RLG00000001137

3'-5' exonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0003918
Physical Location & Seq
Reverse (-)
10705280 .. 10706631
1352 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000001137

Sequence Viewer

Length: 1263 bp
ATGCACCAGAGTGAAAAGAATGATTGCAGTTTTGACATGGCACAAAAGTTTGTTGAGCAATATATTTATAAGTTGATAGAGTCTGAATCATATATGACTGCAGTTACTCTATTAGAGCACTTCTCTATCCGCCAGTTTGGCAAATCTTTTCTCCTCAGTATGATAGAAAGCAAGCAATTCAAAGCAGCGGACAAATGGGCAACGTTCATCGGAAATCCCATGTTATATGTTCTTGTCCATGAGTACATCGACAGGAATATGCTAAAGAATGCTAAGGAGGTTATGCTGAAAAACAATTTCCAACAGGAATTCCCTGATGTTTGTCACAAGTACAAAGAAAGATCACTAAAGAAACTAGCAATAAAAGGATGCTGGGACGTTGCAGAATCAAGGACAAAAGGTAATAGGCAACTTCTTGAATATCTGGTTTATTTAGCAATGGAGGCTGGTTACTCAGAGAAGGTTCATGAAATATGTGATCGATACTCACTGGAAGGTCTCTTCAATGTCAAAGTGCCTGAGCCAAATCTTTGCTATTTGCGTCTCGATGAGCTGGCTGTAGAAGACATCATTTGGGTAGATCAAGTTGATGCTTTACATAACGCAGCCTGTCATATTGAAGAAGCCGAAGTCATAGGTCTTGATTGTGAATGGAGACCAAATTATGAAAAGGGAGGAGAAGAAAATCAAGTTTCTATCATGCAACTTGCTTCTGGGAAGGTGGTTTTCATCCTTGACTTGATCAAGTTATTTGAAGATGTGCCTGATGTCTTGGATGACTGCTTAACTCGCATTTTTCAGTCTCCTAAGATACTAAAACTTGGTTATGCTTTCGTCTATGATGCAAGGCATCTAGCTGATTCATATGGAGGGTTAAACTGTTTCAATCACTATCGGATGCTTCTGGACATCCAGCATGTATTTAAAGAACAAGGGGGTGGTCTATCGGGGCTTGTGCAGAAAATACTGGGAGTTGGTTTGAACAAGACAAGAAGGAACAGCAATTGGGAGCAACGGCCTTTGACGCCTAATCAGCTGGAATATGCAGCCATCGATGCAGCTGTACTGCTTCACATATTTGACCGTGTTTGCAGTCATTCTCAAACTGCTGATGGAGAAAAACTTGGAAAACTCGACTGGAAATCCTGCATCTGTCCCCGCATGGATGACATCAAGAAGTCCAAAAAGGGACATCATCTCCTCATGCCGCCAGTGAAATCACCGTGGACCGCCTTGTGGACTTTAGAGATGATTAGACTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_pol_A_exo1

Protein Analysis

421

Amino Acids

48.5

Weight (kDa)

6.08

Isoelectric Point (pI)

