Rorug07G0293000

3'-5' exonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0003918
Physical Location & Seq
Forward (+)
28626668 .. 28627120
453 bp
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UTR
Exon/CDS
Intron
Rorug07G0293000.1

Sequence Viewer

Length: 453 bp
ATGGTAGTTTATTTTCTGTTTTCAATAAATCCCGACCAACTTTTGGTTTGGAGGTGTGGGCATTGTCCCGGTCTGCGCACCTTGTGTGCCATGTCTGCTCTAGGTAGACGGCAAGTTCCTTGCTTTGTCAAATGGTCGCAGCCTCCTAGTGGCAGAATGAAACCAAGTGCTACCGGAGTGTTTTTCCGGTGGCAATATAGTGGGAAAGCTGATAGTATTAGTAACTTATGGCTCCGCGTGACCATTGTCTTTCCGCTATGTCACCATATTTGTAAAGCAAATAGAGTAGCCTATAGAGCACTCTTCGCTAATTGGTGCATGTTAGAATGCGTATTTTTAGTTGAGACACATGTTATTATCTCGGGTGATTCTCTTAGTGCTTATTGTAATTTGGGGTTTAGGCAATATGTCCCCCCCATGTATTCTTTGGTTTCATTAATGGTTAAGGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_pol_A_exo1

Protein Analysis

150

Amino Acids

17.09

Weight (kDa)

9.44

Isoelectric Point (pI)

