FvH4_6g31620

26S proteasome non-atpase regulatory subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0005081
Physical Location & Seq
Reverse (-)
24722826 .. 24723572
747 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g31620.t1

Sequence Viewer

Length: 747 bp
ATGGCTGACCCAGTACTACAAGAGGTGCACCTGAACTTCGATCTTTTTAGAAAGGCGTATGAACAGAAAGATTTCAATGCATGCCCCGAGCTCCTCACGAAGCTCAAGGCCATGCTAGCACAACTAAGAAGCCTCCCGCCGATGTATGAAGCTACACCAAATGCGGCGGGAGAACTAACAGTAGCAAGGGACATATATGAGCATGCTGTTGTTCTTAGCTTGAAGACAGAGAATGAGGAAGCATTCGAGAGGGATTTTCTTCAGTTGAAGCAGTACTATTATGCCGCGGTTGGTACCGGTCGCCTTCCACCGTCCCCTCAAGAGGACCTGCTATTAGCTCTCAACCTGCTGAGGCTCCTTGTGCAGAACAGAACTGATGAGTTCCACGACGAGCTGGAGCTGCTTGTATGCATGTCACCCGATGCATTGGACAACCCTTGCATCAAGCATGTCGTGGAGTTGGAGAAGTCCTTAATGGAGGAGGGAGGAGCTTACGTAACTACGTTGCTTGCTCGGCAAGCAATGCCAGATGACATTAGTACTTATACTTATGCAAATTATTTTATGGATCTTCTTGAAAAAACTATCAGAGATCAGGGGGATGTAAATAATAATGATGCGGTGTTTTTCGAGAAGGCGAAGGAGTCTGCACCTTGCAAGGAGATACCATCTCTGCAGCTAATCAATCAGATGCTCAGTCATGCAACAAAGTCGGATCAGATCCATGGTTCAGAGAACGATAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000502 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005838 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0007275 GO:0008150 GO:0008152 GO:0008541 GO:0009056 GO:0009057 GO:0009266 GO:0009314 GO:0009408 GO:0009416 GO:0009507 GO:0009526 GO:0009536 GO:0009628 GO:0009642 GO:0009646 GO:0009647 GO:0009719 GO:0009725 GO:0009733 GO:0009735 GO:0009790 GO:0009791 GO:0009793 GO:0009889 GO:0009908 GO:0009941 GO:0009962 GO:0009987 GO:0010033 GO:0010154 GO:0010498 GO:0016043 GO:0019222 GO:0019538 GO:0019941 GO:0022414 GO:0022607 GO:0022622 GO:0022624 GO:0030163 GO:0031537 GO:0031540 GO:0031967 GO:0031975 GO:0032501 GO:0032502 GO:0032991 GO:0034622 GO:0035966 GO:0042221 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043248 GO:0043632 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0048316 GO:0048364 GO:0048366 GO:0048367 GO:0048528 GO:0048608 GO:0048731 GO:0048825 GO:0048827 GO:0048856 GO:0050789 GO:0050896 GO:0051603 GO:0051788 GO:0061458 GO:0065003 GO:0065007 GO:0071704 GO:0071840 GO:0080090 GO:0090567 GO:0090696 GO:0099402 GO:1901564 GO:1901565 GO:1901575 GO:1902494 GO:1905368 GO:1905369
KEGG Pathways
Metabolic & Signaling
ko03050 ko05169 map03050 map05169
Pfam Domains
Protein Families
CSN8_PSD8_EIF3K

Protein Analysis

249

Amino Acids

28.1

Weight (kDa)

4.62

Isoelectric Point (pI)

