Rorug03G0015800

3'-5' exonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0003918
Physical Location & Seq
Reverse (-)
1271720 .. 1272759
1040 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0015800.1

Sequence Viewer

Length: 960 bp
ATGGTGGTGGCAGCCATCTGGTATTATGTGAAGAAATCAGACAGGATAGAGGAAAAGGAAAATCAAATCAAGATTGAAAAGTTCCTAGATGATCACAAATCCCATATGACAAATGGATTCAGAAAGAAGTTGGGTGAAGGAGGTTTTGGAAGTGTTTTCAGTGGAAAGCTTCCAAGTAATGGAGTTCCGGTAGCCATAAAAGTCCTCAAGGATTCTAAAGGAAATGGAGATGATTTTGTTAATGAAGTGGGAACCATTGGTAGGATTCACCACATAAACGTGGTTCGCCTACTTGGGTTTTCTGCTGAAGGAGGCAAGCGTGCTCTTATTTATGAAGACATGCCAAACAGCTCTCTGGAGAAGTTTATCACATCTAAAGATCAATCTAGTGATAACACTGCTTCATTTGATTGGATGAAACTTCGCAACATCATCACAGGTATAGCAAAGGGAATCGAGTATCTACACCAAGGGTGCGACCAAAGGATCCTCCACTTCGATATCAAGCCTCATAATATCTTGTTAGACCATGACTTCAATCCGAAGATCTCCGATTTTGGCCTGGCCAAACTCTGCTCCGAAGACAGTCTTATATCTACGACTGCTGCCAGAGGCACCATAGGCTACATAGCACCTGAAGTGTTTAATGGAAACTTTGGAACCATCTCCTACAAATCAGATGTGTACAGTTTTGGGATGCTAGTGCTTGAAGTTATTGGAGCTAGGAAGGAATCCACTGTTACATCGGCCAACAATAGAGGAGAAGAGCTGGATTTGGAGCTAGGTGACGATGGAGATGCTCAAATAGCCAAGAGACTAGCAATTGTTGCACTTTGGTGCATCCAGTGGTACCCGGTGAATCGCCCTTCCATGAAAGCAGTTGTCAGAATGCTAGAAGGACCCTCTGAGAGCTTGATCTTGCCACCCAATCCGTTTGCTTCAACAACTCACACTAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_pol_A_exo1

Protein Analysis

319

Amino Acids

35.32

Weight (kDa)

6.52

Isoelectric Point (pI)

