AT1G64520

proteasome assembly

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0005081
Physical Location & Seq
Forward (+)
23956269 .. 23958461
2193 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G64520.1

Sequence Viewer

Length: 804 bp
ATGGATCCGCAGCTAACTGAAGTTTCACAGCAGTTCGAGAGATTCAAGGCGGCGTTTGCGAGGAAGGATTACAATACCTGTAGCGATCTGTTGTCTCAGCTTAAGGTTCTTCTTACCAAATTCACAAGTCTTCCTCCATTGTTCGAGAATTCACCTAATGCAGCCAAGGAATTGACTATTGCAAGGGACATTTATGAGCATGCTGTTGTTCTAAGCGTCAAAACCGAGGATCAAGATGCTTTTGAGAGAGATTTCTTCCAGCTGAAACCTTACTATGTGGATGCCAGGAACCGTATTCCTCAGTCTCCACAGGAGAATCTTATCCTGGGTCTTAACCTTCTGAGGCTGCTGGTACAAAATAGAATTGCTGAATTCCACACCGAGCTTGAATTACTTTCATCAGCTACACTAGAGGATCCTTGCATCAAGCATGCAGTAGAGCTTGAGCAATCCTTCATGGAGGGTGCCTATAACCGTGTGTTGAGTGCTAGACAGACCGCGCCTGATGCAACTTACGTCTATTTCATGGATCTCTTGGCAAAGACCATAAGAGATGAAATAGCTGGATGCAGCGAGAAAGCTTATGATTATGTCTCTATCAGCGATGCCCGGCAGATGTTGTTGTTCTCTTCTGATCAAGAACTCTTAACCTATGTCACAGATGAGCATCCTGAGTGGGAAGTGAAGGAAGGGTTTGTTGTCTTCCAGAAAGCCAAAGAAACTGCACCGTGCAAAGAGATTCCGTCTCTGCAACTCATCAACCAGACTCTAAGCTACGCCAGAGAGCTGGAGCGTATCGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000502 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005838 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0007275 GO:0008150 GO:0008152 GO:0008541 GO:0009056 GO:0009057 GO:0009266 GO:0009314 GO:0009408 GO:0009416 GO:0009507 GO:0009526 GO:0009536 GO:0009628 GO:0009642 GO:0009646 GO:0009647 GO:0009719 GO:0009725 GO:0009733 GO:0009735 GO:0009790 GO:0009791 GO:0009793 GO:0009889 GO:0009908 GO:0009941 GO:0009962 GO:0009987 GO:0010033 GO:0010154 GO:0010498 GO:0016043 GO:0019222 GO:0019538 GO:0019941 GO:0022414 GO:0022607 GO:0022622 GO:0022624 GO:0030163 GO:0031537 GO:0031540 GO:0031967 GO:0031975 GO:0032501 GO:0032502 GO:0032991 GO:0034622 GO:0035966 GO:0042221 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043248 GO:0043632 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0048316 GO:0048364 GO:0048366 GO:0048367 GO:0048528 GO:0048608 GO:0048731 GO:0048825 GO:0048827 GO:0048856 GO:0050789 GO:0050896 GO:0051603 GO:0051788 GO:0061458 GO:0065003 GO:0065007 GO:0071704 GO:0071840 GO:0080090 GO:0090567 GO:0090696 GO:0099402 GO:1901564 GO:1901565 GO:1901575 GO:1902494 GO:1905368 GO:1905369
KEGG Pathways
Metabolic & Signaling
ko03050 ko05169 map03050 map05169
Pfam Domains
Protein Families
CSN8_PSD8_EIF3K

Protein Analysis

267

Amino Acids

30.71

Weight (kDa)

4.81

Isoelectric Point (pI)

