Rroxscaffold_6G00422480

3'-5' exonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0003918
Physical Location & Seq
Reverse (-)
43355428 .. 43359193
3766 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00422480.1

Sequence Viewer

Length: 1722 bp
ATGGGTTTGGAGGAAAGGGCTGCCACATTTAACAGCCAGAACGGCAAATGTAATCAGACATTCACTATTTGCAAGCATGGATTTACTGACCTATCGCACATTTCTCCATTGGTGTTCATATACCTCTTGAAAGAATGTTACTTAATTGGCACATGCAAGGCAACTAAGAAGTTCCGAGCTCTTCAAGAACAGGTCCATCTAGTGCTACGCAATGATCCCCAACCTGGACCAGCTACTTTTGTTATTCAGTGCCTCTATGTCTTGCCTATATTTGACTTATACTCTGAAGGGTTCAGTCATTTAATTATATCTGCTCTTCGTCGTTTCCTAAAAGAAGAAACTTCCGCGGGGGACTCATTGGAAGCAAAAGACATTGCTGCCCAGTTATTTCTAGATATTGTTGGGGGCTCTGTCAACCATGATGAGAGGATAGTTGAGAAGATGCTTGAAGTTTTTGATGTTAAGTTAGAGAATATTGAAAAAGTTTTGTGCCCAAATGCAATAAATGATGACACTTTTAGCATGGCAACAAAATTTGTTGAGGATTATATATATCAATTGATAGAGTCTCAAACATACATGACTGCGGTTACCCTATTGGCGCACTTCTCTATCCGCCAGTCTGGTGAATCTTTTCTGCTTTGTATGATAGAAAGCCAACAATTGAAAGCAGCAGAGAAGTGGGCAACATTCATCGGAAAGCCAATGTTATGTGTTCTGGTCCAAGACTCATTAAAGAAACTAGCAGAAAAAGGATGCTGGGATGTTGCAGAAGCAAAGACAAAGAGTAATAGACAACTCCTTGAATACCTGGTTTATTTAGCCATGGAAGCTGGTTACTCAGAAAAGGTTGATGAACTATGTGATCGATATTCACTTAAAGGTTTCTTGAATGCCAAAGGTGTCAATCTTAAAAGCCTATGTGTTTCCCTTAATGCTGCTAGTTGTTTTGCTGCTTCTAGAAAGAAATACACAATAGGAATCTTGCCTCAGCCAAGCCTTCTCCACAGTCGCTATTTGCATCTGGATGAGTTAGTTGTAGAAGGTGTCATTTGGGTTGATGAAGTTAATGCTTTGCGTAAGGCAACCTGTCATCTTGAGGAATGCAAAGTTGTTGGTCTTGATTGTGAATGGAAACCGAATTATGTAAAAGGTAGCAAACCAAACAAGGTTTCTATTATGCAAATTGCTTCTGATAAGATGGTTTTCATACTTGACTTGATCAAATTATATGAAGATGTGCCTGATGTTCTGGATGACTGCTTAACTCGTATTGTACAGTCTCCTAGGATCCTAAAACTTGGCTATAATTTTCAATGTGATATGAGGCAGCTGGCTCAGTCATATGAAGAGTTAGAGTGTTTCAAGCACTATCAGATGCTACTGGACATTCAGAATGTGTTCAAAGAACCTCGGGGTGGTCTATCCGGGCTTGCAAAGAAAATACTTGGAGTTGGTTTGAACAAGACAAGAAGGAACAGCAATTGGGAGCAACGGCCTCTAACACCCAATCAGCTGGAATATGCAGCTCTTGATGCAGCTGTACTGGTTCACATATTTGGCCACGTTCGCGGTCATTCTCAGCCTGGTGATGGAGAGGGACATGGAAAACTCGAGTGGAAATCCTGCATTGTTTCCGACATGAATAACATCAAGAAGTCCAAGAAGGGAAGTCGAAAGGAGTCTTCTGAAGCTCAGACAAAGGAGCATTTAGCAATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_pol_A_exo1

Protein Analysis

573

Amino Acids

64.69

Weight (kDa)

6.74

Isoelectric Point (pI)