33.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 40 - 298 2.2e-42 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 42 - 296 1.3e-42 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 849
AccB1I GGYRCC 2 cut(s) 614, 849
AccB7I CCANNNNNTGG 1 cut(s) 179
AclWI GGATC 2 cut(s) 481, 494
AcoI YGGCCR 2 cut(s) 564, 747
AcuI CTGAAG 2 cut(s) 327, 657
AfaI GTAC 2 cut(s) 686, 851
AfiI CCNNNNNNNGG 2 cut(s) 179, 261
AgsI TTSAA 4 cut(s) 77, 538, 710, 942
AjnI CCWGG 1 cut(s) 561
AjuI GAANNNNNNNTTGG 2 cut(s) 129, 161
AleI CACNNNNGTG 1 cut(s) 835
AluBI AGCT 6 cut(s) 169, 351, 722, 769, 781, 912
AluI AGCT 6 cut(s) 169, 351, 722, 769, 781, 912
Alw21I GWGCWC 1 cut(s) 325
Alw26I GTCTC 1 cut(s) 808
AlwI GGATC 2 cut(s) 481, 494
AoxI GGCC 3 cut(s) 559, 564, 747
ApeKI GCWGC 2 cut(s) 11, 605
Asp718I GGTACC 1 cut(s) 849
AspS9I GGNCC 1 cut(s) 899
AsuC2I CCSGG 1 cut(s) 854
AsuHPI GGTGA 4 cut(s) 146, 260, 797, 868
AvaII GGWCC 1 cut(s) 899
BalI TGGCCA 1 cut(s) 566
BamHI GGATCC 1 cut(s) 486
BanI GGYRCC 2 cut(s) 614, 849
BbsI GAAGAC 2 cut(s) 342, 588
Bbv12I GWGCWC 1 cut(s) 325
BbvI GCAGC 2 cut(s) 23, 592
BccI CCATC 3 cut(s) 23, 671, 785
BcgI CGANNNNNNTGC 2 cut(s) 779, 813
BciT130I CCWGG 1 cut(s) 563
BclI TGATCA 1 cut(s) 91
BcnI CCSGG 1 cut(s) 854
BcoDI GTCTC 1 cut(s) 808
BfaI CTAG 7 cut(s) 86, 387, 701, 723, 782, 818, 893
BglII AGATCT 1 cut(s) 546
BisI GCNGC 2 cut(s) 12, 606
BlsI GCNGC 2 cut(s) 13, 607
Bme1390I CCNGG 2 cut(s) 563, 854
Bme18I GGWCC 1 cut(s) 899
BmgT120I GGNCC 1 cut(s) 899
BmiI GGNNCC 6 cut(s) 253, 488, 616, 661, 851, 901
BmrFI CCNGG 2 cut(s) 563, 854
BmsI GCATC 3 cut(s) 687, 787, 849
BpiI GAAGAC 2 cut(s) 342, 588
BpmI CTGGAG 1 cut(s) 377
BpuEI CTTGAG 1 cut(s) 191
BpuMI CCSGG 1 cut(s) 854
BsaJI CCNNGG 1 cut(s) 469
BsaWI WCCGGW 1 cut(s) 187
BsaXI ACNNNNNCTCC 2 cut(s) 174, 204
Bsc4I CCNNNNNNNGG 2 cut(s) 179, 261
Bse1I ACTGG 1 cut(s) 844
BseBI CCWGG 1 cut(s) 563
BseDI CCNNGG 1 cut(s) 469
BseGI GGATG 3 cut(s) 420, 702, 840
BseLI CCNNNNNNNGG 2 cut(s) 179, 261
BseMII CTCAG 1 cut(s) 897
BseNI ACTGG 1 cut(s) 844
BseRI GAGGAG 1 cut(s) 774
BseXI GCAGC 2 cut(s) 23, 592
BshFI GGCC 3 cut(s) 561, 566, 749
BshNI GGYRCC 2 cut(s) 614, 849
BsiHKAI GWGCWC 1 cut(s) 325
BsiSI CCGG 2 cut(s) 188, 854
BslI CCNNNNNNNGG 2 cut(s) 179, 261
BsmAI GTCTC 1 cut(s) 808
BsmI GAATGC 1 cut(s) 894
BsnI GGCC 3 cut(s) 561, 566, 749
Bsp1286I GDGCHC 1 cut(s) 325
Bsp1407I TGTACA 1 cut(s) 684
Bsp143I GATC 5 cut(s) 91, 379, 486, 546, 915
BspANI GGCC 3 cut(s) 561, 566, 749
BspCNI CTCAG 1 cut(s) 898
BspLI GGNNCC 6 cut(s) 253, 488, 616, 661, 851, 901
BspPI GGATC 2 cut(s) 481, 494
BspQI GCTCTTC 1 cut(s) 759
BspT107I GGYRCC 2 cut(s) 614, 849
BsrGI TGTACA 1 cut(s) 684
BsrI ACTGG 1 cut(s) 844
