FvH4_6g07170

3'-5' exonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0003918
Physical Location & Seq
Forward (+)
4317743 .. 4322381
4639 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g07170.t1

Sequence Viewer

Length: 1689 bp
ATGGGTTTGGAGGAGAGGGCTGCCACTTATGACAGCCAGAACGGCAAAGGTGATCAGAGTCATCAGACTATTTGCAAGCATGGATTTTCCGACCTTTCGCACATTTCTCCGGTGGTGTTCTTATACCTCTTGAAAGAATGTTACTTTATTGGCACATGCAAGGCAACTAAGAAGTTCCGAGCTCTTCAAGAACAGGTCCATCTAGTGCTGCGCAATGACCCCCAACCTGGACCAGCTACTTTTGTTGTCCAGTGCCTCTATGTCTTGCCTATTTTCGACTTATACTGTGAAGGGTTCAGTCACTTAATTATATCTGCTCTACGCCGTTTCCTAAAAGAAGAAGCTTCCGTGGGGGACAATTTGGAACCAAAAGACCTTGCTGCCAAGTTATTTCTAGATATTGTTGGGGGCTCTGTCAACCATGATGAGAGGATAGTTGAGAAGATACTTGAAGTTTTTGATGTTCAGTTAGAGAACATTCAAAAAGCTCTGTGCCCAAATGCAATGAATGATGACAGTTTTAGCATGGCAACAAAATTTGTTGAGGATTATATATATCACTTGATAGAGTGTCAGACATACATGACTGCAGTTACTCTATTGGCGCACTTCTGTATCCGCCAGTCTGGTCAATCTTTTCTGCTTTCTATGATAGAAAGCAAACAATTGAAAGCAGCAGAGAAGTGGGCAACCTTCATCGGGAAGCCAATGTTATGTGTTCTGGTCCAAGAGTATTCTGATAGAAACATGCTGCAGGATGCTTATGAGATTATACACAAAAACAATCTCCGACAGGAATTCCCAAATGTTTGTCACAAGTATAAAGAGAGTTCATTAAAGAAATTAGCAGAAAAAGGATGCTGGGACGTTGCAGAAGCAAAGACAAAGAGTGATAGACAACTCCTTGAATACCTGGTTTATTTAGCCATGGAAGCTGGTTACACAGAAAAGGTTGATGAACTATGTGATCGATATTCACTACAAGGTTTCTTGAATGTCAAAGTGCCTGAGCCAAGCCTTCTCCATAATCGCTATTTGCATCTGAATGAGTTAGCTGTAGGAGGTGTCTTTTGGGTTGATGAAGTTGATGCTTTGCATAAGGCGACCTGCCATTTTGAGGAATGCAAAGTTGTGGGTCTTGATTGTGAATGGAAACCGAATTATGTTAAAGGTAGCAGACCAAACAAGGTTTCTATTATGCAAATTGCTTCTGATAAAATGGTTTTCATCCTTGACTTGATCAAATTATATCAAGATGTACCTGATGTTCTGGATGACAGCTTAACTCGTGTTCTACAGTCTCCTGGGATCCTAAAGCTTGGTTATAATTTTCAATGCGATATGAGGCAGCTGGCTCAGTCATATGAAGATTTAGAGTGTTTCAAGCACTATCAGATGCTACTGGACGTTCAGAATGTGTTCAAAGAACCTCGTGGTGGTCTGTCCGGGCTTGCAAAGAAAATACTCGGAGTTGGTTTGAACAAGACAAGAAGGAACAGCAATTGGGAGCAACGGCCTCTGACACCCAATCAGCTGGAATATGCAGCTCTTGATGCAGTTGTACTGGTTCACATATTTGGCCACGTTCGCACTCATTCTCAGCCTGCTGATGGAGAGGGACATGGAAAACTTGAGTGGAAATCCTGCATTGTTTCCAACATGACTAACATGAAGAAGTTCAAAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_pol_A_exo1

Protein Analysis

563

Amino Acids

64.18

Weight (kDa)

6.27

Isoelectric Point (pI)