39.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_pol_A_exo1 PF01612 351 - 522 6.9e-22 3'-5' exonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 347, 1572
AciI CCGC 5 cut(s) 345, 347, 587, 616, 1572
AclWI GGATC 3 cut(s) 209, 1285, 1298
AcoI YGGCCR 1 cut(s) 1561
AcsI RAATTY 1 cut(s) 533
AcuI CTGAAG 2 cut(s) 306, 1710
AfaI GTAC 2 cut(s) 1278, 1545
AfiI CCNNNNNNNGG 3 cut(s) 224, 1418, 1592
AjnI CCWGG 3 cut(s) 223, 810, 1585
AjuI GAANNNNNNNTTGG 2 cut(s) 1156, 1188
AluBI AGCT 8 cut(s) 179, 233, 833, 1333, 1516, 1529, 1541, 1694
AluI AGCT 8 cut(s) 179, 233, 833, 1333, 1516, 1529, 1541, 1694
Alw21I GWGCWC 1 cut(s) 181
Alw26I GTCTC 2 cut(s) 573, 1287
AlwI GGATC 3 cut(s) 209, 1285, 1298
Ama87I CYCGRG 2 cut(s) 1413, 1613
AoxI GGCC 2 cut(s) 1496, 1561
ApeKI GCWGC 8 cut(s) 20, 377, 671, 938, 953, 1330, 1526, 1538
ApoI RAATTY 1 cut(s) 533
Asp700I GAANNNNTTC 2 cut(s) 633, 1400
AspA2I CCTAGG 1 cut(s) 1286
AspLEI GCGC 1 cut(s) 604
AspS9I GGNCC 3 cut(s) 193, 227, 721
AsuC2I CCSGG 1 cut(s) 1429
AsuHPI GGTGA 2 cut(s) 638, 1601
AvaI CYCGRG 2 cut(s) 1413, 1613
AvaII GGWCC 3 cut(s) 193, 227, 721
AvrII CCTAGG 1 cut(s) 1286
BaeGI GKGCMC 1 cut(s) 494
BalI TGGCCA 1 cut(s) 1563
BamHI GGATCC 1 cut(s) 1290
BanII GRGCYC 2 cut(s) 181, 410
BbsI GAAGAC 1 cut(s) 1677
Bbv12I GWGCWC 1 cut(s) 181
BbvCI CCTCAGC 1 cut(s) 990
BbvI GCAGC 8 cut(s) 7, 364, 683, 925, 940, 1342, 1538, 1550
BccI CCATC 3 cut(s) 204, 1195, 1586
BceAI ACGGC 2 cut(s) 58, 1511
BciT130I CCWGG 3 cut(s) 225, 812, 1587
BclI TGATCA 1 cut(s) 1221
BcnI CCSGG 1 cut(s) 1429
BcoDI GTCTC 2 cut(s) 573, 1287
BfaI CTAG 6 cut(s) 200, 392, 743, 942, 960, 1287
BglI GCCNNNNNGGC 1 cut(s) 42
BisI GCNGC 8 cut(s) 21, 378, 672, 939, 954, 1331, 1527, 1539
BlnI CCTAGG 1 cut(s) 1286
BlsI GCNGC 8 cut(s) 22, 379, 673, 940, 955, 1332, 1528, 1540
Bme1390I CCNGG 4 cut(s) 225, 812, 1429, 1587
Bme18I GGWCC 3 cut(s) 193, 227, 721
BmeT110I CYCGRG 2 cut(s) 1413, 1613
BmgT120I GGNCC 3 cut(s) 193, 227, 721
BmiI GGNNCC 1 cut(s) 1292
BmrFI CCNGG 4 cut(s) 225, 812, 1429, 1587
BmrI ACTGGG 1 cut(s) 376
BmsI GCATC 5 cut(s) 432, 746, 1030, 1368, 1525
BmuI ACTGGG 1 cut(s) 376
BpiI GAAGAC 1 cut(s) 1677
Bpu10I CCTNAGC 1 cut(s) 990
BpuEI CTTGAG 1 cut(s) 1118
