MD12G1208000.v1.1

3'-5' exonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0003918
Physical Location & Seq
Reverse (-)
28758622 .. 28763441
4820 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1208000.v1.1.491

Sequence Viewer

Length: 1710 bp
ATGGGGTTGGAGGAAAGAGTTGACAGACATAACAACAAGCAAGGCAAAGTTAATCAGACATCAACTATTTGCAAGCATGCTTTTTCTGACCTGACGCATATTACTCCGTTGGTATTCGTATACCTCTTGAAAGAGTGCTATTTCCATGGCACATGTAAGGCAACTAAGAAGTTCCGAGCTCTTCAAGACCAGGTTTATCTAGTACTACGCAATGATCCCCAACCTGGACCAGCTACTTTTGTTATTCAGTGCCTCTATGTTTTGCCTATATTTGATTTATACTGTGAAGGGTTCAGTCATTTGATTATATCTGGTCTTCTCCATTTCCTAAAAAAGGAAACATGCTCGGGGGACTCTATGGAAGCAAAAGACCTCGCTGCCCAGTTATTTCTCGATATTGTTGGGGGCTCTGTCAACCATGAGGGGAGGATAATTGAGAAGATACTTGAAGTTTATGGTGTTCAGTTGGTAAACATTGATAAAGTAATGTGCCTGAATGCAAAGAATAATTGCAGATTTGACAATGCAAAAGCGTTTGTTGAGCAATATATATTTGGACTGATAGAGTCTCAATCATATATGACCGCGGTTACTTTATTAGAGCACTTCTCTATCCGCCAGTCTGGCCAATCGTTTCTCCTTCGTATGATAGAAAGCAGTCAATTGAAAGCAGCAGAAAAGTGGGCAACATTCATGGGAAAGCCAATGTTGTGTATTCTTATCCGGGAGTACTTCGGCAGAAACATGCTAAAAAATGCTTATGACATTATACAAAAAAACAATCTCCGACATGAATTTCCTGATGTTTATCATAAGTACAAAGAGAGCACTTTAAAGAAACTAGCAGAAAAAGGTAGCTGGGAAGTTGCAGAAGCAAGGACAAACAGTAATAGGCAACTTCTTGAATATTTGGTTTACTTAGCAATGGAAGCTGGTTACTCAGAGAAGGTTGATGAGCTATGTGATCGATACTCGCTTGAAGGTTTCTTGAATGGAAAAGTACCCAAGGCAAGTCTTCTCGAAAATCGCTATTTGCATCTCAATCAGTTGGTTATAGAGGGTGTTGTTTGGGTTGATGAAGCTGATGCCTTGCATAATGCTACCTCTCACTTTGAGGGATGCAAAGTTGTGGGTCTTGATTGCGAATGGAAACCGAACTACGTAAAAGGCAGCAAACCGAGCAAGGTTTCCATCATGCAAATTGCTTCTGAAAAAAAAGTTTTCGTCGTGGACTTGATCAAGTTATTCGCTGACGCTCCTGATGTCCTGGATGACTGCTTAACTCGCATTCTGCAATCTCCTAGGATTCTGAAACTCGGCTATAATTTTCAGTGTGATATGAAGCAGTTGGCTCATTCGTACGGAGAGTTAGAGTGTTTTAAACACTACGAAATGTTACTGGACGTCCAGAATGTTTTCAAAGAACCTAGCGGCGGTCTCTCTGGGCTTGCAAAGAAAATACTGGGAGTAGGTTTGAACAAGACAAGAAGGAATAGCAATTGGGAGCAACGACCTTTGACGCCCAATCAGCTGGAGTATGCAGCTCTTGATGCAGCTGTGCTGATTCACATATTTCGCCACGTTCGCAATCATTCCCAGACTGCCGAGGGAGGAAAACTCGAGTGGAAATCCTGCATCGTTTCCCACATGGATAACAAGAAGGCGAAGAAGGGGAGTCGAAAGGGTTCATCAGAAGCTGAGACAATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_pol_A_exo1

Protein Analysis

570

Amino Acids

64.78

Weight (kDa)

8.31

Isoelectric Point (pI)