33.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAC3_GANP PF03399 79 - 220 1.1e-06 SAC3/GANP family
CSN8_PSD8_EIF3K PF10075 106 - 241 8.8e-29 CSN8/PSMD8/EIF3K family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 464
AccII CGCG 1 cut(s) 500
AciI CCGC 3 cut(s) 8, 50, 498
AclWI GGATC 5 cut(s) 12, 237, 410, 423, 537
AcsI RAATTY 3 cut(s) 119, 148, 371
AcuI CTGAAG 1 cut(s) 39
AfaI GTAC 1 cut(s) 354
AflII CTTAAG 1 cut(s) 101
AgsI TTSAA 2 cut(s) 46, 389
AjnI CCWGG 2 cut(s) 284, 324
Alw26I GTCTC 4 cut(s) 99, 309, 598, 750
AlwI GGATC 5 cut(s) 12, 237, 410, 423, 537
ApeKI GCWGC 4 cut(s) 10, 161, 346, 570
ApoI RAATTY 3 cut(s) 119, 148, 371
AspLEI GCGC 1 cut(s) 502
AsuC2I CCSGG 1 cut(s) 610
AsuHPI GGTGA 1 cut(s) 144
BamHI GGATCC 2 cut(s) 4, 415
BanI GGYRCC 1 cut(s) 464
BbsI GAAGAC 2 cut(s) 122, 694
BbvI GCAGC 4 cut(s) 22, 173, 333, 582
BciT130I CCWGG 2 cut(s) 286, 326
BclI TGATCA 1 cut(s) 634
BcnI CCSGG 1 cut(s) 610
BcoDI GTCTC 4 cut(s) 99, 309, 598, 750
BfaI CTAG 2 cut(s) 410, 489
BfmI CTRYAG 1 cut(s) 79
BfrI CTTAAG 1 cut(s) 101
BisI GCNGC 5 cut(s) 11, 51, 162, 347, 571
BlsI GCNGC 5 cut(s) 12, 52, 163, 348, 572
Bme1390I CCNGG 3 cut(s) 286, 326, 610
BmiI GGNNCC 4 cut(s) 6, 290, 417, 466
BmrFI CCNGG 3 cut(s) 286, 326, 610
BmsI GCATC 7 cut(s) 226, 271, 432, 496, 557, 595, 676
BpiI GAAGAC 2 cut(s) 122, 694
BpuEI CTTGAG 1 cut(s) 464
BpuMI CCSGG 1 cut(s) 610
BsaBI GATNNNNATC 1 cut(s) 666
BsaJI CCNNGG 3 cut(s) 165, 225, 325
Bse8I GATNNNNATC 1 cut(s) 666
BseBI CCWGG 2 cut(s) 286, 326
BseDI CCNNGG 3 cut(s) 165, 225, 325
BseGI GGATG 3 cut(s) 286, 572, 667
BseJI GATNNNNATC 1 cut(s) 666
BseMII CTCAG 4 cut(s) 110, 314, 332, 663
BseXI GCAGC 4 cut(s) 22, 173, 333, 582
BsgI GTGCAG 1 cut(s) 708
Bsh1236I CGCG 1 cut(s) 500
BshNI GGYRCC 1 cut(s) 464
BsiSI CCGG 1 cut(s) 610
BslFI GGGAC 1 cut(s) 200
BsmAI GTCTC 4 cut(s) 99, 309, 598, 750
BsmBI CGTCTC 1 cut(s) 750
BsmFI GGGAC 1 cut(s) 200
Bsp143I GATC 6 cut(s) 4, 85, 229, 415, 529, 634
BspACI CCGC 3 cut(s) 8, 50, 498
BspCNI CTCAG 4 cut(s) 109, 313, 333, 664
BspFNI CGCG 1 cut(s) 500
BspLI GGNNCC 4 cut(s) 6, 290, 417, 466
BspPI GGATC 5 cut(s) 12, 237, 410, 423, 537
BspT107I GGYRCC 1 cut(s) 464
BspTI CTTAAG 1 cut(s) 101
BssECI CCNNGG 3 cut(s) 165, 225, 325
BssMI GATC 6 cut(s) 4, 85, 229, 415, 529, 634
BssT1I CCWWGG 1 cut(s) 165
Bst2UI CCWGG 2 cut(s) 286, 326
Bst4CI ACNGT 3 cut(s) 293, 476, 729
Bst6I CTCTTC 1 cut(s) 634
BstAFI CTTAAG 1 cut(s) 101