BpuMI CCSGG 1 cut(s) 1429
Bsa29I ATCGAT 1 cut(s) 868
BsaJI CCNNGG 4 cut(s) 345, 825, 1286, 1412
Bsc4I CCNNNNNNNGG 3 cut(s) 224, 1418, 1592
Bse1I ACTGG 4 cut(s) 382, 619, 1389, 1551
Bse3DI GCAATG 2 cut(s) 217, 372
BseBI CCWGG 3 cut(s) 225, 812, 1587
BseCI ATCGAT 1 cut(s) 868
BseDI CCNNGG 4 cut(s) 345, 825, 1286, 1412
BseGI GGATG 4 cut(s) 761, 769, 1033, 1261
BseLI CCNNNNNNNGG 3 cut(s) 224, 1418, 1592
BseMI GCAATG 2 cut(s) 217, 372
BseMII CTCAG 5 cut(s) 855, 1004, 1352, 1595, 1709
BseNI ACTGG 4 cut(s) 382, 619, 1389, 1551
BseSI GKGCMC 1 cut(s) 494
BseXI GCAGC 8 cut(s) 7, 364, 683, 925, 940, 1342, 1538, 1550
BseYI CCCAGC 1 cut(s) 759
Bsh1236I CGCG 2 cut(s) 347, 1572
BshFI GGCC 2 cut(s) 1498, 1563
BshVI ATCGAT 1 cut(s) 868
BsiHKAI GWGCWC 1 cut(s) 181
BsiHKCI CYCGRG 2 cut(s) 1413, 1613
BsiSI CCGG 1 cut(s) 1428
BslFI GGGAC 2 cut(s) 365, 1614
BslI CCNNNNNNNGG 3 cut(s) 224, 1418, 1592
BsmAI GTCTC 2 cut(s) 573, 1287
BsmFI GGGAC 2 cut(s) 365, 1614
BsmI GAATGC 2 cut(s) 898, 1109
BsnI GGCC 2 cut(s) 1498, 1563
BsoBI CYCGRG 2 cut(s) 1413, 1613
Bsp1286I GDGCHC 3 cut(s) 181, 410, 494
Bsp1407I TGTACA 1 cut(s) 1276
Bsp143I GATC 4 cut(s) 214, 865, 1221, 1290
Bsp19I CCATGG 1 cut(s) 825
BspACI CCGC 5 cut(s) 345, 347, 587, 616, 1572
BspANI GGCC 2 cut(s) 1498, 1563
BspCNI CTCAG 5 cut(s) 854, 1003, 1351, 1594, 1708
BspDI ATCGAT 1 cut(s) 868
BspFNI CGCG 2 cut(s) 347, 1572
BspLI GGNNCC 1 cut(s) 1292
BspPI GGATC 3 cut(s) 209, 1285, 1298
BspQI GCTCTTC 2 cut(s) 186, 321
BsrDI GCAATG 2 cut(s) 217, 372
BsrGI TGTACA 1 cut(s) 1276
BsrI ACTGG 4 cut(s) 382, 619, 1389, 1551
BssECI CCNNGG 4 cut(s) 345, 825, 1286, 1412
BssMI GATC 4 cut(s) 214, 865, 1221, 1290
BssT1I CCWWGG 2 cut(s) 825, 1286
Bst2UI CCWGG 3 cut(s) 225, 812, 1587
Bst4CI ACNGT 2 cut(s) 1010, 1281
Bst6I CTCTTC 3 cut(s) 186, 321, 1344
BstAUI TGTACA 1 cut(s) 1276
BstC8I GCNNGC 3 cut(s) 74, 1335, 1434
BstDEI CTNAG 6 cut(s) 165, 841, 990, 1338, 1581, 1695
BstDSI CCRYGG 2 cut(s) 345, 825
BstEII GGTNACC 1 cut(s) 589
BstF5I GGATG 4 cut(s) 761, 769, 1033, 1261
BstFNI CGCG 2 cut(s) 347, 1572
BstHHI GCGC 1 cut(s) 604
BstKTI GATC 4 cut(s) 217, 868, 1224, 1293
BstMAI GTCTC 2 cut(s) 573, 1287
BstMBI GATC 4 cut(s) 214, 865, 1221, 1290
