pycom04g17290

3'-5' exonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0003918
Physical Location & Seq
Reverse (-)
19727774 .. 19730334
2561 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g17290.2

Sequence Viewer

Length: 1374 bp
ATGGAAGCAAAGGACCTCGCTGCTCAGTTGTTTCTCGATATTGTTGGGGGCTCTGTCAACCATGATGAGAGGATAGTTGAGAAGATAGTTGAAGTTTATGATGTTCAGTTGGCAAACATTGATAAAGTAATGTGCCTAAATGCAAAGAATAATTGCAGATTTGACAATGCAAAAGAGTTTGTTGAGCAATATATATTTGGACTGATAGAGTCTCAATCATATATGGCTGCGGTTACTTTATTAGAGCACTTCTCTATCCACCAGACTGGCCAATCGTTTCTCCATCGTATGATAGAAAGCAGACAGTTGAAAGCAGCAGATAAATGGGCAACATTCATGGGAAAGCCAATGTTATGTATTCTTGTCCAGGAGTACTTTGGCAGGAACATGCTAAAAAATGCTTATGACATTATACAAAAAAACAATCTCCGACATGAATTTCCCGATGTTTACCACAAGTATAAAGAGAGCACTATAAAGAAACTAGCAGAAAAAGGTTGCTGGGAAGTTGCAGAAGCAAGGACAAAGAGTAATAGACAGCTTCTTGAATATTTGGTTTACTTAGCAATGGAAGCTGGTTACTCAGAGAAGGTTGATGAGCTATGCGACCGATACTCGCTTGAAGGTTTCTTAAATGGAAAAGTTCCTGAGGCAAGTCTTCTTGAAAATCATTATTTGCATCTCAATGAGTTGGTAGTAGAAGGTGTTGTTTGGGTTGATGAAGTTGATGCCTTGCGCAATGCAACCTCTCACTTTGAGGGATGCAAAGTTGTGGGTCTTGATTGCGAATGGAAACCGAACTATGTAAAAGGCAGCAAACCAAGCAAGGTTTCCATCATGCAAATCGCATCTGAAAAAATGGTTTTCATCTTAGACTTGATCAAGTTATTCAATGACGTTCCTGACATCCTGGATGATTGCGTAACTCGCATTCTGCAATCTCCTAGGATTTTGAAACTCGGCTATAATTTTCAATGTGATATGAAGCAGTTGGCTCATTCGTATGGAGAGTTAGAGTGTTTCAAACACTATGAGATGTTGCTGGATGTCCAGAATGTTTTCAAAGAAGCTCGCGGTGGTCTCTCTGGGCTTGCAAAGAAAATACTGGGAGTTGGTTTGAATAAGACAAGAAGGAATAGCAATTGGGAGCAACGACCTTTGACGCCCAATCAGCTGGAGTATGCAGCTATCGATGCAGCTGTTCTAGTTCACATATTTTGCCATGTTCGGAATCATTCCCAGACTGCCGAGGGGGAAAAACTCGAGTGGAAATCCTGCATCGTTTCCCACATGGATAATAAGAAGACCAAAAAGGGGAGTCGAAAGGAGTCATCAGAAGCTGAGACAAAGGAAGAGCAGCAGCAACTTAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_pol_A_exo1

Protein Analysis

458

Amino Acids

52.46

Weight (kDa)

6.14

Isoelectric Point (pI)