40.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_pol_A_exo1 PF01612 191 - 362 1e-20 3'-5' exonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 69
AciI CCGC 5 cut(s) 130, 188, 1159, 1208, 1230
AclI AACGTT 1 cut(s) 203
AcsI RAATTY 1 cut(s) 308
AcyI GRCGYC 1 cut(s) 1025
AfaI GTAC 3 cut(s) 245, 332, 1065
AfiI CCNNNNNNNGG 1 cut(s) 1236
AgsI TTSAA 7 cut(s) 181, 419, 505, 620, 755, 886, 982
AleI CACNNNNGTG 1 cut(s) 9
AluBI AGCT 4 cut(s) 553, 857, 1036, 1061
AluI AGCT 4 cut(s) 553, 857, 1036, 1061
Alw21I GWGCWC 1 cut(s) 120
Alw26I GTCTC 4 cut(s) 503, 548, 649, 807
AoxI GGCC 1 cut(s) 1016
ApeKI GCWGC 4 cut(s) 185, 605, 1046, 1058
ApoI RAATTY 1 cut(s) 308
AspS9I GGNCC 1 cut(s) 1227
AsuHPI GGTGA 1 cut(s) 1212
AvaII GGWCC 1 cut(s) 1227
BbsI GAAGAC 1 cut(s) 570
Bbv12I GWGCWC 1 cut(s) 120
BbvI GCAGC 4 cut(s) 197, 617, 1058, 1070
BccI CCATC 2 cut(s) 1058, 1106
BceAI ACGGC 1 cut(s) 1031
BclI TGATCA 1 cut(s) 741
BcoDI GTCTC 4 cut(s) 503, 548, 649, 807
BfaI CTAG 2 cut(s) 356, 854
BfmI CTRYAG 2 cut(s) 99, 558
BglI GCCNNNNNGGC 1 cut(s) 138
BisI GCNGC 5 cut(s) 186, 606, 1047, 1059, 1208
BlsI GCNGC 5 cut(s) 187, 607, 1048, 1060, 1209
Bme18I GGWCC 1 cut(s) 1227
BmgT120I GGNCC 1 cut(s) 1227
BmrI ACTGGG 1 cut(s) 977
BmsI GCATC 7 cut(s) 359, 580, 832, 859, 888, 1045, 1158
BmuI ACTGGG 1 cut(s) 977
BpiI GAAGAC 1 cut(s) 570
BplI GAGNNNNNCTC 2 cut(s) 107, 139
Bpu10I CCTNAGC 2 cut(s) 273, 519
Bsa29I ATCGAT 2 cut(s) 481, 1053
BsaHI GRCGYC 1 cut(s) 1025
BsaI GGTCTC 2 cut(s) 503, 649
BsaJI CCNNGG 1 cut(s) 1223
BsaXI ACNNNNNCTCC 2 cut(s) 1182, 1212
Bsc4I CCNNNNNNNGG 1 cut(s) 1236
Bse1I ACTGG 5 cut(s) 133, 495, 972, 1142, 1211
Bse3DI GCAATG 1 cut(s) 444
BseCI ATCGAT 2 cut(s) 481, 1053
BseDI CCNNGG 1 cut(s) 1223
BseGI GGATG 6 cut(s) 374, 729, 781, 903, 909, 1171
BseLI CCNNNNNNNGG 1 cut(s) 1236
BseMI GCAATG 1 cut(s) 444
BseMII CTCAG 3 cut(s) 169, 468, 510
BseNI ACTGG 5 cut(s) 133, 495, 972, 1142, 1211
BseRI GAGGAG 3 cut(s) 143, 690, 1190
BseXI GCAGC 4 cut(s) 197, 617, 1058, 1070
BseYI CCCAGC 1 cut(s) 372
BsgI GTGCAG 1 cut(s) 977
BshFI GGCC 1 cut(s) 1018
BshVI ATCGAT 2 cut(s) 481, 1053
BsiHKAI GWGCWC 1 cut(s) 120
BslFI GGGAC 3 cut(s) 389, 1140, 1203
BslI CCNNNNNNNGG 1 cut(s) 1236
BsmAI GTCTC 4 cut(s) 503, 548, 649, 807
BsmBI CGTCTC 1 cut(s) 548
BsmFI GGGAC 3 cut(s) 389, 1140, 1203
BsmI GAATGC 1 cut(s) 274
BsnI GGCC 1 cut(s) 1018
Bso31I GGTCTC 2 cut(s) 503, 649
Bsp1286I GDGCHC 1 cut(s) 120
Bsp143I GATC 4 cut(s) 341, 478, 580, 741
BspACI CCGC 5 cut(s) 130, 188, 1159, 1208, 1230
BspANI GGCC 1 cut(s) 1018
BspCNI CTCAG 3 cut(s) 168, 467, 511
BspDI ATCGAT 2 cut(s) 481, 1053
BspHI TCATGA 1 cut(s) 466
BspMAI CTGCAG 1 cut(s) 103
BspTNI GGTCTC 2 cut(s) 503, 649
