Rorug03G0015900

3'-5' exonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0003918
Physical Location & Seq
Reverse (-)
1274181 .. 1275152
972 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0015900.1

Sequence Viewer

Length: 972 bp
ATGGATATCCTGTTGCTTCTGTGTTTCTTGCTTGTTCTTGTACCAAATACCAAAGCAGATAATCATCATGTTCGTCAGCACTGCCCGAAATCAAGGTGTAGCCATGATGGTCCCCTGATCCAGTTTCCCTTCCGTCTAAAAGGCGATCCCCCCCACTGTGGAAATCCAGAGTTTGAGGTTTCTTGCAGCAGAAGCAACAACACCAATTTGACAATGATTCAGCTCTCCTCATCTTCAGGGCTGTTTCCCATCCAAAAAATCGATTACAAGAATAGATATTTTGATGTCTACGATGGTGATGGTTGCATGGCGAGCCGCCTCCTTAATCTTACAATTTTTACTTCATCCAGCTTGCTCTTTTATCCACTAGCTTATCACTGGAGCAGTGAGAACAATAACAGTTGCACTGAAAATTTCGGTCAACCTCCCTATCTGGATTCCCAGCTGTCTCCCTATTGCGCGCAATCTCCCTATTCAGATTCCGCCCAATCTCCCTATCCAGATTCCGCCCAATCTCCCTATCCAGATTCCACTCAGTATTTTCAAGAATTTGTGTTATTAAATTGTTCCACAACACAAAATGTTAGGGGATGGGGAAACAACTACATCCCAGTCAGATGTCTCAGTGTCCCAGGGCACCATCAAGTTCTTGCTGTTGAATCCTCAATTTCTATAGCTGACTTGCCGTTGCCGGTGTCAACTTGCACTACTTTGAAAAAAAAATTCTATCTTCCACTGAGAAGTGTAGATGACGATGGTAACGACTGTGCACCGGACAACGTTTTATCACTCGAGTGGAAACCATGGGTTTATTTCCCAGAATGTCATAACTGCAAAGGAGAGTGCTCTATGGATTGCAGGAGCAATCGAATGGAGTGCTATCCTCATCCGGCTGCATATCCCTATGATCAGTATGATCCCTATTATCAAGAACAAATGCCAAAGGGTATGCTGTCTAATCACATAGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_pol_A_exo1

Protein Analysis

323

Amino Acids

36.63

Weight (kDa)

5.48

Isoelectric Point (pI)