43.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_pol_A_exo1 PF01612 358 - 528 7.1e-22 3'-5' exonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1326
Acc16I TGCGCA 1 cut(s) 212
Acc36I ACCTGC 1 cut(s) 1115
AciI CCGC 1 cut(s) 619
AclWI GGATC 2 cut(s) 1303, 1316
AcoI YGGCCR 1 cut(s) 1579
AcsI RAATTY 2 cut(s) 536, 797
AfaI GTAC 2 cut(s) 1260, 1563
AfiI CCNNNNNNNGG 5 cut(s) 227, 699, 1117, 1436, 1610
AjnI CCWGG 3 cut(s) 226, 912, 1303
AjuI GAANNNNNNNTTGG 2 cut(s) 1174, 1206
Alw21I GWGCWC 1 cut(s) 184
Alw26I GTCTC 1 cut(s) 1305
AlwI GGATC 2 cut(s) 1303, 1316
AoxI GGCC 2 cut(s) 1514, 1579
ApeKI GCWGC 7 cut(s) 20, 208, 380, 674, 751, 1348, 1544
ApoI RAATTY 2 cut(s) 536, 797
Asp700I GAANNNNTTC 2 cut(s) 1418, 1676
AspLEI GCGC 2 cut(s) 213, 607
AspS9I GGNCC 3 cut(s) 196, 230, 724
AsuC2I CCSGG 1 cut(s) 1447
AsuHPI GGTGA 1 cut(s) 62
AvaII GGWCC 3 cut(s) 196, 230, 724
BaeGI GKGCMC 1 cut(s) 497
BaeI ACNNNNGTAYC 2 cut(s) 598, 631
BalI TGGCCA 1 cut(s) 1581
BamHI GGATCC 1 cut(s) 1308
BanII GRGCYC 2 cut(s) 184, 413
BauI CACGAG 2 cut(s) 1287, 1431
Bbv12I GWGCWC 1 cut(s) 184
BbvI GCAGC 7 cut(s) 7, 195, 367, 686, 738, 1360, 1556
BccI CCATC 2 cut(s) 207, 1604
BceAI ACGGC 3 cut(s) 58, 309, 1529
BciT130I CCWGG 3 cut(s) 228, 914, 1305
BciVI GTATCC 1 cut(s) 626
BclI TGATCA 2 cut(s) 52, 1239
BcnI CCSGG 1 cut(s) 1447
BcoDI GTCTC 1 cut(s) 1305
BfaI CTAG 2 cut(s) 203, 395
BfmI CTRYAG 4 cut(s) 588, 752, 1056, 1295
BfuAI ACCTGC 1 cut(s) 1115
BfuI GTATCC 1 cut(s) 626
BglI GCCNNNNNGGC 1 cut(s) 42
BisI GCNGC 7 cut(s) 21, 209, 381, 675, 752, 1349, 1545
BlsI GCNGC 7 cut(s) 22, 210, 382, 676, 753, 1350, 1546
Bme1390I CCNGG 4 cut(s) 228, 914, 1305, 1447
Bme18I GGWCC 3 cut(s) 196, 230, 724
BmgT120I GGNCC 3 cut(s) 196, 230, 724
BmiI GGNNCC 2 cut(s) 366, 1310
BmrFI CCNGG 4 cut(s) 228, 914, 1305, 1447
BmsI GCATC 6 cut(s) 748, 848, 1048, 1078, 1386, 1543
Bpu10I CCTNAGC 1 cut(s) 1008
BpuEI CTTGAG 1 cut(s) 1652
BpuMI CCSGG 1 cut(s) 1447
Bsa29I ATCGAT 1 cut(s) 970
BsaJI CCNNGG 3 cut(s) 348, 927, 1304
BsaWI WCCGGW 1 cut(s) 109
Bsc4I CCNNNNNNNGG 5 cut(s) 227, 699, 1117, 1436, 1610
Bse1I ACTGG 4 cut(s) 250, 622, 1407, 1569
Bse3DI GCAATG 2 cut(s) 220, 510
BseBI CCWGG 3 cut(s) 228, 914, 1305
BseCI ATCGAT 1 cut(s) 970
BseDI CCNNGG 3 cut(s) 348, 927, 1304
BseGI GGATG 4 cut(s) 763, 863, 1227, 1279
BseLI CCNNNNNNNGG 5 cut(s) 227, 699, 1117, 1436, 1610
BseMI GCAATG 2 cut(s) 220, 510
BseMII CTCAG 3 cut(s) 999, 1370, 1613
BseNI ACTGG 4 cut(s) 250, 622, 1407, 1569
BseRI GAGGAG 1 cut(s) 26