34.43

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 737
AccII CGCG 1 cut(s) 1074
AciI CCGC 2 cut(s) 230, 1074
AcoI YGGCCR 1 cut(s) 268
AcsI RAATTY 1 cut(s) 437
AcyI GRCGYC 1 cut(s) 1163
AfaI GTAC 1 cut(s) 374
AfiI CCNNNNNNNGG 1 cut(s) 1314
AjnI CCWGG 2 cut(s) 366, 909
AjuI GAANNNNNNNTTGG 2 cut(s) 814, 846
AluBI AGCT 8 cut(s) 541, 575, 601, 1070, 1174, 1187, 1199, 1340
AluI AGCT 8 cut(s) 541, 575, 601, 1070, 1174, 1187, 1199, 1340
Alw21I GWGCWC 2 cut(s) 249, 473
Alw26I GTCTC 3 cut(s) 216, 1085, 1337
AlwNI CAGNNNCTG 1 cut(s) 1340
Ama87I CYCGRG 1 cut(s) 1262
AoxI GGCC 1 cut(s) 268
ApeKI GCWGC 8 cut(s) 20, 227, 314, 813, 1184, 1196, 1357, 1360
ApoI RAATTY 1 cut(s) 437
Asp700I GAANNNNTTC 1 cut(s) 1058
AspA2I CCTAGG 1 cut(s) 944
AspLEI GCGC 1 cut(s) 738
AspS9I GGNCC 1 cut(s) 13
AvaI CYCGRG 1 cut(s) 1262
AvaII GGWCC 1 cut(s) 13
AvrII CCTAGG 1 cut(s) 944
AxyI CCTNAGG 1 cut(s) 648
BalI TGGCCA 1 cut(s) 270
BanII GRGCYC 1 cut(s) 53
BbsI GAAGAC 2 cut(s) 650, 1310
Bbv12I GWGCWC 2 cut(s) 249, 473
BbvI GCAGC 7 cut(s) 7, 214, 326, 825, 1196, 1208, 1369
BccI CCATC 2 cut(s) 291, 842
BciT130I CCWGG 2 cut(s) 368, 911
BclI TGATCA 1 cut(s) 879
BcoDI GTCTC 3 cut(s) 216, 1085, 1337
BfaI CTAG 3 cut(s) 485, 945, 1205
BisI GCNGC 8 cut(s) 21, 228, 315, 814, 1185, 1197, 1358, 1361
BlnI CCTAGG 1 cut(s) 944
BlsI GCNGC 8 cut(s) 22, 229, 316, 815, 1186, 1198, 1359, 1362
BmcAI AGTACT 1 cut(s) 374
Bme1390I CCNGG 2 cut(s) 368, 911
Bme18I GGWCC 1 cut(s) 13
BmeT110I CYCGRG 1 cut(s) 1262
BmgT120I GGNCC 1 cut(s) 13
BmrFI CCNGG 2 cut(s) 368, 911
BmrI ACTGGG 1 cut(s) 1115
BmsI GCATC 6 cut(s) 688, 718, 752, 857, 1183, 1287
BmuI ACTGGG 1 cut(s) 1115
BpiI GAAGAC 2 cut(s) 650, 1310
BplI GAGNNNNNCTC 2 cut(s) 236, 268
BpmI CTGGAG 1 cut(s) 1196
Bsa29I ATCGAT 1 cut(s) 1191
BsaHI GRCGYC 1 cut(s) 1163
BsaI GGTCTC 1 cut(s) 1085
BsaJI CCNNGG 2 cut(s) 944, 1248
Bsc4I CCNNNNNNNGG 1 cut(s) 1314
Bse1I ACTGG 2 cut(s) 271, 1110
Bse21I CCTNAGG 1 cut(s) 648
Bse3DI GCAATG 2 cut(s) 573, 745
BseBI CCWGG 2 cut(s) 368, 911
BseCI ATCGAT 1 cut(s) 1191
BseDI CCNNGG 2 cut(s) 944, 1248
BseGI GGATG 4 cut(s) 767, 906, 919, 1051
BseLI CCNNNNNNNGG 1 cut(s) 1314
BseMI GCAATG 2 cut(s) 573, 745
BseMII CTCAG 4 cut(s) 38, 597, 639, 1332
BseNI ACTGG 2 cut(s) 271, 1110
BseXI GCAGC 7 cut(s) 7, 214, 326, 825, 1196, 1208, 1369
BseYI CCCAGC 1 cut(s) 501
Bsh1236I CGCG 1 cut(s) 1074
