MD14G1132800.v1.1

26S proteasome non-atpase regulatory subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0005081
Physical Location & Seq
Forward (+)
21074628 .. 21077933
3306 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1132800.v1.1.491

Sequence Viewer

Length: 804 bp
ATGGATCCGAAGTTCACAGAGATCGCACAGCTCTTCGAGCGATTCAAAGCTGCATTCGTACGGAGCGATTTCGATACCTGCTCCAATTTCCTCTCCCAGCTCAAGGTTAAGCTGACTGAATTAGGAAGCCTACCGCCGTTGTTTCAAGACACACCTAATGCTGTCAAGGAATTAACATTAGCTAGGGATATATATGAGCATGCTGTTGTTCTTAGTGTTAAGATTGAGGATCAAGATGCATTTGAAAGGGATTTCTTTCAGTTGAAGTCTTATTACGTAGATGCTCGAGATCGTCTTCCACCATCCCCTCAAGAGTACCCAATTTTAGGTCTCAACCTGTTGAGACTCCTTGTCCAGAATAGAATTGCTGAGTTCCACACTGAACTGGAACTTCTTTCATCTGCTGCTTTGGAGAACCCTTGCATTAAGCATGCTGTGGAGCTAGAGCAATCTTTCATGGAGGGGGCTTACAACCGTGTGTTGAGCGCTCGTCAGACAGTACCACACGAGACTTATGTTTATTTCATGGATCTCCTGGCTAAAACAGTCAGGGATGAGTTAGCCGGATGCAGCGAGAAGGCGTATGATTTTCTCTCTATTAAGGATGCCCGTCAAATGTTGCTGTTTCCTTCTGACCGTGAACTTTTGGCATATGTCAGTGAGGATCATCCTGAGTGGGAAGTGAAGAATGATGCGGTCTATTTCCAGAAGGCAAAGGAGTCTGCACCTTGCAAAGAGATACCTTCTTTACAACTCATCAACCAAACTCTCAGCTACGCAAGAGAGTTGGAGCGGATTGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03050 ko05169 map03050 map05169
Pfam Domains
Protein Families
CSN8_PSD8_EIF3K

Protein Analysis

268

Amino Acids

30.83

Weight (kDa)

4.93

Isoelectric Point (pI)

