RLG00000025277

3'-5' exonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0003918
Physical Location & Seq
Reverse (-)
43542039 .. 43544608
2570 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025277

Sequence Viewer

Length: 1215 bp
ATGGCAACAAAATTTGTTGAGGATTATATATATCAGTTGATAGAGTCTCAAACATACATGACTGCGGTTACTCTATTGACGCACTTCTCTATCCGCCAGTCTGGTGAATCTTTTCTGCTTTGTATGATAGAAAGCCAACAATTGAAAGCAGCAGAGAAGTGGGCAACATTCATCGGAAAGCCAGTGTTATGTGTTCTGGTCCAAGAGTATGTTGATAGAAACATGCTAAAGAATGCTTACGAGATTATACACAAAAACAATCTCCGACAGGAATTCCCCGACGTTCATCACAAGTATAAAGAGAGCTCATTAAAGAAACTAGCAGAAAAAGGATGCTGGGATGTGGCAGAAGCAAAGACAAAGAGTAATAGACAACTCCTTGAATACCTGGTTTATTTAGCCATGGAAGCTGGTTACTCAGAAAAGGTTGATGAACTATGTGTTCGATATTCACTTAAAGGTTTCTTGAATGCCAAAGTGCCTGAGCCAAGCCTTCTCCATAGTCGCTATTTGCATCTGGATGAGTTAGTTGTAGAAGCTGTCATTTGGGTTGATGAAGTTATTGCTTTGCGTAAGGCAACCTGTCATCTTGAGGAATGCAAAGTTGTTGGTCTTGATTGTGAATGGAAACCGAATTATGTAAAAGGTAGCAGACCAAACAAGGTTTCTATTATGCAAGTTGCTTCTGATAAGATGGTTTTCATACTTGACTTGATCAAATTATATGAAGACGTGCCTGATGTTCTGGATGACAGCTTAACTCGTATTCTACAGTCTCCTAGGATCCTAAAACTTGGCTATAATTTTCAATGTGATATGAGGCAGCTGGCTCAGTCATATGAAGAGTTAGAGTGTTTCAAGCACTATCAGATGCTACTGGACATTCAGAATGTGTTCAAAGAACCTCGGGGTGGTCTATCCGGGCTTGCAAAGAAAATACTTGGAGTTGGTTTGAACAAGACAAGAAGGAACAGCAATTGGGAGCAACGGCCTCTAACACCCAATCAGCTGGAATATGCAGCTCTCGATGCAGCTGTACTGGTTCACATATTTGGCCACGTTCGCGGTCATTCTCAGCCTGCTGATGGAGAGGGACATGGAAAACTCGAGTGGAAATCCTGCATTGTTTCCGACATGAATAACATCAAGAAGTCCAAGAAGGGAAGTCGAAAGGAGTCTTCTGAAGCTCAGACAAAGGAGCAGTTAGCAATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_pol_A_exo1

Protein Analysis

405

Amino Acids

46.35

Weight (kDa)

7.56

Isoelectric Point (pI)