BseSI GKGCMC 1 cut(s) 497
BseXI GCAGC 7 cut(s) 7, 195, 367, 686, 738, 1360, 1556
BseYI CCCAGC 1 cut(s) 861
BshFI GGCC 2 cut(s) 1516, 1581
BshVI ATCGAT 1 cut(s) 970
BsiHKAI GWGCWC 1 cut(s) 184
BsiSI CCGG 2 cut(s) 110, 1446
BslFI GGGAC 3 cut(s) 368, 878, 1632
BslI CCNNNNNNNGG 5 cut(s) 227, 699, 1117, 1436, 1610
BsmAI GTCTC 1 cut(s) 1305
BsmFI GGGAC 3 cut(s) 368, 878, 1632
BsmI GAATGC 1 cut(s) 1127
BsnI GGCC 2 cut(s) 1516, 1581
Bsp1286I GDGCHC 3 cut(s) 184, 413, 497
Bsp143I GATC 4 cut(s) 52, 967, 1239, 1308
Bsp19I CCATGG 1 cut(s) 927
BspACI CCGC 1 cut(s) 619
BspANI GGCC 2 cut(s) 1516, 1581
BspCNI CTCAG 3 cut(s) 1000, 1369, 1612
BspDI ATCGAT 1 cut(s) 970
BspLI GGNNCC 2 cut(s) 366, 1310
BspMAI CTGCAG 2 cut(s) 592, 756
BspMI ACCTGC 1 cut(s) 1115
BspPI GGATC 2 cut(s) 1303, 1316
BspQI GCTCTTC 1 cut(s) 189
BsrDI GCAATG 2 cut(s) 220, 510
BsrI ACTGG 4 cut(s) 250, 622, 1407, 1569
BssECI CCNNGG 3 cut(s) 348, 927, 1304
BssMI GATC 4 cut(s) 52, 967, 1239, 1308
BssSI CACGAG 2 cut(s) 1287, 1431
BssT1I CCWWGG 1 cut(s) 927
Bst2BI CACGAG 2 cut(s) 1287, 1431
Bst2UI CCWGG 3 cut(s) 228, 914, 1305
Bst4CI ACNGT 3 cut(s) 287, 518, 1299
Bst6I CTCTTC 1 cut(s) 189
BstC8I GCNNGC 4 cut(s) 77, 1353, 1452, 1605
BstDEI CTNAG 4 cut(s) 168, 1008, 1356, 1599
BstDSI CCRYGG 2 cut(s) 348, 927
BstF5I GGATG 4 cut(s) 763, 863, 1227, 1279
BstHHI GCGC 2 cut(s) 213, 607
BstKTI GATC 4 cut(s) 55, 970, 1242, 1311
BstMAI GTCTC 1 cut(s) 1305
BstMBI GATC 4 cut(s) 52, 967, 1239, 1308
BstMWI GCNNNNNNNGC 4 cut(s) 42, 932, 1553, 1587
BstNI CCWGG 3 cut(s) 228, 914, 1305
BstNSI RCATGY 2 cut(s) 159, 751
BstSCI CCNGG 4 cut(s) 226, 912, 1303, 1445
BstSFI CTRYAG 4 cut(s) 588, 752, 1056, 1295
BstSLI GKGCMC 1 cut(s) 497
BstV1I GCAGC 7 cut(s) 7, 195, 367, 686, 738, 1360, 1556
BstX2I RGATCY 1 cut(s) 1308
BstXI CCANNNNNNTGG 1 cut(s) 1534
BstYI RGATCY 1 cut(s) 1308
Bsu15I ATCGAT 1 cut(s) 970
BsuI GTATCC 1 cut(s) 626
BsuRI GGCC 2 cut(s) 1516, 1581
BsuTUI ATCGAT 1 cut(s) 970
BtgI CCRYGG 2 cut(s) 348, 927
BtsCI GGATG 4 cut(s) 763, 863, 1227, 1279
BtsIMutI CAGTG 1 cut(s) 257
BveI ACCTGC 1 cut(s) 1115
Cac8I GCNNGC 4 cut(s) 77, 1353, 1452, 1605
CfoI GCGC 2 cut(s) 213, 607
Cfr13I GGNCC 3 cut(s) 196, 230, 724
ClaI ATCGAT 1 cut(s) 970
CsiI ACCWGGT 1 cut(s) 912
Csp6I GTAC 2 cut(s) 1259, 1562
CviQI GTAC 2 cut(s) 1259, 1562
DdeI CTNAG 4 cut(s) 168, 1008, 1356, 1599
DpnI GATC 4 cut(s) 54, 969, 1241, 1310
DpnII GATC 4 cut(s) 52, 967, 1239, 1308
EaeI YGGCCR 1 cut(s) 1579
Eam1104I CTCTTC 1 cut(s) 189
EarI CTCTTC 1 cut(s) 189