Bsh1285I CGRYCG 1 cut(s) 610
BshFI GGCC 1 cut(s) 270
BshVI ATCGAT 1 cut(s) 1191
BsiEI CGRYCG 1 cut(s) 610
BsiHKAI GWGCWC 2 cut(s) 249, 473
BsiHKCI CYCGRG 1 cut(s) 1262
BslI CCNNNNNNNGG 1 cut(s) 1314
BsmAI GTCTC 3 cut(s) 216, 1085, 1337
BsmI GAATGC 1 cut(s) 930
BsnI GGCC 1 cut(s) 270
Bso31I GGTCTC 1 cut(s) 1085
BsoBI CYCGRG 1 cut(s) 1262
Bsp1286I GDGCHC 3 cut(s) 53, 249, 473
Bsp143I GATC 1 cut(s) 879
BspACI CCGC 2 cut(s) 230, 1074
BspANI GGCC 1 cut(s) 270
BspCNI CTCAG 4 cut(s) 37, 596, 640, 1333
BspDI ATCGAT 1 cut(s) 1191
BspFNI CGCG 1 cut(s) 1074
BspQI GCTCTTC 1 cut(s) 1347
BspTNI GGTCTC 1 cut(s) 1085
BsrDI GCAATG 2 cut(s) 573, 745
BsrI ACTGG 2 cut(s) 271, 1110
BssECI CCNNGG 2 cut(s) 944, 1248
BssMI GATC 1 cut(s) 879
BssNI GRCGYC 1 cut(s) 1163
BssT1I CCWWGG 1 cut(s) 944
Bst2UI CCWGG 2 cut(s) 368, 911
Bst4CI ACNGT 1 cut(s) 306
Bst6I CTCTTC 1 cut(s) 1347
BstACI GRCGYC 1 cut(s) 1163
BstC8I GCNNGC 2 cut(s) 1072, 1092
BstDEI CTNAG 7 cut(s) 24, 562, 583, 648, 871, 1341, 1367
BstF5I GGATG 4 cut(s) 767, 906, 919, 1051
BstFNI CGCG 1 cut(s) 1074
BstHHI GCGC 1 cut(s) 738
BstKTI GATC 1 cut(s) 882
BstMAI GTCTC 3 cut(s) 216, 1085, 1337
BstMBI GATC 1 cut(s) 879
BstMCI CGRYCG 1 cut(s) 610
BstMWI GCNNNNNNNGC 5 cut(s) 572, 822, 927, 1171, 1193
BstNI CCWGG 2 cut(s) 368, 911
BstNSI RCATGY 1 cut(s) 391
BstSCI CCNGG 2 cut(s) 366, 909
BstUI CGCG 1 cut(s) 1074
BstV1I GCAGC 7 cut(s) 7, 214, 326, 825, 1196, 1208, 1369
BstV2I GAAGAC 2 cut(s) 650, 1310
BstXI CCANNNNNNTGG 2 cut(s) 266, 1174
Bsu15I ATCGAT 1 cut(s) 1191
Bsu36I CCTNAGG 1 cut(s) 648
BsuRI GGCC 1 cut(s) 270
BsuTUI ATCGAT 1 cut(s) 1191
BtsCI GGATG 4 cut(s) 767, 906, 919, 1051
Cac8I GCNNGC 2 cut(s) 1072, 1092
CaiI CAGNNNCTG 1 cut(s) 1340
CfoI GCGC 1 cut(s) 738
Cfr13I GGNCC 1 cut(s) 13
ClaI ATCGAT 1 cut(s) 1191
CseI GACGC 1 cut(s) 1171
Csp6I GTAC 1 cut(s) 373
CviAII CATG 7 cut(s) 62, 337, 388, 434, 838, 1223, 1291
CviQI GTAC 1 cut(s) 373
DdeI CTNAG 7 cut(s) 24, 562, 583, 648, 871, 1341, 1367
DpnI GATC 1 cut(s) 881
DpnII GATC 1 cut(s) 879
EaeI YGGCCR 1 cut(s) 268
Eam1104I CTCTTC 1 cut(s) 1347
EarI CTCTTC 1 cut(s) 1347
Eco130I CCWWGG 1 cut(s) 944
Eco24I GRGCYC 1 cut(s) 53
Eco31I GGTCTC 1 cut(s) 1085
Eco47I GGWCC 1 cut(s) 13
Eco81I CCTNAGG 1 cut(s) 648
Eco88I CYCGRG 1 cut(s) 1262
EcoO109I RGGNCCY 1 cut(s) 13
EcoRII CCWGG 2 cut(s) 366, 909