45.48

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 77
AccB7I CCANNNNNTGG 1 cut(s) 43
AccI GTMKAC 1 cut(s) 106
AccII CGCG 1 cut(s) 237
AciI CCGC 2 cut(s) 235, 254
AdeI CACNNNGTG 1 cut(s) 84
AfiI CCNNNNNNNGG 2 cut(s) 43, 149
AflIII ACRYGT 1 cut(s) 349
AgsI TTSAA 1 cut(s) 24
AluBI AGCT 1 cut(s) 209
AluI AGCT 1 cut(s) 209
Alw21I GWGCWC 1 cut(s) 301
Alw26I GTCTC 1 cut(s) 338
Ama87I CYCGRG 1 cut(s) 361
ApeKI GCWGC 1 cut(s) 139
AseI ATTAAT 1 cut(s) 437
AspLEI GCGC 1 cut(s) 78
AsuC2I CCSGG 1 cut(s) 69
AsuHPI GGTGA 2 cut(s) 254, 377
AvaI CYCGRG 1 cut(s) 361
Bbv12I GWGCWC 1 cut(s) 301
BbvI GCAGC 1 cut(s) 151
BceAI ACGGC 1 cut(s) 125
BcnI CCSGG 1 cut(s) 69
BcoDI GTCTC 1 cut(s) 338
BfaI CTAG 2 cut(s) 101, 147
BfmI CTRYAG 1 cut(s) 292
BisI GCNGC 1 cut(s) 140
BlsI GCNGC 1 cut(s) 141
Bme1390I CCNGG 1 cut(s) 69
BmeT110I CYCGRG 1 cut(s) 361
BmiI GGNNCC 1 cut(s) 233
BmrFI CCNGG 1 cut(s) 69
BoxI GACNNNNGTC 1 cut(s) 245
BpuMI CCSGG 1 cut(s) 69
BsaWI WCCGGW 2 cut(s) 173, 186
Bsc4I CCNNNNNNNGG 2 cut(s) 43, 149
BseLI CCNNNNNNNGG 2 cut(s) 43, 149
BseXI GCAGC 1 cut(s) 151
Bsh1236I CGCG 1 cut(s) 237
BsiHKAI GWGCWC 1 cut(s) 301
BsiHKCI CYCGRG 1 cut(s) 361
BsiSI CCGG 3 cut(s) 69, 174, 187
BslFI GGGAC 2 cut(s) 51, 395
BslI CCNNNNNNNGG 2 cut(s) 43, 149
BsmAI GTCTC 1 cut(s) 338
BsmFI GGGAC 2 cut(s) 51, 395
BsmI GAATGC 1 cut(s) 332
BsoBI CYCGRG 1 cut(s) 361
Bsp1286I GDGCHC 1 cut(s) 301
BspACI CCGC 2 cut(s) 235, 254
BspFNI CGCG 1 cut(s) 237
BspLI GGNNCC 1 cut(s) 233
Bst6I CTCTTC 1 cut(s) 308
BstDEI CTNAG 1 cut(s) 374
BstFNI CGCG 1 cut(s) 237
BstHHI GCGC 1 cut(s) 78
BstMAI GTCTC 1 cut(s) 338
BstMWI GCNNNNNNNGC 3 cut(s) 95, 296, 305
BstNSI RCATGY 2 cut(s) 322, 353
BstPAI GACNNNNGTC 1 cut(s) 245
BstSCI CCNGG 1 cut(s) 67
BstSFI CTRYAG 1 cut(s) 292
BstUI CGCG 1 cut(s) 237
BstV1I GCAGC 1 cut(s) 151
CfoI GCGC 1 cut(s) 78
CviAII CATG 4 cut(s) 91, 319, 350, 418
CviJI RGCY 5 cut(s) 142, 209, 232, 290, 449
CviKI_1 RGCY 5 cut(s) 142, 209, 232, 290, 449
DdeI CTNAG 1 cut(s) 374
DraIII CACNNNGTG 1 cut(s) 84
Eam1104I CTCTTC 1 cut(s) 308
EarI CTCTTC 1 cut(s) 308
Eco88I CYCGRG 1 cut(s) 361
FaeI CATG 4 cut(s) 94, 322, 353, 421
FaqI GGGAC 2 cut(s) 51, 395
FatI CATG 4 cut(s) 90, 318, 349, 417
FblI GTMKAC 1 cut(s) 106
Fnu4HI GCNGC 1 cut(s) 140
Fsp4HI GCNGC 1 cut(s) 140
FspBI CTAG 2 cut(s) 101, 147
FspI TGCGCA 1 cut(s) 77
GlaI GCGC 1 cut(s) 77
GluI GCNGC 1 cut(s) 140
HapII CCGG 3 cut(s) 69, 174, 187
HhaI GCGC 1 cut(s) 78
Hin1II CATG 4 cut(s) 94, 322, 353, 421
Hin6I GCGC 1 cut(s) 76
HinP1I GCGC 1 cut(s) 76
HinfI GANTC 1 cut(s) 368
HpaII CCGG 3 cut(s) 69, 174, 187
HphI GGTGA 2 cut(s) 254, 377
Hpy166II GTNNAC 1 cut(s) 107
Hpy188III TCNNGA 1 cut(s) 32
Hpy8I GTNNAC 1 cut(s) 107
HpyCH4V TGCA 1 cut(s) 318
HpyF10VI GCNNNNNNNGC 3 cut(s) 95, 296, 305
HpyF3I CTNAG 1 cut(s) 374
Hsp92II CATG 4 cut(s) 94, 322, 353, 421
HspAI GCGC 1 cut(s) 76
LmnI GCTCC 1 cut(s) 237
LpnPI CCDG 3 cut(s) 82, 187, 200
Lsp1109I GCAGC 1 cut(s) 151
MaeI CTAG 2 cut(s) 101, 147
MaeIII GTNAC 3 cut(s) 221, 238, 260
MboII GAAGA 1 cut(s) 295
MhlI GDGCHC 1 cut(s) 301
MluCI AATT 2 cut(s) 310, 388
MnlI CCTC 2 cut(s) 45, 153
MseI TTAA 3 cut(s) 437, 444, 451
MspI CCGG 3 cut(s) 69, 174, 187
MspR9I CCNGG 1 cut(s) 69
Mva1269I GAATGC 1 cut(s) 332
MvnI CGCG 1 cut(s) 237
MwoI GCNNNNNNNGC 3 cut(s) 95, 296, 305
NciI CCSGG 1 cut(s) 69
NlaIII CATG 4 cut(s) 94, 322, 353, 421
NlaIV GGNNCC 1 cut(s) 233
NmuCI GTSAC 2 cut(s) 238, 260
NsbI TGCGCA 1 cut(s) 77
NspI RCATGY 2 cut(s) 322, 353
PciI ACATGT 1 cut(s) 349
PctI GAATGC 1 cut(s) 332
PfeI GAWTC 1 cut(s) 368
PflMI CCANNNNNTGG 1 cut(s) 43
PkrI GCNGC 1 cut(s) 141
PscI ACATGT 1 cut(s) 349
PshAI GACNNNNGTC 1 cut(s) 245
PshBI ATTAAT 1 cut(s) 437
PspN4I GGNNCC 1 cut(s) 233
SaqAI TTAA 3 cut(s) 437, 444, 451
SatI GCNGC 1 cut(s) 140
ScrFI CCNGG 1 cut(s) 69
SduI GDGCHC 1 cut(s) 301
SetI ASST 4 cut(s) 56, 83, 106, 211
SfcI CTRYAG 1 cut(s) 292
Sse9I AATT 2 cut(s) 310, 388
SsiI CCGC 2 cut(s) 235, 254
SspMI CTAG 2 cut(s) 101, 147
StyD4I CCNGG 1 cut(s) 67
TasI AATT 2 cut(s) 310, 388
TfiI GAWTC 1 cut(s) 368
Tru1I TTAA 3 cut(s) 437, 444, 451
Tru9I TTAA 3 cut(s) 437, 444, 451
TseFI GTSAC 2 cut(s) 238, 260
TseI GCWGC 1 cut(s) 139
Tsp45I GTSAC 2 cut(s) 238, 260
TspDTI ATGAA 2 cut(s) 173, 423
Van91I CCANNNNNTGG 1 cut(s) 43
VspI ATTAAT 1 cut(s) 437
XceI RCATGY 2 cut(s) 322, 353
XcmI CCANNNNNNNNNTGG 1 cut(s) 424
XmiI GTMKAC 1 cut(s) 106
XspI CTAG 2 cut(s) 101, 147
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.