BstMWI GCNNNNNNNGC 4 cut(s) 42, 830, 1535, 1569
BstNI CCWGG 3 cut(s) 225, 812, 1587
BstNSI RCATGY 1 cut(s) 156
BstPI GGTNACC 1 cut(s) 589
BstSCI CCNGG 4 cut(s) 223, 810, 1427, 1585
BstSLI GKGCMC 1 cut(s) 494
BstUI CGCG 2 cut(s) 347, 1572
BstV1I GCAGC 8 cut(s) 7, 364, 683, 925, 940, 1342, 1538, 1550
BstV2I GAAGAC 1 cut(s) 1677
BstX2I RGATCY 1 cut(s) 1290
BstXI CCANNNNNNTGG 1 cut(s) 1516
BstYI RGATCY 1 cut(s) 1290
Bsu15I ATCGAT 1 cut(s) 868
BsuRI GGCC 2 cut(s) 1498, 1563
BsuTUI ATCGAT 1 cut(s) 868
BtgI CCRYGG 2 cut(s) 345, 825
BtsCI GGATG 4 cut(s) 761, 769, 1033, 1261
BtsIMutI CAGTG 1 cut(s) 254
Cac8I GCNNGC 3 cut(s) 74, 1335, 1434
CfoI GCGC 1 cut(s) 604
Cfr13I GGNCC 3 cut(s) 193, 227, 721
Cfr42I CCGCGG 1 cut(s) 348
ClaI ATCGAT 1 cut(s) 868
CsiI ACCWGGT 1 cut(s) 810
Csp6I GTAC 2 cut(s) 1277, 1544
CviAII CATG 8 cut(s) 77, 153, 419, 523, 580, 826, 1604, 1642
CviQI GTAC 2 cut(s) 1277, 1544
DdeI CTNAG 6 cut(s) 165, 841, 990, 1338, 1581, 1695
DpnI GATC 4 cut(s) 216, 867, 1223, 1292
DpnII GATC 4 cut(s) 214, 865, 1221, 1290
EaeI YGGCCR 1 cut(s) 1561
Eam1104I CTCTTC 3 cut(s) 186, 321, 1344
EarI CTCTTC 3 cut(s) 186, 321, 1344
EciI GGCGGA 1 cut(s) 605
Ecl136II GAGCTC 1 cut(s) 179
Eco130I CCWWGG 2 cut(s) 825, 1286
Eco24I GRGCYC 2 cut(s) 181, 410
Eco47I GGWCC 3 cut(s) 193, 227, 721
Eco53kI GAGCTC 1 cut(s) 179
Eco57I CTGAAG 2 cut(s) 306, 1710
Eco88I CYCGRG 2 cut(s) 1413, 1613
Eco91I GGTNACC 1 cut(s) 589
EcoICRI GAGCTC 1 cut(s) 179
EcoO65I GGTNACC 1 cut(s) 589
EcoRII CCWGG 3 cut(s) 223, 810, 1585
EcoT14I CCWWGG 2 cut(s) 825, 1286
EcoT38I GRGCYC 2 cut(s) 181, 410
ErhI CCWWGG 2 cut(s) 825, 1286
FaeI CATG 8 cut(s) 80, 156, 422, 526, 583, 829, 1607, 1645
FaqI GGGAC 2 cut(s) 365, 1614
FatI CATG 8 cut(s) 76, 152, 418, 522, 579, 825, 1603, 1641
FauI CCCGC 1 cut(s) 340
FauNDI CATATG 1 cut(s) 1345
FbaI TGATCA 1 cut(s) 1221
Fnu4HI GCNGC 8 cut(s) 21, 378, 672, 939, 954, 1331, 1527, 1539
FokI GGATG 4 cut(s) 768, 776, 1040, 1268
FriOI GRGCYC 2 cut(s) 181, 410
Fsp4HI GCNGC 8 cut(s) 21, 378, 672, 939, 954, 1331, 1527, 1539
FspBI CTAG 6 cut(s) 200, 392, 743, 942, 960, 1287
GlaI GCGC 1 cut(s) 603
GluI GCNGC 8 cut(s) 21, 378, 672, 939, 954, 1331, 1527, 1539
GsaI CCCAGC 1 cut(s) 763
HaeIII GGCC 2 cut(s) 1498, 1563
