Rroxscaffold_2G00106010

26S proteasome non-atpase regulatory subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0005081
Physical Location & Seq
Forward (+)
29544295 .. 29544941
647 bp
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UTR
Exon/CDS
Intron
Rroxscaffold_2G00106010.1

Sequence Viewer

Length: 567 bp
ATGATAACACAAATGAGAAGCCTCCCGCCGATGTATGAAGCTACACCTAATGCCGCGGGAGAATTAGCAGTAGCAAGGGACATATATGAGCATGCTTTAACGTCTCTTCCACCGTCCCCTCAAGAGGACCTGATTTTAGGTCTCAATCTGCTGAGGCTCCTTGTGCAGAACAAAACTGATGAGTTCCACACCGAGCTGGAACTGCTTGCATGCCTGTCACCCACTGCATTGGACAACCCTTGCATCAAGCACGCGGTGGAGTTGAAACAGTCCTTAATGGAGGAGGGAGGAGCTTACATAAGTGTGTTGCTTGCTCGGCAGACAATGCCAGATGACATCAGTACTTACACTTATGCAAGATATTTCATGGATCTTCTTGAAAAAACTATCAGAGATCAGGAGGCGGACCCTCCCGATGGGGAAGTAAAATATGATGCCGCATTTTTCGAGAAGGCAAAGGAGTCTGCACCTTGTAAGGAGATACCTTCTCTGCAGTTCATCAATCAAATGCTCAGTTGTGCAACAAAGTTGGGTCAGATTGATGGTTCAGAGAATGATAGCTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000502 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005838 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0007275 GO:0008150 GO:0008152 GO:0008541 GO:0009056 GO:0009057 GO:0009266 GO:0009314 GO:0009408 GO:0009416 GO:0009507 GO:0009526 GO:0009536 GO:0009628 GO:0009642 GO:0009646 GO:0009647 GO:0009719 GO:0009725 GO:0009733 GO:0009735 GO:0009790 GO:0009791 GO:0009793 GO:0009889 GO:0009908 GO:0009941 GO:0009962 GO:0009987 GO:0010033 GO:0010154 GO:0010498 GO:0016043 GO:0019222 GO:0019538 GO:0019941 GO:0022414 GO:0022607 GO:0022622 GO:0022624 GO:0030163 GO:0031537 GO:0031540 GO:0031967 GO:0031975 GO:0032501 GO:0032502 GO:0032991 GO:0034622 GO:0035966 GO:0042221 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043248 GO:0043632 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0048316 GO:0048364 GO:0048366 GO:0048367 GO:0048528 GO:0048608 GO:0048731 GO:0048825 GO:0048827 GO:0048856 GO:0050789 GO:0050896 GO:0051603 GO:0051788 GO:0061458 GO:0065003 GO:0065007 GO:0071704 GO:0071840 GO:0080090 GO:0090567 GO:0090696 GO:0099402 GO:1901564 GO:1901565 GO:1901575 GO:1902494 GO:1905368 GO:1905369
KEGG Pathways
Metabolic & Signaling
ko03050 ko05169 map03050 map05169
Pfam Domains
Protein Families
CSN8_PSD8_EIF3K

Protein Analysis

188

Amino Acids

20.93

Weight (kDa)

4.46

Isoelectric Point (pI)