42.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_pol_A_exo1 PF01612 182 - 353 4.3e-22 3'-5' exonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 1065
AciI CCGC 3 cut(s) 65, 94, 1065
AclWI GGATC 2 cut(s) 778, 791
AcoI YGGCCR 1 cut(s) 1054
AcsI RAATTY 2 cut(s) 11, 272
AcuI CTGAAG 1 cut(s) 1203
AfaI GTAC 1 cut(s) 1038
AfiI CCNNNNNNNGG 2 cut(s) 911, 1085
AgsI TTSAA 7 cut(s) 145, 383, 469, 809, 859, 898, 955
AjiI CACGTC 1 cut(s) 733
AjnI CCWGG 1 cut(s) 387
AjuI GAANNNNNNNTTGG 2 cut(s) 649, 681
AluBI AGCT 9 cut(s) 306, 410, 539, 756, 826, 1009, 1022, 1034, 1187
AluI AGCT 9 cut(s) 306, 410, 539, 756, 826, 1009, 1022, 1034, 1187
Alw21I GWGCWC 1 cut(s) 308
Alw26I GTCTC 2 cut(s) 51, 780
AlwI GGATC 2 cut(s) 778, 791
Ama87I CYCGRG 2 cut(s) 906, 1106
AoxI GGCC 2 cut(s) 989, 1054
ApeKI GCWGC 4 cut(s) 149, 823, 1019, 1031
ApoI RAATTY 2 cut(s) 11, 272
Asp700I GAANNNNTTC 2 cut(s) 111, 893
AspA2I CCTAGG 1 cut(s) 779
AspS9I GGNCC 1 cut(s) 199
AsuC2I CCSGG 1 cut(s) 922
AsuHPI GGTGA 1 cut(s) 116
AvaI CYCGRG 2 cut(s) 906, 1106
AvaII GGWCC 1 cut(s) 199
AvrII CCTAGG 1 cut(s) 779
BalI TGGCCA 1 cut(s) 1056
BamHI GGATCC 1 cut(s) 783
BanII GRGCYC 1 cut(s) 308
BbsI GAAGAC 2 cut(s) 735, 1170
Bbv12I GWGCWC 1 cut(s) 308
BbvI GCAGC 4 cut(s) 161, 835, 1031, 1043
BccI CCATC 2 cut(s) 688, 1079
BceAI ACGGC 1 cut(s) 1004
BciT130I CCWGG 1 cut(s) 389
BclI TGATCA 1 cut(s) 714
BcnI CCSGG 1 cut(s) 922
BcoDI GTCTC 2 cut(s) 51, 780
BfaI CTAG 2 cut(s) 320, 780
BfmI CTRYAG 1 cut(s) 770
BisI GCNGC 4 cut(s) 150, 824, 1020, 1032
BlnI CCTAGG 1 cut(s) 779
BlsI GCNGC 4 cut(s) 151, 825, 1021, 1033
Bme1390I CCNGG 2 cut(s) 389, 922
Bme18I GGWCC 1 cut(s) 199
BmeT110I CYCGRG 2 cut(s) 906, 1106
BmgBI CACGTC 1 cut(s) 733
BmgT120I GGNCC 1 cut(s) 199
BmiI GGNNCC 1 cut(s) 785
BmrFI CCNGG 2 cut(s) 389, 922
BmsI GCATC 4 cut(s) 323, 523, 861, 1018
BpiI GAAGAC 2 cut(s) 735, 1170
Bpu10I CCTNAGC 1 cut(s) 483
BpuEI CTTGAG 1 cut(s) 611
BpuMI CCSGG 1 cut(s) 922
BsaJI CCNNGG 3 cut(s) 402, 779, 905
Bsc4I CCNNNNNNNGG 2 cut(s) 911, 1085
Bse1I ACTGG 4 cut(s) 97, 182, 882, 1044
BseBI CCWGG 1 cut(s) 389
BseDI CCNNGG 3 cut(s) 402, 779, 905
BseGI GGATG 4 cut(s) 338, 346, 526, 754
BseLI CCNNNNNNNGG 2 cut(s) 911, 1085
BseMII CTCAG 5 cut(s) 432, 474, 845, 1088, 1202
BseNI ACTGG 4 cut(s) 97, 182, 882, 1044