EciI GGCGGA 1 cut(s) 608
Ecl136II GAGCTC 1 cut(s) 182
Eco130I CCWWGG 1 cut(s) 927
Eco24I GRGCYC 2 cut(s) 184, 413
Eco47I GGWCC 3 cut(s) 196, 230, 724
Eco53kI GAGCTC 1 cut(s) 182
EcoICRI GAGCTC 1 cut(s) 182
EcoRI GAATTC 1 cut(s) 797
EcoRII CCWGG 3 cut(s) 226, 912, 1303
EcoT14I CCWWGG 1 cut(s) 927
EcoT38I GRGCYC 2 cut(s) 184, 413
ErhI CCWWGG 1 cut(s) 927
FaqI GGGAC 3 cut(s) 368, 878, 1632
FauNDI CATATG 1 cut(s) 1363
FbaI TGATCA 2 cut(s) 52, 1239
Fnu4HI GCNGC 7 cut(s) 21, 209, 381, 675, 752, 1349, 1545
FokI GGATG 4 cut(s) 770, 870, 1214, 1286
FriOI GRGCYC 2 cut(s) 184, 413
Fsp4HI GCNGC 7 cut(s) 21, 209, 381, 675, 752, 1349, 1545
FspBI CTAG 2 cut(s) 203, 395
FspI TGCGCA 1 cut(s) 212
GlaI GCGC 2 cut(s) 212, 606
GluI GCNGC 7 cut(s) 21, 209, 381, 675, 752, 1349, 1545
GsaI CCCAGC 1 cut(s) 865
HaeIII GGCC 2 cut(s) 1516, 1581
HapII CCGG 2 cut(s) 110, 1446
HhaI GCGC 2 cut(s) 213, 607
Hin6I GCGC 2 cut(s) 211, 605
HinP1I GCGC 2 cut(s) 211, 605
HincII GTYRAC 1 cut(s) 418
HindII GTYRAC 1 cut(s) 418
HindIII AAGCTT 2 cut(s) 342, 1316
HinfI GANTC 1 cut(s) 58
HpaII CCGG 2 cut(s) 110, 1446
HphI GGTGA 1 cut(s) 62
Hpy166II GTNNAC 2 cut(s) 418, 1570
Hpy188III TCNNGA 9 cut(s) 130, 188, 395, 700, 991, 1139, 1253, 1271, 1550
Hpy8I GTNNAC 2 cut(s) 418, 1570
HpyAV CCTTC 4 cut(s) 284, 703, 1028, 1485
HpyCH4III ACNGT 3 cut(s) 287, 518, 1299
HpyCH4IV ACGT 3 cut(s) 867, 1407, 1584
HpyF10VI GCNNNNNNNGC 4 cut(s) 42, 932, 1553, 1587
HpyF3I CTNAG 4 cut(s) 168, 1008, 1356, 1599
HpySE526I ACGT 3 cut(s) 867, 1407, 1584
HspAI GCGC 2 cut(s) 211, 605
Ksp22I TGATCA 2 cut(s) 52, 1239
Kzo9I GATC 4 cut(s) 52, 967, 1239, 1308
LguI GCTCTTC 1 cut(s) 189
LmnI GCTCC 1 cut(s) 1507
Lsp1109I GCAGC 7 cut(s) 7, 195, 367, 686, 738, 1360, 1556
LweI GCATC 6 cut(s) 748, 848, 1048, 1078, 1386, 1543
MabI ACCWGGT 1 cut(s) 912
MaeI CTAG 2 cut(s) 203, 395
MaeII ACGT 3 cut(s) 867, 1407, 1584
MaeIII GTNAC 5 cut(s) 140, 299, 592, 812, 938
MalI GATC 4 cut(s) 54, 969, 1241, 1310
MboI GATC 4 cut(s) 52, 967, 1239, 1308
MboII GAAGA 5 cut(s) 176, 350, 454, 1379, 1684
MfeI CAATTG 2 cut(s) 665, 1501
MflI RGATCY 1 cut(s) 1308
MhlI GDGCHC 3 cut(s) 184, 413, 497
MlsI TGGCCA 1 cut(s) 1581
MluNI TGGCCA 1 cut(s) 1581
MlyI GAGTC 1 cut(s) 67
MmeI TCCRAC 3 cut(s) 114, 814, 1680
Mox20I TGGCCA 1 cut(s) 1581
MroXI GAANNNNTTC 2 cut(s) 1418, 1676
MscI TGGCCA 1 cut(s) 1581
MseI TTAA 4 cut(s) 305, 836, 1167, 1283
MslI CAYNNNNRTG 1 cut(s) 1044
Msp20I TGGCCA 1 cut(s) 1581
MspA1I CMGCKG 2 cut(s) 1351, 1534