33.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_pol_A_exo1 PF01612 356 - 530 1.4e-24 3'-5' exonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1407
AccI GTMKAC 1 cut(s) 120
AccII CGCG 1 cut(s) 587
AciI CCGC 5 cut(s) 585, 587, 616, 1431, 1434
AclWI GGATC 1 cut(s) 209
AcoI YGGCCR 1 cut(s) 625
AcsI RAATTY 1 cut(s) 794
AcyI GRCGYC 2 cut(s) 1404, 1520
AfaI GTAC 5 cut(s) 204, 731, 818, 1002, 1361
AfiI CCNNNNNNNGG 3 cut(s) 224, 334, 1433
AflIII ACRYGT 1 cut(s) 152
AgsI TTSAA 9 cut(s) 130, 185, 449, 667, 905, 980, 991, 1420, 1477
AjnI CCWGG 3 cut(s) 189, 223, 1266
Alw21I GWGCWC 3 cut(s) 181, 606, 830
Alw26I GTCTC 3 cut(s) 573, 1442, 1694
AlwI GGATC 1 cut(s) 209
AlwNI CAGNNNCTG 1 cut(s) 1697
Ama87I CYCGRG 2 cut(s) 346, 1619
AoxI GGCC 1 cut(s) 625
ApeKI GCWGC 5 cut(s) 377, 671, 1170, 1541, 1553
ApoI RAATTY 1 cut(s) 794
Asp700I GAANNNNTTC 2 cut(s) 1415, 1684
AspA2I CCTAGG 1 cut(s) 1301
AspS9I GGNCC 1 cut(s) 227
AsuC2I CCSGG 1 cut(s) 725
AvaI CYCGRG 2 cut(s) 346, 1619
AvaII GGWCC 1 cut(s) 227
AvrII CCTAGG 1 cut(s) 1301
BalI TGGCCA 1 cut(s) 627
BanII GRGCYC 2 cut(s) 181, 410
BbsI GAAGAC 2 cut(s) 308, 1007
Bbv12I GWGCWC 3 cut(s) 181, 606, 830
BbvI GCAGC 5 cut(s) 364, 683, 1182, 1553, 1565
BccI CCATC 1 cut(s) 1199
BciT130I CCWGG 3 cut(s) 191, 225, 1268
BclI TGATCA 1 cut(s) 1236
BcnI CCSGG 1 cut(s) 725
BcoDI GTCTC 3 cut(s) 573, 1442, 1694
BfaI CTAG 4 cut(s) 200, 842, 1302, 1428
BglI GCCNNNNNGGC 1 cut(s) 624
BisI GCNGC 6 cut(s) 378, 672, 1171, 1432, 1542, 1554
BlnI CCTAGG 1 cut(s) 1301
BlsI GCNGC 6 cut(s) 379, 673, 1172, 1433, 1543, 1555
BmcAI AGTACT 2 cut(s) 204, 731
Bme1390I CCNGG 4 cut(s) 191, 225, 725, 1268
Bme18I GGWCC 1 cut(s) 227
BmeT110I CYCGRG 2 cut(s) 346, 1619
BmgT120I GGNCC 1 cut(s) 227
BmrFI CCNGG 4 cut(s) 191, 225, 725, 1268
BmrI ACTGGG 2 cut(s) 376, 1472
BmsI GCATC 5 cut(s) 1045, 1075, 1109, 1540, 1644
BmuI ACTGGG 2 cut(s) 376, 1472
BpiI GAAGAC 2 cut(s) 308, 1007
BplI GAGNNNNNCTC 4 cut(s) 593, 625, 1602, 1634
BpmI CTGGAG 1 cut(s) 1553
BpuMI CCSGG 1 cut(s) 725
Bsa29I ATCGAT 1 cut(s) 967
BsaAI YACGTR 1 cut(s) 1162
BsaBI GATNNNNATC 1 cut(s) 807
BsaHI GRCGYC 2 cut(s) 1404, 1520
BsaI GGTCTC 1 cut(s) 1442
BsaJI CCNNGG 5 cut(s) 145, 585, 1005, 1301, 1605