33.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CSN8_PSD8_EIF3K PF10075 105 - 242 9.6e-28 CSN8/PSMD8/EIF3K family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 86
AccBSI CCGCTC 1 cut(s) 793
AciI CCGC 3 cut(s) 134, 695, 793
AclWI GGATC 4 cut(s) 12, 237, 537, 672
AfaI GTAC 3 cut(s) 60, 317, 501
AfeI AGCGCT 1 cut(s) 487
AfiI CCNNNNNNNGG 2 cut(s) 103, 326
AgsI TTSAA 4 cut(s) 46, 146, 245, 265
AhdI GACNNNNNGTC 1 cut(s) 350
AjnI CCWGG 1 cut(s) 534
AloI GAACNNNNNNTCC 1 cut(s) 28
AluBI AGCT 7 cut(s) 31, 50, 100, 112, 182, 442, 774
AluI AGCT 7 cut(s) 31, 50, 100, 112, 182, 442, 774
Alw26I GTCTC 3 cut(s) 335, 337, 503
AlwI GGATC 4 cut(s) 12, 237, 537, 672
Ama87I CYCGRG 1 cut(s) 285
Aor51HI AGCGCT 1 cut(s) 487
ApeKI GCWGC 3 cut(s) 50, 404, 570
AspLEI GCGC 1 cut(s) 488
AvaI CYCGRG 1 cut(s) 285
BamHI GGATCC 1 cut(s) 4
BarI GAAGNNNNNNTAC 2 cut(s) 257, 289
BauI CACGAG 1 cut(s) 506
BbsI GAAGAC 1 cut(s) 287
BbvI GCAGC 3 cut(s) 37, 391, 582
BccI CCATC 1 cut(s) 310
BceAI ACGGC 1 cut(s) 121
BciT130I CCWGG 1 cut(s) 536
BcoDI GTCTC 3 cut(s) 335, 337, 503
BfaI CTAG 2 cut(s) 183, 443
BfoI RGCGCY 1 cut(s) 489
BfuAI ACCTGC 1 cut(s) 86
BisI GCNGC 3 cut(s) 51, 405, 571
BlsI GCNGC 3 cut(s) 52, 406, 572
Bme1390I CCNGG 1 cut(s) 536
BmeRI GACNNNNNGTC 1 cut(s) 350
BmeT110I CYCGRG 1 cut(s) 285
BmiI GGNNCC 1 cut(s) 6
BmrFI CCNGG 1 cut(s) 536
BmsI GCATC 5 cut(s) 226, 271, 557, 595, 682
BpiI GAAGAC 1 cut(s) 287
BpuEI CTTGAG 2 cut(s) 86, 294
BsaAI YACGTR 1 cut(s) 277
BsaI GGTCTC 1 cut(s) 335
Bsc4I CCNNNNNNNGG 2 cut(s) 103, 326
Bse1I ACTGG 1 cut(s) 390
BseBI CCWGG 1 cut(s) 536
BseGI GGATG 5 cut(s) 302, 559, 572, 610, 667
BseLI CCNNNNNNNGG 2 cut(s) 103, 326
BseMII CTCAG 3 cut(s) 360, 663, 784
BseNI ACTGG 1 cut(s) 390
BseXI GCAGC 3 cut(s) 37, 391, 582
BseYI CCCAGC 1 cut(s) 96
BsgI GTGCAG 1 cut(s) 708
BsiHKCI CYCGRG 1 cut(s) 285
BsiSI CCGG 1 cut(s) 564
BsiWI CGTACG 1 cut(s) 58
BslI CCNNNNNNNGG 2 cut(s) 103, 326
BsmAI GTCTC 3 cut(s) 335, 337, 503
BsmI GAATGC 1 cut(s) 53
Bso31I GGTCTC 1 cut(s) 335
BsoBI CYCGRG 1 cut(s) 285
Bsp143I GATC 6 cut(s) 4, 21, 229, 289, 529, 664
BspACI CCGC 3 cut(s) 134, 695, 793
BspCNI CTCAG 3 cut(s) 361, 664, 783
BspLI GGNNCC 1 cut(s) 6
BspMI ACCTGC 1 cut(s) 86
BspPI GGATC 4 cut(s) 12, 237, 537, 672
BspQI GCTCTTC 1 cut(s) 38
BspTNI GGTCTC 1 cut(s) 335
BsrBI CCGCTC 1 cut(s) 793
BsrI ACTGG 1 cut(s) 390
BssMI GATC 6 cut(s) 4, 21, 229, 289, 529, 664
BssSI CACGAG 1 cut(s) 506
Bst2BI CACGAG 1 cut(s) 506
Bst2UI CCWGG 1 cut(s) 536