BsrDI GCAATG 1 cut(s) 444
BsrI ACTGG 5 cut(s) 133, 495, 972, 1142, 1211
BssECI CCNNGG 1 cut(s) 1223
BssMI GATC 4 cut(s) 341, 478, 580, 741
BssNI GRCGYC 1 cut(s) 1025
Bst4CI ACNGT 3 cut(s) 881, 1085, 1224
Bst6I CTCTTC 1 cut(s) 506
BstACI GRCGYC 1 cut(s) 1025
BstC8I GCNNGC 2 cut(s) 173, 555
BstDEI CTNAG 5 cut(s) 155, 273, 454, 519, 807
BstDSI CCRYGG 1 cut(s) 1223
BstF5I GGATG 6 cut(s) 374, 729, 781, 903, 909, 1171
BstKTI GATC 4 cut(s) 344, 481, 583, 744
BstMAI GTCTC 4 cut(s) 503, 548, 649, 807
BstMBI GATC 4 cut(s) 341, 478, 580, 741
BstMWI GCNNNNNNNGC 6 cut(s) 138, 443, 789, 1024, 1033, 1055
BstNSI RCATGY 1 cut(s) 920
BstSFI CTRYAG 2 cut(s) 99, 558
BstV1I GCAGC 4 cut(s) 197, 617, 1058, 1070
BstV2I GAAGAC 1 cut(s) 570
Bsu15I ATCGAT 2 cut(s) 481, 1053
BsuRI GGCC 1 cut(s) 1018
BsuTUI ATCGAT 2 cut(s) 481, 1053
BtgI CCRYGG 1 cut(s) 1223
BtsCI GGATG 6 cut(s) 374, 729, 781, 903, 909, 1171
BtsIMutI CAGTG 2 cut(s) 488, 1218
Cac8I GCNNGC 2 cut(s) 173, 555
CciI TCATGA 1 cut(s) 466
Cfr13I GGNCC 1 cut(s) 1227
ClaI ATCGAT 2 cut(s) 481, 1053
CseI GACGC 2 cut(s) 530, 1033
Csp6I GTAC 3 cut(s) 244, 331, 1064
CviAII CATG 8 cut(s) 37, 220, 239, 467, 700, 917, 1162, 1204
CviQI GTAC 3 cut(s) 244, 331, 1064
DdeI CTNAG 5 cut(s) 155, 273, 454, 519, 807
DpnI GATC 4 cut(s) 343, 480, 582, 743
DpnII GATC 4 cut(s) 341, 478, 580, 741
DraI TTTAAA 1 cut(s) 925
Eam1104I CTCTTC 1 cut(s) 506
EarI CTCTTC 1 cut(s) 506
EciI GGCGGA 1 cut(s) 119
Eco31I GGTCTC 2 cut(s) 503, 649
Eco47I GGWCC 1 cut(s) 1227
EcoRI GAATTC 1 cut(s) 308
Esp3I CGTCTC 1 cut(s) 548
FaeI CATG 8 cut(s) 40, 223, 242, 470, 703, 920, 1165, 1207
FaqI GGGAC 3 cut(s) 389, 1140, 1203
FatI CATG 8 cut(s) 36, 219, 238, 466, 699, 916, 1161, 1203
FauI CCCGC 1 cut(s) 1166
FauNDI CATATG 1 cut(s) 865
FbaI TGATCA 1 cut(s) 741
Fnu4HI GCNGC 5 cut(s) 186, 606, 1047, 1059, 1208
FokI GGATG 6 cut(s) 381, 716, 788, 896, 910, 1178
Fsp4HI GCNGC 5 cut(s) 186, 606, 1047, 1059, 1208
FspBI CTAG 2 cut(s) 356, 854
GluI GCNGC 5 cut(s) 186, 606, 1047, 1059, 1208
GsaI CCCAGC 1 cut(s) 376
HaeIII GGCC 1 cut(s) 1018
HgaI GACGC 2 cut(s) 530, 1033
Hin1I GRCGYC 1 cut(s) 1025
Hin1II CATG 8 cut(s) 40, 223, 242, 470, 703, 920, 1165, 1207
HinfI GANTC 5 cut(s) 80, 86, 386, 860, 1257
HphI GGTGA 1 cut(s) 1212
Hpy166II GTNNAC 2 cut(s) 1227, 1239
Hpy188I TCNGA 4 cut(s) 85, 212, 457, 897
Hpy188III TCNNGA 6 cut(s) 416, 467, 545, 641, 905, 1174
Hpy8I GTNNAC 2 cut(s) 1227, 1239
HpyAV CCTTC 4 cut(s) 454, 488, 712, 987
HpyCH4III ACNGT 3 cut(s) 881, 1085, 1224
HpyCH4IV ACGT 2 cut(s) 203, 378
HpyF10VI GCNNNNNNNGC 6 cut(s) 138, 443, 789, 1024, 1033, 1055
HpyF3I CTNAG 5 cut(s) 155, 273, 454, 519, 807
HpySE526I ACGT 2 cut(s) 203, 378
Hsp92I GRCGYC 1 cut(s) 1025