Bsc4I CCNNNNNNNGG 3 cut(s) 224, 334, 1433
Bse1I ACTGG 4 cut(s) 382, 619, 1404, 1467
Bse3DI GCAATG 2 cut(s) 217, 930
Bse8I GATNNNNATC 1 cut(s) 807
BseBI CCWGG 3 cut(s) 191, 225, 1268
BseCI ATCGAT 1 cut(s) 967
BseDI CCNNGG 5 cut(s) 145, 585, 1005, 1301, 1605
BseGI GGATG 2 cut(s) 1124, 1276
BseJI GATNNNNATC 1 cut(s) 807
BseLI CCNNNNNNNGG 3 cut(s) 224, 334, 1433
BseMI GCAATG 2 cut(s) 217, 930
BseMII CTCAG 2 cut(s) 954, 1689
BseNI ACTGG 4 cut(s) 382, 619, 1404, 1467
BseXI GCAGC 5 cut(s) 364, 683, 1182, 1553, 1565
BseYI CCCAGC 1 cut(s) 858
Bsh1236I CGCG 1 cut(s) 587
BshFI GGCC 1 cut(s) 627
BshVI ATCGAT 1 cut(s) 967
BsiHKAI GWGCWC 3 cut(s) 181, 606, 830
BsiHKCI CYCGRG 2 cut(s) 346, 1619
BsiSI CCGG 1 cut(s) 724
BsiWI CGTACG 1 cut(s) 1359
BslFI GGGAC 1 cut(s) 365
BslI CCNNNNNNNGG 3 cut(s) 224, 334, 1433
BsmAI GTCTC 3 cut(s) 573, 1442, 1694
BsmFI GGGAC 1 cut(s) 365
BsmI GAATGC 2 cut(s) 502, 1287
BsnI GGCC 1 cut(s) 627
Bso31I GGTCTC 1 cut(s) 1442
BsoBI CYCGRG 2 cut(s) 346, 1619
Bsp1286I GDGCHC 4 cut(s) 181, 410, 606, 830
Bsp143I GATC 3 cut(s) 214, 964, 1236
Bsp19I CCATGG 1 cut(s) 145
BspACI CCGC 5 cut(s) 585, 587, 616, 1431, 1434
BspANI GGCC 1 cut(s) 627
BspCNI CTCAG 2 cut(s) 953, 1690
BspDI ATCGAT 1 cut(s) 967
BspFNI CGCG 1 cut(s) 587
BspPI GGATC 1 cut(s) 209
BspQI GCTCTTC 1 cut(s) 186
BspTNI GGTCTC 1 cut(s) 1442
BsrDI GCAATG 2 cut(s) 217, 930
BsrI ACTGG 4 cut(s) 382, 619, 1404, 1467
BssECI CCNNGG 5 cut(s) 145, 585, 1005, 1301, 1605
BssMI GATC 3 cut(s) 214, 964, 1236
BssNAI GTATAC 1 cut(s) 121
BssNI GRCGYC 2 cut(s) 1404, 1520
BssT1I CCWWGG 3 cut(s) 145, 1005, 1301
Bst1107I GTATAC 1 cut(s) 121
Bst2UI CCWGG 3 cut(s) 191, 225, 1268
Bst4CI ACNGT 2 cut(s) 284, 887
Bst6I CTCTTC 1 cut(s) 186
BstACI GRCGYC 2 cut(s) 1404, 1520
BstBAI YACGTR 1 cut(s) 1162
BstC8I GCNNGC 3 cut(s) 74, 78, 1449
BstDEI CTNAG 4 cut(s) 165, 919, 940, 1698
BstDSI CCRYGG 2 cut(s) 145, 585
BstENI CCTNNNNNAGG 1 cut(s) 332
BstF5I GGATG 2 cut(s) 1124, 1276
BstFNI CGCG 1 cut(s) 587
BstKTI GATC 3 cut(s) 217, 967, 1239
BstMAI GTCTC 3 cut(s) 573, 1442, 1694
BstMBI GATC 3 cut(s) 214, 964, 1236
BstMWI GCNNNNNNNGC 7 cut(s) 624, 929, 1179, 1284, 1528, 1550, 1584
BstNI CCWGG 3 cut(s) 191, 225, 1268
BstNSI RCATGY 4 cut(s) 80, 156, 345, 748