EcoT14I CCWWGG 1 cut(s) 944
EcoT38I GRGCYC 1 cut(s) 53
ErhI CCWWGG 1 cut(s) 944
FaeI CATG 7 cut(s) 65, 340, 391, 437, 841, 1226, 1294
FatI CATG 7 cut(s) 61, 336, 387, 433, 837, 1222, 1290
FbaI TGATCA 1 cut(s) 879
Fnu4HI GCNGC 8 cut(s) 21, 228, 315, 814, 1185, 1197, 1358, 1361
FokI GGATG 4 cut(s) 774, 893, 926, 1058
FriOI GRGCYC 1 cut(s) 53
Fsp4HI GCNGC 8 cut(s) 21, 228, 315, 814, 1185, 1197, 1358, 1361
FspBI CTAG 3 cut(s) 485, 945, 1205
FspI TGCGCA 1 cut(s) 737
GlaI GCGC 1 cut(s) 737
GluI GCNGC 8 cut(s) 21, 228, 315, 814, 1185, 1197, 1358, 1361
GsaI CCCAGC 1 cut(s) 505
GsuI CTGGAG 1 cut(s) 1196
HaeIII GGCC 1 cut(s) 270
HgaI GACGC 1 cut(s) 1171
HhaI GCGC 1 cut(s) 738
Hin1I GRCGYC 1 cut(s) 1163
Hin1II CATG 7 cut(s) 65, 340, 391, 437, 841, 1226, 1294
Hin6I GCGC 1 cut(s) 736
HinP1I GCGC 1 cut(s) 736
HincII GTYRAC 1 cut(s) 58
HindII GTYRAC 1 cut(s) 58
HinfI GANTC 4 cut(s) 209, 1231, 1318, 1328
Hpy166II GTNNAC 4 cut(s) 58, 451, 559, 1210
Hpy188I TCNGA 5 cut(s) 431, 586, 853, 1230, 1336
Hpy188III TCNNGA 8 cut(s) 35, 443, 545, 647, 662, 779, 902, 1051
Hpy8I GTNNAC 4 cut(s) 58, 451, 559, 1210
HpyAV CCTTC 4 cut(s) 583, 617, 695, 1125
HpyCH4III ACNGT 1 cut(s) 306
HpyCH4IV ACGT 1 cut(s) 897
HpyF10VI GCNNNNNNNGC 5 cut(s) 572, 822, 927, 1171, 1193
HpyF3I CTNAG 7 cut(s) 24, 562, 583, 648, 871, 1341, 1367
HpySE526I ACGT 1 cut(s) 897
Hsp92I GRCGYC 1 cut(s) 1163
Hsp92II CATG 7 cut(s) 65, 340, 391, 437, 841, 1226, 1294
HspAI GCGC 1 cut(s) 736
Ksp22I TGATCA 1 cut(s) 879
Kzo9I GATC 1 cut(s) 879
LguI GCTCTTC 1 cut(s) 1347
LmnI GCTCC 1 cut(s) 1147
Lsp1109I GCAGC 7 cut(s) 7, 214, 326, 825, 1196, 1208, 1369
LweI GCATC 6 cut(s) 688, 718, 752, 857, 1183, 1287
MaeI CTAG 3 cut(s) 485, 945, 1205
MaeII ACGT 1 cut(s) 897
MaeIII GTNAC 3 cut(s) 232, 578, 922
MalI GATC 1 cut(s) 881
MboI GATC 1 cut(s) 879
MboII GAAGA 4 cut(s) 94, 650, 1315, 1364
MfeI CAATTG 1 cut(s) 1141
MhlI GDGCHC 3 cut(s) 53, 249, 473
MlsI TGGCCA 1 cut(s) 270
MluCI AATT 4 cut(s) 151, 437, 967, 1141
MluNI TGGCCA 1 cut(s) 270
MlyI GAGTC 3 cut(s) 218, 1327, 1337
MmeI TCCRAC 1 cut(s) 454
MnlI CCTC 6 cut(s) 26, 63, 643, 751, 757, 1243
Mox20I TGGCCA 1 cut(s) 270
MroXI GAANNNNTTC 1 cut(s) 1058
MscI TGGCCA 1 cut(s) 270
MseI TTAA 1 cut(s) 632
MslI CAYNNNNRTG 2 cut(s) 684, 1002
Msp20I TGGCCA 1 cut(s) 270
MspA1I CMGCKG 2 cut(s) 1174, 1199
MspR9I CCNGG 2 cut(s) 368, 911
