Rh3AG076200

3'-5' exonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0003918
Physical Location & Seq
Forward (+)
6117324 .. 6123069
5746 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG076200.1

Sequence Viewer

Length: 1296 bp
ATGCTTGAAGTTTTTGATGTTCAGTTAGAGAATATTGAAAAAGTTTTGTGCCCAAATGCAATAAAGGATGGCACTTTTAGCATGGCAACAAAATTTGTTGAGGATTATATATATCAGTTGATAGAGTCTCAAACATACATGACTGTGGTTACTCTATTGGCGCACTTCTCTATCCGCCAGTCTGGTGAATCTTTTCTGCTTTGTATGATAGAGAGCCAACAATTGAAAGCAGCAGAGAAGTGGGCAACATTCATCGGAAAGCCAATGTTATGTGTTCTGGTCCAAGAGTATGTTGATAGAAACATGCTAAAGAATGCTTACGAGATTATACACAAAAACAATCTCCGACAGGAATTCCCCGACGTTTATCACAAGTATAAAGAGAGCTCATTAAAGAAACTAGCAGAAAAAGGATGCTGGGATGTTGCAGAAGCAAAGACAAAGAGTAATAGACAACTCCTTGAATACCTGGTTTATTTAGCCATGGAAGCTGGTTACTCGGAAAAGGTTGATGAACTATGTGATCGATATTCACTTCAAGGTTTCTTGAATGCCAAAGTGCCTGAGCCAAGCCTTCTCCATAGTCGCTATTTGCATCTGGATGAGTTAGTTGTAGAAGGTGTCATTTGGGTTGATGAAGTTAATGCTTTGCGTAAGGCAACCTGTCATCTTGAGGAATGCAAAGTTGTTGGTCTTGATTGTGAATGGAAACCGAATTATGTAAAAGGTAGCAAACCAAACAAGGTTTCTATTATGCAAATTGCTTCTGATAAGATGGTTTTCATACTTGACTTGATCAAATTATATGAAGACGTGCCTGATCTTCTGGATGACAGCTTAACTCGTATTCTACAGTCTCCTAGGATCCTAAAACTTGGCTATAATTTTCAATGTGATATGAGGCAGCTGGCTCATTCATATGAAGAGTTAGAGTGTTTCAAGCACTATCAGATGCTACTGGACATTCAGAATGTGTTCAAAGAACCTCGAGGTGGTCTATCCGGGCTTGCAAAGAAAATACTTGGAGTTGGTTTGAACAAGACAAGAAGGAACAGCAATTGGGAGCAACGGCCTCTAACACCCAATCAGCTGGAATATGCAGCTCTCGATGCAGCTGTACTGGTTCACATATTTGGCCACGTTCGCGGTCATTCTCACCCTGCTGATGGAGAGGGACATGGAAAACTGGAGTGGAAATCCTGCATTGTTTCCGACATGAATAACATCAAGAAGTCCAAGAAGGGAAGTCGAAAGGAGTCTTCTGAAGCTCAGATAAAGGAGCATTCACCAATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_pol_A_exo1

Protein Analysis

431

Amino Acids

49.48

Weight (kDa)

6.42

Isoelectric Point (pI)