BstSCI CCNGG 4 cut(s) 189, 223, 723, 1266
BstSNI TACGTA 1 cut(s) 1162
BstUI CGCG 1 cut(s) 587
BstV1I GCAGC 5 cut(s) 364, 683, 1182, 1553, 1565
BstV2I GAAGAC 2 cut(s) 308, 1007
BstXI CCANNNNNNTGG 1 cut(s) 1531
BstZ17I GTATAC 1 cut(s) 121
Bsu15I ATCGAT 1 cut(s) 967
BsuRI GGCC 1 cut(s) 627
BsuTUI ATCGAT 1 cut(s) 967
BtgI CCRYGG 2 cut(s) 145, 585
BtsCI GGATG 2 cut(s) 1124, 1276
BtsIMutI CAGTG 2 cut(s) 254, 1337
Cac8I GCNNGC 3 cut(s) 74, 78, 1449
CaiI CAGNNNCTG 1 cut(s) 1697
Cfr13I GGNCC 1 cut(s) 227
Cfr42I CCGCGG 1 cut(s) 588
ClaI ATCGAT 1 cut(s) 967
CseI GACGC 3 cut(s) 103, 1262, 1528
CsiI ACCWGGT 1 cut(s) 189
Csp6I GTAC 5 cut(s) 203, 730, 817, 1001, 1360
CviQI GTAC 5 cut(s) 203, 730, 817, 1001, 1360
DdeI CTNAG 4 cut(s) 165, 919, 940, 1698
DpnI GATC 3 cut(s) 216, 966, 1238
DpnII GATC 3 cut(s) 214, 964, 1236
DraI TTTAAA 2 cut(s) 834, 1381
EaeI YGGCCR 1 cut(s) 625
Eam1104I CTCTTC 1 cut(s) 186
EarI CTCTTC 1 cut(s) 186
EciI GGCGGA 1 cut(s) 605
Ecl136II GAGCTC 1 cut(s) 179
Eco105I TACGTA 1 cut(s) 1162
Eco130I CCWWGG 3 cut(s) 145, 1005, 1301
Eco24I GRGCYC 2 cut(s) 181, 410
Eco31I GGTCTC 1 cut(s) 1442
Eco47I GGWCC 1 cut(s) 227
Eco53kI GAGCTC 1 cut(s) 179
Eco88I CYCGRG 2 cut(s) 346, 1619
EcoICRI GAGCTC 1 cut(s) 179
EcoNI CCTNNNNNAGG 1 cut(s) 332
EcoRII CCWGG 3 cut(s) 189, 223, 1266
EcoT14I CCWWGG 3 cut(s) 145, 1005, 1301
EcoT38I GRGCYC 2 cut(s) 181, 410
ErhI CCWWGG 3 cut(s) 145, 1005, 1301
FaqI GGGAC 1 cut(s) 365
FbaI TGATCA 1 cut(s) 1236
FblI GTMKAC 1 cut(s) 120
Fnu4HI GCNGC 6 cut(s) 378, 672, 1171, 1432, 1542, 1554
FokI GGATG 2 cut(s) 1131, 1283
FriOI GRGCYC 2 cut(s) 181, 410
Fsp4HI GCNGC 6 cut(s) 378, 672, 1171, 1432, 1542, 1554
FspBI CTAG 4 cut(s) 200, 842, 1302, 1428
GluI GCNGC 6 cut(s) 378, 672, 1171, 1432, 1542, 1554
GsaI CCCAGC 1 cut(s) 862
GsuI CTGGAG 1 cut(s) 1553
HaeIII GGCC 1 cut(s) 627
HapII CCGG 1 cut(s) 724
HgaI GACGC 3 cut(s) 103, 1262, 1528
Hin1I GRCGYC 2 cut(s) 1404, 1520
HincII GTYRAC 2 cut(s) 22, 415
HindII GTYRAC 2 cut(s) 22, 415
HinfI GANTC 5 cut(s) 353, 566, 1306, 1564, 1675
HpaII CCGG 1 cut(s) 724
Hpy166II GTNNAC 6 cut(s) 22, 121, 415, 472, 916, 1231
Hpy188I TCNGA 8 cut(s) 57, 88, 176, 788, 943, 1210, 1311, 1693