43.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CSN8_PSD8_EIF3K PF10075 108 - 198 9.8e-06 CSN8/PSMD8/EIF3K family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 336, 354
Acc65I GGTACC 1 cut(s) 293
AccB1I GGYRCC 1 cut(s) 293
AccII CGCG 1 cut(s) 287
AciI CCGC 6 cut(s) 137, 164, 167, 285, 287, 620
AclWI GGATC 3 cut(s) 576, 715, 723
AcuI CTGAAG 1 cut(s) 245
AfaI GTAC 4 cut(s) 15, 275, 295, 541
AfiI CCNNNNNNNGG 1 cut(s) 322
AgeI ACCGGT 1 cut(s) 296
AgsI TTSAA 4 cut(s) 76, 223, 268, 578
Alw21I GWGCWC 2 cut(s) 30, 93
Alw44I GTGCAC 1 cut(s) 26
AlwI GGATC 3 cut(s) 576, 715, 723
Ama87I CYCGRG 1 cut(s) 86
AoxI GGCC 1 cut(s) 108
ApaLI GTGCAC 1 cut(s) 26
ApeKI GCWGC 2 cut(s) 400, 676
AsiGI ACCGGT 1 cut(s) 296
Asp700I GAANNNNTTC 1 cut(s) 71
Asp718I GGTACC 1 cut(s) 293
AspS9I GGNCC 1 cut(s) 325
AsuHPI GGTGA 1 cut(s) 408
AsuNHI GCTAGC 1 cut(s) 115
AvaI CYCGRG 1 cut(s) 86
AvaII GGWCC 1 cut(s) 325
BaeGI GKGCMC 1 cut(s) 30
BanI GGYRCC 1 cut(s) 293
BanII GRGCYC 1 cut(s) 93
BbsI GAAGAC 1 cut(s) 230
Bbv12I GWGCWC 2 cut(s) 30, 93
BbvCI CCTCAGC 1 cut(s) 350
BbvI GCAGC 2 cut(s) 387, 688
BccI CCATC 1 cut(s) 676
BcgI CGANNNNNNTGC 2 cut(s) 693, 727
BfaI CTAG 2 cut(s) 116, 745
BfmI CTRYAG 1 cut(s) 674
BfuAI ACCTGC 2 cut(s) 336, 354
BisI GCNGC 4 cut(s) 165, 285, 401, 677
BlsI GCNGC 4 cut(s) 166, 286, 402, 678
BmcAI AGTACT 3 cut(s) 15, 275, 541
Bme18I GGWCC 1 cut(s) 325
BmeT110I CYCGRG 1 cut(s) 86
BmgT120I GGNCC 1 cut(s) 325
BmiI GGNNCC 2 cut(s) 295, 356
BmrI ACTGGG 1 cut(s) 5
BmsI GCATC 4 cut(s) 412, 450, 607, 681
BmtI GCTAGC 1 cut(s) 119
BmuI ACTGGG 1 cut(s) 5
BpiI GAAGAC 1 cut(s) 230
BpmI CTGGAG 1 cut(s) 416
Bpu10I CCTNAGC 1 cut(s) 350
BpuEI CTTGAG 2 cut(s) 89, 303
BsaAI YACGTR 1 cut(s) 496
BsaJI CCNNGG 2 cut(s) 285, 724
BsaWI WCCGGW 1 cut(s) 296
BsaXI ACNNNNNCTCC 2 cut(s) 480, 510
Bsc4I CCNNNNNNNGG 1 cut(s) 322
Bse118I RCCGGY 1 cut(s) 296
Bse1I ACTGG 1 cut(s) 11
Bse3DI GCAATG 1 cut(s) 528
BseDI CCNNGG 2 cut(s) 285, 724
BseGI GGATG 1 cut(s) 607
BseLI CCNNNNNNNGG 1 cut(s) 322
BseMI GCAATG 1 cut(s) 528
BseMII CTCAG 2 cut(s) 341, 709
BseNI ACTGG 1 cut(s) 11
BseRI GAGGAG 3 cut(s) 83, 494, 501
BseSI GKGCMC 1 cut(s) 30
BseXI GCAGC 2 cut(s) 387, 688
BsgI GTGCAG 2 cut(s) 383, 633