40.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_pol_A_exo1 PF01612 209 - 380 9.1e-23 3'-5' exonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 987
AccII CGCG 1 cut(s) 1146
AciI CCGC 2 cut(s) 175, 1146
AclWI GGATC 2 cut(s) 859, 872
AcoI YGGCCR 1 cut(s) 1135
AcsI RAATTY 2 cut(s) 92, 353
AcuI CTGAAG 1 cut(s) 1284
AfaI GTAC 1 cut(s) 1119
AfiI CCNNNNNNNGG 2 cut(s) 992, 1166
AjiI CACGTC 1 cut(s) 814
AjnI CCWGG 1 cut(s) 468
AjuI GAANNNNNNNTTGG 2 cut(s) 730, 762
AluBI AGCT 8 cut(s) 387, 491, 837, 907, 1090, 1103, 1115, 1268
AluI AGCT 8 cut(s) 387, 491, 837, 907, 1090, 1103, 1115, 1268
Alw21I GWGCWC 1 cut(s) 389
Alw26I GTCTC 2 cut(s) 132, 861
AlwI GGATC 2 cut(s) 859, 872
Ama87I CYCGRG 1 cut(s) 987
AoxI GGCC 2 cut(s) 1070, 1135
ApeKI GCWGC 4 cut(s) 230, 904, 1100, 1112
ApoI RAATTY 2 cut(s) 92, 353
Asp700I GAANNNNTTC 2 cut(s) 192, 974
AspA2I CCTAGG 1 cut(s) 860
AspLEI GCGC 1 cut(s) 163
AspS9I GGNCC 1 cut(s) 280
AsuC2I CCSGG 1 cut(s) 1003
AsuHPI GGTGA 3 cut(s) 197, 1148, 1278
AvaI CYCGRG 1 cut(s) 987
AvaII GGWCC 1 cut(s) 280
AvrII CCTAGG 1 cut(s) 860
BaeGI GKGCMC 1 cut(s) 53
BalI TGGCCA 1 cut(s) 1137
BamHI GGATCC 1 cut(s) 864
BanII GRGCYC 1 cut(s) 389
BbsI GAAGAC 2 cut(s) 816, 1251
Bbv12I GWGCWC 1 cut(s) 389
BbvI GCAGC 4 cut(s) 242, 916, 1112, 1124
BccI CCATC 3 cut(s) 62, 769, 1160
BceAI ACGGC 1 cut(s) 1085
BciT130I CCWGG 1 cut(s) 470
BclI TGATCA 1 cut(s) 795
BcnI CCSGG 1 cut(s) 1003
BcoDI GTCTC 2 cut(s) 132, 861
BfaI CTAG 2 cut(s) 401, 861
BfmI CTRYAG 1 cut(s) 851
BisI GCNGC 4 cut(s) 231, 905, 1101, 1113
BlnI CCTAGG 1 cut(s) 860
BlsI GCNGC 4 cut(s) 232, 906, 1102, 1114
Bme1390I CCNGG 2 cut(s) 470, 1003
Bme18I GGWCC 1 cut(s) 280
BmeT110I CYCGRG 1 cut(s) 987
BmgBI CACGTC 1 cut(s) 814
BmgT120I GGNCC 1 cut(s) 280
BmiI GGNNCC 1 cut(s) 866
BmrFI CCNGG 2 cut(s) 470, 1003
BmsI GCATC 4 cut(s) 404, 604, 942, 1099
BpiI GAAGAC 2 cut(s) 816, 1251
BpmI CTGGAG 1 cut(s) 1208
Bpu10I CCTNAGC 1 cut(s) 564
BpuEI CTTGAG 1 cut(s) 692
BpuMI CCSGG 1 cut(s) 1003
Bsa29I ATCGAT 1 cut(s) 526
BsaJI CCNNGG 2 cut(s) 483, 860
Bsc4I CCNNNNNNNGG 2 cut(s) 992, 1166
Bse1I ACTGG 4 cut(s) 178, 963, 1125, 1191
BseBI CCWGG 1 cut(s) 470
BseCI ATCGAT 1 cut(s) 526
BseDI CCNNGG 2 cut(s) 483, 860
BseGI GGATG 5 cut(s) 73, 419, 427, 607, 835
BseLI CCNNNNNNNGG 2 cut(s) 992, 1166
BseMII CTCAG 2 cut(s) 555, 1283
BseNI ACTGG 4 cut(s) 178, 963, 1125, 1191
BseSI GKGCMC 1 cut(s) 53
BseXI GCAGC 4 cut(s) 242, 916, 1112, 1124
BseYI CCCAGC 1 cut(s) 417