61.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 28 - 98 1e-15 Wall-associated receptor kinase galacturonan-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 636
AccI GTMKAC 1 cut(s) 288
AccII CGCG 1 cut(s) 461
AciI CCGC 3 cut(s) 316, 483, 507
AclI AACGTT 1 cut(s) 780
AclWI GGATC 3 cut(s) 112, 140, 911
AcsI RAATTY 3 cut(s) 412, 548, 722
AcuI CTGAAG 1 cut(s) 219
AfaI GTAC 1 cut(s) 42
AfiI CCNNNNNNNGG 1 cut(s) 158
AgsI TTSAA 3 cut(s) 545, 659, 715
AjnI CCWGG 1 cut(s) 631
AleI CACNNNNGTG 1 cut(s) 793
AluBI AGCT 6 cut(s) 223, 351, 371, 445, 677, 968
AluI AGCT 6 cut(s) 223, 351, 371, 445, 677, 968
Alw21I GWGCWC 2 cut(s) 772, 848
Alw26I GTCTC 2 cut(s) 453, 626
Alw44I GTGCAC 1 cut(s) 768
AlwI GGATC 3 cut(s) 112, 140, 911
Ama87I CYCGRG 1 cut(s) 791
ApaLI GTGCAC 1 cut(s) 768
ApeKI GCWGC 2 cut(s) 186, 893
ApoI RAATTY 3 cut(s) 412, 548, 722
AspLEI GCGC 2 cut(s) 461, 463
AspS9I GGNCC 1 cut(s) 110
AsuHPI GGTGA 1 cut(s) 308
AvaI CYCGRG 1 cut(s) 791
AvaII GGWCC 1 cut(s) 110
BaeGI GKGCMC 2 cut(s) 639, 772
BanI GGYRCC 1 cut(s) 636
Bbv12I GWGCWC 2 cut(s) 772, 848
BbvI GCAGC 2 cut(s) 198, 880
BccI CCATC 7 cut(s) 101, 257, 287, 293, 585, 648, 749
BceAI ACGGC 1 cut(s) 670
BcgI CGANNNNNNTGC 2 cut(s) 858, 892
BciT130I CCWGG 1 cut(s) 633
BclI TGATCA 1 cut(s) 907
BcoDI GTCTC 2 cut(s) 453, 626
BfaI CTAG 1 cut(s) 368
BfmI CTRYAG 1 cut(s) 672
BisI GCNGC 3 cut(s) 187, 316, 894
BlsI GCNGC 3 cut(s) 188, 317, 895
Bme1390I CCNGG 1 cut(s) 633
Bme18I GGWCC 1 cut(s) 110
BmeT110I CYCGRG 1 cut(s) 791
BmgT120I GGNCC 1 cut(s) 110
BmiI GGNNCC 2 cut(s) 112, 638
BmrFI CCNGG 1 cut(s) 633
BmrI ACTGGG 1 cut(s) 605
BmuI ACTGGG 1 cut(s) 605
BpmI CTGGAG 1 cut(s) 400
Bsa29I ATCGAT 1 cut(s) 261
BsaBI GATNNNNATC 1 cut(s) 63
BsaJI CCNNGG 3 cut(s) 631, 632, 803
BsaWI WCCGGW 1 cut(s) 772
Bsc4I CCNNNNNNNGG 1 cut(s) 158
Bse118I RCCGGY 1 cut(s) 691
Bse1I ACTGG 3 cut(s) 121, 383, 611
Bse8I GATNNNNATC 1 cut(s) 63
BseBI CCWGG 1 cut(s) 633
BseCI ATCGAT 1 cut(s) 261
BseDI CCNNGG 3 cut(s) 631, 632, 803
BseGI GGATG 5 cut(s) 249, 344, 596, 606, 886
BseJI GATNNNNATC 1 cut(s) 63
BseLI CCNNNNNNNGG 1 cut(s) 158
BseMII CTCAG 3 cut(s) 548, 637, 728
BseNI ACTGG 3 cut(s) 121, 383, 611
BsePI GCGCGC 1 cut(s) 459
BseRI GAGGAG 1 cut(s) 217
BseSI GKGCMC 2 cut(s) 639, 772
BseXI GCAGC 2 cut(s) 198, 880
BseYI CCCAGC 1 cut(s) 441
Bsh1236I CGCG 1 cut(s) 461
BshNI GGYRCC 1 cut(s) 636
BshVI ATCGAT 1 cut(s) 261
BsiHKAI GWGCWC 2 cut(s) 772, 848
BsiHKCI CYCGRG 1 cut(s) 791
BsiSI CCGG 3 cut(s) 692, 773, 890
BslFI GGGAC 2 cut(s) 96, 614
BslI CCNNNNNNNGG 1 cut(s) 158
BsmAI GTCTC 2 cut(s) 453, 626
BsmFI GGGAC 2 cut(s) 96, 614
BsoBI CYCGRG 1 cut(s) 791
Bsp1286I GDGCHC 3 cut(s) 639, 772, 848
Bsp143I GATC 4 cut(s) 117, 145, 907, 916
Bsp19I CCATGG 1 cut(s) 803
BspACI CCGC 3 cut(s) 316, 483, 507
BspCNI CTCAG 3 cut(s) 547, 636, 729