HapII CCGG 1 cut(s) 1428
HhaI GCGC 1 cut(s) 604
Hin1II CATG 8 cut(s) 80, 156, 422, 526, 583, 829, 1607, 1645
Hin6I GCGC 1 cut(s) 602
HinP1I GCGC 1 cut(s) 602
HincII GTYRAC 1 cut(s) 415
HindII GTYRAC 1 cut(s) 415
HinfI GANTC 6 cut(s) 353, 566, 629, 728, 981, 1682
HpaII CCGG 1 cut(s) 1428
HphI GGTGA 2 cut(s) 638, 1601
Hpy166II GTNNAC 2 cut(s) 415, 1552
Hpy8I GTNNAC 2 cut(s) 415, 1552
Hpy99I CGWCG 1 cut(s) 324
HpyAV CCTTC 5 cut(s) 281, 1010, 1037, 1467, 1660
HpyCH4III ACNGT 2 cut(s) 1010, 1281
HpyCH4IV ACGT 1 cut(s) 1566
HpyF10VI GCNNNNNNNGC 4 cut(s) 42, 830, 1535, 1569
HpyF3I CTNAG 6 cut(s) 165, 841, 990, 1338, 1581, 1695
HpySE526I ACGT 1 cut(s) 1566
Hsp92II CATG 8 cut(s) 80, 156, 422, 526, 583, 829, 1607, 1645
HspAI GCGC 1 cut(s) 602
Ksp22I TGATCA 1 cut(s) 1221
KspI CCGCGG 1 cut(s) 348
Kzo9I GATC 4 cut(s) 214, 865, 1221, 1290
LguI GCTCTTC 2 cut(s) 186, 321
LmnI GCTCC 2 cut(s) 1489, 1705
Lsp1109I GCAGC 8 cut(s) 7, 364, 683, 925, 940, 1342, 1538, 1550
LweI GCATC 5 cut(s) 432, 746, 1030, 1368, 1525
MabI ACCWGGT 1 cut(s) 810
MaeI CTAG 6 cut(s) 200, 392, 743, 942, 960, 1287
MaeII ACGT 1 cut(s) 1566
MaeIII GTNAC 3 cut(s) 137, 589, 836
MalI GATC 4 cut(s) 216, 867, 1223, 1292
MboI GATC 4 cut(s) 214, 865, 1221, 1290
MboII GAAGA 7 cut(s) 173, 308, 347, 451, 1247, 1361, 1677
MfeI CAATTG 3 cut(s) 557, 662, 1483
MflI RGATCY 1 cut(s) 1290
MhlI GDGCHC 3 cut(s) 181, 410, 494
MlsI TGGCCA 1 cut(s) 1563
MluNI TGGCCA 1 cut(s) 1563
MlyI GAGTC 4 cut(s) 347, 575, 722, 1691
MmeI TCCRAC 1 cut(s) 1662
Mox20I TGGCCA 1 cut(s) 1563
MroXI GAANNNNTTC 2 cut(s) 633, 1400
MscI TGGCCA 1 cut(s) 1563
MslI CAYNNNNRTG 1 cut(s) 1026
Msp20I TGGCCA 1 cut(s) 1563
MspA1I CMGCKG 4 cut(s) 347, 1333, 1516, 1541
MspI CCGG 1 cut(s) 1428
MspR9I CCNGG 4 cut(s) 225, 812, 1429, 1587
MunI CAATTG 3 cut(s) 557, 662, 1483
Mva1269I GAATGC 2 cut(s) 898, 1109
MvaI CCWGG 3 cut(s) 225, 812, 1587
MvnI CGCG 2 cut(s) 347, 1572
MwoI GCNNNNNNNGC 4 cut(s) 42, 830, 1535, 1569
NciI CCSGG 1 cut(s) 1429
NcoI CCATGG 1 cut(s) 825
NdeI CATATG 1 cut(s) 1345
NdeII GATC 4 cut(s) 214, 865, 1221, 1290
NlaIII CATG 8 cut(s) 80, 156, 422, 526, 583, 829, 1607, 1645
NlaIV GGNNCC 1 cut(s) 1292
NspI RCATGY 1 cut(s) 156