MunI CAATTG 1 cut(s) 1141
Mva1269I GAATGC 1 cut(s) 930
MvaI CCWGG 2 cut(s) 368, 911
MvnI CGCG 1 cut(s) 1074
MwoI GCNNNNNNNGC 5 cut(s) 572, 822, 927, 1171, 1193
NdeII GATC 1 cut(s) 879
NlaIII CATG 7 cut(s) 65, 340, 391, 437, 841, 1226, 1294
NmeAIII GCCGAG 2 cut(s) 939, 1273
NsbI TGCGCA 1 cut(s) 737
NspI RCATGY 1 cut(s) 391
PaeR7I CTCGAG 1 cut(s) 1262
PciSI GCTCTTC 1 cut(s) 1347
PctI GAATGC 1 cut(s) 930
PdmI GAANNNNTTC 1 cut(s) 1058
PfeI GAWTC 1 cut(s) 1231
PfoI TCCNGGA 2 cut(s) 366, 909
PkrI GCNGC 8 cut(s) 22, 229, 316, 815, 1186, 1198, 1359, 1362
PleI GAGTC 3 cut(s) 217, 1326, 1336
PpsI GAGTC 3 cut(s) 217, 1326, 1336
PpuMI RGGWCCY 1 cut(s) 13
Psp5II RGGWCCY 1 cut(s) 13
Psp6I CCWGG 2 cut(s) 366, 909
PspFI CCCAGC 1 cut(s) 501
PspGI CCWGG 2 cut(s) 366, 909
PspPI GGNCC 1 cut(s) 13
PspPPI RGGWCCY 1 cut(s) 13
PspXI VCTCGAGB 1 cut(s) 1262
PstNI CAGNNNCTG 1 cut(s) 1340
PvuII CAGCTG 2 cut(s) 1174, 1199
RsaI GTAC 1 cut(s) 374
RsaNI GTAC 1 cut(s) 373
RseI CAYNNNNRTG 2 cut(s) 684, 1002
SapI GCTCTTC 1 cut(s) 1347
SaqAI TTAA 1 cut(s) 632
SatI GCNGC 8 cut(s) 21, 228, 315, 814, 1185, 1197, 1358, 1361
Sau3AI GATC 1 cut(s) 879
Sau96I GGNCC 1 cut(s) 13
ScaI AGTACT 1 cut(s) 374
SchI GAGTC 3 cut(s) 218, 1327, 1337
ScrFI CCNGG 2 cut(s) 368, 911
SduI GDGCHC 3 cut(s) 53, 249, 473
SfaNI GCATC 6 cut(s) 688, 718, 752, 857, 1183, 1287
Sfr274I CTCGAG 1 cut(s) 1262
SinI GGWCC 1 cut(s) 13
SlaI CTCGAG 1 cut(s) 1262
SmiMI CAYNNNNRTG 2 cut(s) 684, 1002
SmlI CTYRAG 1 cut(s) 1262
SmoI CTYRAG 1 cut(s) 1262
Sse9I AATT 4 cut(s) 151, 437, 967, 1141
SsiI CCGC 2 cut(s) 230, 1074
SspI AATATT 1 cut(s) 551
SspMI CTAG 3 cut(s) 485, 945, 1205
StyD4I CCNGG 2 cut(s) 366, 909
StyI CCWWGG 1 cut(s) 944
TaaI ACNGT 1 cut(s) 306
TaiI ACGT 1 cut(s) 900
TaqI TCGA 4 cut(s) 36, 1191, 1263, 1321
TaqII GACCGA 1 cut(s) 624
TasI AATT 4 cut(s) 151, 437, 967, 1141
TatI WGTACW 1 cut(s) 372
TfiI GAWTC 1 cut(s) 1231
Tru1I TTAA 1 cut(s) 632
Tru9I TTAA 1 cut(s) 632
TseI GCWGC 8 cut(s) 20, 227, 314, 813, 1184, 1196, 1357, 1360
TspDTI ATGAA 5 cut(s) 325, 450, 735, 856, 998
VpaK11BI GGWCC 1 cut(s) 13
XapI RAATTY 1 cut(s) 437
XceI RCATGY 1 cut(s) 391
XcmI CCANNNNNNNNNTGG 1 cut(s) 374
XhoI CTCGAG 1 cut(s) 1262
XmaJI CCTAGG 1 cut(s) 944
XmnI GAANNNNTTC 1 cut(s) 1058
XspI CTAG 3 cut(s) 485, 945, 1205
ZrmI AGTACT 1 cut(s) 374
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.