BseXI GCAGC 4 cut(s) 161, 835, 1031, 1043
BseYI CCCAGC 1 cut(s) 336
Bsh1236I CGCG 1 cut(s) 1065
BshFI GGCC 2 cut(s) 991, 1056
BsiHKAI GWGCWC 1 cut(s) 308
BsiHKCI CYCGRG 2 cut(s) 906, 1106
BsiSI CCGG 1 cut(s) 921
BslFI GGGAC 1 cut(s) 1107
BslI CCNNNNNNNGG 2 cut(s) 911, 1085
BsmAI GTCTC 2 cut(s) 51, 780
BsmFI GGGAC 1 cut(s) 1107
BsmI GAATGC 3 cut(s) 238, 475, 602
BsnI GGCC 2 cut(s) 991, 1056
BsoBI CYCGRG 2 cut(s) 906, 1106
Bsp1286I GDGCHC 1 cut(s) 308
Bsp143I GATC 2 cut(s) 714, 783
Bsp19I CCATGG 1 cut(s) 402
BspACI CCGC 3 cut(s) 65, 94, 1065
BspANI GGCC 2 cut(s) 991, 1056
BspCNI CTCAG 5 cut(s) 431, 475, 844, 1087, 1201
BspFNI CGCG 1 cut(s) 1065
BspLI GGNNCC 1 cut(s) 785
BspPI GGATC 2 cut(s) 778, 791
BsrI ACTGG 4 cut(s) 97, 182, 882, 1044
BssECI CCNNGG 3 cut(s) 402, 779, 905
BssMI GATC 2 cut(s) 714, 783
BssT1I CCWWGG 2 cut(s) 402, 779
Bst2UI CCWGG 1 cut(s) 389
Bst4CI ACNGT 1 cut(s) 774
Bst6I CTCTTC 1 cut(s) 837
BstC8I GCNNGC 3 cut(s) 828, 927, 1080
BstDEI CTNAG 5 cut(s) 418, 483, 831, 1074, 1188
BstDSI CCRYGG 1 cut(s) 402
BstF5I GGATG 4 cut(s) 338, 346, 526, 754
BstFNI CGCG 1 cut(s) 1065
BstKTI GATC 2 cut(s) 717, 786
BstMAI GTCTC 2 cut(s) 51, 780
BstMBI GATC 2 cut(s) 714, 783
BstMWI GCNNNNNNNGC 3 cut(s) 407, 1028, 1062
BstNI CCWGG 1 cut(s) 389
BstNSI RCATGY 1 cut(s) 226
BstSCI CCNGG 2 cut(s) 387, 920
BstSFI CTRYAG 1 cut(s) 770
BstUI CGCG 1 cut(s) 1065
BstV1I GCAGC 4 cut(s) 161, 835, 1031, 1043
BstV2I GAAGAC 2 cut(s) 735, 1170
BstX2I RGATCY 1 cut(s) 783
BstXI CCANNNNNNTGG 1 cut(s) 1009
BstYI RGATCY 1 cut(s) 783
BsuRI GGCC 2 cut(s) 991, 1056
BtgI CCRYGG 1 cut(s) 402
BtrI CACGTC 1 cut(s) 733
BtsCI GGATG 4 cut(s) 338, 346, 526, 754
BtsIMutI CAGTG 1 cut(s) 189
Cac8I GCNNGC 3 cut(s) 828, 927, 1080
Cfr13I GGNCC 1 cut(s) 199
CseI GACGC 1 cut(s) 88
CsiI ACCWGGT 1 cut(s) 387
Csp6I GTAC 1 cut(s) 1037
CviAII CATG 5 cut(s) 58, 223, 403, 1097, 1135
CviQI GTAC 1 cut(s) 1037
DdeI CTNAG 5 cut(s) 418, 483, 831, 1074, 1188
DpnI GATC 2 cut(s) 716, 785
DpnII GATC 2 cut(s) 714, 783
EaeI YGGCCR 1 cut(s) 1054
Eam1104I CTCTTC 1 cut(s) 837
EarI CTCTTC 1 cut(s) 837
EciI GGCGGA 1 cut(s) 83
Ecl136II GAGCTC 1 cut(s) 306
Eco130I CCWWGG 2 cut(s) 402, 779
Eco24I GRGCYC 1 cut(s) 308
Eco47I GGWCC 1 cut(s) 199
Eco53kI GAGCTC 1 cut(s) 306
Eco57I CTGAAG 1 cut(s) 1203
Eco88I CYCGRG 2 cut(s) 906, 1106