Hpy8I GTNNAC 6 cut(s) 22, 121, 415, 472, 916, 1231
Hpy99I CGWCG 1 cut(s) 1229
HpyAV CCTTC 7 cut(s) 281, 650, 940, 974, 1482, 1654, 1663
HpyCH4III ACNGT 2 cut(s) 284, 887
HpyCH4IV ACGT 3 cut(s) 1161, 1404, 1581
HpyF10VI GCNNNNNNNGC 7 cut(s) 624, 929, 1179, 1284, 1528, 1550, 1584
HpyF3I CTNAG 4 cut(s) 165, 919, 940, 1698
HpySE526I ACGT 3 cut(s) 1161, 1404, 1581
Hsp92I GRCGYC 2 cut(s) 1404, 1520
Ksp22I TGATCA 1 cut(s) 1236
KspI CCGCGG 1 cut(s) 588
Kzo9I GATC 3 cut(s) 214, 964, 1236
LguI GCTCTTC 1 cut(s) 186
LmnI GCTCC 2 cut(s) 1261, 1504
Lsp1109I GCAGC 5 cut(s) 364, 683, 1182, 1553, 1565
LweI GCATC 5 cut(s) 1045, 1075, 1109, 1540, 1644
MabI ACCWGGT 1 cut(s) 189
MaeI CTAG 4 cut(s) 200, 842, 1302, 1428
MaeII ACGT 3 cut(s) 1161, 1404, 1581
MaeIII GTNAC 3 cut(s) 589, 935, 1395
MalI GATC 3 cut(s) 216, 966, 1238
MboI GATC 3 cut(s) 214, 964, 1236
MboII GAAGA 5 cut(s) 173, 308, 451, 1007, 1678
MfeI CAATTG 2 cut(s) 662, 1498
MhlI GDGCHC 4 cut(s) 181, 410, 606, 830
MlsI TGGCCA 1 cut(s) 627
MluCI AATT 7 cut(s) 432, 508, 662, 794, 1200, 1324, 1498
MluNI TGGCCA 1 cut(s) 627
MlyI GAGTC 3 cut(s) 347, 575, 1684
MmeI TCCRAC 1 cut(s) 811
Mox20I TGGCCA 1 cut(s) 627
MroXI GAANNNNTTC 2 cut(s) 1415, 1684
MscI TGGCCA 1 cut(s) 627
MseI TTAA 4 cut(s) 51, 833, 1280, 1380
Msp20I TGGCCA 1 cut(s) 627
MspA1I CMGCKG 3 cut(s) 587, 1531, 1556
MspI CCGG 1 cut(s) 724
MspR9I CCNGG 4 cut(s) 191, 225, 725, 1268
MunI CAATTG 2 cut(s) 662, 1498
Mva1269I GAATGC 2 cut(s) 502, 1287
MvaI CCWGG 3 cut(s) 191, 225, 1268
MvnI CGCG 1 cut(s) 587
MwoI GCNNNNNNNGC 7 cut(s) 624, 929, 1179, 1284, 1528, 1550, 1584
NciI CCSGG 1 cut(s) 725
NcoI CCATGG 1 cut(s) 145
NdeII GATC 3 cut(s) 214, 964, 1236
NmeAIII GCCGAG 2 cut(s) 1296, 1630
NspI RCATGY 4 cut(s) 80, 156, 345, 748
PaeI GCATGC 1 cut(s) 80
PaeR7I CTCGAG 1 cut(s) 1619
PciI ACATGT 1 cut(s) 152
PciSI GCTCTTC 1 cut(s) 186
PctI GAATGC 2 cut(s) 502, 1287
PdmI GAANNNNTTC 2 cut(s) 1415, 1684
PfeI GAWTC 2 cut(s) 1306, 1564
Pfl23II CGTACG 1 cut(s) 1359
PfoI TCCNGGA 2 cut(s) 723, 1266
PkrI GCNGC 6 cut(s) 379, 673, 1172, 1433, 1543, 1555
PleI GAGTC 3 cut(s) 347, 574, 1683
PpsI GAGTC 3 cut(s) 347, 574, 1683
Ppu21I YACGTR 1 cut(s) 1162
PscI ACATGT 1 cut(s) 152
Psp124BI GAGCTC 1 cut(s) 181