BstC8I GCNNGC 2 cut(s) 201, 432
BstDEI CTNAG 6 cut(s) 96, 212, 300, 341, 672, 770
BstF5I GGATG 3 cut(s) 286, 572, 667
BstFNI CGCG 1 cut(s) 500
BstHHI GCGC 1 cut(s) 502
BstKTI GATC 6 cut(s) 7, 88, 232, 418, 532, 637
BstMAI GTCTC 4 cut(s) 99, 309, 598, 750
BstMBI GATC 6 cut(s) 4, 85, 229, 415, 529, 634
BstMWI GCNNNNNNNGC 2 cut(s) 56, 506
BstNI CCWGG 2 cut(s) 286, 326
BstNSI RCATGY 2 cut(s) 203, 434
BstSCI CCNGG 3 cut(s) 284, 324, 608
BstSFI CTRYAG 1 cut(s) 79
BstUI CGCG 1 cut(s) 500
BstV1I GCAGC 4 cut(s) 22, 173, 333, 582
BstV2I GAAGAC 2 cut(s) 122, 694
BstX2I RGATCY 3 cut(s) 4, 415, 529
BstXI CCANNNNNNTGG 1 cut(s) 787
BstYI RGATCY 3 cut(s) 4, 415, 529
BtgZI GCGATG 1 cut(s) 618
BtsCI GGATG 3 cut(s) 286, 572, 667
Cac8I GCNNGC 2 cut(s) 201, 432
CfoI GCGC 1 cut(s) 502
CseI GACGC 1 cut(s) 205
Csp6I GTAC 1 cut(s) 353
CviAII CATG 4 cut(s) 200, 431, 457, 526
CviQI GTAC 1 cut(s) 353
DdeI CTNAG 6 cut(s) 96, 212, 300, 341, 672, 770
DpnI GATC 6 cut(s) 6, 87, 231, 417, 531, 636
DpnII GATC 6 cut(s) 4, 85, 229, 415, 529, 634
Eam1104I CTCTTC 1 cut(s) 634
EarI CTCTTC 1 cut(s) 634
Eco130I CCWWGG 1 cut(s) 165
Eco57I CTGAAG 1 cut(s) 39
EcoRI GAATTC 2 cut(s) 148, 371
EcoRII CCWGG 2 cut(s) 284, 324
EcoT14I CCWWGG 1 cut(s) 165
ErhI CCWWGG 1 cut(s) 165
Esp3I CGTCTC 1 cut(s) 750
FaeI CATG 4 cut(s) 203, 434, 460, 529
FaqI GGGAC 1 cut(s) 200
FatI CATG 4 cut(s) 199, 430, 456, 525
FbaI TGATCA 1 cut(s) 634
Fnu4HI GCNGC 5 cut(s) 11, 51, 162, 347, 571
FokI GGATG 3 cut(s) 293, 579, 654
Fsp4HI GCNGC 5 cut(s) 11, 51, 162, 347, 571
FspBI CTAG 2 cut(s) 410, 489
GlaI GCGC 1 cut(s) 501
GluI GCNGC 5 cut(s) 11, 51, 162, 347, 571
HapII CCGG 1 cut(s) 610
HgaI GACGC 1 cut(s) 205
HhaI GCGC 1 cut(s) 502
Hin1II CATG 4 cut(s) 203, 434, 460, 529
Hin6I GCGC 1 cut(s) 500
HinP1I GCGC 1 cut(s) 500
HindIII AAGCTT 1 cut(s) 579
HinfI GANTC 4 cut(s) 42, 316, 739, 766
HpaII CCGG 1 cut(s) 610
HphI GGTGA 1 cut(s) 144
Hpy188I TCNGA 2 cut(s) 342, 634
Hpy188III TCNNGA 6 cut(s) 37, 145, 233, 638, 671, 706
HpyAV CCTTC 5 cut(s) 58, 347, 463, 679, 683
HpyCH4III ACNGT 3 cut(s) 293, 476, 729
HpyCH4IV ACGT 1 cut(s) 516
HpyCH4V TGCA 9 cut(s) 161, 182, 423, 434, 509, 570, 725, 732, 751
HpyF10VI GCNNNNNNNGC 2 cut(s) 56, 506
HpyF3I CTNAG 6 cut(s) 96, 212, 300, 341, 672, 770
HpySE526I ACGT 1 cut(s) 516
Hsp92II CATG 4 cut(s) 203, 434, 460, 529
HspAI GCGC 1 cut(s) 500
Ksp22I TGATCA 1 cut(s) 634
Kzo9I GATC 6 cut(s) 4, 85, 229, 415, 529, 634