EcoICRI GAGCTC 1 cut(s) 306
EcoRI GAATTC 1 cut(s) 272
EcoRII CCWGG 1 cut(s) 387
EcoT14I CCWWGG 2 cut(s) 402, 779
EcoT38I GRGCYC 1 cut(s) 308
ErhI CCWWGG 2 cut(s) 402, 779
FaeI CATG 5 cut(s) 61, 226, 406, 1100, 1138
FaqI GGGAC 1 cut(s) 1107
FatI CATG 5 cut(s) 57, 222, 402, 1096, 1134
FauNDI CATATG 1 cut(s) 838
FbaI TGATCA 1 cut(s) 714
Fnu4HI GCNGC 4 cut(s) 150, 824, 1020, 1032
FokI GGATG 4 cut(s) 345, 353, 533, 761
FriOI GRGCYC 1 cut(s) 308
Fsp4HI GCNGC 4 cut(s) 150, 824, 1020, 1032
FspBI CTAG 2 cut(s) 320, 780
GluI GCNGC 4 cut(s) 150, 824, 1020, 1032
GsaI CCCAGC 1 cut(s) 340
HaeIII GGCC 2 cut(s) 991, 1056
HapII CCGG 1 cut(s) 921
HgaI GACGC 1 cut(s) 88
Hin1II CATG 5 cut(s) 61, 226, 406, 1100, 1138
HinfI GANTC 3 cut(s) 44, 107, 1175
HpaII CCGG 1 cut(s) 921
HphI GGTGA 1 cut(s) 116
Hpy166II GTNNAC 1 cut(s) 1045
Hpy188I TCNGA 9 cut(s) 176, 266, 421, 688, 870, 888, 1132, 1183, 1191
Hpy188III TCNNGA 7 cut(s) 466, 518, 590, 614, 746, 1025, 1147
Hpy8I GTNNAC 1 cut(s) 1045
Hpy99I CGWCG 1 cut(s) 284
HpyAV CCTTC 3 cut(s) 503, 960, 1153
HpyCH4III ACNGT 1 cut(s) 774
HpyCH4IV ACGT 3 cut(s) 282, 732, 1059
HpyCH4V TGCA 7 cut(s) 514, 600, 676, 929, 1019, 1031, 1122
HpyF10VI GCNNNNNNNGC 3 cut(s) 407, 1028, 1062
HpyF3I CTNAG 5 cut(s) 418, 483, 831, 1074, 1188
HpySE526I ACGT 3 cut(s) 282, 732, 1059
Hsp92II CATG 5 cut(s) 61, 226, 406, 1100, 1138
Ksp22I TGATCA 1 cut(s) 714
Kzo9I GATC 2 cut(s) 714, 783
LmnI GCTCC 2 cut(s) 982, 1198
Lsp1109I GCAGC 4 cut(s) 161, 835, 1031, 1043
LweI GCATC 4 cut(s) 323, 523, 861, 1018
MabI ACCWGGT 1 cut(s) 387
MaeI CTAG 2 cut(s) 320, 780
MaeII ACGT 3 cut(s) 282, 732, 1059
MaeIII GTNAC 2 cut(s) 67, 413
MalI GATC 2 cut(s) 716, 785
MboI GATC 2 cut(s) 714, 783
MboII GAAGA 3 cut(s) 740, 854, 1170
MfeI CAATTG 2 cut(s) 140, 976
MflI RGATCY 1 cut(s) 783
MhlI GDGCHC 1 cut(s) 308
MlsI TGGCCA 1 cut(s) 1056
MluCI AATT 8 cut(s) 11, 140, 272, 634, 719, 802, 976, 1209
MluNI TGGCCA 1 cut(s) 1056
MlyI GAGTC 2 cut(s) 53, 1184
MmeI TCCRAC 2 cut(s) 289, 1155
MnlI CCTC 6 cut(s) 13, 586, 813, 915, 1002, 1084
Mox20I TGGCCA 1 cut(s) 1056
MroXI GAANNNNTTC 2 cut(s) 111, 893
MscI TGGCCA 1 cut(s) 1056
MseI TTAA 3 cut(s) 311, 456, 758
MslI CAYNNNNRTG 1 cut(s) 519
Msp20I TGGCCA 1 cut(s) 1056
MspA1I CMGCKG 3 cut(s) 826, 1009, 1034
MspI CCGG 1 cut(s) 921
MspR9I CCNGG 2 cut(s) 389, 922