Psp6I CCWGG 3 cut(s) 189, 223, 1266
PspFI CCCAGC 1 cut(s) 858
PspGI CCWGG 3 cut(s) 189, 223, 1266
PspLI CGTACG 1 cut(s) 1359
PspPI GGNCC 1 cut(s) 227
PspXI VCTCGAGB 1 cut(s) 1619
PstNI CAGNNNCTG 1 cut(s) 1697
PvuII CAGCTG 2 cut(s) 1531, 1556
RsaI GTAC 5 cut(s) 204, 731, 818, 1002, 1361
RsaNI GTAC 5 cut(s) 203, 730, 817, 1001, 1360
SacI GAGCTC 1 cut(s) 181
SacII CCGCGG 1 cut(s) 588
SapI GCTCTTC 1 cut(s) 186
SaqAI TTAA 4 cut(s) 51, 833, 1280, 1380
SatI GCNGC 6 cut(s) 378, 672, 1171, 1432, 1542, 1554
Sau3AI GATC 3 cut(s) 214, 964, 1236
Sau96I GGNCC 1 cut(s) 227
ScaI AGTACT 2 cut(s) 204, 731
SchI GAGTC 3 cut(s) 347, 575, 1684
ScrFI CCNGG 4 cut(s) 191, 225, 725, 1268
SduI GDGCHC 4 cut(s) 181, 410, 606, 830
SexAI ACCWGGT 1 cut(s) 189
SfaNI GCATC 5 cut(s) 1045, 1075, 1109, 1540, 1644
Sfr274I CTCGAG 1 cut(s) 1619
Sfr303I CCGCGG 1 cut(s) 588
SgrBI CCGCGG 1 cut(s) 588
SinI GGWCC 1 cut(s) 227
SlaI CTCGAG 1 cut(s) 1619
SmlI CTYRAG 1 cut(s) 1619
SmoI CTYRAG 1 cut(s) 1619
SnaBI TACGTA 1 cut(s) 1162
SphI GCATGC 1 cut(s) 80
Sse9I AATT 7 cut(s) 432, 508, 662, 794, 1200, 1324, 1498
SsiI CCGC 5 cut(s) 585, 587, 616, 1431, 1434
SspI AATATT 1 cut(s) 908
SspMI CTAG 4 cut(s) 200, 842, 1302, 1428
SstI GAGCTC 1 cut(s) 181
StyD4I CCNGG 4 cut(s) 189, 223, 723, 1266
StyI CCWWGG 3 cut(s) 145, 1005, 1301
TaaI ACNGT 2 cut(s) 284, 887
TaiI ACGT 3 cut(s) 1164, 1407, 1584
TaqI TCGA 5 cut(s) 393, 967, 1020, 1620, 1678
TasI AATT 7 cut(s) 432, 508, 662, 794, 1200, 1324, 1498
TatI WGTACW 3 cut(s) 202, 729, 816
TauI GCSGC 1 cut(s) 1434
TfiI GAWTC 2 cut(s) 1306, 1564
Tru1I TTAA 4 cut(s) 51, 833, 1280, 1380
Tru9I TTAA 4 cut(s) 51, 833, 1280, 1380
TscAI CASTG 2 cut(s) 254, 1337
TseI GCWGC 5 cut(s) 377, 671, 1170, 1541, 1553
TspDTI ATGAA 5 cut(s) 682, 807, 1092, 1355, 1677
TspGWI ACGGA 2 cut(s) 96, 1377
TspRI CASTG 2 cut(s) 254, 1337
VpaK11BI GGWCC 1 cut(s) 227
XagI CCTNNNNNAGG 1 cut(s) 332
XapI RAATTY 1 cut(s) 794
XceI RCATGY 4 cut(s) 80, 156, 345, 748
XhoI CTCGAG 1 cut(s) 1619
XmaJI CCTAGG 1 cut(s) 1301
XmiI GTMKAC 1 cut(s) 120
XmnI GAANNNNTTC 2 cut(s) 1415, 1684
XspI CTAG 4 cut(s) 200, 842, 1302, 1428
ZraI GACGTC 1 cut(s) 1405
ZrmI AGTACT 2 cut(s) 204, 731
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.