BssECI CCNNGG 1 cut(s) 469
BssMI GATC 5 cut(s) 91, 379, 486, 546, 915
BssT1I CCWWGG 1 cut(s) 469
Bst2UI CCWGG 1 cut(s) 563
Bst4CI ACNGT 3 cut(s) 587, 689, 739
Bst6I CTCTTC 1 cut(s) 759
BstAPI GCANNNNNTGC 1 cut(s) 827
BstAUI TGTACA 1 cut(s) 684
BstC8I GCNNGC 2 cut(s) 317, 321
BstDEI CTNAG 1 cut(s) 906
BstF5I GGATG 3 cut(s) 420, 702, 840
BstKTI GATC 5 cut(s) 94, 382, 489, 549, 918
BstMAI GTCTC 1 cut(s) 808
BstMBI GATC 5 cut(s) 91, 379, 486, 546, 915
BstMWI GCNNNNNNNGC 3 cut(s) 621, 806, 827
BstNI CCWGG 1 cut(s) 563
BstNSI RCATGY 1 cut(s) 343
BstSCI CCNGG 2 cut(s) 561, 852
BstV1I GCAGC 2 cut(s) 23, 592
BstV2I GAAGAC 2 cut(s) 342, 588
BstX2I RGATCY 2 cut(s) 486, 546
BstYI RGATCY 2 cut(s) 486, 546
BsuRI GGCC 3 cut(s) 561, 566, 749
BtsCI GGATG 3 cut(s) 420, 702, 840
BtsI GCAGTG 1 cut(s) 396
BtsIMutI CAGTG 4 cut(s) 166, 396, 735, 851
Cac8I GCNNGC 2 cut(s) 317, 321
Cfr13I GGNCC 1 cut(s) 899
Csp6I GTAC 2 cut(s) 685, 850
CviAII CATG 3 cut(s) 340, 530, 871
CviQI GTAC 2 cut(s) 685, 850
DdeI CTNAG 1 cut(s) 906
DpnI GATC 5 cut(s) 93, 381, 488, 548, 917
DpnII GATC 5 cut(s) 91, 379, 486, 546, 915
EaeI YGGCCR 2 cut(s) 564, 747
Eam1104I CTCTTC 1 cut(s) 759
EarI CTCTTC 1 cut(s) 759
Eco130I CCWWGG 1 cut(s) 469
Eco32I GATATC 1 cut(s) 502
Eco47I GGWCC 1 cut(s) 899
Eco57I CTGAAG 2 cut(s) 327, 657
EcoO109I RGGNCCY 1 cut(s) 899
EcoRII CCWGG 1 cut(s) 561
EcoRV GATATC 1 cut(s) 502
EcoT14I CCWWGG 1 cut(s) 469
ErhI CCWWGG 1 cut(s) 469
FaeI CATG 3 cut(s) 343, 533, 874
FalI AAGNNNNNCTT 2 cut(s) 573, 605
FatI CATG 3 cut(s) 339, 529, 870
FauNDI CATATG 1 cut(s) 105
FbaI TGATCA 1 cut(s) 91
Fnu4HI GCNGC 2 cut(s) 12, 606
FokI GGATG 3 cut(s) 427, 709, 827
Fsp4HI GCNGC 2 cut(s) 12, 606
FspBI CTAG 7 cut(s) 86, 387, 701, 723, 782, 818, 893
GluI GCNGC 2 cut(s) 12, 606
GsuI CTGGAG 1 cut(s) 377
HaeIII GGCC 3 cut(s) 561, 566, 749
HapII CCGG 2 cut(s) 188, 854
Hin1II CATG 3 cut(s) 343, 533, 874
HindIII AAGCTT 1 cut(s) 167
HinfI GANTC 6 cut(s) 117, 212, 265, 453, 731, 859
HpaII CCGG 2 cut(s) 188, 854
HphI GGTGA 4 cut(s) 146, 260, 797, 868
Hpy166II GTNNAC 1 cut(s) 685
Hpy188I TCNGA 8 cut(s) 40, 122, 543, 553, 580, 679, 887, 907
Hpy188III TCNNGA 2 cut(s) 70, 356
Hpy8I GTNNAC 1 cut(s) 685
HpyAV CCTTC 5 cut(s) 131, 302, 721, 876, 890
HpyCH4III ACNGT 3 cut(s) 587, 689, 739
HpyCH4IV ACGT 1 cut(s) 279
HpyCH4V TGCA 2 cut(s) 830, 840
HpyF10VI GCNNNNNNNGC 3 cut(s) 621, 806, 827
HpyF3I CTNAG 1 cut(s) 906
HpySE526I ACGT 1 cut(s) 279
Hsp92II CATG 3 cut(s) 343, 533, 874
KpnI GGTACC 1 cut(s) 853
Ksp22I TGATCA 1 cut(s) 91
Kzo9I GATC 5 cut(s) 91, 379, 486, 546, 915
LguI GCTCTTC 1 cut(s) 759
LmnI GCTCC 3 cut(s) 581, 719, 778
Lsp1109I GCAGC 2 cut(s) 23, 592
LweI GCATC 3 cut(s) 687, 787, 849
