 225, 636
MvnI CGCG 1 cut(s) 1599
MwoI GCNNNNNNNGC 7 cut(s) 863, 941, 1013, 1022, 1363, 1562, 1596
NdeI CATATG 1 cut(s) 1372
NdeII GATC 6 cut(s) 416, 573, 976, 1057, 1217, 1600
NheI GCTAGC 1 cut(s) 382
NmuCI GTSAC 3 cut(s) 601, 1295, 1346
NruI TCGCGA 1 cut(s) 1599
NsiI ATGCAT 1 cut(s) 728
NspI RCATGY 3 cut(s) 80, 870, 1408
PagI TCATGA 1 cut(s) 591
PceI AGGCCT 2 cut(s) 770, 1525
PciI ACATGT 1 cut(s) 1404
PciSI GCTCTTC 1 cut(s) 321
PcsI WCGNNNNNNNCGW 1 cut(s) 1398
PctI GAATGC 2 cut(s) 140, 726
PdmI GAANNNNTTC 3 cut(s) 587, 645, 1388
PfeI GAWTC 3 cut(s) 11, 1537, 1576
PflMI CCANNNNNTGG 1 cut(s) 1445
PkrI GCNGC 6 cut(s) 323, 388, 685, 999, 1555, 1567
Ple19I CGATCG 1 cut(s) 979
PleI GAGTC 1 cut(s) 598
PpsI GAGTC 1 cut(s) 598
PpuMI RGGWCCY 1 cut(s) 227
PscI ACATGT 1 cut(s) 1404
PshBI ATTAAT 1 cut(s) 246
Psp5II RGGWCCY 1 cut(s) 227
Psp6I CCWGG 2 cut(s) 223, 634
PspGI CCWGG 2 cut(s) 223, 634
PspPI GGNCC 1 cut(s) 227
PspPPI RGGWCCY 1 cut(s) 227
PstI CTGCAG 2 cut(s) 688, 1570
PstNI CAGNNNCTG 3 cut(s) 230, 341, 913
PsuI RGATCY 2 cut(s) 416, 1217
PvuI CGATCG 1 cut(s) 979
RruI TCGCGA 1 cut(s) 1599
RsaI GTAC 1 cut(s) 1318
RsaNI GTAC 1 cut(s) 1317
SapI GCTCTTC 1 cut(s) 321
SatI GCNGC 6 cut(s) 322, 387, 684, 998, 1554, 1566
Sau3AI GATC 6 cut(s) 416, 573, 976, 1057, 1217, 1600
Sau96I GGNCC 1 cut(s) 227
SchI GAGTC 1 cut(s) 598
ScrFI CCNGG 2 cut(s) 225, 636
SduI GDGCHC 2 cut(s) 844, 1673
SfaNI GCATC 1 cut(s) 1552
SfcI CTRYAG 4 cut(s) 684, 797, 1065, 1566
SinI GGWCC 1 cut(s) 227
SmlI CTYRAG 2 cut(s) 748, 1544
SmoI CTYRAG 2 cut(s) 748, 1544
SpeI ACTAGT 2 cut(s) 350, 479
SseBI AGGCCT 2 cut(s) 770, 1525
SsiI CCGC 1 cut(s) 322
SspI AATATT 1 cut(s) 1473
SspMI CTAG 8 cut(s) 206, 351, 383, 480, 854, 1061, 1412, 1768
StuI AGGCCT 2 cut(s) 770, 1525
StyD4I CCNGG 2 cut(s) 223, 634
TaaI ACNGT 7 cut(s) 67, 186, 337, 409, 972, 1154, 1619
TaqI TCGA 7 cut(s) 199, 486, 581, 979, 1310, 1392, 1727
TatI WGTACW 1 cut(s) 1316
TauI GCSGC 1 cut(s) 324
TfiI GAWTC 3 cut(s) 11, 1537, 1576
TscAI CASTG 4 cut(s) 70, 942, 1575, 1643
TseFI GTSAC 3 cut(s) 601, 1295, 1346
TseI GCWGC 5 cut(s) 386, 683, 997, 1553, 1565
Tsp45I GTSAC 3 cut(s) 601, 1295, 1346
TspGWI ACGGA 1 cut(s) 617
TspRI CASTG 4 cut(s) 70, 942, 1575, 1643
Van91I CCANNNNNTGG 1 cut(s) 1445
VpaK11BI GGWCC 1 cut(s) 227
VspI ATTAAT 1 cut(s) 246
XagI CCTNNNNNAGG 1 cut(s) 222
XapI RAATTY 3 cut(s) 673, 1230, 1275
XceI RCATGY 3 cut(s) 80, 870, 1408
XcmI CCANNNNNNNNNTGG 1 cut(s) 1749
XmiI GTMKAC 1 cut(s) 927
XmnI GAANNNNTTC 3 cut(s) 587, 645, 1388
XspI CTAG 8 cut(s) 206, 351, 383, 480, 854, 1061, 1412, 1768
Zsp2I ATGCAT 1 cut(s) 728
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.