48.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CSN8_PSD8_EIF3K PF10075 42 - 133 2.7e-07 CSN8/PSMD8/EIF3K family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 56, 254
AciI CCGC 6 cut(s) 26, 54, 56, 254, 404, 438
AclWI GGATC 1 cut(s) 378
AdeI CACNNNGTG 1 cut(s) 256
AfaI GTAC 1 cut(s) 343
AfiI CCNNNNNNNGG 2 cut(s) 124, 416
AgsI TTSAA 2 cut(s) 265, 380
AluBI AGCT 4 cut(s) 41, 196, 293, 561
AluI AGCT 4 cut(s) 41, 196, 293, 561
Alw26I GTCTC 2 cut(s) 108, 146
AlwI GGATC 1 cut(s) 378
AspS9I GGNCC 2 cut(s) 127, 406
AsuHPI GGTGA 1 cut(s) 210
AvaII GGWCC 2 cut(s) 127, 406
BbvCI CCTCAGC 1 cut(s) 152
BccI CCATC 2 cut(s) 410, 536
BcoDI GTCTC 2 cut(s) 108, 146
BfmI CTRYAG 1 cut(s) 491
BisI GCNGC 2 cut(s) 54, 438
BlsI GCNGC 2 cut(s) 55, 439
BmcAI AGTACT 1 cut(s) 343
Bme18I GGWCC 2 cut(s) 127, 406
BmgT120I GGNCC 2 cut(s) 127, 406
BmiI GGNNCC 2 cut(s) 158, 408
BmsI GCATC 2 cut(s) 252, 424
Bpu10I CCTNAGC 1 cut(s) 152
BpuEI CTTGAG 1 cut(s) 105
BsaI GGTCTC 1 cut(s) 146
BsaJI CCNNGG 1 cut(s) 54
Bsc4I CCNNNNNNNGG 2 cut(s) 124, 416
BseDI CCNNGG 1 cut(s) 54
BseLI CCNNNNNNNGG 2 cut(s) 124, 416
BseMII CTCAG 2 cut(s) 143, 526
BseRI GAGGAG 2 cut(s) 296, 303
BsgI GTGCAG 2 cut(s) 185, 450
Bsh1236I CGCG 2 cut(s) 56, 254
BslFI GGGAC 2 cut(s) 92, 100
BslI CCNNNNNNNGG 2 cut(s) 124, 416
BsmAI GTCTC 2 cut(s) 108, 146
BsmBI CGTCTC 1 cut(s) 108
BsmFI GGGAC 2 cut(s) 92, 100
Bso31I GGTCTC 1 cut(s) 146
Bsp143I GATC 2 cut(s) 370, 394
BspACI CCGC 6 cut(s) 26, 54, 56, 254, 404, 438
BspCNI CTCAG 2 cut(s) 144, 525
BspFNI CGCG 2 cut(s) 56, 254
BspLI GGNNCC 2 cut(s) 158, 408
BspMAI CTGCAG 1 cut(s) 495
BspPI GGATC 1 cut(s) 378
BspTNI GGTCTC 1 cut(s) 146
BssECI CCNNGG 1 cut(s) 54
BssMI GATC 2 cut(s) 370, 394
Bst4CI ACNGT 2 cut(s) 114, 270
Bst6I CTCTTC 1 cut(s) 111
BstAPI GCANNNNNTGC 1 cut(s) 325
BstC8I GCNNGC 5 cut(s) 93, 207, 211, 252, 312
BstDEI CTNAG 2 cut(s) 152, 512
BstDSI CCRYGG 1 cut(s) 54
BstFNI CGCG 2 cut(s) 56, 254
BstKTI GATC 2 cut(s) 373, 397
BstMAI GTCTC 2 cut(s) 108, 146
BstMBI GATC 2 cut(s) 370, 394
BstMWI GCNNNNNNNGC 4 cut(s) 163, 202, 316, 325
BstNSI RCATGY 2 cut(s) 95, 213
BstSFI CTRYAG 1 cut(s) 491
BstUI CGCG 2 cut(s) 56, 254
BstX2I RGATCY 1 cut(s) 370
BstXI CCANNNNNNTGG 1 cut(s) 229
BstYI RGATCY 1 cut(s) 370
BtgI CCRYGG 1 cut(s) 54
BtsI GCAGTG 1 cut(s) 222
BtsIMutI CAGTG 1 cut(s) 222
Cac8I GCNNGC 5 cut(s) 93, 207, 211, 252, 312
Cfr13I GGNCC 2 cut(s) 127, 406
Cfr42I CCGCGG 1 cut(s) 57
Csp6I GTAC 1 cut(s) 342
CviAII CATG 3 cut(s) 92, 210, 367
CviJI RGCY 6 cut(s) 21, 41, 157, 196, 293, 561
CviKI_1 RGCY 6 cut(s) 21, 41, 157, 196, 293, 561
CviQI GTAC 1 cut(s) 342
DdeI CTNAG 2 cut(s) 152, 512
DpnI GATC 2 cut(s) 372, 396
DpnII GATC 2 cut(s) 370, 394
DraIII CACNNNGTG 1 cut(s) 256
Eam1104I CTCTTC 1 cut(s) 111
EarI CTCTTC 1 cut(s) 111
EciI GGCGGA 1 cut(s) 419
Eco31I GGTCTC 1 cut(s) 146
Eco47I GGWCC 2 cut(s) 127, 406