PaeR7I CTCGAG 1 cut(s) 1613
PciSI GCTCTTC 2 cut(s) 186, 321
PctI GAATGC 2 cut(s) 898, 1109
PdmI GAANNNNTTC 2 cut(s) 633, 1400
PfeI GAWTC 2 cut(s) 629, 981
PkrI GCNGC 8 cut(s) 22, 379, 673, 940, 955, 1332, 1528, 1540
PleI GAGTC 4 cut(s) 347, 574, 722, 1690
PpsI GAGTC 4 cut(s) 347, 574, 722, 1690
Psp124BI GAGCTC 1 cut(s) 181
Psp6I CCWGG 3 cut(s) 223, 810, 1585
PspEI GGTNACC 1 cut(s) 589
PspFI CCCAGC 1 cut(s) 759
PspGI CCWGG 3 cut(s) 223, 810, 1585
PspN4I GGNNCC 1 cut(s) 1292
PspPI GGNCC 3 cut(s) 193, 227, 721
PspXI VCTCGAGB 1 cut(s) 1613
PsuI RGATCY 1 cut(s) 1290
PvuII CAGCTG 3 cut(s) 1333, 1516, 1541
RsaI GTAC 2 cut(s) 1278, 1545
RsaNI GTAC 2 cut(s) 1277, 1544
RseI CAYNNNNRTG 1 cut(s) 1026
SacI GAGCTC 1 cut(s) 181
SacII CCGCGG 1 cut(s) 348
SapI GCTCTTC 2 cut(s) 186, 321
SatI GCNGC 8 cut(s) 21, 378, 672, 939, 954, 1331, 1527, 1539
Sau3AI GATC 4 cut(s) 214, 865, 1221, 1290
Sau96I GGNCC 3 cut(s) 193, 227, 721
SchI GAGTC 4 cut(s) 347, 575, 722, 1691
ScrFI CCNGG 4 cut(s) 225, 812, 1429, 1587
SduI GDGCHC 3 cut(s) 181, 410, 494
SexAI ACCWGGT 1 cut(s) 810
SfaNI GCATC 5 cut(s) 432, 746, 1030, 1368, 1525
Sfr274I CTCGAG 1 cut(s) 1613
Sfr303I CCGCGG 1 cut(s) 348
SgrBI CCGCGG 1 cut(s) 348
SinI GGWCC 3 cut(s) 193, 227, 721
SlaI CTCGAG 1 cut(s) 1613
SmiMI CAYNNNNRTG 1 cut(s) 1026
SmlI CTYRAG 2 cut(s) 1097, 1613
SmoI CTYRAG 2 cut(s) 1097, 1613
SsiI CCGC 5 cut(s) 345, 347, 587, 616, 1572
SspI AATATT 1 cut(s) 475
SspMI CTAG 6 cut(s) 200, 392, 743, 942, 960, 1287
SstI GAGCTC 1 cut(s) 181
StyD4I CCNGG 4 cut(s) 223, 810, 1427, 1585
StyI CCWWGG 2 cut(s) 825, 1286
TaaI ACNGT 2 cut(s) 1010, 1281
TaiI ACGT 1 cut(s) 1569
TaqI TCGA 3 cut(s) 868, 1614, 1675
TatI WGTACW 2 cut(s) 1276, 1543
TfiI GAWTC 2 cut(s) 629, 981
TscAI CASTG 1 cut(s) 254
TseI GCWGC 8 cut(s) 20, 377, 671, 938, 953, 1330, 1526, 1538
TspDTI ATGAA 8 cut(s) 106, 682, 870, 1077, 1198, 1248, 1362, 1658
TspRI CASTG 1 cut(s) 254
VpaK11BI GGWCC 3 cut(s) 193, 227, 721
XapI RAATTY 1 cut(s) 533
XbaI TCTAGA 2 cut(s) 391, 959
XceI RCATGY 1 cut(s) 156
XhoI CTCGAG 1 cut(s) 1613
XmaJI CCTAGG 1 cut(s) 1286
XmnI GAANNNNTTC 2 cut(s) 633, 1400
XspI CTAG 6 cut(s) 200, 392, 743, 942, 960, 1287
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.