Bsh1236I CGCG 1 cut(s) 287
Bsh1285I CGRYCG 1 cut(s) 301
BshFI GGCC 1 cut(s) 110
BshNI GGYRCC 1 cut(s) 293
BshTI ACCGGT 1 cut(s) 296
BsiEI CGRYCG 1 cut(s) 301
BsiHKAI GWGCWC 2 cut(s) 30, 93
BsiHKCI CYCGRG 1 cut(s) 86
BsiSI CCGG 1 cut(s) 297
BslFI GGGAC 2 cut(s) 203, 298
BslI CCNNNNNNNGG 1 cut(s) 322
BsmFI GGGAC 2 cut(s) 203, 298
BsmI GAATGC 1 cut(s) 242
BsnI GGCC 1 cut(s) 110
BsoBI CYCGRG 1 cut(s) 86
Bsp1286I GDGCHC 2 cut(s) 30, 93
Bsp143I GATC 5 cut(s) 40, 568, 592, 715, 720
Bsp19I CCATGG 1 cut(s) 724
BspACI CCGC 6 cut(s) 137, 164, 167, 285, 287, 620
BspANI GGCC 1 cut(s) 110
BspCNI CTCAG 2 cut(s) 342, 708
BspFNI CGCG 1 cut(s) 287
BspLI GGNNCC 2 cut(s) 295, 356
BspMAI CTGCAG 1 cut(s) 678
BspMI ACCTGC 2 cut(s) 336, 354
BspOI GCTAGC 1 cut(s) 119
BspPI GGATC 3 cut(s) 576, 715, 723
BspT107I GGYRCC 1 cut(s) 293
BsrDI GCAATG 1 cut(s) 528
BsrFI RCCGGY 1 cut(s) 296
BsrI ACTGG 1 cut(s) 11
BssAI RCCGGY 1 cut(s) 296
BssECI CCNNGG 2 cut(s) 285, 724
BssMI GATC 5 cut(s) 40, 568, 592, 715, 720
BssT1I CCWWGG 1 cut(s) 724
Bst4CI ACNGT 2 cut(s) 181, 312
BstAPI GCANNNNNTGC 1 cut(s) 523
BstBAI YACGTR 1 cut(s) 496
BstC8I GCNNGC 5 cut(s) 82, 117, 204, 510, 519
BstDEI CTNAG 4 cut(s) 125, 215, 350, 695
BstDSI CCRYGG 2 cut(s) 285, 724
BstF5I GGATG 1 cut(s) 607
BstFNI CGCG 1 cut(s) 287
BstKTI GATC 5 cut(s) 43, 571, 595, 718, 723
BstMBI GATC 5 cut(s) 40, 568, 592, 715, 720
BstMCI CGRYCG 1 cut(s) 301
BstMWI GCNNNNNNNGC 6 cut(s) 116, 361, 400, 514, 518, 523
BstNSI RCATGY 4 cut(s) 84, 206, 415, 452
BstSFI CTRYAG 1 cut(s) 674
BstSLI GKGCMC 1 cut(s) 30
BstSNI TACGTA 1 cut(s) 496
BstUI CGCG 1 cut(s) 287
BstV1I GCAGC 2 cut(s) 387, 688
BstV2I GAAGAC 1 cut(s) 230
BstX2I RGATCY 2 cut(s) 568, 720
BstYI RGATCY 2 cut(s) 568, 720
BsuRI GGCC 1 cut(s) 110
BtgI CCRYGG 2 cut(s) 285, 724
BtsCI GGATG 1 cut(s) 607
BveI ACCTGC 2 cut(s) 336, 354
Cac8I GCNNGC 5 cut(s) 82, 117, 204, 510, 519
Cfr10I RCCGGY 1 cut(s) 296
Cfr13I GGNCC 1 cut(s) 325
Cfr42I CCGCGG 1 cut(s) 288
Csp6I GTAC 4 cut(s) 14, 274, 294, 540
CspAI ACCGGT 1 cut(s) 296
CviAII CATG 7 cut(s) 81, 112, 203, 412, 449, 701, 725
CviQI GTAC 4 cut(s) 14, 274, 294, 540
DdeI CTNAG 4 cut(s) 125, 215, 350, 695
DpnI GATC 5 cut(s) 42, 570, 594, 717, 722