LmnI GCTCC 1 cut(s) 790
Lsp1109I GCAGC 4 cut(s) 22, 173, 333, 582
LweI GCATC 7 cut(s) 226, 271, 432, 496, 557, 595, 676
MaeI CTAG 2 cut(s) 410, 489
MaeII ACGT 1 cut(s) 516
MaeIII GTNAC 1 cut(s) 655
MalI GATC 6 cut(s) 6, 87, 231, 417, 531, 636
MboI GATC 6 cut(s) 4, 85, 229, 415, 529, 634
MboII GAAGA 5 cut(s) 101, 122, 247, 621, 694
MflI RGATCY 3 cut(s) 4, 415, 529
MluCI AATT 6 cut(s) 119, 148, 170, 363, 371, 389
MlyI GAGTC 1 cut(s) 760
MnlI CCTC 7 cut(s) 54, 144, 220, 309, 336, 406, 454
MseI TTAA 3 cut(s) 102, 333, 647
MspA1I CMGCKG 1 cut(s) 262
MspCI CTTAAG 1 cut(s) 101
MspI CCGG 1 cut(s) 610
MspR9I CCNGG 3 cut(s) 286, 326, 610
MvaI CCWGG 2 cut(s) 286, 326
MvnI CGCG 1 cut(s) 500
MwoI GCNNNNNNNGC 2 cut(s) 56, 506
NciI CCSGG 1 cut(s) 610
NdeII GATC 6 cut(s) 4, 85, 229, 415, 529, 634
NlaIII CATG 4 cut(s) 203, 434, 460, 529
NlaIV GGNNCC 4 cut(s) 6, 290, 417, 466
NmuCI GTSAC 1 cut(s) 655
NspI RCATGY 2 cut(s) 203, 434
PaeI GCATGC 2 cut(s) 203, 434
PfeI GAWTC 3 cut(s) 42, 316, 739
PkrI GCNGC 5 cut(s) 12, 52, 163, 348, 572
PleI GAGTC 1 cut(s) 760
PpsI GAGTC 1 cut(s) 760
Psp6I CCWGG 2 cut(s) 284, 324
PspGI CCWGG 2 cut(s) 284, 324
PspN4I GGNNCC 4 cut(s) 6, 290, 417, 466
PsuI RGATCY 3 cut(s) 4, 415, 529
PvuII CAGCTG 1 cut(s) 262
RsaI GTAC 1 cut(s) 354
RsaNI GTAC 1 cut(s) 353
SaqAI TTAA 3 cut(s) 102, 333, 647
SatI GCNGC 5 cut(s) 11, 51, 162, 347, 571
Sau3AI GATC 6 cut(s) 4, 85, 229, 415, 529, 634
SchI GAGTC 1 cut(s) 760
ScrFI CCNGG 3 cut(s) 286, 326, 610
SfaNI GCATC 7 cut(s) 226, 271, 432, 496, 557, 595, 676
SfcI CTRYAG 1 cut(s) 79
SmlI CTYRAG 2 cut(s) 101, 443
SmoI CTYRAG 2 cut(s) 101, 443
SphI GCATGC 2 cut(s) 203, 434
Sse9I AATT 6 cut(s) 119, 148, 170, 363, 371, 389
SsiI CCGC 3 cut(s) 8, 50, 498
SspMI CTAG 2 cut(s) 410, 489
StyD4I CCNGG 3 cut(s) 284, 324, 608
StyI CCWWGG 1 cut(s) 165
TaaI ACNGT 3 cut(s) 293, 476, 729
TaiI ACGT 1 cut(s) 519
TaqI TCGA 2 cut(s) 36, 144
TasI AATT 6 cut(s) 119, 148, 170, 363, 371, 389
TauI GCSGC 1 cut(s) 53
TfiI GAWTC 3 cut(s) 42, 316, 739
Tru1I TTAA 3 cut(s) 102, 333, 647
Tru9I TTAA 3 cut(s) 102, 333, 647
TseFI GTSAC 1 cut(s) 655
TseI GCWGC 4 cut(s) 10, 161, 346, 570
Tsp45I GTSAC 1 cut(s) 655
TspDTI ATGAA 4 cut(s) 387, 445, 514, 570
TspGWI ACGGA 1 cut(s) 732
Vha464I CTTAAG 1 cut(s) 101
XapI RAATTY 3 cut(s) 119, 148, 371
XceI RCATGY 2 cut(s) 203, 434
XspI CTAG 2 cut(s) 410, 489
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.