Bst4CI ACNGT 4 cut(s) 476, 499, 547, 638
Bst6I CTCTTC 1 cut(s) 38
BstBAI YACGTR 1 cut(s) 277
BstC8I GCNNGC 2 cut(s) 201, 432
BstDEI CTNAG 4 cut(s) 212, 369, 672, 770
BstF5I GGATG 5 cut(s) 302, 559, 572, 610, 667
BstH2I RGCGCY 1 cut(s) 489
BstHHI GCGC 1 cut(s) 488
BstKTI GATC 6 cut(s) 7, 24, 232, 292, 532, 667
BstMAI GTCTC 3 cut(s) 335, 337, 503
BstMBI GATC 6 cut(s) 4, 21, 229, 289, 529, 664
BstMWI GCNNNNNNNGC 1 cut(s) 37
BstNI CCWGG 1 cut(s) 536
BstNSI RCATGY 2 cut(s) 203, 434
BstSCI CCNGG 1 cut(s) 534
BstSNI TACGTA 1 cut(s) 277
BstV1I GCAGC 3 cut(s) 37, 391, 582
BstV2I GAAGAC 1 cut(s) 287
BstX2I RGATCY 2 cut(s) 4, 529
BstYI RGATCY 2 cut(s) 4, 529
BtsCI GGATG 5 cut(s) 302, 559, 572, 610, 667
BtsIMutI CAGTG 2 cut(s) 378, 664
BveI ACCTGC 1 cut(s) 86
Cac8I GCNNGC 2 cut(s) 201, 432
CfoI GCGC 1 cut(s) 488
Csp6I GTAC 3 cut(s) 59, 316, 500
CviAII CATG 4 cut(s) 200, 431, 457, 526
CviQI GTAC 3 cut(s) 59, 316, 500
DdeI CTNAG 4 cut(s) 212, 369, 672, 770
DpnI GATC 6 cut(s) 6, 23, 231, 291, 531, 666
DpnII GATC 6 cut(s) 4, 21, 229, 289, 529, 664
DriI GACNNNNNGTC 1 cut(s) 350
Eam1104I CTCTTC 1 cut(s) 38
Eam1105I GACNNNNNGTC 1 cut(s) 350
EarI CTCTTC 1 cut(s) 38
Eco105I TACGTA 1 cut(s) 277
Eco31I GGTCTC 1 cut(s) 335
Eco47III AGCGCT 1 cut(s) 487
Eco88I CYCGRG 1 cut(s) 285
EcoRII CCWGG 1 cut(s) 534
EcoT22I ATGCAT 1 cut(s) 241
FaeI CATG 4 cut(s) 203, 434, 460, 529
FatI CATG 4 cut(s) 199, 430, 456, 525
FauNDI CATATG 1 cut(s) 652
Fnu4HI GCNGC 3 cut(s) 51, 405, 571
FokI GGATG 5 cut(s) 289, 566, 579, 617, 654
Fsp4HI GCNGC 3 cut(s) 51, 405, 571
FspBI CTAG 2 cut(s) 183, 443
GlaI GCGC 1 cut(s) 487
GluI GCNGC 3 cut(s) 51, 405, 571
GsaI CCCAGC 1 cut(s) 100
HaeII RGCGCY 1 cut(s) 489
HapII CCGG 1 cut(s) 564
HhaI GCGC 1 cut(s) 488
Hin1II CATG 4 cut(s) 203, 434, 460, 529
Hin6I GCGC 1 cut(s) 486
HinP1I GCGC 1 cut(s) 486
HinfI GANTC 3 cut(s) 42, 345, 719
HpaII CCGG 1 cut(s) 564
Hpy166II GTNNAC 2 cut(s) 15, 641
Hpy188I TCNGA 3 cut(s) 9, 495, 634
Hpy188III TCNNGA 7 cut(s) 146, 233, 287, 311, 355, 671, 706
Hpy8I GTNNAC 2 cut(s) 15, 641
HpyAV CCTTC 4 cut(s) 571, 639, 703, 753
HpyCH4III ACNGT 4 cut(s) 476, 499, 547, 638
HpyCH4IV ACGT 1 cut(s) 276
HpyCH4V TGCA 6 cut(s) 53, 239, 423, 570, 725, 732
HpyF10VI GCNNNNNNNGC 1 cut(s) 37
HpyF3I CTNAG 4 cut(s) 212, 369, 672, 770
HpySE526I ACGT 1 cut(s) 276
Hsp92II CATG 4 cut(s) 203, 434, 460, 529
HspAI GCGC 1 cut(s) 486
Kzo9I GATC 6 cut(s) 4, 21, 229, 289, 529, 664
LguI GCTCTTC 1 cut(s) 38
LmnI GCTCC 4 cut(s) 63, 86, 439, 790