Bsh1236I CGCG 1 cut(s) 1146
BshFI GGCC 2 cut(s) 1072, 1137
BshVI ATCGAT 1 cut(s) 526
BsiHKAI GWGCWC 1 cut(s) 389
BsiHKCI CYCGRG 1 cut(s) 987
BsiSI CCGG 1 cut(s) 1002
BslFI GGGAC 1 cut(s) 1188
BslI CCNNNNNNNGG 2 cut(s) 992, 1166
BsmAI GTCTC 2 cut(s) 132, 861
BsmFI GGGAC 1 cut(s) 1188
BsmI GAATGC 4 cut(s) 319, 556, 683, 1282
BsnI GGCC 2 cut(s) 1072, 1137
BsoBI CYCGRG 1 cut(s) 987
Bsp1286I GDGCHC 2 cut(s) 53, 389
Bsp143I GATC 4 cut(s) 523, 795, 820, 864
Bsp19I CCATGG 1 cut(s) 483
BspACI CCGC 2 cut(s) 175, 1146
BspANI GGCC 2 cut(s) 1072, 1137
BspCNI CTCAG 2 cut(s) 556, 1282
BspDI ATCGAT 1 cut(s) 526
BspFNI CGCG 1 cut(s) 1146
BspLI GGNNCC 1 cut(s) 866
BspPI GGATC 2 cut(s) 859, 872
BsrI ACTGG 4 cut(s) 178, 963, 1125, 1191
BssECI CCNNGG 2 cut(s) 483, 860
BssMI GATC 4 cut(s) 523, 795, 820, 864
BssT1I CCWWGG 2 cut(s) 483, 860
Bst2UI CCWGG 1 cut(s) 470
Bst4CI ACNGT 2 cut(s) 145, 855
Bst6I CTCTTC 1 cut(s) 918
BstC8I GCNNGC 2 cut(s) 909, 1008
BstDEI CTNAG 2 cut(s) 564, 1269
BstDSI CCRYGG 1 cut(s) 483
BstF5I GGATG 5 cut(s) 73, 419, 427, 607, 835
BstFNI CGCG 1 cut(s) 1146
BstHHI GCGC 1 cut(s) 163
BstKTI GATC 4 cut(s) 526, 798, 823, 867
BstMAI GTCTC 2 cut(s) 132, 861
BstMBI GATC 4 cut(s) 523, 795, 820, 864
BstMWI GCNNNNNNNGC 4 cut(s) 78, 488, 1109, 1143
BstNI CCWGG 1 cut(s) 470
BstNSI RCATGY 1 cut(s) 307
BstSCI CCNGG 2 cut(s) 468, 1001
BstSFI CTRYAG 1 cut(s) 851
BstSLI GKGCMC 1 cut(s) 53
BstUI CGCG 1 cut(s) 1146
BstV1I GCAGC 4 cut(s) 242, 916, 1112, 1124
BstV2I GAAGAC 2 cut(s) 816, 1251
BstX2I RGATCY 1 cut(s) 864
BstXI CCANNNNNNTGG 1 cut(s) 1090
BstYI RGATCY 1 cut(s) 864
Bsu15I ATCGAT 1 cut(s) 526
BsuRI GGCC 2 cut(s) 1072, 1137
BsuTUI ATCGAT 1 cut(s) 526
BtgI CCRYGG 1 cut(s) 483
BtrI CACGTC 1 cut(s) 814
BtsCI GGATG 5 cut(s) 73, 419, 427, 607, 835
Cac8I GCNNGC 2 cut(s) 909, 1008
CfoI GCGC 1 cut(s) 163
Cfr13I GGNCC 1 cut(s) 280
ClaI ATCGAT 1 cut(s) 526
CsiI ACCWGGT 1 cut(s) 468
Csp6I GTAC 1 cut(s) 1118
CviAII CATG 6 cut(s) 82, 139, 304, 484, 1178, 1216
CviQI GTAC 1 cut(s) 1118
DdeI CTNAG 2 cut(s) 564, 1269
DpnI GATC 4 cut(s) 525, 797, 822, 866
DpnII GATC 4 cut(s) 523, 795, 820, 864
EaeI YGGCCR 1 cut(s) 1135
Eam1104I CTCTTC 1 cut(s) 918
EarI CTCTTC 1 cut(s) 918
EciI GGCGGA 1 cut(s) 164
Ecl136II GAGCTC 1 cut(s) 387
Eco130I CCWWGG 2 cut(s) 483, 860
Eco24I GRGCYC 1 cut(s) 389
Eco47I GGWCC 1 cut(s) 280
Eco53kI GAGCTC 1 cut(s) 387
Eco57I CTGAAG 1 cut(s) 1284
Eco88I CYCGRG 1 cut(s) 987
EcoICRI GAGCTC 1 cut(s) 387