BspDI ATCGAT 1 cut(s) 261
BspFNI CGCG 1 cut(s) 461
BspLI GGNNCC 2 cut(s) 112, 638
BspPI GGATC 3 cut(s) 112, 140, 911
BspT107I GGYRCC 1 cut(s) 636
BsrFI RCCGGY 1 cut(s) 691
BsrI ACTGG 3 cut(s) 121, 383, 611
BssAI RCCGGY 1 cut(s) 691
BssECI CCNNGG 3 cut(s) 631, 632, 803
BssHII GCGCGC 1 cut(s) 459
BssMI GATC 4 cut(s) 117, 145, 907, 916
BssT1I CCWWGG 1 cut(s) 803
Bst2UI CCWGG 1 cut(s) 633
Bst4CI ACNGT 3 cut(s) 158, 401, 767
BstC8I GCNNGC 3 cut(s) 313, 353, 461
BstDEI CTNAG 4 cut(s) 534, 623, 737, 969
BstDSI CCRYGG 1 cut(s) 803
BstF5I GGATG 5 cut(s) 249, 344, 596, 606, 886
BstFNI CGCG 1 cut(s) 461
BstHHI GCGC 2 cut(s) 461, 463
BstKTI GATC 4 cut(s) 120, 148, 910, 919
BstMAI GTCTC 2 cut(s) 453, 626
BstMBI GATC 4 cut(s) 117, 145, 907, 916
BstMWI GCNNNNNNNGC 2 cut(s) 192, 312
BstNI CCWGG 1 cut(s) 633
BstSCI CCNGG 1 cut(s) 631
BstSFI CTRYAG 1 cut(s) 672
BstSLI GKGCMC 2 cut(s) 639, 772
BstUI CGCG 1 cut(s) 461
BstV1I GCAGC 2 cut(s) 198, 880
Bsu15I ATCGAT 1 cut(s) 261
BsuTUI ATCGAT 1 cut(s) 261
BtgI CCRYGG 1 cut(s) 803
BtsCI GGATG 5 cut(s) 249, 344, 596, 606, 886
BtsI GCAGTG 2 cut(s) 79, 391
BtsIMutI CAGTG 7 cut(s) 79, 154, 376, 391, 405, 631, 734
Cac8I GCNNGC 3 cut(s) 313, 353, 461
CfoI GCGC 2 cut(s) 461, 463
Cfr10I RCCGGY 1 cut(s) 691
Cfr13I GGNCC 1 cut(s) 110
ClaI ATCGAT 1 cut(s) 261
Csp6I GTAC 1 cut(s) 41
CviAII CATG 4 cut(s) 68, 104, 307, 804
CviQI GTAC 1 cut(s) 41
DdeI CTNAG 4 cut(s) 534, 623, 737, 969
DpnI GATC 4 cut(s) 119, 147, 909, 918
DpnII GATC 4 cut(s) 117, 145, 907, 916
EciI GGCGGA 2 cut(s) 472, 496
Eco130I CCWWGG 1 cut(s) 803
Eco32I GATATC 1 cut(s) 7
Eco47I GGWCC 1 cut(s) 110
Eco57I CTGAAG 1 cut(s) 219
Eco88I CYCGRG 1 cut(s) 791
EcoRII CCWGG 1 cut(s) 631
EcoRV GATATC 1 cut(s) 7
EcoT14I CCWWGG 1 cut(s) 803
ErhI CCWWGG 1 cut(s) 803
FaeI CATG 4 cut(s) 71, 107, 310, 807
FaqI GGGAC 2 cut(s) 96, 614
FatI CATG 4 cut(s) 67, 103, 306, 803
FbaI TGATCA 1 cut(s) 907
FblI GTMKAC 1 cut(s) 288
Fnu4HI GCNGC 3 cut(s) 187, 316, 894
FokI GGATG 5 cut(s) 236, 331, 593, 603, 873
Fsp4HI GCNGC 3 cut(s) 187, 316, 894
FspBI CTAG 1 cut(s) 368
GlaI GCGC 2 cut(s) 460, 462
GluI GCNGC 3 cut(s) 187, 316, 894
GsaI CCCAGC 1 cut(s) 445
GsuI CTGGAG 1 cut(s) 400
HapII CCGG 3 cut(s) 692, 773, 890
HhaI GCGC 2 cut(s) 461, 463
Hin1II CATG 4 cut(s) 71, 107, 310, 807
Hin6I GCGC 2 cut(s) 459, 461
HinP1I GCGC 2 cut(s) 459, 461
HincII GTYRAC 2 cut(s) 422, 699
HindII GTYRAC 2 cut(s) 422, 699
HinfI GANTC 6 cut(s) 217, 437, 479, 503, 527, 659
HpaII CCGG 3 cut(s) 692, 773, 890
HphI GGTGA 1 cut(s) 308
Hpy166II GTNNAC 4 cut(s) 289, 422, 699, 770
Hpy188I TCNGA 2 cut(s) 478, 617
Hpy188III TCNNGA 6 cut(s) 167, 434, 500, 524, 545, 929
Hpy8I GTNNAC 4 cut(s) 289, 422, 699, 770
HpyAV CCTTC 1 cut(s) 139
HpyCH4III ACNGT 3 cut(s) 158, 401, 767
HpyCH4IV ACGT 1 cut(s) 780