Lsp1109I GCAGC 3 cut(s) 37, 391, 582
LweI GCATC 5 cut(s) 226, 271, 557, 595, 682
MaeI CTAG 2 cut(s) 183, 443
MaeII ACGT 1 cut(s) 276
MalI GATC 6 cut(s) 6, 23, 231, 291, 531, 666
MbiI CCGCTC 1 cut(s) 793
MboI GATC 6 cut(s) 4, 21, 229, 289, 529, 664
MboII GAAGA 3 cut(s) 25, 287, 697
MflI RGATCY 2 cut(s) 4, 529
MluCI AATT 5 cut(s) 85, 119, 170, 321, 363
MlyI GAGTC 2 cut(s) 339, 728
MmeI TCCRAC 1 cut(s) 768
MnlI CCTC 5 cut(s) 101, 220, 318, 454, 655
Mph1103I ATGCAT 1 cut(s) 241
MseI TTAA 5 cut(s) 108, 173, 219, 426, 600
MspI CCGG 1 cut(s) 564
MspR9I CCNGG 1 cut(s) 536
Mva1269I GAATGC 1 cut(s) 53
MvaI CCWGG 1 cut(s) 536
MwoI GCNNNNNNNGC 1 cut(s) 37
NdeI CATATG 1 cut(s) 652
NdeII GATC 6 cut(s) 4, 21, 229, 289, 529, 664
NlaIII CATG 4 cut(s) 203, 434, 460, 529
NlaIV GGNNCC 1 cut(s) 6
NsiI ATGCAT 1 cut(s) 241
NspI RCATGY 2 cut(s) 203, 434
PaeI GCATGC 2 cut(s) 203, 434
PaeR7I CTCGAG 1 cut(s) 285
PciSI GCTCTTC 1 cut(s) 38
PcsI WCGNNNNNNNCGW 1 cut(s) 63
PctI GAATGC 1 cut(s) 53
PfeI GAWTC 1 cut(s) 42
Pfl23II CGTACG 1 cut(s) 58
PkrI GCNGC 3 cut(s) 52, 406, 572
PleI GAGTC 2 cut(s) 339, 727
PpsI GAGTC 2 cut(s) 339, 727
Ppu21I YACGTR 1 cut(s) 277
Psp6I CCWGG 1 cut(s) 534
PspFI CCCAGC 1 cut(s) 96
PspGI CCWGG 1 cut(s) 534
PspLI CGTACG 1 cut(s) 58
PspN4I GGNNCC 1 cut(s) 6
PsuI RGATCY 2 cut(s) 4, 529
RsaI GTAC 3 cut(s) 60, 317, 501
RsaNI GTAC 3 cut(s) 59, 316, 500
SapI GCTCTTC 1 cut(s) 38
SaqAI TTAA 5 cut(s) 108, 173, 219, 426, 600
SatI GCNGC 3 cut(s) 51, 405, 571
Sau3AI GATC 6 cut(s) 4, 21, 229, 289, 529, 664
SchI GAGTC 2 cut(s) 339, 728
ScrFI CCNGG 1 cut(s) 536
SfaNI GCATC 5 cut(s) 226, 271, 557, 595, 682
Sfr274I CTCGAG 1 cut(s) 285
SlaI CTCGAG 1 cut(s) 285
SmlI CTYRAG 3 cut(s) 101, 285, 309
SmoI CTYRAG 3 cut(s) 101, 285, 309
SnaBI TACGTA 1 cut(s) 277
SphI GCATGC 2 cut(s) 203, 434
Sse9I AATT 5 cut(s) 85, 119, 170, 321, 363
SsiI CCGC 3 cut(s) 134, 695, 793
SspMI CTAG 2 cut(s) 183, 443
StyD4I CCNGG 1 cut(s) 534
TaaI ACNGT 4 cut(s) 476, 499, 547, 638
TaiI ACGT 1 cut(s) 279
TaqI TCGA 3 cut(s) 36, 72, 286
TasI AATT 5 cut(s) 85, 119, 170, 321, 363
TfiI GAWTC 1 cut(s) 42
Tru1I TTAA 5 cut(s) 108, 173, 219, 426, 600
Tru9I TTAA 5 cut(s) 108, 173, 219, 426, 600
TscAI CASTG 2 cut(s) 385, 664
TseI GCWGC 3 cut(s) 50, 404, 570
TspDTI ATGAA 3 cut(s) 387, 445, 514
TspGWI ACGGA 1 cut(s) 76
TspRI CASTG 2 cut(s) 385, 664
XceI RCATGY 2 cut(s) 203, 434
XhoI CTCGAG 1 cut(s) 285
XspI CTAG 2 cut(s) 183, 443
Zsp2I ATGCAT 1 cut(s) 241
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.