EcoRI GAATTC 1 cut(s) 353
EcoRII CCWGG 1 cut(s) 468
EcoT14I CCWWGG 2 cut(s) 483, 860
EcoT38I GRGCYC 1 cut(s) 389
ErhI CCWWGG 2 cut(s) 483, 860
FaeI CATG 6 cut(s) 85, 142, 307, 487, 1181, 1219
FaqI GGGAC 1 cut(s) 1188
FatI CATG 6 cut(s) 81, 138, 303, 483, 1177, 1215
FauNDI CATATG 1 cut(s) 919
FbaI TGATCA 1 cut(s) 795
Fnu4HI GCNGC 4 cut(s) 231, 905, 1101, 1113
FokI GGATG 5 cut(s) 80, 426, 434, 614, 842
FriOI GRGCYC 1 cut(s) 389
Fsp4HI GCNGC 4 cut(s) 231, 905, 1101, 1113
FspBI CTAG 2 cut(s) 401, 861
GlaI GCGC 1 cut(s) 162
GluI GCNGC 4 cut(s) 231, 905, 1101, 1113
GsaI CCCAGC 1 cut(s) 421
GsuI CTGGAG 1 cut(s) 1208
HaeIII GGCC 2 cut(s) 1072, 1137
HapII CCGG 1 cut(s) 1002
HhaI GCGC 1 cut(s) 163
Hin1II CATG 6 cut(s) 85, 142, 307, 487, 1181, 1219
Hin6I GCGC 1 cut(s) 161
HinP1I GCGC 1 cut(s) 161
HinfI GANTC 3 cut(s) 125, 188, 1256
HpaII CCGG 1 cut(s) 1002
HphI GGTGA 3 cut(s) 197, 1148, 1278
Hpy166II GTNNAC 1 cut(s) 1126
Hpy188I TCNGA 9 cut(s) 257, 347, 502, 769, 951, 969, 1213, 1264, 1272
Hpy188III TCNNGA 7 cut(s) 547, 599, 671, 695, 827, 1106, 1228
Hpy8I GTNNAC 1 cut(s) 1126
Hpy99I CGWCG 1 cut(s) 365
HpyAV CCTTC 4 cut(s) 584, 611, 1041, 1234
HpyCH4III ACNGT 2 cut(s) 145, 855
HpyCH4IV ACGT 3 cut(s) 363, 813, 1140
HpyCH4V TGCA 9 cut(s) 59, 428, 595, 681, 757, 1010, 1100, 1112, 1203
HpyF10VI GCNNNNNNNGC 4 cut(s) 78, 488, 1109, 1143
HpyF3I CTNAG 2 cut(s) 564, 1269
HpySE526I ACGT 3 cut(s) 363, 813, 1140
Hsp92II CATG 6 cut(s) 85, 142, 307, 487, 1181, 1219
HspAI GCGC 1 cut(s) 161
Ksp22I TGATCA 1 cut(s) 795
Kzo9I GATC 4 cut(s) 523, 795, 820, 864
LmnI GCTCC 2 cut(s) 1063, 1279
Lsp1109I GCAGC 4 cut(s) 242, 916, 1112, 1124
LweI GCATC 4 cut(s) 404, 604, 942, 1099
MabI ACCWGGT 1 cut(s) 468
MaeI CTAG 2 cut(s) 401, 861
MaeII ACGT 3 cut(s) 363, 813, 1140
MaeIII GTNAC 2 cut(s) 148, 494
MalI GATC 4 cut(s) 525, 797, 822, 866
MboI GATC 4 cut(s) 523, 795, 820, 864
MboII GAAGA 4 cut(s) 815, 821, 935, 1251
MfeI CAATTG 2 cut(s) 221, 1057
MflI RGATCY 1 cut(s) 864
MhlI GDGCHC 2 cut(s) 53, 389
MlsI TGGCCA 1 cut(s) 1137
MluCI AATT 9 cut(s) 92, 221, 353, 715, 759, 800, 883, 1057, 1290
MluNI TGGCCA 1 cut(s) 1137
MlyI GAGTC 2 cut(s) 134, 1265
MmeI TCCRAC 2 cut(s) 370, 1236
MnlI CCTC 7 cut(s) 94, 667, 894, 983, 996, 1083, 1165
Mox20I TGGCCA 1 cut(s) 1137
MroXI GAANNNNTTC 2 cut(s) 192, 974
MscI TGGCCA 1 cut(s) 1137
MseI TTAA 3 cut(s) 392, 642, 839
MslI CAYNNNNRTG 3 cut(s) 143, 600, 918
Msp20I TGGCCA 1 cut(s) 1137