DpnII GATC 5 cut(s) 40, 568, 592, 715, 720
Ecl136II GAGCTC 1 cut(s) 91
Eco105I TACGTA 1 cut(s) 496
Eco130I CCWWGG 1 cut(s) 724
Eco24I GRGCYC 1 cut(s) 93
Eco47I GGWCC 1 cut(s) 325
Eco53kI GAGCTC 1 cut(s) 91
Eco57I CTGAAG 1 cut(s) 245
Eco88I CYCGRG 1 cut(s) 86
EcoICRI GAGCTC 1 cut(s) 91
EcoO109I RGGNCCY 1 cut(s) 325
EcoT14I CCWWGG 1 cut(s) 724
EcoT22I ATGCAT 3 cut(s) 82, 413, 427
EcoT38I GRGCYC 1 cut(s) 93
ErhI CCWWGG 1 cut(s) 724
FaeI CATG 7 cut(s) 84, 115, 206, 415, 452, 704, 728
FaqI GGGAC 2 cut(s) 203, 298
FatI CATG 7 cut(s) 80, 111, 202, 411, 448, 700, 724
FauI CCCGC 2 cut(s) 144, 160
Fnu4HI GCNGC 4 cut(s) 165, 285, 401, 677
FokI GGATG 1 cut(s) 614
FriOI GRGCYC 1 cut(s) 93
Fsp4HI GCNGC 4 cut(s) 165, 285, 401, 677
FspBI CTAG 2 cut(s) 116, 745
GluI GCNGC 4 cut(s) 165, 285, 401, 677
GsuI CTGGAG 1 cut(s) 416
HaeIII GGCC 1 cut(s) 110
HapII CCGG 1 cut(s) 297
Hin1II CATG 7 cut(s) 84, 115, 206, 415, 452, 704, 728
HinfI GANTC 1 cut(s) 644
HpaII CCGG 1 cut(s) 297
HphI GGTGA 1 cut(s) 408
Hpy166II GTNNAC 1 cut(s) 28
Hpy188I TCNGA 5 cut(s) 590, 690, 715, 720, 733
Hpy188III TCNNGA 5 cut(s) 97, 247, 320, 575, 631
Hpy8I GTNNAC 1 cut(s) 28
Hpy99I CGWCG 1 cut(s) 392
HpyAV CCTTC 3 cut(s) 314, 628, 634
HpyCH4III ACNGT 2 cut(s) 181, 312
HpyCH4IV ACGT 2 cut(s) 495, 503
HpyF10VI GCNNNNNNNGC 6 cut(s) 116, 361, 400, 514, 518, 523
HpyF3I CTNAG 4 cut(s) 125, 215, 350, 695
HpySE526I ACGT 2 cut(s) 495, 503
Hsp92II CATG 7 cut(s) 84, 115, 206, 415, 452, 704, 728
KpnI GGTACC 1 cut(s) 297
KspI CCGCGG 1 cut(s) 288
Kzo9I GATC 5 cut(s) 40, 568, 592, 715, 720
LmnI GCTCC 4 cut(s) 96, 360, 397, 488
LpnPI CCDG 8 cut(s) 24, 44, 310, 341, 359, 380, 540, 581
Lsp1109I GCAGC 2 cut(s) 387, 688
LweI GCATC 4 cut(s) 412, 450, 607, 681
MaeI CTAG 2 cut(s) 116, 745
MaeII ACGT 2 cut(s) 495, 503
MaeIII GTNAC 2 cut(s) 414, 496
MalI GATC 5 cut(s) 42, 570, 594, 717, 722
MboI GATC 5 cut(s) 40, 568, 592, 715, 720
MboII GAAGA 3 cut(s) 235, 251, 563
MflI RGATCY 2 cut(s) 568, 720
MhlI GDGCHC 2 cut(s) 30, 93
MluCI AATT 1 cut(s) 556
MlyI GAGTC 1 cut(s) 653
MmeI TCCRAC 2 cut(s) 441, 693
Mph1103I ATGCAT 3 cut(s) 82, 413, 427
MroXI GAANNNNTTC 1 cut(s) 71
MseI TTAA 1 cut(s) 473
MspA1I CMGCKG 1 cut(s) 287
MspI CCGG 1 cut(s) 297