Hsp92II CATG 8 cut(s) 40, 223, 242, 470, 703, 920, 1165, 1207
Ksp22I TGATCA 1 cut(s) 741
Kzo9I GATC 4 cut(s) 341, 478, 580, 741
LmnI GCTCC 1 cut(s) 1009
Lsp1109I GCAGC 4 cut(s) 197, 617, 1058, 1070
LweI GCATC 7 cut(s) 359, 580, 832, 859, 888, 1045, 1158
MaeI CTAG 2 cut(s) 356, 854
MaeII ACGT 2 cut(s) 203, 378
MaeIII GTNAC 3 cut(s) 103, 323, 449
MalI GATC 4 cut(s) 343, 480, 582, 743
MboI GATC 4 cut(s) 341, 478, 580, 741
MboII GAAGA 5 cut(s) 493, 575, 632, 692, 767
MfeI CAATTG 1 cut(s) 1003
MhlI GDGCHC 1 cut(s) 120
MluCI AATT 5 cut(s) 176, 295, 308, 661, 1003
MlyI GAGTC 2 cut(s) 89, 1251
MmeI TCCRAC 1 cut(s) 325
MnlI CCTC 6 cut(s) 164, 271, 436, 668, 863, 1211
MseI TTAA 3 cut(s) 785, 875, 924
MslI CAYNNNNRTG 1 cut(s) 9
MspA1I CMGCKG 3 cut(s) 188, 1036, 1061
MunI CAATTG 1 cut(s) 1003
Mva1269I GAATGC 1 cut(s) 274
MwoI GCNNNNNNNGC 6 cut(s) 138, 443, 789, 1024, 1033, 1055
NdeI CATATG 1 cut(s) 865
NdeII GATC 4 cut(s) 341, 478, 580, 741
NlaIII CATG 8 cut(s) 40, 223, 242, 470, 703, 920, 1165, 1207
NmuCI GTSAC 1 cut(s) 323
NspI RCATGY 1 cut(s) 920
OliI CACNNNNGTG 1 cut(s) 9
PagI TCATGA 1 cut(s) 466
PctI GAATGC 1 cut(s) 274
PfeI GAWTC 3 cut(s) 86, 386, 860
PkrI GCNGC 5 cut(s) 187, 607, 1048, 1060, 1209
PleI GAGTC 2 cut(s) 88, 1251
PpsI GAGTC 2 cut(s) 88, 1251
PsiI TTATAA 1 cut(s) 69
Psp1406I AACGTT 1 cut(s) 203
PspFI CCCAGC 1 cut(s) 372
PspPI GGNCC 1 cut(s) 1227
PstI CTGCAG 1 cut(s) 103
PvuII CAGCTG 2 cut(s) 1036, 1061
RsaI GTAC 3 cut(s) 245, 332, 1065
RsaNI GTAC 3 cut(s) 244, 331, 1064
RseI CAYNNNNRTG 1 cut(s) 9
SaqAI TTAA 3 cut(s) 785, 875, 924
SatI GCNGC 5 cut(s) 186, 606, 1047, 1059, 1208
Sau3AI GATC 4 cut(s) 341, 478, 580, 741
Sau96I GGNCC 1 cut(s) 1227
SchI GAGTC 2 cut(s) 89, 1251
SduI GDGCHC 1 cut(s) 120
SfaNI GCATC 7 cut(s) 359, 580, 832, 859, 888, 1045, 1158
SfcI CTRYAG 2 cut(s) 99, 558
SinI GGWCC 1 cut(s) 1227
SmiMI CAYNNNNRTG 1 cut(s) 9
Sse9I AATT 5 cut(s) 176, 295, 308, 661, 1003
SsiI CCGC 5 cut(s) 130, 188, 1159, 1208, 1230
SspMI CTAG 2 cut(s) 356, 854
TaaI ACNGT 3 cut(s) 881, 1085, 1224
TaiI ACGT 2 cut(s) 206, 381
TaqI TCGA 5 cut(s) 249, 481, 546, 1053, 1134
TasI AATT 5 cut(s) 176, 295, 308, 661, 1003
TatI WGTACW 3 cut(s) 243, 330, 1063
TauI GCSGC 1 cut(s) 1210
TfiI GAWTC 3 cut(s) 86, 386, 860
Tru1I TTAA 3 cut(s) 785, 875, 924
Tru9I TTAA 3 cut(s) 785, 875, 924
TscAI CASTG 2 cut(s) 495, 1218
TseFI GTSAC 1 cut(s) 323
TseI GCWGC 4 cut(s) 185, 605, 1046, 1058
Tsp45I GTSAC 1 cut(s) 323
TspDTI ATGAA 6 cut(s) 196, 455, 483, 681, 718, 852
TspRI CASTG 2 cut(s) 495, 1218
VpaK11BI GGWCC 1 cut(s) 1227
XapI RAATTY 1 cut(s) 308
XceI RCATGY 1 cut(s) 920
XspI CTAG 2 cut(s) 356, 854
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.