MspA1I CMGCKG 3 cut(s) 907, 1090, 1115
MspI CCGG 1 cut(s) 1002
MspR9I CCNGG 2 cut(s) 470, 1003
MunI CAATTG 2 cut(s) 221, 1057
Mva1269I GAATGC 4 cut(s) 319, 556, 683, 1282
MvaI CCWGG 1 cut(s) 470
MvnI CGCG 1 cut(s) 1146
MwoI GCNNNNNNNGC 4 cut(s) 78, 488, 1109, 1143
NciI CCSGG 1 cut(s) 1003
NcoI CCATGG 1 cut(s) 483
NdeI CATATG 1 cut(s) 919
NdeII GATC 4 cut(s) 523, 795, 820, 864
NlaIII CATG 6 cut(s) 85, 142, 307, 487, 1181, 1219
NlaIV GGNNCC 1 cut(s) 866
NspI RCATGY 1 cut(s) 307
PaeR7I CTCGAG 1 cut(s) 987
PctI GAATGC 4 cut(s) 319, 556, 683, 1282
PdmI GAANNNNTTC 2 cut(s) 192, 974
PfeI GAWTC 1 cut(s) 188
PkrI GCNGC 4 cut(s) 232, 906, 1102, 1114
PleI GAGTC 2 cut(s) 133, 1264
PpsI GAGTC 2 cut(s) 133, 1264
Psp124BI GAGCTC 1 cut(s) 389
Psp6I CCWGG 1 cut(s) 468
PspFI CCCAGC 1 cut(s) 417
PspGI CCWGG 1 cut(s) 468
PspN4I GGNNCC 1 cut(s) 866
PspPI GGNCC 1 cut(s) 280
PspXI VCTCGAGB 1 cut(s) 987
PsuI RGATCY 1 cut(s) 864
PvuII CAGCTG 3 cut(s) 907, 1090, 1115
RsaI GTAC 1 cut(s) 1119
RsaNI GTAC 1 cut(s) 1118
RseI CAYNNNNRTG 3 cut(s) 143, 600, 918
SacI GAGCTC 1 cut(s) 389
SaqAI TTAA 3 cut(s) 392, 642, 839
SatI GCNGC 4 cut(s) 231, 905, 1101, 1113
Sau3AI GATC 4 cut(s) 523, 795, 820, 864
Sau96I GGNCC 1 cut(s) 280
SchI GAGTC 2 cut(s) 134, 1265
ScrFI CCNGG 2 cut(s) 470, 1003
SduI GDGCHC 2 cut(s) 53, 389
SexAI ACCWGGT 1 cut(s) 468
SfaNI GCATC 4 cut(s) 404, 604, 942, 1099
SfcI CTRYAG 1 cut(s) 851
Sfr274I CTCGAG 1 cut(s) 987
SinI GGWCC 1 cut(s) 280
SlaI CTCGAG 1 cut(s) 987
SmiMI CAYNNNNRTG 3 cut(s) 143, 600, 918
SmlI CTYRAG 2 cut(s) 671, 987
SmoI CTYRAG 2 cut(s) 671, 987
Sse9I AATT 9 cut(s) 92, 221, 353, 715, 759, 800, 883, 1057, 1290
SsiI CCGC 2 cut(s) 175, 1146
SspI AATATT 1 cut(s) 34
SspMI CTAG 2 cut(s) 401, 861
SstI GAGCTC 1 cut(s) 389
StyD4I CCNGG 2 cut(s) 468, 1001
StyI CCWWGG 2 cut(s) 483, 860
TaaI ACNGT 2 cut(s) 145, 855
TaiI ACGT 3 cut(s) 366, 816, 1143
TaqI TCGA 4 cut(s) 526, 988, 1107, 1249
TasI AATT 9 cut(s) 92, 221, 353, 715, 759, 800, 883, 1057, 1290
TatI WGTACW 1 cut(s) 1117
TfiI GAWTC 1 cut(s) 188
Tru1I TTAA 3 cut(s) 392, 642, 839
Tru9I TTAA 3 cut(s) 392, 642, 839
TseI GCWGC 4 cut(s) 230, 904, 1100, 1112
TspDTI ATGAA 8 cut(s) 241, 528, 651, 772, 822, 906, 936, 1232
VpaK11BI GGWCC 1 cut(s) 280
XapI RAATTY 2 cut(s) 92, 353
XceI RCATGY 1 cut(s) 307
XhoI CTCGAG 1 cut(s) 987
XmaJI CCTAGG 1 cut(s) 860
XmnI GAANNNNTTC 2 cut(s) 192, 974
XspI CTAG 2 cut(s) 401, 861
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.