MspI CCGG 2 cut(s) 110, 1446
MspR9I CCNGG 4 cut(s) 228, 914, 1305, 1447
MunI CAATTG 2 cut(s) 665, 1501
Mva1269I GAATGC 1 cut(s) 1127
MvaI CCWGG 3 cut(s) 228, 914, 1305
MwoI GCNNNNNNNGC 4 cut(s) 42, 932, 1553, 1587
NciI CCSGG 1 cut(s) 1447
NcoI CCATGG 1 cut(s) 927
NdeI CATATG 1 cut(s) 1363
NdeII GATC 4 cut(s) 52, 967, 1239, 1308
NlaIV GGNNCC 2 cut(s) 366, 1310
NmuCI GTSAC 2 cut(s) 299, 812
NsbI TGCGCA 1 cut(s) 212
NspI RCATGY 2 cut(s) 159, 751
PciSI GCTCTTC 1 cut(s) 189
PctI GAATGC 1 cut(s) 1127
PdmI GAANNNNTTC 2 cut(s) 1418, 1676
PkrI GCNGC 7 cut(s) 22, 210, 382, 676, 753, 1350, 1546
PleI GAGTC 1 cut(s) 66
PpsI GAGTC 1 cut(s) 66
PsiI TTATAA 1 cut(s) 1326
Psp124BI GAGCTC 1 cut(s) 184
Psp6I CCWGG 3 cut(s) 226, 912, 1303
PspFI CCCAGC 1 cut(s) 861
PspGI CCWGG 3 cut(s) 226, 912, 1303
PspN4I GGNNCC 2 cut(s) 366, 1310
PspPI GGNCC 3 cut(s) 196, 230, 724
PsrI GAACNNNNNNTAC 2 cut(s) 1251, 1283
PstI CTGCAG 2 cut(s) 592, 756
PsuI RGATCY 1 cut(s) 1308
PvuII CAGCTG 2 cut(s) 1351, 1534
RsaI GTAC 2 cut(s) 1260, 1563
RsaNI GTAC 2 cut(s) 1259, 1562
RseI CAYNNNNRTG 1 cut(s) 1044
SacI GAGCTC 1 cut(s) 184
SapI GCTCTTC 1 cut(s) 189
SaqAI TTAA 4 cut(s) 305, 836, 1167, 1283
SatI GCNGC 7 cut(s) 21, 209, 381, 675, 752, 1349, 1545
Sau3AI GATC 4 cut(s) 52, 967, 1239, 1308
Sau96I GGNCC 3 cut(s) 196, 230, 724
SchI GAGTC 1 cut(s) 67
ScrFI CCNGG 4 cut(s) 228, 914, 1305, 1447
SduI GDGCHC 3 cut(s) 184, 413, 497
SexAI ACCWGGT 1 cut(s) 912
SfaNI GCATC 6 cut(s) 748, 848, 1048, 1078, 1386, 1543
SfcI CTRYAG 4 cut(s) 588, 752, 1056, 1295
SinI GGWCC 3 cut(s) 196, 230, 724
SmiMI CAYNNNNRTG 1 cut(s) 1044
SmlI CTYRAG 1 cut(s) 1631
SmoI CTYRAG 1 cut(s) 1631
SsiI CCGC 1 cut(s) 619
SspMI CTAG 2 cut(s) 203, 395
SstI GAGCTC 1 cut(s) 184
StyD4I CCNGG 4 cut(s) 226, 912, 1303, 1445
StyI CCWWGG 1 cut(s) 927
TaaI ACNGT 3 cut(s) 287, 518, 1299
TaiI ACGT 3 cut(s) 870, 1410, 1587
TaqI TCGA 2 cut(s) 276, 970
TatI WGTACW 1 cut(s) 1561
Tru1I TTAA 4 cut(s) 305, 836, 1167, 1283
Tru9I TTAA 4 cut(s) 305, 836, 1167, 1283
TscAI CASTG 1 cut(s) 257
TseFI GTSAC 2 cut(s) 299, 812
TseI GCWGC 7 cut(s) 20, 208, 380, 674, 751, 1348, 1544
Tsp45I GTSAC 2 cut(s) 299, 812
TspDTI ATGAA 8 cut(s) 521, 685, 822, 972, 1095, 1216, 1380, 1685
TspGWI ACGGA 1 cut(s) 337
TspRI CASTG 1 cut(s) 257
VpaK11BI GGWCC 3 cut(s) 196, 230, 724
XapI RAATTY 2 cut(s) 536, 797
XbaI TCTAGA 1 cut(s) 394
XceI RCATGY 2 cut(s) 159, 751
XmnI GAANNNNTTC 2 cut(s) 1418, 1676
XspI CTAG 2 cut(s) 203, 395
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.