MunI CAATTG 2 cut(s) 140, 976
Mva1269I GAATGC 3 cut(s) 238, 475, 602
MvaI CCWGG 1 cut(s) 389
MvnI CGCG 1 cut(s) 1065
MwoI GCNNNNNNNGC 3 cut(s) 407, 1028, 1062
NciI CCSGG 1 cut(s) 922
NcoI CCATGG 1 cut(s) 402
NdeI CATATG 1 cut(s) 838
NdeII GATC 2 cut(s) 714, 783
NlaIII CATG 5 cut(s) 61, 226, 406, 1100, 1138
NlaIV GGNNCC 1 cut(s) 785
NspI RCATGY 1 cut(s) 226
PaeR7I CTCGAG 1 cut(s) 1106
PctI GAATGC 3 cut(s) 238, 475, 602
PdmI GAANNNNTTC 2 cut(s) 111, 893
PfeI GAWTC 1 cut(s) 107
PkrI GCNGC 4 cut(s) 151, 825, 1021, 1033
PleI GAGTC 2 cut(s) 52, 1183
PpsI GAGTC 2 cut(s) 52, 1183
Psp124BI GAGCTC 1 cut(s) 308
Psp6I CCWGG 1 cut(s) 387
PspFI CCCAGC 1 cut(s) 336
PspGI CCWGG 1 cut(s) 387
PspN4I GGNNCC 1 cut(s) 785
PspPI GGNCC 1 cut(s) 199
PspXI VCTCGAGB 1 cut(s) 1106
PsuI RGATCY 1 cut(s) 783
PvuII CAGCTG 3 cut(s) 826, 1009, 1034
RsaI GTAC 1 cut(s) 1038
RsaNI GTAC 1 cut(s) 1037
RseI CAYNNNNRTG 1 cut(s) 519
SacI GAGCTC 1 cut(s) 308
SaqAI TTAA 3 cut(s) 311, 456, 758
SatI GCNGC 4 cut(s) 150, 824, 1020, 1032
Sau3AI GATC 2 cut(s) 714, 783
Sau96I GGNCC 1 cut(s) 199
SchI GAGTC 2 cut(s) 53, 1184
ScrFI CCNGG 2 cut(s) 389, 922
SduI GDGCHC 1 cut(s) 308
SexAI ACCWGGT 1 cut(s) 387
SfaNI GCATC 4 cut(s) 323, 523, 861, 1018
SfcI CTRYAG 1 cut(s) 770
Sfr274I CTCGAG 1 cut(s) 1106
SinI GGWCC 1 cut(s) 199
SlaI CTCGAG 1 cut(s) 1106
SmiMI CAYNNNNRTG 1 cut(s) 519
SmlI CTYRAG 2 cut(s) 590, 1106
SmoI CTYRAG 2 cut(s) 590, 1106
Sse9I AATT 8 cut(s) 11, 140, 272, 634, 719, 802, 976, 1209
SsiI CCGC 3 cut(s) 65, 94, 1065
SspMI CTAG 2 cut(s) 320, 780
SstI GAGCTC 1 cut(s) 308
StyD4I CCNGG 2 cut(s) 387, 920
StyI CCWWGG 2 cut(s) 402, 779
TaaI ACNGT 1 cut(s) 774
TaiI ACGT 3 cut(s) 285, 735, 1062
TaqI TCGA 4 cut(s) 445, 1026, 1107, 1168
TasI AATT 8 cut(s) 11, 140, 272, 634, 719, 802, 976, 1209
TatI WGTACW 1 cut(s) 1036
TfiI GAWTC 1 cut(s) 107
Tru1I TTAA 3 cut(s) 311, 456, 758
Tru9I TTAA 3 cut(s) 311, 456, 758
TscAI CASTG 1 cut(s) 189
TseI GCWGC 4 cut(s) 149, 823, 1019, 1031
TspDTI ATGAA 8 cut(s) 160, 275, 447, 570, 691, 741, 855, 1151
TspRI CASTG 1 cut(s) 189
VpaK11BI GGWCC 1 cut(s) 199
XapI RAATTY 2 cut(s) 11, 272
XceI RCATGY 1 cut(s) 226
XhoI CTCGAG 1 cut(s) 1106
XmaJI CCTAGG 1 cut(s) 779
XmnI GAANNNNTTC 2 cut(s) 111, 893
XspI CTAG 2 cut(s) 320, 780
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.