Mva1269I GAATGC 1 cut(s) 242
MvnI CGCG 1 cut(s) 287
MwoI GCNNNNNNNGC 6 cut(s) 116, 361, 400, 514, 518, 523
NcoI CCATGG 1 cut(s) 724
NdeII GATC 5 cut(s) 40, 568, 592, 715, 720
NheI GCTAGC 1 cut(s) 115
NlaIII CATG 7 cut(s) 84, 115, 206, 415, 452, 704, 728
NlaIV GGNNCC 2 cut(s) 295, 356
NmeAIII GCCGAG 1 cut(s) 493
NmuCI GTSAC 1 cut(s) 414
NsiI ATGCAT 3 cut(s) 82, 413, 427
NspI RCATGY 4 cut(s) 84, 206, 415, 452
PaeI GCATGC 2 cut(s) 84, 206
PctI GAATGC 1 cut(s) 242
PdmI GAANNNNTTC 1 cut(s) 71
PinAI ACCGGT 1 cut(s) 296
PkrI GCNGC 4 cut(s) 166, 286, 402, 678
PleI GAGTC 1 cut(s) 652
PpsI GAGTC 1 cut(s) 652
Ppu21I YACGTR 1 cut(s) 496
PpuMI RGGWCCY 1 cut(s) 325
Psp124BI GAGCTC 1 cut(s) 93
Psp5II RGGWCCY 1 cut(s) 325
PspN4I GGNNCC 2 cut(s) 295, 356
PspPI GGNCC 1 cut(s) 325
PspPPI RGGWCCY 1 cut(s) 325
PstI CTGCAG 1 cut(s) 678
PsuI RGATCY 2 cut(s) 568, 720
RsaI GTAC 4 cut(s) 15, 275, 295, 541
RsaNI GTAC 4 cut(s) 14, 274, 294, 540
SacI GAGCTC 1 cut(s) 93
SacII CCGCGG 1 cut(s) 288
SaqAI TTAA 1 cut(s) 473
SatI GCNGC 4 cut(s) 165, 285, 401, 677
Sau3AI GATC 5 cut(s) 40, 568, 592, 715, 720
Sau96I GGNCC 1 cut(s) 325
ScaI AGTACT 3 cut(s) 15, 275, 541
SchI GAGTC 1 cut(s) 653
SduI GDGCHC 2 cut(s) 30, 93
SfaNI GCATC 4 cut(s) 412, 450, 607, 681
SfcI CTRYAG 1 cut(s) 674
Sfr303I CCGCGG 1 cut(s) 288
SgrBI CCGCGG 1 cut(s) 288
SinI GGWCC 1 cut(s) 325
SmlI CTYRAG 2 cut(s) 104, 318
SmoI CTYRAG 2 cut(s) 104, 318
SnaBI TACGTA 1 cut(s) 496
SphI GCATGC 2 cut(s) 84, 206
Sse9I AATT 1 cut(s) 556
SsiI CCGC 6 cut(s) 137, 164, 167, 285, 287, 620
SspMI CTAG 2 cut(s) 116, 745
SstI GAGCTC 1 cut(s) 93
StyI CCWWGG 1 cut(s) 724
TaaI ACNGT 2 cut(s) 181, 312
TaiI ACGT 2 cut(s) 498, 506
TaqI TCGA 3 cut(s) 39, 246, 630
TasI AATT 1 cut(s) 556
TatI WGTACW 3 cut(s) 13, 273, 539
TauI GCSGC 2 cut(s) 167, 287
Tru1I TTAA 1 cut(s) 473
Tru9I TTAA 1 cut(s) 473
TseFI GTSAC 1 cut(s) 414
TseI GCWGC 2 cut(s) 400, 676
Tsp45I GTSAC 1 cut(s) 414
TspDTI ATGAA 2 cut(s) 75, 162
VneI GTGCAC 1 cut(s) 26
VpaK11BI GGWCC 1 cut(s) 325
XceI RCATGY 4 cut(s) 84, 206, 415, 452
XmnI GAANNNNTTC 1 cut(s) 71
XspI CTAG 2 cut(s) 116, 745
ZrmI AGTACT 3 cut(s) 15, 275, 541
Zsp2I ATGCAT 3 cut(s) 82, 413, 427
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.