HpyCH4V TGCA 8 cut(s) 186, 306, 405, 705, 770, 834, 858, 896
HpyF10VI GCNNNNNNNGC 2 cut(s) 192, 312
HpyF3I CTNAG 4 cut(s) 534, 623, 737, 969
HpySE526I ACGT 1 cut(s) 780
Hsp92II CATG 4 cut(s) 71, 107, 310, 807
HspAI GCGC 2 cut(s) 459, 461
Ksp22I TGATCA 1 cut(s) 907
Kzo9I GATC 4 cut(s) 117, 145, 907, 916
LmnI GCTCC 2 cut(s) 381, 861
Lsp1109I GCAGC 2 cut(s) 198, 880
MaeI CTAG 1 cut(s) 368
MaeII ACGT 1 cut(s) 780
MaeIII GTNAC 1 cut(s) 758
MalI GATC 4 cut(s) 119, 147, 909, 918
MboI GATC 4 cut(s) 117, 145, 907, 916
MboII GAAGA 2 cut(s) 225, 722
MhlI GDGCHC 3 cut(s) 639, 772, 848
MluCI AATT 7 cut(s) 205, 333, 412, 548, 562, 666, 722
MnlI CCTC 6 cut(s) 169, 238, 329, 435, 673, 894
MseI TTAA 2 cut(s) 324, 560
MslI CAYNNNNRTG 1 cut(s) 793
MspA1I CMGCKG 1 cut(s) 445
MspI CCGG 3 cut(s) 692, 773, 890
MspR9I CCNGG 1 cut(s) 633
MvaI CCWGG 1 cut(s) 633
MvnI CGCG 1 cut(s) 461
MwoI GCNNNNNNNGC 2 cut(s) 192, 312
NcoI CCATGG 1 cut(s) 803
NdeII GATC 4 cut(s) 117, 145, 907, 916
NlaIII CATG 4 cut(s) 71, 107, 310, 807
NlaIV GGNNCC 2 cut(s) 112, 638
OliI CACNNNNGTG 1 cut(s) 793
PaeR7I CTCGAG 1 cut(s) 791
PasI CCCWGGG 1 cut(s) 632
PauI GCGCGC 1 cut(s) 459
PcsI WCGNNNNNNNCGW 1 cut(s) 759
PfeI GAWTC 6 cut(s) 217, 437, 479, 503, 527, 659
PkrI GCNGC 3 cut(s) 188, 317, 895
Psp1406I AACGTT 1 cut(s) 780
Psp6I CCWGG 1 cut(s) 631
PspFI CCCAGC 1 cut(s) 441
PspGI CCWGG 1 cut(s) 631
PspN4I GGNNCC 2 cut(s) 112, 638
PspPI GGNCC 1 cut(s) 110
PspXI VCTCGAGB 1 cut(s) 791
PteI GCGCGC 1 cut(s) 459
PvuII CAGCTG 1 cut(s) 445
RsaI GTAC 1 cut(s) 42
RsaNI GTAC 1 cut(s) 41
RseI CAYNNNNRTG 1 cut(s) 793
SaqAI TTAA 2 cut(s) 324, 560
SatI GCNGC 3 cut(s) 187, 316, 894
Sau3AI GATC 4 cut(s) 117, 145, 907, 916
Sau96I GGNCC 1 cut(s) 110
ScrFI CCNGG 1 cut(s) 633
SduI GDGCHC 3 cut(s) 639, 772, 848
SfcI CTRYAG 1 cut(s) 672
Sfr274I CTCGAG 1 cut(s) 791
SinI GGWCC 1 cut(s) 110
SlaI CTCGAG 1 cut(s) 791
SmiMI CAYNNNNRTG 1 cut(s) 793
SmlI CTYRAG 1 cut(s) 791
SmoI CTYRAG 1 cut(s) 791
Sse9I AATT 7 cut(s) 205, 333, 412, 548, 562, 666, 722
SsiI CCGC 3 cut(s) 316, 483, 507
SspMI CTAG 1 cut(s) 368
StyD4I CCNGG 1 cut(s) 631
StyI CCWWGG 1 cut(s) 803
TaaI ACNGT 3 cut(s) 158, 401, 767
TaiI ACGT 1 cut(s) 783
TaqI TCGA 3 cut(s) 261, 792, 868
TaqII GACCGA 1 cut(s) 407
TasI AATT 7 cut(s) 205, 333, 412, 548, 562, 666, 722
TauI GCSGC 1 cut(s) 318
TfiI GAWTC 6 cut(s) 217, 437, 479, 503, 527, 659
Tru1I TTAA 2 cut(s) 324, 560
Tru9I TTAA 2 cut(s) 324, 560
TscAI CASTG 7 cut(s) 86, 161, 383, 391, 412, 631, 741
TseI GCWGC 2 cut(s) 186, 893
TspDTI ATGAA 1 cut(s) 333
TspGWI ACGGA 1 cut(s) 122
TspRI CASTG 7 cut(s) 86, 161, 383, 391, 412, 631, 741
VneI GTGCAC 1 cut(s) 768
VpaK11BI GGWCC 1 cut(s) 110
XapI RAATTY 3 cut(s) 412, 548, 722
XhoI CTCGAG 1 cut(s) 791
XmiI GTMKAC 1 cut(s) 288
XspI CTAG 1 cut(s) 368
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.