MaeI CTAG 7 cut(s) 86, 387, 701, 723, 782, 818, 893
MaeII ACGT 1 cut(s) 279
MaeIII GTNAC 2 cut(s) 739, 785
MalI GATC 5 cut(s) 93, 381, 488, 548, 917
MboI GATC 5 cut(s) 91, 379, 486, 546, 915
MboII GAAGA 5 cut(s) 43, 347, 556, 593, 776
MfeI CAATTG 1 cut(s) 822
MflI RGATCY 2 cut(s) 486, 546
MhlI GDGCHC 1 cut(s) 325
MlsI TGGCCA 1 cut(s) 566
MluCI AATT 1 cut(s) 822
MluNI TGGCCA 1 cut(s) 566
MnlI CCTC 9 cut(s) 43, 134, 215, 305, 500, 519, 605, 752, 913
Mox20I TGGCCA 1 cut(s) 566
MscI TGGCCA 1 cut(s) 566
MseI TTAA 2 cut(s) 240, 645
MslI CAYNNNNRTG 2 cut(s) 278, 835
Msp20I TGGCCA 1 cut(s) 566
MspI CCGG 2 cut(s) 188, 854
MspR9I CCNGG 2 cut(s) 563, 854
MunI CAATTG 1 cut(s) 822
Mva1269I GAATGC 1 cut(s) 894
MvaI CCWGG 1 cut(s) 563
MwoI GCNNNNNNNGC 3 cut(s) 621, 806, 827
NciI CCSGG 1 cut(s) 854
NdeI CATATG 1 cut(s) 105
NdeII GATC 5 cut(s) 91, 379, 486, 546, 915
NlaIII CATG 3 cut(s) 343, 533, 874
NlaIV GGNNCC 6 cut(s) 253, 488, 616, 661, 851, 901
NmuCI GTSAC 1 cut(s) 785
NspI RCATGY 1 cut(s) 343
OliI CACNNNNGTG 1 cut(s) 835
PciSI GCTCTTC 1 cut(s) 759
PctI GAATGC 1 cut(s) 894
PfeI GAWTC 6 cut(s) 117, 212, 265, 453, 731, 859
PflMI CCANNNNNTGG 1 cut(s) 179
PkrI GCNGC 2 cut(s) 13, 607
PpuMI RGGWCCY 1 cut(s) 899
Psp5II RGGWCCY 1 cut(s) 899
Psp6I CCWGG 1 cut(s) 561
PspGI CCWGG 1 cut(s) 561
PspN4I GGNNCC 6 cut(s) 253, 488, 616, 661, 851, 901
PspPI GGNCC 1 cut(s) 899
PspPPI RGGWCCY 1 cut(s) 899
PsuI RGATCY 2 cut(s) 486, 546
RsaI GTAC 2 cut(s) 686, 851
RsaNI GTAC 2 cut(s) 685, 850
RseI CAYNNNNRTG 2 cut(s) 278, 835
SapI GCTCTTC 1 cut(s) 759
SaqAI TTAA 2 cut(s) 240, 645
SatI GCNGC 2 cut(s) 12, 606
Sau3AI GATC 5 cut(s) 91, 379, 486, 546, 915
Sau96I GGNCC 1 cut(s) 899
ScrFI CCNGG 2 cut(s) 563, 854
SduI GDGCHC 1 cut(s) 325
SfaNI GCATC 3 cut(s) 687, 787, 849
SinI GGWCC 1 cut(s) 899
SmiMI CAYNNNNRTG 2 cut(s) 278, 835
SmlI CTYRAG 1 cut(s) 206
SmoI CTYRAG 1 cut(s) 206
Sse9I AATT 1 cut(s) 822
SspMI CTAG 7 cut(s) 86, 387, 701, 723, 782, 818, 893
StyD4I CCNGG 2 cut(s) 561, 852
StyI CCWWGG 1 cut(s) 469
TaaI ACNGT 3 cut(s) 587, 689, 739
TaiI ACGT 1 cut(s) 282
TaqI TCGA 2 cut(s) 456, 498
TasI AATT 1 cut(s) 822
TatI WGTACW 1 cut(s) 684
TfiI GAWTC 6 cut(s) 117, 212, 265, 453, 731, 859
Tru1I TTAA 2 cut(s) 240, 645
Tru9I TTAA 2 cut(s) 240, 645
TscAI CASTG 4 cut(s) 166, 403, 742, 851
TseFI GTSAC 1 cut(s) 785
TseI GCWGC 2 cut(s) 11, 605
Tsp45I GTSAC 1 cut(s) 785
TspDTI ATGAA 5 cut(s) 258, 348, 393, 431, 887
TspGWI ACGGA 1 cut(s) 921
TspRI CASTG 4 cut(s) 166, 403, 742, 851
Van91I CCANNNNNTGG 1 cut(s) 179
VpaK11BI GGWCC 1 cut(s) 899
XceI RCATGY 1 cut(s) 343
XcmI CCANNNNNNNNNTGG 1 cut(s) 110
XspI CTAG 7 cut(s) 86, 387, 701, 723, 782, 818, 893
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.