EcoO109I RGGNCCY 1 cut(s) 127
Esp3I CGTCTC 1 cut(s) 108
FaeI CATG 3 cut(s) 95, 213, 370
FaqI GGGAC 2 cut(s) 92, 100
FatI CATG 3 cut(s) 91, 209, 366
FauI CCCGC 2 cut(s) 33, 49
Fnu4HI GCNGC 2 cut(s) 54, 438
Fsp4HI GCNGC 2 cut(s) 54, 438
GluI GCNGC 2 cut(s) 54, 438
Hin1II CATG 3 cut(s) 95, 213, 370
HinfI GANTC 1 cut(s) 461
HphI GGTGA 1 cut(s) 210
Hpy188I TCNGA 3 cut(s) 392, 537, 550
Hpy188III TCNNGA 5 cut(s) 122, 377, 398, 413, 448
HpyAV CCTTC 2 cut(s) 445, 495
HpyCH4III ACNGT 2 cut(s) 114, 270
HpyCH4IV ACGT 1 cut(s) 101
HpyCH4V TGCA 8 cut(s) 166, 209, 227, 243, 356, 467, 493, 521
HpyF10VI GCNNNNNNNGC 4 cut(s) 163, 202, 316, 325
HpyF3I CTNAG 2 cut(s) 152, 512
HpySE526I ACGT 1 cut(s) 101
Hsp92II CATG 3 cut(s) 95, 213, 370
KspI CCGCGG 1 cut(s) 57
Kzo9I GATC 2 cut(s) 370, 394
LmnI GCTCC 2 cut(s) 162, 290
LpnPI CCDG 5 cut(s) 143, 182, 227, 342, 383
LweI GCATC 2 cut(s) 252, 424
MaeII ACGT 1 cut(s) 101
MaeIII GTNAC 1 cut(s) 216
MalI GATC 2 cut(s) 372, 396
MboI GATC 2 cut(s) 370, 394
MboII GAAGA 2 cut(s) 98, 365
MflI RGATCY 1 cut(s) 370
MluCI AATT 1 cut(s) 62
MlyI GAGTC 1 cut(s) 470
MnlI CCTC 9 cut(s) 32, 118, 129, 147, 274, 277, 281, 394, 420
MseI TTAA 2 cut(s) 98, 275
MslI CAYNNNNRTG 1 cut(s) 302
MspA1I CMGCKG 1 cut(s) 56
MvnI CGCG 2 cut(s) 56, 254
MwoI GCNNNNNNNGC 4 cut(s) 163, 202, 316, 325
NdeII GATC 2 cut(s) 370, 394
NlaIII CATG 3 cut(s) 95, 213, 370
NlaIV GGNNCC 2 cut(s) 158, 408
NmeAIII GCCGAG 1 cut(s) 295
NmuCI GTSAC 1 cut(s) 216
NspI RCATGY 2 cut(s) 95, 213
PaeI GCATGC 2 cut(s) 95, 213
PkrI GCNGC 2 cut(s) 55, 439
PleI GAGTC 1 cut(s) 469
PpsI GAGTC 1 cut(s) 469
PpuMI RGGWCCY 1 cut(s) 127
Psp5II RGGWCCY 1 cut(s) 127
PspN4I GGNNCC 2 cut(s) 158, 408
PspPI GGNCC 2 cut(s) 127, 406
PspPPI RGGWCCY 1 cut(s) 127
PstI CTGCAG 1 cut(s) 495
PsuI RGATCY 1 cut(s) 370
RsaI GTAC 1 cut(s) 343
RsaNI GTAC 1 cut(s) 342
RseI CAYNNNNRTG 1 cut(s) 302
SacII CCGCGG 1 cut(s) 57
SaqAI TTAA 2 cut(s) 98, 275
SatI GCNGC 2 cut(s) 54, 438
Sau3AI GATC 2 cut(s) 370, 394
Sau96I GGNCC 2 cut(s) 127, 406
ScaI AGTACT 1 cut(s) 343
SchI GAGTC 1 cut(s) 470
SfaNI GCATC 2 cut(s) 252, 424
SfcI CTRYAG 1 cut(s) 491
Sfr303I CCGCGG 1 cut(s) 57
SgrBI CCGCGG 1 cut(s) 57
SinI GGWCC 2 cut(s) 127, 406
SmiMI CAYNNNNRTG 1 cut(s) 302
SmlI CTYRAG 1 cut(s) 120
SmoI CTYRAG 1 cut(s) 120
SphI GCATGC 2 cut(s) 95, 213
Sse9I AATT 1 cut(s) 62
SsiI CCGC 6 cut(s) 26, 54, 56, 254, 404, 438
TaaI ACNGT 2 cut(s) 114, 270
TaiI ACGT 1 cut(s) 104
TaqI TCGA 1 cut(s) 447
TasI AATT 1 cut(s) 62
TatI WGTACW 1 cut(s) 341
TauI GCSGC 2 cut(s) 56, 440
Tru1I TTAA 2 cut(s) 98, 275
Tru9I TTAA 2 cut(s) 98, 275
TscAI CASTG 1 cut(s) 229
TseFI GTSAC 1 cut(s) 216
Tsp45I GTSAC 1 cut(s) 216
TspDTI ATGAA 3 cut(s) 51, 355, 487
TspRI CASTG 1 cut(s) 229
VpaK11BI GGWCC 2 cut(s) 127, 406
XceI RCATGY 2 cut(s) 95, 213
ZrmI AGTACT 1 cut(s) 343
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.